Faculty Profiles - SAZUKA, Takashi

Papers - SAZUKA, Takashi

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An analysis of sugary endosperm in sorghum: Characterization of mutant phenotypes depending on alleles of the corresponding starch debranching enzyme Reviewed

Shumpei Hashimoto, Satoshi Okada, Satoko Araki-Nakamura, Kozue Ohmae-Shinohara, Kotaro Miura, Hideo Kawaguchi, Chiaki Ogino, Shigemitsu Kasuga, Takashi Sazuka

Frontiers in Plant Science Vol. 14 page： 1114935 2023.2

Authorship：Last author,　Corresponding author Language：English Publishing type：Research paper (scientific journal) Publisher：Frontiers Media SA

Sorghum is the fifth most important cereal crop. Here we performed molecular genetic analyses of the ‘SUGARY FETERITA’ (SUF) variety, which shows typical sugary endosperm traits (e.g., wrinkled seeds, accumulation of soluble sugars, and distorted starch). Positional mapping indicated that the corresponding gene was located on the long arm of chromosome 7. Within the candidate region of 3.4 Mb, a sorghum ortholog for maize Su1 (SbSu) encoding a starch debranching enzyme ISA1 was found. Sequencing analysis of SbSu in SUF uncovered nonsynonymous single nucleotide polymorphisms (SNPs) in the coding region, containing substitutions of highly conserved amino acids. Complementation of the rice sugary-1 (osisa1) mutant line with the SbSu gene recovered the sugary endosperm phenotype. Additionally, analyzing mutants obtained from an EMS-induced mutant panel revealed novel alleles with phenotypes showing less severe wrinkles and higher Brix scores. These results suggested that SbSu was the corresponding gene for the sugary endosperm. Expression profiles of starch synthesis genes during the grain-filling stage demonstrated that a loss-of-function of SbSu affects the expression of most starch synthesis genes and revealed the fine-tuned gene regulation in the starch synthetic pathway in sorghum. Haplotype analysis using 187 diverse accessions from a sorghum panel revealed the haplotype of SUF showing severe phenotype had not been used among the landraces and modern varieties. Thus, weak alleles (showing sweet and less severe wrinkles), such as in the abovementioned EMS-induced mutants, are more valuable for grain sorghum breeding. Our study suggests that more moderate alleles (e.g. produced by genome editing) should be beneficial for improving grain sorghum.

DOI： 10.3389/fpls.2023.1114935

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ソルガムの雑種強勢メカニズムとバイオマスデザイニング Invited Reviewed

橋本舜平、佐塚隆志

アグリバイオ Vol. 7 ( 6 ) page： 8 - 12 2023.6

The dominance model for heterosis explains culm length genetics in a hybrid sorghum variety Reviewed International journal

Hashimoto Shumpei, Wake Tatsuro, Nakamura Haruki, Minamiyama Masaki, Araki-Nakamura Satoko, Ohmae-Shinohara Kozue, Koketsu Eriko, Okamura Shinnosuke, Miura Kotaro, Kawaguchi Hideo, Kasuga Shigemitsu, Sazuka Takashi

SCIENTIFIC REPORTS Vol. 11 ( 1 ) 2021.2

Sorghum DW1 positively regulates brassinosteroid signaling by inhibiting the nuclear localization of BRASSINOSTEROID INSENSITIVE 2 Reviewed

Hirano K, Kawamura M, Araki-Nakamura S, Fujimoto H, Ohmae-Shinohara K, Yamaguchi M, Fujii A, Sasaki H, Kasuga S, Sazuka T

Sci Rep. Vol. 7 2017.3

Sorghum Dw1, an agronomically important gene for lodging resistance, encodes a novel protein involved in cell proliferation. Reviewed

Yamaguchi M, Fujimoto H, Hirano K, Araki-Nakamura S, Ohmae-Shinohara K, Fujii A, Tsunashima M, Song XJ, Ito Y, Nagae R, Wu J, Mizuno H, Yonemaru J, Matsumoto T, Kitano H, Matsuoka M, Kasuga S, Sazuka T.

