Updated on 2026/03/23
博士（農学） （ 2009.3 名古屋大学 ）
酵素
ピリドキサールリン酸
D-アミノ酸
ビタミンB6
代謝
葉酸
名古屋大学大学院 生命農学研究科 講師
2016.6
Country：Japan
名古屋大学大学院 生命農学研究科 助教
2010.4 - 2016.5
Country：Japan
Research Fellow of the Japan Society for the Promotion of Science (JSPS)
2008.4 - 2010.3
Country：Japan
京都大学化学研究所
2009.4 - 2010.3
Nagoya University Graduate School of Bioagricultural Sciences Division of Applied Biochemistry
2004.4 - 2009.3
Country： Japan
Nagoya University Faculty of Agriculture
2000.4 - 2004.3
Country： Japan
日本生化学会
日本農芸化学会
日本ビタミン学会
D-アミノ酸学会
日本ビタミン学会 代議員
2025.11 - 2027.10
Committee type：Academic society
日本農芸化学会中部支部 庶務幹事
2024.4 - 2026.3
Committee type：Academic society
日本農芸化学会中部支部 会計幹事
2020.4 - 2022.3
Committee type：Academic society
農芸化学奨励賞
2021.3 日本農芸化学会 高度に保存されたビタミンB6結合タンパク質の機能解析と応用展開
JB論文賞
2017 公益社団法人 日本生化学会
A new branch of mammalian vitamin B(6) metabolism: AKR1C-mediated conversion of pyridoxal to pyridoxine and 4-pyridoxolactone. Reviewed Open Access
Kito N, Kitaura Y, Iino K, Toriumi K, Noh H, Ogawa N, Arai M, Hemmi H, Ito T
The FEBS journal 2026.2
Harnessing the Power of SMART Single-Molecule Display for Enzyme Evolution: A Focus on Oxidase. Reviewed
Munaweera K, Odake N, Halim HP, Ikeda K, Zhu B, Camagna M, Ito T, Kitaguchi T, Nemoto N, Nakano H, Damnjanović J
ACS synthetic biology 2026.2
Deciphering the properties and reaction mechanism of anhydromevalonate phosphate decarboxylase, a prenylated flavin mononucleotide-dependent enzyme in the archaeal mevalonate pathway. Reviewed Open Access
Ishikawa R, Matsushima N, Ishimine S, Nakamoto H, Hayakawa H, Yasuno Y, Shinada T, Kawaide H, Ito T, Hemmi H
The FEBS journal 2026.1
Enhanced isoprenoid production in Escherichia coli cells harboring the archaeal mevalonate pathway. Reviewed Open Access
Takahashi T, Saito T, Dohgami I, Ito T, Hemmi H
Biochemistry and biophysics reports Vol. 44 page： 102307 2025.12
Suicide substrate reaction-like modification of mouse serine racemase with L-serine. Reviewed Open Access
Hata A, Ito T, Mori H, Ogawa T, Kurihara T, Hemmi H, Yoshimura T
Journal of biochemistry Vol. 177 ( 6 ) page： 437 - 445 2025.5
Identification and Characterization of a Novel d-Branched-Chain Amino Acids Importer from Lactobacillus fermentum. Reviewed Open Access
Aoki K, Mutaguchi Y, Hemmi H, Yoshimura T, Ito T
Chembiochem : a European journal of chemical biology Vol. 26 ( 6 ) page： e202401075 2025.3
Effects of producing high levels of hyperthermophile-specific C(25),C(25)-archaeal membrane lipids in Escherichia coli. Reviewed Open Access
Yoshida R, Motoyama K, Ito T, Hemmi H
Biochemical and biophysical research communications Vol. 729 page： 150349 2024.10
Identification of YigL as a PLP/PNP phosphatase in Escherichia coli. Reviewed Open Access
Matsuo H, Yamada N, Hemmi H, Ito T
Applied and environmental microbiology Vol. 90 ( 9 ) page： e0127024 2024.9
Archaeal mevalonate pathway in the uncultured bacterium Candidatus Promineifilum breve belonging to the phylum Chloroflexota. Reviewed Open Access
Kanno K, Kuriki R, Yasuno Y, Shinada T, Ito T, Hemmi H
Applied and environmental microbiology Vol. 90 ( 8 ) page： e0110624 2024.8
D-amino acid auxotrophic <i>Escherichia coli</i> strain for <i>in vivo</i> functional cloning of novel D-amino acid synthetic enzyme Reviewed Open Access
