Updated on 2024/09/27

写真a

 
SAZUKA, Takashi
 
Organization
Bioscience and Biotechnology Center Professor
Graduate School
Graduate School of Bioagricultural Sciences
Title
Professor

Degree 1

  1. 理学博士 （ 1993.3   筑波大学 ） 

To the head of Degree.▲

Research Interests 10

  1. ソルガム

  2. 雑種強勢

  3. バイオマス

  4. 高糖性

  5. ゲノム

  6. 育種

  7. バイオリファイナリー

  8. バイオエタノール

  9. イネ

  10. 発生

To the head of Research Interests.▲

Research Areas 2

  1. Others / Others  / Plant Genomics and Breeding

  2. Life Science / Plant molecular biology and physiology

To the head of Research Areas.▲

Current Research Project and SDGs 2

  1. 雑種強勢の原理解明によるバイオマス技術革新

  2. 野生ソルガムを活用した新規DAC型作物の創出

To the head of Current Research Project and SDGs.▲

Research History 1

  1. 財団法人 かずさＤＮＡ研究所   研究員

    1993.4 - 2003.4

    　 More details

    Country：Japan

To the head of Research History.▲

Education 2

  1. University of Tsukuba

    - 1993

    　 More details

    Country： Japan

  2. University of Tsukuba

    - 1988

    　 More details

    Country： Japan

To the head of Education.▲

Professional Memberships 6

  1. 日本育種学会

  2. 日本植物生理学会

  3. 日本分子生物学会

  4. 日本育種学会

  5. 日本植物生理学会

  6. 日本分子生物学会

▼display all

To the head of Professional Memberships.▲

Committee Memberships 2

  1. 日本育種学会   代議員  

    2023.4   

    　 More details

    Committee type：Academic society

  2. Proteomics   Editor  

    2001.4 - 2003.3   

To the head of Committee Memberships.▲
 

Papers 65

  1. An analysis of sugary endosperm in sorghum: Characterization of mutant phenotypes depending on alleles of the corresponding starch debranching enzyme Reviewed

    Shumpei Hashimoto, Satoshi Okada, Satoko Araki-Nakamura, Kozue Ohmae-Shinohara, Kotaro Miura, Hideo Kawaguchi, Chiaki Ogino, Shigemitsu Kasuga, Takashi Sazuka

    Frontiers in Plant Science   Vol. 14   page： 1114935   2023.2

    　More details

    Authorship：Last author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Frontiers Media SA  

    Sorghum is the fifth most important cereal crop. Here we performed molecular genetic analyses of the ‘SUGARY FETERITA’ (SUF) variety, which shows typical sugary endosperm traits (e.g., wrinkled seeds, accumulation of soluble sugars, and distorted starch). Positional mapping indicated that the corresponding gene was located on the long arm of chromosome 7. Within the candidate region of 3.4 Mb, a sorghum ortholog for maize Su1 (SbSu) encoding a starch debranching enzyme ISA1 was found. Sequencing analysis of SbSu in SUF uncovered nonsynonymous single nucleotide polymorphisms (SNPs) in the coding region, containing substitutions of highly conserved amino acids. Complementation of the rice sugary-1 (osisa1) mutant line with the SbSu gene recovered the sugary endosperm phenotype. Additionally, analyzing mutants obtained from an EMS-induced mutant panel revealed novel alleles with phenotypes showing less severe wrinkles and higher Brix scores. These results suggested that SbSu was the corresponding gene for the sugary endosperm. Expression profiles of starch synthesis genes during the grain-filling stage demonstrated that a loss-of-function of SbSu affects the expression of most starch synthesis genes and revealed the fine-tuned gene regulation in the starch synthetic pathway in sorghum. Haplotype analysis using 187 diverse accessions from a sorghum panel revealed the haplotype of SUF showing severe phenotype had not been used among the landraces and modern varieties. Thus, weak alleles (showing sweet and less severe wrinkles), such as in the abovementioned EMS-induced mutants, are more valuable for grain sorghum breeding. Our study suggests that more moderate alleles (e.g. produced by genome editing) should be beneficial for improving grain sorghum.

    DOI： 10.3389/fpls.2023.1114935

    Web of Science

    Scopus

    PubMed

  2. ソルガムの雑種強勢メカニズムとバイオマスデザイニング Invited Reviewed

    橋本舜平、佐塚隆志

    アグリバイオ   Vol. 7 ( 6 ) page： 8 - 12   2023.6

    　More details

    Authorship：Last author,　Corresponding author   Language：Japanese   Publishing type：Research paper (scientific journal)  

  3. The dominance model for heterosis explains culm length genetics in a hybrid sorghum variety Reviewed International journal

    Hashimoto Shumpei, Wake Tatsuro, Nakamura Haruki, Minamiyama Masaki, Araki-Nakamura Satoko, Ohmae-Shinohara Kozue, Koketsu Eriko, Okamura Shinnosuke, Miura Kotaro, Kawaguchi Hideo, Kasuga Shigemitsu, Sazuka Takashi

    SCIENTIFIC REPORTS   Vol. 11 ( 1 )   2021.2

    　More details

    Authorship：Last author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1038/s41598-021-84020-3

    Web of Science

  4. Sorghum DW1 positively regulates brassinosteroid signaling by inhibiting the nuclear localization of BRASSINOSTEROID INSENSITIVE 2 Reviewed

    Hirano K, Kawamura M, Araki-Nakamura S, Fujimoto H, Ohmae-Shinohara K, Yamaguchi M, Fujii A, Sasaki H, Kasuga S, Sazuka T

    Sci Rep.   Vol. 7   2017.3

    　More details

    Authorship：Last author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1038/s41598-017-00096-w

    Web of Science

  5. Sorghum Dw1, an agronomically important gene for lodging resistance, encodes a novel protein involved in cell proliferation. Reviewed

    Yamaguchi M, Fujimoto H, Hirano K, Araki-Nakamura S, Ohmae-Shinohara K, Fujii A, Tsunashima M, Song XJ, Ito Y, Nagae R, Wu J, Mizuno H, Yonemaru J, Matsumoto T, Kitano H, Matsuoka M, Kasuga S, Sazuka T.

    Sci Rep.     2016.6

    　More details

    Authorship：Last author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1038/srep28366.

  6. Gibberellin deficiency pleiotropically induces culm bending in sorghum: an insight into sorghum semi-dwarf breeding.

    Ordonio RL, Ito Y, Hatakeyama A, Ohmae-Shinohara K, Kasuga S, Tokunaga T, Mizuno H, Kitano H, Matsuoka M, Sazuka T.

    Sci Rep.     2014.6

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1038/srep05287.

  7. A rice tryptophan deficient dwarf mutant, tdd1, contains a reduced level of indole acetic acid and develops abnormal flowers and organless embryos Reviewed International journal

    Sazuka Takashi, Kamiya Noriko, Nishimura Takeshi, Ohmae Kozue, Sato Yutaka, Imamura Kohei, Nagato Yasuo, Koshiba Tomokazu, Nagamura Yoshiaki, Ashikari Motoyuki, Kitano Hidemi, Matsuoka Makoto

    PLANT JOURNAL   Vol. 60 ( 2 ) page： 227 - 241   2009.10

    　More details

    Authorship：Last author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1111/j.1365-313X.2009.03952.x

    Web of Science

  8. Auxin Biosynthesis by the YUCCA Genes in Rice. Reviewed

    Yamamoto Y, Kamiya N, Morinaka Y, Matsuoka M and Sazuka T.

    Plant Physiol.   Vol. 143 ( 3 ) page： 1362-1371   2007.3

    　More details

    Authorship：Last author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

  9. Production and characterization of auxin-insensitive rice by overexpression of a mutagenized rice IAA protein. Reviewed

    Nakamura A, Umemura I, Gomi K, Hasegawa Y, Kitano H, Sazuka T, Matsuoka M.

    Plant J.   Vol. 46 ( 2 ) page： 297-306   2006.4

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

  10. A proteomic analysis of cyanobacterium, Anabaena sp. strain PCC7120 through two-dimensional gel electrophoresis and amino-terminal sequencing. Invited Reviewed

    Sazuka T.

    Photosynth Res.   Vol. 78 ( 3 ) page： 279-291   2003.6

    　More details

    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

  11. Towards a proteome project of cyanobacterium Synechocystis sp. strain PCC6803: Linking 130 protein spots with their respective genes. Invited Reviewed

    Sazuka T, Ohara O

    Electrophoresis   Vol. 18 ( 8 ) page： 1252-1258   1997.8

    　More details

    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

  12. DAC農業の実現にむけた作物改良と評価 Invited Reviewed

    矢野昌裕, 米丸淳一, 佐塚隆志, 小松晃, 木下俊則, 吉川直樹

    育種学研究   Vol. 26 ( 1 ) page： 54 - 60   2024.4

    　More details

    Language：Japanese   Publishing type：Research paper (international conference proceedings)  

    Research data storage URL： https://www.jstage.jst.go.jp/article/jsbbr/26/1/26_26.W01/_article/-char/ja

  13. Utilization of sweet sorghum juice as a carbon source for enhancement of itaconic acid production in engineered<i> Corynebacterium</i><i> glutamicum</i> Reviewed International coauthorship

    Elkasaby, T; Hanh, DD; Kahar, P; Kawaguchi, H; Sazuka, T; Kondo, A; Ogino, C

    ENZYME AND MICROBIAL TECHNOLOGY   Vol. 172   page： 110345   2024.1

    　More details

    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Enzyme and Microbial Technology  

    Itaconic acid is a promising biochemical building block that can be used in polymer synthesis. Itaconic acid is currently produced in industry by the natural producer fungus Aspergillus terreus using glucose as a main carbon source. Most research for itaconic acid production using lignocellulosic-based carbon sources was carried out by A. terreus. Engineered Corynebacterium glutamicum strain which can grow in presence of fermentation inhibitors without effect on growth, was used for production of itaconic acid using sweet sorghum juice and bagasse sugar lysate (BSL). BSL contains many inhibitors unlike sorghum juice. C. glutamicum could grow in the media containing both types of lignocellulose-based carbon sources without showing any growth inhibition, however, sorghum juice was better in itaconic acid production than BSL. Different constructed strains of C. glutamicum were used for itaconic acid production, however, C. glutamicum ATCC 13032 pCH-Tad1optAdi1opt strain expressing Adi1/Tad1 genes (trans-pathway) from Ustilago maydis proved to be better in itaconic acid production giving final titer of 8.4 and 4.02 g/L using sweet sorghum juice and BSL as the sole carbon sources by fed-batch fermentation. Our study is the first for production of itaconic acid using sweet sorghum juice and BSL. The present study also proved that C. glutamicum can be used for enhancing itaconic acid production using lignocellulosic-based carbon sources.

    DOI： 10.1016/j.enzmictec.2023.110345

    Web of Science

    Scopus

    PubMed

  14. 脱炭素社会構築に貢献する草本バイオマスの可能性. Invited

    佐塚隆志

    アグリバイオ   Vol. 7 ( 6 ) page： 6 - 7   2023.6

    　More details

    Authorship：Lead author,　Last author,　Corresponding author   Publishing type：Part of collection (book)  

  15. ソルガムの雑種強勢メカニズムとバイオマスデザイニング Invited

    橋本舜平、佐塚隆志

    アグリバイオ   Vol. 7 ( 6 ) page： 8 - 12   2023.6

    　More details

    Authorship：Last author,　Corresponding author   Publishing type：Part of collection (book)  

  16. Metabolic engineering for 4-aminophenylalanine production from lignocellulosic biomass by recombinant Escherichia coli. Reviewed

    RSC Sustainability   Vol. 1 ( 4 ) page： 1043 - 1054   2023.5

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

  17. Analysis of photosynthetic characteristics by hierarchy in <i>sorghum</i> populations Invited

    Abstracts of Meeting of the CSSJ     2023.3

    　More details

    Publishing type：Research paper (conference, symposium, etc.)  

  18. Utilization of sweet sorghum juice as a carbon source for enhancement of itaconic acid production in engineered Corynebacterium glutamicum Reviewed International coauthorship

    Taghreed Elkasaby T, Hanh DD, Kahar P, Kawaguchi H, Sazuka T, Kondo A, Ogino C.

    Enzyme and Microbial Technology   Vol. 172 ( 1 ) page： 110345. - 110345.   2023.1

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： https://doi.org/10.1016/j.enzmictec.2023.110345

  19. Metabolic engineering for 4-aminophenylalanine production from lignocellulosic biomass by recombinant <i>Escherichia coli</i>

    Hideo Kawaguchi, Shunsuke Masuo, Keiko Wakai, Naoki Takaya, Tomohisa Hasunma, Tatsuo Kaneko, Satoshi Okada, Takashi Sazuka, Chiaki Ogino, Akihiko Kondo

    RSC Sustainability   Vol. 1 ( 4 ) page： 1043 - 1054   2023

    　More details

    Publishing type：Research paper (scientific journal)   Publisher：Royal Society of Chemistry (RSC)  

    4-Aminophenylalanine as a diamine monomer to synthesize unique biopolyimides was produced from lignocellulosic biomass by microbial fermentation. Fermentation inhibitors present in lignocellulosic hydrolysate of sorghum bagasse were identified.

    DOI： 10.1039/d3su00053b

    Scopus

  20. 脱炭素社会構築への貢献を目標とした新しい作物育種 Invited

    橋本舜平、佐塚隆志

    作物研究   Vol. 67   page： 89 - 92   2022.10

    　More details

    Authorship：Last author,　Corresponding author   Language：Japanese   Publishing type：Research paper (scientific journal)  

  21. Enhanced production of gamma-amino acid 3-amino-4-hydroxybenzoic acid by recombinant Corynebacterium glutamicum under oxygen limitation Reviewed International journal

    Kawaguchi Hideo, Hasunuma Tomohisa, Ohnishi Yasuo, Sazuka Takashi, Kondo Akihiko, Ogino Chiaki

    MICROBIAL CELL FACTORIES   Vol. 20 ( 1 )   2021.12

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1186/s12934-021-01714-z

    Web of Science

  22. Complete and Draft Genome Sequences of Amino Acid-Producing Corynebacterium glutamicum Strains ATCC 21799 and ATCC 31831 and Their Genomic Islands Reviewed

    Kawaguchi H, Sazuka T, Kondo A

    Microbiol Resour Announc.   Vol. 9 ( 32 )   2020.8

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1128/MRA.00430-20

    Web of Science

  23. Repeated ethanol fermentation from membrane-concentrated sweet sorghum juice using the flocculating yeast Saccharomyces cerevisiae F118 strain Reviewed

    Wijaya H, Sasaki K, Kahar P, Yopi, Kawaguchi H, Sazuka T, Ogino C, Prasetya B, Kondo A

      Vol. 265   page： 542 - 547   2018.10

    　More details

  24. Transcriptional switch for programmed cell death in pith parenchyma of sorghum stems Reviewed

    Fujimoto M, Sazuka T, Oda Y, Kawahigashi H, Wu J, Takanashi H, Ohnishi T, Yoneda J, Ishimori M, Kajiya-Kanegae H, Hibara K, Ishizuna F, Ebine K, Ueda T, Tokunaga T, Iwata H, Matsumoto T, Kasuga S, Yonemaru J, Tsutsumi N

      Vol. 115 ( 37 ) page： E8783 - E8792   2018.9

    　More details

  25. Effective usage of sorghum bagasse: Optimization of organosolv pretreatment using 25% 1-butanol and subsequent nanofiltration membrane separation Reviewed

    Teramura H, Sasaki K, Oshima T, Kawaguchi H, Ogino C, Sazuka T, Kondo A

      Vol. 252   page： 157 - 164   2018.3

    　More details

  26. Sucrose purification and repeated ethanol production from sugars remaining in sweet sorghum juice subjected to a membrane separation process Reviewed

    Sasaki K, Tsuge Y, Kawaguchi H, Yasukawa M, Sasaki D, Sazuka T, Kamio E, Ogino C, Matsuyama H, Kondo A

      Vol. 101 ( 15 ) page： 6007 - 6014   2017.8

    　More details

  27. Heap: a highly sensitive and accurate SNP detection tool for low-coverage high-throughput sequencing data Reviewed

    Masaaki Kobayashi, Hajime Ohyanagi, Hideki Takanashi, Satomi Asano, Toru Kudo, Hiromi Kajiya-Kanegae, Atsushi J. Nagano, Hitoshi Tainaka, Tsuyoshi Tokunaga, Takashi Sazuka, Hiroyoshi Iwata, Nobuhiro Tsutsumi, Kentaro Yano

    DNA Research   Vol. 24 ( 4 ) page： 397 - 405   2017.8

    　More details

    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Oxford University Press  

    Recent availability of large-scale genomic resources enables us to conduct so called genome-wide association studies (GWAS) and genomic prediction (GP) studies, particularly with next-generation sequencing (NGS) data. The effectiveness of GWAS and GP depends on not only their mathematical models, but the quality and quantity of variants employed in the analysis. In NGS single nucleotide polymorphism (SNP) calling, conventional tools ideally require more reads for higher SNP sensitivity and accuracy. In this study, we aimed to develop a tool, Heap, that enables robustly sensitive and accurate calling of SNPs, particularly with a low coverage NGS data, which must be aligned to the reference genome sequences in advance. To reduce false positive SNPs, Heap determines genotypes and calls SNPs at each site except for sites at the both ends of reads or containing a minor allele supported by only one read. Performance comparison with existing tools showed that Heap achieved the highest F-scores with low coverage (7X) restriction-site associated DNA sequencing reads of sorghum and rice individuals. This will facilitate cost-effective GWAS and GP studies in this NGS era. Code and documentation of Heap are freely available from https://github.com/meiji-bioinf/heap (29 March 2017, date last accessed) and our web site (http://bioinf.mind.meiji.ac.jp/lab/en/tools.html (29 March 2017, date last accessed)).

    DOI： 10.1093/dnares/dsx012

    Web of Science

    Scopus

    PubMed

  28. Screening of rice mutants with improved saccharification efficiency results in the identification of CONSTITUTIVE PHOTOMORPHOGENIC 1 and GOLD HULL AND INTERNODE 1 Reviewed

    Hirano K, Masuda R, Takase W, Morinaka Y, Kawamura M, Takeuchi Y, Takagi H, Yaegashi H, Natsume S, Terauchi R, Kotake T, Matsushita Y, Sazuka T

      Vol. 246 ( 1 ) page： 61 - 74   2017.7

    　More details

    Authorship：Last author,　Corresponding author  

    DOI： 10.1007/s00425-017-2685-9

    Web of Science

  29. Differences in glucose yield of residues from among varieties of rice, wheat, and sorghum after dilute acid pretreatment. Reviewed

    Teramura, H., Sasaki, K., Kawaguchi, H., Matsuda, F., Kikuchi, J., Shirai, T., Sazuka, T., Yamasaki, M., Takumi S., Ogino, C., and Kondo, A.

