Updated on 2026/03/09
Ph. D. （ 1997.3 Nagoya University ）
Others / Other / Applied Biochemistry
Others / Other / Applied Molecular Cell Biology
新奇骨誘導タンパク質NELL1の機能解析
基底膜蛋白質ラミニンの大量生産系の構築
名古屋大学大学院生命農学研究科講師
2016.4
Country：Japan
名古屋大学大学院生命農学研究科助教
2007.4 - 2015.3
Country：Japan
名古屋大学大学院生命農学研究科助手
2004.4 - 2007.3
Country：Japan
Researcher, Sekiguchi Biomatrix Signaling Project, ERATO, Japan Science and Technology Agency
2001.3 - 2004.3
Country：Japan
Visiting Fellow, National Cancer Institute
1998.10 - 2001.2
Country：Japan
Researcher, Nagoya University BioSciences Center
1997.4 - 1998.9
Country：Japan
Nagoya University Graduate School, Division of Agriculture Graduate program for Biochemical Regulation
- 1997
Country： Japan
Nagoya University Faculty of Agriculture Department of Food Science
- 1991
Country： Japan
日本農芸化学会
The Molecular Biology Society of JAPAN
Glu592 of the axon guidance receptor ROBO3 mediates a pH-dependent interaction with NELL2 ligand Open Access
Mizutani, K; Toyoda, M; Ojima-Kato, T; Maturana, AD; Niimi, T
FEBS LETTERS Vol. 599 ( 4 ) page： 571 - 580 2025.2
Conformational change of the hairpin-like structured Robo2 ectodomain allows NELL1/2 binding. Reviewed
1. Miyaguchi, M., Nakanishi, Y., Maturana, A.D., Mizutani, K., and Niimi, T.
Journal of Molecular Biology Vol. 434 page： 167777 2022.10
Roles of Slit Ligands and Their Roundabout (Robo) Family of Receptors in Bone Remodeling. Invited Reviewed
Advances in Experimental Medicine and Biology Vol. 21 page： 143 - 154 2021
Robo2 contains a cryptic binding site for neural EGFL-like (NELL) protein 1/2 Open Access
Yamamoto, N; Kashiwagi, M; Ishihara, M; Kojima, T; Maturana, AD; Kuroda, S; Niimi, T
JOURNAL OF BIOLOGICAL CHEMISTRY Vol. 294 ( 12 ) page： 4693 - 4703 2019.3
pH依存的な結合性を示す軸索ガイダンス分子群の探索解析
新美友章
メディカル・サイエンス・ダイジェスト Vol. 51 ( 3 ) page： 48 - 49 2025.2
骨形成を促進する新奇因子NELL1の骨誘導分子基盤の解明
新美友章
メディカル・サイエンス・ダイジェスト Vol. 47 ( 2 ) page： 106 - 107 2021.2
Morinaga, A; Ito, J; Niimi, T; Maturana, AD
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Vol. 20 ( 22 ) 2019.11
Scaffold protein Enigma Homolog activates CREB whereas a short splice variant prevents CREB activation in cardiomyocytes.
Ito, J., Iijima, M., Yoshimoto, N., Niimi, T., Kuroda, S., Maturana, A. D.
Cell. Signal. Vol. 27 page： 2425-2433 2015.12
Mapping the heparin-binding site of the osteoinductive protein NELL1 by site-directed mutagenesis. Reviewed
Takahashi, K., Imai, A., Iijima, M., Yoshimoto, N., Maturana, A. D., Kuroda, S., and Niimi, T.
FEBS Letters Vol. 589 page： 4026-4032 2015.12
Intracellular trafficking of bio-nanocapsule-liposome complex: Identification of fusogenic activity in the pre-S1 region of hepatitis B virus surface antigen L protein. Reviewed
Somiya, M., Sasaki, Y., Matsuzaki, T., Iijima, M., Yoshimoto, N., Niimi, T., Maturana, A. D., and Kuroda, S.
J. Controll. Release Vol. 212 page： 10-18 2015.8
One-step scalable preparation method for non-cationic liposomes with high siRNA content. Reviewed
Somiya, M., Yamaguchi, K., Liu, Q., Niimi, T., Maturana, A. D., Iijima, M., Yoshimoto, N., and Kuroda, S.