Ito, T; Muto, N; Sakagami, H; Tanaka, M; Hemmi, H; Yoshimura, T
FEBS JOURNAL Vol. 290 ( 11 ) page： 2895 - 2908 2023.6
A [4Fe-4S] cluster resides at the active center of phosphomevalonate dehydratase, a key enzyme in the archaeal modified mevalonate pathway Reviewed Open Access
Komeyama, M; Kanno, K; Mino, H; Yasuno, Y; Shinada, T; Ito, T; Hemmi, H
FRONTIERS IN MICROBIOLOGY Vol. 14 page： 1150353 2023.3
Role of the conserved pyridoxal 5'-phosphate-binding protein YggS/PLPBP in vitamin B-6 and amino acid homeostasis Invited Reviewed Open Access
Ito Tomokazu
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY Vol. 86 ( 9 ) page： 1183 - 1191 2022.8
Crystal structure of mevalonate 3,5-bisphosphate decarboxylase reveals insight into the evolution of decarboxylases in the mevalonate metabolic pathways Reviewed International coauthorship Open Access
Aoki, M; Vinokur, J; Motoyama, K; Ishikawa, R; Collazo, M; Cascio, D; Sawaya, MR; Ito, T; Bowie, JU; Hemmi, H
JOURNAL OF BIOLOGICAL CHEMISTRY Vol. 298 ( 7 ) page： 102111 2022.7
Identification and biochemical characterization of a heteromeric <i>cis</i>-prenyltransferase from the thermophilic archaeon <i>Archaeoglobus fulgidus</i> Reviewed Open Access
Sompiyachoke, K; Nagasaka, A; Ito, T; Hemmi, H
JOURNAL OF BIOCHEMISTRY Vol. 171 ( 6 ) page： 641 - 651 2022.5
Identification and characterization of a serine racemase in the silkworm <i>Bombyx mori</i> Reviewed
Tanaka, Y; Yoshimura, T; Hakamata, M; Saito, C; Sumitani, M; Sezutsu, H; Hemmi, H; Ito, T
JOURNAL OF BIOCHEMISTRY Vol. 172 ( 1 ) page： 17 - 28 2022.3
Mechanism of Pyridoxine 5′-Phosphate Accumulation in Pyridoxal 5′-Phosphate-Binding Protein Deficiency Reviewed International coauthorship Open Access
Ito, T; Ogawa, H; Hemmi, H; Downs, DM; Yoshimura, T
JOURNAL OF BACTERIOLOGY Vol. 204 ( 3 ) page： e0052121 2022.3
Isopentenyl diphosphate/dimethylallyl diphosphate-specific Nudix hydrolase from the methanogenic archaeon <i>Methanosarcina mazei</i> Reviewed Open Access
Ishibashi, Y; Matsushima, N; Ito, T; Hemmi, H
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY Vol. 86 ( 2 ) page： 246 - 253 2022.1
Urinary l-erythro-β-hydroxyasparagine-a novel serine racemase inhibitor and substrate of the Zn2+-dependent d-serine dehydratase. Reviewed
Ito T, Tono M, Kitaura Y, Hemmi H, Yoshimura T
Bioscience reports Vol. 41 ( 4 ) 2021.4
ビタミンB6・アミノ酸恒常性に関与するピリドキサールリン酸結合タンパク質 Invited Reviewed
伊藤智和
Vitamins 2021
The Role of YggS in Vitamin B6 Homeostasis in Salmonella enterica Is Informed by Heterologous Expression of Yeast <i>SNZ3</i>. Reviewed International coauthorship Open Access
Vu HN, Ito T, Downs DM
Journal of bacteriology Vol. 202 ( 22 ) 2020.10
Mechanism of eukaryotic serine racemase-catalyzed serine dehydration Reviewed
Ito Tomokazu, Matsuoka Mai, Goto Masaru, Watanabe Soichiro, Mizobuchi Taichi, Matsushita Kazuma, Nasu Ryoma, Hemmi Hisashi, Yoshimura Tohru
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS Vol. 1868 ( 9 ) page： 140460 2020.9
Pyridoxal Reductase, PdxI, Is Critical for Salvage of Pyridoxal in Escherichia coli Reviewed International coauthorship Open Access
Ito Tomokazu, Downs Diana M.