    Biosci Biotech Biochem.     2017.6

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

  30. Differences in glucose yield of residues from among varieties of rice, wheat, and sorghum after dilute acid pretreatment Reviewed

    Teramura H, Sasaki K, Kawaguchi H, Matsuda F, Kikuchi J, Shirai T, Sazuka T, Yamasaki M, Takumi S, Ogino C, Kondo A

    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY   Vol. 81 ( 8 ) page： 1650 - 1656   2017

    　More details

  31. Growth Properties and Biomass Production in the Hybrid C4 Crop Sorghum bicolor. Reviewed

    Tazoe Y, Sazuka T, Yamaguchi M, Saito C, Ikeuchi M, Kanno K, Kojima S, Hirano K, Kitano H, Kasuga S, Endo T, Fukuda H, Makino A.

    Plant Cell Physiol.     2016.5

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1093/pcp/pcv158.

  32. Organosolv pretreatment of sorghum bagasse using a low concentration of hydrophobic solvents such as 1-butanol or 1-pentanol.

    J Biosci Bioeng.   Vol. 9   page： 27   2016.2

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

  33. Nanofiltration concentration of extracellular glutathione produced by engineered Saccharomyces cerevisiae.

    Sasaki K, Hara KY, Kawaguchi H, Sazuka T, Ogino C, Kondo A.

    J Biosci Bioeng.     2016.1

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.jbiosc.2015.05.013.

  34. O-18 Simultaneous saccharification and fermentation of lignocellulosic biomass for phenyllactate production by recombinant Escherichia coli Reviewed

    川口,秀夫, 寺村,浩, 中村,聡子, 荻野,千秋, 原,清敬, 蓮沼,誠久, 老沼,研一, 高谷,直樹, 平野,恒, 佐塚,隆志, 北野,英己, 近藤,昭彦

    Proceedings of the Conference on Biomass Science   Vol. 11   page： 35 - 36   2016.1

    　More details

    Language：Japanese   Publishing type：Research paper (international conference proceedings)   Publisher：The Japan Institute of Energy  

    Sorghum bagasse pretreated with diluted acid, which was predominantly composed of glucan (59%) and xylan (7.2%), was used as a lignocellulosic feedstock to produce D-phenyllactic acid (PhLA) by a recombinant Escherichia coli strain expressing phenylpyruvate reductase from Wickerhamia fluorescens. Compared to filter paper hydrolysate, the PhLA yield was reduced by 35% during fermentation with enzymatic hydrolysate of sorghum bagasse as a carbon source, and metabolomics analysis revealed that intracellular levels of erythrose-4-phosphate and phosphoenolpyruvate and NAD(P)H regeneration for PhLA production from glucose markedly reduced. Compared to the separate hydrolysis and fermentation (SHF) with sorghum bagasse hydrolysate, simultaneous saccharification and fermentation (SSF) of sorghum bagasse under glucose limitation conditions yielded 4.8-fold more PhLA with less accumulation of eluted components of p-coumaric acid and aldehydes, which inhibited PhLA fermentation. These results suggest that gradual hydrolysis of sorghum bagasse during SSF reduces the accumulation of both glucose and fermentation inhibitors, collectively leading to increased PhLA yield.

    DOI： 10.20550/jiebiomassronbun.11.0_35

    CiNii Books

  35. 3-Amino-4-hydroxybenzoic acid production from sweet sorghum juice by recombinant Corynebacterium glutamicum Reviewed International journal

    Hideo Kawaguchi, Kengo Sasaki, Kouji Uematsu, Yota Tsuge, Hiroshi Teramura, Naoko Okai, Sachiko Nakamura-Tsuruta, Yohei Katsuyama, Yoshinori Sugai, Yasuo Ohnishi, Ko Hirano, Takashi Sazuka, Chiaki Ogino, Akihiko Kondo

    Bioresource Technology   Vol. 198   page： 410 - 417   2015.12

    　More details

    Language：English   Publishing type：Research paper (scientific journal)   Publisher：ELSEVIER SCI LTD  

    The production of the bioplastic precursor 3-amino-4-hydroxybenzoic acid (3,4-AHBA) from sweet sorghum juice, which contains amino acids and the fermentable sugars sucrose, glucose and fructose, was assessed to address the limitations of producing bio-based chemicals from renewable feedstocks. Recombinant Corynebacterium glutamicum strain KT01 expressing griH and griI derived from Streptomyces griseus produced 3,4-AHBA from the sweet sorghum juice of cultivar SIL-05 at a final concentration (1.0 g l(-1)) that was 5-fold higher than that from pure sucrose. Fractionation of sweet sorghum juice by nanofiltration (NF) membrane separation (molecular weight cut-off 150) revealed that the NF-concentrated fraction, which contained the highest concentrations of amino acids, increased 3,4-AHBA production, whereas the NF-filtrated fraction inhibited 3,4-AHBA biosynthesis. Amino acid supplementation experiments revealed that leucine specifically enhanced 3,4-AHBA production by strain KT01. Taken together, these results suggest that sweet sorghum juice is a potentially suitable feedstock for 3,4-AHBA production by recombinant C. glutamicum. (C) 2015 Elsevier Ltd. All rights reserved.

    DOI： 10.1016/j.biortech.2015.09.024

    Web of Science

    Scopus

    PubMed

    J-GLOBAL

  36. Nanofiltration concentration of extracellular glutathione produced by engineered Saccharomyces cerevisiae.

    Sasaki K, Hara KY, Kawaguchi H, Sazuka T, Ogino C, Kondo A.

    J Biosci Bioeng.     2015.6

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.jbiosc.2015.05.013.

  37. Repeated ethanol production from sweet sorghum juice concentrated by membrane separation. Bioresour Technol.

    Sasaki K, Tsuge Y, Sasaki D, Kawaguchi H, Sazuka T, Ogino C, Kondo A.

    Bioresour Technol.     2015.6

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.biortech.2015.03.127.

  38. Phenyllactic acid production by simultaneous saccharification and fermentation of pretreated sorghum bagasse.

    Kawaguchi H, Teramura H, Uematsu K, Hara KY, Hasunuma T, Hirano K, Sazuka T, Kitano H, Tsuge Y, Kahar P, Niimi-Nakamura S, Oinuma K, Takaya N, Kasuga S, Ogino C, Kondo A.

    Bioresour Technol.     2015.4

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.biortech.2015.01.097.

  39. Repeated ethanol production from sweet sorghum juice concentrated by membrane separation.

    Sasaki K, Tsuge Y, Sasaki D, Kawaguchi H, Sazuka T, Ogino C, Kondo A.

    Bioresour Technol.   Vol. 186   page： 351-355   2015.1

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.biortech.2015.03.127.

  40. Increased ethanol production from sweet sorghum juice concentrated by a membrane separation process.

    Sasaki K, Tsuge Y, Sasaki D, Teramura H, Wakai S, Kawaguchi H, Sazuka T, Ogino C, Kondo A.

    Bioresour Technol.     2014.10

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.biortech.2014.07.082.

  41. Phenyllactic acid production by simultaneous saccharification and fermentation of pretreated sorghum bagasse.

    Kawaguchi H, Teramura H, Uematsu K, Hara KY, Hasunuma T, Hirano K, Sazuka T, Kitano H, Tsuge Y, Kahar P, Niimi-Nakamura S, Oinuma K, Takaya N, Kasuga S, Ogino C, Kondo A.

    Bioresour Technol.     2014.4

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.biortech.2015.01.097.

  42. From Genome to Breeding: Challenge of Genome-Assisted Breeding of Sorghum.

    Sazuka T and Iwata H.

    J. Jpn Inst. Enregy   Vol. 93   page： 429-435   2014

    　More details

    Language：English  

  43. Thioredoxin h regulates calcium dependent protein kinases in plasma membranes.

    Ueoka-Nakanishi, H., Sazuka, T., Nakanishi, Y., Maeshima, M., Mori, H. and Hisabori, T.

    FEBS J.   Vol. 280   page： 3220-3231   2013

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

  44. A high-throughput phenotyping system supporting genomic breeding of crop plants International journal

    Iwata H., Kobayashi M., Ohyanagi H., Yano K., Sazuka T., Tokunaga T., Tsutsumi N.

    Proceedings of the SICE Annual Conference     page： 1778 - 1779   2013

    　More details

    Language：English   Publishing type：Research paper (international conference proceedings)   Publisher：Proceedings of the SICE Annual Conference  

    High-throughput technologies for determining DNA sequence variations have enabled new genomics-based breeding strategies, such as genomic selection. While genomics technologies are making rapid progress in throughput and cost efficiencies, technologies for measuring plant phenotypes, i.e., "phenotyping", remain inefficient. Phenotyping is now the most time-consuming and error-prone step in genomic-based breeding strategies. To measure plant phenotypes with high efficiency and accuracy, we are developing a high-throughput phenotyping system. The system consists of two main parts: The plant management system using radio-frequency identification device (RFID) tags and the data collection system connected to multiple measuring devices via Bluetooth. The first system enables us to link plants to data on the Internet and to keep records of cultivation management. The second system enables us to collect data measured by multiple devices via Bluetooth connections. With this system, we can aggregate various types of measurement data in an efficient and precise manner. High-throughput phenotyping systems lead to efficient and accurate data collection and will strongly support genomic breeding in crop plants.

    Scopus

  45. Mapping and characterization of the rice gene, CLUB-SHAPED EMBRYO3, CLE3. Reviewed

    Imamura K, Nagato Y, Matsuoka M, Kitano H, Sazuka T.

    Rice Gen. Newslett.   Vol. 25 ( 1 ) page： 27-29   2010.5

    　More details

    Authorship：Last author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

  46. A rice tryptophan deficient dwarf mutant, tdd1, contains a reduced level of indole acetic acid and develops abnormal flowers and organless embryos. Reviewed

    Sazuka T, Kamiya N, Nishimura T, Ohmae K, Sato Y, Imamura K, Nagato Y, Koshiba T, Nagamura Y, Ashikari M, Kitano H, Matsuoka M.

    Plant J.   Vol. 60 ( 2 ) page： 227-241   2009.10

    　More details

    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

  47. Mapping and Analysis of the CLUB-SHAPED EMBRYO1, CLE1. Reviewed

      Vol. 24 ( 1 ) page： 22-24   2008.9

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

  48. Possible thioredoxin targets of signaling molecules in plasma membranes Reviewed International journal

    Ueoka-Nakanishi Hanayo, Sazuka Takashi, Maeshima Masayoshi, Hisabori Toru

    PLANT AND CELL PHYSIOLOGY   Vol. 48   page： S41 - S41   2007

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    Web of Science

  49. Analysis of club-shaped embryol, a loss of function mutant of rice DICER-LIKE1

    Kohei Imamura, Hanako Imai, Makoto Matsuoka, Hidemi Kitano, Takashi Sazuka

    PLANT AND CELL PHYSIOLOGY   Vol. 48   page： S36 - S36   2007

    　More details

    Language：English   Publisher：OXFORD UNIV PRESS  

    Web of Science

  50. The rice SPINDLY gene functions as a negative regulator of gibberellin signaling by controlling the suppressive function of the DELLA protein, SLR1, and modulating brassinosteroid synthesis. Reviewed

    Shimada A, Ueguchi-Tanaka M, Sakamoto T, Fujioka S, Takatsuto S, Yoshida S, Sazuka T, Ashikari, M, Matsuoka M.

    Plant J.   Vol. 48 ( 3 ) page： 390-402   2006.11

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

  51. Exploring the thioredoxin targets in plant membrane proteins

    H Ueoka-Nakanishi, M Maeshima, T Sazuka, T Hisabori

    PLANT AND CELL PHYSIOLOGY   Vol. 47   page： S78 - S78   2006

    　More details

    Language：English   Publisher：OXFORD UNIV PRESS  

    Web of Science

  52. The rice mutant dwarf bamboo shoot 1: A leaky mutant of the NACK-type kinesin-like gene can initiate organ primordia but not organ development Reviewed International journal

    Sazuka T, Aichi I, Kawai T, Matsuo N, Kitano H, Matsuoka M

    PLANT AND CELL PHYSIOLOGY   Vol. 46 ( 12 ) page： 1934 - 1943   2005.12

    　More details

    Authorship：Lead author   Language：English  

    DOI： 10.1093/pcp/pci206

    Web of Science

  53. Analysis of the rice mutant dwarf and gladius leaf 1. Aberrant katanin-mediated microtubule organization causes up-regulation of gibberellin biosynthetic genes independently of gibberellin signaling Reviewed International journal

    Komorisono M, Ueguchi-Tanaka M, Aichi I, Hasegawa Y, Ashikari M, Kitano H, Matsuoka M, Sazuka T

    PLANT PHYSIOLOGY   Vol. 138 ( 4 ) page： 1982 - 1993   2005.8

    　More details

    Authorship：Corresponding author   Language：English  

    DOI： 10.1104/pp.105.062968

    Web of Science

  54. Isolation of intact vacuoles and proteomic analysis of tonoplast from suspension-cultured cells of Arabidopsis thaliana. Reviewed

    Shimaoka T, Ohnishi M, Sazuka T, Mitsuhashi N, Hara-Nishimura I, Shimazaki KI, Maeshima M, Yokota A, Tomizawa KI, Mimura T.

    Plant Cell Physiol.   Vol. 45 ( 6 ) page： 672-683   2004.6

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

  55. A proteomic approach to identification of transmembrane proteins and membrane-anchored proteins of Arabidopsis thaliana by peptide Sequencing Reviewed International journal

    Sazuka T, Keta S, Shiratake K, Yamaki S, Shibata D

    DNA RESEARCH   Vol. 11 ( 2 ) page： 101 - 113   2004.4

    　More details

    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

    Web of Science

  56. SigC, the group 2 sigma factor of RNA polymerase, contributes to the late-stage gene expression and nitrogen promoter recognition in the Cyanobacterium Synechocystis sp. strain PCC 6803. Reviewed

      Vol. 68 ( 3 ) page： 477-487   2004.3

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

  57. Analysis of dwarf and abnormal leaf morphology 1 (dal1) in rice Invited Reviewed International journal

    T Sazuka, M Komorisono, M Ueguchi-Tanaka, H Kitano, M Ashikari, M Matsuoka

    PLANT AND CELL PHYSIOLOGY   Vol. 45   page： S113 - S113   2004

    　More details

    Language：English   Publishing type：Research paper (scientific journal)   Publisher：OXFORD UNIV PRESS  

    Web of Science

  58. The proteome analysis of the Cyanobacterium Anabaena sp. strain PCC 7120 and classification of the peptides detectable by MALDI-TOF/MS.

    Ojima N, Sazuka T, Yamaguchi M, Kuriki T, Akinaga N, Ando E.

    51st ASMS Conference on Mass Spectrometry and Allied Topics     page： 507   2003

    　More details

    Language：English  

  59. A proteomic approach to identification of transmembrane proteins and membrane-anchored proteins of Arabidopsis thaliana Reviewed International coauthorship International journal

    T Sazuka, S Keta, K Shiratake, S Yamaki, D Shibata

    PLANT AND CELL PHYSIOLOGY   Vol. 44   page： S18 - S18   2003

    　More details

    Language：English   Publishing type：Research paper (scientific journal)   Publisher：OXFORD UNIV PRESS  

    Web of Science

  60. Proteomic analysis of the cyanobacterium Anabaena sp strain PCC7120 with two-dimensional gel electrophoresis and amino-terminal sequencing Reviewed International journal

    Sazuka T

    PHOTOSYNTHESIS RESEARCH   Vol. 78 ( 3 ) page： 279 - 291   2003

    　More details

    Authorship：Lead author,　Last author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

    Web of Science

  61. Isolation and characterization of intact vacuoles from suspension-cultured Arabidopsis cells

    M Ohnishi, N Mitsuhashi, Y Sekiguchi, T Sazuka, K Shimazaki, Nishimura, I

    PLANT AND CELL PHYSIOLOGY   Vol. 44   page： S85 - S85   2003

    　More details

    Language：English   Publisher：OXFORD UNIV PRESS  

    Web of Science

  62. Proteomics of Arabidopsis thaliana: a comprehensive analysis of the strongly hydrophobic membrane proteins Reviewed

    T Sazuka, S Keta, K Shiratake, S Yamaki, D Shibata

    PLANT AND CELL PHYSIOLOGY   Vol. 43   page： S207 - S207   2002

    　More details

    Language：English   Publishing type：Research paper (scientific journal)   Publisher：OXFORD UNIV PRESS  

    Web of Science

  63. Prediction of the coding sequences of unidentified human genes. II. The coding sequences of 40 new genes (KIAA0041-KIAA0080) deduced by analysis of cDNA clones from human cell line KG-1 (supplement). Reviewed

    N. Nomura, T. Nagase, N. Miyajima, T. Sazuka, A. Tanaka, S. Sato, N. Seki, Y. Kawarabayasi, K. Ishikawa, S. Tabata

    DNA research   Vol. 1   page： 251 - 262   1994.12

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1093/dnares/1.5.251

    Scopus

    PubMed

  64. Prediction of the coding sequences of unidentified human genes. I. The coding sequences of 40 new genes (KIAA0001-KIAA0040) deduced by analysis of randomly sampled cDNA clones from human immature myeloid cell line KG-1 (supplement).

    N. Nomura, N. Miyajima, T. Sazuka, A. Tanaka, Y. Kawarabayasi, S. Sato, T. Nagase, N. Seki, K. Ishikawa, S. Tabata

    DNA research : an international journal for rapid publication of reports on genes and genomes   Vol. 1 ( 1 ) page： 47 - 56   1994

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1093/dnares/1.1.47

    Scopus

    PubMed

  65. Identification of a developmentally regulated gene in the mouse central nervous system which encodes a novel proline rich protein Reviewed

    Takashi Sazuka, Yasuhiro Tomooka, Sandeep Kathju, Yoji Ikawa, Makoto Noda, Sharad Kumar

    BBA - Gene Structure and Expression   Vol. 1132 ( 3 ) page： 240 - 248   1992.10

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    A full length cDNA whose corresponding mRNA is down-regulated during the mouse embryonic brain development was isolated. The cDNA contains a single long open reading frame which could encode a protein with relative molecular mass of 41 kDa. The predicted gene product contains long stretches of prolines towards the NH2-terminus, followed by a leucine/proline rich region. The cDNA probe detected a number of mRNA species in Northern blot analysis. The reverse transcriptase-polymerase chain reaction analysis of mRNA from adult mouse tissues indicated that heart and testis expressed this gene (named NDPP-1) at relatively high levels, while lower levels of mRNA were detected in a number of other tissues. Expression of NDPP-1 was also detected in embryonic carcinoma and pheochromocytoma cell lines, but not in fibroblasts. The cDNA hybridized to genomic DNA from several vertebrates species in Southern blot analysis indicating interspecies conservation of this gene. The interesting pattern of expression of the NDPP-1 gene during mouse brain development and the structure of its putative protein product indicate that this gene may play an important biological role in the development of mouse central nervous system. © 1992.

