Updated on 2022/04/26

写真a

 
TSUKADA, Akira
 
Organization
Graduate School of Bioagricultural Sciences Assistant Professor
Graduate School
Graduate School of Bioagricultural Sciences
Undergraduate School
School of Agricultural Sciences
Title
Assistant Professor

Degree 2

  1. 修士 ( 1996.3   三重大学 ) 

  2. 博士(学術) ( 1999.3   三重大学 ) 

Research Interests 4

  1. growth

  2. Growth Hormone

  3. chicken

  4. body size

Research Areas 1

  1. Life Science / Animal production science  / Applied Animal Science

Current Research Project and SDGs 1

  1. "受精により生まれた細胞は様々な分化の過程を経,多様な細胞をもった成熟個体となる.その中でも生殖細胞は個体の発生の初期から次世代へと受け継がれる細胞であることが知られている.鳥類をもちい,特に生殖細胞,肝細胞の分化・増殖機構の解明を目指している."

Research History 3

  1. Nagoya University   Assistant Professor

    2007.4

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    Country:Japan

  2. Nagoya University   Assistant Professor

    2001.11 - 2007.3

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    Country:Japan

  3. Japan Society for Promotion of Science   Researcher

    1999.4 - 2001.11

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    Country:Japan

Education 3

  1. 三重大学大学院   生物資源学   生物資源開発科学

    - 1999.3

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    Country: Japan

  2. 三重大学大学院   生物資源学   農業生産

    - 1996.3

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    Country: Japan

  3. Mie University   Faculty of Bioresources

    - 1994

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    Country: Japan

Professional Memberships 3

  1. Japan Poultry Science Association

    2006.4

  2. Japanese Society of Animal Science

  3. 東海畜産学会   県幹事

    2015.4 - 2017.3

Committee Memberships 2

  1. 10th International Symposium on Avian Endocrinology岐阜大会組織委(2011-2012)   委員  

    2011 - 2012   

  2. 畜産学会名古屋大会組織委員(2012)   委員  

    2012   

 

Papers 44

  1. Heat exposure alters the mRNA expression of growth- and stress-related genes in chicks

    Okuyama Hirofumi, Khan Md. Sakirul Islam, Tsukada Akira, Tachibana Tetsuya

    LIVESTOCK SCIENCE   Vol. 198   page: 97-103   2017.4

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.livsci.2017.02.010

    Web of Science

  2. Ubiquitin-Specific Peptidase 46 (Usp46) Regulates Mouse Immobile Behavior in the Tail Suspension Test through the GABAergic System Reviewed

    Saki Imai1, Takayoshi Mamiya, Akira Tsukada, Yasuyuki Sakai, Akihiro Mouri, Toshitaka Nabeshima, Shizufumi Ebihara

    PLoS ONE   Vol. 7 ( 6 ) page: e39084   2012

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1371/journal.pone.0039084

  3. Progesterone is a sperm releasing factor from the sperm storage tubules in birds Reviewed

    Tomomi Ito, Norio Yoshizaki, Toshinobu Tokumoto, Hiroko Ono, Takashi Yoshimura, Akira Tsukada, Norio Kansaku and Tomohiro Sasanami

    Endocrinology   Vol. 152   page: 3952–3962   2011

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  4. Alleviation of body weight loss by dietary methionine is independent of insulin-like growth factor-I in protein-starved young chickens. Reviewed

    Kenji NAGAO, Mai OKI, Akira TSUKADA and Kazumi KITA

    Animal Science Journal   Vol. 82   page: 560–564   2011

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  5. Oocytic expression of zona pellucida protein ZP4 in Japanese quail (Coturnix japonica) Reviewed

    Serizawa, Mami; Kinoshita, Mihoko; Rodler, Daniela; Tsukada, Akira, Ono, Hiroko; Yoshimura, Takashi; Kansaku, Norio; Sasanami, Tomohiro

    Animal Science Journal   Vol. 82   page: 227–235   2011

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  6. Isolation and characterisation of prolactin-releasing peptide in chicks and its effect on prolactin release and feeding behaviour. Reviewed

    Tetsuya Tachibana, Shunsuke Moriyama, Akiyoshi Takahashi, Akira Tsukada, Aiko Oda, Sakae Takeuchi, Tatsuya Sakamoto

    Journal of Neuroendocrinology   Vol. 23   page: 74-81   2011

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    DOI: 10.1111/j.1365-2826.2010.02078.x.

  7. Effects of Insufficient Levels of Dietary Protein on IGF-I and IGFBPs in Young Chickens Reviewed

    Kenji Nagao, Kohzy Hiramatsu, Akira Tsukada and Kazumi Kita

    The Journal of Poultry Science   Vol. 47   page: 236-239   2010.7

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  8. Zona pellucida protein ZP2 is expressed in the oocyte of Japanese quail (Coturnix japonica) Reviewed

    Mihoko Kinoshita, Daniela Rodler, Kenichi Sugiura, Kayoko Matsushima, Norio Kansaku, Kenichi Tahara, Akira Tsukada, Hiroko Ono, Takashi Yoshimura, Norio Yoshizaki, Ryota Tanaka, Tetsuya Kohsaka and Tomohiro Sasanami

    Reproduction   Vol. 139   page: 359-371   2010

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  9. Novel method of gene transfer in birds: Intracytoplasmic sperm injection (ICSI) for green fluorescent protein (GFP) expression in quail blastoderms Reviewed

    Shusei Mizushima, Soichi Takagi, Tamao Ono, Yusuke Atsumi, Akira Tsukada, Noboru Saito, Tomohiro Sasanami, Masaru Okabe and Kiyoshi Shimada

      Vol. 83   page: 965-969   2010

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  10. *Phospholipase Cζ mRNA expression and its potency during spermatogenesis for activation of quail oocyte as a sperm factor Reviewed

    Mizushima, Shusei, Takagi, Soichi, Ono, Tamao, Atsumi, Yusuke, Tsukada, Akira, Saito, Noboru, Shimada, Kiyoshi

    Molecular Reproduction and Development   Vol. 76 ( 12 ) page: 1200-1207   2009.11

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  11. *Identification of two types of growth hormone receptor mutations in two strains of sex-linked dwarf chickens Reviewed

    Kenichi Tahara, Akira Tsukada, Takanobu Hanai, Kenta Okumura, Kikumi Yamada, Atsushi Murai, Rikiya Yamamoto, Makoto Maeno, Noboru Saito, Kiyoshi Shimada.