Int. J. Pharm. Vol. 490 page： 316-323 2015.7
Virosomes of hepatitis B virus envelope L proteins containing doxorubicin: synergistic enhancement of human liver-specific anti-tumor growth activity by radiotherapy. Reviewed Open Access
Liu, Q., Jung, J., Somiya, M., Iijima, M., Yoshimoto, N., Niimi, T., Maturana, A. D., Shin, S. H., Jeong, S.-Y., Choi, E. K., and Kuroda, S.
Int. J. Nanomedicine Vol. 10 page： 4159-4172 2015.6
Oligomerization-induced conformational change in the C-terminal region of Nel-like molecule 1 (NELL1) protein is necessary for the efficient mediation of murine MC3T3-E1 cell adhesion and spreading. Reviewed
Nakamura, Y., Hasebe, A., Takahashi, K., Iijima, M., Yoshimoto, N., Maturana, A. D., Ting, K., Kuroda, S., and Niimi, T.
J. Biol. Chem. Vol. 289 page： 9781-9794 2014.4
High-throughput de novo screening of receptor agonists with an automated single-cell analysis and isolation system. Open Access
Yoshimoto, N., Tatematsu, K., Iijima, M., Niimi, T., Maturana, A. D., Fujii, I., Kondo, A., Tanizawa, K., and Kuroda, S.
Vol. 4 page： 4242 2014
Cell surface-fluorescence immunosorbent assay for real-time detection of hybridomas with efficient antibody secretion at the single-cell level. Anal. Chem. Reviewed
Kida, A., Iijima, M., Niimi, T., Maturana, A. D., Yoshimoto, N., and Kuroda, S.
Anal. Chem. Vol. 85 page： 1753-1759 2013
Exploring dynamics of molybdate in living animal cells by a genetically encoded FRET nanosensor. Reviewed Open Access
Nakanishi, Y., Iida, S., Ueoka-Nakanishi, H., Niimi, T., Tomioka, R., and Maeshima, M.
PLoS One. Vol. 8 page： e58175 2013
An automated system for high-throughput single cell-based breeding. Reviewed Open Access
Yoshimoto, N., Kida, A., Jie, X., Kurokawa, M., Iijima, M., Niimi, T., Maturana, A. D., Nikaido, I., Ueda, H. R., Tatematsu, K., Tanizawa, K., Kondo, A., Fujii, I., and Kuroda, S.
Sci. Rep. Vol. 3 page： 1191 2013
Nanocapsule-based probe for evaluating the orientation of antibodies immobilized on a solid phase. Reviewed
Iijima, M., Yoshimoto, N., Niimi, T., Maturana, A. D., and Kuroda, S.
Analyst. Vol. 138 page： 3470-3477 2013
Bio-nanocapsules for signal enhancement of alkaline phosphatase-linked immunosorbent assays. Reviewed
Iijima, M., Yamamoto, M., Yoshimoto, N., Niimi, T., and Kuroda, S.
Biosci. Biotechnol. Biochem. Vol. 77 page： 843-846 2013
Nano-visualization of oriented-immobilized IgGs on immunosensors by high-speed atomic force microscopy Open Access
Iijima, M., Somiya, M., Yoshimoto, N., Niimi, T., and Kuroda, S. (2012). Nano-visualization of oriented-immobilized IgGs on immunosensors by high-speed atomic force microscopy.
Sci. Rep. Vol. 2 page： 790 2012
NELL-1 promotes cell adhesion and differentiation via Integrin β1 Reviewed
Shen, J., James, A. W., Chung, J., Lee, K., Zhang, J. B., Ho, S., Lee, K. S., Kim, T. M., Niimi, T., Kuroda, S., Ting, K., and Soo
J. Cell. Biochem. Vol. 113 page： 3620-3628 2012
The C-terminal region of NELL1 mediates osteoblastic cell adhesion through integrin α3β1 Reviewed Open Access
Hasebe, A., Nakamura, Y., Tashima, H., Takahashi, K., Iijima, M., Yoshimoto, N., Ting, K., Kuroda, S., and Niimi, T.