    DOI： 10.1016/0167-4781(92)90156-T

    Scopus

    PubMed

▼display all

To the head of Papers.▲

Books 23

  1. Photo-switched Biodegradation of Bioplastics in Marine Environments Reviewed International journal

    Hideo Kawaguchi, Takashi Sazuka, Dao Duy Hanh（ Role： Joint author ,  Biorefinery: Microbial Production of Building Blocks from Plant Resources for the Synthesis of Bioplastics）

    Springer  2023.9  （ ISBN:9789819943531 ）

    　More details

    Total pages：21   Responsible for pages：p19-p30   Language：English Book type：Scholarly book

    DOI： 10.1007/978-981-99-4354-8_2

  2. 草本バイオマスの増産と利用

    （ Role： Supervisor (editorial)）

    北隆館  2023.6 

    　More details

    Language：Japanese Book type：Scholarly book

  3. 作物育種で持続可能社会を創り出す

    佐塚隆志（ Role： Sole author）

    養賢堂  2019 

    　More details

    Total pages：14-18   Responsible for pages：14-18   Language：Japanese Book type：Scholarly book

  4. ソルガムの茎糖液含量を左右する乾汁性決定遺伝子 D

    藤本優、佐塚隆志、小田祥久、春日重光、米丸淳一、堤伸浩（ Role： Joint author）

    シーエムシー出版  2018 

    　More details

    Total pages：112-115   Language：Japanese Book type：Scholarly book

  5. Molecular Breeding of Sorghum bicolor, A Novel Energy Crop.

    Ordonio R, Ito Y, Morinaka Y, Sazuka T, and Matsuoka M.（ Role： Joint author）

    Elsevier Inc.  2016 

    　More details

    Language：Japanese

  6. 高バイオマス性と高糖性を併せ持つバイオリファイナリー用ソルガムの育種開発.「バイオインダストリー」

    佐塚隆志（ Role： Sole author）

    シーエムシー出版  2014 

    　More details

    Language：Japanese

  7. ラン藻. 「ゲノミクス・プロテオミクスの新展開―生物情報の解析と応用―」

    （ Role： Sole author）

    エヌ・ティー・エス  2004 

    　More details

    Language：Japanese

  8. シロイヌナズナ. 「ゲノミクス・プロテオミクスの新展開―生物情報の解析と応用―」

    （ Role： Sole author）

    エヌ・ティー・エス  2004 

    　More details

    Language：Japanese

  9. シロイヌナズナ. 「ゲノミクス・プロテオミクスの新展開―生物情報の解析と応用―」

    エヌ・ティー・エス  2004 

  10. ラン藻. 「ゲノミクス・プロテオミクスの新展開―生物情報の解析と応用―」

    エヌ・ティー・エス  2004 

  11. シロイヌナズナ・ゲノムから見た膜輸送系.「植物細胞工学シリーズ別冊18・植物細胞で働くポンプ・トランスポーター・チャンネル研究の新展開」

    （ Role： Joint author）

    秀潤社  2003 

    　More details

    Language：Japanese

  12. シロイヌナズナ・ゲノムから見た膜輸送系.「植物細胞工学シリーズ別冊18・植物細胞で働くポンプ・トランスポーター・チャンネル研究の新展開」

    秀潤社  2003 

  13. MS-FIT.「バイオデータベースの手取り足取り活用法」

    羊土社  2002 

    　More details

    Language：Japanese

  14. 植物ゲノム解読とオルガネラのプロテオミクス. 「植物細胞工学シリーズ別冊17・植物オルガネラの分化と多様性」

    秀潤社  2002 

  15. ラン藻及び高等植物のプロテオーム解析.「プロテオミクスの最新技術」

    シーエムシー出版  2002 

    　More details

    Language：Japanese

  16. プロテオーム時代に生きる. Peptide News Letter

    日本ペプチド学会  2001 

    　More details

    Language：Japanese

  17. ラン藻及び高等植物のプロテオーム解析. 「バイオインダストリー」

    （ Role： Sole author）

    シーエムシー出版  2001 

    　More details

    Language：Japanese

  18. ラン藻及び高等植物のプロテオーム解析. 「バイオインダストリー」

    シーエムシー出版  2001 

  19. シアノバクテリアのプロテオーム解析

    細胞工学  2000 

    　More details

    Language：Japanese

  20. ラン藻のプロテオーム解析. 「実験医学別冊・ポストゲノム時代の実験講座2・プロテオーム解析法」

    羊土社  2000 

  21. Genes with potential for suppressing malignant phenotypes of Kirsten sarcoma virus-transformed NIH/3T3 cells. In Moses H.L., Lengyel, P. and Stiles, C.D., eds., Growth Inhibitory and Cytotoxic Polypeptides

    （ Role： Joint author）

    Alan R. Liss  1989 

    　More details

    Language：English

  22. Genes with potential for suppressing malignant phenotypes of Kirsten sarcoma virus-transformed NIH/3T3 cells. In Moses H.L., Lengyel, P. and Stiles, C.D., eds., Growth Inhibitory and Cytotoxic Polypeptides International journal

    （ Role： Joint author）

    Alan R. Liss  1989 

    　More details

    Language：English Book type：Scholarly book

  23. Genes with potential for suppressing malignant phenotypes of Kirsten sarcoma virus-transformed NIH/3T3 cells. In Moses H.L., Lengyel, P. and Stiles, C.D., eds., Growth Inhibitory and Cytotoxic Polypeptides

    Alan R. Liss  1989 

▼display all

To the head of Books.▲

MISC 53

  1. 脱炭素社会構築に貢献する草本バイオマスの可能性 Invited

    佐塚隆志

    アグリバイオ   Vol. 7 ( 6 ) page： 6 - 7   2023.6

    　More details

    Authorship：Lead author,　Last author,　Corresponding author   Language：Japanese   Publishing type：Article, review, commentary, editorial, etc. (scientific journal)  

  2. セルロース系バイオマスを原料とするカフェ酸発酵のためのゲノム編集による大腸菌代謝改変の作製と培養法の検討

    川口秀夫, 宮崎翔子, 寺本潤, 西田敬二, 南博道, 佐塚隆志, 近藤昭彦, 近藤昭彦

    日本生物工学会大会講演要旨集   Vol. 71st   2019

    　More details

  3. 育種計画を効率化する圃場情報管理システムの構築と活用

    小林正明, 浅野さとみ, 釜付香, 釜付香, 大柳一, 大柳一, 鐘ケ江弘美, 高梨秀樹, 徳永毅, 佐塚隆志, 岩田洋佳, 堤伸浩, 矢野健太郎

    育種学研究   Vol. 19   2017

    　More details

  4. 疎水性アルコールを用いた植物バイオマスの効率的な成分分離法の開発

    寺村浩, 佐々木建吾, 白井智量, 川口秀夫, 荻野千秋, 菊地淳, 佐塚隆志, 近藤昭彦, 近藤昭彦

    リグニン討論会講演集   Vol. 61st   page： 180‐181   2016.10

    　More details

    Language：Japanese   Publishing type：Research paper, summary (national, other academic conference)  

    J-GLOBAL

  5. アルコールの添加が希硫酸前処理の効率に与える影響の解析

    寺村浩, 佐々木建吾, 白井智量, 川口秀夫, 荻野千秋, 菊地淳, 佐塚隆志, 近藤昭彦

    日本農芸化学会大会講演要旨集(Web)   Vol. 2016   page： 3A030 (WEB ONLY)   2016.3

    　More details

    Language：Japanese  

    J-GLOBAL

  6. ソルガムのHapMapの構築とゲノム育種への利用

    KANEGAE HIROMI, MOCHIZUKI TAKAKO, KAMINUMA ERI, MINAMIKAWA MAI, KOBAYASHI MASAAKI, TOYOSHIMA HIROMI, OYANAGI HAJIME, TAKANASHI HIDEKI, NAGANO ATSUSHI, TOKUNAGA TAKESHI, SAZUKA TAKASHI, YANO KENTARO, NAKAMURA YASUKAZU, TSUTSUMI NOBUHIRO, IWATA HIROYOSHI

    育種学研究   Vol. 16   page： 32   2014.9

    　More details

    Language：Japanese  

    DOI： 10.1270/jsbbr.16.32

    J-GLOBAL

  7. From Genome to Breeding : Challenge of Genome-Assisted Breeding of Sorghum

    SAZUKA Takashi, IWATA Hiroyoshi

    Journal of the Japan Institute of Energy   Vol. 93 ( 5 ) page： 429 - 435   2014.5

    　More details

    Language：Japanese   Publisher：The Japan Institute of Energy  

    Sorghum (Sorghum bicolor (L.) Moench) is a high-biomass C4 crop with high photosynthetic efficiency. It can tolerate severe climatic conditions such as drought, soil salinity or high temperatures. Many cultivars of sorghum have been bred for specific purposes, for example, "biomass sorghum" (many cultivars are F_1 hybrids) with extremely high biomass were bred for high silage yield, and "sweet sorghums" (many cultivars are inbreds) with high sugar content in the culm (stalk) juice were bred for syrup production. This clear delineation of the two means that there is currently no cultivar possessing both extremely high biomass and high sugar content, which is actually the type highly suitable for our "biorefinery model" involving a cascade of sugar extraction and bagasse production from a large amount of sorghum biomass. Thus, we are taking the challenge of producing a new cultivar that possesses the two traits important for biorefinery use through DNA Marker-Assisted Selection (MAS). In this review, we introduced some basic studies to target our breeding goal, QTL analyses for sweetness and culm height. Another new challenge, the application of Genomic Selection (GS) to sorghum breeding is also discussed.

    CiNii Books

  8. 3P-045 Effects of hydrolysate of lignocellulosic biomass on phenyllactate fermentation by recombinant Escherichia coli

    Kawaguchi Hideo, Teramura Hiroshi, Niimi-Nakamura Satoko, Ogino Chiaki, Hara Kiyotaka, Hasunuma Tomohisa, Oinuma Ken-Ichi, Takaya Naoki, Hirano Ko, Sazuka Takashi, Kitano Hidemi, Kondo Akihiko

      Vol. 66   page： 206 - 206   2014

    　More details

    Language：Japanese   Publishing type：Meeting report  

    CiNii Books

    Other Link： http://search.jamas.or.jp/link/ui/2016046044

  9. バイオエネルギー作物・ソルガムの高速ジェノタイピングにおけるバイオインフォマティクス手法の検討・開発

    OYANAGI HAJIME, KOBAYASHI MASAAKI, TOYOSHIMA HIROMI, TAKANO TOMOYUKI, TAKANASHI HIDEKI, NAGANO ATSUSHI, TAINAKA HITOSHI, TOKUNAGA TAKESHI, SAZUKA TAKASHI, IWATA HIROYOSHI, TSUTSUMI NOBUHIRO, YANO KENTARO

    育種学研究   Vol. 15   page： 110   2013

    　More details

    Language：Japanese  

    DOI： 10.1270/jsbbr.15.110

    J-GLOBAL

  10. Yamamoto, Y., Inukai, Y., Kitano, H., Sazuka, T. and Matsuoka, M. Characterization and mapping of the CROWN ROOTLESS2 gene, CRL2, in rice. Reviewed International journal

    Rice Gen. Newslet.   Vol. 25 ( 1 ) page： 25 - 26   2010

    　More details

    Authorship：Last author,　Corresponding author   Language：English   Publishing type：Rapid communication, short report, research note, etc. (scientific journal)  

  11. Yamamoto, Y., Inukai, Y., Kitano, H., Sazuka, T. and Matsuoka, M. Characterization and mapping of the CROWN ROOTLESS2 gene, CRL2, in rice.

    Rice Gen. Newslet.   Vol. 25 ( 1 ) page： 25 - 26   2010

  12. Mapping and characterization of the rice gene, CLUB-SHAPED EMBRYO3, CLE3.

    Rice Gen. Newslet.   Vol. 25 ( 1 ) page： 27 - 29   2010

  13. Mapping and characterization of the rice gene, CLUB-SHAPED EMBRYO3, CLE3.

    Rice Gen. Newslet.   Vol. 25 ( 1 ) page： 27 - 29   2010

  14. Mapping and Analysis of the CLUB-SHAPED EMBRYO1, CLE1.

    Rice Gen. Newslet.   Vol. 24 ( 1 ) page： 22 - 24   2008

  15. Mapping and Analysis of the CLUB-SHAPED EMBRYO1, CLE1.

      Vol. 24 ( 1 ) page： 22 - 24   2008

  16. Analysis of club-shaped embryol, a loss of function mutant of rice DICER-LIKE1 Invited International journal

    Imamura Kohei, Imai Hanako, Matsuoka Makoto, Kitano Hidemi, Sazuka Takashi

    PLANT AND CELL PHYSIOLOGY   Vol. 48   page： S36 - S36   2007

    　More details

    Authorship：Last author,　Corresponding author   Publishing type：Meeting report  

    Web of Science

  17. Exploring the thioredoxin targets in plant membrane proteins International journal

    H Ueoka-Nakanishi, M Maeshima, T Sazuka, T Hisabori

    PLANT AND CELL PHYSIOLOGY   Vol. 47   page： S78 - S78   2006

    　More details

    Language：English   Publishing type：Research paper, summary (international conference)   Publisher：OXFORD UNIV PRESS  

    Web of Science

  18. SigC, the group 2 sigma factor of RNA polymerase, contributes to the late-stage gene expression and nitrogen promoter recognition in the cyanobacterium Synechocystis sp strain PCC 6803

    M Asayama, S Imamura, S Yoshihara, A Miyazaki, N Yoshida, T Sazuka, T Kaneko, O Ohara, S Tabata, T Osanai, K Tanaka, H Takahashi, M Shirai

    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY   Vol. 68 ( 3 ) page： 477 - 487   2004.3

    　More details

    Language：English   Publisher：TAYLOR & FRANCIS LTD  

    We examined the role of SigC (Sll0184), a sigma factor of RNA polymerase (RNAP), in a unicellular cyanobacterium, Synechocystis sp. strain PCC 6803. On the inactivation of sigC, which is an Escherichia coli rpoD homolog, cells were viable but had a low survival rate in the stationary phase of growth under normal physiological conditions, indicating that SigC is a group 2 type sigma factor. In analyses of transcript and protein levels using the sigC knockout strain, it was found that expression of glnB, a nitrogen key regulatory gene, is controlled by SigC in the stationary phase. Primer extension revealed that the glnB nitrogen promoter (P2) was specifically recognized by SigC in the stationary phase under conditions of nitrogen starvation. In vitro studies with purified enzymes indicated effective transcription, on supercoiled DNA templates, from P2 by SigC-RNAP with NtcA which is an activator for nitrogen gene transcription. DNase I footprinting also indicated binding and related sites of NtcA and/or RNAP with SigC on the nitrogen promoter. The unique promoter architecture and the mechanism of transcription by RNAP with SigC are also discussed.

    DOI： 10.1271/bbb.68.477

    Web of Science

    PubMed

  19. Identification of membrane-bound proteins from a vacuolar membrane-enriched fraction of <i>Arabidopsis thaliana</i> Reviewed

    Sazuka, T, Keta, S, Shiratake, K, Yamaki, S, Shibata, D

    DNA Research   Vol. 11   page： 101-113   2004

    　More details

    Language：English   Publishing type：Rapid communication, short report, research note, etc. (scientific journal)  

  20. Isolation and characterization of intact vacuoles from suspension-cultured Arabidopsis cells International journal

    Ohnishi M, Mitsuhashi N, Sekiguchi Y, Sazuka T, Shimazaki K, Nishimura I

    PLANT AND CELL PHYSIOLOGY   Vol. 44   page： S85 - S85   2003

    　More details

    Language：English   Publishing type：Article, review, commentary, editorial, etc. (international conference proceedings)  

    Web of Science

  21. The proteome analysis of the Cyanobacterium Anabaena sp. strain PCC 7120 and classification of the peptides detectable by MALDI-TOF/MS.

    51st ASMS Conference on Mass Spectrometry and Allied Topics     page： 507   2003

  22. The proteome analysis of the Cyanobacterium Anabaena sp. strain PCC 7120 and classification of the peptides detectable by MALDI-TOF/MS.

    51st ASMS Conference on Mass Spectrometry and Allied Topics     page： 507   2003

  23. Proteome Approach to Proteins Presented at The Vacuolar Membrane from Arabidopsis thaliana

    KETA S., SHIRATAKE K., SAZUKA T., SHIBATA D., MAESHIMA M., YAMAKI S.

      Vol. 71 ( 1 ) page： 371 - 371   2002.4

    　More details

    Language：Japanese  

    CiNii Books

  24. MS-FIT.「バイオデータベースの手取り足取り活用法」

    羊土社   Vol. 第6章   page： 190   2002

  25. 植物ゲノム解読とオルガネラのプロテオミクス. 「植物細胞工学シリーズ別冊17・植物オルガネラの分化と多様性」

    秀潤社   Vol. 第4章-1   page： 211 - 219   2002

  26. ラン藻及び高等植物のプロテオーム解析.「プロテオミクスの最新技術」

    シーエムシー出版   Vol. 第2章   page： 11 - 20   2002

  27. ISOLATION OF VACUOLAR MEMBRANE FROM Arabidopsis AND IDENTIFICATION OF ITS PROTEINS :

    KETA Sumie, SHIRATAKE Katsuhiro, SAZUKA Takashi, SHIBATA Daisuke, MAESHIMA Masayoshi, YAMAKI Shohei

    Plant and cell physiology   Vol. 42   page： s38   2001

    　More details

    Language：English   Publisher：Japanese Society of Plant Physiologists  

    CiNii Books

    Other Link： https://projects.repo.nii.ac.jp/?action=repository_uri&item_id=184610

  28. プロテオーム時代に生きる. Peptide News Letter

    日本ペプチド学会   Vol. 41   page： 7 - 8   2001

  29. 単細胞性ラン藻 Synechocystis sp. strain PCC6803および糸状性ラン藻Anabaena sp. strain PCC7120の比較プロテオーム解析

    生物物理化学   Vol. 45   page： 123 - 128   2001

  30. シアノバクテリアのプロテオーム解析

    細胞工学   Vol. 19   page： 1394 - 1400   2000

  31. ラン藻のプロテオーム解析. 「実験医学別冊・ポストゲノム時代の実験講座2・プロテオーム解析法」

    羊土社   Vol. 第6章I-2   page： 226 - 231   2000

  32. Cyano2Dbase updated: Linkage of 234 protein spots to corresponding genes through N-terminal microsequencing

    T Sazuka, M Yamaguchi, O Ohara

    ELECTROPHORESIS   Vol. 20 ( 11 ) page： 2160 - 2171   1999.8

    　More details

    Language：English   Publisher：WILEY-V C H VERLAG GMBH  

    The cyanobacterium Synechocystis sp. strain PCC6803 is an interesting model organism for preoteome study because it is a photosynthetic procaryote and its genomic sequence has already been determined at our institute. We thus initiated characterization of this organism from a proteomic viewpoint by exploiting two-dimensional (2-D) gel electrophoresis coupled with N-terminal protein sequencing. In a previous study, we linked 130 protein spots on two dimensional gels with the genes that encoded them. As an extension of the previous study, the number of protein spots linked to their corresponding genes was increased to 227 in this study by separately analyzing cyanobacterial proteins in four different fractions (soluble, insoluble, thylakoid membrane, and secretory protein fractions). The resultant updated 2-D protein-gene linkage database, named Cyano2Dbase, will serve as an indispensable tool in future cyanobacterial proteomic studies. From the data compiled in the Cyano2Dbase, we can extract many items of information concerning translation, posttranslational processing including characteristics of cyanobacterial signal sequences and modification of cyanobacterial proteins. The Cyano2Dbase is available to the public through the World Wide Web (http://www.kazusa.or.jp/tech/sazuka/cyano/proteome.html).

