Updated on 2022/04/22

写真a

 
INOUE Naoko
 
Organization
Graduate School of Bioagricultural Sciences Department of Animal Sciences Lecturer
Graduate School
Graduate School of Bioagricultural Sciences
Undergraduate School
School of Agricultural Sciences Department of Bioresource Sciences
Title
Lecturer
External link

Degree 1

  1. 博士(農学) ( 東京大学 ) 

Research Interests 1

  1. Morphology, Reproduction

Research Areas 3

  1. Others / Others  / Basic Veterinary Science/Basic Animal Science

  2. Others / Others  / Applied Animal Science

  3. Others / Others  / Laboratory Animal Studies

Current Research Project and SDGs 5

  1. 哺乳類卵巣における卵胞退行機構の解明

  2. 食虫目スンクスにおける妊娠維持機構の解析

  3. 交尾排卵動物モデル・スンクスを用いた排卵の神経メカニズムの解明

  4. 食虫目スンクスを用いた新規な遺伝子改変モデル動物の開発

  5. 哺乳類における排卵制御メカニズム

Research History 5

  1. Nagoya University   Lecturer

    2015.3

  2. 名古屋大学大学院生命農学研究科    特任准教授

    2014.4 - 2015.2

      More details

    Country:Japan

  3. 名古屋大学大学院生命農学研究科・助教

    2007.4 - 2014.3

      More details

    Country:Japan

  4. 名古屋大学大学院生命農学研究科・助手

    2004.4 - 2007.3

      More details

    Country:Japan

  5. Nagoya University   Lecturer

Education 3

  1. Kyoto University   Graduate School, Division of Agriculture

    2002.4 - 2004.3

      More details

    Country: Japan

  2. Kyoto University   Graduate School, Division of Agriculture

    2000.4 - 2002.3

      More details

    Country: Japan

  3. Nagoya University   Faculty of Agriculture

    1996.4 - 2000.3

      More details

    Country: Japan

Professional Memberships 7

  1. 日本繁殖生物学会   評議員、将来計画検討委員会委員長

    2012.4

  2. 日本畜産学会

  3. 日本獣医学会

  4. 日本内分泌学会

    2014.3

  5. 日本繁殖生物学会

  6. 日本畜産学会

  7. 日本獣医学会

▼display all

Awards 2

  1. 日本繁殖生物学会奨励賞

    2010.9   日本繁殖生物学会  

     More details

    Country:Japan

  2. The JRD Outstanding Paper Award

    2007.4  

     More details

    Country:Japan

 

Papers 88

  1. Central somatostatin-somatostatin receptor 2 signaling mediates lactational suppression of luteinizing hormone release via the inhibition of glutamatergic interneurons during late lactation in rats.

    Sugimoto A, Tsuchida H, Nagae M, Inoue N, Uenoyama Y, Tsukamura H

    The Journal of reproduction and development     2022.3

     More details

    Language:English  

    DOI: 10.1262/jrd.2022-009

    PubMed

  2. Cellular and molecular mechanisms regulating the KNDy neuronal activities to generate and modulate GnRH pulse in mammals

    Ikegami Kana, Watanabe Youki, Nakamura Sho, Goto Teppei, Inoue Naoko, Uenoyama Yoshihisa, Tsukamura Hiroko

    FRONTIERS IN NEUROENDOCRINOLOGY   Vol. 64   page: 100968   2022.1

     More details

    Language:Japanese   Publisher:Frontiers in Neuroendocrinology  

    Accumulating findings during the past decades have demonstrated that the hypothalamic arcuate kisspeptin neurons are supposed to be responsible for pulsatile release of gonadotropin-releasing hormone (GnRH) to regulate gametogenesis and steroidogenesis in mammals. The arcuate kisspeptin neurons express neurokinin B (NKB) and dynorphin A (Dyn), thus, the neurons are also referred to as KNDy neurons. In the present article, we mainly focus on the cellular and molecular mechanisms underlying GnRH pulse generation, that is focused on the action of NKB and Dyn and an interaction between KNDy neurons and astrocytes to control GnRH pulse generation. Then, we also discuss the factors that modulate the activity of KNDy neurons and consequent pulsatile GnRH/LH release in mammals.

    DOI: 10.1016/j.yfrne.2021.100968

    Web of Science

    Scopus

    PubMed

  3. Kisspeptin neurons as a key player bridging the endocrine system and sexual behavior in mammals

    Nakamura Sho, Watanabe Youki, Goto Teppei, Ikegami Kana, Inoue Naoko, Uenoyama Yoshihisa, Tsukamura Hiroko

    FRONTIERS IN NEUROENDOCRINOLOGY   Vol. 64   page: 100952   2022.1

     More details

    Language:Japanese   Publisher:Frontiers in Neuroendocrinology  

    Reproductive behaviors are sexually differentiated: for example, male rodents show mounting behavior, while females in estrus show lordosis behavior as sex-specific sexual behaviors. Kisspeptin neurons govern reproductive function via direct stimulation of gonadotropin-releasing hormone (GnRH) and subsequent gonadotropin release for gonadal steroidogenesis in mammals. First, we discuss the role of hypothalamic kisspeptin neurons as an indispensable regulator of sexual behavior by stimulating the synthesis of gonadal steroids, which exert “activational effects” on the behavior in adulthood. Second, we discuss the central role of kisspeptin neurons that are directly involved in neural circuits controlling sexual behavior in adulthood. We then focused on the role of perinatal hypothalamic kisspeptin neurons in the induction of perinatal testosterone secretion for its “organizational effects” on masculinization/defeminization of the male brain in rodents during a critical period. We subsequently concluded that kisspeptin neurons are key players in bridging the endocrine system and sexual behavior in mammals.

    DOI: 10.1016/j.yfrne.2021.100952

    Web of Science

    Scopus

    PubMed

  4. Central mu-Opioid Receptor Antagonism Blocks Glucoprivic LH Pulse Suppression and Gluconeogenesis/Feeding in Female Rats

    Tsuchida Hitomi, Kawai Narumi, Yamada Koki, Takizawa Marina, Inoue Naoko, Uenoyama Yoshihisa, Tsukamura Hiroko

    ENDOCRINOLOGY   Vol. 162 ( 10 )   2021.10

     More details

    Language:Japanese   Publisher:Endocrinology (United States)  

    Energetic status often affects reproductive function, glucose homeostasis, and feeding in mammals. Malnutrition suppresses pulsatile release of the gonadotropin-releasing hormone (GnRH)/luteinizing hormone (LH) and increases gluconeogenesis and feeding. The present study aims to examine whether β-endorphin-μ-opioid receptor (MOR) signaling mediates the suppression of pulsatile GnRH/LH release and an increase in gluconeogenesis/feeding induced by malnutrition. Ovariectomized female rats treated with a negative feedback level of estradiol-17β (OVX+low E2) receiving 2-deoxy-D-glucose (2DG), an inhibitor of glucose utilization, intravenously (iv) were used as a malnutrition model. An administration of D-Phe-Cys-Tyr-D-Trp-Orn-Thr-Pen-Thr-NH2 (CTOP), a selective MOR antagonist, into the third ventricle blocked the suppression of the LH pulse and increase in gluconeogenesis/feeding induced by iv 2DG administration. Histological analysis revealed that arcuate Kiss1 (kisspeptin gene)-expressing cells and preoptic Gnrh1 (GnRH gene)-expressing cells co-expressed little Oprm1 (MOR gene), while around 10% of arcuate Slc17a6 (glutamatergic marker gene)-expressing cells co-expressed Oprm1. Further, the CTOP treatment decreased the number of fos-positive cells in the paraventricular nucleus (PVN) in OVX+low E2 rats treated with iv 2DG but failed to affect the number of arcuate fos-expressing Slc17a6-positive cells. Taken together, these results suggest that the central β-endorphin-MOR signaling mediates the suppression of pulsatile LH release and that the β-endorphin may indirectly suppress the arcuate kisspeptin neurons, a master regulator for GnRH/LH pulses during malnutrition. Furthermore, the current study suggests that central β-endorphin-MOR signaling is also involved in gluconeogenesis and an increase in food intake by directly or indirectly acting on the PVN neurons during malnutrition in female rats.

