Updated on 2022/04/16

写真a

 
SATO, Chihiro
 
Organization
Institute for Glyco-core Research Professor
Graduate School of Bioagricultural Sciences Professor
Graduate School of Bioagricultural Sciences Professor
Graduate School
Graduate School of Bioagricultural Sciences
Title
Professor
Contact information
メールアドレス

Degree 1

  1. Ph D ( 1997.3   The University of Tokyo ) 

Research Interests 3

  1. Glycobiology, acidic sugar, sialic acid, polysialic acid, neuron, brain, gametic cell, raft, mental disorder, schizophrenia, autism, bipolar disorder

  2. bipolar disorder

  3. Glycobiology

Research Areas 4

  1. Life Science / Functional biochemistry  / Functional Biochemistry

  2. Life Science / Structural biochemistry  / Structured Biochemistry

  3. Life Science / Applied biochemistry

  4. Life Science / Functional biochemistry

Current Research Project and SDGs 3

  1. Structure and Function of Di/Oligo/Polysialic acid

  2. 疾患関連糖鎖の構造と機能

  3. 疾患に関わる糖鎖プローブの開発と利用

Research History 11

  1. Nagoya University   iGCORE   Professor

    2021.1

  2. Nagoya University   Integrated Glyco-Biomedical Research Center   Director in General

    2021.1

  3. 名古屋大学 統合生命科学糖鎖研究センター

    2021.1

  4. Nagoya University   Graduate School of Bioagricultural Sciences   Professor

    2019.10

  5. Nagoya University   Bioscience and Biotechnology Center   Associate professor

    2007.4 - 2019.9

  6. Associate Professor, Bioscience and Biotechnology Center, Nagoya University

    2007.4 - 2018.9

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    Country:Japan

  7. Bioscience and Biotechnology Center, Nagoya University/Associate Professor

    2005.2 - 2007.3

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    Country:Japan

  8. 名古屋大学大学院生命農学研究科 助手

    2001.9 - 2005.1

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    Country:Japan

  9. 名古屋大学生物分子応答研究センター 研究機関研究員

    2001.4 - 2001.8

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    Country:Japan

  10. 日本学術振興会 特別研究員

    1998.4 - 2001.3

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    Country:Japan

  11. 日本学術振興会 特別研究員

    1996.4 - 1998.3

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    Country:Japan

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Education 4

  1. The University of Tokyo   Graduate School of Science   Department of Biophysics and Biochemistry

    1994.4 - 1997.3

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    Country: Japan

  2. The University of Tokyo   Graduate School, Division of Science   Department of Biophysics and Biochemistry

    1994.4 - 1997.3

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    Country: Japan

  3. The University of Tokyo

    1992.4 - 1994.3

  4. The University of Tokyo   Faculty of Science   Department of Biophysics and Biochemistry

    1988.4 - 1992

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    Country: Japan

Professional Memberships 8

  1. 日本生化学会   評議員

    2021.1

  2. 日本糖質学会   評議員

    2005.4

  3. 日本生化学会   中部支部会幹事

    2012.9 - 2014.8

  4. 日本糖鎖科学コンソーシアム(JCGG)   評議員

    2018.4

  5. 日本農芸化学会

  6. 糖鎖科学名古屋拠点   事務局

    2003.4

  7. ASBMB

  8. 日本神経科学会

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Committee Memberships 8

  1. 日本生化学会   評議員  

    2021.1   

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    Committee type:Academic society

  2. 日本糖質学会   評議員  

    2005.4   

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    Committee type:Academic society

  3. 日本糖鎖科学コンソーシアム (JCGG)   評議員  

    2018.4   

  4. 日本生化学誌   企画委員  

    2017.9   

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    Committee type:Academic society

  5. 日本農芸化学会 化学と生物   編集員  

    2016.4 - 2021.3   

  6. 日本生化学会中部支部会   幹事  

    2012.9 - 2014.8   

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    Committee type:Academic society

  7. 日本農芸化学会   代議員  

    2009.4 - 2011.3   

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    Committee type:Academic society

  8. 糖鎖科学中部拠点   事務局  

    2003.4   

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    Committee type:Other

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Awards 4

  1. 日本糖質学会奨励賞

    2003   日本糖質学会  

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    Country:Japan

  2. 第1回日本農芸化学女性研究者賞

    2017.3   日本農芸化学会  

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    Award type:Award from Japanese society, conference, symposium, etc.  Country:Japan

  3. 第3回日本糖質学会ポスター賞

    1999  

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    Country:Japan

  4. Complex Formation of a Brain-Derived Neurotrophic Factor and Glycosaminoglycans.

    2010.3   Biosci. Biotech. and Biochem.  

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    Country:Japan

 

Papers 358

  1. Analysis of biochemical features of ST8 α-N-acetyl-neuraminide α2,8-sialyltransferase (St8sia) 5 isoforms.

    Araki E, Hane M, Hatanaka R, Kimura R, Tsuda K, Konishi M, Komura N, Ando H, Kitajima K, Sato C

    Glycoconjugate journal   Vol. 39 ( 2 ) page: 291 - 302   2022.4

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Glycoconjugate Journal  

    Gangliosides are important components of the membrane and are involved in many biological activities. St8sia5 is an α2,8-sialyltransferase involved in ganglioside synthesis, and has three isoforms. In this study, we analyzed the features of three isoforms, St8sia5-S, -M, and -L that had not been analyzed, and found that only St8sia5-L was localized in the Golgi, while the majority of St8sia5-M and -S were localized in the ER. The localization of Golgi of St8sia5 depended on the stem region. In addition, the incorporation of exogenous GD3 was upregulated only in St8sia5-L expressing cells. Taken together, the localization of St8sia5 is important for the activity of the enzyme.

    DOI: 10.1007/s10719-021-10034-8

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  2. The α2,8-sialyltransferase 6 (St8sia6) localizes in the ER and enhances the anchorage-independent cell growth in cancer.

    Hatanaka R, Araki E, Hane M, Go S, Wu D, Kitajima K, Sato C

    Biochemical and biophysical research communications   Vol. 608   page: 52 - 58   2022.3

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.bbrc.2022.03.146

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  3. A point-mutation in the C-domain of CMP-sialic acid synthetase leads to lethality of medaka due to protein insolubility.

    Wu D, Arakawa H, Fujita A, Hashimoto H, Hibi M, Naruse K, Kamei Y, Sato C, Kitajima K

    Scientific reports   Vol. 11 ( 1 ) page: 23211   2021.12

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Scientific Reports  

    Vertebrate CMP-sialic acid synthetase (CSS), which catalyzes the synthesis of CMP-sialic acid (CMP-Sia), consists of a 28 kDa-N-domain and a 20 kDa-C-domain. The N-domain is known to be a catalytic domain; however, the significance of the C-domain still remains unknown. To elucidate the function of the C-domain at the organism level, we screened the medaka TILLING library and obtained medaka with non-synonymous mutations (t911a), or single amino acid substitutions of CSS, L304Q, in the C-domain. Prominently, most L304Q medaka was lethal within 19 days post-fertilization (dpf). L304Q young fry displayed free Sia accumulation, and impairment of sialylation, up to 8 dpf. At 8 dpf, a marked abnormality in ventricular contraction and skeletal myogenesis was observed. To gain insight into the mechanism of L304Q-induced abnormalities, L304Q was biochemically characterized. Although bacterially expressed soluble L304Q and WT showed the similar Vmax/Km values, very few soluble L304Q was detected when expressed in CHO cells in sharp contrast to the WT. Additionally, the thermostability of various mutations of L304 greatly decreased, except for WT and L304I. These results suggest that L304 is important for the stability of CSS, and that an appropriate level of expression of soluble CSS is significant for animal survival.

    DOI: 10.1038/s41598-021-01715-3

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  4. Identification and functional characterization of a Siglec-7 counter-receptor on K562 cells. Reviewed International coauthorship

    Yoshimura A, Asahina Y, Chang LY, Angata T, Tanaka H, Kitajima K, Sato C

    The Journal of biological chemistry   Vol. 296   page: 100477   2021.1

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Journal of Biological Chemistry  

    Sialic acid (Sia)-binding immunoglobulin-like lectin 7 (Siglec-7) is an inhibitory receptor primarily expressed on natural killer (NK) cells and monocytes. Siglec-7 is known to negatively regulate the innate immune system through Sia binding to distinguish self and nonself; however, a counterreceptor bearing its natural ligand remains largely unclear. Here, we identified a counter-receptor of Siglec-7 using K562 hematopoietic carcinoma cells presenting cell surface ligands for Siglec-7. We affinity-purified the ligands using Fc-ligated recombinant Siglec-7 and diSia-dextran polymer, a strong inhibitor for Siglec-7. We then confirmed the counter-receptor for Siglec-7 as leukosialin (CD43) through mass spectrometry, immunoprecipitation, and proximity labeling. Additionally, we demonstrated that the cytotoxicity of NK cells toward K562 cells was suppressed by overexpression of leukosialin in a Siglec-7-dependent manner. Taken together, our data suggest that leukosialin on K562 is a counter-receptor for Siglec-7 on NK cells and that a cluster of the Sia-containing glycan epitope on leukosialin is key as trans-ligand for unmasking the cis-ligand.

    DOI: 10.1016/j.jbc.2021.100477

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  5. Lower promoter activity of the ST8SIA2 gene has been favored in evolving human collective brains.

    Hayakawa T, Terahara M, Fujito NT, Matsunaga T, Teshima KM, Hane M, Kitajima K, Sato C, Takahata N, Satta Y

    PloS one   Vol. 16 ( 12 ) page: e0259897   2021

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:PLoS ONE  

    ST8SIA2 is an important molecule regulating expression of the phenotype involved in schizophrenia. Lowered promoter activity of the ST8SIA2 gene is considered to be protective against schizophrenia by conferring tolerance to psychosocial stress. Here, we examined the promoter-type composition of anatomically modern humans (AMHs) and archaic humans (AHs; Neanderthals and Denisovans), and compared the promoter activity at the population level (population promoter activity; PPA) between them. In AMHs, the TCT-type, showing the second lowest promoter activity, was most prevalent in the ancestral population of non-Africans. However, the detection of only the CGT-type from AH samples and recombination tracts in AH sequences showed that the CGT- and TGT-types, exhibiting the two highest promoter activities, were common in AH populations. Furthermore, interspecies gene flow occurred into AMHs from AHs and into Denisovans from Neanderthals, influencing promoter-type compositions independently in both AMHs and AHs. The difference of promoter-type composition makes PPA unique in each population. East and Southeast Asian populations show the lowest PPA. This results from the selective increase of the CGC-type, showing the lowest promoter activity, in these populations. Every non-African population shows significantly lower PPA than African populations, resulting from the TCT-type having the highest prevalence in the ancestral population of non-Africans. In addition, PPA reduction is also found among subpopulations within Africa via a slight increase of the TCT-type. These findings indicate a trend toward lower PPA in the spread of AMHs, interpreted as a continuous adaptation to psychosocial stress arising in migration. This trend is considered as genetic tuning for the evolution of collective brains. The inferred promoter-type composition of AHs differed markedly from that of AMHs, resulting in higher PPA in AHs than in AMHs. This suggests that the trend toward lower PPA is a unique feature in AMH spread.

    DOI: 10.1371/journal.pone.0259897

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  6. The expression of sulfated sialic acids (SiaS) is dynamically regulated in mammalian cells

    Kitajima Ken, Ertunc Nursah, Sato Chihiro

    GLYCOBIOLOGY   Vol. 30 ( 12 ) page: 1066 - 1067   2020.12

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  7. Sialic acid sulfation is induced by the antibiotic treatment in mammalian cells.

    Ertunc N, Sato C, Kitajima K

    Bioscience, biotechnology, and biochemistry   Vol. 84 ( 11 ) page: 2311 - 2318   2020.11

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Bioscience, Biotechnology and Biochemistry  

    Sialic acids (Sias) are an outermost-situated sugar of glycoproteins and glycolipids to play important roles in various biological phenomena. They are often modified by additional substituents, such as O-acetyl group, to display more than 50 different structures in nature. Of those modified Sia, nothing is known about the occurrence and biological functions of sulfated Sias (SiaSs) in mammals. To elucidate the significance of sialic acid sulfation, we investigated various mammalian-cultured cell lines for the expression of SiaS using the specific antibody 3G9. First, SiaS is expressed in a cell line-dependent and a cell density-dependent manner. Second, in CHO cells, the expression of SiaS is reversibly induced by treatment with the antibiotic G418. Taken together, the expression of SiaS is changed by intrinsic and extrinsic factors in mammalian cells. This is the first demonstration of regulated expression of SiaS.

    DOI: 10.1080/09168451.2020.1792763

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  8. Systems glycomics of adult zebrafish

    Kitajima Ken, Yamakawa Nao, Sato Chihiro, Yann Guérardel

      Vol. 92 ( 3 ) page: 349 - 359   2020.6

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    Language:Japanese   Publishing type:Research paper (scientific journal)  

    DOI: 10.14952/seikagaku.2020.920349

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  9. Identification and characterization of a novel, versatile sialidase from a Sphingobacterium that can hydrolyze the glycosides of any sialic acid species at neutral pH. Reviewed

    Iwaki Y, Matsunaga E, Takegawa K, Sato C, Kitajima K

    Biochemical and biophysical research communications   Vol. 523 ( 2 ) page: 487 - 492   2020.3

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Biochemical and Biophysical Research Communications  

    Bacterial sialidases are widely used to remove sialic acid (Sia) residues from glycans. Most of them cleave the glycosides of N-acetylneuraminic acid (Neu5Ac) and N-glycolylneuraminic acid (Neu5Gc) under acidic pHs; however, currently available bacterial sialidases had no activity to the glycosides of deaminoneuraminic acid (Kdn). In this study, we found a novel sialidase from Sphingobacterium sp. strain HMA12 that could cleave any of the glycosides of Neu5Ac, Neu5Gc, and Kdn. It also had a broad linkage specificity, i.e., α2,3-, α2,6-, α2,8-, and α2,9-linkages, and the optimal pH at neutral ranges, pH 6.5–7.0. These properties are particularly important when sialidases are applied for in vivo digestion of the cell surface sialosides under physiological conditions. Interestingly, 2,3-didehydro-2-deoxy-N-acetylneuraminic acid (Neu5Ac2en), which is a transition state analog-based inhibitor, competitively inhibited the enzyme-catalyzed reaction for Kdn as well as for Neu5Ac, suggesting that the active site is common to the Neu5Ac and Kdn residues. Taken together, this sialidase is versatile and useful for the in vivo research on sialo-glycoconjugates.

    DOI: 10.1016/j.bbrc.2019.12.079

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  10. PSA-NCAM Colocalized with Cholecystokinin-Expressing Cells in the Hippocampus Is Involved in Mediating Antidepressant Efficacy. Reviewed

    Yamada J, Sato C, Konno K, Watanabe M, Jinno S

    The Journal of neuroscience : the official journal of the Society for Neuroscience   Vol. 40 ( 4 ) page: 825 - 842   2020.1

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Journal of Neuroscience  

    The extracellular glycan polysialic acid linked to neural cell adhesion molecule (PSA-NCAM) is principally expressed in the developing brain and the adult neurogenic regions. Although colocalization of PSA-NCAM with cholecystokinin (CCK) was found in the adult brain, the role of PSA-NCAM remains unclear. In this study, we aimed to elucidate the functional significance of PSA-NCAM in the CA1 region of the male mouse hippocampus. Combined fluorescence in situ hybridization and immunohistochemistry showed that few vesicular glutamate transporter 3-negative/CCK-positive (VGluT3 –/CCK +) cells were colocalized with PSA-NCAM, but most of the VGluT3 +/ CCK + cells were colocalized with PSA-NCAM. The somata of PSA-NCAM +/CCK + cells were highly innervated by serotonergic boutons than those of PSA-NCAM –/CCK + cells. The expression ratios of 5-HT3A receptors and p11, a serotonin receptor-interacting protein, were higher in PSA-NCAM +/CCK + cells than in PSA-NCAM –/CCK + cells. Pharmacological digestion of PSA-NCAM impaired the efficacy of antidepressant fluoxetine (FLX), a selective serotonin reuptake inhibitor, but not the efficacy of benzodiazepine anxiolytic diazepam. A Western blot showed that restraint stress decreased the expressions of p11 and mature brain-derived neurotrophic factor (BDNF), and FLX increased them. Interestingly, the FLX-induced elevation of expression of p11, but not mature BDNF, was impaired by the digestion of PSA-NCAM. Quantitative reverse transcription-polymerase chain reaction showed that restraint stress reduced the expression of polysialyltransferase ST8Sia IV and FLX elevated it. Collectively, PSA-NCAM colocalized with VGluT3 +/CCK + cells in the CA1 region of the hippocampus may play a unique role in the regulation of antidepressant efficacy via the serotonergic pathway.

    DOI: 10.1523/JNEUROSCI.1779-19.2019

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  11. Structure and Function of di/oligo/polysialic Acid (Polymerized Sialyl Structure) Invited

    Sato Chihiro

    Trends in Glycoscience and Glycotechnology   Vol. 31 ( 181 ) page: SJ32 - SJ35   2019.7

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    Language:Japanese   Publishing type:Research paper (scientific journal)   Publisher:FCCA(Forum: Carbohydrates Coming of Age)  

    <p>Sialic acid (Sia) is known to present as a monosialyl residue at a non-reducing terminal end of glycan in nature. In some cases, Sias occur as polymerized sialyl structures, di/oligo/polysialic acids (diSia/oligoSia/polySias). Here, we focus on the structure, function and biological significance of diSia/oligoSia/polySias. Following the definition of di/oligo/polySia structures and establishment of chemical and immunochemical detection methods, we analyzed these structures and their function based on a set of working hypotheses. So far, the biological meanings of degree of polymerization (DP) were shown and they lead to the change the concept of polySia. In addition, this research is applicable in the study of diseases such as mental disorder and cancer. However, research on the biological significance of the diversity in di/oligo/polySia structures is still ongoing.</p>

    DOI: 10.4052/tigg.1915.2sj

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  12. Structure and Function of di/oligo/polysialic Acid (Polymerized Sialyl Structure) Invited

    Sato Chihiro

    Trends in Glycoscience and Glycotechnology   Vol. 31 ( 181 ) page: SE32 - SE35   2019.7

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    Language:Japanese   Publishing type:Research paper (scientific journal)   Publisher:FCCA(Forum: Carbohydrates Coming of Age)  

    <p>Sialic acid (Sia) is known to present as a monosialyl residue at a non-reducing terminal end of glycan in nature. In some cases, Sias occur as polymerized sialyl structures, di/oligo/polysialic acids (diSia/oligoSia/polySias). Here, we focus on the structure, function and biological significance of diSia/oligoSia/polySias. Following the definition of di/oligo/polySia structures and establishment of chemical and immunochemical detection methods, we analyzed these structures and their function based on a set of working hypotheses. So far, the biological meanings of degree of polymerization (DP) were shown and they lead to the change the concept of polySia. In addition, this research is applicable in the study of diseases such as mental disorder and cancer. However, research on the biological significance of the diversity in di/oligo/polySia structures is still ongoing.</p>

    DOI: 10.4052/tigg.1915.2SE

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  13. Glycans in Nervous System Reviewed

    Sato Chihiro, Gu Jianguo, Fukuda Tomohiko, Kadomatsu Kenji, Ikenaka Kazuhiro

    GLYCOSCIENCE: BASIC SCIENCE TO APPLICATIONS: INSIGHTS FROM THE JAPAN CONSORTIUM FOR GLYCOBIOLOGY AND GLYCOTECHNOLOGY (JCGG)     page: 209 - 219   2019

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    Language:Japanese   Publishing type:Research paper (scientific journal)   Publisher:Glycoscience: Basic Science to Applications: Insights from the Japan Consortium for Glycobiology and Glycotechnology (JCGG)  

    DOI: 10.1007/978-981-13-5856-2_12

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  14. New Functions and Applications of an Acidic Glycan, Polysialic Acid: Repulsive and Attractive Fields of Polysialic Acid

    SATO Chihiro

    KAGAKU TO SEIBUTSU   Vol. 56 ( 6 ) page: 422 - 431   2018.5

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    Language:Japanese   Publishing type:Research paper (scientific journal)   Publisher:Japan Society for Bioscience, Biotechnology, and Agrochemistry  

    DOI: 10.1271/kagakutoseibutsu.56.422

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  15. Synthesis of end-functionalized glycopolymers containing alpha(2,8) disialic acids via pi-allyl nickel catalyzed coordinating polymerization and their interaction with Siglec-7 Reviewed

    Shuichi Ohira, Yu Yasuda, Ikuyoshi Tomita, Ken Kitajima, Takashi Takahashi, Chihiro Sato, Hiroshi Tanaka

    CHEMICAL COMMUNICATIONS   Vol. 53 ( 3 ) page: 553 - 556   2017.1

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:ROYAL SOC CHEMISTRY  

    An allene monomer containing an alpha(2,8) disialic acid was polymerized by a pi-allyl nickel complex with an azido group to produce end-functional glycopolymers with an excellent polydispersity index. The polymers allowed terminal modification with a fluorescent dye by click chemistry. The glycopolymers can dissociate Siglec-7-GD3 interactions at low concentrations.

    DOI: 10.1039/c6cc07115e

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  16. Polysialyltransferase ST8SIA2 and psychiatric disorders

    Hane Masaya, Kitajima Ken, Sato Chihiro

      Vol. 89 ( 5 ) page: 634 - 643   2017

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    DOI: 10.14952/SEIKAGAKU.2017.890634

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  17. ポリシアル酸転移酵素遺伝子ST8SIA2と精神疾患の関わり Reviewed

    羽根 正弥, 佐藤 ちひろ

    生化学   Vol. 89   page: 634 - 643   2017

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  18. Hsp70の酸性複合糖質結合活性の発見. 糖の意外な働き. Reviewed

    佐藤 ちひろ

    化学と生物   Vol. 55,   page: 22 - 26   2017

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  19. Binding Activity of Hsp70 toward Acidic Glycoconjugates

    HARADA Yoichiro, SATO Chihiro, KITAJIMA Ken

    KAGAKU TO SEIBUTSU   Vol. 55 ( 1 ) page: 22 - 26   2016

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    Language:Japanese   Publishing type:Research paper (scientific journal)   Publisher:Japan Society for Bioscience, Biotechnology, and Agrochemistry  

    DOI: 10.1271/kagakutoseibutsu.55.22

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  20. Protective effects of polysialic acid on proteolytic cleavage of FGF2 and proBDNF/BDNF.

