Updated on 2022/04/21

写真a

 
NAKANISHI, Yoichi
 
Organization
Graduate School of Bioagricultural Sciences Department of Applied Biosciences Assistant Professor
Graduate School
Graduate School of Bioagricultural Sciences
Undergraduate School
School of Agricultural Sciences
Title
Assistant Professor

Degree 1

  1. 博士(農学) ( 2000.3   名古屋大学 ) 

Research Interests 6

  1. plant

  2. transporter

  3. vacuole

  4. FRET sensor

  5. molybdenum

  6. cyanobacteria

Research Areas 1

  1. Others / Others  / Applied Biochemistry

Current Research Project and SDGs 3

  1. 真核微生物膜輸送体の機能解析

  2. 植物液胞膜輸送体の遺伝子同定、機能解析

  3. 翻訳システム改変によるラン藻の生産性向上

Research History 4

  1. Assistant Professor

    2007.4

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    Country:Japan

  2. Research Assistant

    2003.4 - 2007.3

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    Country:Japan

  3. Researcher, ATP System, ERATO, JST

    2002.4 - 2003.3

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    Country:Japan

  4. 日本学術振興会 特別研究員 PD

    2000.4 - 2003.3

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    Country:Japan

Education 2

  1. Nagoya University   Graduate School of Bioagricultural Sciences

    1997.4 - 2000.3

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    Country: Japan

  2. Nagoya University   Faculty of Agriculture

    - 1993.3

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    Country: Japan

Professional Memberships 4

  1. 日本植物生理学会

  2. 日本農芸化学会

  3. 日本分子生物学会

  4. 日本生化学会

 

Papers 27

  1. Exploring dynamics of molybdate in living animal cells by a genetically encoded FRET nanosensor Reviewed

    Nakanishi Y, Iida S, Ueoka-Nakanishi H, Niimi T, Tomioka R, Maeshima M.

    PLoS ONE   Vol. 8 ( 3 ) page: e58175   2013.3

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1371/journal.pone.0058175

  2. Biochemical, structural, and physiological characteristics of vacuolar H+-pyrophosphatase.

    Segami S, Asaoka M, Kinoshita S, Fukuda M, Nakanishi Y, Maeshima M

    Plant & cell physiology   Vol. 59 ( 7 ) page: 1300 - 1308   2018.7

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    Language:Japanese   Publishing type:Research paper (scientific journal)  

    DOI: 10.1093/pcp/pcy054

    Web of Science

    PubMed

  3. FRET-based detection of isozyme-specific activities of transglutaminases

    Tatsukawa Hideki, Liu Hong Hong, Oba Shota, Kamiya Noriho, Nakanishi Yoichi, Hitomi Kiyotaka

    AMINO ACIDS   Vol. 49 ( 3 ) page: 615-623   2017.3

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1007/s00726-016-2322-0

    Web of Science

    PubMed

  4. Studies on vacuolar membrane microdomains isolated from Arabidopsis suspension-cultured cells: local distribution of vacuolar membrane proteins. Reviewed

    Yoshida K, Ohnishi M, Fukao Y, Okazaki Y, Fujiwara M, Song C, Nakanishi Y, Saito K, Shimmen T, Suzaki T, Hayashi F, Fukaki H, Maeshima M, Mimura T.

    Plant Cell Physiol.   Vol. 54 ( 10 ) page: 1571-1584   2013.7

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1093/pcp/pct107.

  5. Thioredoxin h regulates calcium dependent protein kinases in plasma membranes. Reviewed

    Ueoka-Nakanishi H, Sazuka T, Nakanishi Y, Maeshima M, Mori H, Hisabori T.

    FEBS J.   Vol. 280 ( 14 ) page: 3220-3231   2013.6

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1111/febs.12301.