    DOI: 10.1210/endocr/bqab140

    Web of Science

    Scopus

    PubMed

  5. Kisspeptin Neurons and Estrogen-Estrogen Receptor alpha Signaling: Unraveling the Mystery of Steroid Feedback System Regulating Mammalian Reproduction

    Uenoyama Yoshihisa, Inoue Naoko, Nakamura Sho, Tsukamura Hiroko

    INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES   Vol. 22 ( 17 )   2021.9

     More details

    Language:Japanese   Publisher:International Journal of Molecular Sciences  

    Estrogen produced by ovarian follicles plays a key role in the central mechanisms control-ling reproduction via regulation of gonadotropin‐releasing hormone (GnRH) release by its negative and positive feedback actions in female mammals. It has been well accepted that estrogen receptor α (ERα) mediates both estrogen feedback actions, but precise targets had remained as a mystery for decades. Ever since the discovery of kisspeptin neurons as afferent ERα‐expressing neurons to gov-ern GnRH neurons, the mechanisms mediating estrogen feedback are gradually being unraveled. The present article overviews the role of kisspeptin neurons in the arcuate nucleus (ARC), which are considered to drive pulsatile GnRH/gonadotropin release and folliculogenesis, in mediating the estrogen negative feedback action, and the role of kisspeptin neurons located in the anteroventral periventricular nucleus‐periventricular nucleus (AVPV‐PeN), which are thought to drive GnRH/lu-teinizing hormone (LH) surge and consequent ovulation, in mediating the estrogen positive feedback action. This implication has been confirmed by the studies showing that estrogen‐bound ERα down‐ and up‐regulates kisspeptin gene (Kiss1) expression in the ARC and AVPV‐PeN kisspeptin neurons, respectively. The article also provides the molecular and epigenetic mechanisms regulating Kiss1 expression in kisspeptin neurons by estrogen. Further, afferent ERα‐expressing neurons that may regulate kisspeptin release are discussed.

    DOI: 10.3390/ijms22179229

    Web of Science

    Scopus

    PubMed

  6. Morphological Analysis of the Hindbrain Glucose Sensor-Hypothalamic Neural Pathway Activated by Hindbrain Glucoprivation

    Sato Marimo, Minabe Shiori, Sakono Takahiro, Magata Fumie, Nakamura Sho, Watanabe Youki, Inoue Naoko, Uenoyama Yoshihisa, Tsukamura Hiroko, Matsuda Fuko

    ENDOCRINOLOGY   Vol. 162 ( 9 )   2021.9

     More details

    Language:Japanese   Publisher:Endocrinology (United States)  

    Lowered glucose availability, sensed by the hindbrain, has been suggested to enhance gluconeogenesis and food intake as well as suppress reproductive function. In fact, our previous histological and in vitro studies suggest that hindbrain ependymal cells function as a glucose sensor. The present study aimed to clarify the hindbrain glucose sensor-hypothalamic neural pathway activated in response to hindbrain glucoprivation to mediate counterregulatory physiological responses. Administration of 2-deoxy-D-glucose (2DG), an inhibitor of glucose utilization, into the fourth ventricle (4V) of male rats for 0.5 hour induced messenger RNA (mRNA) expression of c-fos, a marker for cellular activation, in ependymal cells in the 4V, but not in the lateral ventricle, the third ventricle or the central canal without a significant change in blood glucose and testosterone levels. Administration of 2DG into the 4V for 1 hour significantly increased blood glucose levels, food intake, and decreased blood testosterone levels. Simultaneously, the expression of c-Fos protein was detected in the 4V ependymal cells; dopamine β-hydroxylase-immunoreactive cells in the C1, C2, and A6 regions; neuropeptideY (NPY) mRNA-positive cells in the C2; corticotropin-releasing hormone (CRH) mRNA-positive cells in the hypothalamic paraventricular nucleus (PVN); and NPY mRNA-positive cells in the arcuate nucleus (ARC).Taken together, these results suggest that lowered glucose availability, sensed by 4V ependymal cells, activates hindbrain catecholaminergic and/or NPY neurons followed by CRH neurons in the PVN and NPY neurons in the ARC, thereby leading to counterregulatory responses, such as an enhancement of gluconeogenesis, increased food intake, and suppression of sex steroid secretion.

    DOI: 10.1210/endocr/bqab125

    Web of Science

    Scopus

    PubMed

  7. Paraventricular dynorphin A neurons mediate the suppression of LH pulse in lactating rats

    TSUCHIDA Hitomi, INOUE Naoko, UENOYAMA Yoshihisa, TSUKAMURA Hiroko

    The Journal of Reproduction and Development Supplement   Vol. 114 ( 0 ) page: OR-26 - OR-26   2021

     More details

    Language:Japanese   Publisher:The Society for Reproduction and Development  

    DOI: 10.14882/jrds.114.0_or-26

    CiNii Research

  8. Expression of estrogen receptor co-repressor gene in <i>Kiss1-</i> or <i>Tac3-</i>expressing cells in lactating rats

    TAKIZAWA Marina, UENOYAMA Yoshihisa, INOUE Naoko, TSUKAMURA Hiroko

    The Journal of Reproduction and Development Supplement   Vol. 114 ( 0 ) page: OR-25 - OR-25   2021

     More details

    Language:Japanese   Publisher:The Society for Reproduction and Development  

    DOI: 10.14882/jrds.114.0_or-25

    CiNii Research

  9. Conditional kisspeptin neuron-specific Kiss1 knockout with newly generated Kiss1-floxed and Kiss1-Cre mice replicates a hypogonadal phenotype of global Kissl knockout mice

    Ikegami Kana, Goto Teppei, Nakamura Sho, Watanabe Youki, Sugimoto Arisa, Majarune Sutisa, Horihata Kei, Nagae Mayuko, Tomikawa Junko, Imamura Takuya, Sanbo Makoto, Hirabayashi Masumi, Inoue Naoko, Maeda Kei-ichiro, Tsukamura Hiroko, Uenoyama Yoshihisa

    JOURNAL OF REPRODUCTION AND DEVELOPMENT   Vol. 66 ( 4 ) page: 359 - 367   2020.8

     More details

    Language:Japanese  

    Web of Science

  10. Expression of estrogen receptor α corepressor(s) in kisspeptin neurons in female rats

    MIYAZAKI Sae, INOUE Naoko, TSUKAMURA Hiroko, YOSHIHISA Uenoyama

    The Journal of Reproduction and Development Supplement   Vol. 113 ( 0 ) page: P-5 - P-5   2020

     More details

    Language:Japanese   Publisher:The Society for Reproduction and Development  

    DOI: 10.14882/jrds.113.0_p-5

    CiNii Research

  11. Direct evidence that KNDy neurons maintain gonadotropin pulses and folliculogenesis as the GnRH pulse generator. Reviewed

    Nagae M, Uenoyama Y, Okamoto S, Tsuchida H, Ikegami K, Goto T, Majarune S, Nakamura S, Sanbo M, Hirabayashi M, Kobayashi K, Inoue N, Tsukamura H

    Proceedings of the National Academy of Sciences of the United States of America   Vol. 118 ( 5 )   2021.2

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1073/pnas.2009156118

    PubMed

  12. Mating-induced increase in Kiss1 mRNA expression in the anteroventral periventricular nucleus prior to an increase in LH and testosterone release in male rats. Reviewed

    Watanabe Y, Ikegami K, Nakamura S, Uenoyama Y, Ozawa H, Maeda KI, Tsukamura H, Inoue N

    The Journal of reproduction and development   Vol. 66 ( 6 ) page: 579 - 586   2020.12

     More details

    Authorship:Last author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1262/jrd.2020-067

    PubMed

  13. Seasonal changes in the reproductive performance in local cows receiving artificial insemination in the Pursat province of Cambodia Invited International coauthorship

    Tep Bengthay, Morita Yasuhiro, Matsuyama Shuichi, Ohkura Satoshi, Inoue Naoko, Tsukamura Hiroko, Uenoyama Yoshihisa, Pheng Vutha

    ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES   Vol. 33 ( 12 ) page: 1922 - 1929   2020.12

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.5713/ajas.19.0893

    Web of Science

    PubMed

  14. Paraventricular Dynorphin A Neurons Mediate LH Pulse Suppression Induced by Hindbrain Glucoprivation in Female Rats Reviewed

    Tsuchida Hitomi, Mostari Parvin, Yamada Koki, Miyazaki Sae, Enomoto Yuki, Inoue Naoko, Uenoyama Yoshihisa, Tsukamura Hiroko

    ENDOCRINOLOGY   Vol. 161 ( 11 )   2020.11

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1210/endocr/bqaa161

    Web of Science

    PubMed

  15. Facilitatory and inhibitory role of central amylin administration in the regulation of the gonadotropin-releasing hormone pulse generator activity in goats Reviewed

    Kitagawa Yuri, Sasaki Takuya, Suzumura Reika, Morishima Ai, Tatebayashi Ryoki, Assadullah, Ieda Nahoko, Morita Yasuhiro, Matsuyama Shuichi, Inoue Naoko, Uenoyama Yoshihisa, Tsukamura Hiroko, Ohkura Satoshi

    NEUROSCIENCE LETTERS   Vol. 736   page: 135276   2020.9

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.neulet.2020.135276

    Web of Science

    PubMed

  16. Testosterone Supplementation Rescues Spermatogenesis and In Vitro Fertilizing Ability of Sperm in Kiss1 Knockout Mice Reviewed