    Hane M, Matsuoka S, Ono S, Miyata S, Kitajima K, Sato C

    Glycobiology   Vol. 25 ( 10 ) page: 1112 - 24   2015.10

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Glycobiology  

    Polysialic acid (polySia) is a linear polymer of sialic acid that modifies neural cell adhesion molecule (NCAM) in the vertebrate brain. PolySia is a large and exclusive molecule that functions as a negative regulator of cell-cell interactions. Recently, we demonstrated that polySia can specifically bind fibroblast growth factor 2 (FGF2) and BDNF; however, the protective effects of polySia on the proteolytic cleavage of these proteins remain unknown, although heparin/heparan sulfate has been shown to impair the cleavage of FGF2 by trypsin. Here, we analyzed the protective effects of polySia on the proteolytic cleavage of FGF2 and proBDNF/BDNF. We found that polySia protected intact FGF2 from tryptic activity via the specific binding of extended polySia chains on NCAM to FGF2. Oligo/polySia also functioned to impair the processing of proBDNF by plasmin via binding of oligo/polySia chains on NCAM. In addition, the polySia structure synthesized by mutated polysialyltransferase, ST8SIA2/STX(SNP7), which was previously identified from a schizophrenia patient, was impaired for these functions compared with polySia produced by normal ST8SIA2. Taken together, these data suggest that the protective effects of polySia toward FGF2 and proBDNF may be involved in the regulation of the concentrations of these neurologically active molecules.

    DOI: 10.1093/glycob/cwv049

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  21. Ganglioside contained in the neuronal tissue-enriched acidic protein of 22 kDa (NAP-22) fraction prepared from the detergent-resistant membrane microdomain of rat brain inhibits the phosphatase activity of calcineurin.

    Kobayashi Y, da Silva R, Kumanogoh H, Miyata S, Sato C, Kitajima K, Nakamura S, Morita M, Hayashi F, Maekawa S

    Journal of neuroscience research   Vol. 93 ( 9 ) page: 1462 - 70   2015.9

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Journal of Neuroscience Research  

    Neurons have well-developed membrane microdomains called "rafts" that are recovered as a detergent-resistant membrane microdomain fraction (DRM). Neuronal tissue-enriched acidic protein of 22 kDa (NAP-22) is one of the major protein components of neuronal DRM. To determine the cellular function of NAP-22, interacting proteins were screened with an immunoprecipitation assay, and calcineurin (CaN) was detected. Further studies with NAP-22 prepared from DRM and CaN expressed in bacteria showed the binding of these proteins and a dose-dependent inhibitory effect of the NAP-22 fraction on the phosphatase activity of CaN. On the other hand, NAP-22 expressed in bacteria showed low binding to CaN and a weak inhibitory effect on phosphatase activity. To solve this discrepancy, identification of a nonprotein component that modulates CaN activity in the DRM-derived NAP-22 fraction was attempted. After lyophilization, a lipid fraction was extracted with chloroform/methanol. The lipid fraction showed an inhibitory effect on CaN without NAP-22, and further fractionation of the extract with thin-layer chromatography showed the presence of several lipid bands having an inhibitory effect on CaN. The mobility of these bands coincided with that of authentic ganglioside (GM1a, GD1a, GD1b, and GT1b), and authentic ganglioside showed an inhibitory effect on CaN. Treatment of lipid with endoglycoceramidase, which degrades ganglioside to glycochain and ceramide, caused a diminution of the inhibitory effect. These results show that DRM-derived NAP-22 binds several lipids, including ganglioside, and that ganglioside inhibits the phosphatase activity of CaN.

    DOI: 10.1002/jnr.23599

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  22. Rapid Trimming of Cell Surface Polysialic Acid (PolySia) by Exovesicular Sialidase Triggers Release of Preexisting Surface Neurotrophin.

    Sumida M, Hane M, Yabe U, Shimoda Y, Pearce OM, Kiso M, Miyagi T, Sawada M, Varki A, Kitajima K, Sato C

    The Journal of biological chemistry   Vol. 290 ( 21 ) page: 13202 - 14   2015.5

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    As acidic glycocalyx on primary mouse microglial cells and a mouse microglial cell line Ra2, expression of polysialic acid (polySia/PSA), a polymer of the sialic acid Neu5Ac (N-acetylneuraminic acid), was demonstrated. PolySia is known to modulate cell adhesion, migration, and localization of neurotrophins mainly on neural cells. PolySia on Ra2 cells disappeared very rapidly after an inflammatory stimulus. Results of knockdown and inhibitor studies indicated that rapid surface clearance of polySia was achieved by secretion of endogenous sialidase Neu1 as an exovesicular component. Neu1-mediated polySia turnover was accompanied by the release of brain-derived neurotrophic factor normally retained by polySia molecules. Introduction of a single oxygen atom change into polySia by exogenous feeding of the non-neural sialic acid Neu5Gc (N-glycolylneuraminic acid) caused resistance to Neu1-induced polySia turnover and also inhibited the associated release of brain-derived neurotrophic factor. These results indicate the importance of rapid turnover of the poly-Sia glycocalyx by exovesicular sialidases in neurotrophin regulation.

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  23. Sulfatide-Hsp70 interaction promotes Hsp70 clustering and stabilizes binding to unfolded protein.

    Harada Y, Sato C, Kitajima K

    Biomolecules   Vol. 5 ( 2 ) page: 958 - 73   2015.5

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    The 70-kDa heat shock protein (Hsp70), one of the major stress-inducible molecular chaperones, is localized not only in the cytosol, but also in extracellular milieu in mammals. Hsp70 interacts with various cell surface glycolipids including sulfatide (3'-sulfogalactosphingolipid). However, the molecular mechanism, as well as the biological relevance, underlying the glycolipid-Hsp70 interaction is unknown. Here we report that sulfatide promotes Hsp70 oligomerization through the N-terminal ATPase domain, which stabilizes the binding of Hsp70 to unfolded protein in vitro. We find that the Hsp70 oligomer has apparent molecular masses ranging from 440 kDa to greater than 669 kDa. The C-terminal peptide-binding domain is dispensable for the sulfatide-induced oligomer formation. The oligomer formation is impaired in the presence of ATP, while the Hsp70 oligomer, once formed, is unable to bind to ATP. These results suggest that sulfatide locks Hsp70 in a high-affinity state to unfolded proteins by clustering the peptide-binding domain and blocking the binding to ATP that induces the dissociation of Hsp70 from protein substrates.

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  24. Discovery, primary, and crystal structures and capacitation-related properties of a prostate-derived heparin-binding protein WGA16 from boar sperm.

    Garénaux E, Kanagawa M, Tsuchiyama T, Hori K, Kanazawa T, Goshima A, Chiba M, Yasue H, Ikeda A, Yamaguchi Y, Sato C, Kitajima K

    The Journal of biological chemistry   Vol. 290 ( 9 ) page: 5484 - 501   2015.2

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    Mammalian sperm acquire fertility through a functional maturation process called capacitation, where sperm membrane molecules are drastically remodeled. In this study, we found that a wheat germ agglutinin (WGA)-reactive protein on lipid rafts, named WGA16, is removed from the sperm surface on capacitation. WGA16 is a prostate-derived seminal plasma protein that has never been reported and is deposited on the sperm surface in themalereproductive tract. BasedonproteinandcDNAsequences for purified WGA16, it is a homologue of human zymogen granule protein16(ZG16) belonging to the Jacalin-related lectin (JRL) family in crystal and primary structures. A glycan array shows that WGA16 binds heparin through a basic patch containing Lys-53/ Lys-73 residues but not the conventional lectin domain of the JRL family. WGA16 is glycosylated, contrary to other ZG16 members, and comparative mass spectrometry clearly shows its unique N-glycosylation profile among seminal plasma proteins. It has exposed GlcNAc and GalNAc residues without additional Gal residues. The GlcNAc/GalNAc residues can work as binding ligands foraspermsurface galactosyltransferase,whichactually galactosylatesWGA16in situ in the presence of UDP-Gal. Interestingly, surface removal of WGA16 is experimentally induced by either UDPGal or heparin. In the crystal structure, N-glycosylated sites and a potential heparin-binding site face opposite sides. This geography of two functional sites suggest that WGA16 is deposited on the sperm surface through interaction between its N-glycans and the surface galactosyltransferase, whereas its heparin-binding domain may be involvedinbindingto sulfatedglycosaminoglycansinthefemaletract, enabling removal ofWGA16from the sperm surface.

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  25. Polysialic acid: Biosynthesis, novel functions and applications

    Colley Karen J., Kitajima Ken, Sato Chihiro

    CRITICAL REVIEWS IN BIOCHEMISTRY AND MOLECULAR BIOLOGY   Vol. 49 ( 6 ) page: 498 - 532   2014.11

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    DOI: 10.3109/10409238.2014.976606

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  26. Crystal Structure of Anti-polysialic Acid Antibody Single Chain Fv Fragment Complexed with Octasialic Acid INSIGHT INTO THE BINDING PREFERENCE FOR POLYSIALIC ACID

    Nagae Masamichi, Ikeda Akemi, Hane Masaya, Hanashima Shinya, Kitajima Ken, Sato Chihiro, Yamaguchi Yoshiki

    JOURNAL OF BIOLOGICAL CHEMISTRY   Vol. 288 ( 47 ) page: 33784 - 33796   2013.11

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  27. Occurrence of free deaminoneuraminic acid (KDN)-containing complex-type N-glycans in human prostate cancers

    Yabu Masahiko, Korekane Hiroaki, Hatano Koji, Kaneda Yasufumi, Nonomura Norio, Sato Chihiro, Kitajima Ken, Miyamoto Yasuhide

    GLYCOBIOLOGY   Vol. 23 ( 6 ) page: 634 - 42   2013.6

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  28. Impact of structural aberrancy of polysialic acid and its synthetic enzyme ST8SIA2 in schizophrenia

    Sato Chihiro, Kitajima Ken

    FRONTIERS IN CELLULAR NEUROSCIENCE   Vol. 7 ( APR ) page: 61   2013

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  29. ポリシアル酸による神経機能の調節. 実験医学 31巻 10号 (増刊) pp. 26-33; 第三の生命鎖 Reviewed

    佐藤 ちひろ

    実験医学   Vol. 31   page: 26 - 33   2013

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  30. Sialome analysis of the cephalochordate Branchiostoma belcheri, a key organism for vertebrate evolution

    Guerardel Yann, Chang Lan-Yi, Fujita Akiko, Coddeville Bernadette, Maes Emmanuel, Sato Chihiro, Harduin-Lepers Anne, Kubokawa Kaoru, Kitajima Ken

    GLYCOBIOLOGY   Vol. 22 ( 4 ) page: 479 - 91   2012.4

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  31. 26 Combinatorial Synthesis of Ganglio-Series Ganglioside Epitopes and Their Biological Evaluation(Oral Presentation)

    Tanaka Hiroshi, Nishiura Yuji, Yasuda Yu, Sato Chihiro, Kitajima Ken, Takahashi Takashi

    Symposium on the Chemistry of Natural Products, symposium papers   Vol. 54 ( 0 ) page: 151 - 156   2012

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    Gangliosides are a family of glycolipids, which possess sialic acids that play important roles in biological events on cell surfaces. Ganglio-series gangliosides are composed of the tetrasaccharide, (Galβ(1,3)GalNAcβ(1,4)Galβ(1,4)Glc), which bears α(2,8) oligosialic acid units. Glycolipids that vary in number of sialic acids are known as the a-, b-, and c-series of gangliosides. Chemical synthesis of these di-/oligo-sialo-containing glycolipids and their derivatives would facilitate identification of their biological roles. However, the α(2,8) oligosialic acid unit and the compact and rigid 3,4 dibranched galactoside unit are difficult to prepare. Therefore, an effective method for the synthesis of a-, b-, and c-series of gangliosides is still needed. In this report, we describe combinatorial synthesis of ganglio-series ganglioside epitopes varying at the number of the sialic acids. The α(2,3) and α(2,8) sialylations were accomplished by use of 5N,4O-carbonyl and 7,8-O-isopropyliden, and 5N,4O-carbonyl- and 7,8-di-O-chloroacetyl-protected sialyl donors in good yields with excellent a-selectivity, respectively. The two sialyl donors enable synthesis of the di- and tri-sialylgalactosides by simple glycosylation and deprotection. We successfully prepared the ganglioside epitopes via direct construction of a compact, rigid, branched structure. We also reported on a competitive inhibiting activity of the soluble glycolipid library to binding solid-supported GD3 with Siglec-7.

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  32. Structural and functional impairments of polysialic acid (polySia)-neural cell adhesion molecule (NCAM) synthesized by a mutated polysialyltransferase of a schizophrenic patient

    Hane Masaya, Sumida Mizuki, Kitajima Ken, Sato Chihiro

    PURE AND APPLIED CHEMISTRY   Vol. 84 ( 9 ) page: 1895 - 1906   2012

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    DOI: 10.1351/PAC-CON-11-12-10

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  33. Co-expression of two distinct polysialic acids, alpha 2,8-and alpha 2,9-linked polymers of N-acetylneuraminic acid, in distinct glycoproteins and glycolipids in sea urchin sperm

    Miyata Shinji, Yamakawa Nao, Toriyama Masaru, Sato Chihiro, Kitajima Ken

    GLYCOBIOLOGY   Vol. 21 ( 12 ) page: 1596 - 605   2011.12

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  34. New Functions of Polysialic Acid and Its Relationship to Schizophrenia

    Sato Chihiro, Kitajima Ken

    Trends in Glycoscience and Glycotechnology   Vol. 23 ( 133 ) page: 221 - 238   2011.9

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    Polysialic acid (polySia), particularly α2,8-linked polyNeu5Ac (DP=8∼400), is a unique polymer that spatio-temporally modifies neural cell adhesion molecule (NCAM), predominantly in embryonic brains. PolySia is considered to function as a negative regulator of adhesion between cells, and between cells and extracellular matrix due to its extremely polyanionic nature and steric hindrance. Through its anti-adhesive property and spatio-temporal expression, polySia is involved in cell migration, neural outgrowth, axonal guidance, synaptic plasticity, and the development of normal neural circuits and neurogenesis. Due to improvements of polySia-detection methods, it has been shown that polySia expression persists in the hippocampus and olfactory bulb of adults, where neurogenesis is ongoing; however, the functions of polySia, particularly in adult brains, remain incompletely resolved. Recently, we demonstrated that polySia functions not only as an anti-adhesive molecule, but also as a reservoir for specific bioactive molecules, such as neurotrophic factors, neurotransmitters, and growth factors, which regulate neural function. In addition, studies have revealed that the enzymatic activity of STX, which is involved in the synthesis of polySia, derived from schizophrenic patient is low and that the product of the enzyme, polySia on NCAM is impaired with respect to both quantity and quality. Notably, impaired polySia-NCAM cannot effectively retain bioactive molecules, including BDNF, dopamine, and FGF2. Taken together, the impairment of polySia might influence the retention function of polySia and lead to the development of psychiatric disorders, such as schizophrenia, which are reported to express either lower or elevated amounts of polySia compared with normal brains.

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  35. Structural and Functional Impairments of Polysialic Acid by a Mutated Polysialyltransferase Found in Schizophrenia

    Isomura Ryo, Kitajima Ken, Sato Chihiro

    JOURNAL OF BIOLOGICAL CHEMISTRY   Vol. 286 ( 24 ) page: 21535 - 45   2011.6

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    DOI: 10.1074/jbc.M111.221143

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  36. IgG2 dominancy and carbohydrate recognition specificity of C3H/He mouse antibodies directed to cross-reactive carbohydrate determinants (CCDs) bearing beta-(1,2)-xylose and alpha-(1,3)-fucose

    Hino Shingo, Umeda Fumiko, Inumaru Shigeki, Aoki Naohito, Sato Chihiro, Okajima Tetsuya, Nadano Daita, Matsuda Tsukasa

    IMMUNOLOGY LETTERS   Vol. 133 ( 1 ) page: 28 - 34   2010.9

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    DOI: 10.1016/j.imlet.2010.06.007

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  37. Extensive enrichment of N-glycolylneuraminic acid in extracellular sialoglycoproteins abundantly synthesized and secreted by human cancer cells

    Inoue Sadako, Sato Chihiro, Kitajima Ken

    GLYCOBIOLOGY   Vol. 20 ( 6 ) page: 752 - 62   2010.6

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    DOI: 10.1093/glycob/cwq030

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  38. Glycans and schizophrenia

      Vol. 61 ( 2 ) page: 160 - 166   2010.4

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    DOI: 10.11477/mf.2425100979

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  39. MEASUREMENT OF GLYCAN-BASED INTERACTIONS BY FRONTAL AFFINITY CHROMATOGRAPHY AND SURFACE PLASMON RESONANCE

    Sato Chihiro, Yamakawa Nao, Kitajima Ken

    METHODS IN ENZYMOLOGY, VOL 478: GLYCOMICS   Vol. 478 ( C ) page: 219 - 32   2010

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    DOI: 10.1016/S0076-6879(10)78010-1

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  40. 糖鎖と統合失調症

    佐藤 ちひろ

    生体の科学 61     page: 160 - 166   2010

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  41. Complex Formation of a Brain-Derived Neurotrophic Factor and Glycosaminoglycans

    KANATO Yukihiro, ONO Sayaka, KITAJIMA Ken, SATO Chihiro

    Bioscience, Biotechnology, and Biochemistry   Vol. 73 ( 12 ) page: 2735 - 41   2009.12

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    Glycosaminoglycans (GAGs), large anionic glycopolymers, are the glycan portion of proteoglycans and are important components of the extracellular matrix. Recently we reported that polysialic acid, a polyanionic glycopolymer specific to the brain, binds neurotrophic factors to form a large complex. It is not clear whether GAGs also bind neurotrophic factors to form a large complex. In the present study, we demonstrate that a brain-derived neurotrophic factor (BDNF) dimer directly binds GAGs other than chondroitin and hyaluronic acid to form a large complex. Neurotrophin-3 showed similar GAG binding properties. Furthermore, BDNF, after forming a large complex with GAG, bound to the BDNF receptors tropomyosin-related kinase (Trk) B and p75 neurotrophin receptor (NTR). These findings suggest that GAGs function to produce a reservoir of BDNF and other neurotrophic factors, and may serve to regulate their local concentrations on the cell surface.

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  42. Developmental regulation of oligosialylation in zebrafish

    Chang Lan-Yi, Harduin-Lepers Anne, Kitajima Ken, Sato Chihiro, Huang Chang-Jen, Khoo Kay-Hooi, Guerardel Yann

    GLYCOCONJUGATE JOURNAL   Vol. 26 ( 3 ) page: 247 - 61   2009.4

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  43. Membrane microdomains from early gastrula embryos of medaka, Oryzias latipes, are a platform of E-cadherin- and carbohydrate-mediated cell-cell interactions during epiboly

    Adachi Tomoko, Sato Chihiro, Kishi Yasunori, Totani Kazuhide, Murata Takeomi, Usui Taichi, Kitajima Ken

    GLYCOCONJUGATE JOURNAL   Vol. 26 ( 3 ) page: 285 - 99   2009.4

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    DOI: 10.1007/s10719-008-9184-y

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  44. 3P-066 Docking simulation and biochemical analyses of sialylated glycan recognition of sialic acid binding Ig-like lectin (Siglec)-7(Protein:Function,The 47th Annual Meeting of the Biophysical Society of Japan)

    Yamakawa Nao, Kosaki Hitomi, Crocker Paul, Vergoten Gerard, Sato Chihiro, Kitajima Ken

    Seibutsu Butsuri   Vol. 49 ( supplement ) page: S162   2009

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    DOI: 10.2142/biophys.49.s162_2

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  45. Direct binding of polysialic acid to a brain-derived neurotrophic factor depends on the degree of polymerization

    Kanato Yukihiro, Kitajima Ken, Sato Chihiro

    GLYCOBIOLOGY   Vol. 18 ( 12 ) page: 1044 - 53   2008.12

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  46. ポリシアル酸構造による細胞シグナルの調節機構

    佐藤 ちひろ

    北島健蛋白質核酸酵素 53     page: 1577 - 1583   2008

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  47. ポドシアル酸構造による細胞シグナルの調節機構.

    佐藤 ちひろ

    蛋白質核酸酵素 53     page: 1577 - 1583   2008

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  48. Development of sensitive chemical and immunochemical methods for detecting sulfated sialic acids and their application to glycoconjugates from sea urchin sperm and eggs

    Yamakawa Nao, Sato Chihiro, Miyata Shinji, Maehashi Eri, Toriyama Masaru, Sato Noriko, Furuhata Kimio, Kitajima Ken

    BIOCHIMIE   Vol. 89 ( 11 ) page: 1396 - 408   2007.11

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  49. The rainbow trout CMP-sialic acid synthetase utilises a nuclear localization signal different from that identified in the mouse enzyme

    Tiralongo Joe, Fujita Akiko, Sato Chihiro, Kitajima Ken, Lehmann Friederike, Oschlies Melanie, Gerardy-Schahn Rita, Muenster-Kuehnel Anja K.

    GLYCOBIOLOGY   Vol. 17 ( 9 ) page: 945 - 54   2007.9

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  50. Membrane microdomain formation is crucial in epiboly during gastrulation of medaka

    Adachi Tomoko, Sato Chihiro, Kitajima Ken

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   Vol. 358 ( 3 ) page: 848 - 53   2007.7

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    DOI: 10.1016/j.bbrc.2007.04.197

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  51. Hypoxia-enhanced expression of free deaminoneuraminic acid in human cancer cells

    Go Shinji, Sato Chihiro, Yin Jun, Kannagi Reiji, Kitajima Ken

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   Vol. 357 ( 2 ) page: 537 - 42   2007.6

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    DOI: 10.1016/j.bbrc.2007.03.181

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  52. Identification of the nuclear export signals that regulate the intracellular localization of the mouse CMP-sialic acid synthetase

    Fujita Akiko, Sato Chihiro, Kitajima Ken

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   Vol. 355 ( 1 ) page: 174 - 80   2007.3

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  53. Identification of an Inflammation-inducible Serum Protein Recognized by Anti-disialic Acid Antibodies as Carbonic Anhydrase II

    YASUKAWA Zenta, SATO Chihiro, KITAJIMA Ken

    Journal of Biochemistry   Vol. 141 ( 3 ) page: 429 - 441   2007.3

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  54. Glycobiology of Polysialic Acids on Sea Urchin Gametes

    Miyata Shinji, Sato Chihiro, Kitajima Ken

    Trends in Glycoscience and Glycotechnology   Vol. 19 ( 106 ) page: 85 - 98   2007.3

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    Naturally occurring polysialic acids (polySias), a unique homopolymer of sialic acid (Sia) residues, have a large structural diversity, primarily arising from the diversities in Sia components as well as in the intersialyl linkages. The α2,8-linked polySia on the neural cell adhesion molecules (NCAM) is the most extensively studied and recognized as a regulator of cell-cell interactions during development and differentiation in vertebrate brain. However, little is known about the occurrence and functions of polySia other than the α2,8-linked polySia on NCAM. Recently, it has been shown that polySia occurs in nature more frequently than previously recognized, as analytical methods to detect polySia structures have been greatly improved. Using these methods, two different types of polySia, α2,8- and α2,9-linked polySia, have been discovered on the sea urchin sperm, although the α2,5<i>O<sub>glycolyl</sub></i>-linked polySia was already known in sea urchin egg. This is the first example of the co-localization of polySia with different intersialyl linkages in the same cell. The α2,9-linked polySia-containing glycoprotein of sea urchin sperm flagella was identified and named “flagellasialin”. The α2,9-linked polySia on flagellasialin is suggested as a regulator of intracellular Ca<sup>2+</sup> and sperm motility. In addition, the α2,5<i>O<sub>glycolyl</sub></i>-linked polySia has recently been shown to increase intracellular pH and potentiate acrosome reaction of sea urchin sperm. These results indicate that these polySia structures in sea urchin gametes have new functions other than the negative regulator for the NCAM-mediated cell adhesion. In this review, we summarize the recent advances in structural and functional studies of polySia in sea urchin gametes.