Sci Rep. 2016.6

Gibberellin deficiency pleiotropically induces culm bending in sorghum: an insight into sorghum semi-dwarf breeding.

Ordonio RL, Ito Y, Hatakeyama A, Ohmae-Shinohara K, Kasuga S, Tokunaga T, Mizuno H, Kitano H, Matsuoka M, Sazuka T.

Sci Rep. 2014.6

A rice tryptophan deficient dwarf mutant, tdd1, contains a reduced level of indole acetic acid and develops abnormal flowers and organless embryos Reviewed International journal

Sazuka Takashi, Kamiya Noriko, Nishimura Takeshi, Ohmae Kozue, Sato Yutaka, Imamura Kohei, Nagato Yasuo, Koshiba Tomokazu, Nagamura Yoshiaki, Ashikari Motoyuki, Kitano Hidemi, Matsuoka Makoto

PLANT JOURNAL Vol. 60 ( 2 ) page： 227 - 241 2009.10

Auxin Biosynthesis by the YUCCA Genes in Rice. Reviewed

Yamamoto Y, Kamiya N, Morinaka Y, Matsuoka M and Sazuka T.

Plant Physiol. Vol. 143 ( 3 ) page： 1362-1371 2007.3

Production and characterization of auxin-insensitive rice by overexpression of a mutagenized rice IAA protein. Reviewed

Nakamura A, Umemura I, Gomi K, Hasegawa Y, Kitano H, Sazuka T, Matsuoka M.

Plant J. Vol. 46 ( 2 ) page： 297-306 2006.4

A proteomic analysis of cyanobacterium, Anabaena sp. strain PCC7120 through two-dimensional gel electrophoresis and amino-terminal sequencing. Invited Reviewed

Sazuka T.

Photosynth Res. Vol. 78 ( 3 ) page： 279-291 2003.6

Towards a proteome project of cyanobacterium Synechocystis sp. strain PCC6803: Linking 130 protein spots with their respective genes. Invited Reviewed

Sazuka T, Ohara O

Electrophoresis Vol. 18 ( 8 ) page： 1252-1258 1997.8

ソルガムを培地成分とした黄麹菌の培養特性

本迫翔, 松本琴音, 鈴木智大, 若井暁, Kahar Prihardi, 森裕太郎, 佐塚隆志, 荻野千秋

日本生物工学会大会講演要旨集 Vol. 2024年 page： 94 - 94 2024.8

DAC農業の実現にむけた作物改良と評価 Invited Reviewed

矢野昌裕, 米丸淳一, 佐塚隆志, 小松晃, 木下俊則, 吉川直樹

育種学研究 Vol. 26 ( 1 ) page： 54 - 60 2024.4

Utilization of sweet sorghum juice as a carbon source for enhancement of itaconic acid production in engineered Corynebacterium glutamicum Reviewed International coauthorship

Elkasaby, T; Hanh, DD; Kahar, P; Kawaguchi, H; Sazuka, T; Kondo, A; Ogino, C

ENZYME AND MICROBIAL TECHNOLOGY Vol. 172 page： 110345 2024.1

Language：English Publishing type：Research paper (scientific journal) Publisher：Enzyme and Microbial Technology

Itaconic acid is a promising biochemical building block that can be used in polymer synthesis. Itaconic acid is currently produced in industry by the natural producer fungus Aspergillus terreus using glucose as a main carbon source. Most research for itaconic acid production using lignocellulosic-based carbon sources was carried out by A. terreus. Engineered Corynebacterium glutamicum strain which can grow in presence of fermentation inhibitors without effect on growth, was used for production of itaconic acid using sweet sorghum juice and bagasse sugar lysate (BSL). BSL contains many inhibitors unlike sorghum juice. C. glutamicum could grow in the media containing both types of lignocellulose-based carbon sources without showing any growth inhibition, however, sorghum juice was better in itaconic acid production than BSL. Different constructed strains of C. glutamicum were used for itaconic acid production, however, C. glutamicum ATCC 13032 pCH-Tad1optAdi1opt strain expressing Adi1/Tad1 genes (trans-pathway) from Ustilago maydis proved to be better in itaconic acid production giving final titer of 8.4 and 4.02 g/L using sweet sorghum juice and BSL as the sole carbon sources by fed-batch fermentation. Our study is the first for production of itaconic acid using sweet sorghum juice and BSL. The present study also proved that C. glutamicum can be used for enhancing itaconic acid production using lignocellulosic-based carbon sources.