JOURNAL OF BACTERIOLOGY Vol. 202 ( 12 ) 2020.6
Inhibition of glycine cleavage system by pyridoxine 5′-phosphate causes synthetic lethality in <i>glyA yggS</i> and <i>serA yggS</i> in <i>Escherichia coli</i> Reviewed International coauthorship Open Access
Ito, T; Hori, R; Hemmi, H; Downs, DM; Yoshimura, T
MOLECULAR MICROBIOLOGY Vol. 113 ( 1 ) page： 270 - 284 2020.1
Conserved Pyridoxal 5′-Phosphate-Binding Protein YggS Impacts Amino Acid Metabolism through Pyridoxine 5′-Phosphate in <i>Escherichia</i> <i>coli</i> Reviewed International coauthorship Open Access
Ito, T; Yamamoto, K; Hori, R; Yamauchi, A; Downs, DM; Hemmi, H; Yoshimura, T
APPLIED AND ENVIRONMENTAL MICROBIOLOGY Vol. 85 ( 11 ) 2019.6
Conserved Pyridoxal 5'-Phosphate-Binding Protein YggS Impacts Amino Acid Metabolism through Pyridoxine 5'-Phosphate in Escherichia coli. Reviewed International coauthorship
Ito T, Yamamoto K, Hori R, Yamauchi A, Downs DM, Hemmi H, Yoshimura T
Appl Environ Microbiol. Vol. 85 ( 11 ) 2019.5
Urinary D-serine level as a predictive biomarker for deterioration of renal function in patients with atherosclerotic risk factors Reviewed Open Access
Iwakawa Hidehiro, Makabe Shin, Ito Tomokazu, Yoshimura Tohru, Watanabe Hiroyuki
BIOMARKERS Vol. 24 ( 2 ) page： 159 - 165 2019.2
Inhibition of glycine cleavage system by pyridoxine 5'-phosphate causes synthetic lethality in glyA yggS and serA yggS in Escherichia coli Reviewed International coauthorship
Ito T,Hori R,Hemmi H, Downs DM, Yoshimura T
Molecular Microbiology page： in press 2019
Modified mevalonate pathway of the archaeon Aeropyrum pernix proceeds via trans-anhydromevalonate 5-phosphate Reviewed Open Access
Hayakawa Hajime, Motoyama Kento, Sobue Fumiaki, Ito Tomokazu, Kawaide Hiroshi, Yoshimura Tohru, Hemmi Hisashi
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA Vol. 115 ( 40 ) page： 10034 - 10039 2018.10
D-Serine Metabolism and Its Importance in Development of Dictyostelium discoideum Reviewed Open Access
Ito Tomokazu, Hamauchi Natsuki, Hagi Taisuke, Morohashi Naoya, Hemmi Hisashi, Sato Yukie G., Saito Tamao, Yoshimura Tohru
FRONTIERS IN MICROBIOLOGY Vol. 9 page： 784 2018.4
Production of Ophthalmic Acid Using Engineered Escherichia coli. Reviewed Open Access
Ito T, Tokoro M, Hori R, Hemmi H, Yoshimura T
Applied and environmental microbiology Vol. 84 ( 7 ) 2018.4
Hayashi Y, Ito T, Yoshimura T, Hemmi H
Bioscience, biotechnology, and biochemistry Vol. 82 ( 6 ) page： 993 - 1002 2018
Ito Tomokazu, Yu Zhuoer, Yoshino Issei, Hirozawa Yurina, Yamamoto Kana, Shinoda Kiyotugu, Watanabe Akira, Hemmi Hisashi, Asada Yasuhiko, Yoshimura Tohru
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY Vol. 65 ( 30 ) page： 6131 - 6139 2017.8
Development of a versatile method for targeted gene deletion and insertion by using the pyrF gene in the psychrotrophic bacterium, Shewanella livingstonensis Ac10 Reviewed
Ito T., Gong C., Kawamoto J., Kurihara T.