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  55. Complex formation of 70-kDa heat shock protein with acidic glycolipids and phospholipids

    Harada Yoichiro, Sato Chihiro, Kitajima Ken

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   Vol. 353 ( 3 ) page: 655 - 60   2007.2

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  56. Involvement of the α2,8-Polysialyltransferases II/STX and IV/PST in the Biosynthesis of Polysialic Acid Chains on the O-Linked Glycoproteins in Rainbow Trout Ovary

    ASAHINA Shinji, SATO Chihiro, MATSUNO Midori, MATSUDA Tsukasa, COLLEY Karen, KITAJIMA Ken

    Journal of Biochemistry   Vol. 140 ( 5 ) page: 687 - 701   2006.11

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  57. Involvement of the alpha 2,8-polysialyltransferases II/STX and IV/PST in the biosynthesis of polysialic acid chains on the O-linked glycoproteins in rainbow trout ovary

    Asahina Shinji, Sato Chihiro, Matsuno Midori, Matsuda Tsukasa, Colley Karen, Kitajima Ken

    JOURNAL OF BIOCHEMISTRY   Vol. 140 ( 5 ) page: 687 - 701   2006.11

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  58. Oral ingestion of mannose alters the expression level of deaminoneuraminic acid (KDN) in mouse organs

    Go Shinji, Sato Chihiro, Furuhata Kimio, Kitajima Ken

    GLYCOCONJUGATE JOURNAL   Vol. 23 ( 5-6 ) page: 411 - 21   2006.7

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  59. Weaning-induced expression of a milk-fat globule protein, MFG-E8, in mouse mammary glands, as demonstrated by the analyses of its mRNA, protein and phosphatidylserine-binding activity

    Nakatani H, Aoki N, Nakagawa Y, Jin-No S, Aoyama K, Oshima K, Ohira S, Sato C, Nadano D, Matsuda T

    BIOCHEMICAL JOURNAL   Vol. 395 ( 1 ) page: 21 - 30   2006.4

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  60. Hypoxic culture induces expression of sialin, a sialic acid transporter, and cancer-associated gangliosides containing non-human sialic acid on human cancer cells

    Yin J, Hashimoto A, Izawa M, Miyazaki K, Chen GY, Takematsu H, Kozutsumi Y, Suzuki A, Furuhata K, Cheng FL, Lin CH, Sato C, Kitajima K, Kannagi R

    CANCER RESEARCH   Vol. 66 ( 6 ) page: 2937 - 45   2006.3

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  61. AN N-TERMINAL 130 KDA FRAGMENT OF A FOURTH SEA URCHIN SPERM RECEPTOR FOR EGG JELLY MODULE PROTEIN, REJ4, IS SECRETED DURING ACROSOME REACTION(Developmental Biology,Abstracts of papers presented at the 76^<th> Annual Meeting of the Zoological Society of Japan) :

    MiyataShinji, SatoChihiro, ToriyamaMasaru, KitajamaKen

    Zoological science   Vol. 22 ( 12 ) page: 1456   2005.12

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  62. CAPACITATION-INDUCED CHANGES OF THE SURFACE DISTRIBUTION OF SIALOGLYCOTOPES ON PIG SPERM(Developmental Biology,Abstracts of papers presented at the 76^<th> Annual Meeting of the Zoological Society of Japan) :

    XueLian, MiyataShinji, SatoChihiro, KitajimaKen

    Zoological science   Vol. 22 ( 12 ) page: 1456   2005.12

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  63. 5.糖鎖と発生、4)ポリシアル酸

    佐藤 ちひろ

    遺伝子医学(糖鎖と病気)MOOK 3     page: 186 - 191   2005

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  64. A MAJOR GANGLIOSIDE FROM HEMICENTROTUS PULCHERRIMUS SPERM SPECIFICALLY INTERACTS WITH PHOSPHOLIPIDS(Developmental Biology,Abstracts of papers presented at the 75^<th> Annual Meeting of the Zoological Society of Japan) :

    YamakawaNao, SatoChihiro, KitajimaKen

    Zoological science   Vol. 21 ( 12 ) page: 1292   2004.12

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  65. CELL GROWTH STIMULATING ACTIVITY OF THE PEPTIDES DERIVED FROM CORTICAL ALVEOLUS GLYCOPROTEINS OF FISH EGG (HYOSOPHORIN)(Developmental Biology,Abstracts of papers presented at the 75^<th> Annual Meeting of the Zoological Society of Japan) :

    MaedaEri, SatoChihiro, KitajimaKen

    Zoological science   Vol. 21 ( 12 ) page: 1292   2004.12

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  66. THE 130 KDA GLYCOPROTEIN IS SECRETED FROM SPERM DURING ACROSOME REACTION OF SEA URCHIN, HEMICENTROTUS PULCHERRIMUS(Developmental Biology,Abstracts of papers presented at the 75^<th> Annual Meeting of the Zoological Society of Japan) :

    MiyataShinji, SatoChihiro, ToriyamaMasaru, HirohashiNoritaka, VacquierVictor, KitajimaKen

    Zoological science   Vol. 21 ( 12 ) page: 1292   2004.12

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  67. A newly identified zona pellucida glycoprotein, ZPD, and dimeric ZP1 of chicken egg envelope are involved in sperm activation on sperm-egg interaction

    Okumura H, Kohno Y, Iwata Y, Mori H, Aoki N, Sato C, Kitajima K, Nadano D, Matsuda T

    BIOCHEMICAL JOURNAL   Vol. 384 ( Pt 1 ) page: 191 - 9   2004.11

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    DOI: 10.1042/BJ20040299

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  68. Specificity of human trans-sialidase as probed with gangliosides

    Nikonova EY, Tertov VV, Sato C, Kitajima K, Bovin NV

    BIOORGANIC & MEDICINAL CHEMISTRY LETTERS   Vol. 14 ( 20 ) page: 5161 - 5164   2004.10

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    DOI: 10.1016/j.bmcl.2004.07.058

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  69. Preface for the Special Issue Entitled "New Waves in Sialobioscience"

    SATO Chihiro

    Trends in glycoscience and glycotechnology   Vol. 16 ( 91 ) page: 291 - 292   2004.9

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  70. Preface for the Special Issue Entitled “New Waves in Sialobioscience”

    Sato Chihiro

    Trends in Glycoscience and Glycotechnology   Vol. 16 ( 91 ) page: 291 - 292   2004.9

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    DOI: 10.4052/tigg.16.291

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  71. Chain Length Diversity of Sialic Acids and Its Biological Significance

    Sato Chihiro

    Trends in Glycoscience and Glycotechnology   Vol. 16 ( 91 ) page: 331 - 344   2004.9

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    Sialic acids are commonly present as monosialyl residues at the non-reducing terminal end of glycoconjugates. In some cases, α2→8 linked di/oligosialic acid chains with DP 2 or 3 sialic acid residues are common structural units of gangliosides, and involved in various biological processes, such as cell adhesion, cell differentiation, signal transduction, and surface expression of stage specific antigen. In contrast, little attention has been paid to the occurrence and functions of such short sialyl di/oligomers on glycoproteins, while polysialic acid (DP≥8) in embryonic NCAM has been extensively studied as a regulator of cell adhesion in neurogenesis. As analytical methods to detect di/oligosialic acid structures have been improved, several glycoproteins containing di/oligo/polysialic acid chains have been identified. It is thus hypothesized that these di/oligosialic acid residues on glycoproteins may have similar important functions in common with those proposed for the gangliosides or may have new functions. Currently, several studies showing the importance of di/oligosialic acid-containing glycoproteins have emerged. In this review, recent advances in such studies of di/oligosialic acid residues on glycoproteins, including analytical methods, occurrence, biosynthetic pathways, and functions, are described.

    DOI: 10.4052/tigg.16.331

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  72. Developmental Expression of a Sialyltransferase Responsible for Sialylation of Cortical Alveolus Glycoprotein during Oogenesis in Rainbow Trout (Oncorhynchus mykiss)

    ASAHINA Shinji, SATO Chihiro, KITAJIMA Ken

    Journal of Biochemistry   Vol. 136 ( 2 ) page: 189 - 198   2004.8

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  73. Developmental Expression of a Sialyltransferase Responsible for Sialylation of Cortical Alveolus Glycoprotein during Oogenesis in Rainbow Trout (<i>Oncorhynchus mykiss</i>)

    Asahina Shinji, Sato Chihiro, Kitajima Ken

    The Journal of Biochemistry   Vol. 136 ( 2 ) page: 189 - 98   2004.8

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    Polysialoglycoprotein (PSGP) is a major cortical alveolus glycoprotein of rainbow trout eggs that is characterized by the attachment of a polysialic acid structure to its <i>O</i>-glycan chains. It has been demonstrated that the polysialic acid structure is synthesized by at least three sialyltransferases mostly localized in cortical alveoli. Here we have cloned a cDNA encoding the sialyltransferase, designated rtST6GaINAc, responsible for the transfer of the first sialic acid residue onto the <i>O</i>-glycan chain of PSGP. This enzyme belongs to the vertebrate ST6GaINAc II family, and is strongly expressed in ovaries. Of those <i>O</i>-glycoproteins tested as substrates, asialo-PSGP is the best substrate. These results indicate that rtST6GalNAc is the enzyme responsible for the sialylation of PSGP during oogenesis. Furthermore, the rtST6GalNAc mRNA is expressed throughout oogenesis, is down-regulated at the late yolk vesicle stage (May), and then up-regulated during vitellogenesis (until August). This developmental profile is highly similar to that of STL2, a cortical alveolus lectin, while it is quite different from that of PSGP, which is extensively expressed at the yolk vesicle stage and down-regulated at later stages. Thus, not all cortical alveolus components are transcribed concomitantly. This is the first description of a developmental change in the transcription of a glycosyltransferase during oogenesis.

    DOI: 10.1093/jb/mvh106

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  74. alpha-C-mannosyltryptophan is not recognized by conventional mannose-binding lectins

    Nishikawa T, Kajii S, Sato C, Yasukawa Z, Kitajima K, Isobe M

    BIOORGANIC & MEDICINAL CHEMISTRY   Vol. 12 ( 9 ) page: 2343 - 8   2004.5

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    DOI: 10.1016/j.bmc.2004.02.009

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  75. ジシアル酸・オリゴシアル酸・ポリシアル酸の生物学的機能

    佐藤 ちひろ

    Functional Glycomics 3     page: 40 - 47   2004

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  76. FORMATION OF MEMBRANE MICRODOMAINS IS SIGNIFICANT IN DEVELOPING EMBRYOS OF MEDAKA(Developmental Biology,Abstracts of papers presented at the 74^<th> Annual Meeting of the Zoological Society of Japan) :

    AdachiTomoko, SatoChihiro, KitajimaKen

    Zoological science   Vol. 20 ( 12 ) page: 1570   2003.12

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  77. FUNCTIONS OF THE COMMON GLYCOTOPES OF GLYCOLIPIDS AND GLYCOPROTEINS IN THE MEMBRANE MICRODOMAIN OF SEA URCHIN SPERM(Developmental Biology)(Proceedings of the Seventy-Third Annual Meeting of the Zoological Society of Japan) :

    MiyataShinji, MaehashiEri, OhtaKaoru, SatoChihiro, MatsudaTsukasa, KitajimaKen

    Zoological science   Vol. 19 ( 12 ) page: 1443   2002.12

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  78. Neuronal differentiation-dependent expression of the disialic acid epitope on CD166 and its involvement in neurite formation in neuro2A cells

    Sato C, Matsuda T, Katajima K

    JOURNAL OF BIOLOGICAL CHEMISTRY   Vol. 277 ( 47 ) page: 45299 - 305   2002.11

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    DOI: 10.1074/jbc.M206046200

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  79. ジ・オリゴシアル酸構造の生物学的機能

    佐藤 ちひろ, 北島 健

      Vol. 6   page: A4J   2002.6

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    DOI: 10.32285/glycoforum.06a4j

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  80. Function of Di/oligosialic Acid Structure

    Sato Chihiro, Kitajima Ken

    Glycoforum   Vol. 6   page: A4   2002.6

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    DOI: 10.32285/glycoforum.06a4

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  81. Occurrence of Oligosialic Acids on Integrin α<inf>5</inf> Subunit and Their Involvement in Cell Adhesion to Fibronectin

    Nadanaka S., Sato C., Kitajima K., Katagiri K., Irie S., Yamagata T.

    Journal of Biological Chemistry   Vol. 276 ( 36 ) page: 33657 - 33664   2001.9

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    Integrin α5β1, a major fibronectin receptor, functions in a wide variety of biological phenomena. We have found that α2-8-linked oligosialic acids with 5 ≤ degree of polymerization (DP) ≤ 7 occur on integrin α5 subunit of the human melanoma cell line G361. The integrin α5 subunit immunoprecipitated with anti-integrin α5 antibody reacted with the monoclonal antibody 12E3, which recognizes oligo/polysialic acid with DP ≥ 5 but not with the polyclonal antibody H.46 recognizing oligo/polysialic acid with DP ≥ 8. The occurrence of oligosialic acids was further demonstrated by fluorometric C7/C9 analysis on the immunopurified integrin α5 subunit. Oligosialic acids were also found in the α5 subunit of several other human cells such as foreskin fibroblast and chronic erythroleukemia K562 cells. These results suggest the ubiquitous modification with unique oligosialic acids occurs on the α 5 subunit of integrin α5β1. The adhesion of human melanoma G361 cells to fibronectin was mainly mediated by integrin α5β1. Treatment of cells with sialidase from Arthrobacter ureafaciens cleaving α2-3-, α2-6-, and α2-8-linked sialic acids inhibited adhesion to fibronectin. On the other hand, N-acetylneuraminidase II, which cleaves α2-3 and α2-6 but not α2-8 linkages, showed no inhibitory activity. After the loss of oligosialic acids, integrin α5β1 failed to bind to fibronectin-conjugated Sepharose, indicating that the oligosialic acid on the α5 subunit of integrin α5β 1 plays important roles in cell adhesion to fibronectin.

    DOI: 10.1074/jbc.M011100200

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  82. Identification and Adipocyte Differentiation-dependent Expression of the Unique Disialic Acid Residue in an Adipose Tissue-specific Glycoprotein, Adipo Q

    Sato C.

    Journal of Biological Chemistry   Vol. 276 ( 31 ) page: 28849 - 28856   2001.8

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    DOI: 10.1074/jbc.M104148200

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  83. The Occurrence and Significance of Sialic Acids in Insects.

    Schauer Roland

    Trends in Glycoscience and Glycotechnology   Vol. 13 ( 73 ) page: 507 - 517   2001

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    Sialic acids, mainly <i>N</i>-acetylneuraminic acid, have been found during the development of a few insect species. In <i>Drosophila melanogaster</i> and the cicada <i>Philaenus spumarius</i> we detected polysialic acids, using lectins, antibodies, and various methods for the isolation and analysis of the monosaccharide subunits, in areas of neuronal development and in Malpighian tubules, respectively. However, sialic acids were not found in the adult animals of these species. Although the expression of these acidic sugars has unequivocally been demonstrated <i>in vivo</i>, their occurrence in insect cell cultures was, in most cases, not clearly demonstrated. The biosynthesis of sialic acids in cell cultures would be of great biotechnological significance, since the production of recombinant glycoproteins with mammalian type, complex, sialylated <i>N</i>-glycans using the baculovirus expression system would be of great benefit. From the studies dedicated to this problem it appears that the engineering of such glycans may only be possible by the expression of exogenous genes in insect cells. Several genes encoding sialic acid-metabolizing enzymes have therefore been transfected. The work on insect cells shows that sialic acids are not only restricted to the Deuterostomia branch of the animal kingdom but also occur in some Protostomia species, as in insects. This throws new light on the evolution of this acidic sugar.

    DOI: 10.4052/tigg.13.507

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  84. Co-localization of receptor and transducer proteins in the glycosphingolipid-enriched, low density, detergent-insoluble membrane fraction of sea urchin sperm

    Ohta K.

    Glycoconjugate Journal   Vol. 17 ( 3-4 ) page: 205 - 214   2000.12

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    DOI: 10.1023/A:1026589223811

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  85. Frequent occurrence of pre-existing α2→8-linked disialic and oligosialic acids with chain lengths up to 7 Sia residues in mammalian brain glycoproteins. Prevalence revealed by highly sensitive chemical methods and anti-di-, oligo-, and poly-Sia antibodies specific for defined chain lengths

    Sato C., Fukuoka H., Ohta K., Matsuda T., Koshino R., Kobayashi K., Troy F.A., Kitajima K.

    Journal of Biological Chemistry   Vol. 275 ( 20 ) page: 15422 - 15431   2000.5

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    The pre-existence of α2→8-linked disialic acid (di-Sia) and oligosialic acid (oligo-Sia) structures with up to 7 Sia residues was shown to occur on a large number of brain glycoproteins, including neural cell adhesion molecules (N-CAMs), by two highly sensitive chemical methods (Sato, C., Inoue, S., Matsuda, T., and Kitajima, K. (1998) Anal. Biochem. 261, 191- 197; Sato, C., Inoue, S., Matsuda, T., and Kitajima, K. (1999) Anal Biochem. 266, 102-109). This unexpected finding was also confirmed using a newly developed antibody prepared using a copolymer of α2→8-linked N- acetylneuraminyl p-vinylbenzylamide and acrylamide as an immunogen and known antibodies whose immunospecificities were determined to be di- and oligo-Sia residues with defined chain lengths. The major significance of the new finding that di- and oligo-Sia chains exist on a large number of brain glycoproteins is 2-fold. First, it reveals a surprising diversity in the number and M(r) of proteins distinct from N-CAM that are covalently modified by these short sialyl glycotopes. Second, it suggests that synthesis of di- and/or oligo-Sia units may be catalyzed by α2→8-sialyltransferase(s) that are distinct from the known polysialyltransferases, STX and PST, which are partially responsible for polysialylation of N-CAM.

    DOI: 10.1074/jbc.275.20.15422

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  86. Lipid raft on gametic cells as a functional domain for sperm-egg interaction coupled with signal transduction

    Ohta K.

    Zygote   Vol. 8 ( SUPPL. ) page: .   2000

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    DOI: 10.1017/s0967199400130321

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  87. O-Acetylated Sialic Acids. Their Detection, Biological Significance and Alteration in Diseases.

    Sinha D., Chatterjee M., Mandal C.

    Trends in Glycoscience and Glycotechnology   Vol. 12 ( 63 ) page: 17 - 33   2000

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    Sialic acids are acidic, 9-carbon carboxylated monosaccharides, typically expressed on the outermost end of the sugar chains of membrane and secreted glycoconjugates. This particular location provides it accessibility reflected in its regulation of a multitude of cellular and molecular interactions. Amongst the diverse structural variations that occur, the commonest is <i>O</i>-acetylation at C-<sub>4, 7, 8</sub> and<sub>9</sub> carbon positions. This diversity in <i>O</i>-acetylated sialic acid (<i>O</i>-AcSA) derivatives is attributed to their linkage to the underlying sugar chain, the carbon position that gets <i>O</i>-acetylated and nature of the glycoconjugates to which they are attached. Steady refinement of analytical techniques has allowed for identification of <i>O</i>-AcSA derivatives in several diseases leading to a shift in focus towards understanding the biological importance of this modification. The present review deals primarily with the recently evolved information regarding detection of altered <i>O</i>-acetylation in various pathophysiological conditions and their biological relevance. Particular attention is focused on two diverse diseases, namely, Acute Lymphoblastic Leukemia (ALL) and Visceral Leishmaniasis (VL) in which 9-<i>O</i> acetylated sialoglycoconjugates (9-<i>O</i>AcSGs) have proved to be effective biomarkers for monitoring their clinical status.

    DOI: 10.4052/tigg.12.17

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  88. Glycobiology of Di- and Oligosialyl Glycotopes.

    Sato Chihiro, Kitajima Ken

    Trends in Glycoscience and Glycotechnology   Vol. 11 ( 62 ) page: 371 - 390   1999.11

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    α2→8-Linked di- and oligosialic acid (diSia and oligoSia) chains with DP 2, 3 Sia residues are known to be common structural units of gangliosides, and to be involved in various biological processes, such as cell adhesion, cell differentiation, signal transduction, and surface expression of stage specific antigen. In contrast, little attention has been paid to the occurrence and functions of such short sialyl oligomers on glycoproteins. However, it has recently been shown that glycoproteins containing di- and oligoSia groups occur in nature more frequently than was ever recognized, as analytical methods to detect di- and oligoSia structures have improved. It is thus hypothesized that these di- and oligoSia moieties on glycoproteins may have similar important functions in common with those proposed for the gangliosides. In this review, we describe the recent advances in the study of di- and oligoSia residues on glycoproteins, including analytical methods, occurrence, functions, and biosynthetic pathways.

    DOI: 10.4052/tigg.11.371

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  89. Cholesterol-dependent localization of NAP-22 on a neuronal membrane microdomain (Raft)

    Maekawa S.

    Journal of Biological Chemistry   Vol. 274 ( 30 ) page: 21369 - 21374   1999.7

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    DOI: 10.1074/jbc.274.30.21369

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  90. Isolation and characterization of low density detergent-insoluble membrane (LD-DIM) fraction from sea urchin sperm

    Ohta K.

    Biochemical and Biophysical Research Communications   Vol. 258 ( 3 ) page: 616 - 623   1999.5

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    DOI: 10.1006/bbrc.1999.0686

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  91. A 42-kDa glycoprotein from chicken egg-envelope, an avian homolog of the ZPC family glycoproteins in mammalian zona pellucida. Its first identification, cDNA cloning and granulosa cell-specific expression

    Takeuchi Y.

    European Journal of Biochemistry   Vol. 260 ( 3 ) page: 736 - 742   1999.3

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    DOI: 10.1046/j.1432-1327.1999.00203.x

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  92. Fluorescent-assisted detection of oligosialyl units in glycoconjugates

    Sato C.

    Analytical Biochemistry   Vol. 266 ( 1 ) page: 102 - 109   1999.1

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    DOI: 10.1006/abio.1998.2921

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  93. Development of a highly sensitive chemical method for detecting α2→8- linked oligo/polysialic acid residues in glycoproteins blotted on the membrane

    Sato C., Inoue S., Matsuda T., Kitajima K.

