研究者詳細 - 井上　直子

論文 - 井上　直子

分割表示 93 件中 61 - 80 件目／全件表示 >>

Role of cell-death ligand-receptor system of granulosa cells in selective follicular atresia in porcine ovary. 招待有り査読有り

57 巻頁： 169-175 2011年

Expression and function of apoptosis inhibitor FOXO3 in guranulosa cells during follicular atresia 査読有り

57 巻頁： 151-158 2011年

卵胞発育の中枢制御機構招待有り

井上直子、上野山賀久、大蔵聡、束村博子、前多敬一郎

Hormone Frontier in Gynecology 18 巻 ( 4 ) 頁： 391-394 2011年

キスペプチン/メタスチン-繁殖を制御する新規神経ペプチド招待有り

大蔵聡、上野山賀久、冨川順子、井上直子、束村博子、前多敬一郎

日本獣医師学会雑誌 64 巻 ( 1 ) 頁： 39-44 2011年

キスペプチンによる繁殖機能制御機構招待有り

上野山賀久、大蔵聡、井上直子、前多敬一郎、束村博子

臨床科学 40 巻 ( 2 ) 頁： 113-118 2011年

Molecular characterization and estrogen regulation of hypothalamic KISS1 gene in pig. 査読有り

Tomikawa J, Homma T, Tajima S, Shibata T, Inamoto Y, Takase K, Inoue N, Ohkura S, Uenoyama Y, Maeda K, Tsukamura H.

Biol Reprod 82 巻頁： 313-319 2010年

Activation of Neuropeptide FF Receptors by Kisspeptin Receptor ligands. 査読有り

Oishi S, Misu R, Tomita K, Setuda S, Masuda R, Ohno H, Naniwa Y, Ieda N, Inoue N, Ohkura S, Uenoyama Y, Tsukamura H, Maeda K-I, Hirasawa A, Tsujimoto G, Fujii N.

ACS Medicinal Chemistry letters 2 巻 ( 1 ) 頁： 53-57 2010年

Movement of lyle's flying fox (pteropus lylei) in central thailand.

Hondo E, Inoue N, Maeda K, Rerkamnuaychoke W, Duengkae P.

Journal of wildlife in Thailand 17 巻 ( 1 ) 頁： 55-63 2010年

Physiological role of metastin/kisspeptin in regulating gonadotropin-releasing hormone (GnRH) secretion in female rats. 査読有り

Ohkura S, Uenoyama Y, Yamada S, Homma T, Takase K, Inoue N, Maeda KI, Tsukamura H.

Peptides 30 巻頁： 49-56 2009年

Possible role of oestrogen in pubertal increase of Kiss1/kisspeptin expression in discrete hypothalamic areas of female rats. 査読有り

Takase K, Uenoyama Y, Inoue N, Matsui H, Yamada S, Shimizu M, Homma T, Tomikawa J, Kanda S, Matsumoto H, Oka Y, Tsukamura H, Maeda KI.

J Neuroendocrinol 21 巻頁： 527-537 2009年

cFLIP Regulates Death Receptor-mediated Apoptosis in an Ovarian Granulosa Cell Line by Inhibiting Procaspase-8 Cleavage. 査読有り

Journal of Reproduction and Development 54 巻頁： 314-20 2008年

Role of MAdCAM-1 and its ligand on the homing of transplanted hematopoietic cells in irradiated mice. 査読有り

Tada T, Inoue N, Widayati DT, Fukuta K.

Exp Anim 57 巻頁： 347-356 2008年

Changes in Expression and Localization of X-linked Inhibitor of Apoptosis Protein (XIAP) in Follicular Granulosa Cells During Atresia in Porcine Ovaries. 査読有り

Yuan Cheng, Naoko Inoue, Fuko Matsuda-Minehata, Yasufumi Goto, Akihisa Maeda, Noboru Manabe.

Journal of Reproduction and Development 54 巻頁： 454-459 2008年

Molecular cloning of a porcine (Sus scrofa) apoptosis inhibitory ligand, netrin-1, and its receptor, p53RDL1. 査読有り

Maeda A, Matsuda F, Goto Y, Cheng Y, Gonda H, Inoue N, Nakagawa S, Manabe N.