FEBS Lett. Vol. 586 page： 2500-2506 2012
Engineered bio-nanocapsules, hepatitis B virus surface antigen L protein particles, for in vivo active targeting to splenic dendritic cells. Reviewed
Matsuo, H., Yoshimoto, N., Iijima, M., Niimi, T., Jung, J., Jeon, S.-Y., Choi, E. K., Sewaki, T., Arakawa, T., and Kuroda, S.
Int. J. Nanomedicine. Vol. 7 page： 3341-3350 2012
Targeting of polyplex to human hepatic cells by bio-nanocapsules, hepatitis B virus surface antigen L protein particles Reviewed
Somiya, M., Yoshimoto, N., Iijima, M., Niimi, T., Dewa, T., Jung, J., and Kuroda, S.
Bioorg. Med. Chem. Vol. 20 page： 3873-3879 2012
Hepatitis B virus envelope L protein-derived bio-nanocapsules: Mechanisms of cellular attachment and entry into human hepatic cells Reviewed
Yamada, M., Oeda, A., Jung, J. Iijima, M., Yoshimoto, N., Niimi, T., Jeong, S.-Y., Choi, E. K., Tanizawa, K., and Kuroda, S.
J. Control. Release Vol. 160 page： 322-329 2012
Efficient production and characterization of recombinant human NELL1 protein in human embryonic kidney 293-F cells. Reviewed
Hasebe, A., Tashima, H., Ide, T., Iijima, M., Yoshimoto, N., Ting, K., Kuroda, S., and Niimi, T.
Mol. Biotechnol. Vol. 51 page： 58-66 2012
Nanocapsules incorporating IgG Fc-binding domain derived from Staphylococcus aureus protein A for displaying IgGs on immunosensor chips Reviewed
Iijima, M., Kadoya, H., Hatahira, S., Hiramatsu, S., Jung, G., Martin, A., Quinn, J., Jung, J., Jeong, S. Y., Choi, E. K., Arakawa, T., Hinako, F., Kusunoki, M., Yoshimoto, N., Niimi, T., Tanizawa, K., and Kuroda, S.
Biomaterials Vol. 32 page： 1455-1464 2011
Fluorophore-labeled nanocapsules displaying IgG Fc-binding domains for the simultaneous detection of multiple antigens. Reviewed
Iijima, M., Matsuzaki, T., Yoshimoto, N., Niimi, T., Tanizawa, K., and Kuroda, S.
Biomaterials Vol. 32 page： 9011-9020 2011
Efficient and rapid purification of drug- and gene-carrying bio-nanocapsules, hepatitis B virus surface antigen L particles, from Saccharomyces cerevisiae Reviewed
Jung, J., Iijima, M., Yoshimoto, N., Sasaki, M., Niimi, T., Tatematsu, K., Jeong, S.-Y., Choi, E. K., Tanizawa, K., and Kuroda, S.
Protein Expr. Purif. Vol. 78 page： 149-155 2011
Hepatic stellate cells stimulate HCC cell migration via laminin-5 production Reviewed Open Access
Santamato, A., Fransvea, E., Dituri, F., Caligiuri, A., Quaranta, M., Niimi, T., Pinzani, M., Antonaci, S., and Giannelli, G.
Clin. Sci. Vol. 121 page： 159-168 2011
*The production of recombinant human laminin-332 in a Leishmania tarentolae expression system Reviewed
Phan, H.P., Sugino, M. and Niimi, T.
Protein Expr. Purif. Vol. 68 page： 79-84 2009
Novel Culture System of Mesenchymal Stromal Cells from Human Subcutaneous Adipose Tissue Reviewed
Iwashima, S., Ozaki, T., Maruyama, S., Saka, Y., Kobori, M., Omae, K., Yamaguchi, H., Niimi, T., Toriyama, K., Kamei, Y., Torii, S., Murohara, T., Yuzawa, Y., Kitagawa, Y. and Matsuo, S.
Stem Cells Dev. Vol. 18 page： 533-543 2009
*Transcriptional activation of the human claudin-18 gene promoter through two AP-1 motifs in PMA-stimulated MKN45 gastric cancer cells Reviewed
Yano, K., Imaeda, T., and Niimi, T.