    The Journal of Poultry Science   Vol. 46   page: 249-256   2009.6

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

  12. Changes in post-translational modifications of prolactin during development and reproductive cycles in the chicken. Reviewed

    Gen Hiyama, Norio Kansaku, Mihoko Kinoshita, Tomohiro Sasanami, Akihiro Nakamura, Kenji Noda, Akira Tsukada, Kiyoshi Shimada, David Zadworny

    General and Comparative Endocrinology   Vol. 161   page: 238–245   2009.4

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  13. Molecular characterization of egg envelope glycoprotein ZPD in the ovary of Japanese quail (Coturnix japonica) Reviewed

    Tsukasa Sato, Mihoko Kinoshita, Norio Kansaku, Kenichi Tahara, Akira Tsukada, Hiroko Ono, Takashi Yoshimura, Hideo Dohra and Tomohiro Sasanami

    Reproduction.   Vol. 137   page: 333-343   2009.2

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  14. Developmental Enhancement of Intracytoplasmic Sperm Injection (ICSI)-Generated Quail Embryos by Phospholipase Cζ cRNA Reviewed

    Shusei Mizushima, Soichi Takagi, Tamao Ono, Yusuke Atsumi, Akira Tsukada, Noboru Saito and Kiyoshi Shimada

    Journal Poultry Science   Vol. 45   page: 152-158   2008.4

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  15. Effects of Aromatase Inhibitor (Fadrozole)-Induced Sex-Reversal on Gonadal Differentiation and mRNA Expression of P450arom, AMH and ERα in Embryos and Growth in Posthatching Quail Reviewed

    Naomi Koba, Masahiko Mori, Yonju Ha, Shusei Mizushima, Akira Tsukada, Noboru Saito, Tamao Ono and Kiyoshi Shimada

    Journal Poultry Science   Vol. 45   page: 116-124   2008.4

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  16. Profiles of mRNA Expression of FOXL2, P450arom, DMRT1, AMH, P450c17, SF1, ERα and AR, in Relation to Gonadal Sex Differentiation in Duck Embryo. Reviewed

    Naomi Koba, Toshimichi Ohfuji, Yonju Ha, Shusei Mizushima, Akira Tsukada, Noboru Saito and Kiyoshi Shimada

    Journal Poultry Science   Vol. 45   page: 132-138   2008.4

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  17. Predominant expression of growth hormone secretagogue receptor in the caudal lobe of chicken pituitary and parallel developmental increase in expression of pituitary GH and proventriculus ghrelin mRNA. Reviewed

    Ichiro YAMAMOTO, Yukinori YOSHIMURA, Akira TSUKADA, Norio KANSAKU, Nobumichi TSUSHIMA and Minoru TANAKA.

    Animal Science Journal   Vol. 79   page: 116-121   2008

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  18. Identification of differentially expressed genes involved in the regression and development of the chicken Mullerian duct.Int J Dev Biol. Reviewed

    Ha Y, Tsukada A, Saito N, Zadworny D, Shimada K.

    THE INTERNATIONAL JOURNAL OF DEVELOPMENTAL BIOLOGY   Vol. 52 ( 8 ) page: 1135-1141   2008

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  19. Expression of P450arom, AMH and ER-alpha mRNA in Gonads of Turkey, Duck and Goose within One Week of Age. Reviewed

    Naomi Koba, Toshimichi Ohfuji, Yonju Ha, Shusei Mizushima,Akira Tsukada, Noboru Saito and Kiyoshi Shimada

    The Journal of Poultry Science   Vol. 45   page: 220-226   2008

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  20. Incorporation of ZP1 into perivitelline membrane after in vivo treatment with exogenous ZP1 in Japanese quail (Coturnix japonica) Reviewed

    Mihoko Kinoshita, Kaori Mizui, Tsukasa Ishiguro, Mamoru Ohtsuki, Norio Kansaku, Hiroshi Ogawa, Akira Tsukada, Tsukasa Sato and Tomohiro Sasanami

    The FEBS Journal   Vol. 275 ( 14 ) page: 3580-3589   2008

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  21. Primary structure, tissue distribution, and biological activity of chicken motilin receptor. Reviewed

    Ichiro Yamamoto, Hiroyuki Kaiya, Chihiro Tsutsui, Takafumi Sakai, Akira Tsukada, Mikiya Miyazato, Minoru Tanaka

    General and Comparative Endocrinology   Vol. 156   page: 509-514   2008

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  22. Z-chromosome specific primers for chicken-quail hybrid blastoderm. Reviewed

      Vol. 44 ( 2 ) page: 209-212   2007.4

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  23. Fertilization and blastoderm development of quail oocytes after intracytoplasmic injection of chicken sperm bearing a W chromosome. Reviewed

      Vol. 86 ( 4 ) page: 731-738   2007

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  24. Fertilizing ability of chicken sperm bearing W chromosome. Reviewed

    Takagi S., Tsukada A., Saito N. and Shimada K.