    Goto Teppei, Hirabayashi Masumi, Watanabe Youki, Sanbo Makoto, Tomita Koichi, Inoue Naoko, Tsukamura Hiroko, Uenoyama Yoshihisa

    ENDOCRINOLOGY   Vol. 161 ( 9 )   2020.9

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1210/endocr/bqaa092

    Web of Science

    PubMed

  17. Conditional kisspeptin neuron-specific Kiss1 knockout with newly generated Kiss1-floxed and Kiss1-Cre mice replicates a hypogonadal phenotype of global Kiss1 knockout mice. Reviewed

    Ikegami K, Goto T, Nakamura S, Watanabe Y, Sugimoto A, Majarune S, Horihata K, Nagae M, Tomikawa J, Imamura T, Sanbo M, Hirabayashi M, Inoue N, Maeda KI, Tsukamura H, Uenoyama Y

    The Journal of reproduction and development   Vol. 66 ( 4 ) page: 359 - 367   2020.8

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1262/jrd.2020-026

    PubMed

  18. Inducible Kiss1 knockdown in the hypothalamic arcuate nucleus suppressed pulsatile secretion of luteinizing hormone in male mice Reviewed

    Minabe Shiori, Nakamura Sho, Fukushima Eri, Sato Marimo, Ikegami Kana, Goto Teppei, Sanbo Makoto, Hirabayashi Masumi, Tomikawa Junko, Imamura Takuya, Inoue Naoko, Uenoyama Yoshihisa, Tsukamura Hiroko, Maeda Kei-Ichiro, Matsuda Fuko

    JOURNAL OF REPRODUCTION AND DEVELOPMENT   Vol. 66 ( 4 ) page: 369 - 375   2020.8

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    Web of Science

  19. Establishment of immortalised cell lines derived from female Shiba goat KNDy and GnRH neurones Reviewed

    Suetomi Yuta, Tatebayashi Ryoki, Sonoda Shuhei, Munetomo Arisa, Matsuyama Shuichi, Inoue Naoko, Uenoyama Yoshihisa, Takeuchi Yukari, Tsukamura Hiroko, Ohkura Satoshi, Matsuda Fuko

    JOURNAL OF NEUROENDOCRINOLOGY   Vol. 32 ( 6 ) page: e12857   2020.6

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1111/jne.12857

    Web of Science

    PubMed

  20. Peripheral administration of SB223412, a selective neurokinin-3 receptor antagonist, suppresses pulsatile luteinizing hormone secretion by acting on the gonadotropin-releasing hormone pulse generator in estrogen-treated ovariectomized female goats. Reviewed

    Sasaki T, Sonoda T, Tatebayashi R, Kitagawa Y, Oishi S, Yamamoto K, Fujii N, Inoue N, Uenoyama Y, Tsukamura H, Maeda KI, Matsuda F, Morita Y, Matsuyama S, Ohkura S

    The Journal of reproduction and development     2020.4

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  21. GnRH(1-5), a metabolite of gonadotropin-releasing hormone, enhances luteinizing hormone release via activation of kisspeptin neurons in female rats Reviewed

    Ieda Nahoko, Assadullah, Minabe Shiori, Ikegami Kana, Watanabe Youki, Sugimoto Yusuke, Sugimoto Arisa, Kawai Narumi, Ishii Hirotaka, Inoue Naoko, Uenoyama Yoshihisa, Tsukamura Hiroko

    ENDOCRINE JOURNAL   Vol. 67 ( 4 ) page: 409 - 418   2020

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1507/endocrj.EJ19-0444

    Web of Science

    PubMed

  22. Analysis of the involvement of serotonin receptor subtype in the regulation of GnRH pulse generator activity in Shiba goats

    SUZUMURA Reika, MORITA Yasuhiro, MATSUYAMA Shuichi, SASAKI Takuya, KITAGAWA Yuri, INOUE Naoko, UENOYAMA Yoshihisa, TSUKAMURA Hiroko, OHKURA Satoshi

    The Journal of Reproduction and Development Supplement   Vol. 113 ( 0 ) page: P - 4-P-4   2020

     More details

    Publisher:THE SOCIETY FOR REPRODUCTION AND DEVELOPMENT  

    DOI: 10.14882/jrds.113.0_P-4

  23. Role of kisspeptin neurons as a GnRH surge generator: Comparative aspects in rodents and non-rodent mammals Reviewed

    Matsuda Fuko, Ohkura Satoshi, Magata Fumie, Munetomo Arisa, Chen Jing, Sato Marimo, Inoue Naoko, Uenoyama Yoshihisa, Tsukamura Hiroko

    JOURNAL OF OBSTETRICS AND GYNAECOLOGY RESEARCH   Vol. 45 ( 12 ) page: 2318 - 2329   2019.12

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1111/jog.14124

    Web of Science

    PubMed

  24. Ad libitum feeding triggers puberty onset associated with increases in arcuate Kiss1 and Pdyn expression in growth-retarded rats Reviewed

    Majarune Sutisa, Nima Pelden, Sugimoto Arisa, Nagae Mayuko, Inoue Naoko, Tsukamura Hiroko, Uenoyama Yoshihisa

    JOURNAL OF REPRODUCTION AND DEVELOPMENT   Vol. 65 ( 5 ) page: 397 - 406   2019.10

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    Web of Science

  25. Central Mechanism Controlling Pubertal Onset in Mammals: A Triggering Role of Kisspeptin

    Uenoyama Yoshihisa, Inoue Naoko, Nakamura Sho, Tsukamura Hiroko

    FRONTIERS IN ENDOCRINOLOGY   Vol. 10   page: 312   2019.5

  26. Neonatal Estrogen Causes Irreversible Male Infertility via Specific Suppressive Action on Hypothalamic Kiss1 Neurons

    Minabe Shiori, Sato Marimo, Inoue Naoko, Watanabe Youki, Magata Fumie, Matsuda Fuko, Uenoyama Yoshihisa, Ozawa Hitoshi, Tsukamura Hiroko

    ENDOCRINOLOGY   Vol. 160 ( 5 ) page: 1223 - 1233   2019.5

  27. Somatostatin-Somatostatin Receptor 2 Signaling Mediates LH Pulse Suppression in Lactating Rats

    Sugimoto Arisa, Tsuchida Hitomi, Ieda Nahoko, Ikegami Kana, Inoue Naoko, Uenoyama Yoshihisa, Tsukamura Hiroko

    ENDOCRINOLOGY   Vol. 160 ( 2 ) page: 473-483   2019.2

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1210/en.2018-00882

    Web of Science

    PubMed

  28. Morphological analysis for neuronal pathway from the hindbrain ependymocytes to the hypothalamic kisspeptin neurons.

    Deura C, Minabe S, Ikegami K, Inoue N, Uenoyama Y, Maeda KI, Tsukamura H

    The Journal of reproduction and development     2019.1

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1262/jrd.2018-122

    PubMed

  29. Effects of estradiol on <i>Rbbp7 </i>expression in <i>Kiss1</i>-expressing cells

    HORIHATA Kei, INOUE Naoko, UENOYAMA Yoshihisa, MAEDA Kei-Ichiro, TSUKAMURA Hiroko

    The Journal of Reproduction and Development Supplement   Vol. 112 ( 0 ) page: P - 4-P-4   2019

     More details

    Publisher:THE SOCIETY FOR REPRODUCTION AND DEVELOPMENT  

    DOI: 10.14882/jrds.112.0_P-4

  30. Identification of GnRH pulse generator by analyses of two-modes of LH secretion in arcuate-specific <i>Kiss1 </i>knockout rats

    NAGAE Mayuko, GOTO Teppei, YOGO Kyoko, SANBO Makoto, HIRABAYASHI Masumi, KOBAYASHI Kenta, INOUE Naoko, TSUKAMURA Hiroko, UENOYAMA Yoshihisa

    The Journal of Reproduction and Development Supplement   Vol. 112 ( 0 ) page: AW1 - 7-AW1-7   2019

     More details

    Publisher:THE SOCIETY FOR REPRODUCTION AND DEVELOPMENT  

    DOI: 10.14882/jrds.112.0_AW1-7

  31. Paraventricular dynorphin A neurons mediate the glucoprivic suppression of luteinizing hormone release in female rats

    TSUCHIDA Hitomi, INOUE Naoko, UENOYAMA Yoshihisa, TSUKAMURA Hiroko

    The Journal of Reproduction and Development Supplement   Vol. 112 ( 0 ) page: OR2 - 2-OR2-2   2019

     More details

    Publisher:THE SOCIETY FOR REPRODUCTION AND DEVELOPMENT  

    DOI: 10.14882/jrds.112.0_OR2-2

  32. The role of calcitonin receptor in the regulation of pulsatile GnRH secretion

    YURI Kitagawa, OHKURA Satoshi, SASAKI Takuya, MORISHIMA Ai, TATEBAYASHI Ryoki, MORITA Yasuhiro, MATSUYAMA Shuichi, INOUE Naoko, UENOYAMA Yoshihisa, TSUKAMURA Hiroko