Am. J. Physiol. Gastrointest. Liver Physiol. Vol. 294 page： G336-G343 2008
Expression of secretoglobin3A2 (SCGB3A2) in primary pulmonary carcinomas Reviewed
Tachihara-Yoshikawa, M., Ishida, T., Watanabe, K., Sugawara, A., Kanazawa, K., Kanno, R., Suzuki, T., Niimi, T., Kimura, S. and Munakata, M.
Fukushima J. Med. Sci. Vol. 54 page： 61-72 2008
*Expression and chain assembly of human laminin-332 in an insect cell-free translation system Reviewed Open Access
Phan, H.-P., Ezure, T., Ito, M., Kadowaki, T., Kitagawa, Y., and Niimi, T.
Biosci. Biotechnol. Biochem. Vol. 72 page： 1847-1852 2008
Cryptic fragment alpha4 LG4-5 derived from laminin alpha4 chain inhibits de novo adipogenesis by modulating the effect of fibroblast growth factor-2 Reviewed
Yamashita, H., Goto, C., Tajima, R., Koparal, A. T., Kobori, M., Ohki, K., Shitara, K., Narita, R., Toriyama, K., Torii, S., Niimi, T., and Kitagawa, Y.
Develop. Growth Differ. Vol. 50 page： 97-107 2008
Identification and expression of alternative splice variants of the mouse Ppp1r3b gene in lung epithelial cells Reviewed
Niimi, T., Kurotani, R., Kimura, S., and Kitagawa, Y.
Biochem. Biophys. Res. Commun. Vol. 349 page： 588-596 2006
The Sp family of transcription factors regulates the human laminin alpha 1 gene in JAR choriocarcinoma cells Reviewed
Niimi, T., Hayashi, Y., Sekiguchi, K., and Kitagawa, Y.
Biochim. Biophys. Acta. Vol. 1759 page： 573-579 2006
Induction of uteroglobin-related protein 2 (Ugrp2) gene expression by the Th2 cytokines IL-4 and IL-13 Reviewed Open Access
Yamada, A., Sheikh, F., Niimi, T., DeMayo, F. J., Keegan, A. D., Donnelly, R. P., Kimura, S.
J. Immunol. Vol. 175 page： 5708-5715 2005
SOX7 and SOX17 regulate the parieetal endoderm-specific enhancer activity of mouse laminin a1 gene Reviewed
Niimi, T., Hayashi, Y., Futaki, S., and Sekiguchi, K.
J. Biol. Chem Vol. 279 ( 36 ) page： 38055-38061 2004
Identification of an upstream enhancer in the mouse laminin alpha1 gene defining its high level of expression in parietal endoderm cells Reviewed
Niimi, T. Hayashi, Y., and Sekiguchi, K.
J. Biol. Chem. Vol. 14 page： 9332-9338 2003
A polymorphism in the human UGRP1 gene promoter that regulates transcription is associated with an increased risk of asthma Reviewed Open Access
Niimi, T., Munakata, M., Keck-Waggoner, C. L., Popescu, N. C., Levitt, R. C., Hisada, M., and Kimura, S.
Am. J. Hum. Genet. Vol. 70 page： 718-725 2002
Cloning, expression, and chromosomal localization of the mouse gene (Scgb3a1, alias Ugrp2) that encodes a member of the novel uteroglobin-related protein gene family Reviewed Open Access
Niimi, T., Copeland, N. G., Gilbert, D. J., Jenkins, N. A., Srisodsai, A., Zimonjic, D. B., Keck-Waggoner, C. L., Popescu, N. C., and Kimura, S.
Cytogenet. Genome Res. Vol. 97 page： 120-127 2002
claudin-18, a novel downstream target gene for the T/EBP/NKX2.1 homeodomain transcription factor, encodes lung-and stomach-specific isoforms through alternative splicing Reviewed
Niimi, T., Nagashima, K., Ward, J. M., Minoo, P., Zimonjic, D. B., Popescu, N. C., and Kimura, S.
Mol. Cell. Biol. Vol. 21 page： 7380-7390 2001
UGRP1, a uteroglobin/clara cell secretory protein-related protein, is a novel lung-enriched downstream target gene for the T/EBP?NKX2.1 homeodomain transcription factor Reviewed
Niimi, T., Keck-Waggoner, C. L., Popescu, N. C., Zhou, Y., Levitt, R. C., and Kimura, S.