    Poultry Science   Vol. 86 ( 5 ) page: 937-943   2007

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    In the sex-reversed domestic fowl, spermatids and sperm carrying the female-specific W chromosome have been demonstrated, but whether the spermatids can become functional sperm and can fertilize the oocyte remains undetermined. In the present study, sex reversal was induced by injection of a nonsteroidal aromatase inhibitor (Fadrozole) into the air sac of the chicken egg on d 4 of incubation, and the chicks were reared to 18 mo old. A single elongated spermatid or sperm was isolated from the testis from either normal roosters or sex-reversed hens, and each was microinjected into a mouse oocyte and cultured for 24 h. Although injected oocytes were monitored on the stage of microscope, they were classified into groups by the number of pronuclei. Those that showed male and female pronuclei (2PN) were considered to have oocyte-activating potency. In the normal rooster group, most semen and testicular sperm induced 2PN, whereas only half of the elongated spermatids induced 2PN. In the sex-reversal group, most testicular sperm induced 2PN, whereas nearly half of the elongated spermatids induced 2PN in the oocytes. There was no pronucleus in the oocytes after microinjection of medium only. A second experiment confirmed the higher rate of oocyte activation by testicular sperm than testicular elongated spermatids. In this second experiment, individual oocytes injected with spermatids and sperm of sex-reversed hens were assayed by PCR to identify the W chromosome. Most spermatids and sperm carried Z chromosome, whereas a minority carried W chromosome. However, the sperm carrying W chromosome evoked 2PN with the same rate of oocyte activation as those carrying Z chromosome. From these results, it is concluded that the chicken elongated spermatids and sperm carrying W chromosome may possess a fertilizing ability similar to normal chicken sperm carrying the Z chromosome.

  25. Primary Structure And Tissue Distribution Of GPR39 mRNA In Japanese Quail,Coturnix japonica. Reviewed

    I. Yamamoto, Y. Sakaguchi, M. Numao, A. Tsukada, N. Tsushima and M. Tanaka

    Poultly Sience   Vol. 2007 ( 86 ) page: 2472-2476   2007

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  26. Possible Role of Calcium on Oocyte Development After Intracytoplasmic Sperm Injection in Quail (Coturnix japonica) Reviewed

    SHUSEI MIZUSHIMA, SOICHI TAKAGI, TAMAO ONO, YUSUKE ATSUMI, AKIRA TSUKADA, NOBORU SAITO, AND KIYOSHI SHIMADA

    JOURNAL OF EXPERIMENTAL ZOOLOGY   Vol. 307A   page: 1-7   2007

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  27. Plasma Prolactin and Corticosterone Concentrations Are Changing Toward hatch with a Different Manner Between Layer- and Broiler- Type Chickens. Reviewed

    M. Sato, T. Tachibana, A. Tsukada and M. Furuse.

    Journal of Animal and Veterinary Advances   Vol. 6 ( 8 ) page: 923-926   2007

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  28. Identification of spermatogenic cells expressing protamine mRNA in Japanese quail by RT-PCR.

    "Upi Chairun Nisa, Akira Tsukada, Soichi Takagi, Ichiro Yamamoto, Yonju Ha, Anna Hrabia, Noboru Saito and Kiyoshi Shimada."

    Journal of Poultry Science   Vol. 42(1)   page: 70-78.   2005.1

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  29. Comparison of mammalian prolactin-releasing peptide and Carassius RFamide for feeding behavior and prolactin secretion in chicks. Reviewed

    Tetsuya Tachibana, Akira Tsukada, Masaaki Fujimoto, Hirokazu , Takahashi, Takeshi Ohkubo, Tim Boswell, Mitsuhiro Furuse.

    General and Comparative Endocrinology   Vol. 144   page: 264-269   2005

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  30. Changes in mRNA expression of MMP-2 in the M殕lerian duct of chicken Embryo.

    "Ha, Y., Tsukada,A., Sato, N. and Shimada, K."

    Gen Comp Endocrinol.   Vol. 139(2)   page: 131-136.   2004.12

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  31. Photoinducible phase specific light induction of Cry1 gene in the pars tuberalis of Japanese quail

    "Yasuo S, Watanabe M, Tsukada A, Takagi T, Iigo M, Shimada K, Ebihara S, Yoshimura T"

    Endocrinology   Vol. 145   page: 1612-1616   2004.4

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  32. Effect of central administration of prolactin-releasing peptide on feeding in chicks

    "Tetsuya Tachibana, Shin Saito, Shozo Tomonaga, Tomo Takagi, Ei-Suke Saito, Tomonori Nakanishi, Tomoyuki Koutoku, Akira Tsukada, Takeshi Ohkubo, Tim Boswell and Mitsuhiro Furuse"

    Physiology & Behavior   Vol. 80   page: 713-719   2004.1

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  33. cDNA cloning of chicken orexin receptor and tissue distribution: sex dimorphic expression in chicken gonads

    "Takeshi Ohkubo, Akira Tsukada and K Shamoto"

    Journal of Molecular Endocrinology   Vol. 31   page: 499-508   2003.11

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  34. cDNA cloning and mRNA Expression of estrogen receptor alpha in the Japanese quail.

    "Kouhei Ichikawa, Ichiro Yamamoto, Akira Tsukada, Noboru saito and Kiyoshi Shhimada"

    Journal Poultry Science   Vol. 40   page: 121-129   2003.4

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  35. Effect of Endocrine Disrupters on mRNA Expression of Vitellogenin (VTG) II and Very Low Density Lipoprotein (apoVLDL) II in the Liver of Quail Embryos.

    "Kouhei Ichikawa, Yonju Ha, Akira Tsukada, Noboru saito and Kiyoshi Shhimada"

    Journal Poultry Science   Vol. 40   page: 45-52   2003.2

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  36. Profiles of mRNA expression of genes related to sex differentiation of the gonads in the chicken embryo

    "Ichiro Yamamoto, Akira Tsukada, Noboru Saito and Kiyoshi Simada"

    Poult ry Science   Vol. 82   page: 1462-1467   2003.1

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  37. Expression of protamine mRNA in relation to spermatogenic activity in Japanese quail

    "Upi Chairun Nisa, Akira Tsukada, Masahiko Mori, Noboru Saito and Kiyoshi Shimada"

    Journal of Poultry Science   Vol. 40   page: 309-318   2003.1

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  38. "Effect of Estradiol and Nonylphenol on mRNA Expression of Estrogen Receptors α and β, and Cytochrome P450 Aromatase in the Gonad of Chicken Embryo."