    The Journal of Reproduction and Development Supplement   Vol. 112 ( 0 ) page: P - 6-P-6   2019

     More details

    Publisher:THE SOCIETY FOR REPRODUCTION AND DEVELOPMENT  

    DOI: 10.14882/jrds.112.0_P-6

  33. The role of serotonin in the regulation of the GnRH pulse generator activity in goats

    SASAKI Takuya, UENOYAMA Yoshihisa, TSUKAMURA Hiroko, OHKURA Satoshi, MORISHIMA Ai, NAKANISHI Marina, SUZUMURA Reika, TATEBAYASHI Ryoki, KITAGAWA Yuri, MORITA Yasuhiro, MATSUYAMA Shuichi, INOUE Naoko

    The Journal of Reproduction and Development Supplement   Vol. 112 ( 0 ) page: OR2 - 1-OR2-1   2019

     More details

    Publisher:THE SOCIETY FOR REPRODUCTION AND DEVELOPMENT  

    DOI: 10.14882/jrds.112.0_OR2-1

  34. Verification of suppressive effect of subcutaneous NK3R antagonist implant on reproductive function in male Japanese macaque

    SAKONO Takahiro, SUZUKI Juri, MATSUDA Fuko, CHEN Jing, MAGATA Fumie, SASAKI Takuya, YAMAMOTO Koki, OISHI Shinya, INOUE Naoko, UENOYAMA Yoshihisa, TSUKAMURA Hiroko

    The Journal of Reproduction and Development Supplement   Vol. 112 ( 0 ) page: P - 8-P-8   2019

     More details

    Publisher:THE SOCIETY FOR REPRODUCTION AND DEVELOPMENT  

    DOI: 10.14882/jrds.112.0_P-8

  35. The roles of kisspeptin in the mechanism underlying reproductive functions in mammals

    Uenoyama Yoshihisa, Inoue Naoko, Maeda Kei-ichiro, Tsukamura Hiroko

    JOURNAL OF REPRODUCTION AND DEVELOPMENT   Vol. 64 ( 6 ) page: 469-476   2018.12

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    Web of Science

  36. Co-expression of the calcitonin receptor gene in the hypothalamic kisspeptin neurons in female rats

    Assadullah, Ieda Nahoko, Kawai Narumi, Ishii Hirotaka, Ihara Kunio, Inoue Naoko, Uenoyama Yoshihisa, Tsukamura Hiroko

    REPRODUCTIVE MEDICINE AND BIOLOGY   Vol. 17 ( 2 ) page: 164 - 172   2018.4

  37. Evaluation of heat stress response in crossbred dairy cows under tropical climate by analysis of heart rate variability

    Bun Chan, Watanabe Youki, Uenoyama Yoshihisa, Inoue Naoko, Ieda Nahoko, Matsuda Fuko, Tsukamura Hiroko, Kuwahara Masayoshi, Maeda Kei-ichiro, Ohkura Satoshi, Pheng Vutha

    JOURNAL OF VETERINARY MEDICAL SCIENCE   Vol. 80 ( 1 ) page: 181-185   2018.1

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1292/jvms.17-0368

    Web of Science

    PubMed

  38. Increase in arcuate <i>Kiss1 </i>and <i>Pdyn </i>expressions may trigger the puberty onset in female rats

    MAJARUNE Sutisa, NIMA Pelden, INOUE Naoko, TSUKAMURA Hiroko, UENOYAMA Yoshihisa

    The Journal of Reproduction and Development Supplement   Vol. 111 ( 0 ) page: OR2 - 23-OR2-23   2018

     More details

    Publisher:THE SOCIETY FOR REPRODUCTION AND DEVELOPMENT  

    <p>The timing of puberty onset is largely dependent on body weight rather than chronological age. To clarify the mechanism underlying the puberty onset, the present study examined the effects of food restriction and subsequent relief from the restriction on <i>Kiss1 </i>(kisspeptin gene), <i>Tac3 </i>(neurokinin B gene) and <i>Pdyn </i>(Dynorphine A gene) expressions in the arcuate nucleus (ARC) and puberty onset in rats, because the ARC kisspeptin neurons are considered to be responsible for GnRH/gonadotropin pulse generation. Female rats weaned on postnatal day (PND) 20 were <i>ad lib</i>-fed by the day when their body weight (BW) reached 75 g, and they were subjected to a restricted feeding to keep their BW at 70–80 g by PND 49. Then, half of the animals were subjected to ad libitum feeding on PDN 49 for 24 h and 48 h. The growth-restricted rats never showed pubertal sign during the experimental period. On the other hand, <i>ad lib</i>-fed animals showed catch-up BW growth and the pubertal sign. Animals that underwent catch-up growth showed apparent LH pulses on PND 50 and a LH surge and vaginal proestrus on PND 51, whereas growth-restricted rats showed suppressed LH release and vaginal diestrus on both days. The numbers of ARC <i>Kiss1</i>- and <i>Pdyn</i>-expressing cells were significantly higher in the <i>ad lib</i>-fed rats than growth-restricted controls on PNDs 50 and 51. No significant difference was found in the number of <i>Tac3</i>-expressing cells between groups. These results suggest that increases in kisspeptin and dynorphin A expressions in the ARC might be required for function of GnRH/gonadotropin pulse generator and that the increase may trigger the puberty onset via an increase in pulsatile GnRH/gonadotropin release in female rats.</p>

    DOI: 10.14882/jrds.111.0_OR2-23

  39. Identification of gonadotropin-releasing hormone pulse generator by the generation of arcuate-specific <i>Kiss1</i> knockout rats

    NAGAE Mayuko, UENOYAMA Yoshihisa, GOTO Teppei, YOGO Kyoko, SANBO Makoto, HIRABAYASHI Masumi, KOBAYASHI Kenta, INOUE Naoko, TSUKAMURA Hiroko, MAEDA Kei-Ichiro

    The Journal of Reproduction and Development Supplement   Vol. 111 ( 0 ) page: OR2 - 25-OR2-25   2018

     More details

    Publisher:THE SOCIETY FOR REPRODUCTION AND DEVELOPMENT  

    DOI: 10.14882/jrds.111.0_OR2-25

  40. Long-Term Neonatal Estrogen Exposure Causes Irreversible Inhibition of LH Pulses by Suppressing Arcuate Kisspeptin Expression via Estrogen Receptors alpha and beta in Female Rodents

    Minabe Shiori, Ieda Nahoko, Watanabe Youki, Inoue Naoko, Uenoyama Yoshihisa, Maeda Kei-ichiro, Tsukamura Hiroko

    ENDOCRINOLOGY   Vol. 158 ( 9 ) page: 2918-2929   2017.9

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1210/en.2016-1144

    Web of Science

    PubMed

  41. Enhancement of the luteinising hormone surge by male olfactory signals is associated with anteroventral periventricular Kiss1 cell activation in female rats Reviewed

    Watanabe Y., Ikegami K., Ishigaki R., Ieda N., Uenoyama Y., Maeda K. I., Tsukamura H., Inoue N.

    JOURNAL OF NEUROENDOCRINOLOGY   Vol. 29 ( 8 )   2017.8

     More details

    Authorship:Last author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1111/jne.12505

    Web of Science

  42. Evidence of involvement of neurone-glia/neurone-neurone communications via gap junctions in synchronised activity of KNDy neurones

    Ikegami K., Minabe S., Ieda N., Goto T., Sugimoto A., Nakamura S., Inoue N., Oishi S., Maturana A. D., Sanbo M., Hirabayashi M., Maeda K-I, Tsukamura H., Uenoyama Y.

    JOURNAL OF NEUROENDOCRINOLOGY   Vol. 29 ( 6 )   2017.6

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1111/jne.12480

    Web of Science

  43. 視床下部による卵胞発育制御機構

    井上直子、束村博子、上野山賀久

    臨床婦人科産科   Vol. 70 ( 12 ) page: 1094-1097   2016.12

     More details

    Authorship:Lead author   Language:Japanese  

  44. Pharmacological and morphological evidence of AMPK-mediated energy sensing in the lower brain stem ependymocytes to control reproduction in female rodents. Reviewed

    Minabe S, Deura C, Ikegami K, Goto T, Sanbo M, Hirabayashi M, Inoue N, Uenoyama Y, Maeda KI, Tsukamura H.

    Endocrinology   Vol. 156   page: 2267-2278   2015

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  45. The LH surge-generating system is functional in male goats as in females: Involvement of kisspeptin neurones in the medial preoptic area. Reviewed

    Matsuda F, Nakatsukasa K, Suetomi Y, Naniwa Y, Ito D, Inoue N, Wakabayashi Y, Okamura H, Maeda KI, Uenoyama Y, Tsukamura H, Ohkura S.