    DOI： 10.1002/(SICI)1522-2683(19990801)20:11<2160::AID-ELPS2160>3.0.CO;2-#

    Web of Science

    PubMed

  33. Analysis of brain proteins in Alzheimer's disease using high-resolution two-dimensional gel electrophoresis

    T Tsuji, S Shimohama, S Kamiya, T Sazuka, O Ohara

    JOURNAL OF THE NEUROLOGICAL SCIENCES   Vol. 166 ( 2 ) page： 100 - 106   1999.7

    　More details

    Language：English   Publisher：ELSEVIER SCIENCE BV  

    Two-dimensional gel electrophoresis (2-DE), a method which can be used to analyze the expression of many proteins, is a promising and powerful approach which we have begun to use in the characterization of the complex pathologic processes in Alzheimer's disease (AD). In the present study, a reliable 2-DE database of human brain proteins was created by improving the reproducibility of 2-DE images using an immobilized pH gradient (IPG) for the first dimension gel electrophoresis and Melanie II as the program for data analysis. The brain samples were taken from the temporal cortex of brains at autopsy from 15 AD patients and 15 age-matched controls with non-neurological disorders. About 700 spots were located as consistently expressed proteins in the human brain, all of which were expressed also in AD brains. Comparing the density of spots between AD and normal control, we found that five protein spots were significantly increased, 28 spots were significantly decreased and nine spots were detected only in AD. Two spots among those significantly increased and one spot among those significantly decreased were identified as glial fibrillary acidic proteins. The database of brain proteins in AD constructed for the present study, including the statistical data of density changes in AD, should be a useful beginning for a comprehensive human 2-DE database available via the Internet, which will facilitate further investigation of pathogenic protein alterations in AD. (C) 1999 Elsevier Science B.V. All rights reserved.

    DOI： 10.1016/S0022-510X(99)00120-3

    Web of Science

  34. Analysis of the gene-deficient strain of the cyanobacterial sigma factor

    MIYAZAKI Ai, ASAYAMA Munehiko, SHIRAI Makoto, SAZUKA Takashi, OHARA Osamu

      Vol. 39   page： S125 - S125   1998.5

    　More details

    Language：English  

    CiNii Books

  35. Proteome analysis of cyanobacterium : Synechocystis sp. strain PCC6803

    SAZUKA Takashi, OHARA Osamu

    Seibutsu Butsuri   Vol. 38 ( 5 ) page： 214 - 216   1998

    　More details

    Language：Japanese   Publisher：The Biophysical Society of Japan General Incorporated Association  

    DOI： 10.2142/biophys.38.214

    CiNii Books

    Other Link： https://jlc.jst.go.jp/DN/JALC/00085389830?from=CiNii

  36. Analysis and Prediction of translation initiation sites in photosynthesis genes in Synechocystis by HMM

    HIROSAWA M., YADA T., SAZUKA T.

    Biophysics   Vol. 37   page： S131   1997.10

    　More details

    Language：Japanese   Publisher：The Biophysical Society of Japan General Incorporated Association  

    CiNii Books

  37. Towards a proteome project of cyanobacterium Synechocystis sp. strain PCC6803: Linking 130 protein spots with their respective genes

    T Sazuka, O Ohara

    ELECTROPHORESIS   Vol. 18 ( 8 ) page： 1252 - 1258   1997.8

    　More details

    Language：English   Publisher：VCH PUBLISHERS INC  

    Following the complete sequencing of the genome of the univellular cyanobacterium, Synechocystis sp. strain PCC6803, within our institute, a protein-gene linkage map of this photosynthetic microorganism was successfully constructed for 130 high abundance proteins present on two-dimensional gels. An additional six proteins were analyzed, but were probably encoded extrachromosomally. In order to demonstrate the usefulness of this protein-gene linkage map, we analyzed the changes that occur in cellular proteins after illumination of PCC6803 cells. The results indicate that this protein-gene linkage map greatly simplifies the identification process of such modulated genes. After illumination, at least three distinctive spots with reduced intensity were detected on two-dimensional gels and the corresponding genes of two of these were successfully identified as chaperonin 2 and a Tortula ruralis rehydrin-related gene. Thus, the combination of the protein-gene linkage map and two-dimensional gel electrophoresis should permit a comprehensive analyses of the proteins encoded by the genome (i.e.,''proteome'') of this photosynthetic autotroph. This post-genome project represents a productive way of exploiting the information obtained from the sequencing of the cyanobacterium genome.

    DOI： 10.1002/elps.1150180806

    Web of Science

  38. Analysis of sequence patterns surrounding the translation initiation sites on Cyanobacterium genome using the hidden Markov model

    Tetsushi Yada, Takashi Sazuka, Makoto Hirosawa

    DNA Research   Vol. 4 ( 1 ) page： 1 - 7   1997

    　More details

    Language：English   Publisher：Universal Academy Press Inc.  

    Sequence patterns surrounding the translation initiation sites of Cyanobacterium were precisely analyzed by the hidden Markov model (HMM) based on the actual translation initiation sites. In a previous study, 72 actual protein coding regions and their translation initiation sites on the genome of Synechocystis sp. strain PCC6803 were determined by Sazuka et al. using protein two-dimensional electrophoresis and microsequening. In this work, we extracted the sequence patterns surrounding translation initiation sites as HMM using the computer program YEBIS. The constructed HMM could recognize all but one translation initiation site. The HMM contains an AG-rich region (5.7 bp on average), as the Shine-Dalgarno sequence exclusively contains purines, upstream of the translation initiation site (-9.7 position on average) and a CT-rich region (4.2 bp on average) just upstream from the translation initiation site. In addition, we found that the second amino acid (+4,5,6) could be classified into two types, one of which had C as their second codon while another of which has a nucleotide distribution relatively similar to the distribution among amino acids in the 72 proteins. This fact corresponds well to our earlier finding that when the second nucleotide of the second amino acid of a translated protein was C, an initial methionine was processed and that otherwise the methionine was intact with high frequency.

    DOI： 10.1093/dnares/4.1.1

    Scopus

    PubMed

  39. Prediction of translation initiation sites on the genome of Synechocystis sp. strain PCC6803 by Hidden Markov Model

    Makoto Hirosawa, Takashi Sazuka, Tetsushi Yada

    DNA Research   Vol. 4 ( 3 ) page： 179 - 184   1997

    　More details

    Language：English   Publisher：Universal Academy Press Inc.  

    We developed a computer program, GeneHackerTL, which predicts the most probable translation initiation site for a given nucleotide sequence. The program requires that information be extracted from the nucleotide sequence data surrounding the translation initiation sites according to the framework of the Hidden Markov Model. Since the translation initiation sites of 72 highly abundant proteins have already been assigned on the genome of Synechocystis sp. strain PCC6803 by amino-terminal analysis, we extracted necessary information for GeneHackerTL from the nucleotide sequence data. The prediction rate of the GeneHackerTL for these proteins was estimated to be 86.1%. We then used GeneHackerTL for prediction of the translation initiation sites of 24 other proteins, of which the initiation sites were not assigned experimentally, because of the lack of a potential initiation codon at the amino-terminal position. For 20 out of the 24 proteins, the initiation sites were predicted in the upstream of their amino-terminal positions. According to this assignment, the processed regions represent a typical feature of signal peptides. We could also predict multiple translation initiation sites for a particular gene for which at least two initiation sites were experimentally detected. This program would be effective for the prediction of translation initiation sites of other proteins, not only in this species but also in other prokaryotes as well.

    DOI： 10.1093/dnares/4.3.179

    Scopus

    PubMed

  40. ラン藻(Synechocystis sp. PCC6803)ゲノムの構造解析 VI. 全ゲノム塩基配列とORF解析

    金子 賢一, 小谷 博一, 佐藤 修正, 田中 亜矢子, 浅水 恵理香, 宮嶋 伸行, 広沢 誠, 佐塚 隆志, 杉浦 昌弘, 田畑 哲之

    日本分子生物学会年会プログラム・講演要旨集   Vol. 19   page： 751 - 751   1996.8

    　More details

    Language：Japanese  

    CiNii Books

  41. GENOME SEQUENCING PROJECT OF A CYANOBACTERIUM Synechocystis sp. strain PCC6803

    KANEKO Takakazu, SATO Shusei, KOTANI Hirokazu, TANAKA Ayako, ASAMIZU Erika, MIYAJIMA Nobuyuki, HIROSAWA Makoto, SAZUKA Takashi, SUGIURA Masahiro, TABATA Satoshi

      Vol. 37   page： 51 - 51   1996.3

    　More details

    Language：English  

    CiNii Books

  42. Sequence features surrounding the translation initiation sites assigned on the genome sequence of synechocystis sp. strain PCC6803 by amino-terminal protein sequencing

    Takashi Sazuka, Osamu Ohara

    DNA Research   Vol. 3 ( 4 ) page： 225 - 232   1996

    　More details

    Language：English   Publisher：Universal Academy Press Inc.  

    To characterize the sequence features surrounding the translation initiation sites on the genome of Synechocystis sp. strain 6803, the total proteins extracted from the cell were resolved by two-dimensional electrophoresis, and the amino-terminal sequences of the relatively abundant protein spots were determined. By comparison of the determined amino-terminal sequences with the nucleotide sequence of the entire genome, the translation initiation sites of a total of 72 proteins were successfully assigned on the genome. The sequence features emerged from the nucleotide sequences at and surrounding the translation initiation sites were as follows: (1) In addition to the three initiation codons, ATG, GTG, and TTG, evidence was obtained that ATT was also used as a rare initiation codon
    (2) the core sequences (GAGG, GGAG and AGGA) of the Shine-Dalgarno sequence were identified in the appropriate position preceding the 35 initiation sites (48.6%)
    and (3) the preferential sequence surrounding the initiation codons was formulated as 5′-YY[•••]R-3′ where Y and R denote pyrimidine and purine nucleotides, respectively, and three dots represent the initiation codons. The result obtained would provide valuable information for improvement of the gene-finding software, and the approach used in this study should be applicable for comprehensive analysis of the expression profiles of cellular proteins.

    DOI： 10.1093/dnares/3.4.225

    Scopus

    PubMed

  43. Prediction of the coding sequences of unidentified human genes. III. The coding sequences of 40 new genes (KIAA0081-KIAA0120) deduced by analysis of cDNA clones from human cell line KG-1 (supplement) Reviewed International journal

    DNA Res.   Vol. 2 ( 1 ) page： 51 - 59   1995

    　More details

    Language：English   Publishing type：Rapid communication, short report, research note, etc. (scientific journal)  

  44. Prediction of the coding sequences of unidentified human genes. III. The coding sequences of 40 new genes (KIAA0081-KIAA0120) deduced by analysis of cDNA clones from human cell line KG-1

    Takahiro Nagase, Nobuyuki Miyajima, Ayako Tanaka, Takashi Sazuka, Naohiko Seki, Shusei Sato, Satoshi Tabata, Ken-Ichi Ishikawa, Yutaka Kawarabayasi, Hirokazu Kotani, Nobuo Nomura

    DNA Research   Vol. 2 ( 1 ) page： 37 - 43   1995

    　More details

    Language：English   Publisher：Universal Academy Press Inc.  

    We isolated full-length cDNA clones from size-fractionated cDNA libraries of human immature myeloid cell line KG-1, and the coding sequences of 40 genes were newly predicted. A computer search of the GenBank/EMBL databases indicated that the sequences of 14 genes were unrelated to any reported genes, while the remaining 26 genes carried some sequences with similarities to known genes. Significant transmembrane domains were identified in 17 genes, and protein motifs that matched those in the PROSITE motif database were identified in 11 genes. Northern hybridization analysis with 18 different cells and tissues demonstrated that 10 genes were apparently expressed in a cell-specific or tissue-specific manner. Among the genes predicted, half were isolated from the medium-sized cDNA library and the other half from the small-sized cDNA library, and their average sizes were 4 kb and 1.4 kb, respectively. As judged by Northern hybridization profiles, small-sized cDNAs appeared to be expressed more ubiquitously and abundantly in various tissues, compared with that of medium-sized cDNAs.

    DOI： 10.1093/dnares/2.1.37

    Scopus

    PubMed

  45. Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. I. Sequence features in the 1 Mb region from map positions 64% to 92% of the genome (supplement).

    DNA Res.   Vol. 2 ( 4 ) page： 191 - 198   1995

  46. Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. I. Sequence features in the 1 Mb region from map positions 64% to 92% of the genome (supplement).

    DNA Res.   Vol. 2 ( 4 ) page： 191 - 198   1995

  47. Prediction of the coding sequences of unidentified human genes. III. The coding sequences of 40 new genes (KIAA0081-KIAA0120) deduced by analysis of cDNA clones from human cell line KG-1.

    DNA Res.   Vol. 2 ( 1 ) page： 37 - 43   1995

  48. Prediction of the coding sequences of unidentified human genes. III. The coding sequences of 40 new genes (KIAA0081-KIAA0120) deduced by analysis of cDNA clones from human cell line KG-1 (supplement)

    DNA Res.   Vol. 2 ( 1 ) page： 51 - 59   1995

  49. Prediction of the coding sequences of unidentified human genes. II. The coding sequences of 40 new genes (KIAA0041-KIAA0080) deduced by analysis of cDNA clones from human cell line KG-1

    Nobuo Nomura, Takahiro Nagase, Nobuyuki Miyajima, Takashi Sazuka, Ayako Tanaka, Shusei Sato, Naohiko Seki, Yutaka Kawarabayasi, Ken-Ichi Ishikawa, Satoshi Tabata

    DNA Research   Vol. 1 ( 5 ) page： 223 - 229   1994

    　More details

    Language：English   Publisher：Universal Academy Press Inc.  

    By applying the protocol previously established, we isolated and sequenced full-length cDNA clones longer than 2 kb from a cDNA library of human immature myeloid cell line KG-1, and the coding sequences of 40 new genes were predicted. A computer search of the sequences indicated that 29 genes contained sequences with similarities to reported genes in the GenBank/EMBL databases. Significant transmembrane domains were identified in 9 genes, 5 of which harbored multiple hydrophobic regions. Protein motifs that matched those in the PROSITE motif database were identified in 13 genes. In terms of sequence similarities and protein motifs, 5 genes were related to transcriptional factors. Repetitive sequences were found in the 3′-untranslated region of 8 genes. Northern hybridization demonstrated that the expression of 9 genes was tissue-specific, while the remaining 31 genes were expressed ubiquitously. It was also noted that 17 genes yielded different sizes of bands possibly due to either alternative splicing or alternative initiation. The chromosomal location of these genes has been determined.

    DOI： 10.1093/dnares/1.5.223

    Scopus

    PubMed

  50. DRG - A NOVEL DEVELOPMENTALLY REGULATED GTP-BINDING PROTEIN Reviewed International coauthorship International journal

    T SAZUKA, Y TOMOOKA, Y IKAWA, M NODA, S KUMAR

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   Vol. 189 ( 1 ) page： 363 - 370   1992.11

    　More details

    Authorship：Lead author   Language：English   Publisher：ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS  

    DOI： 10.1016/0006-291X(92)91567-A

    Web of Science

    PubMed

  51. EXPRESSION OF DRG DURING MURINE EMBRYONIC-DEVELOPMENT

    T SAZUKA, M KINOSHITA, Y TOMOOKA, Y IKAWA, M NODA, S KUMAR

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   Vol. 189 ( 1 ) page： 371 - 377   1992.11

    　More details

    Language：English   Publisher：ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS  

    DOI： 10.1016/0006-291X(92)91568-B

    Web of Science

    PubMed

  52. IDENTIFICATION OF A DEVELOPMENTALLY REGULATED GENE IN THE MOUSE CENTRAL-NERVOUS-SYSTEM WHICH ENCODES A NOVEL PROLINE RICH PROTEIN

    T SAZUKA, Y TOMOOKA, S KATHJU, Y IKAWA, M NODA, S KUMAR

    BIOCHIMICA ET BIOPHYSICA ACTA   Vol. 1132 ( 3 ) page： 240 - 248   1992.10

    　More details

    Language：English   Publisher：ELSEVIER SCIENCE BV  

    A full length cDNA whose corresponding mRNA is down-regulated during the mouse embryonic brain development was isolated. The cDNA contains a single long open reading frame which could encode a protein with relative molecular mass of 41 kDa. The predicted gene product contains long stretches of prolines towards the NH2-terminus, followed by a leucine/proline rich region. The cDNA probe detected a number of mRNA species in Northern blot analysis. The reverse transcriptase-polymerase chain reaction analysis of mRNA from adult mouse tissues indicated that heart and testis expressed this gene (named NDPP-1) at relatively high levels, while lower levels of mRNA were detected in a number of other tissues. Expression of NDPP-1 was also detected in embryonic carcinoma and pheochromocytoma cell lines, but not in fibroblasts. The cDNA hybridized to genomic DNA from several vertebrates species in Southern blot analysis indicating interspecies conservation of this gene. The interesting pattern of expression of the NDPP-1 gene during mouse brain development and the structure of its putative protein product indicate that this gene may play an important biological role in the development of mouse central nervous system.

    Web of Science

  53. IDENTIFICATION OF A DEVELOPMENTALLY REGULATED GENE IN THE MOUSE CENTRAL-NERVOUS-SYSTEM WHICH ENCODES A NOVEL PROLINE RICH PROTEIN

    T SAZUKA, Y TOMOOKA, S KATHJU, Y IKAWA, M NODA, S KUMAR

    BIOCHIMICA ET BIOPHYSICA ACTA   Vol. 1132 ( 3 ) page： 240 - 248   1992.10

    　More details

    Language：English   Publisher：ELSEVIER SCIENCE BV  

    A full length cDNA whose corresponding mRNA is down-regulated during the mouse embryonic brain development was isolated. The cDNA contains a single long open reading frame which could encode a protein with relative molecular mass of 41 kDa. The predicted gene product contains long stretches of prolines towards the NH2-terminus, followed by a leucine/proline rich region. The cDNA probe detected a number of mRNA species in Northern blot analysis. The reverse transcriptase-polymerase chain reaction analysis of mRNA from adult mouse tissues indicated that heart and testis expressed this gene (named NDPP-1) at relatively high levels, while lower levels of mRNA were detected in a number of other tissues. Expression of NDPP-1 was also detected in embryonic carcinoma and pheochromocytoma cell lines, but not in fibroblasts. The cDNA hybridized to genomic DNA from several vertebrates species in Southern blot analysis indicating interspecies conservation of this gene. The interesting pattern of expression of the NDPP-1 gene during mouse brain development and the structure of its putative protein product indicate that this gene may play an important biological role in the development of mouse central nervous system.