Molecular Endocrinology Vol. 15 page： 2021-2036 2001
Differential expression of mRNAs encoding laminin chain variants during in vitro development of mouse blastocysts Reviewed
Azimi, M., Niimi, T., Yoshida, N., and Kitagawa, Y.
Cytotechnology Vol. 31 page： 183-191 1999
A drosophila gene encoding multiple splice variants of Kazal-type serine protease inhibitor-like proteins with potential destinations of mitochondria, cytosol and the secretory pathway Reviewed
Niimi, T., Yookoyama, H., Goto, A., Beck, K., and Kitagawa, Y.
Eur. J. Biochem. Vol. 266 page： 282-292 1999
Brachyury (T) expression in embryonal carcinoma P19 cells resembles its expression in primitive streak and tail-bud but not that in notochord Reviewed
Yamaguchi, H., Niimi, T., Kitagawa, Y., and Miki, K.
Develop. Growth Differ. Vol. 41 page： 253-264 1999
Distinct roles of mouse laminin beta1 long arm domains for alpha1beta1gamma1 trimer formation Reviewed
Niimi, T. and Kitagawa, Y.
FEBS Letters Vol. 400 page： 71-74 1997
多様なラミニン分子の形成機構
新美友章、熊谷知乃、北川泰雄
生体の科学 Vol. 48 page： 260-267 1997
Differentiation-dependent expression of laminin-8 (alpha4beta1gamma1) mRNAs in mouse 3T3-L1 adipocytes Reviewed
Niimi, T., Kumagai, C., Okano, M., and Kitagawa, Y.
Matrix Biology Vol. 16 page： 223-230 1997
Expression of the long arm sequence of mouse laminin alpha1, beta1, or gamma1 chain in COS1 cells and assembly of monkey-mouse hybrid laminin Reviewed
Niimi, T., Miki, K., and Kitagawa, Y.
J. Biochem Vol. 121 page： 854-861 1997
alpha-へリックス鎖間会合の特異性と互換性-ラミニン異型体の形成機構を中心に- Open Access
北川泰雄、新美友章、熊谷知乃、岡野正樹
生物物理 Vol. 35 page： 63-69 1995
Regulated expression of an osteonectin variant in bovine aortic endothelial cells Reviewed
Jeon, H., Niimi, T., Taniguchi, Y., Miki, K., and Kitagawa, Y.
Biosci. Biotech. Biochem. Vol. 58 ( 10 ) page： 1889-1891 1994
The protective role of polyphenols in cytotoxicity of hydrogen peroxide Reviewed
Nakayama, T., Niimi, T., Osawa, T., and Kawakishi, S.
Mutation Research Vol. 281 page： 77-80 1992
Leishmania tarentolae for the production of multi-subunit complexes, Advances in Experimental Medicine and Biology.
Niimi, T.（ Role： Contributor）
Springer 2016
Recombinant protein production in the eukaryotic protozoan parasite Leishmania tarentolae: A review. In: Recombinant Gene Expression: Reviews and Protocols, Third Edition, Methods in Molecular Biology.
Niimi, T.（ Role： Sole author）
Humana Press 2012
Expression of multi-subunit proteins in Leishmania tarentolae. In: Recombinant Gene Expression: Reviews and Protocols, Third Edition, Methods in Molecular Biology.
Sugino, M. and Niimi, T. （ Role： Joint author）
Humana Press 2012
細胞外マトリックス-基礎と臨床 「基底膜に存在する糖タンパク質」
熊谷知乃、新美友章、北川泰雄（ Role： Joint author）
愛智出版 2000
Extracellular Matrix-Cellular Interaction: Molecules to Diseases"Role of interchain disulfide-bonding in dimer-trimer switch during laminin heterotrimer assembry."
kitagawa, Y., Niimi, T., and Kumagai, C.（ Role： Joint author）
Japan Sci. Soc. Press, Tokyo/ S. Karger, Basel 1998
Animal Cell Technology; Basic & Applied Aspects"Laminin chains expressed in human keratinocytes, mouse 3T3-L1 adipocytes and mouse embryonal carcinoma F9."