    J Neuroendocrinol   Vol. 27   page: 187-197   2015

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  46. Oestrogen-induced activation of preoptic kisspeptin neurones may be involved in the luteinizing hormone surge in male and female Japanese monkeys. Reviewed

      Vol. 27   page: 57-65   2014

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  47. KISS1 gene expression in the developing brain of female pigs in pre- and peripubertal periods. Reviewed

    J Reprod Dev   Vol. 60   page: 312-316   2014

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  48. The contribution of leukemia inhibitory factor (LIF) for embryo implantation differs among strains of mice. Reviewed

    Kobayashi R, Terakawa J, Kato Y, Azimi S, Inoue N, Ohmori Y, Hondo E.

    Immunobiology   Vol. 219   page: 512-521   2014

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  49. Epigenetic regulation of Kiss1 gene expression mediating estrogen-positive feedback action in the mouse brain Reviewed

    Tomikawa J, Uenoyama Y, Ozawa M, Fukanuma T, Takase K, Goto T, Abe H, Ieda N, Minabe S, Deura C, Inoue N, Sanbo M, Tomita K, Hirabayashi M, Tanaka S, Imamura T, Okamura H, Maeda K, Tsukamura H.

    Proc Natl Acad Sci U S A.   Vol. 109   page: E1294-1301   2012.5

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  50. The complete control of murine pregnancy from embryo implantation to parturition. Reviewed

    Terakawa J, Watanabe T, Obara R, Sugiyama M, Inoue N, Ohmori Y, Hosaka YZ, Hondo E.

    Reproduction   Vol. 143 ( 3 ) page: 411-415   2012

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  51. キスペプチンと生殖腺刺激ホルモン放出ホルモン(GnRH)分泌

    井上直子、束村博子、前多敬一郎

    Clinical Neuroscience   Vol. 30 ( 2 ) page: 220-222   2012

     More details

    Authorship:Lead author   Language:Japanese  

  52. 交尾が排卵を引き起こすしくみ

    井上直子

    名大トピックス   ( 231 ) page: 8-9   2012

     More details

    Authorship:Lead author   Language:Japanese  

  53. Embryo implantation is blocked by intraperitoneal injection with anti-LIF antibody in mice. Reviewed

    Terakawa J, Wakitani S, Sugiyama M, Inoue N, Ohmori Y, Kiso Y, Hosaka YZ, Hondo E.

    J Reprod Dev.   Vol. 57 ( 6 ) page: 700-707   2011.12

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  54. Kisspeptin neurons mediate reflex ovulation in the musk shrew (Suncus murinus).

    Inoue, N. Sasagawa, K. Ikai, K. Sasaki, Y. Tomikawa, J. Oishi, S. Fujii, N. Uenoyama, Y. Ohmori, Y. Yamamoto, N. Hondo, E. Maeda, KI. Tsukamura, H.

    Proc Natl Acad Sci U S A.   Vol. 108   page: 17527-17532   2011.10

     More details

    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

  55. Ultrastructural Evidence of Kisspeptin-Gonadotrophin-Releasing Hormone Interaction in the Median Eminence of Female Rats: Implication of Axo-Axonal Regulation of GnRH Release. Reviewed

    Uenoyama Y*, Inoue N*, Pheng V, Homma T, Takase K, Yamada S, Ajiki K, Ichikawa M, Okamura H, Maeda KI, Tsukamura H. (*: equal contributed)

    J Neuroendocrinol   Vol. 23   page: 863-870   2011.7

     More details

    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

  56. Role of cell-death ligand-receptor system of granulosa cells in selective follicular atresia in porcine ovary. Invited Reviewed

      Vol. 57   page: 169-175   2011

     More details

    Authorship:Lead author   Language:English  

  57. Expression and function of apoptosis inhibitor FOXO3 in guranulosa cells during follicular atresia Reviewed

      Vol. 57   page: 151-158   2011

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  58. 卵胞発育の中枢制御機構

    井上直子、上野山賀久、大蔵聡、束村博子、前多敬一郎

    Hormone Frontier in Gynecology   Vol. 18 ( 4 ) page: 391-394   2011

     More details

    Authorship:Lead author   Language:Japanese  

  59. キスペプチン/メタスチン-繁殖を制御する新規神経ペプチド

    大蔵聡、上野山賀久、冨川順子、井上直子、束村博子、前多敬一郎

    日本獣医師学会雑誌   Vol. 64 ( 1 ) page: 39-44   2011

     More details

    Language:Japanese  

  60. キスペプチンによる繁殖機能制御機構

    上野山賀久、大蔵聡、井上直子、前多敬一郎、束村博子

    臨床科学   Vol. 40 ( 2 ) page: 113-118   2011

     More details

    Language:Japanese  

  61. Molecular characterization and estrogen regulation of hypothalamic KISS1 gene in pig. Reviewed

    Tomikawa J, Homma T, Tajima S, Shibata T, Inamoto Y, Takase K, Inoue N, Ohkura S, Uenoyama Y, Maeda K, Tsukamura H.

    Biol Reprod   Vol. 82   page: 313-319   2010

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  62. Activation of Neuropeptide FF Receptors by Kisspeptin Receptor ligands. Reviewed

    Oishi S, Misu R, Tomita K, Setuda S, Masuda R, Ohno H, Naniwa Y, Ieda N, Inoue N, Ohkura S, Uenoyama Y, Tsukamura H, Maeda K-I, Hirasawa A, Tsujimoto G, Fujii N.

    ACS Medicinal Chemistry letters   Vol. 2 ( 1 ) page: 53-57   2010

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1021/ml1002053

  63. Movement of lyle's flying fox (pteropus lylei) in central thailand.

    Hondo E, Inoue N, Maeda K, Rerkamnuaychoke W, Duengkae P.

    Journal of wildlife in Thailand   Vol. 17 ( 1 ) page: 55-63   2010

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  64. Physiological role of metastin/kisspeptin in regulating gonadotropin-releasing hormone (GnRH) secretion in female rats. Reviewed

    Ohkura S, Uenoyama Y, Yamada S, Homma T, Takase K, Inoue N, Maeda KI, Tsukamura H.

    Peptides   Vol. 30   page: 49-56   2009

     More details

    Language:English  

  65. Possible role of oestrogen in pubertal increase of Kiss1/kisspeptin expression in discrete hypothalamic areas of female rats. Reviewed

    Takase K, Uenoyama Y, Inoue N, Matsui H, Yamada S, Shimizu M, Homma T, Tomikawa J, Kanda S, Matsumoto H, Oka Y, Tsukamura H, Maeda KI.

    J Neuroendocrinol   Vol. 21   page: 527-537   2009

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  66. cFLIP Regulates Death Receptor-mediated Apoptosis in an Ovarian Granulosa Cell Line by Inhibiting Procaspase-8 Cleavage. Reviewed

    Journal of Reproduction and Development   Vol. 54   page: 314-20   2008

     More details

    Language:English  

  67. Molecular cloning of a porcine (Sus scrofa) apoptosis inhibitory ligand, netrin-1, and its receptor, p53RDL1. Reviewed

    Maeda A, Matsuda F, Goto Y, Cheng Y, Gonda H, Inoue N, Nakagawa S, Manabe N.

    Journal of Reproduction and Development   Vol. 54   page: 275-280   2008

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  68. Changes in Expression and Localization of X-linked Inhibitor of Apoptosis Protein (XIAP) in Follicular Granulosa Cells During Atresia in Porcine Ovaries. Reviewed

    Yuan Cheng, Naoko Inoue, Fuko Matsuda-Minehata, Yasufumi Goto, Akihisa Maeda, Noboru Manabe.

    Journal of Reproduction and Development   Vol. 54   page: 454-459   2008

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  69. Role of MAdCAM-1 and its ligand on the homing of transplanted hematopoietic cells in irradiated mice. Reviewed

    Tada T, Inoue N, Widayati DT, Fukuta K.

    Exp Anim   Vol. 57   page: 347-356   2008

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  70. 視床下部-下垂体-性腺軸のマスターレギュレーターとしてのメタスチン(キスペプチン)

    束村博子、上野山賀久、山田俊児、冨川順子、井上直子、大蔵聡、前多敬一郎

    ホルモンと臨床   Vol. 56 ( 8 ) page: 771-777   2008

     More details

    Language:Japanese  

  71. The Role of Interleukin-6 in the Regulation of Granulosa Cell Apoptosis during Follicular Atresia in Pig Ovaries. Reviewed

    Maeda A, Inoue N, Matsuda-Minehata F, Goto Y, Cheng Y, Manabe N.