    Web of Science

▼display all

To the head of MISC.▲

Presentations 171

  1. Development of UAV-based sorghum biomass estimation technology

    Kubota Chihiro, Nakata Tomohiro, Inoue Shun, Sazuka Takashi, Nishiuchi Shunsaku

    Abstracts of Meeting of the CSSJ  2024.3.29  CROP SCIENCE SOCIETY OF JAPAN

    　More details

    Event date： 2024.3

    Language：Japanese   Presentation type：Oral presentation (general)  

    DOI： 10.14829/jcsproc.257.0_107

    CiNii Research

  2. ジョンソングラス(Sorghum halepense)の頑健性を活用した新しいエネルギー作物育種の可能性

    岡田聡史、橋本舜平、山田千祥紀、中村（荒木）聡子、篠原（大前）梢、春日重光、佐塚隆志

    日本育種学会第145回講演会  2024.3.16 

    　More details

    Event date： 2024.3

    Language：Japanese   Presentation type：Poster presentation  

  3. 脱炭素社会構築を目指したジョンソングラスの遺伝資源調査

    山田千祥紀、橋本舜平、岡田聡史、中村（荒木）聡子、篠原（大前）梢、春日重光、佐塚隆志

    第30回日本育種学会中部地区談話会  2023.12.17 

    　More details

    Event date： 2023.12

    Presentation type：Poster presentation  

  4. スイートソルガムのスイート性のおもしろさと解析の難しさ International conference

    岡田聡史、中村（荒木）聡子、篠原（大前）梢、佐塚隆志

    第14回ソルガムワークショップ  2023.11.30  ソルガム研究会

    　More details

    Event date： 2023.11 - 2023.12

    Language：Japanese   Presentation type：Symposium, workshop panel (nominated)  

    Venue：名古屋  

  5. 6つの遺伝子でソルガムF1の雑種強勢を説明する International conference

    菊池樹、岡田聡史、橋本舜平、中村聡子、大前梢、荻野千秋、春日重光、佐塚隆志.

    第14回ソルガムワークショップ  2023.11.30  ソルガム研究会

    　More details

    Event date： 2023.11 - 2023.12

    Language：Japanese   Presentation type：Symposium, workshop panel (nominated)  

    Venue：名古屋  

  6. Genome-wide association study of the sorghum root system at the early growth stage International conference

    Okada S. Inukai Y. Zhenyu H, Nishiuchi S, Hashimoto S, Araki-Nakamura S, Ohmae-Shinohara K, Sazuka T

    2023.9.21 

    　More details

    Event date： 2023.9

    Language：English   Presentation type：Poster presentation  

  7. オーキシン輸送関連遺伝子の超優性で雑種強勢の一部を説明する試み

    橋本舜平、 中村(荒木)聡子、 篠原(大前)梢、 菊池樹、 三浦孝太郎、 川口秀夫、 春日重光、 佐塚隆志

    日本育種学会第141回講演会  2022.3.2  日本育種学会

    　More details

    Event date： 2023.9

    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：オンライン  

  8. ソルガム参照ゲノム配列を複数活用したトランスクリプトーム解析による搾汁液高糖性の解明

    岡田聡史、池崎未来、橋本舜平、菊池樹、中村（荒木）聡子、篠原（大前）梢、佐塚隆志

    日本育種学会第144回講演会  2023.9.17 

    　More details

    Event date： 2023.9

    Language：Japanese   Presentation type：Poster presentation  

  9. バイオマス作物研究：これまでとこれから Invited

    佐塚隆志

    日本育種学会第144回講演会  2023.9.16 

    　More details

    Event date： 2023.9

    Language：Japanese   Presentation type：Oral presentation (invited, special)  

  10. ソルガムF1品種の純系化育種：優性アレル集積系統の高バイオマス高糖性育種.

    菊池樹、岡田聡史、中村聡子、大前梢、荻野千秋、春日重光、佐塚隆志. ソルガムF1品種の純系化育種

    日本育種学会第144回講演会  2023.9.16 

    　More details

    Event date： 2023.9

    Language：Japanese   Presentation type：Oral presentation (general)  

  11. The sixth important locus for hybrid vigor in a Japanese F1 variety of sorghum International conference

    Okada S, Kikuchi T, Wake T, Hashimoto S, Araki-Nakamura S, Ohmae-Shinohara K, Miura K, Kawaguchi H, Ogino C, Kasuga S, Sazuka T

    Sorghum in the 21st Century  2023.6.6 

    　More details

    Event date： 2023.6

    Language：English   Presentation type：Poster presentation  

    Venue：Montpellier  

  12. UAV空撮画像を用いたソルガムバイオマス推定モデルの開発

    窪田千尋，中田智大，井上駿，佐塚隆志，西内俊策

    農業情報学会2024年度年次大会  2023.5 

    　More details

    Event date： 2023.5

    Presentation type：Oral presentation (general)  

  13. ソルガムの雑種強勢の一部をオーキシン輸送関連 遺伝子Dw3 の超優性効果で説明する試み

    橋本 舜平, 岡田 聡史, 中村（荒木） 聡 子, 大前（篠原） 梢, 三浦 孝太郎, 川口 秀夫, 荻野 千秋, 春日 重光, 佐塚 隆志

    日本育種学会第143回講演会  2023.3.18  日本育種学会

    　More details

    Event date： 2023.3

    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：静岡  

  14. ソルガムF1品種「天高」の雑種強勢に重要な第6の遺伝子

    菊池樹、岡田聡史、橋本舜平、中村聡子、大前梢、三浦孝太郎、川口秀夫、荻野千秋、春日重光、佐塚隆志

    日本育種学会第143回講演会  2023.3.18  日本育種学会

    　More details

    Event date： 2023.3

    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：静岡  

  15. ソルガムF1 品種「天高」の雑種強勢に重要な第6の遺伝子座の同定

    菊池樹、岡田聡史、橋本舜平、中村聡子、大前梢、三浦孝太郎、川口秀夫、荻野千秋、春日重光、佐塚隆志

    第29回日本育種学会中部地区談話会  2022.11.17  日本育種学会中部地区談話会

    　More details

    Event date： 2022.11

    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：オンライン  

  16. 天高の高バイオマス性を説明する第6の遺伝子

    菊池樹、岡田聡史、橋本舜平、中村聡子、篠原梢、三浦孝太郎、川口秀夫、荻野千秋、春日重光、佐塚隆志

    第13回ソルガムワークショップ  2022.11.11 

    　More details

    Event date： 2022.11

    Language：Japanese   Presentation type：Symposium, workshop panel (public)  

  17. 名大ソルガムパネルを用いた根系に関するゲノムワイド関連解析

    岡田聡史、胡振宇、犬飼義明、西内俊策、山内卓樹、中村 (荒木) 聡子、篠原(大前) 梢、川口秀夫、荻野千秋、三浦孝太郎、佐塚隆志

    第13回ソルガムワークショップ 

    　More details

    Event date： 2022.11

    Language：Japanese  

  18. ソルガム由来成分による微生物発酵促進作用に関する網羅的遺伝子発現解析

    川口秀夫、兵頭梓、吉原久美子、岡田聡史、佐塚隆志

    第13 回ソルガムワークショップ  2022.11.11  ソルガム研究会

    　More details

    Event date： 2022.11

    Language：Japanese   Presentation type：Symposium, workshop panel (public)  

    Venue：東京  

  19. 天高の高バイオマス性を説明する第6の遺伝子

    菊池樹、岡田聡史、橋本舜平、中村聡子、篠原梢、三浦孝太郎、川口秀夫、荻野千秋、春日重光、佐塚隆志

    第13回ソルガムワークショップ  2022.11.11 

    　More details

    Event date： 2022.11

    Language：Japanese   Presentation type：Symposium, workshop panel (public)  

  20. ソルガムの根系育種へ向けたゲノムワイド関連解析 International conference

    岡田聡史、胡振宇、犬飼義明、西内俊策、山内卓樹、中村（荒木）聡子、篠原（大前）梢、中村春貴、三浦孝太郎、春日重光、佐塚隆志

    日本育種学会第142回講演会  2022.9.24  日本育種学会

    　More details

    Event date： 2022.9

    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：帯広  

  21. UAVリモートセンシングによる高収量性ソルガムのバイオマス推定技術の開発

    Nakata T, Inoue S, Sazuka T, Nishiuchi S

    日本育種学会第141回講演会  2022.3.20 

    　More details

    Event date： 2022.3

  22. ソルガム胚乳高糖性変異体の原因遺伝子同定と育種利用の可能性

    橋本舜平、中村（荒木）聡子、中村春貴、篠原（大前）梢、三浦孝太郎、春日重光、佐塚隆志

    日本育種学会第141回講演会  2022.3.21  日本育種学会

    　More details

    Event date： 2022.3

    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：オンライン  

  23. 酸素制限条件におけるコリネ型細菌のアミノ酸代謝についてのメタボローム解析

    川口 秀夫、蓮沼 誠久、大西 康夫、佐塚 隆志、荻野 千秋、近藤 昭彦

    日本農芸化学会 2022年度大会  2022.3.17 

    　More details

    Event date： 2022.3

  24. 脱炭素社会構築に向けた実効性のある植物研究とは：エネルギー作物ソルガムの例 Invited

    佐塚隆志

    第37回資源植物シンポジウム・第13回植物ストレス科学シンポジウム  2022.2.25  岡山大学資源植物科学研究所

    　More details

    Event date： 2022.2

    Language：Japanese   Presentation type：Symposium, workshop panel (nominated)  

    Venue：オンライン  

  25. Biomass innovation by elucidating the principle of hybrid vigor Invited International conference

    Takashi SAZUKA

    JST connect webinar  2022.1.27  JST

    　More details

    Event date： 2022.1

    Language：English   Presentation type：Oral presentation (invited, special)  

    Venue：online  

  26. 脱炭素社会構築への貢献を目標とした新しい作物育種 Invited

    佐塚隆志

    近畿作物育種研究会シンポジウム  2021.12.11  近畿作物育種研究会

    　More details

    Event date： 2021.12

    Language：Japanese   Presentation type：Symposium, workshop panel (nominated)  

    Venue：オンライン  

  27. 雑種強勢の原理解明によるバイオマス技術革新　

    佐塚隆志、春日重光

    JSTキックオフイベントシンポジウム  2021.12.2  名古屋大学生物機能開発利用研究センター

    　More details

    Event date： 2021.12

    Language：Japanese   Presentation type：Symposium, workshop panel (nominated)  

    Venue：オンライン  

  28. Microbial monomer production from lignocellulosic biomass for bioplastic synthesis International conference

    Kawaguchi H, Hanh DD, Ohnishi Y, Sazuka T, Kaneko T, Ogino C, Kondo A

    The 26th Symposium of Young Asian Biological Engineers’ Community (YABEC 2021)  2021.11.19 

    　More details

    Event date： 2021.11

    Language：English   Presentation type：Oral presentation (general)  

  29. オーキシン輸送関連遺伝子の超優性で雑種強勢の一部を説明する試み

    橋本舜平、 中村(荒木)聡子、 篠原(大前)梢、 菊池樹、 三浦孝太郎、 川口秀夫、 春日重光、 佐塚隆志

    第12回ソルガムワークショップ  2021.11.17  ソルガム研究会

    　More details

    Event date： 2021.11

    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：オンライン  

  30. UAV 搭載マルチスペクトルカメラを用いた高収量性ソルガムのバイオマス推定技術の開発

    第28回育種学会中部地区談話会  2021.11.13  日本育種学会

    　More details

    Event date： 2021.11

    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：オンライン  

  31. ソルガムF1品種天高の部分的な超優性効果について

    橋本舜平、 中村(荒木)聡子、 篠原(大前)梢、 菊池樹、 三浦孝太郎、 川口秀夫、 春日重光、 佐塚隆志

    第28回育種学会中部地区談話会  2021.11.13  日本育種学会

    　More details

    Event date： 2021.11

    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：オンライン  

  32. ソルガム F₁ 雑種発現不均一領域の探索及び矮性遺伝子を活用したF1育種 Invited

    佐塚隆志

    理化学研究所科学技術ハブシンポジウム  2021.11.1  理化学研究所

    　More details

    Event date： 2021.11

    Language：Japanese   Presentation type：Symposium, workshop panel (nominated)  

    Venue：オンライン  

  33. 酸素制限による遺伝子組換えコリネ型細菌を用いた3-amino-4-hydroxybenzoic acid 発酵の促進作用

    川口 秀夫、蓮沼 誠久、大西 康夫、佐塚 隆志、荻野 千秋、近藤 昭彦

    第73回日本生物工学会大会  2021.10.28  日本生物工学会大会

    　More details

    Event date： 2021.10

    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：オンライン  

  34. UAV搭載マルチスペクトルカメラを用いた高収量性ソルガムのバイオマス推定技術の開発

    日本育種学会 第140回講演会  2021.9.24 

    　More details

    Event date： 2021.9

    Language：Japanese   Presentation type：Oral presentation (general)  

  35. ソルガム F₁ 品種「天高」の安定的種子生産に重要な種子親系統が有する矮性遺伝子の同定

    橋本舜平、 中村春貴、 中村（荒木） 聡子、 篠原（大前） 梢、 菊池樹、 三浦孝太郎、 川口秀夫、春日重光、 佐塚隆志

    日本育種学会 第140回講演会  2021.9.25  日本育種学会

    　More details

    Event date： 2021.9

    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：オンライン  

  36. Metabolome analysis-based design of a metabolic pathway in Corynebacterium glutamicum for 3-amino-4-hydroxybenzoic acid production under oxygen limitation International conference

    Kawaguchi H, Hasunuma T, Ohnishi Y, Sazuka T, Kondo A, Ogino C

    Metabolic Engineering 14  2021.7.13  World Microbe Forum

    　More details

    Event date： 2021.7

    Language：English   Presentation type：Poster presentation  

    Venue：on line  

  37. Amino acid metabolism of Corynebacterium glutamicum under oxygen limitation International conference

    Kawaguchi H, Hasunuma T, Ohnishi Y, Sazuka T, Kondo A, Ogino C

    World Microbe Forum 2021  2021.6.21  FEMS

    　More details

    Event date： 2021.6

    Presentation type：Poster presentation  

    Venue：on line  

  38. ソルガムF1品種「天高」の雑種強勢は優性説によって概ね必要十分に説明される

    和氣達朗、 橋本舜平、中村春貴、南山将輝、中村（荒木）聡子、篠原（大前）梢、纐纈永里子、岡村進之介、三浦孝太郎、川口秀夫、春日重光、佐塚隆志

    日本育種学会第139回講演会  2021.3.20  日本育種学会

    　More details

    Event date： 2021.3

    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：オンライン  

  39. 名大発新品種を社会実装する：新しいバリューチェーンの構築と実践 International conference

    佐塚隆志

    ソルガムワークショップ  2020.12.1  ソルガム研究会

    　More details

    Event date： 2020.12

    Language：English   Presentation type：Oral presentation (general)  

    Venue：オンライン  

  40. ソルガムF1品種の花粉親系統における下位節間の異常伸長に関する遺伝学的解析

    橋本舜平、和氣達朗、中村（荒木）聡子、山口未来、篠原（大前）梢、春日重光、佐塚隆志

    日本育種学会第138回講演会  2020.10.11  日本育種学会

    　More details

    Event date： 2020.10

    Language：Japanese   Presentation type：Poster presentation  

    Venue：オンライン  

  41. Mechanism of enhanced production of the γ-amino acid 3-amino-4-hydroxybenzoic acid under oxygen limitation by recombinant Corynebacterium glutamicum

    Kawaguchi, H., Miyazaki, S., Hasunuma, T., Ohnishi, Y., Sazuka, T., Kondo, A., Ogino, C.

    The 5th Annual Meeting of Living Systems Design Research  2020.3 

    　More details

    Event date： 2020.3

    Language：English   Presentation type：Oral presentation (general)  

    Venue：Okinawa  

  42. 作物ゲノム育種が持続可能な社会を創り出す Invited

    佐塚隆志

    第３回 名大・岐大農学シンポジウム  2020.2.3  東海国立大学機構

    　More details

    Event date： 2020.2

    Language：Japanese   Presentation type：Symposium, workshop panel (nominated)  

    Venue：岐阜  

  43. QTL Analyses for Hybrid Vigor: A Case Study of Sorghum F1 Variety “Tentaka” International conference

    Hashimoto, S., Araki-Nakamura, S., Wake, T., Yamaguchi, M., Shinohara-Ohmae, K., Miura, K., Kasuga, S., Sazuka T.

    2020.1.13 

    　More details

    Event date： 2020.1

    Language：English   Presentation type：Poster presentation  

    Venue：San Diego  

  44. ソルガムにおける温度依存的開花について International conference

    橋本舜平、手塚孝弘、横井修司、佐塚隆志

    第27回育種学会中部地区談話会  2019.11.30 

    　More details

    Event date： 2019.11

    Language：Japanese   Presentation type：Poster presentation  

  45. 「天高」種子親の短稈化に関与する新規矮性遺伝子について

    橋本舜平、中村（荒木）聡子、和氣達朗、篠原（大前）梢、中村春貴、三浦孝太郎、春日重光、佐塚隆志

    第10回ソルガムワークショップ  2019.11.19 

    　More details

    Event date： 2019.11

    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：岡山  

  46. ソルガム育種における栽培及び家畜飼料適性の検討

    毛利世那 、橋本舜平、中村(荒木) 聡子、 吉村文孝、篠原(大前) 梢、 西内俊作、佐塚隆志

    育種学会中部地区談話会  2019.11.19 

    　More details

    Event date： 2019.11

    Language：Japanese   Presentation type：Poster presentation  

    Venue：沼津  

  47. ソルガムの酵素糖化効率品種間差を決定する物理化学特性の探索

    川口秀夫、福本妙子、佐塚隆志

    第10回ソルガムワークショップ  2019.11.19 

    　More details

    Event date： 2019.11

    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：岡山  

  48. 「天高」の雑種強勢は主に優性説で説明される

    和氣達朗、橋本舜平、中村（荒木）聡子、篠原（大前）梢、中村春貴、三浦孝太郎、春日重光、佐塚隆志

    第10回ソルガムワークショップ  2019.11.19 

    　More details

    Event date： 2019.11

    Language：Japanese   Presentation type：Oral presentation (general)  

  49. 作物育種で持続可能な社会を創りだす Invited

    佐塚隆志

    日本農学会シンポジウム  2019.10.5  日本農学会

    　More details

    Event date： 2019.10

    Language：Japanese   Presentation type：Symposium, workshop panel (nominated)  

    Venue：東京  

  50. ソルガムF1品種「天高」後代のQTL解析で見出された４つの稈長制御遺伝子座について

    橋本舜平，中村（荒木）聡子，和氣達朗、佐々木碩哲、篠原（大前）梢，三浦孝太郎，春日重光，佐塚隆志

    日本育種学会第136回講演会  2019.9.7 

    　More details

    Event date： 2019.9

    Language：Japanese   Presentation type：Poster presentation  

  51. バイリファイナリーに最適な作物ゲノムを設計する

    佐塚隆志

    みえバイオリファイナリー研究会公開セミナー  2019.7.22  みえバイオリファイナリー研究会

    　More details

    Event date： 2019.7

    Language：Japanese   Presentation type：Symposium, workshop panel (nominated)  

    Venue：津  

  52. 胚乳高糖性を示す子実型ソルガム突然変異系統の解析

    橋本舜平、中村（荒木）聡子、篠原（大前）梢、田中 剛士、 中村 春貴、三浦孝太郎、春日重光、佐塚隆志

    日本育種学会第137回講演会  2019.3.29  日本育種学会

    　More details

    Event date： 2019.3

    Language：Japanese   Presentation type：Poster presentation  

    Venue：東京  

  53. ソルガム高バイオマスF1品種「天高」の稈径とバイオマス関連形質に関するQTL解析

    和氣達朗、中村（荒木）聡子、篠原（大前）梢、三浦孝太郎、春日重光、佐塚隆志

    日本育種学会第135回講演会  2019.3.17 

    　More details

    Event date： 2019.3

    Language：Japanese   Presentation type：Poster presentation  

  54. 作物ゲノムのデザイニング―社会と向き合う植物科学―

    佐塚隆志

    植物科学シンポジウム2018  2018.12.12 

    　More details

    Event date： 2018.12

    Language：Japanese   Presentation type：Symposium, workshop panel (nominated)  

  55. Caffeic acid production from lignocellulosic biomass by metabolically engineered Escherichia coli. International conference

    Kawaguchi, H., Sazuka, T., Katsuyama, Y., Teramoto, J., Nishida, K., Minami, H., Ogino, C., Ohnishi, Y., Kondo, A.