    Analytical Biochemistry   Vol. 261 ( 2 ) page: 191 - 197   1998.8

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    A highly sensitive chemical method for detecting α2→8-linked oligo/polysialic acid (oligo/polySia) chains was developed, including (i) periodate oxidation, reduction with sodium borohydride, and subsequent acid hydrolysis, giving rise to C7 analogues and intact C9 compounds from nonreducing terminal and internal sialic acid residues, respectively; (ii) fluorescent labeling of these C7 and C9 compounds with 1,2-diamino-4,5- methylenedioxybenzene (DMB); and (iii) quantitation of these DMB derivatives on fluorometric high-performance liquid chromatography. As little as 1 ng of internal sialic acid residues of oligo/polySia chains, the existence of which indicates the presence of oligo/polySia structure, was detectable by this method. This fluorometric C7/C9 analysis was successfully applied to glycoproteins blotted onto a slit of polyvinylidene fluoride membranes and suggested the presence of some novel oligoSia-containing glycoproteins in pig embryonic brains.

    DOI: 10.1006/abio.1998.2718

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  94. Identification of oligo-N-glycolylneuraminic acid residues in mammal- derived glycoproteins by a newly developed immunochemical reagent and biochemical methods

    Sato C.

    Journal of Biological Chemistry   Vol. 273 ( 5 ) page: 2575 - 2582   1998.1

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    DOI: 10.1074/jbc.273.5.2575

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  95. Occurrence of terminal α2 → 8-linked disialylated poly-N-acetyllactosamine chains with Le(X) and I antigenic glycotopes in tetraantennary arms of an N-linked glycoprotein isolated from rainbow trout ovarian fluid

    Funakoshi Y., Taguchi T., Sato C., Kitajima K., Inoue S., Morris H.R., Dell A., Inoue Y.

    Glycobiology   Vol. 7 ( 2 ) page: 195 - 205   1997.3

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    The Pronase digestion of a 54K glycoprotein present in ovarian fluid of rainbow trout yielded a major glycopeptide. Carbohydrate compositional analysis revealed that this glycopeptide was likely to possess a single large N-glycan chain having low molecular weight oligomers of N-acetylneuraminic acid (oligoNeu5Ac). Structural studies of this glycopeptide revealed novel α2 → 8-linked disialylated poly-N-acetyllactosamine chains with Le(X) and I antigenic determinants on the N-linked tetraantennary core glycan. In our recent studies (Kitazume, S., Kitajima, K., Inoue, S., Inoue, Y. and Troy, F.A. (1994) J. Biol. Chem. 269, 10330-10340) we presented evidence that synthesis of α2 → 8-linked polysialic acid (polySia) chains is a two-step process in which chain initiation is catalyzed by an α2 → 8-sialyltransferase (α2 → 8-ST; initiase) that catalyzes synthesis of the first Sia α2 → 8-linkage, forming the disialic acid (diSia) unit, Siaα2 → 8-Siaα2 → 6-Gal-.Chain polymerization is then postulated to be catalyzed by a second enzyme, an α2 → 8-polyST ('polymerase') that converts the diSia units to polySia chains. The present structural studies leading to the discovery of α2 → 8-linked disialylated units that terminate poly-N-acetyllactosamine chains in an N-linked glycoprotein is further evidence in support of our hypothesis that more than one sialyltransferase activity is required for polySia chain synthesis and polymerization.

    DOI: 10.1093/glycob/7.2.195

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  96. Polysialylation and disease. Reviewed International journal

    Chihiro Sato, Ken Kitajima

    Molecular aspects of medicine   Vol. 79   page: 100892 - 100892   2021.6

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    Polysialic acid (polySia, PSA) is a unique constituent of the glycocalyx on the surface of bacterial and vertebrate cells. In vertebrates, its biosynthesis is highly regulated, not only in quantity and quality, but also in time and location, which allows polySia to be involved in various important biological phenomena. Therefore, impairments in the expression and structure of polySia sometimes relate to diseases, such as schizophrenia, bipolar disorder, and cancer. Some bacteria express polySia as a tool for protecting themselves from the host immune system during invasion. PolySia is proven to be a biosafe material; polySia, as well as polySia-recognizing molecules, are key therapeutic agents. This review first comprehensive outlines the occurrence, features, biosynthesis, and functions of polySia and subsequently focuses on the related diseases.

    DOI: 10.1016/j.mam.2020.100892

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  97. Evolutionary conservation of human ketodeoxynonulosonic acid production is independent of sialoglycan biosynthesis. Reviewed International coauthorship International journal

    Kunio Kawanishi, Sudeshna Saha, Sandra Diaz, Michael Vaill, Aniruddha Sasmal, Shoib S Siddiqui, Biswa Choudhury, Kumar Sharma, Xi Chen, Ian C Schoenhofen, Chihiro Sato, Ken Kitajima, Hudson H Freeze, Anja Münster-Kühnel, Ajit Varki

    The Journal of clinical investigation   Vol. 131 ( 5 )   2021.3

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    Human metabolic incorporation of nonhuman sialic acid (Sia) N-glycolylneuraminic acid into endogenous glycans generates inflammation via preexisting antibodies, which likely contributes to red meat-induced atherosclerosis acceleration. Exploring whether this mechanism affects atherosclerosis in end-stage renal disease (ESRD), we instead found serum accumulation of 2-keto-3-deoxy-d-glycero-d-galacto-2-nonulosonic acid (Kdn), a Sia prominently expressed in cold-blooded vertebrates. In patients with ESRD, levels of the Kdn precursor mannose also increased, but within a normal range. Mannose ingestion by healthy volunteers raised the levels of urinary mannose and Kdn. Kdn production pathways remained conserved in mammals but were diminished by an M42T substitution in a key biosynthetic enzyme, N-acetylneuraminate synthase. Remarkably, reversion to the ancestral methionine then occurred independently in 2 lineages, including humans. However, mammalian glycan databases contain no Kdn-glycans. We hypothesize that the potential toxicity of excess mannose in mammals is partly buffered by conversion to free Kdn. Thus, mammals probably conserve Kdn biosynthesis and modulate it in a lineage-specific manner, not for glycosylation, but to control physiological mannose intermediates and metabolites. However, human cells can be forced to express Kdn-glycans via genetic mutations enhancing Kdn utilization, or by transfection with fish enzymes producing cytidine monophosphate-Kdn (CMP-Kdn). Antibodies against Kdn-glycans occur in pooled human immunoglobulins. Pathological conditions that elevate Kdn levels could therefore result in antibody-mediated inflammatory pathologies.

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  98. Exploring the Impact of Ketodeoxynonulosonic Acid in Host-Pathogen Interactions Using Uptake and Surface Display by Nontypeable Haemophilus influenzae. International coauthorship International journal

    Sudeshna Saha, Alison Coady, Aniruddha Sasmal, Kunio Kawanishi, Biswa Choudhury, Hai Yu, Ricardo U Sorensen, Jaime Inostroza, Ian C Schoenhofen, Xi Chen, Anja Münster-Kühnel, Chihiro Sato, Ken Kitajima, Sanjay Ram, Victor Nizet, Ajit Varki

    mBio   Vol. 12 ( 1 ) page: 1 - 24   2021.1

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    Surface expression of the common vertebrate sialic acid (Sia) N-acetylneuraminic acid (Neu5Ac) by commensal and pathogenic microbes appears structurally to represent "molecular mimicry" of host sialoglycans, facilitating multiple mechanisms of host immune evasion. In contrast, ketodeoxynonulosonic acid (Kdn) is a more ancestral Sia also present in prokaryotic glycoconjugates that are structurally quite distinct from vertebrate sialoglycans. We detected human antibodies against Kdn-terminated glycans, and sialoglycan microarray studies found these anti-Kdn antibodies to be directed against Kdn-sialoglycans structurally similar to those on human cell surface Neu5Ac-sialoglycans. Anti-Kdn-glycan antibodies appear during infancy in a pattern similar to those generated following incorporation of the nonhuman Sia N-glycolylneuraminic acid (Neu5Gc) onto the surface of nontypeable Haemophilus influenzae (NTHi), a human commensal and opportunistic pathogen. NTHi grown in the presence of free Kdn took up and incorporated the Sia into its lipooligosaccharide (LOS). Surface display of the Kdn within NTHi LOS blunted several virulence attributes of the pathogen, including Neu5Ac-mediated resistance to complement and whole blood killing, complement C3 deposition, IgM binding, and engagement of Siglec-9. Upper airway administration of Kdn reduced NTHi infection in human-like Cmah null (Neu5Gc-deficient) mice that express a Neu5Ac-rich sialome. We propose a mechanism for the induction of anti-Kdn antibodies in humans, suggesting that Kdn could be a natural and/or therapeutic "Trojan horse" that impairs colonization and virulence phenotypes of free Neu5Ac-assimilating human pathogens.IMPORTANCE All cells in vertebrates are coated with a dense array of glycans often capped with sugars called sialic acids. Sialic acids have many functions, including serving as a signal for recognition of "self" cells by the immune system, thereby guiding an appropriate immune response against foreign "nonself" and/or damaged cells. Several pathogenic bacteria have evolved mechanisms to cloak themselves with sialic acids and evade immune responses. Here we explore a type of sialic acid called "Kdn" (ketodeoxynonulosonic acid) that has not received much attention in the past and compare and contrast how it interacts with the immune system. Our results show potential for the use of Kdn as a natural intervention against pathogenic bacteria that take up and coat themselves with external sialic acid from the environment.

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  99. The conserved arginine residue in all siglecs is essential for Siglec-7 binding to sialic acid. Reviewed International journal

    Atsushi Yoshimura, Rina Hatanaka, Hiroshi Tanaka, Ken Kitajima, Chihiro Sato

    Biochemical and biophysical research communications   Vol. 534   page: 1069 - 1075   2021.1

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    Siglecs are sialic acid (Sia)-binding immunoglobulin-like lectins; the majority of Siglecs functions as transmembrane receptors on the immune cells via Sia residues. Recently, a new Sia binding site in Siglec-7, termed site 2, where arginine (R) 67 was critical, was identified by computational modeling and biochemical analyses, relative to the primary Sia binding site, termed site 1, containing critical R124. Here, the presence of a new essential R94 residue, which is completely conserved among all identified Siglecs, was demonstrated. A mutation of R94 residue in Siglec-7 led to the disappearance of the Sia binding property, similar to a site 1 mutation (R124A). R94 is close to R67 in site 2, and site 2 mutations at either of them abolished the ligand-binding properties to both gangliosides and glycoproteins. These data suggest that, in addition to site 1, the conserved R residue among Siglecs in site 2 is another functional site.

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  100. Comparative Studies of Polysialic Acids Derived from Five Different Vertebrate Brains. Reviewed International journal

    Yi Yang, Ryo Murai, Yuka Takahashi, Airi Mori, Masaya Hane, Ken Kitajima, Chihiro Sato

    International journal of molecular sciences   Vol. 21 ( 22 ) page: 1 - 22   2020.11

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    Polysialic acid (polySia/PSA) is a linear homopolymer of sialic acid (Sia) that primarily modifies the neural cell adhesion molecule (NCAM) in mammalian brains. PolySia-NCAM not only displays an anti-adhesive function due to the hydration effect, but also possesses a molecule-retaining function via a direct binding to neurologically active molecules. The quality and quantity of polySia determine the function of polySia-NCAM and are considered to be profoundly related to the maintenance of normal brain functions. In this study, to compare the structures of polySia-NCAM in brains of five different vertebrates (mammals, birds, reptiles, amphibians, and fish), we adopted newly developed combinational methods for the analyses. The results revealed that the structural features of polySia considerably varied among different species. Interestingly, mice, as a mammal, possess eminently distinct types of polySia, in both quality and quantity, compared with those possessed by other animals. Thus, the mouse polySia is of larger quantities, of longer and more diverse chain lengths, and of a larger molecular size with higher negative charge, compared with polySia of other species. These properties might enable more advanced brain function. Additionally, it is suggested that the polySia/Sia ratio, which likely reflects the complexity of brain function, can be used as a new promising index to evaluate the intelligence of different vertebrate brains.

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  101. Combinational Analyses with Multiple Methods Reveal the Existence of Several Forms of Polysialylated Neural Cell Adhesion Molecule in Mouse Developing Brains. Reviewed International journal

    Airi Mori, Yi Yang, Yuka Takahashi, Masaya Hane, Ken Kitajima, Chihiro Sato

    International journal of molecular sciences   Vol. 21 ( 16 ) page: 1 - 20   2020.8

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    Polysialic acid (polySia/PSA) is an anionic glycan polymer of sialic acid, and it mostly modifies the neural cell adhesion molecule (NCAM) in mammalian brains. Quality and quantity of the polySia of the polySia-NCAM is spatio-temporally regulated in normal brain development and functions, and their impairments are reported to be related to diseases, such as psychiatric disorders and cancers. Therefore, precise understanding of the state of polySia-NCAM structure would lead to the diagnosis of diseases for which their suitable evaluation methods are necessary. In this study, to develop these evaluation methods, structures of polySia-NCAM from mouse brains at six different developmental stages were analyzed by several conventional and newly developed methods. Integrated results of these experiments clearly demonstrated the existence of different types of polySia-NCAMs in developing brains. In addition, combinational analyses were shown to be useful for precise understanding of the quantity and quality of polySia, which can provide criteria for the diagnosis of diseases.

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  102. Discovery of a new sialic acid binding region that regulates Siglec-7. Reviewed International coauthorship International journal

    Nao Yamakawa, Yu Yasuda, Atsushi Yoshimura, Ami Goshima, Paul R Crocker, Gérard Vergoten, Yuji Nishiura, Takashi Takahashi, Shinya Hanashima, Kana Matsumoto, Yoshiki Yamaguchi, Hiroshi Tanaka, Ken Kitajima, Chihiro Sato

    Scientific reports   Vol. 10 ( 1 ) page: 8647 - 8647   2020.5

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    Siglec-7 is a human CD33-like siglec, and is localised predominantly on human natural killer (NK) cells and monocytes. Siglec-7 is considered to function as an immunoreceptor in a sialic acid-dependent manner. However, the underlying mechanisms linking sialic acid-binding and function remain unknown. Here, to gain new insights into the ligand-binding properties of Siglec-7, we carried out in silico analysis and site-directed mutagenesis, and found a new sialic acid-binding region (site 2 containing R67) in addition to the well-known primary ligand-binding region (site 1 containing R124). This was supported by equilibrium dialysis, STD-NMR experiments, and inhibition analysis of GD3-binding toward Siglec-7 using synthetic sialoglycoconjugates and a comprehensive set of ganglioside-based glycoconjugates. Our results suggest that the two ligand-binding sites are potentially controlled by each other due to the flexible conformation of the C-C' loop of Siglec-7.

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  103. Effect of expression alteration in flanking genes on phenotypes of St8sia2-deficient mice. Reviewed International journal

    Keisuke Ikegami, Kazumasa Saigoh, Atsuko Fujioka, Mamoru Nagano, Ken Kitajima, Chihiro Sato, Satoru Masubuchi, Susumu Kusunoki, Yasufumi Shigeyoshi

    Scientific reports   Vol. 9 ( 1 ) page: 13634 - 13634   2019.9

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    ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 2 (ST8SIA2) synthesizes polysialic acid (PSA), which is essential for brain development. Although previous studies reported that St8sia2-deficient mice that have a mixed 129 and C57BL/6 (B6) genetic background showed mild and variable phenotypes, the reasons for this remain unknown. We hypothesized that this phenotypic difference is caused by diversity in the expression or function of flanking genes of St8sia2. A genomic polymorphism and gene expression analysis in the flanking region revealed reduced expression of insulin-like growth factor 1 receptor (Igf1r) on the B6 background than on that of the 129 strain. This observation, along with the finding that administration of an IGF1R agonist during pregnancy increased litter size, suggests that the decreased expression of Igf1r associated with ST8SIA2 deficiency caused lethality. This study demonstrates the importance of gene expression level in the flanking regions of a targeted null allele having an effect on phenotype.

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  104. Functional roles of ST8SIA3-mediated sialylation of striatal dopamine D<sub>2</sub> and adenosine A<sub>2A</sub> receptors. Reviewed International coauthorship

    Lin CY, Lai HL, Chen HM, Siew JJ, Hsiao CT, Chang HC, Liao KS, Tsai SC, Wu CY, Kitajima K, Sato C, Khoo KH, Chern Y

    Translational psychiatry   Vol. 9 ( 1 ) page: 209   2019.8

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  105. Acute stress-induced change in polysialic acid levels mediated by sialidase in mouse brain. Reviewed

    Abe C, Yi Y, Hane M, Kitajima K, Sato C

    Scientific reports   Vol. 9 ( 1 ) page: 9950   2019.7

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  106. N-linked mannose glycoconjugates on shrimp thrombospondin, pmTSP-II, and their involvement in the sperm acrosome reaction. Reviewed International coauthorship

    Timklay W, Magerd S, Sato C, Somrit M, Watthammawut A, Senarai T, Weerachatyanukul W, Kitajima K, Asuvapongpatana S

    Molecular reproduction and development   Vol. 86 ( 4 ) page: 440 - 449   2019.4

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  107. Sialic Acids in Neurology. Reviewed

    Sato C, Kitajima K

    Advances in carbohydrate chemistry and biochemistry   Vol. 76   page: 1 - 64   2019

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    DOI: 10.1016/bs.accb.2018.09.003

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  108. Structural Biology of Glycans Reviewed

    Sato C, Kato K, Yamaguchi Y, Kohda D, Kato R, Suzuki GN K, Kikuchi K, Hirai G, Kizuka Y, Tanaka K, Nakashima Y, Setou M

    Glycoscience: Basic Science to Applications     page: 35 - 63   2019

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  109. Preparation and Properties of a Polycyclic p-Quinodimethane with Two Oxygen Bridges and its Radical Cation in Comparison with the Isomeric o-Quinodimethane.

    Sato C, Suzuki S, Okada K, Kozaki M

    Chemistry, an Asian journal   Vol. 13 ( 23 ) page: 3729 - 3736   2018.12

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  110. Systems glycomics of adult zebrafish identifies organ-specific sialylation and glycosylation patterns. Reviewed International journal

    Yamakawa N, Vanbeselaere J, Chang LY, Yu SY, Ducrocq L, Harduin-Lepers A, Kurata J, Aoki-Kinoshita KF, Sato C, Khoo KH, Kitajima K, Guerardel Y

    Nature communications   Vol. 9 ( 1 ) page: 4647 - 4647   2018.11

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    The emergence of zebrafish Danio rerio as a versatile model organism provides the unique opportunity to monitor the functions of glycosylation throughout vertebrate embryogenesis, providing insights into human diseases caused by glycosylation defects. Using a combination of chemical modifications, enzymatic digestion and mass spectrometry analyses, we establish here the precise glycomic profiles of eight individual zebrafish organs and demonstrate that the protein glycosylation and glycosphingolipid expression patterns exhibits exquisite specificity. Concomitant expression screening of a wide array of enzymes involved in the synthesis and transfer of sialic acids shows that the presence of organ-specific sialylation motifs correlates with the localized activity of the corresponding glycan biosynthesis pathways. These findings provide a basis for the rational design of zebrafish lines expressing desired glycosylation profiles.

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  111. Mental disorders and an acidic glycan-from the perspective of polysialic acid (PSA/polySia) and the synthesizing enzyme, ST8SIA2. Reviewed

    Sato C, Hane M

    Glycoconjugate journal   Vol. 35 ( 4 ) page: 353 - 373   2018.8

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  112. 酸性糖鎖ポリシアル酸の新機能の発見とその応用展開 Reviewed

    佐藤 ちひろ

    化学と生物   Vol. 56   page: 422 - 431   2018

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  113. Positive selection on schizophrenia-associated ST8SIA2 gene in post-glacial Asia. Reviewed

    Fujito NT, Satta Y, Hane M, Matsui A, Yashima K, Kitajima K, Sato C, Takahata N, Hayakawa T

    PloS one   Vol. 13 ( 7 ) page: e0200278   2018

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    A number of loci are associated with highly heritable schizophrenia and the prevalence of this mental illness has had considerable negative fitness effects on human populations. Here we focused on one particular schizophrenia-associated gene that encodes a sialyl-transferase (ST8SIA2) and is expressed preferentially in the brain with the level being largely determined by three SNPs in the promoter region. It is suggested that the expression level of the ST8SIA2 gene is a genetic determinant of schizophrenia risk, and we found that a geographically differentiated non-risk SNP type (CGC-type) has significantly reduced promoter activity. A newly developed method for detecting ongoing positive selection was applied to the ST8SIA2 genomic region with the identification of an unambiguous sweep signal in a rather restricted region of 18 kb length surrounding the promoter. We also found that while the CGC-type emerged in anatomically modern humans in Africa over 100 thousand years ago, it has increased its frequency in Asia only during the past 20-30 thousand years. These findings support that the positive selection is driven by psychosocial stress due to changing social environments since around the last glacial maximum, and raise a possibility that schizophrenia extensively emerged during the Upper Paleolithic and Neolithic era.

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  114. Different properties of polysialic acids synthesized by the polysialyltransferases ST8SIA2 and ST8SIA4

    Airi Mori, Masaya Hane, Yuki Niimi, Ken Kitajima, Chihiro Sato

    GLYCOBIOLOGY   Vol. 27 ( 9 ) page: 834 - 846   2017.9

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    Polysialic acid (polySia) is mainly found as a modification of neural cell adhesion molecule (NCAM) in whole embryonic brains, as well as restricted areas of adult vertebrate brains, including the hippocampus. PolySia shows not only repulsive effects on NCAM-involved cell-cell interactions due to its bulky and hydrated properties, but also attractive effects on the interaction with neurologically active molecules, which exerts a reservoir function. Two different polysialyltransferases, ST8SIA2 and ST8SIA4, are involved in the synthesis of polySia chains; however, to date, the differences of the properties between polySia chains synthesized by these two enzymes remain unknown. In this study, to clarify this point, we first prepared polySia-NCAMs from HEK293 cells stably expressing ST8SIA4 and ST8SIA2, or ST8SIA2 (SNP-7), a mutant ST8SIA2 derived from a schizophrenia patient. The conventional sensitive chemical and immunological characterizations showed that the quantity and quality (structural features) of polySia are not so much different between ST8SIA4-and ST8SIA2-synthesized ones, apart from those of ST8SIA2 (SNP-7). Then, we assessed the homophilic and heterophilic interactions mediated by polySia-NCAM by adopting a surface plasmon resonance measurement as an in vitro analytical method. Our novel findings are as follows: (i) the ST8SIA2-and ST8SIA4-synthesized polySia-NCAMs exhibited different attractive and repulsive effects than each other; (ii) both polySia-and oligoSia-NCAMs synthesized by ST8SIA2 were able to bind polySia-NCAMs; (iii) the polySia-NCAM synthesized by a ST8SIA2 (SNP-7) showed markedly altered attractive and repulsive properties. Collectively, polySia-NCAM is suggested to simultaneously possess both attractive and repulsive properties that are highly regulated by the two polysialyltransferases.