DOI： 10.1016/j.enzmictec.2023.110345

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脱炭素社会構築に貢献する草本バイオマスの可能性. Invited

佐塚隆志

アグリバイオ Vol. 7 ( 6 ) page： 6 - 7 2023.6

ソルガムの雑種強勢メカニズムとバイオマスデザイニング Invited

橋本舜平、佐塚隆志

アグリバイオ Vol. 7 ( 6 ) page： 8 - 12 2023.6

Metabolic engineering for 4-aminophenylalanine production from lignocellulosic biomass by recombinant Escherichia coli. Reviewed

RSC Sustainability Vol. 1 ( 4 ) page： 1043 - 1054 2023.5

Analysis of photosynthetic characteristics by hierarchy in sorghum populations Invited

Abstracts of Meeting of the CSSJ 2023.3

Utilization of sweet sorghum juice as a carbon source for enhancement of itaconic acid production in engineered Corynebacterium glutamicum Reviewed International coauthorship

Taghreed Elkasaby T, Hanh DD, Kahar P, Kawaguchi H, Sazuka T, Kondo A, Ogino C.

Enzyme and Microbial Technology Vol. 172 ( 1 ) page： 110345. - 110345. 2023.1

Metabolic engineering for 4-aminophenylalanine production from lignocellulosic biomass by recombinant Escherichia coli

Hideo Kawaguchi, Shunsuke Masuo, Keiko Wakai, Naoki Takaya, Tomohisa Hasunma, Tatsuo Kaneko, Satoshi Okada, Takashi Sazuka, Chiaki Ogino, Akihiko Kondo

RSC Sustainability Vol. 1 ( 4 ) page： 1043 - 1054 2023

脱炭素社会構築への貢献を目標とした新しい作物育種 Invited

橋本舜平、佐塚隆志

作物研究 Vol. 67 page： 89 - 92 2022.10

Enhanced production of gamma-amino acid 3-amino-4-hydroxybenzoic acid by recombinant Corynebacterium glutamicum under oxygen limitation Reviewed International journal

Kawaguchi Hideo, Hasunuma Tomohisa, Ohnishi Yasuo, Sazuka Takashi, Kondo Akihiko, Ogino Chiaki

MICROBIAL CELL FACTORIES Vol. 20 ( 1 ) 2021.12

Complete and Draft Genome Sequences of Amino Acid-Producing Corynebacterium glutamicum Strains ATCC 21799 and ATCC 31831 and Their Genomic Islands Reviewed

Kawaguchi H, Sazuka T, Kondo A

Microbiol Resour Announc. Vol. 9 ( 32 ) 2020.8

Repeated ethanol fermentation from membrane-concentrated sweet sorghum juice using the flocculating yeast Saccharomyces cerevisiae F118 strain Reviewed

Wijaya H, Sasaki K, Kahar P, Yopi, Kawaguchi H, Sazuka T, Ogino C, Prasetya B, Kondo A

Vol. 265 page： 542 - 547 2018.10

Transcriptional switch for programmed cell death in pith parenchyma of sorghum stems Reviewed

Fujimoto M, Sazuka T, Oda Y, Kawahigashi H, Wu J, Takanashi H, Ohnishi T, Yoneda J, Ishimori M, Kajiya-Kanegae H, Hibara K, Ishizuna F, Ebine K, Ueda T, Tokunaga T, Iwata H, Matsumoto T, Kasuga S, Yonemaru J, Tsutsumi N

Vol. 115 ( 37 ) page： E8783 - E8792 2018.9

Effective usage of sorghum bagasse: Optimization of organosolv pretreatment using 25% 1-butanol and subsequent nanofiltration membrane separation Reviewed