  6. Quantification, organ-specific accumulation and intracellular localization of type II H(+)-pyrophosphatase in Arabidopsis thaliana. Reviewed

    Plant Cell Physiol.   Vol. 51 ( 8 ) page: 1350-1360   2010.8

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    Most plants have two types of H(+)-translocating inorganic pyrophosphatases (H(+)-PPases), I and II, which differ in primary sequence and K(+) dependence of enzyme function. Arabidopsis thaliana has three genes for H(+)-PPases: one for type I and two for type II. The type I H(+)-PPase requires K(+) for maximal enzyme activity and functions together with H(+)-ATPase in vacuolar membranes. The physiological role of the type II enzyme, which does not require K(+), is not clear. We focused on the type II enzymes (AtVHP2;1 and AtVHP2;2) of A. thaliana. Total amounts of AtVHP2s were quantified immunochemically using a specific antibody and determined to be 22 and 12 ng mg(-1) of total protein in the microsomal fractions of suspension-cultured cells and young roots, respectively, and the values are approximately 0.1 and 0.2%, respectively, of the vacuolar H(+)-PPase. In plants, AtVHP2s were detected immunochemically in all tissues except mature leaves, and were abundant in roots and flowers. The intracellular localization of AtVHP2s in suspension cells was determined by sucrose density gradient centrifugation and immunoblotting. Comparison with a number of marker proteins revealed localization in the Golgi apparatus and the trans-Golgi network. These results suggest that the type II H(+)-PPase functions as a proton pump in the Golgi and related vesicles in young tissues, although its content is very low compared with the type I enzyme.

  7. Characterization of a tobacco TPK-type K+ channel as a novel tonoplast K+ channel using yeast tonoplasts. Reviewed

    Hamamoto S, Marui J, Matsuoka K, Higashi K, Igarashi K, Nakagawa T, Kuroda T, Mori Y, Murata Y, Nakanishi Y, Maeshima M, Yabe I, Uozumi N.

    J Biol Chem.   Vol. 283 ( 4 ) page: 1911-1920   2008.1

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  8. Identification of amino acid residues participating in the energy coupling and proton transport of Streptomyces coelicolor A3(2) H+-pyrophosphatase. Reviewed

    Hirono M, Nakanishi Y, Maeshima M.

    Biochim Biophys Acta.   Vol. 1767   page: 1401-11   2007.12

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  9. Essential amino acid residues in the central transmembrane domains and loops for energy coupling of Streptomyces coelicolor A3(2) H+-pyrophosphatase. Reviewed

    Hirono M, Nakanishi Y, Maeshima M

    Biochimica et Biophysica Acta   Vol. 1767 ( 7 ) page: 930-939   2007.7

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    Language:English   Publishing type:Research paper (scientific journal)  

  10. プロトン輸送性ピロホスファターゼ:低コスト化合物を利用するポンプ (シンプルなプロトンポンプの機能を支える分子構造)

    広野めぐみ,三村久敏,瀬上紹嗣,中西洋一,前島正義

    生化学   Vol. 79 ( 6 ) page: 535-541   2007.6

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    Language:Japanese  

  11. Expression of functional Streptomyces coelicolor H+-pyrophosphatase and characterization of its molecular properties. Reviewed

    Hirono M, Mimura H, Nakanishi Y, Maeshima M

    Journal of Biochemistry (Tokyo)   Vol. 138 ( 2 ) page: 183-191   2005.8

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  12. Disulfide-bond formation in the H+-pyrophosphatase of Streptomyces coelicolor and its implications for redox control and enzyme structure. Reviewed

    Mimura H, Nakanishi Y, Maeshima M.

    FEBS Letter   Vol. 579 ( 17 ) page: 3625-3631   2005.7

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    Language:English   Publishing type:Research paper (scientific journal)  

  13. Oligomerization of H(+)-pyrophosphatase and its structural and functional consequences. Reviewed

    Mimura H, Nakanishi Y, Maeshima M

    Biochimica et Biophysica Acta   Vol. 1708 ( 3 ) page: 393-403   2005.7

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  14. Membrane topology of the H+-pyrophosphatase of Streptomyces coelicolor determined by cysteine-scanning mutagenesis. Reviewed

    Mimura H, Nakanishi Y, Hirono M, Maeshima M

    The Journal of Biological Chemistry   Vol. 279 ( 33 ) page: 35106-35112   2004.8

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    Language:English   Publishing type:Research paper (scientific journal)  

  15. Inverse regulation of rotation of F1-ATPase by the mutation at the regulatory region on the gamma subunit of chloroplast ATP synthase.

    Ueoka-Nakanishi, H., Nakanishi, Y., Konno, H., Motohashi, K., Bald, D. and Hisabori, T.