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  115. Chemical Synthesis and Evaluation of a Disialic Acid-Containing Dextran Polymer as an Inhibitor for the Interaction between Siglec 7 and Its Ligand

    Sho Yamaguchi, Atsushi Yoshimura, Yu Yasuda, Airi Mori, Hiroshi Tanaka, Takashi Takahashi, Ken Kitajima, Chihiro Sato

    CHEMBIOCHEM   Vol. 18 ( 13 ) page: 1194 - 1203   2017.7

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    A new sialic acid (Sia)-containing glycopolymer-a fluorescent probe with high-density disialic acid (diSia) on the surface of polysaccharide dextran (diSia-Dex)-was synthesized as a key molecule to regulate the Sia recognition lectins, Siglecs, that are involved in the immune system. According to our original methods, diSia was synthesized by alpha-selective sialylation, and a dextran template possessing terminal acetylenes and amino groups was prepared. A diSia and a fluorescent molecule were subsequently introduced to surface-modified dextran by Huisgen reaction and amidation, respectively. The modulatory activity of Siglec7 was evaluated by using synthetic probes. DiSia-Dex showed high binding avidity toward Siglec7, with a KD value of 5.87 x 10(-10) M, and a high inhibitory activity for the interaction between Siglec7 and a ligand (GD3), with a IC50 value of 1.0 nM. Notably, diSia-Dex was able to release Siglec7 from the pre-existing Siglec7-GD3 complex, possibly due to its unique properties of a slow dissociation rate and a high association rate. Together, these data show that diSia-Dex can be widely applicable as a modulator of Siglec7 functions.

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  116. Alpha-2 macroglobulin as a region-specific secretory protein in male reproductive tract, and its dynamics during sperm transit toward the female spermatheca in the blue crab

    Thanyaporn Senarai, Rapeepun Vanichviriyakit, Shinji Miyata, Chihiro Sato, Prapee Sretarugsa, Wattana Weerachatyanukul, Ken Kitajima

    MOLECULAR REPRODUCTION AND DEVELOPMENT   Vol. 84 ( 7 ) page: 585 - 595   2017.7

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    A 250-kDa protein was isolated from fluid in the middle spermatic duct (MSD) of the blue crab (Portunus pelagicus). N-terminal and partial amino acid sequences revealed that this MSD-specific protein is highly similar to the plasma-enriched protein Alpha-2 macroglobulin (alpha 2M). The P. pelagicus ortholog (Pp alpha 2M) is a large glycoprotein possessing mannose and N-acetylglucosamine residues. Pp alpha 2M mRNA was detected in the spermatic duct, androgenic gland, and hematopoietic tissue, whereas the protein was primarily observed in the apical cytoplasm of MSD epithelium and in the matrix of the acrosome of MSD sperm; distally within spermatic duct, Pp alpha 2M was lost from the sperm membrane but remained in the sperm acrosome. These results suggest that Pp alpha 2M is expressed and glycosylated in the epithelium of spermatic ducts, secreted into MSD fluid, taken up by sperm in the MSD, and removed from the surface of sperm during its transit towards the female spermatheca. Given that Pp alpha 2M also exhibits protease inhibitor activity, we hypothesize that acrosome localized Pp alpha 2M may suppress premature acrosome reaction during post-testicular sperm maturation in this crab.

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  117. Chlorpromazine Increases the Expression of Polysialic Acid (PolySia) in Human Neuroblastoma Cells and Mouse Prefrontal Cortex

    Chikara Abe, Saki Nishimura, Airi Mori, Yuki Niimi, Yi Yang, Masaya Hane, Ken Kitajima, Chihiro Sato

    INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES   Vol. 18 ( 6 )   2017.5

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    The neural cell adhesion molecule (NCAM) is modified by polysialic acid (polySia or PSA) in embryonic brains. In adult brains, polySia modification of NCAM is only observed in restricted areas where neural plasticity, remodeling of neural connections, or neural generation is ongoing although the amount of NCAM remains unchanged. Impairments of the polySia-expression and several single nucleotide polymorphisms (SNPs) of the polysialyltransferase (polyST) ST8SIA2 gene are reported to be associated with schizophrenia and bipolar disorder. Chlorpromazine (CPZ) is well-known as an agent for treating schizophrenia, and our hypothesis is that CPZ may affect the polySia expression or the gene expression of polySTs or NCAM. To test this hypothesis, we analyzed the effects of CPZ on the expression of polySia-NCAM on human neuroblastoma cell line, IMR-32 cells, by immunochemical and chemical methods. Interestingly, the cell surface expression of polySia, especially those with lower chain lengths, was significantly increased on the CPZ-treated cells, while mRNAs for polySTs and NCAM, and the amounts of total polySia-NCAM remained unchanged. The addition of brefeldin A, an inhibitor of endocytosis, suppressed the CPZ-induced cell surface polySia expression. In addition, polySia-NCAM was also observed in the vesicle compartment inside the cell. All these data suggest that the level of cell surface expression of polySia in IMR-32 is highly regulated and that CPZ changes the rate of the recycling of polySia-NCAM, leading to the up-regulation of polySia-NCAM on the cell surface. We also analyzed the effect of CPZ on polySia-expression in various brain regions in adult mice and found that CPZ only influenced the total amounts of polySia-NCAM in prefrontal cortex. These results suggest a brain-region-specific effect of CPZ on the expression of total polySia in mouse brain. Collectively, anti-schizophrenia agent CPZ consistently up-regulates the expression polySia at both cellular and animal levels.

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  118. Altered expression of ganglioside GM3 molecular species and a potential regulatory role during myoblast differentiation

    Shinji Go, Shiori Go, Lucas Veillon, Maria Grazia Ciampa, Laura Mauri, Chihiro Sato, Ken Kitajima, Alessandro Prinetti, Sandro Sonnino, Jin-ichi Inokuchi

    JOURNAL OF BIOLOGICAL CHEMISTRY   Vol. 292 ( 17 ) page: 7040 - 7051   2017.4

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    Gangliosides (sialic acid-containing glycosphingolipids) help regulate many important biological processes, including cell proliferation, signal transduction, and differentiation, via formation of functional microdomains in plasma membranes. The structural diversity of gangliosides arises from both the ceramide moiety and glycan portion. Recently, differing molecular species of a given ganglioside are suggested to have distinct biological properties and regulate specific and distinct biological events. Elucidation of the function of each molecular species is important and will provide new insights into ganglioside biology. Gangliosides are also suggested to be involved in skeletal muscle differentiation; however, the differential roles of ganglioside molecular species remain unclear. Here we describe striking changes in quantity and quality of gangliosides (particularly GM3) during differentiation of mouse C2C12 myoblast cells and key roles played by distinct GM3 molecular species at each step of the process.

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  119. Diverse subcellular localizations of the insect CMP-sialic acid synthetases

    Wu Di, Akiko Fujita, Kayo Hamaguchi, Philippe Delannoy, Chihiro Sato, Ken Kitajima

    GLYCOBIOLOGY   Vol. 27 ( 4 ) page: 329 - 341   2017.4

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    The occurrence and biological importance of sialic acid (Sia) and its metabolic enzymes in insects have been studied using Drosophila melanogaster. The most prominent feature of D. melanogaster CMP-Sia synthetase (DmCSS) is its Golgi-localization, contrasted with nuclear localization of vertebrate CSSs. However, it remains unclear if the Golgi-localization is common to other insect CSSs and why it happens. To answer these questions, Aedes aegypti (mosquito) CSS (AaCSS) and Tribolium castaneum (beetle) CSS (TcCSS) were cloned and characterized for their activity and subcellular localization. Our new findings show: (1) AaCSS and TcCSS share a common overall structure with DmCSS in terms of evolutionarily conserved motifs and the absence of the C-terminal domain typical to vertebrate CSSs; (2) when expressed in mammalian and insect cells, AaCSS and TcCSS showed in vivo and in vitro CSS activities, similar to DmCSS. In contrast, when expressed in bacteria, they lacked CSS activity because the N-terminal hydrophobic region appeared to induce protein aggregation; (3) when expressed in Drosophila S2 cells, AaCSS and TcCSS were predominantly localized in the ER, but not in the Golgi. Surprisingly, DmCSS was mainly secreted into the culture medium, although partially detected in Golgi. Consistent with these results, the N-terminal hydrophobic regions of AaCSS and TcCSS functioned as a signal peptide to render them soluble in the ER, while the N-terminus of DmCSS functioned as a membrane-spanning region of type II transmembrane proteins whose cytosolic KLK sequence functioned as an ER export signal. Accordingly, the differential subcellular localization of insect CSSs are distinctively more diverse than previously recognized.

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  120. Synthesis of end-functionalized glycopolymers containing α(2,8) disialic acids via π-allyl nickel catalyzed coordinating polymerization and their interaction with Siglec-7. Reviewed

    Ohira S, Yasuda Y, Tomita I, Kitajima K, Takahashi T, Sato C, Tanaka H.

    Chem Commun (Camb).   Vol. 53 ( 3 ) page: 553-556.   2017.1

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  121. Releasing Mechanism of Neurotrophic Factors via Polysialic Acid

    C. Sato

    NEUROTROPHINS   Vol. 104   page: 89 - 112   2017

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    Neurotrophins are well-characterized neurologically active molecules in the central nervous system. The regulation of these signaling molecules, which are involved in cell growth, differentiation, and survival, is critical for normal brain function. Among the different types of neurotrophins, brain-derived neurotrophic factor (BDNF) is involved in various brain functions, including memory consolidation, synaptic plasticity, and adult neurogenesis, and is therefore a key molecule for understanding comprehensive brain function and neurodevelopmental and psychiatric diseases. The concentration of BDNF in body fluid is highly related to several neurodevelopmental and psychiatric diseases, including Alzheimer's diseases, depression, schizophrenia, and bipolar disorder. In the present review, the mechanisms by which BDNF is released from secretory vesicles are reviewed, with a particular focus on the recently described glycan-mediated release. In addition, the impact of glycan-mediated BDNF release on psychiatric disorders is also discussed.

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  122. ポリシアル酸転移酵素遺伝子ST8SIA2と精神疾患の関わり Reviewed

    羽根正弥, 北島 健, 佐藤 ちひろ

    生化学   Vol. 89   page: 634 - 643   2017

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  123. Diverse subcellular localizations of the insect CMP-sialic acid synthetases. Reviewed

    Di W, Fujita A, Hamaguchi K, Delannoy P, Sato C, Kitajima K.

    Glycobiology.     2016.12

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    DOI: doi: 10.1093/glycob/cww128.

  124. The Out of Africa and mental disorders: Adaptation with reduction in STX promoter activity

    Fujito Naoko, Satta Yoko, Hane Masaya, Matsui Atsushi, Yashima Kenta, Kitajima Ken, Sato Chihiro, Takahata Naoyuki, Hayakawa Toshiyuki

    GENES & GENETIC SYSTEMS   Vol. 91 ( 6 ) page: 343 - 343   2016.12

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  125. A Uniquely Human Evolutionary Change in ST8Sia-II Impacts Enzyme Stability and Polysialic Acid Function

    Vaill Michael, Hane Masaya, Naito-Matsui Yuko, Diaz Sandra, Davies Leela, Kitajima Ken, Sato Chihiro, Varki Ajit

    GLYCOBIOLOGY   Vol. 26 ( 12 ) page: 1423 - 1423   2016.12

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  126. Effects of intronic single nucleotide polymorphisms (iSNPs) of a polysialyltransferase, ST8SIA2 gene found in psychiatric disorders on its gene products Reviewed

    Masaya Hane, Ken Kitajima, Chihiro Sato

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   Vol. 478 ( 3 ) page: 1123 - 9   2016.9

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    Polysialic acid (polySia) is a linear homopolymer of sialic acid and mainly modifies neural cell adhesion molecule. PolySia plays important roles in synapse formation, learning and memory, social behavior and is associated with several diseases. Gene analyses of one of the biosynthetic enzymes for polySia, ST8SIA2, have revealed that several SNPs and genetic variations in the ST8SIA2 gene are associated with several psychiatric disorders; however, the mechanisms underlying these associations remain unknown. Here, we analyzed the effects of two iSNPs of ST8SIA2, rs2168351 and rs3784730, which are associated with bipolar disorder and autism spectrum disorder, respectively, on the expression of mRNA, ST8SIA2 and its final product, polySia in mouse neuroblastoma and human adenocarcinoma cell lines. We found that both iSNPs affected the expression of pre-mRNA and mRNA of ST8SIA2, and altered the cellular levels of ST8SIA2 and polySia. Taken together, these results indicate that impairment of the regulated expression of ST8SIA2 and the resulting downstream effects on gene products by these two iSNPs contribute to the development of these psychiatric disorders. (C) 2016 Elsevier Inc. All rights reserved.

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  127. Effects of intronic single nucleotide polymorphisms (iSNPs) of a polysialyltransferase, ST8SIA2 gene found in psychiatric disorders on its gene products. Reviewed

    Hane M, Kitajima K, Sato C.

    Biochem Biophys Res Commun.   Vol. 478 ( 3 ) page: 1123-9.   2016.9

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    DOI: doi: 10.1016/j.bbrc.2016.08.079.

  128. Relationship between ST8SIA2, polysialic acid and its binding molecules, and psychiatric disorders

    Sato C.

    Biochimica et Biophysica Acta - General Subjects   Vol. 1860 ( 8 ) page: 1739 - 1752   2016.8

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    DOI: 10.1016/j.bbagen.2016.04.015

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  129. Relationship between ST8SIA2, polysialic acid and its binding molecules, and psychiatric disorders. Invited Reviewed

    Sato C, Hane M, Kitajima K.

    Biochim Biophys Acta.   Vol. 1860 ( 8 ) page: 1739-52   2016.8

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    DOI: doi: 10.1016/j.bbagen.2016.04.015.

  130. Relationship between ST8SIA2, polysialic acid and its binding molecules, and psychiatric disorders.

    Sato C, Hane M, Kitajima K

    Biochimica et biophysica acta   Vol. 1860 ( 8 ) page: 1739 - 52   2016.8

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    DOI: 10.1016/j.bbagen.2016.04.015

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  131. Relationship between ST8SIA2, polysialic acid and its binding molecules, and psychiatric disorders. Reviewed

    Sato C, Hane M, Kitajima K

    Biochimica et biophysica acta   Vol. 1860 ( 8 ) page: 1739 - 1752   2016.8

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  132. Relationship between ST8SIA2, polysialic acid and its binding molecules, and psychiatric disorders Reviewed

    Chihiro Sato, Masaya Hane, Ken Kitajima

    BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS   Vol. 1860 ( 8 ) page: 1739 - 1752   2016.8

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    Polysialic acid (polySia, PSA) is a unique and functionally important glycan, particularly in vertebrate brains. It is involved in higher brain functions such as learning, memory, and social behaviors. Recently, an association between several genetic variations and single nucleotide polymorphisms (SNPs) of ST8SIA2/STX, one of two polysialyltransferase genes in vertebrates, and psychiatric disorders, such as schizophrenia (SZ), bipolar disorder (BD), and autism spectrum disorder (ASD), was reported based on candidate gene approaches and genome-wide studies among normal and mental disorder patients. It is of critical importance to determine if the reported mutations and SNPs in ST8SIA2 lead to impairments of the structure and function of polySia, which is the final product of ST8SIA2. To date, however, only a few such forward-directed studies have been conducted. In addition, the molecular mechanisms underlying polySia-involved brain functions remain unknown, although polySia was shown to have an anti-adhesive effect. In this report, we review the relationships between psychiatric disorders and polySia and/or ST8SIA2, and describe a new function of polySia as a regulator of neurologically active molecules, such as brain-derived neurotrophic factor (BDNF) and dopamine, which are deeply involved in psychiatric disorders. This article is part of a Special Issue entitled "Glycans in personalised medicine" Guest Editor: Professor Gordan Lauc. (C) 2016 Elsevier B.V. All rights reserved.

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  133. Relationship between ST8SIA2, polysialic acid and its binding molecules, and psychiatric disorders

    Sato Chihiro, Hane Masaya, Kitajima Ken

    BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS   Vol. 1860 ( 8 ) page: 1739 - 1752   2016.8

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    DOI: 10.1016/j.bbagen.2016.04.015

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  134. The AMOR Arabinogalactan Sugar Chain Induces Pollen-Tube Competency to Respond to Ovular Guidance. Reviewed International journal

    Akane G Mizukami, Rie Inatsugi, Jiao Jiao, Toshihisa Kotake, Keiko Kuwata, Kento Ootani, Satohiro Okuda, Subramanian Sankaranarayanan, Yoshikatsu Sato, Daisuke Maruyama, Hiroaki Iwai, Estelle Garénaux, Chihiro Sato, Ken Kitajima, Yoichi Tsumuraya, Hitoshi Mori, Junichiro Yamaguchi, Kenichiro Itami, Narie Sasaki, Tetsuya Higashiyama

    Current biology : CB   Vol. 26 ( 8 ) page: 1091 - 7   2016.4

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    Precise directional control of pollen-tube growth by pistil tissue is critical for successful fertilization of flowering plants [1-3]. Ovular attractant peptides, which are secreted from two synergid cells on the side of the egg cell, have been identified [4-6]. Emerging evidence suggests that the ovular directional cue is not sufficient for successful guidance but that competency control by the pistil is critical for the response of pollen tubes to the attraction signal [1, 3, 7]. However, the female molecule for this competency induction has not been reported. Here we report that ovular methyl-glucuronosyl arabinogalactan (AMOR) induces competency of the pollen tube to respond to ovular attractant LURE peptides in Torenia fournieri. We developed a method for assaying the response capability of a pollen tube by micromanipulating an ovule. Using this method, we showed that pollen tubes growing through a cut style acquired a response capability in the medium by receiving a sufficient amount of a factor derived from mature ovules of Torenia. This factor, named AMOR, was identified as an arabinogalactan polysaccharide, the terminal 4-O-methyl-glucuronosyl residue of which was necessary for its activity. Moreover, a chemically synthesized disaccharide, the β isomer of methyl-glucuronosyl galactose (4-Me-GlcA-β-(1→6)-Gal), showed AMOR activity. No specific sugar-chain structure of plant extracellular matrix has been identified as a bioactive molecule involved in intercellular communication. We suggest that the AMOR sugar chain in the ovary renders the pollen tube competent to the chemotropic response prior to final guidance by LURE peptides.

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  135. .The AMOR Arabinogalactan Sugar Chain Induces Pollen-Tube Competency to Respond to Ovular Guidance. Reviewed

    Mizukami AG, Inatsugi R, Jiao J, Kotake T, Kuwata K, Ootani K, Okuda S, Sankaranarayanan S, Sato Y, Maruyama D, Iwai H, Garénaux E, Sato C, Kitajima K, Tsumuraya Y, Mori H, Yamaguchi J, Itami K, Sasaki N, Higashiyama T

    Curr Biol.   Vol. 26 ( 8 ) page: 1091-7   2016.4

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  136. 2,11-Dibromo-13,14-dimesityl-5,8-dioxapentaphene: A Stable and Twisted Polycyclic System Containing the o-Quinodimethane Skeleton.

    Sato C, Suzuki S, Kozaki M, Okada K

    Organic letters   Vol. 18 ( 5 ) page: 1052 - 5   2016.3

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    DOI: 10.1021/acs.orglett.6b00171

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  137. Spread of reduced activity of STX promoter throughout Great Journey

    Fujito Naoko, Satta Yoko, Hane Masaya, Matsui Atsushi, Kitajima Ken, Sato Chihiro, Hayakawa Toshiyuki

    GENES & GENETIC SYSTEMS   Vol. 90 ( 6 ) page: 379 - 379   2015.12

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  138. Flagellasialin: Highly Glycosylated Gpi-Anchored Protein Involved in Intracellular Ca2+ Regulation in Sea Urchin Sperm Reviewed

    Takeru Kanazawa, Eriko Suzuki, Shinji Miyata, Chihiro Sato, Ken Kitajima

    Glycoscience: Biology and Medicine     page: 883 - 890   2015.1

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    Very little knowledge has been available on structure and function of heavily glycosylated proteins, whose glycan parts amount to more than 90 % of the whole molecules, mainly because of technical difficulties in detection and determination of structure. In 2004 flagellasialin was discovered as a major cell surface component in the lipid rafts of sea urchin sperm. The extremely high content of glycan chains completely prevented detecting this glycoprotein by conventional protein staining methods, such as Coomassie Brilliant Blue and silver staining. To make this glycoprotein visible, monoclonal antibodies 4F7 and 3G9 were developed as specific probes for detecting glycan chains of flagellasialin. The frequent occurrence of GPI-anchored proteins in lipid rafts is well recognized. Flagellasialin was actually demonstrated to contain a GPI-anchor, based on three lines of experiments. In addition to the computational predictions from the cDNA sequence and the phosphatidylinositolspecific phospholipase C treatment of the sperm membrane fraction, nitrous acid deamination of a flagellasialin-blotted membrane was newly introduced. The immunostaining of flagellasialin on the membrane disappeared after the nitrous acid treatment of the blotted membrane. This method was easy and effective for identifying flagellasialin as a GPI-anchored protein.

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  139. Polysialic acid Reviewed

    Chihiro Sato, Ken Kitajima

    Glycoscience: Biology and Medicine     page: 519 - 528   2015.1

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    Polysialic acid (polySia), particularly α2,8-linked polyNeu5Ac (DP ¼ 8 ~ 400), is a unique polymer of sialic acid (Sia) and modifies neural cell adhesion molecule (NCAM) spatiotemporally in embryonic brains. PolySia is involved in cell migration, neural outgrowth, axonal guidance, synaptic plasticity, and the development of normal neural circuits and neurogenesis due to its anti- adhesive property and spatiotemporal expression. Recent improvements of polySia-detection methods have told us that polySia expression persists in certain areas of the adult brain, such as the olfactory bulb, hippocampus, subventricular zone, thalamus, prefrontal cortex, and amygdala, where neural plasticity, remodeling of neural connections, or neural generation is ongoing. Importantly, several lines of evidence have raised a new concept of polySia function that polySia works as an attractive field that associates with particular ion channels and neurologically active molecules, such as neurotrophic factor (BDNF), growth factor (FGF2), and neurotransmitter (dopamine), to regulate their involved signaling. It has been shown that polySia is involved in learning, memory, circadian rhythm, and social behaviors using polySia-impaired mice. In addition, SNPs and some deletions in genes encoding the polysialyltransferase and NCAM have been reported from patients of schizophrenia, mood disorder and autism. Such impairments of polySia structure derived from polysialyltransferase gene alterations might influence the polySia function not only as the repulsive field but also as the attractive field.