Journal of Bioscience and Bioengineering. page： doi:10.1016/j.jbiosc.2016.06.004 2016
mTORC1 is involved in the regulation of BCAA catabolism in mouse heart Reviewed
Zhen H., Kitaura Y., Kadota Y., Ishikawa T., Kondo Y., Xu M., Morishita Y., Ota M., Ito T., Shimomura Y
FEBS Open Bio. ( 6 ) page： 43-49 2016
Serine racemase is involved in D-aspartate biosynthesis. Reviewed Open Access
Ito T., Hayashida M., Kobayashi S., Muto N., Hayashi A., Yoshimura T., Mori H.
Journal of Biochemistry page： 160,345-353 2016
Ophthalmic acid accumulation in an Escherichia coli mutant lacking the conserved pyridoxal 5' phosphate-binding protein YggS Reviewed
Ito T., Yamauchi A., Hemmi H., Yoshimura T.
Journal of Bioscience and Bioengineering Vol. 122 page： 689-693 2016
Is D-aspartate produced by glutamic-oxaloacetic transaminase-1 like 1 (Got1l1), a putative aspartate racemase? Reviewed
Tanaka-Hayashi A., Hayashi S., Inoue R., Ito T., Kouno K., Yoshida T., Watanabe M., Yoshimura T., Mori H
( 47 ) page： 79-85 2015
Role of the aminotransferase domain in Bacillus subtilis GabR, a pyridoxal 5'-phosphate-dependent transcriptional regulator Reviewed Open Access
Okuda K., Kato S., Ito T., Shiraki S., Kawase Y., Goto M., Kawashima S., Hemmi H., Fukada H., Yoshimura T
Molecular Microbiology page： 245-257 2015
PEGylated D-Serine Dehydratase as a D-Serine Reucing Agent Reviewed
Ito T., Takada H., Isobe K., Suzuki M., Kitaura Y., Hemmi H., Matsuda T., Sasabe J., Yoshimura T
Journal of Parmacetuical and Biomedical Analysis 2015
Domain characterization of Bacillus subtilis GabR, a pyridoxal 5'-phosphate-dependent transcriptional regulator Reviewed
Journal of Biochemistry page： pii: mvv040 2015
A New Member of MocR/GabR-type PLP-Binding Regulator of D-Alanyl-D-Alanine Ligase in Brevibacillus brevis Reviewed
Takenaka T., Ito T., Miyahara I., Hemmi H., Yoshimura T
FEBS J 2015
Reaction mechanism of Zn2+-dependent D-serine dehydratase Reviewed
Tomokazu Ito, Mai Matsuoka, Kazushi Koga, Hisashi Hemmi, and Tohru Yoshimura
The Journal of Biochemistry page： -- 2014
Reaction mechanism of eukaryotic serine racemase
Vitamins Vol. 88 ( 8 ) page： 425-428 2014
Vitami B6-dependet enzymes involved in D-serine metabolism: serine racemase and D-serine dehydratase
Vitamins Vol. 88 ( 9 ) page： 462-468 2014
Reaction mechanism of yeast D-serine dehydratase Open Access
Vitamins Vol. 88 ( 11 ) page： 576-579 2014
A Conserved Pyridoxal Protein that Regulates Ile and Val Metabolism. Reviewed Open Access
Ito T, Iimori J, Takayama S, Moriyama A, Yamauchi A, Hemmi H, Yoshimura T
Journal of Bacteriology 2013
Metal ion dependency of serine racemase from Dictyostelium discoideum Reviewed
Ito T, Murase H, Maekawa M, Goto M, Hayashi S, Saito H, Maki M, Hemmi H, Yoshimura T.