Journal of Reproduction and Development 54 巻頁： 275-280 2008年

視床下部－下垂体－性腺軸のマスターレギュレーターとしてのメタスチン（キスペプチン）招待有り

束村博子、上野山賀久、山田俊児、冨川順子、井上直子、大蔵聡、前多敬一郎

ホルモンと臨床 56 巻 ( 8 ) 頁： 771-777 2008年

Molecular Characteristics of Porcine Fas-associated Death Domain (FADD) and Procaspase-8 査読有り

Inoue N, Matsuda-Minehata F, Goto Y, Sakamaki K, Manabe N

Journal of Reproduction and Devevelopment 53 巻頁： 427-436 2007年

担当区分：筆頭著者記述言語：英語掲載種別：研究論文（学術雑誌）

To reveal the intracellular signal transduction molecules in granulosa cell apoptosis in porcine ovarian follicles, I cloned the porcine Fas-associated death domain (FADD), an adaptor protein for the cell death receptor, and procaspase-8, an initiator caspase. Porcine FADD (pFADD) was 636 bp (211 amino acids: aa) long and showed 74.0 and 65.4% homology with human and murine FADD, respectively. Porcine procaspase-8 (pprocaspase-8) was 1,431 bp (476 aa) long and 70.6 and 63.4% homologous with human and murine procaspase-8, respectively. To confirm the apoptosis-inducing abilities, I constructed pFADD- and pprocaspase-8-cDNA expression vectors with enhanced green fluorescence protein (EGFP), and then transfected them into human uterine cervix tumor (HeLa-K), human granulosa-cell derived (KGN), murine granulosa-derived tumor (KK1), and porcine granulosa-derived (JC410) cells. When pFADD and pprocaspase-8 were over-expressed, cell death was induced in these transfected cells. But when the caspase-inhibitor p35 was co-transfected, cell death was inhibited. The pFADD and pprocaspase-8 genes are well conserved, as are the physiological functions of their products.

The Role of Interleukin-6 in the Regulation of Granulosa Cell Apoptosis during Follicular Atresia in Pig Ovaries. 査読有り

Maeda A, Inoue N, Matsuda-Minehata F, Goto Y, Cheng Y, Manabe N.

Journal of Reproduction and Development 53 巻 2007年

Anti-apoptotic activity of porcine cFLIP in ovarian granulosa cell lines. 査読有り

Matsuda-Minehata F, Goto Y, Inoue N, Sakamaki K, Chedrese PJ, Manabe N.

Mol Reprod Dev. 74 巻頁： 1165-1170 2007年

Changes in expression of interleukin-6 receptors in granulosa cells during follicular atresia in pig ovaries. 査読有り

Akihisa Maeda, Yasufumi Goto, Fuko Matsuda-Minehata, Yuan Cheng, Naoko Inoue, Noboru Manabe.

J Reprod Dev 53 巻頁： 727-736 2007年

*Changes in expression levels and localization of Fas ligand and Fas during atresia in porcine ovarian follicles. 査読有り

Naoko Inoue, Akihisa Maeda, Fuko Matsuda-Minehata, Katsuhiro Fukuta, Noboru Manabe.

Journal of Reproduction and Devevelopment 52 巻 2006年

担当区分：筆頭著者記述言語：英語掲載種別：研究論文（学術雑誌）

To reveal the mechanisms regulating the selective atresia of follicles in porcine ovaries, I examined the changes in the mRNA and protein levels of cell-death ligand, Fas/APO-1/CD95 ligand (FasL), and its receptor, Fas/APO-1/CD95 (Fas), and the localization of the proteins in granulosa cells during follicular atresia using the reverse transcription-polymerase chain reaction (RT-PCR), Western blotting, and immunohistochemical techniques, respectively. Trace levels of FasL mRNA and protein were detected in the granulosa cells of healthy follicles; however, weak levels were detected in those of early atretic follicles, and the levels increased during atresia. Trace/weak levels of Fas mRNA and protein were detected in the granulosa cells of healthy follicles. Fas protein was located in the cytoplasmic area, not in cell membrane area, indicating that it has no activity in regard to inducing apoptosis. When apoptosis commences in granulosa cells, Fas moves from the cytoplasmic to cell membrane area. FasL and Fas mRNAs and proteins in granulosa cells were upregulated during follicular atresia. The FasL and Fas system may play a crucial role in the regulation of apoptosis in granulosa cells during selective follicular atresia in porcine ovaries.

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