    DOI: 10.1007/978-4-431-54841-6_117

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  140. Polysialyltransferase assay Reviewed

    Masaya Hane, Ken Kitajima, Chihiro Sato

    Glycoscience: Biology and Medicine     page: 529 - 534   2015.1

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    It has been shown that polysialic acid (polySia) is an indispensable component for viability of mouse during development. Especially, it is deeply involved in normal brain function in mouse and human, affecting cell adhesion, cell migration, cell growth, neurogenesis, and synaptogenesis. PolySia is synthesized by two polysialyltransferases, ST8SIA2 and ST8SIA4. Mice deficient in both enzymes show lethality soon after birth. Mice deficient in either ST8SIA2 or ST8SIA4 show significant impairments in learning, memory, circadian rhythm, and social behavior. Interestingly, different phenotypes are often observed between ST8SIA2- and ST8SIA4-deficient mice. Therefore, characterization of these enzymes in vitro and in vivo and precise understanding of the structurefunction relationship of polySia become more and more important. In this chapter, assay procedures for in vitro activity of the polysialyltransferases are focused on to obtain clear results of their in vitro activities that are often said to be very weak compared with other sialyltransferases.

    DOI: 10.1007/978-4-431-54841-6_118

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  141. Discovery, primary, and crystal structures and capacitation-related properties of a prostate-derived heparin-binding protein WGA16 from boar sperm. Reviewed

    Garénaux E, Kanagawa M, Tsuchiyama T, Hori K, Kanazawa T, Goshima A, Chiba M, Yasue H, Ikeda A, Yamaguchi Y, Sato C, Kitajima K.

    J Biol Chem.   Vol. 290   page: 5484-501   2015

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  142. Protective effects of polysialic acid on proteolytic cleavage of FGF2 and proBDNF/BDNF. Reviewed

    Hane M, Matsuoka S, Ono S, Miyata S, Kitajima K, Sato C.

    Glycobiology   Vol. 25   page: 1112-24   2015

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  143. Sulfatide-Hsp70 interaction promotes Hsp70 clustering and stabilizes binding to unfolded protein. Reviewed

    Harada Y, Sato C, Kitajima K.

    Biomolecules.   Vol. 5   page: 958-73   2015

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  144. Ganglioside contained in the neuronal tissue-enriched acidic protein of 22 kDa (NAP-22) fraction prepared from the detergent-resistant membrane microdomain of rat brain inhibits the phosphatase activity of calcineurin. Reviewed

    Kobayashi Y, da Silva R, Kumanogoh H, Miyata S, Sato C, Kitajima K, Nakamura S, Morita M, Hayashi F, Maekawa S.

    J Neurosci Res.   Vol. 93   page: 1462-70   2015

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  145. Rapid Trimming of Cell Surface Polysialic Acid (PolySia) by Exovesicular Sialidase Triggers Release of Preexisting Surface Neurotrophin. Reviewed

    Sumida M, Hane M, Yabe U, Shimoda Y, Pearce OM, Kiso M, Miyagi T, Sawada M, Varki A, Kitajima K, Sato C.

    J Biol Chem.   Vol. 290   page: 13202-14   2015

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  146. Sulfatide-Hsp70 interaction promotes Hsp70 clustering and stabilizes binding to unfolded protein. Reviewed

    Harada Y, Sato C, Kitajima K

    Biomolecules.   Vol. 5   page: 958-73   2015

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  147. Advanced Technologies in Sialic Acid and Sialoglycoconjugate Analysis Reviewed

    Ken Kitajima, Nissi Varki, Chihiro Sato

    SIALOGLYCO CHEMISTRY AND BIOLOGY II: TOOLS AND TECHNIQUES TO IDENTIFY AND CAPTURE SIALOGLYCANS   Vol. 367   page: 75 - 103   2015

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    Although the structural diversity of sialic acid (Sia) is rapidly expanding, understanding of its biological significance has lagged behind. Advanced technologies to detect and probe diverse structures of Sia are absolutely necessary not only to understand further biological significance but also to pursue medicinal and industrial applications. Here we describe analytical methods for detection of Sia that have recently been developed or improved, with a special focus on 9-O-acetylated N-acetylneuraminic acid (Neu5,9Ac), N-glycolylneuraminic acid (Neu5Gc), deaminoneuraminic acid (Kdn), O-sulfated Sia (SiaS), and di-, oligo-, and polysialic acid (diSia/oligoSia/polySia) in glycoproteins and glycolipids. Much more attention has been paid to these Sia and sialoglycoconjugates during the last decade, in terms of regulation of the immune system, neural development and function, tumorigenesis, and aging.

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  148. Ganglioside contained in the neuronal tissue-enriched acidic protein of 22 kDa (NAP-22) fraction prepared from the detergent-resistant membrane microdomain of rat brain inhibits the phosphatase activity of calcineurin. Reviewed

    Kobayashi Y, da Silva R, Kumanogoh H, Miyata S, Sato C, Kitajima K, Nakamura S, Morita M, Hayashi F, Maekawa S

    J Neurosci Res.   Vol. 93   page: 1462-70   2015

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  149. Discovery, primary, and crystal structures and capacitation-related properties of a prostate-derived heparin-binding protein WGA16 from boar sperm. Reviewed

    Garénaux E, Kanagawa M, Tsuchiyama T, Hori K, Kanazawa T, Goshima A, Chiba M, Yasue H, Ikeda A, Yamaguchi Y, Sato C, Kitajima K

    J Biol Chem.   Vol. 290   page: 5484-501   2015

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  150. Protective effects of polysialic acid on proteolytic cleavage of FGF2 and proBDNF/BDNF. Reviewed

    Hane M, Matsuoka S, Ono S, Miyata S, Kitajima K, Sato C

    Glycobiology   Vol. 25   page: 1112-24   2015

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  151. Rapid Trimming of Cell Surface Polysialic Acid (PolySia) by Exovesicular Sialidase Triggers Release of Preexisting Surface Neurotrophin. Reviewed

    Sumida M, Hane M, Yabe U, Shimoda Y, Pearce OM, Kiso M, Miyagi T, Sawada M, Varki A, Kitajima K, Sato C

    J Biol Chem.   Vol. 290   page: 13202-14   2015

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  152. Adaptive evolution of the promoter region of the Sialyltransferase 8B (STX) gene

    Fujito Naoko T., Hayakawa Toshiyuki, Hane Masaya, Kitajima Ken, Sato Chihiro, Satta Yoko

    GENES & GENETIC SYSTEMS   Vol. 89 ( 6 ) page: 327-327   2014.12

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  153. Tissue-Specific Posttranslational Modification Allows Functional Targeting of Thyrotropin Reviewed

    Keisuke Ikegami, Xiao-Hui Liao, Yuta Hoshino, Hiroko Ono, Wataru Ota, Yuka Ito, Taeko Nishiwaki-Ohkawa, Chihiro Sato, Ken Kitajima, Masayuki Iigo, Yasufumi Shigeyoshi, Masanobu Yamada, Yoshiharu Murata, Samuel Refetoff, Takashi Yoshimura

    CELL REPORTS   Vol. 9 ( 3 ) page: 801 - 10   2014.11

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    Thyroid-stimulating hormone (TSH; thyrotropin) is a glycoprotein secreted from the pituitary gland. Pars distalis-derived TSH (PD-TSH) stimulates the thyroid gland to produce thyroid hormones (THs), whereas pars tuberalis-derived TSH (PT-TSH) actsonthe hypothalamus to regulate seasonal physiology and behavior. However, it had not been clear how these two TSHs avoid functional crosstalk. Here, we show that this regulation ismediated by tissue-specific glycosylation. Although PT-TSH is released into the circulation, it does not stimulate the thyroid gland. PD-TSH is known to have sulfated biantennary N-glycans, and sulfated TSH is rapidly metabolized in the liver. In contrast, PT-TSH has sialylated multibranched N-glycans; in the circulation, it forms the macro-TSH complex with immunoglobulin or albumin, resulting in the loss of its bioactivity. Glycosylation is fundamental to a wide range of biological processes. This report demonstrates its involvement in preventing functional crosstalk of signaling molecules in the body.

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  154. An anti-schizophrenic drug affects the surface expression of polySia-NCAM in IMR-32 human neuroblastoma cells

    Nishimura Saki, Hane Masaya, Niimi Yuki, Kitajima Ken, Sato Chihiro

    GLYCOBIOLOGY   Vol. 24 ( 11 ) page: 1193-1193   2014.11

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  155. Critical importance of the surface expression of alpha 2,6-sialosides during early development of medaka fish

    Kitajima Ken, Wu Di, Tajima Katsue, Maruyama Emi, Yasukawa Yuko, Chang Lan-Yi, Taniguchi Yoshihito, Kamei Yasuhiro, Adachi Tomoko, Hashimoto Hisashi, Hibi Masahiko, Fujita Akiko, Sato Chihiro

    GLYCOBIOLOGY   Vol. 24 ( 11 ) page: 1110-1110   2014.11

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  156. Insight into single nucleotide polymorphisms (SNPs) of the polysialyltransferase ST8SIA2/STX in psychiatric disorders

    Hane Masaya, Nishimura Saki, Hayakawa Toshiyuki, Kitajima Ken, Sato Chihiro

    GLYCOBIOLOGY   Vol. 24 ( 11 ) page: 1206-1206   2014.11

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  157. The Synthesis of Sialo-containing Glycopolymers by pi-Allyl Nickel Catalyzed Coordination Polymerization

    Ohira Shuichi, Yasuda Yu, Sato Chihiro, KItajima Ken, Tomita Ikuyoshi, Takahashi Takashi, Tanaka Hiroshi

    GLYCOBIOLOGY   Vol. 24 ( 11 ) page: 1119-1120   2014.11

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  158. Interaction of 70-kDa heat shock protein with glycosaminoglycans and acidic glycopolymers Reviewed

    Yoichiro Harada, Estelle Garenaux, Takehiro Nagatsuka, Hirotaka Uzawa, Yoshihiro Nishida, Chihiro Sato, Ken Kitajima

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   Vol. 453 ( 2 ) page: 229 - 34   2014.10

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    Interaction of Hsp70 with natural and artificial acidic glycans is demonstrated based on the native PAGE analysis. Hsp70 interacts with acidic glycopolymers that contain clustered sulfated and di-sialylated glycan moieties on a polyacrylamide backbone, but not with neutral or mono-sialylated glycopolymers. Hsp70 also interacts and forms a large complex with heparin, heparan sulfate, and dermatan sulfate that commonly contain 2-O-sulfated iduronic acid residues, but not with other types of glycosaminoglycans (GAGs). Hsp70 consists of the N-terminal ATPase domain and the C-terminal peptide-binding domain. The interaction analyses using the recombinant N- and C-terminal half domains show that the ATPase domain mediates the direct interaction with acidic glycans, while the peptide-binding domain stabilizes the large complexes with particular GAGs. To our knowledge, this is the first demonstration of direct binding of Hsp70 to the particular GAGs. This property may be involved in the physiological functions of Hsp70 at the plasma membrane and extracellular environments. (C) 2014 Elsevier Inc. All rights reserved

    DOI: 10.1016/j.bbrc.2014.05.137

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  159. ST8 alpha-N-acetyl-neuraminide alpha- 2,8-sialyltransferase 2 (ST8SIA2)

    Sato C.

    Handbook of Glycosyltransferases and Related Genes, Second Edition   Vol. 2   page: 781-795   2014.1

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    DOI: 10.1007/978-4-431-54240-7_77

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  160. Polysialic acid: Biosynthesis, novel functions and applications. Reviewed

    Colley K., Kitajima K, Sato C.

    Critical Reviews in Biochemistry and Molecular Biology     page: 1-35   2014

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  161. Tissue-specific post-translational modification allows functional targeting of thyrotropin. Reviewed

    Ikegami K, Liao X-H, Hoshino Y, Ono H, Ota W, Ito Y, Nishiwaki-Ohkawa T, Sato C, Kitajima K, Iigo M, Shigeyoshi Y, Yamada M, Murata Y, Refetoff S, Yoshimura T.

    Cell Reports   Vol. 9   page: 1-9   2014

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  162. Comparison of analytical methods to detect polysialic acid. Reviewed

    Nishimura S, Hane M, Niimi Y, Miyata S, Kitajima K, and Sato C.

    Journal of Glycomics and Lipidomics   Vol. 4   page: 113   2014

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  163. Interaction of 70-kDa heat shock protein with glycosaminoglycans and acidc glycopolymers. Reviewed

    Harada Y, Garenaux E, Nagatsuka T, Uzawa H, Nishida Y, Sato C, and Kitajima K.

    Biochem Biophys Res Commun.   Vol. 453   page: 229-234   2014

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  164. Frontal affinity chromatography: Practice of weak interaction analysis between lectins and fluorescently labeled oligosaccharides Reviewed

    Chihiro Sato

    Methods in Molecular Biology   Vol. 1200   page: 257 - 64   2014

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    Frontal affinity chromatography (FAC) is a simple and effective method that is applicable to the analysis of interactions between glycans and glycan-recognition proteins, including lectins, with weak affinity ranging from 10&lt
    sup&gt
    -4&lt
    /sup&gt
    to 10&lt
    sup&gt
    -6&lt
    /sup&gt
    (M) in terms of dissociation constant (K &lt
    inf&gt
    d&lt
    /inf&gt
    ). Using conventional instruments, such as a high-performance liquid chromatography (HPLC) system equipped with pump, injector, (fluorescent) detector, and data recorder, the dissociation constants for weak glycan-based interactions can be easily determined with high throughput and accuracy. Notably, if the glycans are labeled with fluorescent dyes, only a small amount of glycans is required for the analysis. Fluorescent labeling of glycans is a common technique, and an increasing number of fluorescent-labeled glycans are commercially available. In this chapter, an advanced FAC method using fluorescent-labeled glycans is described. © 2014 Springer Science+Business Media New York.

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  165. Comparison of analytical methods to detect polysialic acid. Reviewed

    Nishimura S, Hane M, Niimi Y, Miyata S, Kitajima K, Sato C

    Journal of Glycomics and Lipidomics   Vol. 4   page: 113   2014

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  166. Polysialic acid: Biosynthesis, novel functions and applications. Reviewed

    Colley K, Kitajima K, Sato C

    Critical Reviews in Biochemistry and Molecular Biology     page: 1-35   2014

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  167. NMR study into the mechanism of recognition of the degree of polymerization by oligo/polysialic acid antibodies. Reviewed

    Hanashima S, Sato C, Tanaka H, Takahashi T, Kitajima K, Yamaguchi Y.

    Bioorg. Med. Chem.   Vol. 21   page: 6069-76   2013.10

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  168. NMR study into the mechanism of recognition of the degree of polymerization by oligo/polysialic acid antibodies Reviewed

    Shinya Hanashima, Chihiro Sato, Hiroshi Tanaka, Takashi Takahashi, Ken Kitajima, Yoshiki Yamaguchi

    BIOORGANIC & MEDICINAL CHEMISTRY   Vol. 21 ( 19 ) page: 6069 - 76   2013.10

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    Oligo/polysialic acids consisting of consecutive alpha(2,8)-linkages on gangliosides and glycoproteins play a role in cell adhesion and differentiation events in a manner that is dependent on the degree of polymerization (DP). Anti-oligo/polysialic acid antibodies often have DP-dependent antigenic specificity, and such unique antibodies are often used in biological studies for the detection and differentiation of oligo/polysialic acids. However, molecular mechanisms remain unclear. We here use NMR techniques to analyze the binding epitopes of the anti-oligo/polysialic acid monoclonal antibodies (mAb) A2B5 and 12E3. The mAb A2B5, which has a preference for trisialic acid, recognizes sialic acid residues at the non-reducing terminus and those in nascent units. On the other hand, mAb 12E3, which prefers oligo/polysialic acids of more than six sugar units, recognizes inner sialic acid residues. In both structural complexes, the interresidue transferred NOE correlations are significantly different from those arising from analogs of the free states, indicating that the bound and free sugar conformations are distinct. The ability of the two mAbs to distinguish the chain lengths comes from different binding epitopes and possibly from the conformational differences in the oligo/polysialic acids. Information on the recognition modes is needed for the structural design of immunoreactive antigens for the development of high-affinity anti-polysialic acid antibodies and of related vaccines against pathogenic, polysialic acid-coated bacteria. (c) 2013 Elsevier Ltd. All rights reserved.

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  169. Polysialic acid

    Sato C.

    Sialobiology: Structure, Biosynthesis and Function. Sialic Acid Glycoconjugates in Health and Disease     page: 33-75   2013.8

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    DOI: 10.2174/9781608053865113010005

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  170. Disialic, oligosialic and polysialic acids: distribution, functions and related disease Reviewed

    Chihiro Sato, Ken Kitajima

    JOURNAL OF BIOCHEMISTRY   Vol. 154 ( 2 ) page: 115 - 36   2013.8

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    Sialic acids (Sia) are involved in many biological activities and frequently exist as monosialyl residues at the non-reducing terminal end of glycoconjugates. Occasionally, polymerized structures in the form of disialic acid (diSia), oligosialic acid (oligoSia) and polysialic acid (polySia) are also found in glycoconjugates. In particular, polySia, which is an evolutionarily conserved epitope from sea urchin to humans, is one of the most biologically important glycotopes in vertebrates. The biological functions of polySia, especially on neural cell adhesion molecules, have been well studied and an in-depth body of knowledge concerning polySia has been accumulated. However, considerably less attention has been paid to glycoproteins containing di- and oligoSia groups. However, advances in analytical methods for detecting oligo/polymerized structures have allowed the identification and characterization of an increasing number of glycoproteins containing di/oligo/polySia chains in nature. In addition, sophisticated genetic techniques have also helped to elucidate the underlying mechanisms of polySia-mediated activities. In this review, recent advances in the study of the chemical properties, distribution and functions of di-, oligo- and polySia residues on glycoproteins are described.

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  171. ポリシアル酸による神経機能の調節. Invited Reviewed

    佐藤ちひろ、北島健

    実験医学   Vol. 31   page: 26--33   2013

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  172. Disialic, oligosialic, and polysialic acids: Distribution, biological functions, and related disease. Invited Reviewed

    Sato C., Kitajima K.

    J Biochem (Tokyo).   Vol. 154   page: 115-136   2013

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  173. Impact of structural aberrancy of polysialic acid and its synthetic enzyme ST8SIA2 in schizophrenia. Reviewed

    Sato C, Kitajima K.

    Front. Cell. Neurosci.   Vol. 7 ( 61 ) page: 1-11   2013

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  174. Crystal structure of anti-polysialic acid antibody single chain Fv fragment complexed with octasialic acid: insight into the binding preference for polysialic acid. Reviewed

    Nagae M, Ikeda A, Hane M, Hanashima S, Kitajima K, Sato C, Yamaguchi Y.

    J Biol Chem.   Vol. 288   page: 33784-96   2013

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  175. Occurrence of free deaminoneuraminic acid (KDN)-containing complex-type N-glycans in human prostate cancers. Reviewed

    Yabu M, Korekane H, Takahashi H, Ohigashi H, Ishikawa O, Hatano K, Nonomura N, Sato C, Kitajima K, Miyamoto Y.

    Glycobiology   Vol. 23   page: 634-642   2013

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  176. Polysialic Acid

    Sato Chihiro

    SIALOBIOLOGY: BIOSYNTHESIS, STRUCTURE AND FUNCTION: SIALIC ACID GLYCOCONJUGATES IN HEALTH AND DISEASE     page: 33-75   2013

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  177. ポリシアル酸による神経機能の調節. Invited Reviewed

    佐藤ちひろ, 北島健

    実験医学   Vol. 31   page: 26--33   2013

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  178. Crystal structure of anti-polysialic acid antibody single chain Fv fragment complexed with octasialic acid: insight into the binding preference for polysialic acid. Reviewed

    Nagae M, Ikeda A, Hane M, Hanashima S, Kitajima K, Sato C, Yamaguchi Y

    J Biol Chem.   Vol. 288   page: 33784-96   2013

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  179. Impact of structural aberrancy of polysialic acid and its synthetic enzyme ST8SIA2 in schizophrenia. Reviewed

    Sato C, Kitajima K

    Front. Cell. Neurosci.   Vol. 7 ( 61 ) page: 1-11   2013

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  180. Occurrence of free deaminoneuraminic acid (KDN)-containing complex-type N-glycans in human prostate cancers. Reviewed

    Yabu M, Korekane H, Takahashi H, Ohigashi H, Ishikawa O, Hatano K, Nonomura N, Sato C, Kitajima K, Miyamoto Y

    Glycobiology   Vol. 23   page: 634-642   2013

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  181. Pig sperm membrane microdomains contain a highly glycosylated 15-25-kDa wheat germ agglutinin-binding protein Reviewed

    Waraporn Kasekarn, Takeru Kanazawa, Kazuki Hori, Tomoyuki Tsuchiyama, Xue Lian, Estelle Garenaux, Kessiri Kongmanas, Nongnuj Tanphaichitr, Hiroshi Yasue, Chihiro Sato, Ken Kitajima

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   Vol. 426 ( 3 ) page: 356 - 62   2012.9

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    A highly glycosylated protein, which has unique, novel features in localization, structure, and potential function, is found in pig sperm, and named WGA-gp due to its high binding property with wheat germ agglutinin (WGA). WGA-gp is localized mainly in flagella and enriched in membrane microdomains or lipid rafts. It is not detected by ordinary protein staining methods due to a high content of both Nand O-glycans consisting of neutral monosaccharides. Interestingly. WGA-gp may be involved in intracellular Ca2+ regulation. Treatment of sperm with anti-WGA-gp antibody enhances the amplitude of Ca2+ oscillation without changing the basal intracellular Ca2+ concentrations. All these features of WGA-gp, except for different carbohydrate structures occupying most part of the molecules, are similar to those of flagellasialin in sea urchin sperm, which regulates the intracellular Ca2+ concentration. Presence of carbohydrate-enriched flagellar proteins involved in intracellular Ca2+ regulation may be a common feature among animal sperm. (c) 2012 Elsevier Inc. All rights reserved.

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  182. Metabolism of Vertebrate Amino Sugars with N-Glycolyl Groups RESISTANCE OF alpha 2-8-LINKED N-GLYCOLYLNEURAMINIC ACID TO ENZYMATIC CLEAVAGE Reviewed

    Leela R. L. Davies, Oliver M. T. Pearce, Matthew B. Tessier, Siavash Assar, Victoria Smutova, Maria Pajunen, Mizuki Sumida, Chihiro Sato, Ken Kitajima, Jukka Finne, Pascal Gagneux, Alexey Pshezhetsky, Robert Woods, Ajit Varki

    JOURNAL OF BIOLOGICAL CHEMISTRY   Vol. 287 ( 34 ) page: 28917 - 31   2012.8

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    The sialic acid (Sia) N-acetylneuraminic acid (Neu5Ac) and its hydroxylated derivative N-glycolylneuraminic acid (Neu5Gc) differ by one oxygen atom. CMP-Neu5Gc is synthesized from CMP-Neu5Ac, with Neu5Gc representing a highly variable fraction of total Sias in various tissues and among different species. The exception may be the brain, where Neu5Ac is abundant and Neu5Gc is reported to be rare. Here, we confirm this unusual pattern and its evolutionary conservation in additional samples from various species, concluding that brain Neu5Gc expression has been maintained at extremely low levels over hundreds of millions of years of vertebrate evolution. Most explanations for this pattern do not require maintaining neural Neu5Gc at such low levels. We hypothesized that resistance of alpha 2-8-linked Neu5Gc to vertebrate sialidases is the detrimental effect requiring the relative absence of Neu5Gc from brain. This linkage is prominent in polysialic acid (polySia), a molecule with critical roles in vertebrate neural development. We show that Neu5Gc is incorporated into neural polySia and does not cause in vitro toxicity. Synthetic polymers of Neu5Ac and Neu5Gc showed that mammalian and bacterial sialidases are much less able to hydrolyze alpha 2-8-linked Neu5Gc at the nonreducing terminus. Notably, this difference was not seen with acid-catalyzed hydrolysis of polySias. Molecular dynamics modeling indicates that differences in the three-dimensional conformation of terminal saccharides may partly explain reduced enzymatic activity. In keeping with this, polymers of N-propionylneuraminic acid are sensitive to sialidases. Resistance of Neu5Gc-containing polySia to sialidases provides a potential explanation for the rarity of Neu5Gc in the vertebrate brain.