Teramura H, Sasaki K, Oshima T, Kawaguchi H, Ogino C, Sazuka T, Kondo A

Vol. 252 page： 157 - 164 2018.3

Sucrose purification and repeated ethanol production from sugars remaining in sweet sorghum juice subjected to a membrane separation process Reviewed

Sasaki K, Tsuge Y, Kawaguchi H, Yasukawa M, Sasaki D, Sazuka T, Kamio E, Ogino C, Matsuyama H, Kondo A

Vol. 101 ( 15 ) page： 6007 - 6014 2017.8

Heap: a highly sensitive and accurate SNP detection tool for low-coverage high-throughput sequencing data Reviewed

Masaaki Kobayashi, Hajime Ohyanagi, Hideki Takanashi, Satomi Asano, Toru Kudo, Hiromi Kajiya-Kanegae, Atsushi J. Nagano, Hitoshi Tainaka, Tsuyoshi Tokunaga, Takashi Sazuka, Hiroyoshi Iwata, Nobuhiro Tsutsumi, Kentaro Yano

DNA Research Vol. 24 ( 4 ) page： 397 - 405 2017.8

Language：English Publishing type：Research paper (scientific journal) Publisher：Oxford University Press

Recent availability of large-scale genomic resources enables us to conduct so called genome-wide association studies (GWAS) and genomic prediction (GP) studies, particularly with next-generation sequencing (NGS) data. The effectiveness of GWAS and GP depends on not only their mathematical models, but the quality and quantity of variants employed in the analysis. In NGS single nucleotide polymorphism (SNP) calling, conventional tools ideally require more reads for higher SNP sensitivity and accuracy. In this study, we aimed to develop a tool, Heap, that enables robustly sensitive and accurate calling of SNPs, particularly with a low coverage NGS data, which must be aligned to the reference genome sequences in advance. To reduce false positive SNPs, Heap determines genotypes and calls SNPs at each site except for sites at the both ends of reads or containing a minor allele supported by only one read. Performance comparison with existing tools showed that Heap achieved the highest F-scores with low coverage (7X) restriction-site associated DNA sequencing reads of sorghum and rice individuals. This will facilitate cost-effective GWAS and GP studies in this NGS era. Code and documentation of Heap are freely available from https://github.com/meiji-bioinf/heap (29 March 2017, date last accessed) and our web site (http://bioinf.mind.meiji.ac.jp/lab/en/tools.html (29 March 2017, date last accessed)).

DOI： 10.1093/dnares/dsx012

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Screening of rice mutants with improved saccharification efficiency results in the identification of CONSTITUTIVE PHOTOMORPHOGENIC 1 and GOLD HULL AND INTERNODE 1 Reviewed

Hirano K, Masuda R, Takase W, Morinaka Y, Kawamura M, Takeuchi Y, Takagi H, Yaegashi H, Natsume S, Terauchi R, Kotake T, Matsushita Y, Sazuka T

Vol. 246 ( 1 ) page： 61 - 74 2017.7

Differences in glucose yield of residues from among varieties of rice, wheat, and sorghum after dilute acid pretreatment. Reviewed

Teramura, H., Sasaki, K., Kawaguchi, H., Matsuda, F., Kikuchi, J., Shirai, T., Sazuka, T., Yamasaki, M., Takumi S., Ogino, C., and Kondo, A.

Biosci Biotech Biochem. 2017.6

Differences in glucose yield of residues from among varieties of rice, wheat, and sorghum after dilute acid pretreatment Reviewed

Teramura H, Sasaki K, Kawaguchi H, Matsuda F, Kikuchi J, Shirai T, Sazuka T, Yamasaki M, Takumi S, Ogino C, Kondo A

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY Vol. 81 ( 8 ) page： 1650 - 1656 2017

Growth Properties and Biomass Production in the Hybrid C4 Crop Sorghum bicolor. Reviewed

Tazoe Y, Sazuka T, Yamaguchi M, Saito C, Ikeuchi M, Kanno K, Kojima S, Hirano K, Kitano H, Kasuga S, Endo T, Fukuda H, Makino A.

Plant Cell Physiol. 2016.5

Organosolv pretreatment of sorghum bagasse using a low concentration of hydrophobic solvents such as 1-butanol or 1-pentanol.