Amino Acids 2012
A highly sensitive enzymatic assay for D- and total serine detection using D-serine dehydratase from Saccharomyces cerevisiae Reviewed
Naka T, Ito T, Hemmi H, Yoshimura T
J. Mol. Catal. B Enzym. Vol. 67 page： 150-154 2011
哺乳類のアスパラギン酸ラセマーゼ
伊藤智和、吉村徹
Vol. 85 page： 661-662 2011
右手型アミノ酸の役割 Open Access
伊藤智和
生物工学会誌 Vol. 88 page： 613 2010
The implication of YggT of Escherichia coli in osmotic regulation Open Access
Ito T, Uozumi N, Nakamura T, Takayama S, Matsuda N, Aiba H, Hemmi H, Yoshimura T
Biosci. Biotechnol. Biochem. Vol. 73 page： 2698-2704 2009
A novel zinc-dependent D-serine dehydratase from Saccharomyces cerevisiae Reviewed
Ito T, Hemmi H, Kataoka K, Mukai Y, Yoshimura T
Biochem J. Vol. 409 page： 399-406 2008
Saccharomyces cerevisiaeの新奇D-セリンデヒドラターゼとD-セリン定量への応用 Open Access
伊藤智和,吉村徹
Vol. 82 page： 349-351 2008
Enzymatic assay of D-serine using D-serine dehydratase from Saccharomyces cerevisiae Reviewed
Ito T, Takahashi K, Naka T, Hemmi H, Yoshimura T
Anal Biochem. Vol. 371 page： 167-172 2007
哺乳類における新たなB6代謝経路・酵素の発見 Invited
伊藤智和
日本農芸化学会2026年度大会 2026.3.10 日本農芸化学会
ビタミンB6代謝・制御に関与する多様な酵素の同定と展開 Invited
伊藤智和
第98回日本生化学会大会 2025.11.3 日本生化学会大会
葉酸尾部構造の多様性について
伊藤智和
ビタミンB研究委員会 2025.9.4 ビタミンB研究委員会
哺乳類のビタミンB6代謝経路
伊藤智和
ビタミンB研究委員会 2025.6.28
新規コク味物質オフタルミン酸の発酵生産法の開発と代謝制御機構の解析
2016.7 - 2017.6
学内共同研究
ヒト尿中D-セリン比に着目した腎障害早期検出法の開発とその評価
2015.1 - 2015.12
Ａ－ＳＴＥＰ「ＦＳステージ 探索タイプ」
Grant type：Competitive
ブナシメジに存在するユニークなD-アミノ酸：同定と生理機能の解明
2015 - 2017.3
「修飾型」葉酸の生物分布と分子機能の解明
Grant number：24K08699 2024.4 - 2027.3
科学研究費助成事業 基盤研究(C)
Authorship：Principal investigator Grant type：Competitive
Grant number：24K01698 2024.4 - 2028.3
科学研究費助成事業 基盤研究(B)
北浦 靖之, 伊藤 智和, 高原 照直, 和田 恵梨, 柴田 貴広
Authorship：Coinvestigator(s) Grant type：Competitive
タンパク質含量の異なる食餌を摂取させ、アミノ酸代謝フローの追跡を行い、脂肪酸合成への関与を明らかとする。in vitroでの膵臓ランゲルハンス島を用いたアミノ酸刺激実験により、内分泌ホルモンの分泌に必要なアミノ酸を同定し、アミノ酸感知システムの制御タンパク質などの関与を明らかにし、阻害剤などを用いたin vivoでの実験により、それらの関与を検証する。また、in vitroでの肝細胞を用いた内分泌ホルモンの刺激実験により、変化する代謝物などを明らかにし、阻害剤などを用いたin vivoでの実験により、それらの関与を検証する。
Effective fermentative production of isoprenoid via oxygen-tolerant archaeal mevalonate pathway
Grant number：23K26819 2024.4 - 2026.3
Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)
Authorship：Coinvestigator(s) Grant type：Competitive
ビタミンB6の生体内恒常性維持機構の解明
2021.4 - 2022.3
公益財団法人発酵研究所 公益財団法人発酵研究所 2021年度 一般研究助成
Authorship：Principal investigator Grant type：Other
Grant amount：\3000000
非天然・分割型代謝経路の構築によるビタミンB6の発酵生産
2021.4 - 2022.3
ロッテ財団 第8回（2021年度）奨励研究助成
Authorship：Principal investigator Grant type：Other
Grant amount：\3000000
ビタミンB6新規サルベージ経路と輸送系の同定
2020.4 - 2022.3
公益財団法人 旭硝子財団 公益財団法人 旭硝子財団 奨励研究
Authorship：Principal investigator Grant type：Competitive