    "Masanori Sakimura, Akira Tsukada, Makoto Usami, Shozo Hanzawa, Noboru Saito, Yasuo Ohno and Kiyoshi Shimada."

    Journal Poultry Science   Vol. 39   page: 302-309   2002.10

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  39. cDNA cloning and mRNA expression Transformer 2.(Tra2) in chicken embryo.

    "I. Yamamoto, A. Tsukada, N. Saito, K. Simada"

    Biochimica et Biophysica Acta   Vol. 1579   page: 185-188   2002.9

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  40. Suppressive effect of p-nonylphenol on male-specific mRNA expression in the embryonic gonad of chickens.

    "Sakimura M, Usami M, Hanzawa S, Tsukada A, Saito N, Yasuo Ohno and Shimada K."

    Journal of poultry science   Vol. 39   page: 91-99   2002.4

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  41. Effect of estradiol and nonylphenol on mRNA levels of vitellogeninII in the liver of chicken embryos.

    "Sakimura M., Hanazawa S., Tsukada A., Yamamoto I., Saito N., Usami M., Ohno Y., Shimada K."

    J. Poult. Sci.   Vol. 38   page: 250-257   2001.1

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  42. Effects of thyroid hormone on serum insulin-like growth factor binding proteins (IGFBPs) and IGFBP-2 mRNA expression in chickens. Reviewed

    "Akira Tsukada, Takeshi Ohkubo, Minoru Tanaka, Kunio Nakashima, Masaaki Wakita and Sadao Hoshino"

    Japanese poultry science   Vol. 36   page: 155-166   1999.5

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  43. Cloning and expression of pigeon groth hormone receptor cDNA in COS-7 monkey kidney cells1. Reviewed

    "Takeshi Ohkubo, Akira Tsukada, Minoru Tanaka and Kunio Nakashima"

    Comparative Biochemistry and Physiology part B.   Vol. 120   page: 449-455   1998.7

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  44. Thyroid hormones are involved in insulin-like growth factor-I (IGF-I) production by stimulating hepatic growth hormone receptor (GHR) gene expression in the chicken. Reviewed

    Akira Tsukada, Takeshi Ohkubo, Kesami Sakaguchi, Minoru Tanaka, Kunio Nakashima, Yukinobu Hayashida, Masaaki Wakita and Sadao Hoshino

    Growth hormone and IGF research   Vol. 8   page: 235-242   1998.6

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    Effect of thyroid status on IGF-I production in growing chickens was studied. Serum concentrations of GH were not affected by propylthiouracil (PTU) or thyroxine (T4) treatments, whereas serum IGF-I levels were significantly decreased in PTU-treated chickens. The lowered serum IGF-I levels in the PTU-treated group were completely restored to the control levels by T4 injections. In the liver, the messenger RNA (mRNA) expressions both for GH receptor (GHR) and IGF-I were significantly repressed by PTU treatment, and were restored again by T4 replacement. In addition, the results of analysis on radiolabelled GH binding to the liver membrane were consistent with the levels of hepatic GHR mRNA expression. Serum concentrations of IGF-I were positively correlated with hepatic IGF-I mRNA and GHR mRNA expressions. The correlation coefficient between serum T3 levels and hepatic IGF-I mRNA expressions was also significant. These results indicate that thyroid hormones regulate IGF-I production in the chicken by affecting hepatic GHR expression.

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Books 4

  1. In vivo and in vitro screening of endocrine disrupting chemicals with estrogenic activity in Japanese quail.

    Kiyoshi Shimada, Yonju Ha, Akira Tsukada, Noboru Saito, Shinobu Maekawa, Makoto Nishizaka and Masayoshi Imagawa.( Role: Joint author)

    Avian and poultry biology reviews  2005 

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    Language:English

  2. Effects of thyroid hormone on insulin-like growth factor (IGF) -I and IGF binding proteins (IGFBPs) in chickens

    Akira Tsukada, Kesami Sakaguchi, Minoru Tanaka, Kunio Nakashima, Masaaki Wakita and Sadao Hoshino.( Role: Joint author)

    Advances in comparative endocrinology Proceeding of comparative endocrinology Yokohama (Japan)  1997 

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  3. Advances in comparative endocrinology

    Akira Tsukada, Kesami Sakaguchi, Minoru Tanaka, Kunio Nakashima, Masaaki Wakita and Sadao Hoshino(EDITOR:Seiichiro Kawashima and Sakae Kikuyama)( Role: Joint author)

    Monduzzi Editore S.p.A-Bologona  1997 

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    Effects of thyroid hormone on insulin-like growth factor (IGF) -I and IGF binding proteins (IGFBPs) in chickens.

  4. Growth hormone receptor-dependent expression of IGFBP-4 mRNA in chicken liver.

    "Minoru Tanaka, Sadao Hoshino, Kunio Nakashima, Takeshi Ohkubo, Akira Tsukada, Masaaki Wakita"( Role: Joint author)

    1999.1 

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    Language:English

    Growth hormone and IGF research P-38

Presentations 48

  1. ウズラDAZL(deleted in azoospermia-like)mRNAの3’-UTRに結合するタンパク質の解析

    〇水島秀成・塚田光・笹浪知宏・小野珠乙・黒岩麻里

    第45回鳥類内分泌研究会  2021.12.4  鳥類内分泌研究会

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    Event date: 2021.12

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:リモート会議  

  2. 伴性型矮性ニワトリの血糖調節と血中脂質の調査

    塚田光・香取尚久・小酒井貴晴

    日本家禽学会 

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    Event date: 2019.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:麻布大学   Country:Japan  