J Biosci Bioeng. Vol. 9 page： 27 2016.2

Nanofiltration concentration of extracellular glutathione produced by engineered Saccharomyces cerevisiae.

Sasaki K, Hara KY, Kawaguchi H, Sazuka T, Ogino C, Kondo A.

J Biosci Bioeng. 2016.1

O-18 Simultaneous saccharification and fermentation of lignocellulosic biomass for phenyllactate production by recombinant Escherichia coli Reviewed

川口,秀夫, 寺村,浩, 中村,聡子, 荻野,千秋, 原,清敬, 蓮沼,誠久, 老沼,研一, 高谷,直樹, 平野,恒, 佐塚,隆志, 北野,英己, 近藤,昭彦

Proceedings of the Conference on Biomass Science Vol. 11 page： 35 - 36 2016.1

Language：Japanese Publishing type：Research paper (international conference proceedings) Publisher：The Japan Institute of Energy

Sorghum bagasse pretreated with diluted acid, which was predominantly composed of glucan (59%) and xylan (7.2%), was used as a lignocellulosic feedstock to produce D-phenyllactic acid (PhLA) by a recombinant Escherichia coli strain expressing phenylpyruvate reductase from Wickerhamia fluorescens. Compared to filter paper hydrolysate, the PhLA yield was reduced by 35% during fermentation with enzymatic hydrolysate of sorghum bagasse as a carbon source, and metabolomics analysis revealed that intracellular levels of erythrose-4-phosphate and phosphoenolpyruvate and NAD(P)H regeneration for PhLA production from glucose markedly reduced. Compared to the separate hydrolysis and fermentation (SHF) with sorghum bagasse hydrolysate, simultaneous saccharification and fermentation (SSF) of sorghum bagasse under glucose limitation conditions yielded 4.8-fold more PhLA with less accumulation of eluted components of p-coumaric acid and aldehydes, which inhibited PhLA fermentation. These results suggest that gradual hydrolysis of sorghum bagasse during SSF reduces the accumulation of both glucose and fermentation inhibitors, collectively leading to increased PhLA yield.

DOI： 10.20550/jiebiomassronbun.11.0_35

CiNii Books

3-Amino-4-hydroxybenzoic acid production from sweet sorghum juice by recombinant Corynebacterium glutamicum Reviewed International journal

Hideo Kawaguchi, Kengo Sasaki, Kouji Uematsu, Yota Tsuge, Hiroshi Teramura, Naoko Okai, Sachiko Nakamura-Tsuruta, Yohei Katsuyama, Yoshinori Sugai, Yasuo Ohnishi, Ko Hirano, Takashi Sazuka, Chiaki Ogino, Akihiko Kondo

Bioresource Technology Vol. 198 page： 410 - 417 2015.12

Language：English Publishing type：Research paper (scientific journal) Publisher：ELSEVIER SCI LTD

The production of the bioplastic precursor 3-amino-4-hydroxybenzoic acid (3,4-AHBA) from sweet sorghum juice, which contains amino acids and the fermentable sugars sucrose, glucose and fructose, was assessed to address the limitations of producing bio-based chemicals from renewable feedstocks. Recombinant Corynebacterium glutamicum strain KT01 expressing griH and griI derived from Streptomyces griseus produced 3,4-AHBA from the sweet sorghum juice of cultivar SIL-05 at a final concentration (1.0 g l(-1)) that was 5-fold higher than that from pure sucrose. Fractionation of sweet sorghum juice by nanofiltration (NF) membrane separation (molecular weight cut-off 150) revealed that the NF-concentrated fraction, which contained the highest concentrations of amino acids, increased 3,4-AHBA production, whereas the NF-filtrated fraction inhibited 3,4-AHBA biosynthesis. Amino acid supplementation experiments revealed that leucine specifically enhanced 3,4-AHBA production by strain KT01. Taken together, these results suggest that sweet sorghum juice is a potentially suitable feedstock for 3,4-AHBA production by recombinant C. glutamicum. (C) 2015 Elsevier Ltd. All rights reserved.