Grant amount：\2000000
Elucidation of the function of a conserved pyridoxal phosphate-binding protein involved in the homeostasis of amino acid metabolism International coauthorship
Grant number：17KK0153 2018 - 2020
Grants-in-Aid for Scientific Research Fund for the Promotion of Joint International Research (Fostering Joint International Research)
Ito Tomokazu
Authorship：Principal investigator Grant type：Competitive
Grant amount：\14560000 （ Direct Cost: \11200000 、 Indirect Cost：\3360000 ）
Loss or mutation of YggS/PROSC protein, a pyridoxal phosphate (PLP)-binding protein, disrupts a wide variety of metabolic systems, especially amino acid and vitamin B6 metabolism. This study has demonstrated that the YggS/PPROSC protein plays an important role in the homeostasis of vitamin B6, especially pyridoxine phosphate (PNP), in various organisms. In E. coli, the abnormal accumulation of PNP inhibits the glycine cleavage system and various metabolic pathways, including amino acid metabolism. The present study suggests that the YggS/PROSC protein may be involved in the conversion process between PLP and pyridoxamine phosphate (PMP).
D-amino acid biological system in eukaryote
Grant number：16K18686 2016.4 - 2018.3
Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B)
Ito Tomokazu
Authorship：Principal investigator
Grant amount：\4160000 （ Direct Cost: \3200000 、 Indirect Cost：\960000 ）
We analyzed the catalytic and regulatory mechanisms of D-Asp synthetic activity of mammalian serine racemase (SR). Data inidicaed that SR is one of mammalian D-Asp biosynthetic enzymes. In addition, we analyzed urinary D-Ser using our D-, and L-Ser enzymatic assay method. The results obtained here suggested that D-Ser levels could be used as a predictable biomarker of renal function reduction in some situations. In addition, we studied the molecular mechanism of developmental abnormalities of Dsd-deficient Dicsyotelium strains and revealed the involvment of D-Ser in cAMP signaling. We found a novel Dsd substrate D-amino acid in the fruiting body of Bunashimeji mushroom, and identified it as (2R, 3S) 2-amino-3,4-dihydroxybutanoic acid.
真核生物におけるD型アミノ酸の機能と制御機構の解明
2012.4 - 2015.3
科学研究費補助金 若手研究(B)
Authorship：Principal investigator
D-セリンデヒドラターゼ及びその利用
伊藤智和、吉村徹
Advanced On-the-job Training
2025
Fundamentals of Chemistry II
2025
Biophysical Chemistry 2
2025
バイオインフォマティクス演習（学生実験）
2025
応用生命科学実験実習
2025
応用生命科学実験実習
2023
専門セミナー
2021
修士論文研究1-4
2021
生命農学演習1-4
2021
卒業論文研究
2021
情報リテラシー
2021
応用生命科学実験実習(実験基礎・分子生物・バイオインフォマティクス）
2021
専門セミナー
2020
論文査読 International contribution
Role(s)：Peer review
2026.3
論文査読 International contribution
Role(s)：Peer review
2026.2