  3. Central injection of prolactin-releasing peptide stimulates feeding behavior in chicks

    Tetsuya Tachibana1,*, Akira Tsukada2, Shunsuke Moriyama3, Tatsuya Sakamoto4

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    Event date: 2014.11

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  4. Growth hormone receptor signalling in chicken International conference

    Akira Tsukada, Ken-ichi Tahara

    17th ICCE 2013 - International Congress of Comparative Endocrinology 

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    Event date: 2013.7

    Language:English   Presentation type:Poster presentation  

    Country:Spain  

  5. Growth Hormone Dependency Testicular Gene Expression in Sex-linked Dwarf Chicken. International conference

    Riki Kuwahara, Manabu Sahashi and Akira Tsukada

    THE 15th AAAP ANIMAL SCIENCE CONGRESS 

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    Event date: 2012.11

    Language:English   Presentation type:Poster presentation  

    Country:Thailand  

  6. 免疫器官へのGH作用の解明

    ○塚田光,蔵岡良之,田原謙一,桑原力,村井篤嗣(名古屋大・生命農)

    第35回鳥類内分泌研究会 

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    Event date: 2010.11

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  7. 矮性鶏をもちいた成長ホルモン受容体機能の解明

    塚田光

    名城大学農学部特別講演会 

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    Event date: 2010.9

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  8. ニワトリの精巣・卵巣における成長ホルモン受容体 mRNA発現

    ◎桑原力1・塚田光1・山川智行1・蔵岡良之1・田中実2・島田清司1

    2010家禽学会春期大会 

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    Event date: 2010.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  9. 成長ホルモン受容体異常系統の作出と成長形質の評価

    ◎山川智行1・田原謙一1・蔵岡良之1・桑原力1・木下圭司2・水谷誠2・石川明1・並河鷹夫2・塚田光1・島田清司1(1名大院生命農・2名大院生命農ABRC)

    2010家禽学会春期大会 

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    Event date: 2010.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  10. 成長ホルモン受容体異常ニワトリコンジェニック系統の作出と成長形質の評価

    ○山川智行・田原謙一・蔵岡良之・桑原力・木下圭司・水谷誠・石川明・並河鷹夫・塚田光

    東海畜産学会 

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    Event date: 2009.12

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  11. The role of growth hormone in chicken glucose metabolism

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    Event date: 2009.11

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  12. ニワトリプロラクチン受容体の遺伝子構造と多重第一エキソンによる発現変動調節

    桑原力、田端秀美、和田智子、山本一郎、中尾愓宏、塚田光、田中実

    鳥類内分泌研究会 

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    Event date: 2009.11

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  13. ニワトリ成長ホルモン受容体細胞内情報伝達系の解明

    田原謙一・花井隆信・吉里秀雄・笹浪知宏・大久保武・齋藤昇・島田清司・○塚田光

    日本家禽学会 

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    Event date: 2009.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

    産業動物であるニワトリにおいて成長速度は重要な経済形質である。個体の成長を司るGH作用に関して、その細胞内情報伝達経路にJAK-STAT経路が重要であり、特にGHRのC末端約20残基が個体成長に重要であることをGH受容体欠損鶏、C末端異常鶏をもちいて明らかとした。

  14. 近交系ニワトリへの変異型成長ホルモン受容体遺伝子の導入と成長形質の評価

    ◯山川智行・田原謙一・奥村健太・山田規久美・水谷誠・並河鷹夫・齋藤昇・島田清司・塚田光

    日本家禽学会 

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    Event date: 2009.3

    Language:Japanese  

  15. ニワトリのリンパ器官における成長ホルモンの役割

    ○蔵岡良之・田原謙一・奥村健太・平松 宏明・伊藤 守弘・伊藤 康彦・本田 寛幸・村井 篤嗣・齋藤 昇・島田 清司・塚田 光

    日本家禽学会 

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    Event date: 2009.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

    GH作用を受容するGH受容体は個体の成長に重要である。GH受容体欠損鶏において免疫系組織が有意に減少することを明らかとし、それらの組織がGHの標的組織であることを確認した。GHR欠損系ではIgY濃度の低下や、抗体産生能の低下を引き起こし、この原因が胸腺、ファブリキウス嚢での免疫担当細胞の減少によることを明らかとした。

  16. ニワトリにおけるGH作用欠損によるインスリンシグナル及びインスリン分泌への影響

    ◯奥村健太・田原謙一・村井篤嗣・堀尾文彦・齋藤昇・島田清司・塚田光

    日本家禽学会 

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    Event date: 2009.3

    Language:Japanese  

    GH作用を受容するGH受容体は個体の成長に直接関わる骨、筋肉の他にも多くの組織で発現し、その作用も多様であることが示唆されている。GH作用がないニワトリでインスリン感受性が上昇すること明らかとした。また血中インスリンが低下し、その原因が膵臓でのベータ細胞の減少であることを明らかとした。

  17. GHR欠損(矮性)ニワトリを用いた肝臓でのJAK2-STAT5シグナル伝達様式の解析

    ○田原謙一、吉里秀雄、笹浪知宏、奥村健太、山田規久美、齋藤昇、島田清司、塚田光.