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  183. Structural and functional impairments of polySia-NCAM synthesized by a mutated polysialyltransferase of a schizophrenic patient. Reviewed

    Hane M, Sumida M, Kitajima K, Sato C.

    Pure Appl Chem   Vol. 84   page: 1895-1906   2012.6

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  184. Structural and functional impairments of polySia-NCAM synthesized by a mutated polysialyltransferase of a schizophrenic patient. Reviewed

    Hane M, Sumida M, Kitajima K, Sato C

    Pure Appl Chem   Vol. 84   page: 1895-1906   2012.6

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  185. Sialidase NEU4 Hydrolyzes Polysialic Acids of Neural Cell Adhesion Molecules and Negatively Regulates Neurite Formation by Hippocampal Neurons Reviewed

    Kohta Takahashi, Junya Mitoma, Masahiro Hosono, Kazuhiro Shiozaki, Chihiro Sato, Kazunori Yamaguchi, Ken Kitajima, Hideyoshi Higashi, Kazuo Nitta, Hiroshi Shima, Taeko Miyagi

    JOURNAL OF BIOLOGICAL CHEMISTRY   Vol. 287 ( 18 ) page: 14816 - 26   2012.4

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    Modulation of levels of polysialic acid (polySia), a sialic acid polymer, predominantly associated with the neural cell adhesion molecule (NCAM), influences neural functions, including synaptic plasticity, neurite growth, and cell migration. Biosynthesis of polySia depends on two polysialyltransferases ST8SiaII and ST8SiaIV in vertebrate. However, the enzyme involved in degradation of polySia in its physiological turnover remains uncertain. In the present study, we identified and characterized a murine sialidase NEU4 that catalytically degrades polySia. Murine NEU4, dominantly expressed in the brain, was found to efficiently hydrolyze oligoSia and polySia chains as substrates in sialidase in vitro assays, and also NCAM-Fc chimera as well as endogenous NCAM in tissue homogenates of postnatal mouse brain as assessed by immunoblotting with anti-polySia antibodies. Degradation of polySia by NEU4 was also evident in neuroblastoma Neuro2a cells that were co-transfected with Neu4 and ST8SiaIV genes. Furthermore, in mouse embryonic hippocampal primary neurons, the endogenously expressed NEU4 was found to decrease during the neuronal differentiation. Interestingly, GFP- or FLAG-tagged NEU4 was partially co-localized with polySia in neurites and significantly suppressed their outgrowth, whereas silencing of NEU4 showed the acceleration together with an increase in polySia expression. These results suggest that NEU4 is involved in regulation of neuronal function by polySia degradation in mammals.

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  186. Sialidase NEU4 hydrolyzes polysialic acids of neural cell adhesion molecules and negatively regulates neurite formation by hippocampal neurons. Reviewed

    Takahashi K, Mitoma J, Hosono M, Shiozaki K, Sato C, Yamaguchi K, Kitajima K, Higashi H, Nitta K, Shima H, Miyagi T.

    J Biol Chem.   Vol. 287   page: 14816-14826   2012.3

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  187. Novel Regulation of Fibroblast Growth Factor 2 (FGF2)-mediated Cell Growth by Polysialic Acid Reviewed

    Sayaka Ono, Masaya Hane, Ken Kitajima, Chihiro Sato

    JOURNAL OF BIOLOGICAL CHEMISTRY   Vol. 287 ( 6 ) page: 3710 - 22   2012.2

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    Polysialic acid (polySia) is a unique polysaccharide that modifies neural cell adhesion molecule (NCAM) spatiotemporally. Recently, we demonstrated that polySia functions as a reservoir for several neurotrophic factors and neurotransmitters. Here, we showed the direct interaction between polySia and fibroblast growth factor-2 (FGF2) by native-PAGE, gel filtration, and surface plasmon resonance. The minimum chain length of polySia required for the interaction with FGF2 was 17. Compared with heparan sulfate, a well known glycosaminoglycan capable of forming a complex with FGF2, polySia formed a larger complex with distinct properties in facilitating oligomerization of FGF2, as well as in binding to FGF receptors. In polySia-NCAM-expressing NIH-3T3 cells, which were established by transfecting cells with either of the plasmids for the expression of the polysialyltransferases ST8SiaII/STX and ST8SiaIV/PST that can polysialylate NCAM, FGF2-stimulated cell growth, but not cell survival, was inhibited. Taken together, these results suggest that polySia-NCAM might be involved in the regulation of FGF2-FGF receptor signaling through the direct binding of FGF2 in a manner distinct from heparan sulfate.

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  188. Novel regulation of FGF2-mediated cell growth by polysialic acid. Reviewed

    Ono S, Hane M, Kitajima K, Sato C.

    J Biol Chem.     page: 3710-22.   2012

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  189. Sialome analysis of the cephalochordate Branchiostoma belcheri, a key organism for vertebrate evolution. Reviewed

    Guérardel Y, Chang LY, Fujita A, Coddeville B, Maes E, Sato C, Harduin-Lepers A, Kubokawa K, Kitajima K.

    Glycobiolgy     page: in press   2012

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  190. Sialome analysis of the cephalochordate Branchiostoma belcheri, a key organism for vertebrate evolution. Reviewed

    Guérardel Y, Chang LY, Fujita A, Coddeville B, Maes E, Sato C, Harduin-Lepers A, Kubokawa K, Kitajima K

    Glycobiolgy     page: in press   2012

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  191. Co-expression of two distinct polysialic acids, α2,8- and α2,9-linked polymers of N-acetylneuraminic acid, in distinct glycoproteins and glycolipids in sea urchin sperm. Reviewed

      Vol. 21   page: 1596-1605   2011.12

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  192. New function of polysialic acid and its involvement in neural activities

    Sato C.

    Seikagaku   Vol. 83 ( 3 ) page: 189-196   2011.12

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  193. Co-expression of two distinct polysialic acids, α2,8- and α2,9-linked polymers of N-acetylneuraminic acid, in distinct glycoproteins and glycolipids in sea urchin sperm. Reviewed

      Vol. 21   page: 1596-1605   2011.12

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  194. The Roles of Carbohydrate Binding in Cell Adhesion and Inflammation Reviewed

    Ken Kitajima, Chihiro Sato

    Carbohydrate Recognition: Biological Problems, Methods, and Applications     page: 33 - 63   2011.7

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    DOI: 10.1002/9781118017586.ch2

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  195. New function of polysialic acid and its involvement in neural activities

    SATO Chihiro, KITAJIMA Ken

      Vol. 83 ( 3 ) page: 189-196   2011.3

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  196. ポリシアル酸の新機能と神経形成維持機構 Reviewed

    佐藤ちひろ, 北島 健

    生化学   Vol. 83   page: 189-196   2011.3

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  197. [New function of polysialic acid and its involvement in neural activities].

    Sato C, Kitajima K

    Seikagaku. The Journal of Japanese Biochemical Society   Vol. 83 ( 3 ) page: 189-96   2011.3

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  198. New functions of polysialic acid and its relationship to schizophrenia Reviewed

    Sato C and Kitajima K

      Vol. 23   page: 221-238   2011

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  199. Structural and functional impairments of polysialic acid by a mutated polysialyltransferase found in schizophrenia Reviewed

    Isomura, R., Kitajima, K., and Sato, C.

    J. Biol. Chem.   Vol. 286   page: 21535-21545   2011

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  200. Human KDN (deaminated neuraminic acid) and its elevated expression in cancer cells: Mechanism and significance Reviewed

    Sadako Inoue, Ken Kitajima, Chihiro Sato, Shinji Go

    Advances in Experimental Medicine and Biology   Vol. 705   page: 669 - 78   2011

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    Sialic acids are a family of nine-carbon carboxylated sugars having a nonulosonate skeletal structure (Fig. 35.1). This structure is uniquely different from that of other sugar units of animal glycans. The most popular sialic acid is N-acetylneuraminic acid (Neu5Ac), which is universally found on cell surface glycocalyx and in secreted glycoproteins of vertebrates and some invertebrates. Sialic acids have low acid-base dissociation constants and give a negative charge on the cell surface under a wide range of physiological pH. In nature, more than 50 kinds of sialic acids are known. Nearly all of them are derived from Neu5Ac by a substitution on the hydroxyl groups (e.g., O-acetyl-Neu5Ac) and/or a hydroxylation of the N-acetyl group (e.g., N-glycolylneuraminic acid, Neu5Gc). Each modified sialic acid has properties different from those of Neu5Ac and is believed to contribute to specific physiological functions. In animal cells, sialic acids are most frequently the terminal sugars of cell surface glycolipids and glycoproteins, and any change that occurs on sialic acids can considerably influence the biological properties of a cell. © 2011 Springer Science+Business Media, LLC.

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  201. Mechanism Regulating Ca2+-dependent Mechanosensory Behaviour in Sea Urchin Spermatozoa

    Kambara Yuka, Shiba Kogiku, Yoshida Manabu, Sato Chihiro, Kitajima Ken, Shingyoji Chikako

    CELL STRUCTURE AND FUNCTION   Vol. 36 ( 1 ) page: 69-82   2011

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  202. Mechanism Regulating Ca2+-dependent Mechanosensory Behaviour in Sea Urchin Spermatozoa Reviewed

    Yuka Kambara, Kogiku Shiba, Manabu Yoshida, Chihiro Sato, Ken Kitajima, Chikako Shingyoji

    CELL STRUCTURE AND FUNCTION   Vol. 36 ( 1 ) page: 69 - 82   2011

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    Flagellar movement of the sea urchin sperm is regulated by intracellular Ca2+. Flagellasialin, a polysialic acid-containing glycoprotein, as well as other membrane proteins seems responsible for the Ca2+ control. To elucidate the mechanism of Ca2+ dynamics underlying flagellar movement, we analysed the sperm's mechanosensory behavioural responses by using microtechniques. In sea water containing 10 mM Ca2+, the sperm swim in circular paths. When a mechanical stimulus was applied to the sperm head with a glass microstylus, the sperm showed a series of flagellar responses, consisting of a stoppage of beating (quiescence) and a recovery of swimming in a straight path, followed by swimming in a circular path again; as the result the sperm avoided the obstacle. Ca2+-imaging with Fluo-4 showed that the intracellular Ca2+ was high in the quiescence and gradually decreased after that. The effects of blockers and antibodies against candidate components revealed that the Ca2+ influx was induced by Ca2+ channels and the Ca2+ efflux was induced by a flagellasialin-related Ca2+-efflux system, plasma membrane Ca2+-ATPases and the K+-dependent Na+/Ca2+ exchanger. The results show that the Ca2+-dependent mechanosensory behaviour of the sea urchin sperm is regulated by organized functioning of the membrane environment including the plasma membrane proteins and flagellasialin.

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  203. Structural and functional impairments of polysialic acid by a mutated polysialyltransferase found in schizophrenia Reviewed

    Isomura, R, Kitajima, K, Sato, C

    J. Biol. Chem.   Vol. 286   page: 21535-21545   2011

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  204. New functions of polysialic acid and its relationship to schizophrenia Reviewed

    Sato C, Kitajima K

      Vol. 23   page: 221-238   2011

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  205. Developmental stage-dependent expression of an alpha 2,8-trisialic acid unit on glycoproteins in mouse brain Reviewed

    Emi Inoko, Yuji Nishiura, Hiroshi Tanaka, Takashi Takahashi, Koichi Furukawa, Ken Kitajima, Chihiro Sato

    GLYCOBIOLOGY   Vol. 20 ( 7 ) page: 916 - 28   2010.7

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    The monoclonal antibody mAb.A2B5 is a marker for the detection of oligodendrocyte progenitor cells that differentiate into type-2 astrocytes and oligodendrocytes. It is also a useful antibody for separating these cells from other lineage populations. The epitope of this antibody is considered to be the gangliosides GT3 and GQ1c. In this study, we sought to define more precisely the structure of the epitope. Accordingly, we chemically synthesized defined oligosialic acid structures linked to phosphatidylethanolamine and bovine serum albumin and used these to determine the antigenic specificity. mAb.A2B5 recognized the Neu5Ac alpha 2 -&gt; 8Neu5Ac alpha 2 -&gt; 8Neu5Ac alpha -&gt; structure on both glycolipids and glycoproteins. We then examined whether the mAb.A2B5 epitope exists on glycoproteins in developing mouse brains. Western blot analyses revealed the expression of four glycoproteins reactive with the mAb.A2B5, and their expression was dependent on the stage of neural development. All the immunoreactivity in these glycoproteins with mAb.A2B5 disappeared after sialidase treatment and were resistant to chloroform/methanol extraction. These epitopes were also detected in brain homogenates from both GD3 synthetase-null and GD3/GD2 synthetase double null mice. These findings show that the alpha 2,8-trisialic acid (triSia) unit recognized by mAb.A2B5 resides not only on gangliosides but also on glycoproteins in developing mouse brain. We postulate that the triSia structure on glycoproteins may be involved in oligodendrocyte differentiation, similar to the case with the alpha 2,8-triSia structure on gangliosides. Real time polymerase chain reaction analysis of the developmental expression of all known ST8Sia genes, which are responsible for the biosynthesis of alpha 2,8-linked Sia residues, showed that ST8Sia III gene expression correlated with expression of the triSia epitope. We suggest that ST8Sia III is the principal sialyltransferase responsible for synthesis of the alpha 2,8-triSia units on glycoproteins.

    DOI: 10.1093/glycob/cwq049

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  206. 糖鎖と統合失調症 Invited

    佐藤ちひろ

    生体の科学   Vol. 61   page: 160-166   2010

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  207. IgG2 dominancy and carbohydrate recognition specificity of C3H/He mouse antibodies directed to cross-reactive carbohydrate determinants (CCDs) bearing beta-(1,2)-xylose and alpha-(1,3)-fucose. Reviewed

    Hino S, Umeda F, Inumaru S, Aoki N, Sato, C., Okajima T, Nadano D, Matsuda T.

    Immunol Lett   Vol. 133   page: 28-34   2010

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  208. Survival signals of hepatic stellate cells in liver regeneration are regulated by glycosylation changes in rat vitronectin, especially decreased sialylation. Reviewed

    Sano K, Miyamoto Y, Kawasaki N, Hashii N, Itoh S, Murase M, Date K, Yokoyama M, Sato, C., Kitajima K, Ogawa H.

    J Biol Chem   Vol. 285   page: 17301-17309   2010

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  209. Extensive enrichment of N-glycolylneuraminic acid in extracellular sialoglycoproteins abundantly synthesized and secreted by human cancer cells. Reviewed

    Inoue, S., Sato, C. and Kitajima, K

    Glycobiology   Vol. 20   page: 752-762   2010

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  210. Developmental stage-dependent expression of an α2,8-trisialic acid unit on glycoproteins in mouse brain. Reviewed

    Inoko, E., Nishiura, Y., Tanaka, H., Takahashi, T., Furukawa, K., Kitajima, K., and Sato, C.

    Glycobiology   Vol. 20   page: 916-928   2010

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  211. Survival signals of hepatic stellate cells in liver regeneration are regulated by glycosylation changes in rat vitronectin, especially decreased sialylation. Reviewed

    Sano K, Miyamoto Y, Kawasaki N, Hashii N, Itoh S, Murase M, Date K, Yokoyama M, Sato, C, Kitajima K, Ogawa H

    J Biol Chem   Vol. 285   page: 17301-17309   2010

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  212. 糖鎖と統合失調症 Invited

    佐藤ちひろ

    生体の科学   Vol. 61   page: 160-166   2010

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  213. Extensive enrichment of N-glycolylneuraminic acid in extracellular sialoglycoproteins abundantly synthesized and secreted by human cancer cells. Reviewed

    Inoue, S, Sato, C, Kitajima, K

    Glycobiology   Vol. 20   page: 752-762   2010

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  214. IgG2 dominancy and carbohydrate recognition specificity of C3H/He mouse antibodies directed to cross-reactive carbohydrate determinants (CCDs) bearing beta-(1,2)-xylose and alpha-(1,3)-fucose. Reviewed

    Hino S, Umeda F, Inumaru S, Aoki N, Sato, C, Okajima T, Nadano D, Matsuda T

    Immunol Lett   Vol. 133   page: 28-34   2010

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  215. Periodate-resistant carbohydrate epitopes recognized by IgG and IgE antibodies from some of the immunized mice and patients with allergy Reviewed

    Shingo Hino, Takeshi Matsubara, Atsuo Urisu, Naohito Aoki, Chihiro Sato, Tetsuya Okajima, Daita Nadano, Tsukasa Matsuda

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   Vol. 380 ( 3 ) page: 632 - 7   2009.3

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    The beta(1,2)-xylose- and/or alpha(1,3)-fucose-containing cross-reactive carbohydrate determinants (CCDs) are present in various plant and insect N-glycans, and have been attracted as potential antigens in IgE-mediated allergies and immunologically undesired post-translational products on some recombinant therapeutic proteins. By using ELISA and immunoblotting, CCDs-specific IgG and IgE antibodies from some, but not all, of mice and humans were found to fully retain their binding activity after a typical periodate-treatment to CCDs, which did cause the CCDs&apos; antigenic activity to those from the other mice and rabbits to disappear almost completely. Furthermore, the mouse IgE recognizing the periodate-resistant CCDs induced the CCDs/IgE-dependent degranulation of rat basophilic RBL-2H3 cells. These findings indicate that in some cases CCDs include those dependent of the core trisaccharide more strongly than the terminal xylose and fucose, which might have been screened out in the CCDs analyses based on the loss of antibody-binding by the periodate-treatment. (C) 2009 Elsevier Inc. All rights reserved.

    DOI: 10.1016/j.bbrc.2009.01.138

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  216. Membrane microdomains from early gastrula embryos of medaka, Oryzias latipes are a plat form of E-cadherin and carbohydrate-mediated cell-cell interactions during epiboly Reviewed

    Adachi T., Sato, C., Kishi, Y., Totani, K., Murata, T., Usui, T., and Kitajima, K

    Glycoconjugate J.   Vol. 26   page: 285-299   2009

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  217. Complex formation of a brain-derived neurotrophic factor and glycosaminoglycans. Reviewed

    Kanato, Y., Kitajima, K. and Sato, C.

    Biosci. Biotech. Biosci.   Vol. 12   page: 1044-1053   2009

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  218. Developmental regulation of oligosialylation in zebrafish. Reviewed

    Chang LY, Harduin-Lepers A, Kitajima K, Sato C, Huang CJ, Khoo KH, Guérardel Y.

    Glycoconjugate J.   Vol. 26   page: 247-61   2009

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  219. Complex formation of a brain-derived neurotrophic factor and glycosaminoglycans. Reviewed

    Kanato, Y, Kitajima, K, Sato, C

    Biosci. Biotech. Biosci.   Vol. 12   page: 1044-1053   2009

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  220. Developmental regulation of oligosialylation in zebrafish. Reviewed

    Chang LY, Harduin-Lepers A, Kitajima K, Sato C, Huang CJ, Khoo KH, Guérardel Y

    Glycoconjugate J.   Vol. 26   page: 247-61   2009

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  221. Membrane microdomains from early gastrula embryos of medaka, Oryzias latipes are a plat form of E-cadherin and carbohydrate-mediated cell-cell interactions during epiboly Reviewed

    Adachi T, Sato, C, Kishi, Y, Totani, K, Murata, T, Usui, T, Kitajima, K

    Glycoconjugate J.   Vol. 26   page: 285-299   2009

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  222. [Regulation of signal transduction by polysialic acid]. Reviewed

    Sato C, Kitajima K

    Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme   Vol. 53 ( 12 Suppl ) page: 1577 - 1583   2008.9

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  223. Direct binding of polysialic acid to a brain-derived neurotrophic factor depends on the degree of polymerization Reviewed

    Kanato, Y, Kitajima, K., and Sato, C.

    Glycobiology   Vol. 12   page: 1044-1053   2008

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  224. ポリシアル酸構造による細胞シグナルの調節機構 Invited

    佐藤ちひろ、北島健

    蛋白質核酸酵素   Vol. 53 ( 1577-1583 )   2008

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  225. Direct binding of polysialic acid to a brain-derived neurotrophic factor depends on the degree of polymerization Reviewed

    Kanato, Y, Kitajima, K, Sato, C

    Glycobiology   Vol. 12   page: 1044-1053   2008

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  226. Glycobiology of polysialic acids on sea urchin gametes

    Miyata Shinji, Sato Chihiro, Kitajima Ken

    TRENDS IN GLYCOSCIENCE AND GLYCOTECHNOLOGY   Vol. 19 ( 106 ) page: 85-98   2007.3

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  227. Identification of an inflammation-inducible serum protein recognized by anti-disialic acid antibodies as carbonic anhydrase II Reviewed

    Zenta Yasukawa, Chihiro Sato, Ken Kitajima

    JOURNAL OF BIOCHEMISTRY   Vol. 141 ( 3 ) page: 429 - 41   2007.3

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    Acute-phase proteins are an important marker of inflammation and sometimes have a role in the general defense response towards tissue injury. In the present study, we identified a 32-kDa protein that was immunoreactive with monoclonal antibody 2-4B (mAb.2-4B), which is specific to di/oligoNeu5Gc structures, and that behaved as an acute-phase protein following stimulation with either turpentine oil or lipopolysaccharides. The 32-kDa protein was identified as carbonic anhydrase II (CA-II), based on matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analyses of the purified protein. Mouse and human CA-II was immunoreactive and immunoprecipitated with mAb.2-4B, but contained no sialic acid. In addition to mAb.2-4B, the mAb. S2-566 an antibody specific for diNeu5Ac-containing glycans, recognized the CA-II, whereas an anti-oligo/polysialic acid antibody did not. These results indicate that a part of the CA-II protein structure mimics the disialic acid structure recognized by the monoclonal antibodies. This is the first report that CA-II circulates in the serum following inflammation.