DOI： 10.1016/j.biortech.2015.09.024

Nanofiltration concentration of extracellular glutathione produced by engineered Saccharomyces cerevisiae.

Sasaki K, Hara KY, Kawaguchi H, Sazuka T, Ogino C, Kondo A.

J Biosci Bioeng. 2015.6

Repeated ethanol production from sweet sorghum juice concentrated by membrane separation. Bioresour Technol.

Sasaki K, Tsuge Y, Sasaki D, Kawaguchi H, Sazuka T, Ogino C, Kondo A.

Bioresour Technol. 2015.6

Phenyllactic acid production by simultaneous saccharification and fermentation of pretreated sorghum bagasse.

Kawaguchi H, Teramura H, Uematsu K, Hara KY, Hasunuma T, Hirano K, Sazuka T, Kitano H, Tsuge Y, Kahar P, Niimi-Nakamura S, Oinuma K, Takaya N, Kasuga S, Ogino C, Kondo A.

Bioresour Technol. 2015.4

Repeated ethanol production from sweet sorghum juice concentrated by membrane separation.

Sasaki K, Tsuge Y, Sasaki D, Kawaguchi H, Sazuka T, Ogino C, Kondo A.

Bioresour Technol. Vol. 186 page： 351-355 2015.1

Increased ethanol production from sweet sorghum juice concentrated by a membrane separation process.

Sasaki K, Tsuge Y, Sasaki D, Teramura H, Wakai S, Kawaguchi H, Sazuka T, Ogino C, Kondo A.

Bioresour Technol. 2014.10

Phenyllactic acid production by simultaneous saccharification and fermentation of pretreated sorghum bagasse.

Kawaguchi H, Teramura H, Uematsu K, Hara KY, Hasunuma T, Hirano K, Sazuka T, Kitano H, Tsuge Y, Kahar P, Niimi-Nakamura S, Oinuma K, Takaya N, Kasuga S, Ogino C, Kondo A.

Bioresour Technol. 2014.4

From Genome to Breeding: Challenge of Genome-Assisted Breeding of Sorghum.

Sazuka T and Iwata H.

J. Jpn Inst. Enregy Vol. 93 page： 429-435 2014

Thioredoxin h regulates calcium dependent protein kinases in plasma membranes.

Ueoka-Nakanishi, H., Sazuka, T., Nakanishi, Y., Maeshima, M., Mori, H. and Hisabori, T.

FEBS J. Vol. 280 page： 3220-3231 2013

A high-throughput phenotyping system supporting genomic breeding of crop plants International journal

Iwata H., Kobayashi M., Ohyanagi H., Yano K., Sazuka T., Tokunaga T., Tsutsumi N.

Proceedings of the SICE Annual Conference page： 1778 - 1779 2013

Language：English Publishing type：Research paper (international conference proceedings) Publisher：Proceedings of the SICE Annual Conference

High-throughput technologies for determining DNA sequence variations have enabled new genomics-based breeding strategies, such as genomic selection. While genomics technologies are making rapid progress in throughput and cost efficiencies, technologies for measuring plant phenotypes, i.e., "phenotyping", remain inefficient. Phenotyping is now the most time-consuming and error-prone step in genomic-based breeding strategies. To measure plant phenotypes with high efficiency and accuracy, we are developing a high-throughput phenotyping system. The system consists of two main parts: The plant management system using radio-frequency identification device (RFID) tags and the data collection system connected to multiple measuring devices via Bluetooth. The first system enables us to link plants to data on the Internet and to keep records of cultivation management. The second system enables us to collect data measured by multiple devices via Bluetooth connections. With this system, we can aggregate various types of measurement data in an efficient and precise manner. High-throughput phenotyping systems lead to efficient and accurate data collection and will strongly support genomic breeding in crop plants.

Scopus

Mapping and characterization of the rice gene, CLUB-SHAPED EMBRYO3, CLE3. Reviewed

Imamura K, Nagato Y, Matsuoka M, Kitano H, Sazuka T.

