Faculty Profiles - SAZUKA, Takashi

Papers - SAZUKA, Takashi

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An analysis of sugary endosperm in sorghum: Characterization of mutant phenotypes depending on alleles of the corresponding starch debranching enzyme Reviewed

Shumpei Hashimoto, Satoshi Okada, Satoko Araki-Nakamura, Kozue Ohmae-Shinohara, Kotaro Miura, Hideo Kawaguchi, Chiaki Ogino, Shigemitsu Kasuga, Takashi Sazuka

Frontiers in Plant Science Vol. 14 page： 1114935 2023.2

Authorship：Last author,　Corresponding author Language：English Publishing type：Research paper (scientific journal) Publisher：Frontiers Media SA

Sorghum is the fifth most important cereal crop. Here we performed molecular genetic analyses of the ‘SUGARY FETERITA’ (SUF) variety, which shows typical sugary endosperm traits (e.g., wrinkled seeds, accumulation of soluble sugars, and distorted starch). Positional mapping indicated that the corresponding gene was located on the long arm of chromosome 7. Within the candidate region of 3.4 Mb, a sorghum ortholog for maize Su1 (SbSu) encoding a starch debranching enzyme ISA1 was found. Sequencing analysis of SbSu in SUF uncovered nonsynonymous single nucleotide polymorphisms (SNPs) in the coding region, containing substitutions of highly conserved amino acids. Complementation of the rice sugary-1 (osisa1) mutant line with the SbSu gene recovered the sugary endosperm phenotype. Additionally, analyzing mutants obtained from an EMS-induced mutant panel revealed novel alleles with phenotypes showing less severe wrinkles and higher Brix scores. These results suggested that SbSu was the corresponding gene for the sugary endosperm. Expression profiles of starch synthesis genes during the grain-filling stage demonstrated that a loss-of-function of SbSu affects the expression of most starch synthesis genes and revealed the fine-tuned gene regulation in the starch synthetic pathway in sorghum. Haplotype analysis using 187 diverse accessions from a sorghum panel revealed the haplotype of SUF showing severe phenotype had not been used among the landraces and modern varieties. Thus, weak alleles (showing sweet and less severe wrinkles), such as in the abovementioned EMS-induced mutants, are more valuable for grain sorghum breeding. Our study suggests that more moderate alleles (e.g. produced by genome editing) should be beneficial for improving grain sorghum.

DOI： 10.3389/fpls.2023.1114935

Web of Science

Scopus

PubMed

ソルガムの雑種強勢メカニズムとバイオマスデザイニング Invited Reviewed

橋本舜平、佐塚隆志

アグリバイオ Vol. 7 ( 6 ) page： 8 - 12 2023.6

The dominance model for heterosis explains culm length genetics in a hybrid sorghum variety Reviewed International journal

Hashimoto Shumpei, Wake Tatsuro, Nakamura Haruki, Minamiyama Masaki, Araki-Nakamura Satoko, Ohmae-Shinohara Kozue, Koketsu Eriko, Okamura Shinnosuke, Miura Kotaro, Kawaguchi Hideo, Kasuga Shigemitsu, Sazuka Takashi

SCIENTIFIC REPORTS Vol. 11 ( 1 ) 2021.2

Sorghum DW1 positively regulates brassinosteroid signaling by inhibiting the nuclear localization of BRASSINOSTEROID INSENSITIVE 2 Reviewed

Hirano K, Kawamura M, Araki-Nakamura S, Fujimoto H, Ohmae-Shinohara K, Yamaguchi M, Fujii A, Sasaki H, Kasuga S, Sazuka T

Sci Rep. Vol. 7 2017.3

Sorghum Dw1, an agronomically important gene for lodging resistance, encodes a novel protein involved in cell proliferation. Reviewed

Yamaguchi M, Fujimoto H, Hirano K, Araki-Nakamura S, Ohmae-Shinohara K, Fujii A, Tsunashima M, Song XJ, Ito Y, Nagae R, Wu J, Mizuno H, Yonemaru J, Matsumoto T, Kitano H, Matsuoka M, Kasuga S, Sazuka T.

Sci Rep. 2016.6

Gibberellin deficiency pleiotropically induces culm bending in sorghum: an insight into sorghum semi-dwarf breeding.

Ordonio RL, Ito Y, Hatakeyama A, Ohmae-Shinohara K, Kasuga S, Tokunaga T, Mizuno H, Kitano H, Matsuoka M, Sazuka T.

Sci Rep. 2014.6

A rice tryptophan deficient dwarf mutant, tdd1, contains a reduced level of indole acetic acid and develops abnormal flowers and organless embryos Reviewed International journal

Sazuka Takashi, Kamiya Noriko, Nishimura Takeshi, Ohmae Kozue, Sato Yutaka, Imamura Kohei, Nagato Yasuo, Koshiba Tomokazu, Nagamura Yoshiaki, Ashikari Motoyuki, Kitano Hidemi, Matsuoka Makoto

PLANT JOURNAL Vol. 60 ( 2 ) page： 227 - 241 2009.10

Auxin Biosynthesis by the YUCCA Genes in Rice. Reviewed

Yamamoto Y, Kamiya N, Morinaka Y, Matsuoka M and Sazuka T.

