研究者詳細 - 塚田　光

論文 - 塚田　光

分割表示 44 件中 21 - 40 件目／全件表示 >>

Primary structure, tissue distribution, and biological activity of chicken motilin receptor. 査読有り

Ichiro Yamamoto, Hiroyuki Kaiya, Chihiro Tsutsui, Takafumi Sakai, Akira Tsukada, Mikiya Miyazato, Minoru Tanaka

General and Comparative Endocrinology 156 巻頁： 509-514 2008年

Z-chromosome specific primers for chicken-quail hybrid blastoderm. 査読有り

44 巻 ( 2 ) 頁： 209-212 2007年4月

Fertilization and blastoderm development of quail oocytes after intracytoplasmic injection of chicken sperm bearing a W chromosome. 査読有り

86 巻 ( 4 ) 頁： 731-738 2007年

Fertilizing ability of chicken sperm bearing W chromosome. 査読有り

Takagi S., Tsukada A., Saito N. and Shimada K.

Poultry Science 86 巻 ( 5 ) 頁： 937-943 2007年

記述言語：英語掲載種別：研究論文（学術雑誌）

In the sex-reversed domestic fowl, spermatids and sperm carrying the female-specific W chromosome have been demonstrated, but whether the spermatids can become functional sperm and can fertilize the oocyte remains undetermined. In the present study, sex reversal was induced by injection of a nonsteroidal aromatase inhibitor (Fadrozole) into the air sac of the chicken egg on d 4 of incubation, and the chicks were reared to 18 mo old. A single elongated spermatid or sperm was isolated from the testis from either normal roosters or sex-reversed hens, and each was microinjected into a mouse oocyte and cultured for 24 h. Although injected oocytes were monitored on the stage of microscope, they were classified into groups by the number of pronuclei. Those that showed male and female pronuclei (2PN) were considered to have oocyte-activating potency. In the normal rooster group, most semen and testicular sperm induced 2PN, whereas only half of the elongated spermatids induced 2PN. In the sex-reversal group, most testicular sperm induced 2PN, whereas nearly half of the elongated spermatids induced 2PN in the oocytes. There was no pronucleus in the oocytes after microinjection of medium only. A second experiment confirmed the higher rate of oocyte activation by testicular sperm than testicular elongated spermatids. In this second experiment, individual oocytes injected with spermatids and sperm of sex-reversed hens were assayed by PCR to identify the W chromosome. Most spermatids and sperm carried Z chromosome, whereas a minority carried W chromosome. However, the sperm carrying W chromosome evoked 2PN with the same rate of oocyte activation as those carrying Z chromosome. From these results, it is concluded that the chicken elongated spermatids and sperm carrying W chromosome may possess a fertilizing ability similar to normal chicken sperm carrying the Z chromosome.

Primary Structure And Tissue Distribution Of GPR39 mRNA In Japanese Quail,Coturnix japonica. 査読有り

I. Yamamoto, Y. Sakaguchi, M. Numao, A. Tsukada, N. Tsushima and M. Tanaka

Poultly Sience 2007 巻 ( 86 ) 頁： 2472-2476 2007年

Possible Role of Calcium on Oocyte Development After Intracytoplasmic Sperm Injection in Quail (Coturnix japonica) 査読有り

SHUSEI MIZUSHIMA, SOICHI TAKAGI, TAMAO ONO, YUSUKE ATSUMI, AKIRA TSUKADA, NOBORU SAITO, AND KIYOSHI SHIMADA

JOURNAL OF EXPERIMENTAL ZOOLOGY 307A 巻頁： 1-7 2007年

Plasma Prolactin and Corticosterone Concentrations Are Changing Toward hatch with a Different Manner Between Layer- and Broiler- Type Chickens. 査読有り

M. Sato, T. Tachibana, A. Tsukada and M. Furuse.

Journal of Animal and Veterinary Advances 6 巻 ( 8 ) 頁： 923-926 2007年

Identification of spermatogenic cells expressing protamine mRNA in Japanese quail by RT-PCR.