    第33回鳥類内分泌研究会 

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    Event date: 2008.11

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  18. ニワトリにおける成長ホルモンのインスリン抵抗性誘導作用に関する研究

    ◯奥村健太1)・田原謙一1)・山中貴達・1)・村井篤嗣1)・堀尾文彦1)・齋藤昇1)・島田清司2)・塚田光1)

    東海畜産学会 

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    Event date: 2008.11

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  19. ニワトリの免疫系における成長ホルモン(GH)の役割

    ○蔵岡良之1)・田原謙一1)・平松 宏明2)・伊藤 守弘2)・伊藤 康彦2) ・本田 寛幸1)・村井 篤嗣1)・齋藤 昇1)・島田 清司3) ・塚田 光1)

    東海畜産学会 

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    Event date: 2008.11

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  20. cDNA CLONING AND mRNA EXPRESSION PROFILES OF CHICKEN NEUROMEDIN U RECEPTORS (NMUR1 and NMUR2) International conference

    European Society of Veterinary Clinical Pathology & International Society for Animal Clinical Pathology CongressSept 30 –Oct 3rd 

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    Event date: 2008.9

    Language:English   Presentation type:Poster presentation  

  21. 成長ホルモン異常鶏におけるインスリン感受性の変化

    奥村健太、田原謙一、山中貴達、村井篤嗣、堀尾文彦、齋藤昇、島田清司、○塚田光

    日本家禽学会秋季大会 

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    Event date: 2008.8

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  22. Insulin-like growth factor binding protein 4 (IGFBP4) gene is induced by growth hormone activation via tandem Stat5 response elements in intron1 in chicken. International conference

    9th International Symposium on Avian Endocrinology. Leuven, Belgium (2008.7.11-15) 

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    Event date: 2008.7

    Language:English   Presentation type:Poster presentation  

  23. Cloning and characterization of the chicken Insulin-like growth factor binding protein 4 (IGFBP4) gene. International conference

    9th International Symposium on Avian Endocrinology. Leuven, Belgium (2008.7.11-15) 

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    Event date: 2008.7

    Language:English   Presentation type:Poster presentation  

  24. DIRECT ROLE FOR GROWTH HORMONE IN REGULATING INSULIN-LIKE GROWTH FACTOR BINDING PROTEIN 4 (IGFBP4) EXPRESSION IN CHICKEN International conference

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    Event date: 2007.9

    Language:English   Presentation type:Poster presentation  

  25. Development engineering for sex manipulation in domestic fowls. International conference

    XVI International Symposium of the polish network of molecular and cellular biology, ''Molecular and Physiological Aspects of Regulatory Processed of the Organism'' (June 2007), Pedagogical University of Cracow Poland, June 4-6, 420-421. 

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    Event date: 2007.6

    Language:English  

  26. Effect of strontium and calcium on ICSI-associated fertilization in quail.

    Proceedings of 8th Asian Pacific Poultry Conference (March 2007), Bangkok Thailand, 503-504. 

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    Event date: 2007

    Language:English   Presentation type:Poster presentation  

  27. Possible role of intracellular calcium release for fertilization in quail oocyte. International conference

    Proceedings of XIIth AAAP Animal Science Congress (September 2006), Busan Korea, CD-ROM. 

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    Event date: 2006.9

    Language:English   Presentation type:Oral presentation (general)  

  28. Aromatase, estrogen receptor α (ERα) and Anti-Mullerian hormone (AMH) mRNA expression of gonads at hatching and 1-week old in turkey and goose. International conference

    Proceedings of the 5th Intercongress Symposium of the Asia and Oceania Society for Comparative Endocrinology (February 2006), Bangkok Thailand, 359-361. 

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    Event date: 2006.2

    Language:English   Presentation type:Oral presentation (general)  

  29. Growth hormone induced insulin-like growth factor binding protein 4 gene activation in chicken

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    Event date: 2005.11

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  30. Growth hormone induced insulin-like growth factor binding protein 4 gene activation in chicken

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    Event date: 2005.11

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  31. Influence of prolactin genotype on performance in Nagoya Breed

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    Event date: 2005.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  32. 飼料中のMet及びGly欠乏がニワトリの筋肉蛋白質分解及び血中IGF-I濃度に及ぼす影響

    喜多一美・大木麻衣・長尾健二・塚田光・横田浩臣(名大院生命農)・大塚彰・林国興(鹿児島大農)

    日本畜産学会 第104回大会 東京 東京大学農学部 (2005) 3月27-29日 

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    Event date: 2005.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  33. 成長に伴う雄マガモ雛の羽装変化とその要因

    渡邊潤、柿崎美代、青山真人、塚田光、杉田昭栄

    日本畜産学会 第104回大会 東京 東京大学農学部 (2005) 3月27-29日 

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    Event date: 2005.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  34. Characterization of the chicken Insulin-like growth factor binding protein-4 gene regulatory region.

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    Event date: 2004.11

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  35. Effect of culture time after ICSI and combination of gamete sex on fertilization rate between chicken sperm and quail oocyte.

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    Event date: 2004.11

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  36. mRNA expression of P450arom, ERα and AMH in gonads of turkey poults and ducklings.

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    Event date: 2004.11

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  37. Cloning and mRNA tissue distribution of chicken plc-zeta.

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    Event date: 2004.11

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  38. アヒルにおける反復DNA 配列の解析

    小川 文・塚田 光・檜山源・岡林寿人・泉徳和・島田清司・○神作宜男

    第29回 鳥類内分泌研究会 厚木市(神奈川) (2004) 11月3-4日 

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    Event date: 2004.11

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  39. Clock genes and photoperiodism in Japanese quail International conference

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    Event date: 2004.6

    Language:English   Presentation type:Poster presentation  

  40. 鳥類の時計遺伝子と光周性

    安尾しのぶ、渡邊美和、塚田光、島田清司、海老原史樹文、吉村崇

    日本畜産学会 第103回大会 府中市 (東京)東京農工大学農学部 (2004) 3月29-31日 

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    Event date: 2004.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  41. 雄マガモの羽装の季節的二型性に関する研究

    渡邊潤、柿崎美代、今井真智子、青山真人、塚田光、杉田昭栄

    日本家禽学会2004年度春季大会 府中市(東京)東京農工大学農学部(2004)3月28日 

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    Event date: 2004.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  42. CHANGES IN mRNA EXPRESSION OF ERα AND MMP2 IN RELATION TO ASYMMETRICAL DEVELOPMENT OF OVIDUCT OF CHICKEN EMBRYO International conference

    AOSCE Nara, March 26-30, 2004 

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    Event date: 2004.3

    Language:English   Presentation type:Poster presentation  

    Country:Japan  

  43. PROTAMINE mRNA EXPRESSION IN DIFFERENT SPERMATOGENIC ACTIVITY IN JAPANESE QUAIL International conference

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    Event date: 2004.3

    Language:English   Presentation type:Poster presentation  

    Country:Japan  

  44. Fertilization of quail oocyte after intracytoplasmic injection of chicken sperm.

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    Event date: 2003.11

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  45. Effects of aromatase inhibitor on mRNA expression of P450arom, AMH and ERα in gonads of quail embryos.