Niimi, T., Kumagai, C., Azimi, M., and Kitagawa, Y.（ Role： Joint author）
Kluwe Academic Publisher, Dordrecht 1997
骨分化誘導タンパク質NELL1の新奇受容体候補Robo2の構造特性解析
宮口昌樹、中西洋一、新美友章
第44回日本分子生物学会年会 2021.12
タイトジャンクション構成蛋白質・クローディン-18a2を指標にした胃癌の新しい分子標的療法の開発
2006.4 - 2007.3
上原記念生命科学財団
Grant type：Competitive
膜性腎症における抗NELL1抗体のエピトープ解析と腎炎惹起性NELL1ドメインの探索
Grant number：25K11511 2025.4 - 2028.3
科学研究費助成事業 基盤研究(C)
林 憲史, 新美 友章
Authorship：Coinvestigator(s)
Neural epidermal growth factor-like 1 protein (NELL1)は2021年に膜性腎症の新規抗原として報告されたが、その病態形成機序は不明である。本研究は、組み換えNELL1タンパクを用いて患者血清中の抗NELL1抗体のエピトープを解析すること、さらにエピトープタンパクをマウスに投与しNELL1陽性膜性腎症モデルマウスを作製し、NELL1陽性膜性腎症の病態形成機序解明の一旦を担うことを目標とする。
脳内pH低下に伴うNELL－ROBOシグナルの変容と神経細胞機能の統合的理解
Grant number：24K08712 2024.4 - 2027.3
科学研究費助成事業 基盤研究(C)
新美 友章, 水谷 公彦
Authorship：Principal investigator Grant type：Competitive
Grant amount：\4680000 （ Direct Cost: \3600000 、 Indirect Cost：\1080000 ）
神経軸索誘導分子であるNELL－ROBO複合体のpH依存的な結合について、脳内における生理学的意義を解明するために、pH変化に伴うNELL－ROBO複合体の構造特性解析、および NELL－ROBOシグナルの変容が神経細胞機能に及ぼす影響の解析を行い、これらが脳内pHの低下と精神・神経疾患を関連付ける傍証となり得るのか検証することを試みる。
脳内pHの変化は、脳内の遺伝子発現をはじめとする様々な生物学的プロセスに関わっていると考えられ、近年では統合失調症などの精神疾患と関連していることを示唆する研究結果が増えてきているが、脳の酸性度がこれらの疾患に及ぼす影響の詳細はわかっていない。我々は、神経軸索誘導に関わるNELLリガンドとその受容体であるROBOファミリーの結合がpHの低下に伴って増強されることを発見し、このようなpH依存的なリガンド－受容体の相互作用が、脳の酸性化が見られる疾患に少なからず関係していると考え、本研究では、神経軸索誘導分子群のリガンド－受容体複合体のpH依存的な結合特性を解析すること、およびpH変化に伴うシグナルの変容が神経細胞機能に及ぼす影響を解析することを目的とした。
昨年度は、おもに神経軸索誘導に関わる分子群を対象に、酸性条件で結合性が変化するリガンド－受容体ペアの探索を行い、SLIT－ROBO系、Semaphorin－Plexin系、Ephrin－Eph系、およびNetrin－DCC/Unc5系のおもな遺伝子の発現ベクターへのクローニング、組換えタンパク質の発現、結合アッセイによるスクリーニングを終え、酸性で結合性が変化する複数のリガンド－受容体ペアを同定した。さらに、これらのリガンド－受容体の複合体形成における結合部位の解析、および酸性条件下で結合性が変化するメカニズムについての解析を進めた。これらの研究成果は、pH依存的なリガンド－受容体の相互作用がNELL－ROBO系だけに留まらず、他の神経軸索誘導分子群に広く存在していることを示しており、pH低下によるネガティブな影響のみならず、pHによるシグナル伝達制御機構が存在することをも示唆している。
当初の目論見通り、酸性条件下で結合性が変化するリガンド－受容体複合体が、神経軸索誘導分子群において、複数発見された。これらの中には、酸性で結合性が低下するものと、酸性で結合性が上昇するものが含まれている。現在、これらのリガンド－受容体の複合体形成における結合部位の解析、および酸性条件下で結合性が変化するメカニズムについての解析を進めており、研究の進捗状況は概ね順調である。
神経軸索誘導分子群のリガンド－受容体複合体のpH依存的な結合特性の解析を進める。NELL－ROBO系については、ROBO1、ROBO2、ROBO3のpH依存的な結合特性の比較解析を行う。新たに同定されたリガンド－受容体複合体については、複合体形成における結合部位の解析、および酸性で結合性が変化するメカニズムについて解析を進めているが、当然ながらタンパク質毎に固有のメカニズムが存在していると思われ、結合性が強いものから弱いものまであるため、個別に解析を進める方向とし、興味深い複合体に関しては、結晶構造解析を行うことを検討する。
pH変化に伴う神経軸索誘導分子のシグナル変容が神経細胞機能に及ぼす影響の解析については、まずはpH変化に寛容な培養細胞系を用いて実験系の確立を試みる予定である。
Functional analysis of a novel NELL-Robo signaling system in osteogenesis
Grant number：20H02948 2020.4 - 2024.3
Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)
Niimi Tomoaki
Authorship：Principal investigator Grant type：Competitive