    The Journal of Biological Chemistry   Vol. 279 ( 16 ) page: 16272-16277   2004.4

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    Language:English   Publishing type:Research paper (scientific journal)  

  16. *Patch clamp analysis of a H+ pump heterologously expressed in giant yeast vacuoles. Reviewed

    Nakanishi Y, Yabe I, Maeshima M

    Journal of Biochemistry (Tokyo)   Vol. 134 ( 4 ) page: 615-623   2003.10

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

  17. Chloroplast cyclophilin is a target protein of thioredoxin. Thiol modulation of the peptidyl-prolyl cis-trans isomerase activity. Reviewed

    Motohashi K, Koyama F, Nakanishi Y, Ueoka-Nakanishi H, Hisabori T.

    J Biol Chem.   Vol. 278 ( 34 ) page: 31848-31852   2003.8

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  18. Acylspermidine derivatives isolated from a soft coral, Sinularia sp, inhibit plant vacuolar H(+)-pyrophosphatase. Reviewed

    Hirono M, Ojika M, Mimura H, Nakanishi Y, Maeshima M

    Journal of Biochemistry (Tokyo)   Vol. 133 ( 6 ) page: 811-816   2003.6

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  19. Functional expression of Acetabularia acetabulum vacuolar H(+)-pyrophosphatase in a yeast VMA3-deficient strain. Reviewed

    Ikeda M, Umami K, Hinohara M, Tanimura Y, Ohmae A, Nakanishi Y, Maeshima M.

    J Exp Bot.   Vol. 53 ( 378 ) page: 2273-2275   2002.11

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  20. [Calcium accumulation system and proton pumps in plant vacuoles].

    Nakanishi Y, Ueoka-Nakanishi H, Yuasa K, Mimura H, Maeshima M.

      Vol. 47 ( 12 ) page: 1777-82   2002.9

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    Language:Japanese  

  21. Expression of V-ATPase proteolipid subunit of Acetabularia acetabulum in a VMA3-deficient strain of Saccharomyces cerevisiae and its complementation study. Reviewed

    Ikeda M, Hinohara M, Umami K, Taguro Y, Okada Y, Wada Y, Nakanishi Y, Maeshima M.

    Eur J Biochem.   Vol. 268 ( 23 ) page: 6097-6104   2001.12

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  22. *Mutagenic analysis of functional residues in putative substrate-binding site and acidic domains of vacuolar H+-pyrophosphatase. Reviewed

    Nakanishi, Y., Saijo, T., Wada, Y. and Maeshima, M.

    The Journal of Biological Chemistry   Vol. 276 ( 10 ) page: 7654-7660   2001.3

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  23. Functional expression of mung bean Ca2+/H+ antiporter in yeast and its intracellular localization in the hypocotyl and tobacco cells. Reviewed

    Ueoka-Nakanishi H, Tsuchiya T, Sasaki M, Nakanishi Y, Cunningham KW, Maeshima M.

    Eur J Biochem.   Vol. 267 ( 10 ) page: 3090-3098   2000.5

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  24. Properties and molecular cloning of Ca2+/H+ antiporter in the vacuolar membrane of mung bean. Reviewed

    Ueoka-Nakanishi H, Nakanishi Y, Tanaka Y, Maeshima M.

    Eur J Biochem.   Vol. 262 ( 2 ) page: 417-425   1999.6

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  25. *Molecular cloning and sequencing of the cDNA for vacuolar H+-pyrophosphatase from Chara corallina. Reviewed

    Nakanishi Y, Matsuda N, Aizawa K, Kashiyama T, Yamamoto K, Mimura T, Ikeda M,Maeshima M

    Biochim Biophys Acta   Vol. 1418 ( 1 ) page: 245-250   1999.4

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  26. *Molecular cloning of vacuolar H(+)-pyrophosphatase and its developmental expression in growing hypocotyl of mung bean. Invited Reviewed

    Nakanishi Y, Maeshima M

    Plant Physiolgy   Vol. 116 ( 2 ) page: 589-597   1998.2

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

  27. Analysis of the substrate binding site and carboxyl terminal region of vacuolar H+-pyrophosphatase of mung bean with peptide antibodies.

    Takasu A, Nakanishi Y, Yamauchi T, Maeshima M.