"Upi Chairun Nisa, Akira Tsukada, Soichi Takagi, Ichiro Yamamoto, Yonju Ha, Anna Hrabia, Noboru Saito and Kiyoshi Shimada."

Journal of Poultry Science 42(1) 巻頁： 70-78. 2005年1月

Comparison of mammalian prolactin-releasing peptide and Carassius RFamide for feeding behavior and prolactin secretion in chicks. 査読有り

Tetsuya Tachibana, Akira Tsukada, Masaaki Fujimoto, Hirokazu , Takahashi, Takeshi Ohkubo, Tim Boswell, Mitsuhiro Furuse.

General and Comparative Endocrinology 144 巻頁： 264-269 2005年

Changes in mRNA expression of MMP-2 in the M殕lerian duct of chicken Embryo.

"Ha, Y., Tsukada,A., Sato, N. and Shimada, K."

Gen Comp Endocrinol. 139(2) 巻頁： 131-136. 2004年12月

Photoinducible phase specific light induction of Cry1 gene in the pars tuberalis of Japanese quail

"Yasuo S, Watanabe M, Tsukada A, Takagi T, Iigo M, Shimada K, Ebihara S, Yoshimura T"

Endocrinology 145 巻頁： 1612-1616 2004年4月

Effect of central administration of prolactin-releasing peptide on feeding in chicks

"Tetsuya Tachibana, Shin Saito, Shozo Tomonaga, Tomo Takagi, Ei-Suke Saito, Tomonori Nakanishi, Tomoyuki Koutoku, Akira Tsukada, Takeshi Ohkubo, Tim Boswell and Mitsuhiro Furuse"

Physiology & Behavior 80 巻頁： 713-719 2004年1月

cDNA cloning of chicken orexin receptor and tissue distribution: sex dimorphic expression in chicken gonads

"Takeshi Ohkubo, Akira Tsukada and K Shamoto"

Journal of Molecular Endocrinology 31 巻頁： 499-508 2003年11月

cDNA cloning and mRNA Expression of estrogen receptor alpha in the Japanese quail.

"Kouhei Ichikawa, Ichiro Yamamoto, Akira Tsukada, Noboru saito and Kiyoshi Shhimada"

Journal Poultry Science 40 巻頁： 121-129 2003年4月

Effect of Endocrine Disrupters on mRNA Expression of Vitellogenin (VTG) II and Very Low Density Lipoprotein (apoVLDL) II in the Liver of Quail Embryos.

"Kouhei Ichikawa, Yonju Ha, Akira Tsukada, Noboru saito and Kiyoshi Shhimada"

Journal Poultry Science 40 巻頁： 45-52 2003年2月

Profiles of mRNA expression of genes related to sex differentiation of the gonads in the chicken embryo

"Ichiro Yamamoto, Akira Tsukada, Noboru Saito and Kiyoshi Simada"

Poult ry Science 82 巻頁： 1462-1467 2003年1月

Expression of protamine mRNA in relation to spermatogenic activity in Japanese quail

"Upi Chairun Nisa, Akira Tsukada, Masahiko Mori, Noboru Saito and Kiyoshi Shimada"

Journal of Poultry Science 40 巻頁： 309-318 2003年1月

"Effect of Estradiol and Nonylphenol on mRNA Expression of Estrogen Receptors α and β, and Cytochrome P450 Aromatase in the Gonad of Chicken Embryo."

"Masanori Sakimura, Akira Tsukada, Makoto Usami, Shozo Hanzawa, Noboru Saito, Yasuo Ohno and Kiyoshi Shimada."

Journal Poultry Science 39 巻頁： 302-309 2002年10月

cDNA cloning and mRNA expression Transformer 2.(Tra2) in chicken embryo.

"I. Yamamoto, A. Tsukada, N. Saito, K. Simada"

Biochimica et Biophysica Acta 1579 巻頁： 185-188 2002年9月

Suppressive effect of p-nonylphenol on male-specific mRNA expression in the embryonic gonad of chickens.

"Sakimura M, Usami M, Hanzawa S, Tsukada A, Saito N, Yasuo Ohno and Shimada K."

Journal of poultry science 39 巻頁： 91-99 2002年4月

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