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    Event date: 2003.11

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  46. Characterization of the chicken Insulin-like growth factor binding protein-4 gene structure.

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    Event date: 2003.11

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  47. Involvement of MMP2 in relation to regression of ovary and oviduct in chicken embryo.

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    Event date: 2003.11

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  48. Study on polyspermy using ICSI method in Japanese quail (Coturnix japonica).

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    Event date: 2003.11

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

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Works 2

  1. 成長ホルモンでニワトリを大きくする

    2011.5

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    20110511friに岐阜新聞に掲載『成長ホルモンでニワトリを大きくする』

  2. ニワトリの大きさを調節する成長ホルモン

    2010.10

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    20101025に鶏鳴新聞に掲載『ニワトリの大きさを調節する成長ホルモン』

Research Project for Joint Research, Competitive Funding, etc. 6

  1. ニワトリ・ウズラリソースの収集・保存・提供

    2022.4 - 2026.3

    ナショナルバイオリソースプロジェクト 

    塚田 光

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    Grant type:Competitive

  2. ニワトリ新規ゲノム育種マーカーの開発事業

    2022.4 - 2022.3

    日本中央競馬会畜産振興事業 

    塚田 光

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    Authorship:Coinvestigator(s)  Grant type:Competitive

  3. 地場産業の国際競争力強化につなげる東海発ゲノム育種: 鳥類 バイオサイエンスの展開と家禽の新規系統樹立をめざして

    2021.10 - 2022.3

    東海国立大学機構大学横断研究推進プロジェクト 

    塚田 光

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    Authorship:Coinvestigator(s)  Grant type:Competitive

    Grant amount:\320000 ( Direct Cost: \320000 )

  4. 成長ホルモンのインスリン抵抗性誘導メカニズムの解明

    2008.4 - 2009.3

    名古屋大学学術振興基金(学術研究助成) 

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    Grant type:Competitive

     成長ホルモン(GH)は古くから知られている下垂体ホルモンであり、GHはインスリン様成長因子を介し正常な個体の発育に寄与することがよく知られている。またその作用が多様であることも古くから示唆されている(インスリン感受性低下誘導、脂質分解、ナトリウムの貯留、免疫活性の促進)。しかしながら、その分子メカニズムは不明のままである。
     本申請では特にGHのインスリン感受性低下誘導作用に着目し、その分子メカニズムを明らかにするものである。成長ホルモンを過剰に分泌する、巨人症や末端肥大症患者は、糖尿病を誘発することが古くから知られており、古くは下垂体除去手術による治療が施されていた。近年の研究によりGH受容体 (GHR)の細胞内情報伝達機構が明らかとなり、その伝達機構の一つがインスリン受容体のそれと競合することがわかってきた。また、GHR-KOマウスが作出され、GHにより調節される、糖・脂質代謝に関わる因子群が報告されている。
     自然発祥的な成長ホルモン受容体(GHR)異常はヒト(ラロン症候群)とニワトリにのみ報告がある。この系統はすでに同定済みである。この系統における生理的基礎データーを収集することは、その分子メカニズムの解明に有用なモデルを提唱することができ、産業動物の効率的な生産に貢献できる。さらに近年健康ブームにより、糖などの栄養素代謝機構へのGH作用メカニズムを解明する上で有用なモデルを提唱できる。このGHR欠損系統を用いてすでにインスリン抵抗性が有意に低下することを明らかにしている。この系統を用い(in vivo assay)、GH依存的でインスリン受容体細胞内情報伝達系因子群(IRS1,2,PI3K,Akt,PPARなど)、栄養素代謝(FABP,GAPDH,GK)に関与する因子群の同定が可能である。さらに肝細胞初代培養系を用いGH、インスリン添加群における糖の取り込み、関連因子群のリン酸化を含む感受性の評価が可能である(in vitro assay)。上述の因子群の感受性の変動、遺伝子発現の変化を評価することにより、成長ホルモン細胞増殖作用以外の個体の栄養素同化メカニズム(インスリン抵抗性誘導メカニズム)を明らかにできる。

  5. 鳥類の感染症抵抗性に関する国際共同研究

    2006.4 - 2007.3

    名古屋大学 総長裁量経費 教育研究改革・改善プロジェクト経費 

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    Grant type:Competitive

    名古屋大学 総長裁量経費 教育研究改革・改善プロジェクト経費:鳥類の感染症抵抗性に関する国際共同研究(分担)2006年

  6. ニワトリにおけるJAK-STATシグナル伝達系の解析

    2005.4 - 2006.3

    名古屋大学学術振興基金(学術研究助成) 

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    Grant type:Competitive

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KAKENHI (Grants-in-Aid for Scientific Research) 9

  1. インスリン感受性の低下における成長ホルモン作用メカニズム

    2016 - 2019.3

    科学研究費補助金  基盤研究(C)

    塚田光

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    Authorship:Principal investigator 

  2. 成長ホルモン受容体欠損鶏をもちいた成長ホルモン機能の網羅的解明

    2006.4 - 2008.3

    科学研究費補助金  若手研究(B)

    塚田 光

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    Authorship:Principal investigator 

  3. 鳥類の成長・分化に関わる成長因子の分子生物学的研究

    2002.4 - 2004.3

    科学研究費補助金  若手研究(B)

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    Authorship:Principal investigator 