    J Biochem.   Vol. 122 ( 4 ) page: 883-889   1997.10

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Books 1

  1. 新しいイオン輸送体アッセイ法:E-cellのデータベースの構築を目指す「リポソーム応用の新展開~人工細胞への開発に向けて」

    黒田照夫、中西洋一、岩崎郁子、矢部勇( Role: Joint author)

    NTS出版  2005 

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    Language:Japanese

Presentations 1

  1. 液泡型H+-ATPaseの活性調節とその仕組み

    中西洋一、前島正義、久堀徹

    「第7回植物生体膜シンポジウム:生体膜研究の新世紀」 

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    Event date: 2004.3

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

KAKENHI (Grants-in-Aid for Scientific Research) 6

  1. ラン藻の翻訳システム改変と大規模な外来遺伝子導入の研究

    Grant number:21K19217  2021.7 - 2024.3

    科学研究費助成事業  挑戦的研究(萌芽)

    中西 洋一

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    Authorship:Principal investigator 

    Grant amount:\6370000 ( Direct Cost: \4900000 、 Indirect Cost:\1470000 )

    シアノバクテリア(ラン藻)は一般生物でイソロイシンをコードするRNAコドン”AUA”を認識するtRNAそのものをもたないため、外来遺伝子を利用した基礎研究・応用研究が困難である。本研究では、ラン藻に他の生物のtRNAIle2システムをまるごと導入して“AUA”コドンを利用できるようにする。さらに、改変株に高等植物由来の遺伝子を大規模導入して革新的な機能性ラン藻を作出するモデル研究を行う。

  2. 機能株群集成長解析を活用した植物有害物質膜輸送体の包括的機能ゲノム解析

    2012.4 - 2014.3

    科学研究費補助金 

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    Authorship:Principal investigator 

  3. ハンセヌラ酵母をモデルにした真核生物モリブデン膜輸送システムの分子遺伝学的研究

    2010.4 - 2012.3

    科学研究費補助金  若手研究(B),課題番号:22780089

    中西 洋一

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  4. 膜輸送体候補遺伝子の総当たり過剰発現解析による新しい元素膜輸送の発見

    2008.11 - 2012.3

    科学研究費補助金  新学術領域研究

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    Authorship:Principal investigator 

  5. 真核生物におけるモリブデン膜輸送システムの研究

    2008.4 - 2010.3

    科学研究費補助金  若手研究(B),課題番号:20780073

    中西 洋一

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    Authorship:Principal investigator 

  6. アントシアニンの発色に関わる膜輸送体のゲノムレベルでの探索

    2006.4 - 2008.3

    科学研究費補助金  若手研究(B),課題番号:18780071

    中西 洋一

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Industrial property rights 4

  1. 核酸合成用酵素組成物及び核酸合成法

    中西洋一

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    Applicant:国立大学法人名古屋大学

    Application no:2010-152448  Date applied:2010.7

    Announcement no:2012-10666 

    Country of applicant:Domestic  

    様々な夾雑物の存在下でも容易に目的遺伝子を鋳型とする鎖伸長反応による核酸合成を行うことができる核酸合成用酵素組成物及び該組成物を用いた核酸合成法を提供する。

  2. ピロリン酸測定方法及びプライマー伸張反応検出方法、並びにこれら方法を実施するための装置

    夜久 英信,前島 正義,中西 洋一,広野 めぐみ,行政 哲男,岡 弘章

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    Applicant:パナソニック株式会社

    Application no:PCT/JP2005/005522  Date applied:2005.3

    Announcement no:WO2005/093088 

    Country of applicant:Domestic  

  3. Method of measuring pyrophosphate and method of detecting primer extension reaction, and device for performing the same

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    Application no:2004095154  Date applied:2004.3

    Announcement no:20060211005 

    Patent/Registration no:20060211005  Date registered:2006 

    Country of applicant:Foreign country  

  4. *放線菌H+-pyrophosphatase合成遺伝子の作成と大腸菌での発現システム

    夜久英信, 前島正義, 中西洋一, 広野めぐみ, 岡弘章

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    Applicant:松下電器産業株式会社

    Application no:特許出願2004-95153  Date applied:2004.3

    Announcement no:特許公開2007-222003 

    Patent/Registration no:特許公開2007-222003  Date registered:2006 

    Country of applicant:Domestic  

 

Teaching Experience (On-campus) 2

  1. 応用生命科学科学生実習

    2016

  2. 情報リテラシー

    2016