Rice Gen. Newslett. Vol. 25 ( 1 ) page： 27-29 2010.5

A rice tryptophan deficient dwarf mutant, tdd1, contains a reduced level of indole acetic acid and develops abnormal flowers and organless embryos. Reviewed

Sazuka T, Kamiya N, Nishimura T, Ohmae K, Sato Y, Imamura K, Nagato Y, Koshiba T, Nagamura Y, Ashikari M, Kitano H, Matsuoka M.

Plant J. Vol. 60 ( 2 ) page： 227-241 2009.10

Mapping and Analysis of the CLUB-SHAPED EMBRYO1, CLE1. Reviewed

Vol. 24 ( 1 ) page： 22-24 2008.9

Possible thioredoxin targets of signaling molecules in plasma membranes Reviewed International journal

Ueoka-Nakanishi Hanayo, Sazuka Takashi, Maeshima Masayoshi, Hisabori Toru

PLANT AND CELL PHYSIOLOGY Vol. 48 page： S41 - S41 2007

Analysis of club-shaped embryol, a loss of function mutant of rice DICER-LIKE1

Kohei Imamura, Hanako Imai, Makoto Matsuoka, Hidemi Kitano, Takashi Sazuka

PLANT AND CELL PHYSIOLOGY Vol. 48 page： S36 - S36 2007

The rice SPINDLY gene functions as a negative regulator of gibberellin signaling by controlling the suppressive function of the DELLA protein, SLR1, and modulating brassinosteroid synthesis. Reviewed

Shimada A, Ueguchi-Tanaka M, Sakamoto T, Fujioka S, Takatsuto S, Yoshida S, Sazuka T, Ashikari, M, Matsuoka M.

Plant J. Vol. 48 ( 3 ) page： 390-402 2006.11

Exploring the thioredoxin targets in plant membrane proteins

H Ueoka-Nakanishi, M Maeshima, T Sazuka, T Hisabori

PLANT AND CELL PHYSIOLOGY Vol. 47 page： S78 - S78 2006

The rice mutant dwarf bamboo shoot 1: A leaky mutant of the NACK-type kinesin-like gene can initiate organ primordia but not organ development Reviewed International journal

Sazuka T, Aichi I, Kawai T, Matsuo N, Kitano H, Matsuoka M

PLANT AND CELL PHYSIOLOGY Vol. 46 ( 12 ) page： 1934 - 1943 2005.12

Analysis of the rice mutant dwarf and gladius leaf 1. Aberrant katanin-mediated microtubule organization causes up-regulation of gibberellin biosynthetic genes independently of gibberellin signaling Reviewed International journal

Komorisono M, Ueguchi-Tanaka M, Aichi I, Hasegawa Y, Ashikari M, Kitano H, Matsuoka M, Sazuka T

PLANT PHYSIOLOGY Vol. 138 ( 4 ) page： 1982 - 1993 2005.8

Isolation of intact vacuoles and proteomic analysis of tonoplast from suspension-cultured cells of Arabidopsis thaliana. Reviewed

Shimaoka T, Ohnishi M, Sazuka T, Mitsuhashi N, Hara-Nishimura I, Shimazaki KI, Maeshima M, Yokota A, Tomizawa KI, Mimura T.

Plant Cell Physiol. Vol. 45 ( 6 ) page： 672-683 2004.6

A proteomic approach to identification of transmembrane proteins and membrane-anchored proteins of Arabidopsis thaliana by peptide Sequencing Reviewed International journal

Sazuka T, Keta S, Shiratake K, Yamaki S, Shibata D

DNA RESEARCH Vol. 11 ( 2 ) page： 101 - 113 2004.4

SigC, the group 2 sigma factor of RNA polymerase, contributes to the late-stage gene expression and nitrogen promoter recognition in the Cyanobacterium Synechocystis sp. strain PCC 6803. Reviewed

Vol. 68 ( 3 ) page： 477-487 2004.3

Analysis of dwarf and abnormal leaf morphology 1 (dal1) in rice Invited Reviewed International journal

T Sazuka, M Komorisono, M Ueguchi-Tanaka, H Kitano, M Ashikari, M Matsuoka

PLANT AND CELL PHYSIOLOGY Vol. 45 page： S113 - S113 2004

The proteome analysis of the Cyanobacterium Anabaena sp. strain PCC 7120 and classification of the peptides detectable by MALDI-TOF/MS.