    科研費

  4. 鳥類の成長・発育に関する成長因子の分子生物学的研究

    1999.4 - 2002.3

    科学研究費補助金  特別研究員奨励費

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    Authorship:Principal investigator 

    科研費

  5. ニワトリの水分摂取・排泄の生理機構解明と家禽管理への

    2005.4 - 2008.3

    科学研究費補助金  基盤研究(B)

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    Authorship:Coinvestigator(s) 

  6. 鳥類に特異的な左右非対称性卵巣・卵管分化に関する遺伝生理学的研究

    2005.4 - 2007.3

    科学研究費補助金  特定領域研究(1)

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    Authorship:Coinvestigator(s) 

  7. 筋肉蛋白質の蓄積促進に関わるIGF-Iシグナルカスケードの解明

    2003.4 - 2006.3

    科学研究費補助金  科学研究費補助金基盤研究(B)(1)

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    Authorship:Coinvestigator(s) 

  8. Mechanism of growth hormone action in the reduction of insulin sensitivity

    Grant number:16K07988  2016.10 - 2020.3

    Tsukada Akira

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    Authorship:Principal investigator 

    Grant amount:\4810000 ( Direct Cost: \3700000 、 Indirect Cost:\1110000 )

    A congenic line was created by backcrossing two GHR abnormal chickens to a GSP line (15th generation backcross). It is known that these two lines differ in GHR intracellular signal transduction involved in liver IGF-I mRNA synthesis. As a result of GTT and ITT, it was found that the insulin sensitivity was stronger in the phosphorylated alienation line of STAT5 only. That is, various signaling substances are expected to bind to the intracellular domain of GHR, and they act in a complicated manner. GH-dependent genes in the liver were comprehensively analyzed. Interesting genes such as GST, PLG, GLG-1, cEST159o8, DDX5, FGB, 2AB56, CALM2, C1S, CYP3A37, and COMT were found.

  9. 鳥類の浸透圧調節機構に関する研究

    2002.4 - 2005.3

    科学研究費補助金  科学研究費補助金基盤研究(B)(2)

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    Authorship:Coinvestigator(s) 

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Industrial property rights 1

  1. Japanese quail steroidogenic factor-I polypeptide, Japanese quail steroidogenic factor-I cDNA

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    Application no:2000100212 

 

Teaching Experience (On-campus) 61

  1. 修士論文研究4

    2022

  2. 修士論文研究3

    2022

  3. 修士論文研究2

    2022

  4. 修士論文研究1

    2022

  5. 生命農学演習4

    2022

  6. 生命農学演習3

    2022

  7. 生命農学演習2

    2022

  8. 生命農学演習1

    2022

  9. 農学セミナー

    2022

  10. 生物学実験

    2022

  11. 資源生物科学基盤実験実習

    2022

  12. 資源生物科学実験実習

    2022

  13. 動物生理学特論2

    2022

  14. 動物生理学2

    2022

  15. 動物生理学特論2

    2020

  16. 資源生物科学実験実習1

    2020

  17. 資源生物科学基盤実験実習

    2020

  18. 農学セミナー

    2020

  19. 資源生物科学基盤実験実習

    2018

  20. 資源生物科学実験実習

    2018

  21. 修士論文研究2

    2018

  22. 修士論文研究1

    2018

  23. 生命農学演習4

    2018

  24. 生命農学演習3

    2018

  25. 生命農学演習2

    2018

  26. 生命農学演習1

    2018

  27. 動物生理学特論2

    2018

  28. 専門セミナー

    2018

  29. 動物生理学2

    2018

     詳細を見る

    動物の生理学、特にニワトリ

  30. 生物学実験

    2018

     詳細を見る

    生物学実験

  31. 情報リテラシー

    2018

     詳細を見る

    情報の処理管理について

  32. 資源生物科学実験実習

    2017

  33. 資源生物科学基盤実験実習

    2017

  34. 生物学実験

    2017

     詳細を見る

    生物学実験

  35. 動物生理学3

    2017

     詳細を見る

    動物の生理学について特にニワトリの内分泌

  36. 情報リテラシー

    2017

     詳細を見る

    情報処理管理について

  37. 動物生理学3

    2016

     詳細を見る

    動物、ニワトリの生理学

  38. 資源生物科学セミナー

    2016

     詳細を見る

    輪講

  39. 生物学実験

    2016

     詳細を見る

    生物学実験

  40. 資源生物科学実験実習

    2016

  41. 資源生物科学基盤実験実習

    2016

  42. 生物学実験

    2015

     詳細を見る

    生物学実験

  43. 資源生物科学実験実習

    2015

  44. 資源生物科学基盤実験実習

    2015

  45. 資源生物科学セミナー

    2014

     詳細を見る

    輪講

  46. 動物生理学3

    2014

     詳細を見る

    サカナとニワトリの生理学

  47. 生物学実験

    2014

     詳細を見る

    生物学実験

  48. 資源生物科学実験実習

    2014

  49. 資源生物科学基盤実験実習

    2014

  50. 生物学実験

    2013

     詳細を見る

    生物学実験

  51. 資源生物科学実験実習

    2013

  52. 資源生物科学基盤実験実習

    2013

  53. 生物学実験

    2012

     詳細を見る

    生物学実験

  54. 資源生物科学実験実習

    2012

  55. 資源生物科学基盤実験実習

    2012

  56. 生物学実験

    2011

     詳細を見る

    生物学実験

  57. 資源生物科学実験実習

    2011

  58. 資源生物科学基盤実験実習

    2011

  59. 生物学実験

    2008

     詳細を見る

    生物学実験

  60. 資源生物科学実験実習

    2008

  61. 資源生物科学実験実習

    2006

▼display all

Teaching Experience (Off-campus) 1

  1. 解剖生理学

    2003.4 - 2004.3 名古屋経済短期大学 生活文化食物栄養)

 

Social Contribution 1

  1. Journal of Poultry Science

    Role(s):Editer

    2006.4