    YABEC 

    　More details

    Event date： 2018.11

    Language：English  

  56. Phenyllactic acid Production by recombinant Escherichia coli from sorghum bagasse: fermentation inhibition by the enzymatic hydrolysate

    Kawaguchi, H., Yoshihara, K., Hasunuma, T., Sazuka, T., Takaya, N., Ogino, C., Kondo, A.

    　More details

    Event date： 2018.6

    Language：English   Presentation type：Poster presentation  

    Venue：Atlanta  

  57. An agronomically important gene, Dw1, encodes a novel component of brassinosteroid signaling, and controls the cell proliferation in internodes. Sorghum in the 21st Century. International conference

    Sazuka, T., Hirano, K., Araki-Nakamura, S., Ohmae-Shinohara, K., Kawamura, M., Fujimoto, H., Yamaguchi, M., Fujii, A., Kasuga, S

    Sorghum in the 21st Century. 

    　More details

    Event date： 2018.4

    Language：English   Presentation type：Poster presentation  

  58. Heap: A Highly Sensitive and Accurate SNP Calling Tool with Low-Coverage High-Throughput Sequencing Data.

    Kobayashi, M., Ohyanagi, H., Takanashi, H., Asano, S., Kudo, T., Kajiya-Kanegae, H., Nagano, A.J., Tainaka, H., Tokunaga, T., Sazuka, T., Iwata, H., Tsutsumi, N. and Yano, K.

    Plant and Animal Genome XXV  2017.1.15 

    　More details

    Event date： 2017.1

    Language：English   Presentation type：Poster presentation  

    Venue：San Diego  

  59. Dw1, an Important Gene for Lodging Resistance and Mechanical Harvesting of Sorghum, Encodes a Novel Protein Involved in Cell Proliferation. Invited International conference

    Sazuka, T.

    2017.1.15 

    　More details

    Event date： 2017.1

    Language：English   Presentation type：Oral presentation (general)  

  60. Multi-Task Learning for Genomic Prediction Robust to Missing Data in Multi-Environmental Trials. International conference

    Hori, T., Fujimoto, M., Kajiya-Kanegae, H., Yamazaki, K., Hakoyama, T., Minamikawa, M., Nagano, A.J., Kobayashi, M., Sazuka, T., Yano, K. Tokunaga, T., Fujiwara, T., Tsutsumi, N., Iwata, H.

    Plant and Animal Genome XXV  2017.1.15 

    　More details

    Event date： 2017.1

    Language：English   Presentation type：Oral presentation (general)  

    Venue：San Diego  

  61. 資源循環利用を目指したソルガムの育種デザイン

    佐塚隆志

    第２回植物の栄養研究会  2016.9.2 

    　More details

    Event date： 2016.9

    Language：Japanese   Presentation type：Symposium, workshop panel (nominated)  

  62. F1 品種をデザインする

    佐塚隆志

    イネ分子遺伝学ワークショップ  2016.7.5 

    　More details

    Event date： 2016.7

    Language：Japanese   Presentation type：Symposium, workshop panel (nominated)  

    Venue：名古屋  

  63. 多形質へのゲノミックセレクション手法の開発：マルチタスク学習の応用

    堀智明、小林正明、大柳一、高梨秀樹、永野惇、徳永毅、佐塚隆志、矢野健太郎、堤伸浩、岩田洋佳

    日本育種学会第128回講演会 

    　More details

    Event date： 2015.9

    Language：Japanese   Presentation type：Oral presentation (general)  

    Country：Japan  

  64. 画像解析で定量化されたソルガム種子形態のゲノミック予測とゲノムワイド関連解析

    坂本莉沙、鐘ケ江弘美、野下浩司、石森元幸、小林正明、高梨秀樹、永野惇、徳永毅、佐塚隆志、矢野健太郎、堤伸浩、岩田洋佳

    日本育種学会第128回講演会 

    　More details

    Event date： 2015.9

    Language：Japanese   Presentation type：Poster presentation  

    Country：Japan  

  65. 形質調査およびジェノタイピングの大規模情報を統合した圃場情報管理システム整備

    浅野さとみ、釜付香、大柳一、小林正明、鐘ケ江弘美、高梨秀樹、徳永毅、佐塚隆志、岩田洋佳、堤伸浩、矢野健太郎

    日本育種学会第128回講演会 

    　More details

    Event date： 2015.9

    Language：Japanese   Presentation type：Poster presentation  

    Country：Japan  

  66. 形質調査およびジェノタイピングの大規模情報を統合した圃場情報管理システム整備

    浅野さとみ、釜付香、大柳一、小林正明、鐘ケ江弘美、高梨秀樹、徳永毅、佐塚隆志、岩田洋佳、堤伸浩、矢野健太郎

    日本育種学会第128回講演会 

    　More details

    Event date： 2015.9

    Language：Japanese   Presentation type：Oral presentation (general)  

    Country：Japan  

  67. 画像解析で定量化されたソルガム種子形態のゲノミック予測とゲノムワイド関連解析

    坂本莉沙、鐘ケ江弘美、野下浩司、石森元幸、小林正明、高梨秀樹、永野惇、徳永毅、佐塚隆志、矢野健太郎、堤伸浩、岩田洋佳

    日本育種学会第128回講演会 

    　More details

    Event date： 2015.9

    Language：Japanese   Presentation type：Oral presentation (general)  

    Country：Japan  

  68. ソルガムリファレンスパネルの全ゲノム配列を利用した遺伝子型予測

    鐘ケ江弘美、望月孝子、神沼英里、南川舞、小林正明、豊島裕美、大柳一、高梨秀樹、永野惇、徳永毅、佐塚隆志、矢野健太郎、中村保一、堤伸浩、岩田洋佳

    日本育種学会第127回講演会 

    　More details

    Event date： 2015.3

    Language：Japanese   Presentation type：Oral presentation (general)  

    Country：Japan  

  69. スイートソルガムの高糖性に関するQTL解析

    倉見慶二郎、中村聡子、山口未来、市川悦子、篠原梢、伊藤裕介、呉健忠、米丸淳一、春日重光、松岡信、北野英己、佐塚隆志

    日本育種学会第127回講演会 

    　More details

    Event date： 2015.3

    Language：Japanese   Presentation type：Poster presentation  

    Country：Japan  

  70. ソルガム高バイオマスF1品種「天高」の雑種強勢の約半分は優性説で説明される

    山口未来、中村（荒木）聡子、伊藤裕介、篠原（大前）梢、松岡信、北野英己、春日重光、佐塚隆志

    日本植物生理学会第56回年会 

    　More details

    Event date： 2015.3

    Language：Japanese   Presentation type：Poster presentation  

    Country：Japan  

  71. 高バイオマスソルガムF1系統の光合成特性とバイオマス増産の解析

    田副雄士、牧野周、佐塚隆志、平野恒、北野英己、池内雅裕、遠藤剛、齊藤知恵子、福田裕穂

    日本植物生理学会第56回年会 

    　More details

    Event date： 2015.3

    Language：Japanese   Presentation type：Poster presentation  

    Country：Japan  

  72. バイオエネルギー作物・ソルガムテーラーメード育種に向けた高速ジェノタイピング技術研究開発の現状

    大柳一、高野知之、高梨秀樹、鐘ヶ江 弘美、浅野 さとみ、尾崎崇一、工藤 徹、永野惇、田井中均、徳永毅、佐塚隆志、岩田洋佳、堤伸浩、矢野健太郎

    日本植物生理学会第56回年会 

    　More details

    Event date： 2015.3

    Language：Japanese   Presentation type：Oral presentation (general)  

    Country：Japan  

  73. Heap: 次世代シーケンサー・データに基づく高感度SNP 検出ツール

    小林正明、大柳一、豊島裕美、高梨秀樹、鐘ヶ江弘美、永野惇、田井中均、徳永毅、佐塚隆志、岩田洋佳、堤伸浩、矢野健太郎

    日本植物生理学会第56回年会 

    　More details

    Event date： 2015.3

    Language：Japanese   Presentation type：Oral presentation (general)  

    Country：Japan  

  74. Construction of a sorghum HapMap for genomics-assisted breeding

    　More details

    Event date： 2014.9

    Language：Japanese   Presentation type：Oral presentation (general)  

    Country：Japan  

  75. 強い雑種強勢を示すソルガムF1品種「天高」の後代集団を用いたQTL解析

    山口未来、中村（荒木）聡子、伊藤裕介、篠原（大前）梢、松岡信、北野英己、春日重光、佐塚隆志

    日本育種学会第126回講演会 

    　More details

    Event date： 2014.9

    Language：Japanese   Presentation type：Poster presentation  

    Country：Japan  

  76. 無人ヘリコプターを用いた高速フェノタイピングシステムの開発

    渡辺翔、新井啓吾、宇佐美昌樹、郭威、鐘ケ江弘美、南川舞、箱山雅生、山崎清志、高梨秀樹、藤本優、佐塚隆志、徳永毅、杉浦綾、二宮正士、藤原徹、堤伸浩、岩田洋佳

    日本育種学会第126回講演会 

    　More details

    Event date： 2014.9

    Language：Japanese   Presentation type：Oral presentation (general)  

    Country：Japan  

  77. ソルガム育種で使われている矮性遺伝子dw1とそのイネオルソログ遺伝子の解析

    藤本榛香、中村（荒木）聡子、篠原（大前）梢、春日重光、伊藤裕介、松岡信、北野英己、佐塚隆志

    日本育種学会第126回講演会 

    　More details

    Event date： 2014.9

    Language：Japanese   Presentation type：Poster presentation  

    Country：Japan  

  78. Heap：ゲノミックセレクションやゲノムワイド関連解析のための系統間 SNPs 検出ツール

    小林正明、大柳一、豊島裕美、高梨秀樹、永野惇、田井中均、徳永毅、佐塚隆志、岩田洋佳、堤伸浩、矢野健太郎

    日本育種学会第126回講演会 

    　More details

    Event date： 2014.9

    Language：Japanese   Presentation type：Oral presentation (general)  

    Country：Japan  

  79. ホウキモロコシ（Sorghum vulgare）の穂型に関する遺伝学的解析

    保崎翼、中村（荒木）聡子、松岡信、北野英己、佐塚隆志

    日本育種学会第126回講演会 

    　More details

    Event date： 2014.9

    Language：Japanese   Presentation type：Oral presentation (general)  

    Country：Japan  

  80. バイオマス糖化液由来成分が大腸菌のフェニル乳酸発酵に与える影響

    川口秀夫、寺村浩、中村聡子、荻野千秋、原清敬、蓮沼誠久、老沼研一、高谷直樹、平野恒、佐塚隆志、北野英己、近藤昭彦

    第66回日本生物工学会大会 

    　More details

    Event date： 2014.9

    Language：Japanese   Presentation type：Oral presentation (general)  

    Country：Japan  

  81. バイオエネルギー作物・ソルガム高速育種への取り組み

    大柳一 、小林正明、豊島裕美、高野知之、高梨秀樹、永野惇、田井中均、徳永毅、佐塚隆志、岩田洋佳、堤伸浩、矢野健太郎

    第32回植物細胞分子生物学会 

    　More details

    Event date： 2014.8

    Language：Japanese   Presentation type：Oral presentation (general)  

    Country：Japan  

  82. 高速シーケンスデータから高精度かつ多量な系統間SNPsを検出するツール”Heap”

    小林正明、大柳一、豊島裕美、高梨秀樹、永野惇、田井中均、徳永毅、佐塚隆志、岩田洋佳、堤伸浩、矢野健太郎

    第32回植物細胞分子生物学会 

    　More details

    Event date： 2014.8

    Language：Japanese   Presentation type：Oral presentation (general)  

    Country：Japan  

  83. ソルガムの高バイオマスF1品種「天高」の自殖F2集団を用いたQTL 解析

    山口未来、伊藤裕介、米丸淳一、中村（荒木）聡子、篠原（大前）梢、松岡信、北野英己、春日重光、佐塚隆志

    日本育種学会第125回講演会 

    　More details

    Event date： 2014.3

    Language：Japanese   Presentation type：Poster presentation  

    Country：Japan  

  84. ソルガムにおける特定組み合わせ能力のGWAマッピング

    高梨秀樹、阿部竜之、小野木章雄、大柳一、小林正明、豊島裕美、矢野健太郎、田井中均、永野惇、徳永毅、佐塚隆志、岩田洋佳、堤伸浩

    日本育種学会第125回講演会 

    　More details

    Event date： 2014.3

    Language：Japanese   Presentation type：Oral presentation (general)  

    Country：Japan  

  85. ソルガムの高速ジェノタイピングにおけるバイオインフォマティクス手法の開発・改良

    大柳一、小林正明、豊島裕美、高野知之、高梨秀樹、永野惇、田井中均、徳永毅、佐塚隆志、岩田洋佳、堤伸浩、矢野 健太郎

    日本育種学会第125回講演会 

    　More details

    Event date： 2014.3

    Language：Japanese   Presentation type：Oral presentation (general)  

    Country：Japan  

  86. バイオエネルギー作物・ソルガムのテーラーメード育種技術開発を目指した高速ジェノタイピング手法の検討

    大柳一、小林正明、豊島裕美、高野知之、高梨秀樹、永野惇、田井中均、徳永毅、佐塚隆志、岩田洋佳、堤伸浩、矢野健太郎

    第55回日本植物生理学会年会 

    　More details

    Event date： 2014.3

    Language：Japanese   Presentation type：Oral presentation (general)  

    Country：Japan  

  87. ソルガムにおけるバイオマス関連重要形質のデザイン

    佐塚隆志

    第55回日本植物生理学会年会 

    　More details

    Event date： 2014.3

    Language：Japanese   Presentation type：Oral presentation (general)  

    Country：Japan  

  88. 同時糖化発酵による希硫酸前処理バイオマスからのフェニル乳酸生産

    川口秀夫、寺村浩、中村聡子、荻野千秋、老沼研一、高谷直樹、平野恒、佐塚隆志、北野英己、近藤昭彦

    日本農芸化学会2014年度大会 

    　More details

    Event date： 2014.3

    Language：Japanese   Presentation type：Oral presentation (general)  

    Country：Japan  

  89. バイオリファイナリー利用に向けたソルガムのゲノム育種の挑戦

    佐塚隆志

    平成２５年度岡山大学資源植物科学研究所学術講演会 

    　More details

    Event date： 2014.2

    Language：Japanese   Presentation type：Oral presentation (invited, special)  

    Country：Japan  

  90. Development of a Bioinformatics Pipeline for Detection of Genome-Wide SNPs. International conference

    Kobayashi, M., Ohyanagi, H., Toyoshima, H., Takano, T., Takanashi, H., Nagano, A.J., Tainaka, H., Tokunaga,T., Sazuka, T., Iwata, H., Tsutsumi, N. and Yano, K.

    Plant and Animal Genome XXII 

    　More details

    Event date： 2014.1

    Language：English   Presentation type：Poster presentation  

    Country：United States  

  91. Genomics-assisted breeding for sorghum improvement.

    　More details

    Event date： 2013.11

    Language：English   Presentation type：Oral presentation (invited, special)  

    Country：Japan  

  92. バイオリファイナリー利用を目指した次世代型ソルガムの育種開発

    佐塚隆志

    奈良先端科学技術大学院大学セミナー 

    　More details

    Event date： 2013.11

    Language：Japanese   Presentation type：Oral presentation (invited, special)  

    Country：Japan  

  93. ソルガムの矮性遺伝子Dw1とDw3の遺伝学的相互作用の解析

    藤本榛香、伊藤裕介、中村聡子、篠原（大前）梢、浜田和行、春日重光、松岡信、北野英己、佐塚隆志

    第21回育種学会中部地区談話会 

    　More details

    Event date： 2013.11

    Language：English   Presentation type：Poster presentation  

    Country：Japan  

  94. An analysis of bending dwarf (bdw) mutants of sorghum: Gibberellin-controlled symmetrical cell division in the culm and im and its in¬fluence on sorghum breeding.