Ojima N, Sazuka T, Yamaguchi M, Kuriki T, Akinaga N, Ando E.

51st ASMS Conference on Mass Spectrometry and Allied Topics page： 507 2003

A proteomic approach to identification of transmembrane proteins and membrane-anchored proteins of Arabidopsis thaliana Reviewed International coauthorship International journal

T Sazuka, S Keta, K Shiratake, S Yamaki, D Shibata

PLANT AND CELL PHYSIOLOGY Vol. 44 page： S18 - S18 2003

Proteomic analysis of the cyanobacterium Anabaena sp strain PCC7120 with two-dimensional gel electrophoresis and amino-terminal sequencing Reviewed International journal

Sazuka T

PHOTOSYNTHESIS RESEARCH Vol. 78 ( 3 ) page： 279 - 291 2003

Isolation and characterization of intact vacuoles from suspension-cultured Arabidopsis cells

M Ohnishi, N Mitsuhashi, Y Sekiguchi, T Sazuka, K Shimazaki, Nishimura, I

PLANT AND CELL PHYSIOLOGY Vol. 44 page： S85 - S85 2003

Proteomics of Arabidopsis thaliana: a comprehensive analysis of the strongly hydrophobic membrane proteins Reviewed

T Sazuka, S Keta, K Shiratake, S Yamaki, D Shibata

PLANT AND CELL PHYSIOLOGY Vol. 43 page： S207 - S207 2002

Prediction of the coding sequences of unidentified human genes. II. The coding sequences of 40 new genes (KIAA0041-KIAA0080) deduced by analysis of cDNA clones from human cell line KG-1 (supplement). Reviewed

N. Nomura, T. Nagase, N. Miyajima, T. Sazuka, A. Tanaka, S. Sato, N. Seki, Y. Kawarabayasi, K. Ishikawa, S. Tabata

DNA research Vol. 1 page： 251 - 262 1994.12

Prediction of the coding sequences of unidentified human genes. I. The coding sequences of 40 new genes (KIAA0001-KIAA0040) deduced by analysis of randomly sampled cDNA clones from human immature myeloid cell line KG-1 (supplement).

N. Nomura, N. Miyajima, T. Sazuka, A. Tanaka, Y. Kawarabayasi, S. Sato, T. Nagase, N. Seki, K. Ishikawa, S. Tabata

DNA research : an international journal for rapid publication of reports on genes and genomes Vol. 1 ( 1 ) page： 47 - 56 1994

Identification of a developmentally regulated gene in the mouse central nervous system which encodes a novel proline rich protein Reviewed

Takashi Sazuka, Yasuhiro Tomooka, Sandeep Kathju, Yoji Ikawa, Makoto Noda, Sharad Kumar

BBA - Gene Structure and Expression Vol. 1132 ( 3 ) page： 240 - 248 1992.10

Language：English Publishing type：Research paper (scientific journal)

A full length cDNA whose corresponding mRNA is down-regulated during the mouse embryonic brain development was isolated. The cDNA contains a single long open reading frame which could encode a protein with relative molecular mass of 41 kDa. The predicted gene product contains long stretches of prolines towards the NH2-terminus, followed by a leucine/proline rich region. The cDNA probe detected a number of mRNA species in Northern blot analysis. The reverse transcriptase-polymerase chain reaction analysis of mRNA from adult mouse tissues indicated that heart and testis expressed this gene (named NDPP-1) at relatively high levels, while lower levels of mRNA were detected in a number of other tissues. Expression of NDPP-1 was also detected in embryonic carcinoma and pheochromocytoma cell lines, but not in fibroblasts. The cDNA hybridized to genomic DNA from several vertebrates species in Southern blot analysis indicating interspecies conservation of this gene. The interesting pattern of expression of the NDPP-1 gene during mouse brain development and the structure of its putative protein product indicate that this gene may play an important biological role in the development of mouse central nervous system. © 1992.

DOI： 10.1016/0167-4781(92)90156-T

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<SAZUKA, Takashi>

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