Grant amount：\17550000 （ Direct Cost: \13500000 、 Indirect Cost：\4050000 ）
The NELL1 gene was originally identified in craniosynostosis patients as being specifically upregulated within prematurely fusing sutures. Because of its potent osteoinductive activity, NELL1 protein may be useful for bone regeneration therapy. However, there is little knowledge regarding NELL1-mediated signaling pathways. In this study, we focused on the property that NELL1 and its novel receptor, ROBO2, bind only in acidic conditions to elucidate the signaling mechanism of the NELL－ROBO complex. The results revealed that the NELL1/2 and ROBO1/2/3 families bind in a pH-dependent manner, suggesting the existence of a pH-dependent signal transduction regulatory mechanism.
Elucidation of the molecular basis of the bone-inducing factor NELL1
Grant number：17K01946 2017.4 - 2021.3
Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)
Niimi Tomoaki
Authorship：Principal investigator
Grant amount：\4680000 （ Direct Cost: \3600000 、 Indirect Cost：\1080000 ）
The NELL1 gene was originally identified in craniosynostosis patients as being specifically upregulated within prematurely fusing sutures. Because of its potent osteoinductive activity, NELL1 protein may be useful for bone regeneration therapy. However, there is little knowledge regarding NELL1 receptors and NELL1-mediated signaling pathways. IAs a result of exploring the NELL1 receptor and elucidating the signal transduction mechanism, we identified Roundabout 2 (Robo2), which is mainly expressed in mesenchymal cells, as a novel receptor for NELL1. Furthermore, we elucidated the mode of ligand-receptor binding only under acidic conditions and suggested that it may act as a new bone formation signal.
NELL1タンパク質を利用した新規骨形成因子の創成
2014.4 - 2017.3
科学研究費補助金 基盤研究(C)
Authorship：Principal investigator
酵母で量産したヒト型ラミニンによる再生医療の新展開
2007.3 - 2010.3
科学研究費補助金 基盤研究(S)
新美友章
Authorship：Principal investigator
酵母で量産したヒト型ラミニンによる再生医療の新展開
2006.4 - 2007.3
科学研究費補助金 基盤研究(S)
北川泰雄
Authorship：Coinvestigator(s)
肺および甲状腺特異的転写因子Nkx2.1とその下流遺伝子による器官形成と機能調節
2005.4 - 2007.3
科学研究費補助金 若手研究（Ｂ），課題番号：17780250
新美 友章
Authorship：Principal investigator
Advanced Molecular Cell Biology 2
2025
Biochemistry 2
2025
Molecular Cell Biology 4
2025
Biostatistics Exercise
2025
Laboratory in Chemistry
2025
生物情報処理演習（生命）
2024
化学実験
2024
分子細胞生物学4
2024
分子細胞生物学特論II
2021
分子細胞生物学特論II
2020
生物情報処理演習
2019
分子細胞生物学特論II
2019
基礎セミナーA
2019
分子細胞生物学特論II
2018
生物情報処理演習
2018
日本農芸化学会中部支部庶務幹事
Role(s)：Planning, management, etc.
2020.4 - 2022.3