    Journal of Reproduction and Development   Vol. 53   2007

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  72. Molecular Characteristics of Porcine Fas-associated Death Domain (FADD) and Procaspase-8 Reviewed

    Inoue N, Matsuda-Minehata F, Goto Y, Sakamaki K, Manabe N

    Journal of Reproduction and Devevelopment   Vol. 53   page: 427-436   2007

     More details

    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

    To reveal the intracellular signal transduction molecules in granulosa cell apoptosis in porcine ovarian follicles, I cloned the porcine Fas-associated death domain (FADD), an adaptor protein for the cell death receptor, and procaspase-8, an initiator caspase. Porcine FADD (pFADD) was 636 bp (211 amino acids: aa) long and showed 74.0 and 65.4% homology with human and murine FADD, respectively. Porcine procaspase-8 (pprocaspase-8) was 1,431 bp (476 aa) long and 70.6 and 63.4% homologous with human and murine procaspase-8, respectively. To confirm the apoptosis-inducing abilities, I constructed pFADD- and pprocaspase-8-cDNA expression vectors with enhanced green fluorescence protein (EGFP), and then transfected them into human uterine cervix tumor (HeLa-K), human granulosa-cell derived (KGN), murine granulosa-derived tumor (KK1), and porcine granulosa-derived (JC410) cells. When pFADD and pprocaspase-8 were over-expressed, cell death was induced in these transfected cells. But when the caspase-inhibitor p35 was co-transfected, cell death was inhibited. The pFADD and pprocaspase-8 genes are well conserved, as are the physiological functions of their products.

  73. Anti-apoptotic activity of porcine cFLIP in ovarian granulosa cell lines. Reviewed

    Matsuda-Minehata F, Goto Y, Inoue N, Sakamaki K, Chedrese PJ, Manabe N.

    Mol Reprod Dev.   Vol. 74   page: 1165-1170   2007

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  74. Changes in expression of interleukin-6 receptors in granulosa cells during follicular atresia in pig ovaries. Reviewed

    Akihisa Maeda, Yasufumi Goto, Fuko Matsuda-Minehata, Yuan Cheng, Naoko Inoue, Noboru Manabe.

    J Reprod Dev   Vol. 53   page: 727-736   2007

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  75. *Changes in expression levels and localization of Fas ligand and Fas during atresia in porcine ovarian follicles. Reviewed

    Naoko Inoue, Akihisa Maeda, Fuko Matsuda-Minehata, Katsuhiro Fukuta, Noboru Manabe.

    Journal of Reproduction and Devevelopment   Vol. 52   2006

     More details

    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

    To reveal the mechanisms regulating the selective atresia of follicles in porcine ovaries, I examined the changes in the mRNA and protein levels of cell-death ligand, Fas/APO-1/CD95 ligand (FasL), and its receptor, Fas/APO-1/CD95 (Fas), and the localization of the proteins in granulosa cells during follicular atresia using the reverse transcription-polymerase chain reaction (RT-PCR), Western blotting, and immunohistochemical techniques, respectively. Trace levels of FasL mRNA and protein were detected in the granulosa cells of healthy follicles; however, weak levels were detected in those of early atretic follicles, and the levels increased during atresia. Trace/weak levels of Fas mRNA and protein were detected in the granulosa cells of healthy follicles. Fas protein was located in the cytoplasmic area, not in cell membrane area, indicating that it has no activity in regard to inducing apoptosis. When apoptosis commences in granulosa cells, Fas moves from the cytoplasmic to cell membrane area. FasL and Fas mRNAs and proteins in granulosa cells were upregulated during follicular atresia. The FasL and Fas system may play a crucial role in the regulation of apoptosis in granulosa cells during selective follicular atresia in porcine ovaries.

  76. Changes in expression of anti-apoptotic protein, cFLIP, in granulosa cells during follicular atresia in porcine ovaries. Reviewed

    Fuko Matsuda-Minehata, Yasufumi Goto, Naoko Inoue, Noboru Manabe.

    Molecular Reproduction and Development   Vol. 72   page: 145-151   2005

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  77. Changes in expression and localization of connexin 43 mRNA and protein in porcine ovary granulosa cells during follicular atresia. Reviewed

    Yuan Cheng, Naoko Inoue, Fuko Matsuda-Minehata, Yasufumi Goto, Akihisa Maeda, Noboru Manabe.

    Journal of Reproduction and Devevelopment   Vol. 51   page: 627-637   2005

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  78. Regulation mechanism of selective atresia in porcine follicles: regulation of granulosa cell apoptosis during atresia. Reviewed

    Noboru Manabe, Yasufumi Goto, Fuko Matsuda-Minehata, Naoko Inoue, Akihisa Maeda, Miki Sugimoto, Kazuhiro Sakamaki, Takashi Miyano

    Journal of Reproduction and Devevelopment   Vol. 50   page: 493-514   2004

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  79. The porcine (Sus scrofa) cellular Flice-like inhibitory protein (cFLIP): molecular cloning and comparison with the human and murine cFLIP Reviewed

    Yasufumi Goto, Fuko Matsuda, Toshikatsu Matsui, Akihisa Maeda, Naoko Inoue, Noboru Manabe.

    Journal of Reproduction and Devevelopment   Vol. 50   page: 549-555   2004

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  80. Changes in the expression and activity of caspase-9 and Apaf1 in granulosa cells during follicular atresia in pig ovaries. Reviewed

    Toshikatsu Matsui, Noboru Manabe, Yasufumi Goto, Mizuho Nakayama, Naoko Inoue, Susumu Nishihara, Hajime Miyamoto

    Reproduction   Vol. 126   page: 113-120   2003

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  81. Roles of tumor necrosis factor-related Apoptosis-inducing ligand Signaling Pathway in Granulosa Cell Apoptosis during Atresia in Pig Ovaries Reviewed

    Naoko Inoue, Noboru Manabe, Satoko Wada, Mizuho Nakayama, Toshikatsu Matsui, Hajime Miyamoto

    Journal of Reproduction and Devevelopment   Vol. 49 ( 4 ) page: 313-321   2003

     More details

    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

  82. Changes in the expression of tumor necrosis factor (TNF) a, TNFa Receptor (TNFR) 2 and TNFR-associated factor 2 in granulosa cells during atresia in pig ovaries. Reviewed

    Mizuho Nakayama, Noboru Manabe, Naoko Inoue, Toshikatsu Matsui, Hajime Miyamoto.

    Biology of Reproduction   Vol. 126   page: 113-120   2003

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  83. Monoclonal antibody recognizes follicular granulosa cell antigens in porcine ovaries. Journal of Reproduction and Devevelopment Reviewed

    Chiemi Tajima, Noboru Manabe, Mizuho Nakayama, Naoko Inoue, Toshikatsu Matsui, Eiichi Hondo, Hajime Miyamoto Hajime.

    Journal of Reproduction and Devevelopment   Vol. 48   page: 567-572   2002

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  84. TRAIL-decoy receptor-1 disappears in granulosa cells of atretic follicles in porcine ovaries. Reviewed

    Satoko Wada, Noboru Manabe, Naoko Inoue, Mizuho Nakayama, Toshikatsu Matsui, Hajime Miyamoto

    Journal of Reproduction and Devevelopment   Vol. 2002   page: 167-173   2002

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  85. TRADD is involved in apoptosis induction in granulosa cells during atresia in pig ovaries. Reviewed

    Satoko Wada, Noboru Manabe, Naoko Inoue, Mizuho Nakayama, Toshikatsu Matsui, Hajime Miyamoto.

    Journal of Reproduction and Devevelopment   Vol. 48   page: 175-181   2002

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  86. TRAIL-decoy receptor-1 plays inhibitory role in apoptosis of granulosa cells from pig ovarian follicules. Reviewed

    Satoko Wada, Noboru Manabe, Mizuho Nakayama, Naoko Inoue, Toshikatsu Matsui, Hajime Miyamoto

    The Journal of Veterinary Medical Science   Vol. 64 ( 5 ) page: 435-439   2002

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  87. Changes in Cell Cycle of Tetraploid Murine Embryos Induced by Cytochalasin B Reviewed

    Naoko Inoue, Akihiro Kiya, Noboru Manabe ,Hajime Miyamoto Katsuhiro Fukuta

      Vol. 23 ( 2 ) page: 44-45   2001

     More details

    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

  88. Changes in cell adhesion molecules during follicular atresia in porcine ovaries. Reviewed

    Susumu Nishihara, Noboru Manabe, Mizuho Nakayama, Satoko Wada, Naoko Inoue, Hajime Miyamoto.