    　More details

    Event date： 2013.10

    Language：Japanese   Presentation type：Oral presentation (general)  

    Country：Japan  

  95. 作物ゲノム育種を支援するためのハイスループット表現型計測システム

    岩田洋佳、佐々木敦司、小林正明、大柳一、 矢野健太郎、佐塚隆志、徳永毅、堤 伸浩

    日本育種学会第124回講演会 

    　More details

    Event date： 2013.10

    Language：Japanese   Presentation type：Oral presentation (general)  

    Country：Japan  

  96. ゲノムワイド SNPマーカーを用いたソルガム遺伝資源の遺伝的多様性評価

    阿部竜之、高梨秀樹、永野惇、大柳一、小林正明、田井中均、 佐々木敦司、徳永毅、佐塚隆志、吉村和紗、矢野健太郎、 岩田洋佳、堤伸一

    日本育種学会第124回講演会 

    　More details

    Event date： 2013.10

    Language：Japanese   Presentation type：Oral presentation (general)  

    Country：Japan  

  97. バイオエネルギー作物・ソルガムの高速ジェノタイピングにおけるバイオインフォマティクス手法の検討・開発

    大柳一、小林正明、豊島裕美、高野知之、高梨秀樹、永野 惇、田井中均、徳永毅、佐塚隆志、岩田洋佳、堤伸浩、矢野健太郎

    日本育種学会第124回講演会 

    　More details

    Event date： 2013.10

    Language：Japanese   Presentation type：Oral presentation (general)  

    Country：Japan  

  98. 高速ジェノタイピングを利用したソルガムのテーラーメード育種

    高梨秀樹、 阿部竜之、 大柳一、 小林正明、矢野健太郎、田井中均、永野惇、 徳永毅、佐塚隆志、 岩田洋佳、堤伸浩

    日本育種学会第124回講演会 

    　More details

    Event date： 2013.10

    Language：Japanese   Presentation type：Oral presentation (general)  

    Country：Japan  

  99. CO2削減および資源化に最適化した高バイオマスソルガムの育種開発

    佐塚隆志

    第２回バイオマスリファイナリー研究会  2013.9.27 

    　More details

    Event date： 2013.9

    Language：Japanese   Presentation type：Oral presentation (invited, special)  

    Country：Japan  

  100. RAD-Seq をゲノミックセレクションに利用する：作物育種の大幅な加速化に向けて

    岩田洋佳、高梨秀樹、小林正明、大柳一、田井中均、徳永毅、佐塚隆志、矢野健太郎、堤伸浩

    日本植物学会第77回大会 

    　More details

    Event date： 2013.9

    Language：Japanese   Presentation type：Oral presentation (general)  

    Country：Japan  

  101. 易糖化性を示すイネの探索

    寺村浩、平野恒、松田史生、荻野千秋、佐塚隆志、北野英己、近藤昭彦

    第31回植物細胞分子生物学会 

    　More details

    Event date： 2013.9

    Language：Japanese   Presentation type：Oral presentation (general)  

    Country：Japan  

  102. バイオエネルギー作物・ソルガムのテーラーメード育種技術開発を目指したRAD-Seqによる高速ジェノタイピング手法の検討

    大柳一、小林正明、高野知之、高梨秀樹、永野惇、田井中均、徳永毅、佐塚隆志、岩田洋佳、堤伸浩、矢野健太郎

    第31回植物細胞分子生物学会 

    　More details

    Event date： 2013.9

    Language：Japanese   Presentation type：Oral presentation (general)  

    Country：Japan  

  103. RAD-Seq が切り拓く植物研究—栽培種から野生種まで

    岩田洋佳、高梨秀樹、小林正明、大柳一、田井中均、徳永毅、佐塚隆志、矢野健太郎、堤伸浩

    日本植物学会第77回大会 

    　More details

    Event date： 2013.9

    Language：Japanese   Presentation type：Oral presentation (general)  

    Country：Japan  

  104. 天高・風立の稈長を説明する遺伝学モデル

    佐塚隆志

    第4回ソルガムワークショップ 

    　More details

    Event date： 2013.6

    Language：Japanese   Presentation type：Oral presentation (general)  

    Country：Japan  

  105. ゲノミクスから育種へ：バイオリファイナリー利用に向けたソルガムのゲノム育種の挑戦

    佐塚隆志

    平成２５年度岡山大学資源植物科学研究所学術講演会  2013.2.18  岡山大学

    　More details

    Event date： 2013.2

    Language：Japanese   Presentation type：Symposium, workshop panel (nominated)  

    Venue：倉敷  

  106. An Overview of Gibberellin Biosynthetic Genes, Their Related Mutants and the Specific Phenotype in Sorghum. International conference

    Reynante L Ordonio, Ito, Y., Hatakeyama, A., Shinohara-Ohmae, K., Xian-Jun Song, Kasuga, S., Yonemaru, J., Tokunaga, T., Kitano, H., Matsuoka, M., Sazuka, T.

    Plant and Animal Genome XXI 

    　More details

    Event date： 2013.1

    Language：English   Presentation type：Oral presentation (general)  

    Country：United States  

  107. 高バイオマス高糖性ソルガムの育種開発

    佐塚隆志

    2012年植物科学シンポジウム 

    　More details

    Event date： 2012.12

    Language：Japanese   Presentation type：Symposium, workshop panel (public)  

    Country：Japan  

  108. エネルギー作物ソルガムの育種開発とその利活用の可能

    佐塚隆志

    第２３回バイオテクノロジーシンポジウム 

    　More details

    Event date： 2012.11

    Language：Japanese   Presentation type：Symposium, workshop panel (public)  

    Country：Japan  

  109. 高糖性高バイオマスソルガムの育種開発とその利活用の可能性

    佐塚隆志

    第23回バイオテクノロジーシンポジウム 

    　More details

    Event date： 2012.11

    Language：Japanese   Presentation type：Oral presentation (keynote)  

    Country：Japan  

  110. ソルガム育種に用いられた矮性遺伝 子の遺伝学的解析

    伊藤 裕介, 長江理恵, 篠原（大前） 梢, 春日重光, 米丸淳一, オル ドーニョ レイナンテ, 徳永毅, 北野英己, 松岡信, 佐塚 隆志

    日本育種学会第122回講演会 

    　More details

    Event date： 2012.9

    Language：Japanese   Presentation type：Oral presentation (general)  

    Country：Japan  

  111. スイートソルガム (Sorghum bicolor L. Moench)のジベレ リン関連変異体の表現系と遺伝学的解析

    レイナンテオルドーニョ, 畠山麻子, 大前梢, 春日重光, 山村 卓也, 徳永毅, 北野英己, 松岡信, 佐塚隆志

    日本育種学会第121回講演会 

    　More details

    Event date： 2012.3

    Language：Japanese   Presentation type：Oral presentation (general)  

    Country：Japan  

  112. Towards the Systematic Mutagenesis of Sweet Sorghum Genome by Gamma Ray: Screening and Analysis of Dwarf and Slender Mutants. International conference

    Plant and Animal Genome XX. 

    　More details

    Event date： 2012.1

    Language：English   Presentation type：Oral presentation (general)  

    Country：Japan  

  113. Identification and phenotypic analysis of gibberellin-related mutants of sweet sorghum (Sorghum bicolor L. Moench) International conference

    Reynante L. Ordonio, Asako Hatakeyama, Shigemitsu Kasuga, Yusuke Ito, Takuya Yamamura, Tsuyoshi Tokunaga, Hidemi Kitano, Makoto Matsuoka, and Takashi Sazuka

    　More details

    Event date： 2011.12

    Language：English   Presentation type：Oral presentation (general)  

    Country：Japan  

  114. ガンマ線によるソルガム突然変異体の作出と解析

    佐塚　隆志

    第50回ガンマーフィールドシンポジウム 

    　More details

    Event date： 2011.7

    Language：Japanese   Presentation type：Symposium, workshop panel (nominated)  

    Venue：水戸   Country：Japan  

  115. Systematic mutagenesis of sweet sorghum - Identification of thephenotypic orthologs of rice dwarf mutants International conference

    Ordonio, L.R., Yamamura, T., Tokunaga, T., Matsumoto, T., Kitano, H., Matsuoka, M. and Sazuka, T.

    Plant and Animal Genome XIX 

    　More details

    Event date： 2011.1

    Language：English   Presentation type：Poster presentation  

    Country：Japan  

  116. Systematic mutagenesis of sweet sorghum-Comparison to rice dwarf mutants and identification of phenotypic orthologs. International conference

    Ordonio, L.R., Yamamura, T., Tokunaga, T., Matsumoto, T., Kitano, H., Matsuoka, M., and Sazuka, T.

    Cold Spring Harbor Asia Conference 

    　More details

    Event date： 2010.10

    Language：English   Presentation type：Poster presentation  

  117. ソルガムの節間伸長に関するQTL解析

    長江理恵、大前梢、山村卓也、米丸淳一、春日重光、松本隆、呉健忠、北野英己、松岡信、佐塚隆志

    日本育種学会第118回講演会 

    　More details

    Event date： 2010.9

    Language：Japanese   Presentation type：Oral presentation (general)  

    Country：Japan  

  118. ソルガムの矮性及び高糖性のQTL解析と突然変異体の活用.

    佐塚隆志

    第一回ソルガムワークショップ 

    　More details

    Event date： 2010.6

    Language：Japanese   Presentation type：Oral presentation (general)  

    Country：Japan  

  119. Towards the systematic mutagenesis of sweet sorghum genome induced by gamma-ray: screening dwarf and slender mutants and analysis of the candidate gene.

    　More details

    Event date： 2010.3

    Language：Japanese   Presentation type：Oral presentation (general)  

    Country：Japan  

  120. Induction of sweet sorghum (Sorghum bicolor (L.) Moench) mutants by gamma ray and analysis the corresponding genes.

    　More details

    Event date： 2009.12

    Language：English   Presentation type：Oral presentation (general)  

    Country：Japan  

  121. チオレドキシンはカルシウム依存キナーゼをレドックス調節する

    中西華代、佐塚隆志、森仁志、久堀徹

    第49回日本植物生理学会年会 

    　More details

    Event date： 2008.3

    Language：Japanese   Presentation type：Oral presentation (general)  

    Country：Japan  

  122. イネDCL1が変異した棍棒状胚突然変異体club-shaped embryo1の解析

    今村 講平, 松岡 信, 北野 英己, 佐塚 隆志

    日本育種学会第112回講演会 

    　More details

    Event date： 2007.9

    Language：Japanese   Presentation type：Oral presentation (general)  

    Country：Japan  

  123. OsDICER-LIKE1に変異を持つイネ胚発生突然変異体club-shaped embryo1の解析

    今村講平，松岡信，北野英己，佐塚隆志

    イネ分子遺伝学ワークショップ 

    　More details

    Event date： 2007.7

    Language：Japanese   Presentation type：Oral presentation (general)  

    Country：Japan  

  124. OsDICER-LIKE1が機能欠失変異したイネ棍棒状胚突然変異体の解析

    今村講平，今井華子，松岡信，北野英己，佐塚隆志

    第48回日本植物生理学会年会 

    　More details

    Event date： 2007.3

    Language：Japanese   Presentation type：Oral presentation (general)  

    Country：Japan  

  125. チオレドキシン標的候補として細胞膜アンカー型信号伝達分子を同定した

    中西華代 　他

    第48回日本植物生理学会年会 

    　More details

    Event date： 2007.3

    Language：Japanese   Presentation type：Oral presentation (general)  

    Country：Japan  

  126. イネ棍棒状胚突然変異体cle1の原因遺伝子の単離と解析

    佐塚隆志

    国立遺伝学研究所研究集会 「イネの多様性を巡る諸問題」 

    　More details

    Event date： 2006.12

    Language：Japanese   Presentation type：Oral presentation (invited, special)  

    Country：Japan  

  127. イネ棍棒状胚突然変異体の原因遺伝子OsDICER-LIKE1の同定と解析

    今村 講平, 今井 華子, 松岡 信, 北野 英己, 佐塚 隆志

    日本分子生物学2006フォーラム 

    　More details

    Event date： 2006.12

    Language：Japanese   Presentation type：Oral presentation (general)  

    Country：Japan  

  128. イネにおけるオーキシン生合成と機能解析

    佐塚隆志

    イネ遺伝学・分子生物学ワークショップ2006 

    　More details

    Event date： 2006.7

    Language：Japanese   Presentation type：Oral presentation (general)  

    Country：Japan  

  129. イネ突然変異体を用いたオーキシンの機能解析

    佐塚隆志、神谷紀子、西村岳志、森中洋一、小柴共一、北野英己、松岡信

    日本農芸化学会2006年度大会 

    　More details

    Event date： 2006.3

    Language：Japanese   Presentation type：Oral presentation (invited, special)  

    Country：Japan  

  130. イネ棍棒状胚突然変異体の発生遺伝学的解析

    今村講平　他

    日本育種学会第107,108回講演会 

    　More details

    Event date： 2005.8

    Language：Japanese   Presentation type：Oral presentation (general)  

    Country：Japan  

  131. 矮性で葉身先端が丸みを帯びるイネの形態異常突然変異体 dwarf and gladius leaf 1(dgl1) の解析

    小森園正彦他

    イネ・シロイヌナズナ合同ワークショップ2005 

    　More details

    Event date： 2005.7

    Language：Japanese   Presentation type：Oral presentation (invited, special)  

    Country：Japan  

  132. イネYUCCA遺伝子の発現とオーキシン合成部位

    山本優子、神谷紀子、佐塚隆志、北野英巳、松岡信

    第46回日本植物生理学会年会 

    　More details

    Event date： 2005.3

    Language：Japanese   Presentation type：Oral presentation (general)  

    Country：Japan  

  133. イネ棍棒状胚突然変異体の遺伝解析.

    今村講平 、佐塚隆志 、今井華子 、松岡信 、北野英己

    育種学会中部地区談話会 

    　More details

    Event date： 2004.12

    Language：Japanese   Presentation type：Oral presentation (general)  

    Country：Japan  

  134. 葉身に形態異常を伴うイネ矮性突然変異体 dwarf and abnormal leaf morphology 1 (dal1)の原因遺伝子の単離と解析

    佐塚隆志、小森園正彦、上口（田中）美弥子、北野英己、芦苅基行、松岡信

    第45回日本植物生理学会年会 

    　More details

    Event date： 2004.12

    Language：Japanese   Presentation type：Oral presentation (general)  

    Country：Japan  

  135. 葉身先端が丸みをおびるイネ矮性突然変異体の解析

    佐塚隆志

    国立遺伝学研究所研究集会 

    　More details

    Event date： 2004.12

    Language：Japanese   Presentation type：Oral presentation (general)  

    Country：Japan  

  136. シロイヌナズナ膜タンパク質の網羅的解析

    "佐塚隆志,気多澄江,白武勝裕,山木昭平,柴田大輔"

    日本植物生理学会第43回シンポジウム 

    　More details

    Event date： 2003.3

    Language：Japanese   Presentation type：Oral presentation (general)  

    ", 奈良"

  137. ゲノム情報を活用した植物プロテオーム解析の実際 -シアノバクテリアとシロイヌナズナを例として-.

    佐塚隆志

    大阪大学蛋白質研究所セミナー「プロテオーム・ペプチドーム最前線 -解析法と実際-」 

    　More details

    Event date： 2003.2

    Language：Japanese   Presentation type：Oral presentation (invited, special)  

    Country：Japan  

    ", 大阪"

  138. 植物ゲノミクスからプロテオミクスへ: -ラン藻とシロイヌナズナのプロテオーム解析-.

    佐塚隆志

    名古屋大学生物分子応答研究センターセミナー 

    　More details

    Event date： 2002.10

    Language：Japanese   Presentation type：Oral presentation (invited, special)  

    Country：Japan  

    ", 名古屋"

  139. ラン藻及びシロイヌナズナのプロテオミクス

    "佐塚隆志,柴田大輔"

    第二回蛋白科学会年会島津バイオテク・ランチョンセミナー「プロテオーム解析の現状と将来」 

    　More details

    Event date： 2002.6

    Language：Japanese   Presentation type：Oral presentation (invited, special)  

    ", 名古屋"

  140. 微生物・植物ゲノムとプロテオーム解析

    佐塚隆志

    東京大学医科学研究所セミナー 

    　More details

    Event date： 2002.5

    Language：Japanese   Presentation type：Oral presentation (invited, special)  

    ", 東京"

  141. シロイヌナズナ液胞膜のプロテオミクス

    "佐塚隆志,柴田大輔"

    研究集会「植物の生存戦略における液胞機能の総合的理解」 

    　More details

    Event date： 2001.11

    Language：Japanese   Presentation type：Oral presentation (invited, special)  

    Country：Japan  

  142. Proteomics of Arabidopsis thaliana tonoplast-enriched membrane fraction. International conference

    "7th RIKEN Conference "Plant endomembranes: biogenesis, traffic and dynamics"" 

    　More details

    Event date： 2001.10

    Language：English   Presentation type：Poster presentation  

    Country：Japan  

  143. Proteome analysis of Arabidopsis thaliana tonoplast-enriched low-density membrane particles. International conference

    International Proteomics Conference (IPC 2001) 

    　More details

    Event date： 2001.9

    Language：English   Presentation type：Oral presentation (invited, special)  

    Canberra Australia

  144. シロイヌナズナのゲノム情報を活用した膜タンパクのプロテオーム研究

    "佐塚隆志,白武勝裕,山木昭平,柴田大輔"

    日本植物細胞分子生物学会シンポジウム 

    　More details

    Event date： 2001.7

    Language：Japanese   Presentation type：Oral presentation (invited, special)  

    ", 東京"

  145. シロイヌナズナ液胞膜タンパクの包括的解析

    "佐塚隆志,柴田大輔"

    研究集会「植物の生存戦略における液胞機能の総合的理解」 

    　More details

    Event date： 2001.1

    Language：Japanese   Presentation type：Oral presentation (invited, special)  

    Country：Japan  

  146. ゲノムからポストゲノムへ:-ラン藻プロテオーム解析をモデル・ケースとして-.