Plant Physiol. Vol. 143 ( 3 ) page： 1362-1371 2007.3

Production and characterization of auxin-insensitive rice by overexpression of a mutagenized rice IAA protein. Reviewed

Nakamura A, Umemura I, Gomi K, Hasegawa Y, Kitano H, Sazuka T, Matsuoka M.

Plant J. Vol. 46 ( 2 ) page： 297-306 2006.4

A proteomic analysis of cyanobacterium, Anabaena sp. strain PCC7120 through two-dimensional gel electrophoresis and amino-terminal sequencing. Invited Reviewed

Sazuka T.

Photosynth Res. Vol. 78 ( 3 ) page： 279-291 2003.6

Towards a proteome project of cyanobacterium Synechocystis sp. strain PCC6803: Linking 130 protein spots with their respective genes. Invited Reviewed

Sazuka T, Ohara O

Electrophoresis Vol. 18 ( 8 ) page： 1252-1258 1997.8

ソルガムを培地成分とした黄麹菌の培養特性

本迫翔, 松本琴音, 鈴木智大, 若井暁, Kahar Prihardi, 森裕太郎, 佐塚隆志, 荻野千秋

日本生物工学会大会講演要旨集 Vol. 2024年 page： 94 - 94 2024.8

DAC農業の実現にむけた作物改良と評価 Invited Reviewed

矢野昌裕, 米丸淳一, 佐塚隆志, 小松晃, 木下俊則, 吉川直樹

育種学研究 Vol. 26 ( 1 ) page： 54 - 60 2024.4

Utilization of sweet sorghum juice as a carbon source for enhancement of itaconic acid production in engineered Corynebacterium glutamicum Reviewed International coauthorship

Elkasaby, T; Hanh, DD; Kahar, P; Kawaguchi, H; Sazuka, T; Kondo, A; Ogino, C

ENZYME AND MICROBIAL TECHNOLOGY Vol. 172 page： 110345 2024.1

Language：English Publishing type：Research paper (scientific journal) Publisher：Enzyme and Microbial Technology

Itaconic acid is a promising biochemical building block that can be used in polymer synthesis. Itaconic acid is currently produced in industry by the natural producer fungus Aspergillus terreus using glucose as a main carbon source. Most research for itaconic acid production using lignocellulosic-based carbon sources was carried out by A. terreus. Engineered Corynebacterium glutamicum strain which can grow in presence of fermentation inhibitors without effect on growth, was used for production of itaconic acid using sweet sorghum juice and bagasse sugar lysate (BSL). BSL contains many inhibitors unlike sorghum juice. C. glutamicum could grow in the media containing both types of lignocellulose-based carbon sources without showing any growth inhibition, however, sorghum juice was better in itaconic acid production than BSL. Different constructed strains of C. glutamicum were used for itaconic acid production, however, C. glutamicum ATCC 13032 pCH-Tad1optAdi1opt strain expressing Adi1/Tad1 genes (trans-pathway) from Ustilago maydis proved to be better in itaconic acid production giving final titer of 8.4 and 4.02 g/L using sweet sorghum juice and BSL as the sole carbon sources by fed-batch fermentation. Our study is the first for production of itaconic acid using sweet sorghum juice and BSL. The present study also proved that C. glutamicum can be used for enhancing itaconic acid production using lignocellulosic-based carbon sources.

DOI： 10.1016/j.enzmictec.2023.110345

Web of Science

Scopus

PubMed

脱炭素社会構築に貢献する草本バイオマスの可能性. Invited

佐塚隆志

アグリバイオ Vol. 7 ( 6 ) page： 6 - 7 2023.6

ソルガムの雑種強勢メカニズムとバイオマスデザイニング Invited

橋本舜平、佐塚隆志

アグリバイオ Vol. 7 ( 6 ) page： 8 - 12 2023.6

Metabolic engineering for 4-aminophenylalanine production from lignocellulosic biomass by recombinant Escherichia coli. Reviewed

RSC Sustainability Vol. 1 ( 4 ) page： 1043 - 1054 2023.5

Analysis of photosynthetic characteristics by hierarchy in sorghum populations Invited

Abstracts of Meeting of the CSSJ 2023.3

Utilization of sweet sorghum juice as a carbon source for enhancement of itaconic acid production in engineered Corynebacterium glutamicum Reviewed International coauthorship

Taghreed Elkasaby T, Hanh DD, Kahar P, Kawaguchi H, Sazuka T, Kondo A, Ogino C.

Enzyme and Microbial Technology Vol. 172 ( 1 ) page： 110345. - 110345. 2023.1

Metabolic engineering for 4-aminophenylalanine production from lignocellulosic biomass by recombinant Escherichia coli

Hideo Kawaguchi, Shunsuke Masuo, Keiko Wakai, Naoki Takaya, Tomohisa Hasunma, Tatsuo Kaneko, Satoshi Okada, Takashi Sazuka, Chiaki Ogino, Akihiko Kondo

RSC Sustainability Vol. 1 ( 4 ) page： 1043 - 1054 2023

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Papers - SAZUKA, Takashi