    J Reprod Dev.   Vol. 46   page: 325-334   2000

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

▼display all

Books 2

  1. The Ovary (2nd edition)

    Noboru Manabe, Naoko Inoue, Takashi Miyano, Kazuhiro Sakamaki, Miki Sugimoto, Hajime Miyamoto.( Role: Joint author)

    Academic Press  2003 

     More details

    Language:English

  2. スンクスの生物学 Ⅵ−6 スンクスの卵巣内アポトーシスとステロイドホルモン合成酵素

    井上直子( Role: Sole author)

    学会出版センター  2011.2 

     More details

    Language:Japanese

Presentations 30

  1. 哺乳類の卵胞発育を制御する脳内メカニズムの解明

    長江麻佑子、土田仁美、井上直子、束村博子、上野山賀久

    令和3年度日本学術振興会育志賞研究発表会  2022.3.2  日本学術振興会

     More details

    Event date: 2022.3

    Language:Japanese   Presentation type:Poster presentation  

    Venue:WEB開催   Country:Japan  

  2. 室傍核ダイノルフィンニューロンは泌乳ラットにおけるLHパルスの抑制を仲介する

    土田仁美, 井上直子, 上野山賀久, 束村博子

    第26回日本生殖内分泌学会学術集会  2022.1  日本生殖内分泌学会

     More details

    Event date: 2022.1

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:石川   Country:Japan  

  3. Kiss1細胞常時可視化遺伝子改変ラットを用いた雌雄脳内Kiss1細胞分布の経時的解析 *全発表者名

    山田晃熙、長江麻佑子、眞野哲也、井上直子、上野山賀久、平林真澄、束村博

    第26回日本生殖内分泌学会学術集会  2022.1.8  日本生殖内分泌学会

     More details

    Event date: 2022.1

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:石川   Country:Japan  

  4. The Neural Pathway Mediating the Suppression of Reproductive Function during Lactation

    2022.1.7  WEB開催

     More details

    Event date: 2022.1

    Language:English   Presentation type:Poster presentation  

    Country:Japan  

  5. 遺伝子改変ラットを用いた哺乳類の卵胞発育中枢の同定

    長江麻佑子、土田仁美、井上直子、束村博子、上野山賀久

    愛知県農学系4機関による研究交流会  2021.12.17  愛知県農学系4機関

     More details

    Event date: 2021.12

    Language:Japanese   Presentation type:Poster presentation  

    Venue:WEB開催   Country:Japan  

  6. GPR75シグナリングは高脂肪食による過食・肥満・糖代謝異常を仲介する

    大塚裕記, 平林真澄, 井上直子, 束村博子, 上野山賀久

    第16回 GPCR研究会  2021.11  GPCR研究会

     More details

    Event date: 2021.11

    Language:Japanese   Presentation type:Poster presentation  

    Venue:WEB開催   Country:Japan  

  7. β-エンドルフィン-µオピオイド受容体シグナリングは低栄養による生殖機能抑制と糖新生/摂食の誘起を仲介する

    土田仁美, 井上直子, 上野山賀久, 束村博子

    第16回 GPCR研究会  2021.11  GPCR研究会

     More details

    Event date: 2021.11

    Language:Japanese   Presentation type:Poster presentation  

    Venue:WEB開催   Country:Japan  

  8. 卵胞発育を司るGnRHパルス発生機構の同定

    長江麻佑子、土田仁美、井上直子、束村博子、上野山賀久

    第45回日本比較内分泌学会  2021.11  日本比較内分泌学会

     More details

    Event date: 2021.11 - 2021.12

    Language:Japanese   Presentation type:Poster presentation  

    Venue:WEB開催   Country:Japan  

  9. 脳内β-エンドルフィン-µオピオイド受容体シグナリングがグルコース利用阻害によるLH分泌抑制/糖新生/摂食誘起を仲介する

    土田仁美, 井上直子, 上野山賀久, 束村博子

    第45回日本比較内分泌学会  2021.11  日本比較内分泌学会

     More details

    Event date: 2021.11

    Language:Japanese   Presentation type:Poster presentation  

    Venue:WEB開催   Country:Japan  

  10. 性成熟を制御する弓状核KNDyニューロン Invited

    上野山賀久、井上直子、束村博子

    第54回日本小児内分泌学会学術集会@札幌コンベンションセンター  2021.10  日本小児内分泌学会

     More details

    Event date: 2021.10

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Venue:札幌(WEB参加)   Country:Japan  

  11. 室傍核ダイノルフィンAニューロンは泌乳ラットにおけるLHパルス抑制を仲介する

    土田仁美, 井上直子, 上野山賀久, 束村博子

    第114回日本繁殖生物学会大会  2021.9  日本繁殖生物学会

     More details

    Event date: 2021.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:WEB開催   Country:Japan  

  12. GnRH パルスジェネレーター解析のための新規 Cre 発現ラットの作製

    長江麻佑子、小林俊寛、三宝誠、平林真澄、水野直彬、中内啓光、榎本悠希、井上直子、束村博子、上野山賀久

    第114回日本繁殖生物学会大会  2021.9  日本繁殖生物学会

     More details

    Event date: 2021.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:WEB開催   Country:Japan  

  13. 雌ラットキスペプチンニューロンにおけるSNAP-25遺伝子の発現解析

    山田晃熙、井上直子、上野山賀久、束村博子

    第114回日本繁殖生物学会大会  2021.9  日本繁殖生物学会

     More details

    Event date: 2021.9

    Language:Japanese   Presentation type:Poster presentation  

    Venue:WEB開催   Country:Japan  

  14. 泌乳ラットにおけるKiss1発現抑制を担うエストロゲン受容体共役コリプレッサーの探索

    滝沢麻里奈, 井上直子, 上野山賀久, 束村博子

    第114回日本繁殖生物学会大会  2021.9  日本繁殖生物学会

     More details

    Event date: 2021.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:WEB開催   Country:Japan  

  15. Search for purinergic neurons projecting to the vicinity of the kisspeptin neurons in the anteroventral periventricular nucleus by retrograde tracing

    Safiullah HAZIM, Yoshihisa UENOYAMA, Hiroko TSUKAMURA, Naoko INOUE

    2021.9 

     More details

    Event date: 2021.9

    Language:English   Presentation type:Oral presentation (general)  

    Country:Japan  

  16. β-エンドルフィン-μ-オピオイド受容体シグナリングはグルコース利用阻害による生殖機能抑制を仲介する

    土田仁美, 河合成美、井上直子, 上野山賀久, 束村博子

    第94回日本内分泌学会学術総会  2021.4  日本内分泌学会

     More details

    Event date: 2021.4

    Language:Japanese   Presentation type:Poster presentation  

    Venue:WEB開催   Country:Japan  

  17. Generation of Genetically Modified Rats for Analysis of Neural Mechanism Involved in GnRH Pulse Generation.

    Mayuko Nagae, Toshihiro Kobayashi, Makoto Sanbo, Masumi Hirabayashi, Naoko Inoue, Hiroko Tsukamura, Yoshihisa Uenoyama

    2021.1.8 

     More details

    Event date: 2021.1

  18. 室傍核ダイノルフィンニューロンがグルコース利用阻害による生殖機能抑制を仲介する

    土田 仁美,井上 直子,上野山 賀久,束村 博子

    第25回日本生殖内分泌学会  2020.12.12 

     More details

    Event date: 2020.12

    Presentation type:Oral presentation (general)  

  19. ATP-プリン受容体シグナリングによるLHサージ制御機構~ATPはキスペプチンニューロン不死化細胞のCa2+を上昇させる~

    土肥由莉、大塚裕記、上野山賀久、束村博子、井上直子

    東海畜産学会  2020.12.4 

     More details

    Event date: 2020.12

  20. 弓状核Kiss1発現を制御するエストロジェン受容体αコリプレッサーの探索

    宮崎紗衣、井上直子、束村博子、上野山賀久

    東海畜産学会  2020.12.4 

     More details

    Event date: 2020.12

  21. 低栄養による生殖機能抑制は室傍核ダイノルフィンニューロンによる弓状核キスペプチンニューロンの抑制によって仲介される

    土田 仁美,井上 直子,上野山 賀久,束村 博子

    第113回日本繁殖生物学会 

     More details

    Event date: 2020.9

    Presentation type:Poster presentation  

  22. Kiss1発現を制御するエストロジェン受容体αコリプレッサーの探索

    宮崎紗衣、井上直子、束村博子、上野山賀久

    第113回日本繁殖生物学会 

     More details

    Event date: 2020.9

  23. ATP-プリン受容体シグナリングによるAVPVキスペプチンニューロン由来不死化細胞株活性化の検討

    土肥由莉、上野山賀久、束村博子、井上直子

    第113回日本繁殖生物学会 

     More details

    Event date: 2020.9

  24. 遺伝子改変ラットを用いた卵胞発育中枢の同定

    長江麻佑子、上野山賀久、井上直子、小林憲太、平林真澄、束村博子

    第10回名古屋大学医学系研究科・生理学研究所合同シンポジウム  2020.9.19 

     More details

    Event date: 2020.9

    Language:Japanese   Presentation type:Oral presentation (general)  