    佐塚隆志

    茨城大学農学部特別講義 

    　More details

    Event date： 2000.12

    Language：Japanese   Presentation type：Oral presentation (invited, special)  

    ", 阿見"

  147. 単細胞性ラン藻 Synechocystis sp. strain PCC6803 及び糸状性ラン藻 Anabaena sp. strain PCC7120 の比較プロテオーム解析

    佐塚隆志

    第51回日本電気泳動学会総会秋季討論会シンポジウム 

    　More details

    Event date： 2000.11

    Language：Japanese   Presentation type：Oral presentation (invited, special)  

    ", 松山"

  148. Cyanobacterial proteomics in Kazusa DNA research institute. International conference

    "Korea Basic Science Institute Seminar," 

    　More details

    Event date： 2000.10

    Language：English   Presentation type：Oral presentation (invited, special)  

    " Taejon Korea, Republic Of"

  149. Towards the comparative proteomics of cyanobacteria: Synechocystis sp. strain PCC6803 and Anabaena sp. strain PCC7120. International conference

    "Korea Genome Organization Symposium "Recent Progress in Genomics, Proteomics and Bioinfomatics"" 

    　More details

    Event date： 2000.10

    Language：English   Presentation type：Oral presentation (invited, special)  

    " Seoul Korea, Republic Of"

  150. Comparative proteomics of cyanobacteria: Synechocystis sp. strain PCC6803 and Anabaena sp. strain PCC7120. International conference

    From Genome to Proteome; 4th Siena 2D Electrophoresis Meeting 

    　More details

    Event date： 2000.9

    Language：English   Presentation type：Oral presentation (general)  

    Siena Italy

  151. ラン藻 Synechocystis sp. strain PCC6803及びAnabaena sp. strain PCC7120のプロテオーム解析

    佐塚隆志

    蛋白合同年会東京2000シンポジウム 

    　More details

    Event date： 2000.6

    Language：Japanese   Presentation type：Oral presentation (invited, special)  

    Country：Japan  

    ", 東京"

  152. ラン藻ゲノム/プロテオーム/トランスクリプトームのインターフェース

    佐塚隆志

    基礎生物学研究所研究会「ラン藻の分子生物学」 

    　More details

    Event date： 1999.12

    Language：Japanese   Presentation type：Oral presentation (invited, special)  

    Country：Japan  

    ", 岡崎"

  153. ラン藻プロテオームとトランスクリプトーム

    佐塚隆志

    第72回日本生化学会大会シンポジウム 

    　More details

    Event date： 1999.10

    Language：Japanese   Presentation type：Oral presentation (invited, special)  

    ", 横浜"

  154. Cyano2Dbase: Proteomic 2-D database of cyanobacterium Synechocystis sp. strain PCC68 International conference

    International Proteome & Proteomics Conference (IPPC '99) 

    　More details

    Event date： 1999.8

    Language：Japanese   Presentation type：Oral presentation (invited, special)  

    Country：Japan  

    Kisarazu Japan

  155. Interface of genome and proteome. International conference

    Meeting of the International Council of Electrophoresis Societies (Electrophoresis '99) 

    　More details

    Event date： 1999.5

    Language：English   Presentation type：Oral presentation (invited, special)  

    Country：Japan  

    Tokyo Japan

  156. ゲノム解析とプロテオーム

    佐塚隆志

    横浜市立大学木原研究所セミナー 

    　More details

    Event date： 1999.3

    Language：Japanese   Presentation type：Oral presentation (invited, special)  

    Country：Japan  

    ", 横浜"

  157. ラン藻全ゲノム配列解析からProteomicsへ

    佐塚隆志

    信州大学遺伝子実験施設第6回講演会 

    　More details

    Event date： 1999.1

    Language：Japanese   Presentation type：Oral presentation (invited, special)  

    Country：Japan  

    ", 上田"

  158. "A gene-protein linkage map of Synechocystis sp. strain PCC6803: N-terminal sequencing of 224 protein spots, subcellular localization of gene products, and thylakoidal protein specific post-translational modifications." International conference

    "XIIth International Conference on Methods in Protein Structure Analysis," 

    　More details

    Event date： 1998.9

    Language：Japanese   Presentation type：Oral presentation (general)  

    Halkidiki Greece

  159. "A proteomic study of cyanobacterium Synechocystis sp. strain PCC6803: Analysis of N-terminal sequences of the constituents of 224 protein spots resolved by 2D gel electrophoresis, construction of a 2D protein-gene linkage map and its use in gene knock-out experiments." International conference

    "From Genome To Proteome; 3rd Siena 2D Electrophoresis Meeting," 

    　More details

    Event date： 1998.8

    Language：English   Presentation type：Oral presentation (general)  

  160. ラン藻プロテオームについて

    佐塚隆志

    通産省製品評価センターセミナー 

    　More details

    Event date： 1998.1

    Language：Japanese   Presentation type：Oral presentation (invited, special)  

    ", 東京"

  161. Comprehensive analysis of the cyanobacterial proteins based on the whole genomic sequence; Toward the cyanobacterial proteome analysis. International conference

    International Workshop on Genome Project 1997 "The Frontier of Genome Research on Microorganisms" 

    　More details

    Event date： 1997.12

    Language：Japanese   Presentation type：Oral presentation (invited, special)  

    Tokyo Japan

  162. ラン藻ポストゲノム新時代の幕開け:ゲノムから包括的蛋白解析(プロテオーム)へ

    佐塚隆志

    マリンバイオテクノロジー研究会セミナー 

    　More details

    Event date： 1997.11

    Language：Japanese   Presentation type：Oral presentation (invited, special)  

    Country：Japan  

    ", 東京"

  163. "ポストゲノム研究のモデル生物,ラン藻Synechocystis sp. strain PCC6803:タンパク2次元電気泳動地図とN末端配列から見たゲノム情報"

    佐塚隆志

    大阪大学蛋白質研究所セミナー 

    　More details

    Event date： 1997.5

    Language：Japanese   Presentation type：Oral presentation (invited, special)  

    Country：Japan  

    ", 大阪"

  164. ラン藻プロテオームプロジェクト:ポストゲノム時代を迎えたラン藻 -ゲノム研究の課題とCyano2Dbase(ラン藻蛋白質二次元データベース)からのアプローチ-

    佐塚隆志

    (株)日本たばこ産業医薬基礎研究所セミナー 

    　More details

    Event date： 1996.12

    Language：Japanese   Presentation type：Oral presentation (invited, special)  

    Country：Japan  

    ", 横浜"

  165. ソルガムを原料とするバイオリファイナリー：糖化液からのカフェ酸発酵

    川口 秀夫、福本 妙子、Deddy Triyono Nugroho Adi、宮崎 翔子、佐塚 隆志、近藤 昭彦

    生物工学若手研究者の集い 夏のセミナー2019  2019.7.20 

    　More details

    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：高島  

  166. メタボローム解析が明らかにする低酸素濃度におけるコリネ型細菌の芳香族化合物代謝促進機構と代謝改変によるソルガム搾汁液からの物質生産

    川口秀夫、宮崎翔子、蓮沼誠久、荻野千秋、佐塚隆志、近藤昭彦

    日本農芸化学会2020福岡大会  2020.3.27 

    　More details

    Language：Japanese   Presentation type：Oral presentation (general)  

  167. ソルガムバガスの酵素糖化効率支配因子の探索 International conference

    川口 秀夫、佐々木 大介、福本 妙子、森田 健太、竹中 武蔵、冨田 康平、佐塚隆志、松本 拓也、西野孝、近藤 昭彦

    日本農芸化学会関西・中部支部2019年度合同神戸大会  2019.9.22 

    　More details

    Language：Japanese   Presentation type：Oral presentation (general)  

  168. セルロース系バイオマスを原料とするカフェ酸発酵のためのゲノム編集による大腸菌代謝改変の作製と培養法の検討

    第71回日本生物工学会大会  2019.9.17 

    　More details

    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：岡山  

  169. Breeding New Crops Contributing for Carbon Neutrality

    Hashimoto Shumpei, Sazuka Takashi

    Journal of Crop Research  2022  The Society of Crop Science and Breeding in Kinki, Japan

    　More details

    Language：Japanese  

    Biomass utilization can significantly contribute to carbon neutrality as biomass can be used as feedstock for petrochemical products and to generate bioenergy. In this review, we introduce sorghum, which has attracted much attention among biomass crops in recent years. In addition, we propose a rapid and effective crop breeding method based on hybrid vigor.

    DOI： 10.18964/jcr.67.0_89

    CiNii Research

  170. Analysis of photosynthetic characteristics by hierarchy in <i>sorghum</i> populations

    Ozone Gen, Oi Takao, Kono Yoshiki, Nishiuchi Shunsaku, Sazuka Takashi, Sugiura Daisuke, Taniguchi Mitsutaka

    Abstracts of Meeting of the CSSJ  2023.3.27  CROP SCIENCE SOCIETY OF JAPAN

    　More details

  171. Crop improvement and evaluation towards the realization of DAC agriculture

    Yano Masahiro, Yonemaru Jun-ichi, Sazuka Takashi, Komatsu Akira, Kinoshita Toshinori, Yoshikawa Naoki

    Breeding Research  2024.6.1  Japanese Society of Breeding

    　More details

    Language：Japanese  

    DOI： 10.1270/jsbbr.26.w01

    CiNii Research

▼display all

To the head of Presentations.▲

Research Project for Joint Research, Competitive Funding, etc. 17

  1. 雑種強勢の原理解明とその応用

    2022.6 - 2027.3

    JST  JST未来社会創造事業探索加速型（本格研究） 

    　 More details

    Authorship：Principal investigator  Grant type：Competitive

  2. 炭素超循環社会構築のための DAC 農業の実現

    2022.12 - 2025.3

    NEDO  NEDOムーンショット型研究開発事業 

    矢野昌裕

    　 More details

    Authorship：Coinvestigator(s)  Grant type：Competitive

  3. ソルガムのバイオ燃料化に関する研究

    2023.5 - 2024.3

    共同研究 

    佐塚隆志

    　 More details

    Authorship：Principal investigator  Grant type：Collaborative (industry/university)

  4. 光スイッチ型海洋分解性の可食プラスチックの開発研究

    2020.11 - 2023.3

    NEDO  NEDOムーンショット型研究開発事業 

    金子達夫

    　 More details

    Authorship：Coinvestigator(s)  Grant type：Competitive

  5. 基礎植物科学から作物育種への応用展開

    2018.4 - 2022.3

    科学技術ハブ 

    　 More details

    Authorship：Coinvestigator(s)  Grant type：Other

  6. 雑種強勢の原理解明とその応用

    2017.11 - 2021.5

    JST未来社会創造事業探索加速型（探索研究） 

    佐塚隆志

  7. ソルガム穂の形態形成遺伝子の単離と機能解明

    2013 - 2015

    農水省・ゲノム情報を活用した農畜産物の次世代生産基盤技術の開発プロジェクト 

    　 More details

    Authorship：Principal investigator 

  8. ソルガム突然変異集団の作出とスクリーニング系の構築

    2013 - 2015

    農水省・ゲノム情報を活用した農畜産物の次世代生産基盤技術の開発プロジェクト 

    　 More details

    Authorship：Principal investigator  Grant type：Collaborative (industry/university)

  9. CO2削減及び資源化に最適化した高バイオマス作物の育種開発

    2011 - 2016.3

    植物CO2資源化研究拠点ネットワーク(NC-CARP)  

    　 More details

    Authorship：Coinvestigator(s) 

  10. 有用突然変異体の誘起とその同定

    2008 - 2013

    農水省・新農業展開プロジェクト 

    　 More details

    Authorship：Principal investigator  Grant type：Competitive

  11. ソルガムの有用突然変異体の誘起とその同定

    2008

    農水省・新農業展開プロジェクト  

    　 More details

    Authorship：Principal investigator  Grant type：Competitive

  12. ソルガムの節間伸長および高糖性に関与する遺伝子の解析

    2008

    　 More details

    Authorship：Principal investigator  Grant type：Competitive

  13. イネにおける胚発生突然変異体の研究

    2004

    　 More details

    Authorship：Principal investigator  Grant type：Competitive

  14. バイオリファイナリー産業を加速化するソルガム高糖性遺伝子の同定

    0215.4 - 2016.3

    科学研究費挑戦的萌芽研究 

    　 More details

    Authorship：Principal investigator  Grant type：Competitive

  15. ソルガムのバイオマス及び子実利用を目指した遺伝育種学的研究

    国内共同研究 

  16. ルガムのバイオマス及び糖質利用における育種素材のスクリーニングと適性評価

    国内共同研究 

  17. 雑種強勢の原理解明とその応用

    JST未来社会創造事業 

▼display all

To the head of Research Project for Joint Research, Competitive Funding, etc..▲

KAKENHI (Grants-in-Aid for Scientific Research) 5

  1. Searching for genes responsible for adapting to environmental change using next-generation GWAS

    Grant number：22H02294  2022.4 - 2026.3

    Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

    　 More details

    Authorship：Coinvestigator(s)  Grant type：Competitive

    Grant amount：\1500000 （ Direct Cost: \1500000 ）

  2. Identification of sorghum genes controlling brix to accelerate the biorefinery use

    Grant number：15K14624  2015.4 - 2016.3

    Grants-in-Aid for Scientific Research  Grant-in-Aid for Challenging Exploratory Research

    Sazuka Takashi

    　 More details

    Authorship：Principal investigator 

    Grant amount：\3900000 （ Direct Cost: \3000000 、 Indirect Cost：\900000 ）

    In biorefinery, sugar is a key molecule, and genes controlling brix is the key genes. In our previous studies, the introgression of a QTL associated brix (qBRX-6) detected in sweet sorghum (SIL-05) increased the brix score in culm. However, Ma1/SbPRR37 disturbed the marker assisted selection in breeding, because it located nearby qBRX-6. In this study, we narrowed down the candidate region of qBRX-6 into about 190kb using a chromosome segment substitution line. Based on this results, we established a DNA marker that can distinguish two loci.

  3. Genetic analysis of seeds for high sugar content suitable for production of bioethanol and high quality silage

    Grant number：23658007  2011 - 2012

    Grants-in-Aid for Scientific Research  Grant-in-Aid for Challenging Exploratory Research

    SAZUKA Takashi, KASUGA Shigemitsu

    　 More details

    Authorship：Principal investigator 

    Grant amount：\3770000 （ Direct Cost: \2900000 、 Indirect Cost：\870000 ）

    Sugary feterita is a cultivar of Sorghum bicolor (L.) Moench, which accumulates sugar in the seeds. Here, we generated an F2 population by crossing Sugary Feterita with Nakei MS-3B which accumulates sugar in the seeds at low levels. An analysis of the segregation ratio of the F2 population indicated that the phenotype is determined by a recessive gene. A rough mapping analysis indicated that the corresponding gene is located on chromosome 7. Within the candidate region, a gene that encodes a starch biosynthetic enzyme, SbSU1, was located. This gene is an ortholog of maize SU1, the mutation of which is responsible for the high sugar content in the seeds of sweet corn. We carried out sequence analysis of the gene from both parental lines and found someSNPs. Two of them result in amino acid substitutions. After comparing SbSU1 with other orthologous genes from other species, we confirmed that Sugary Feterita possesses a mutant allele. SbSU1 transcripts increased toward flowering time, during which starch is being accumulated in wild-type (Feterita) seeds. These results suggest that the mutation(s) in SbSU1 of Sugary Feterita might also cause abnormal starch synthesis, consequently resulting in the accumulation of sugars in the seeds.

  4. Functional Analysis of auxin and microRNA in organ formation during rice embryogenesis

    Grant number：20570034  2008 - 2010

    Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    SAZUKA Takashi, KITANO Hidemi

    　 More details

    Authorship：Principal investigator 

    Grant amount：\4810000 （ Direct Cost: \3700000 、 Indirect Cost：\1110000 ）

    The three rice mutants, club-shaped embryo1 (cle1), club-shaped embryo3 (cle3), tryptphan deficient dwarf1 (tdd1) were used to study the regulation for organ differentiation in rice embryogenesis by auxin and miRNA. The embryonic phenotype of two mutants, cle1 and cle3, are club-shaped, and that of tdd1 is globular ; the three mutants are defective for the organ formation during embryogenesis. The positional cloning indicates that the responsible genes for cle1 and tdd1 are OSDCL1 and ASB1, respectively. The detailed analysis revealed the important regulation by auxin and miRNA for rice embryogenesis. The other two mutants, cre3 and tdd1, were also analyzed.

  5. Biological analysis on decision mechanism of the ideal plant type for hyper-yield rice breeding

    Grant number：18380004  2006 - 2008

    Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

    KITANO Hidemi, SAZUKA Takashi

    　 More details

    Authorship：Coinvestigator(s)  Grant type：Competitive

To the head of KAKENHI (Grants-in-Aid for Scientific Research).▲

Industrial property rights 1

  1. 74LH改0号（品種登録）

    佐塚隆志、春日重光

    　More details

    Applicant：国立大学法人名古屋大学

    Application no：第30854号  Date applied：2016.2

    Date announced：2024.9

    Date published：2024.9

    Patent/Registration no：29901  Date registered：2024.9  Date issued：2024.9

    Rights holder：佐塚隆志、春日重光（育成者）   Country of applicant：Domestic  

To the head of Industrial property rights.▲
 

Teaching Experience (On-campus) 30

  1. 植物育種学

    2023

  2. 修士論文研究1

    2023

  3. 生命農学演習4

    2023

  4. 生命農学演習3

    2023

  5. 生命農学演習2

    2023

  6. 生命農学演習1

    2023

  7. 資源生物学科実験実習

    2023

  8. 細胞工学

    2023

  9. 修士論文研究2

    2023

  10. 修士論文研究4

    2023

  11. 修士論文研究3

    2023

  12. 資源生物学科実験実習

    2022

  13. 生命農学演習1

    2022

  14. 生命農学演習2

    2022

  15. 生命農学演習3

    2022

  16. 生命農学演習4

    2022

  17. 修士論文研究1

    2022

  18. 修士論文研究2

    2022

  19. 修士論文研究3

    2022

  20. 修士論文研究4

    2022

  21. Biotechnology

    2020

  22. 修士論文研究４

    2020

  23. 修士論文研究３

    2020

  24. 修士論文研究２

    2020

  25. 修士論文研究１

    2020

  26. 生命農学演習３

    2020

  27. 生命農学演習２

    2020

  28. 生命農学演習１

    2020

  29. 植物分子育種学特論

    2020

  30. 資源生物科学実験実習1

    2020

▼display all

To the head of Teaching Experience (On-campus).▲

Teaching Experience (Off-campus) 2

  1. 2002.4 - 2003.3 （東京大学大学院医学系研究科）

  2. 2000.4 - 2001.3 （茨城大学農学部）

To the head of Teaching Experience (Off-campus).▲
 

Social Contribution 3

  1. 藤森中学校講演

    Role(s)：Lecturer

    名古屋市立藤森中学校  2024.1

  2. 近畿作物育種研究会シンポジウム

    Role(s)：Panelist

    近畿作物育種研究会  2021.12

  3. 岡山大学資源植物科学研究所シンポジウム

    Role(s)：Panelist

    岡山大学資源植物科学研究所  2021.3

To the head of Social Contribution.▲

Media Coverage 10

  1. 産官学　5者連携！ Newspaper, magazine

    愛知県酪農クラスター協議会  クラスター通信（広報誌）  産官学　5者連携！  2023.8

  2. 植物ソルガム「一挙三得」車部品・飼料・燃料に活用 Newspaper, magazine

    日経産業新聞  2023.8

    　More details

    Author：Other 

  3. 「脱炭素」☓「農業」に挑戦 Newspaper, magazine

    中部経済新聞  中部経済新聞  2023.2

    　More details

    Author：Other 

  4. ソルガム栽培の普及で脱炭素社会へ Promotional material

    豊田市農業委員会  豊田市農業委員会だより  第21号, p6-p7  2022.11

    　More details

    Author：Other 

  5. 栽培から発電燃料化までの地域資源の循環モデルを目指す植物「ソルガム」の研究開発 Promotional material

    中部電力  交流  No. 125, p17-p19  2022.9

    　More details

    Author：Other 

  6. ソルガムのカスケード利用「有価物と発電燃料生産へ」 Newspaper, magazine

    電気新聞  時代の要請に応え挑戦  2022.4

    　More details

    Author：Other 

  7. 植物由来繊維を車部品に Newspaper, magazine

    日経産業新聞  2021.12

  8. 植物由来繊維　車部品に Newspaper, magazine

    日本経済新聞  2021.12

    　More details

    Author：Other 

  9. 日経深読み！ TV or radio program

    愛知テレビ  もっと5時スタ　  2021.12

    　More details

    Author：Other 

  10. 豊田鉄工・名古屋大 植物”ソルガム”で車部品を TV or radio program

    テレビ愛知  もっと5時スタ  0221.12

    　More details

    Author：Other 

▼display all

To the head of Media Coverage.▲

Academic Activities 2

  1. Sorghum workshop International contribution

    Role(s)：Planning, management, etc.,　Panel moderator, session chair, etc.

    2023.11 - 2023.12

    　More details

    Type：Academic society, research group, etc. 

  2. JSTキックオフイベントシンポジウム

    Role(s)：Planning, management, etc.,　Panel moderator, session chair, etc.

    名古屋大学生物機能開発利用研究センター　他  2021.12

    　More details

    Type：Competition, symposium, etc. 

To the head of Academic Activities.▲