  25. 排卵中枢キスペプチンニューロンを上位から制御するATP-プリン受容体シグナリング

    井上直子、土田仁美、山田晃熙、土肥由莉、上野山賀久、束村博子

    日本生殖内分泌学会  2020.1.11 

     More details

    Event date: 2020.1

    Presentation type:Oral presentation (general)  

  26. 哺乳類の排卵を制御する脳内メカニズム Invited

    井上直子、上野山賀久、束村博子

    第46回日本神経内分泌学会 シンポジウム2: Kisspeptin・生殖内分泌 

     More details

    Event date: 2019.10

    Presentation type:Symposium, workshop panel (nominated)  

  27. Brain mechanism underlying ovulation in mammals International conference

    Naoko INOUE

    International Conference on Biology and Pathology of Reproduction in Domestic Animals 

     More details

    Event date: 2015.9

    Language:English   Presentation type:Symposium, workshop panel (nominated)  

    Country:Japan  

  28. 排卵を制御する脳のメカニズム

    井上直子

    日本獣医解剖学会シンポジウム 

     More details

    Event date: 2015.9

    Language:Japanese   Presentation type:Symposium, workshop panel (nominated)  

    Country:Japan  

  29. スンクスにおける生殖制御機構

    井上直子

    スンクスを用いた脳-末梢連関研究シンポジウム 

     More details

    Event date: 2013.2

    Language:Japanese   Presentation type:Symposium, workshop panel (nominated)  

    Country:Japan  

  30. 弓状核特異的Kiss1レスキュー/ノックアウトラットを用いたGnRHパルスジェネレーターの同定

    長江麻佑子、上野山賀久、岡本沙季、土田仁美、池上花奈、後藤哲平、三宝誠、平林真澄、小林憲太、井上直子、束村博子

    第25回日本生殖内分泌学会  2020.12.12 

▼display all

Research Project for Joint Research, Competitive Funding, etc. 1

  1. スンクスにおける嗅覚系刺激によるキスペプチンニューロンを介した排卵制御機構の解明

    2013 - 2015

    内藤記念女性研究者研究助成金 

      More details

    Grant type:Competitive

KAKENHI (Grants-in-Aid for Scientific Research) 9

  1. InvivoRNAサイレンシング法を活用した哺乳類卵胞発育の人為的制御技術の創出

    2005.4 - 2007.3

    科学研究費補助金  若手研究(B),課題番号:17780216

    井上 直子

      More details

    Authorship:Principal investigator 

  2. 東南アジアにおけるマメジカ類の生理生態学的研究と増殖保存に関する研究

    2006.4 - 2008.3

    科学研究費補助金  基盤研究(B)

    福田勝洋

      More details

    Authorship:Coinvestigator(s) 

  3. 食虫目スンクスにおける妊娠維持機構の解析と着床因子の探索

    2007.4 - 2009.3

    科学研究費補助金  若手研究(B),課題番号:19780215

      More details

    Authorship:Principal investigator 

  4. 家畜の排卵・卵胞発育制御法の開発に資するエストロゲンフィードバック機構の解明

    Grant number:21H05031  2021.7 - 2026.3

    科学研究費助成事業  基盤研究(S)

    束村 博子, 大蔵 聡, 平林 真澄, 羽田 真悟, 真方 文絵, 上野山 賀久, 大石 真也, 井上 直子, 中村 翔

      More details

    Authorship:Coinvestigator(s) 

    家畜やヒトを含む哺乳類メスにおける卵胞発育と排卵を制御するエストロゲンのフィードバック機構に着目し、卵胞発育を支配する負のフィードバック、および排卵を支配する正のフィードバックによる性腺刺激ホルモン分泌制御の分子メカニズムの解明とその応用の検証を目的とする。生殖機能中枢であるキスペプチンニューロンでのキスペプチン遺伝子発現とその分泌に対するエストロゲンの促進・抑制作用を、2つの神経核に局在するキスペプチンニューロンにおける神経核特異的な転写因子、受容体、分泌制御因子等の作用による制御の解明により明らかとし、家畜における実証研究により、排卵・卵胞発育障害などに対する治療法の開発に資する。

  5. 繁殖中枢を不可逆に抑制するエストロゲンによる発達脳プログラミング機構の解明

    Grant number:21K19186  2021.7 - 2023.3

    科学研究費助成事業  挑戦的研究(萌芽)

    束村 博子, 上野山 賀久, 井上 直子

      More details

    Authorship:Coinvestigator(s) 

    哺乳類において発達脳への性ステロイドホルモン感作が、生涯にわたって生殖機能を抑制する分子メカニズムの解明を目的とする。具体的には、発達期の脳へのエストロゲン感作により、キスペプチンニューロンにおけるKiss1発現が特異的かつ不可逆に消失するメカニズムを解明する。そのために(1)キスペプチンニューロンを常時可視化できるトランスジェニック(Tg)ラットの作出、(2)発達脳でエストロゲンが活性化する候補遺伝子群のリスト化、(3)In vitro細胞培養系および組織学的解析による候補遺伝子群の絞り込み、(4)キスペプチンニューロン特異的な候補遺伝子KOラット作製による候補因子の役割解明を実施する。

  6. 温暖化による家畜繁殖能低下の克服に資する脳熱センサーの繁殖中枢調節システムの解明

    Grant number:20H03127  2020.4 - 2024.3

    科学研究費補助金  基盤研究(B)

    上野山 賀久、平林真澄、森田康広、中村翔

      More details

    Authorship:Coinvestigator(s)  Grant type:Competitive

  7. ATP-プリン受容体シグナリングによる哺乳類の排卵中枢制御メカニズムの解明

    2019.4 - 2023.3

    科学研究費補助金  基盤研究(B)

    井上 直子

      More details

    Authorship:Principal investigator  Grant type:Competitive

  8. 食虫目スンクスを用いた新規な遺伝子改変モデル動物の開発

    2011.4 - 2014.3

    科学研究費補助金 

      More details

    Authorship:Coinvestigator(s) 

  9. 交尾排卵動物モデル・スンクスを用いた排卵の神経メカニズムの解明

    2011.4 - 2014.3

    科学研究費補助金  若手研究(B)

      More details

    Authorship:Principal investigator 

▼display all

 

Teaching Experience (On-campus) 30

  1. Science of Animals and Plants

    2020

  2. 修士論文研究4

    2020

  3. 修士論文研究3

    2020

  4. 修士論文研究2

    2020

  5. 生命農学演習4

    2020

  6. 理系基礎(生物学実験)

    2020

  7. 資源生物科学実験実習

    2020

  8. Basics of Bioagricultural Sciences

    2020

  9. 動物生産科学1

    2016

  10. 動植物の科学

    2016

  11. Basics of Bioagricultural Sciences

    2016

  12. 動物生産科学3

    2016

  13. 動物生産科学1

    2015

  14. 理系基礎(生物学実験)

    2015

  15. 動植物の科学

    2015

  16. 資源生物科学実験実習

    2014

  17. 理系基礎(生物学実験)

    2014

  18. 資源生物科学セミナー

    2014

  19. 資源生物科学実験実習

    2013

  20. 理系基礎(生物学実験)

    2013

  21. 資源生物科学セミナー

    2013

  22. 理系基礎(生物学実験)

    2011

  23. 資源生物科学実験実習

    2011

  24. 資源生物科学セミナー

    2011

  25. 資源生物科学セミナー

    2010

  26. 理系基礎(生物学実験)

    2010

  27. 資源生物科学実験実習

    2010

  28. 資源生物科学セミナー

    2009

  29. 理系基礎(生物学実験)

    2009

  30. 資源生物科学実験実習

    2009

▼display all

 

Social Contribution 6

  1. 名古屋大学教育学部附属中・高等学校のスーパーサイエンスハイスクール (SSH)事業「学びの杜・学術コース」「生命科学探究講座」

    Role(s):Lecturer

    2019.8

  2. 女子中高生理系進学推進セミナー

    Role(s):Lecturer

    2018.8

  3. 名古屋大学教育学部附属中・高等学校のスーパーサイエンスハイスクール (SSH)事業「学びの杜・学術コース」「生命科学探究講座」

    Role(s):Lecturer

    2018.8

  4. 名古屋大学教育学部附属中・高等学校のスーパーサイエンスハイスクール (SSH)事業「学びの杜・学術コース」「生命科学探究講座」

    Role(s):Lecturer

    2017.8

  5. 滋賀県立彦根東高校SSH受け入れ

    2013.12

  6. 滋賀県立彦根東高校SSH受け入れ

    2011.8

▼display all

Media Coverage 2

  1. Recovery of ovarian function in infertile mammals lacking gonadotropin release Internet

    EulekAlert!  https://www.eurekalert.org/pub_releases/2021-01/nu-roo012121.php  2021.1

     More details

    Author:Other 

  2. ネズミの生殖機能回復研究で成功

    NHK  2020.2

     More details

    Author:Other