    DOI: 10.1093/jb/mvm047

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  228. Heterocomplex formation and cell-surface accumulation of hen's serum zona pellucida B1 (ZPB1) with ZPC expressed by a mammalian cell line (COS-7): A possible initiating step of egg-envelope matrix construction

    Okumura Hiroki, Aoki Naohito, Sato Chihiro, Nadano Daita, Matsuda Tsukasa

    BIOLOGY OF REPRODUCTION   Vol. 76 ( 1 ) page: 9-18   2007.1

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  229. Heterocomplex formation and cell-surface accumulation of hen's serum zona pellucida B1 (ZPB1) with ZPC expressed by a mammalian cell line (COS-7): A possible initiating step of egg-envelope matrix construction Reviewed

    Hiroki Okumura, Naohito Aoki, Chihiro Sato, Daita Nadano, Tsukasa Matsuda

    BIOLOGY OF REPRODUCTION   Vol. 76 ( 1 ) page: 9 - 18   2007.1

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    The egg envelope, referred to as zona pellucida (ZP) in mammalian eggs, is a fibrous and noncollagenous extracellular matrix surrounding vertebrate eggs, and composed of three to four homologous glycoproteins with a common ZP domain. In birds, a liver-derived ZP glycoprotein (ZP1/ZPB1) is transported through the bloodstream to ovarian follicles and joins the egg-envelope matrix construction together with the other ZP glycoproteins, such as ZPC and ZPD/ZPX2, both secreted from follicular granulosa cells. We report here that, through its ZP domain, ZPB1 specifically associates with ZPC, which might lead to the construction of egg-envelope matrix. The ZPB1 in laying hen's serum specifically bound to ZPC, but not to ZPX2, separated by SDS-PAGE and blotted on a membrane. Hemagglutinin (HA)-tagged ZPC expressed in a mammalian cell line (COS-7) cells was processed and secreted as a mature-form into the culture medium. From the culture supernatant of ZPC-expressing transfectants cultured in the presence of ZPB1, both ZPB1 and ZPC were recovered as heterocomplexes by immunoprecipitation using either anti-HA or anti-ZPB1 antibody. Interestingly, a monoclonal antibody, 8E1, which immunoprecipitated free ZPB1, did not immunoprecipitate the ZPB1-ZPC heterocomplexes. An 8E1 epitope was mapped on a C-terminal region of the ZP domain in a ZPB1 molecule by identifying an 8E1-positive peptide using mass spectroscopy. Furthermore, by laser scanning confocal microscopy, ZPB1 and ZPC were observed to colocalize on the surface of ZPC-expressing transfectants cultured in the presence of ZPB1, whereas almost no ZPC was detected on the surface of the transfectants cultured in the absence of ZPB1. Taken together, these results suggest that ZPB1 transported into ovarian follicles encounters and associates with ZPC secreted from granulosa cells, resulting in the formation of heterocomplexes around an oocyte. In addition, it appears that such ZPB1-ZPC complexes accumulated on the oocyte surface act as a scaffold for subsequent matrix construction events including ZPX2 association.

    DOI: 10.1095/biolreprod.106.056267

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  230. Complex formation of 70-kDa heat shock protein with acidic glycolipids and phospholipids. Reviewed

    Harada Y, Sato C, Kitajima K.

    Biochem Biophys Res Commun.   Vol. 353   page: 655-660   2007

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  231. The rainbow trout CMP-sialic acid synthetase utilises a nuclear localization signal different from that identified in the mouse enzyme. Reviewed

    Tiralongo J, Fujita A, Sato C, Kitajima K, Lehmann F, Oschlies M, Gerardy-Schahn R, Munster-Kuhnel AK.

    Glycobiology   Vol. 17   page: 945-954   2007

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  232. Identification of an inflammation-inducible serum protein recognized by anti-disialic acid antibodies as carbonic anhydrase II. Reviewed

    Yasukawa, Z., Sato, C., and Kitajima, K.

    J Biochem.   Vol. 141   page: 429-441   2007

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    Acute phase proteins are an important marker of inflammation and sometimes have a role in the general defense response towards tissue injury. In the present study, we identified a 32 kDa protein that was immunoreactive with monoclonal antibody 2-4B (mAb.2-4B), which is specific to di/oligoNeu5Gc structures, and that behaved as an acute phase protein following stimulation with either turpentine oil or lipopolysaccharides. The 32 kDa protein was identified as carbonic anhydrase II (CA-II), based on matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analyses of the purified protein. Mouse and human CA-II was immunoreactive and immunoprecipitated with mAb.2-4B, but contained no sialic acid. In addition to mAb.2-4B, the mAb. S2-566 an antibody specific for diNeu5Ac-containing glycans, recognized the CA-II, whereas an anti-oligo/polysialic acid antibody did not. These results indicate that a part of the CA-II protein structure mimics the disialic acid structure recognized by the monoclonal antibodies. This is the first report that CA-II circulates in the serum following inflammation.

  233. シアル酸の構造多様性とその生物学的意義. Invited

    北島 健、郷 慎司、藤田 明子、佐藤ちひろ

    細胞工学   Vol. 26   page: 629-634   2007

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  234. Membrane microdomain formation is crucial in epiboly during gastrulation of medaka. Reviewed

    Adachi, T., Sato, C., and Kitajima, K.

    Biochem. Biophys. Res. Commun.   Vol. 355   page: 174-180   2007

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  235. Development of sensitive chemical and immunochemical methods for detecting sulfated sialic acids and their application to glycoconjugates from sea urchin sperm and eggs Reviewed

    Yamakawa, N., Sato C., Miyata, S., Maehashi, E., Toriyama, T., Sato, N., Furuhata, K., and Kitajima. K.

    Biochimie   Vol. 89   page: 1396-1408   2007

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  236. Glycobiology of polysialic acids on sea urchin gametes. Invited Reviewed

    Miyata, S., Sato, C., and Kitajima, K.

    Trends Glycosci. Glycotech.   Vol. 19   page: 83-95   2007

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  237. Hypoxia-enhanced expression of free deaminoneuraminic acid in human cancer cells. Reviewed

    Go, S., Sato, C., Yin, J., Kannagi, R., and Kitajima, K.

    Biochem. Biophys. Res. Commun.   Vol. 357   page: 537-542.   2007

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  238. Identification of the nuclear export signals that regulate the intracellular localization of the mouse CMP-sialic acid synthetase. Reviewed

    Fujita, A, Sato C, and Kitajima, K.

    Biochem. Biophys. Res. Commun.   Vol. 355   page: 174-180   2007

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  239. The rainbow trout CMP-sialic acid synthetase utilises a nuclear localization signal different from that identified in the mouse enzyme. Reviewed

    Tiralongo J, Fujita A, Sato C, Kitajima K, Lehmann F, Oschlies M, Gerardy-Schahn R, Munster-Kuhnel AK

    Glycobiology   Vol. 17   page: 945-954   2007

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  240. Hypoxia-enhanced expression of free deaminoneuraminic acid in human cancer cells. Reviewed

    Go, S, Sato, C, Yin, J, Kannagi, R, Kitajima, K

    Biochem. Biophys. Res. Commun.   Vol. 357   page: 537-542.   2007

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  241. Glycobiology of polysialic acids on sea urchin gametes. Invited Reviewed

    Miyata, S, Sato, C, Kitajima, K

    Trends Glycosci. Glycotech.   Vol. 19   page: 83-95   2007

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  242. Development of sensitive chemical and immunochemical methods for detecting sulfated sialic acids and their application to glycoconjugates from sea urchin sperm and eggs Reviewed

    Yamakawa, N, Sato C, Miyata, S, Maehashi, E, Toriyama, T, Sato, N, Furuhata, K, Kitajima. K

    Biochimie   Vol. 89   page: 1396-1408   2007

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  243. Complex formation of 70-kDa heat shock protein with acidic glycolipids and phospholipids. Reviewed

    Harada Y, Sato C, Kitajima K

    Biochem Biophys Res Commun.   Vol. 353   page: 655-660   2007

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  244. シアル酸の構造多様性とその生物学的意義. Invited

    北島 健, 郷 慎司, 藤田 明子, 佐藤ちひろ

    細胞工学   Vol. 26   page: 629-634   2007

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  245. Identification of the nuclear export signals that regulate the intracellular localization of the mouse CMP-sialic acid synthetase. Reviewed

    Fujita, A, Sato C, Kitajima, K

    Biochem. Biophys. Res. Commun.   Vol. 355   page: 174-180   2007

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  246. Membrane microdomain formation is crucial in epiboly during gastrulation of medaka. Reviewed

    Adachi, T, Sato, C, Kitajima, K

    Biochem. Biophys. Res. Commun.   Vol. 355   page: 174-180   2007

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  247. AMPAR removal underlies Abeta-induced synaptic depression and dendritic spine loss.

    Hsieh H, Boehm J, Sato C, Iwatsubo T, Tomita T, Sisodia S, Malinow R

    Neuron   Vol. 52 ( 5 ) page: 831-43   2006.12

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    DOI: 10.1016/j.neuron.2006.10.035

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  248. Expression and functional importance of the carbohydrate antigen 3D11 in early embryo of medaka, Oryzias latipes

    Kitajima Ken, Hara Hiroaki, Adachi Tomoko, Sato Chihiro

    ZOOLOGICAL SCIENCE   Vol. 23 ( 12 ) page: 1175-1175   2006.12

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  249. Flagellasialin: a novel sulfated alpha 2,9-linked polysialic acid glycoprotein of sea urchin sperm flagella Reviewed

    Shinji Miyata, Chihiro Sato, Hironobu Kumita, Masaru Toriyama, Victor D. Vacquier, Ken Kitajima

    GLYCOBIOLOGY   Vol. 16 ( 12 ) page: 1229 - 41   2006.12

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    A novel alpha 2,9-linked polysialic acid (polySia)-containing glycoprotein of sea urchin sperm flagella was identified and named "flagellasialin." Flagellasialin from Hemicentrotus pulcherrimus shows a diverse relative molecular mass on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of 40-80 kDa. Flagellasialin is a 96-amino acid, threonine-rich, heavily O-glycosylated (80-90% by weight) glycoprotein with a single transmembrane segment at its C-terminus and no apparent cytosolic domain. Of 12 extracellular Thr residues, eight are O-glycosylated and three are nonglycosylated. Flagellasialin is highly expressed in the testis but cannot be detected in the ovary. The amino acid sequences of flagellasialin from three sea urchin species (H. pulcherrimus, Strongylocentrotus purpuratus, and Strongylocentrotus franciscanus) are identical, but some species differences exist in the three core glycan structures to which the sulfated alpha 2,9-linked polyNeu5Ac chain is linked. Finally, the treatment of sperm with a specific antibody against the alpha 2,9-linked polyNeu5Ac structure results in the elevation of intracellular Ca2+ and inhibition of sperm motility and fertilization, implicating flagellasialin as a regulator of these critical processes.

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  250. Polysialic acid on flagellasialin is involved in sperm motility

    Miyata Shinji, Sato Chihiro, Kumita Hironobu, Toriyama Masaru, Kitajima Ken

    ZOOLOGICAL SCIENCE   Vol. 23 ( 12 ) page: 1171-1171   2006.12

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  251. Identification of disialic acid-containing glycoproteins in mouse serum: a novel modification of immunoglobulin light chains, vitronectin, and plasminogen Reviewed

    Z Yasukawa, C Sato, K Sano, H Ogawa, K Kitajima

    GLYCOBIOLOGY   Vol. 16 ( 7 ) page: 651 - 65   2006.7

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    Serum glycoproteins are involved in various biologic activities, such as the removal of exogenous antigens, fibrinolysis, and metal transport. Some of them are also useful markers of inflammation and disease. Although the amount of sialic acid increases following inflammation, little attention has been paid to the presence of linkage-specific epitopes in serum, especially the alpha 2,8-linkage. In a previous study, we demonstrated that four components in mouse serum contain alpha 2,8-linked disialic acid (diSia), based on immunoreactivity with monoclonal antibody 2-4B, which is specific to N-glycolyl-neuraminic acid (Neu5Gc)alpha 2 -&gt; (8Neu5Gc alpha 2 -&gt;)(n-1), n &gt;= 2 [Yasukawa et al., (2005) Glycobiology, 15, 827-837]. In this study, we purified three components, 30-, 70-, and 120-kDa gp, and identified them as an immunoglobulin (Ig) light chain, vitronectin, and plasminogen, respectively, using matrix-assisted laser desorption/ionization time-of-flight mass spectroscopy analyses. Modifications of these proteins with alpha 2,8-linked diSia were chemically confirmed by fluorometric C-7/C-9 analyses and mild acid hydrolysates-fluorometric anion-exchange chromatography analyses. We also demonstrated that the IgG, IgM, and IgE light chains are commonly modified with alpha 2,8-linked diSia. In addition, both mouse and rat vitronectin contained diSia, and the amount of disialylation in vitronectin dramatically decreased after hepatectomy. These results indicate that a novel diSia modification of serum glycoproteins is biologically important for immunologic events and fibrinolysis.

    DOI: 10.1093/glycob/cwj112

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  252. Hypoxic culture induces expression of Sialin, a sialic acid transporter, and cancer-associated gangliosides containing non-human sialic acid on human cancer cells. , Reviewed

    Yin, J., Hashimoto, A., Izawa, M., Miyazaki, K., Chen G-Y., Takematsu, H., Kozutsumi, Y., Suzuki, A., Furuhata, K., Cheng, F.-L. Lin, C.-H., Sato, C., Kitajima, K., and Kannagi, R.

    Cancer Res.   Vol. 66   page: 2937-2945   2006

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  253. Flagellasialin: a novel sulfated alpha2,9-linked polysialic acid glycoprotein of sea urchin sperm flagella. Reviewed

    Miyata S, Sato C, Kumita H, Toriyama M, Vacquier VD, Kitajima K.

    Glycobiology   Vol. 16   page: 1229-12241   2006

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    A novel α2,9-linked polysialic acid-containing glycoprotein of sea urchin sperm flagella was previously identified and named “flagellasialin". Flagellasialin from Hemicentrotus pulcherrimus shows a diverse relative molecular mass on SDS-PAGE of 40-80 kDa. Its glycan structures were determined as HSO3→8Neu5Acα2→9(Neu5Acα2→9)n-2Neu5Acα2→6GalNAcα1→Thr (n, averaging 15) [Miyata et al. (2004) Glycobiology, 14, 827-840]. In this study, we purified flagellasialin, and cloned its cDNA. Flagellasialin is a 96-amino acid, threonine-rich, heavily O-glycosylated (80-90% by weight) glycoprotein with a single transmembrane segment at its C-terminus and no apparent cytosolic domain. Of 12 extracellular Thr residues, eight are O-glycosylated and three are non-glycosylated. Flagellasialin is highly expressed in testis, but cannot be detected in ovary. The amino acid sequences of flagellasialin from three sea urchin species (H. pulcherrimus, Strongylocentrotus purpuratus and S. franciscanus) are identical, but some species differences exist in the three core glycan structures to which the sulfated α2,9-linked polyNeu5Ac chain is linked. Finally, a specific antibody against the α2,9-linked polyNeu5Ac structure inhibits sperm motility and reduces fertilization, implicating flagellasialin as a regulator of these critical processes.

  254. *Identification of disialic acid-containing glycoproteins in mouse serum. Reviewed

    Yasukawa, Z., Sato, C., Sano, K., Ogawa, H. and Kitajima, K.

    Glycobiology   Vol. 15   page: 827-837   2006

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    Serum glycoproteins are involved in various biological activities such as removal of exogenous antigen, fibrinolysis, transport of metal. They are also useful markers of inflammation and diseases. Although it is known that the amount of sialic acid increases after inflammation, little attention has been paid to the presence of linkage specific epitope in serum,especially, the α2,8-linkage. In the previous study, we demonstrated that four components in mouse serum contain the α2,8-linked disialic acid, based on the immunoreactivity with mAb.2-4B that is specific to Neu5Gcα2→(8Neu5Gcα2→)n-1, n≥2 (Yasukawa, Z. et al. (2005) Glycobiology 15, 827-837). In this study, we purified three components, 30kDa-gp, 70kDa-gp, and 120kDa-gp and identified as the light chain of immunoglobulins, vitronectin, and plasminogen, respectively, using MALDI-TOF MS analyses. The modifications of these proteins with the α2,8-linked disialic acid was chemically confirmed by fluorometric C7/C9 analyses and acid hydrolysates-DMB methods. We also showed that the light chains of IgG, IgM and IgE are commonly modified with the α2,8-linked disialic acid. In addition, not only mouse vitronectin but also rat vitronectin was also modified with disialic acid and the amount of disialylation on vitronectin decreased after hepatectomy. These results indicate that disialic acid-modification on serum glycoprotein has some biological meanings in immunological events and fibrinolysis.

  255. Involvement of the alpha2,8-polysialyltransferases II/STX and IV/PST in the biosynthesis of polysialic acid chains on the O-linked glycoproteins in rainbow trout ovary. Reviewed

    Asahina S, Sato C, Matsuno M, Matsuda T, Colley K, Kitajima K. Asahina S, Sato C, Matsuno M, Matsuda T, Colley K, Kitajima K.

    J Biochem.   Vol. 140   page: 687-701   2006

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  256. Oral ingestion of mannose alters the expression level of deaminoneuraminic acid (KDN) in mouse organs. Reviewed

    Go, S., Sato, C., and Kitajima, K.

    Glycoconjugate J.   Vol. 23   page: 411-421   2006

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  257. Weaning-induced expression of a milk-fat globule protein, MFG-E8, in mouse mammary glands, as demonstrated by the analyses of its mRNA, protein and phosphatidylserine-binding activity. Reviewed

    Nakatani H, Aoki N, Nakagawa Y, Jin-No S, Aoyama K, Oshima K, Ohira S, Sato C, Nadano D, Matsuda T.

    Biochem J.   Vol. 395   page: 21-30   2006

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  258. Weaning-induced expression of a milk-fat globule protein, MFG-E8, in mouse mammary glands, as demonstrated by the analyses of its mRNA, protein and phosphatidylserine-binding activity. Reviewed

    Nakatani H, Aoki N, Nakagawa Y, Jin-No S, Aoyama K, Oshima K, Ohira S, Sato C, Nadano D, Matsuda T

    Biochem J.   Vol. 395   page: 21-30   2006

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  259. Hypoxic culture induces expression of Sialin, a sialic acid transporter, and cancer-associated gangliosides containing non-human sialic acid on human cancer cells. , Reviewed

    Yin, J, Hashimoto, A, Izawa, M, Miyazaki, K, Chen G-Y, Takematsu, H, Kozutsumi, Y, Suzuki, A, Furuhata, K, Cheng, F.-L. Lin, C.-H, Sato, C, Kitajima, K, Kannagi, R

    Cancer Res.   Vol. 66   page: 2937-2945   2006

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  260. Involvement of the alpha2,8-polysialyltransferases II/STX and IV/PST in the biosynthesis of polysialic acid chains on the O-linked glycoproteins in rainbow trout ovary. Reviewed

    Asahina S, Sato C, Matsuno M, Matsuda T, Colley K, Kitajima, K, Asahina S, Sato C, Matsuno M, Matsuda T, Colley K, Kitajima K

    J Biochem.   Vol. 140   page: 687-701   2006

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  261. Oral ingestion of mannose alters the expression level of deaminoneuraminic acid (KDN) in mouse organs. Reviewed

    Go, S, Sato, C, Kitajima, K

    Glycoconjugate J.   Vol. 23   page: 411-421   2006

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  262. An N-terminal 130 kDa fragment of a fourth sea urchin sperm receptor for egg jelly module protein, REJ4, is secreted during acrosome reaction

    Miyata Shinji, Sato Chihiro, Toriyama Masaru, Kitajama Ken

    ZOOLOGICAL SCIENCE   Vol. 22 ( 12 ) page: 1456-1456   2005.12

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  263. Capacitation-induced changes of the surface distribution of sialoglycotopes on pig sperm

    Xue Lian, Miyata Shinji, Sato Chihiro, Kitajima Ken

    ZOOLOGICAL SCIENCE   Vol. 22 ( 12 ) page: 1456-1456   2005.12

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  264. Inflammation-dependent changes in alpha 2,3-, alpha 2,6-, and alpha 2,8-sialic acid glycotopes on serum glycoproteins in mice Reviewed

    Z Yasukawa, C Sato, K Kitajima

    GLYCOBIOLOGY   Vol. 15 ( 9 ) page: 827 - 37   2005.9

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    The expression of acute-phase serum proteins increases in response to inflammatory stimuli. Most of these proteins are glycoproteins that often contain sialic acids (Sia). It is unknown, however, how the expression of Sia in these glycoproteins changes during inflammation. This study demonstrates changes in the alpha 2,3-, alpha 2,6-, and alpha 2,8-Sia glycotopes on serum glycoproteins in response to turpentine oil-induced inflammation, based on lectin- and immunoblot analyses by using sialyl linkage-specific lectins, Maackia amurensis for the alpha 2,3-Sia glycotope and Sambucus sieboldiana for the alpha 2,6-Sia glycotopes, and monoclonal antibody 2413 (mAb.2-413) recognizing the di- and oligomers of the alpha 2,8-Neu5Gc residue. There was an increase in a limited number of sialoglycoproteins containing the alpha 2,3-, alpha 2,6-, or alpha 2,8-Sia glycotopes. Reverse transcription-polymerase chain reaction (RT-PCR) analysis of the expression profiles of mRNAs for the known sialyltransferases in mouse liver during inflammation indicated the up-regulated expression of P-galactoside alpha 2,3-sialyltransferases (ST3Gal I and ST3Gal III) and P-N-acetylgalactosaminide alpha 2,6-sialyltransferase (ST6GalNAc VI) as well as P-galactoside alpha 2,6-sialyltransferase (ST6Gal 1) mRNAs. Notably, ST3Gal I and III and ST6GalNAc VI are involved in the synthesis of the alpha 2,3- and alpha 2,6-Sia glycotopes on O-glycan chains and possibly on gangliosides, whereas ST6Gal I is specific for N-glycan chains. These results provide evidence for the inflammation-induced expression of sialyl glycotopes in serum glycoproteins. We demonstrated that inflammation significantly increased the expression of an unknown 32-kDa glycoprotein containing the alpha 2,8-Sia glycotope. The mechanism for the increase in glycoprotein in inflamed mouse serum remains to be examined, as mRNA expression for all of the alpha 2,8-sialyltransferases (ST8Sia I-VI) was unchanged during inflammation.

    DOI: 10.1093/glycob/cwi068

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  265. Crystallization and preliminary X-ray analysis of mitochondrial presequence receptor Tom20 in complexes with a presequence from aldehyde dehydrogenase

    Igura M, Ose T, Obita T, Sato C, Maenaka K, Endo T, Kohda D

    ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS   Vol. 61 ( 5 ) page: 514-517   2005.5

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    DOI: 10.1107/S1744309105011577

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  266. Distal myopathy with rimmed vacuoles - Impaired O-glycan formation in muscular glycoproteins

    Tajima Y, Uyama E, Go S, Sato C, Tao N, Kotani M, Hino H, Suzuki A, Sanai Y, Kitajima K, Sakuraba H

    AMERICAN JOURNAL OF PATHOLOGY   Vol. 166 ( 4 ) page: 1121-1130   2005.4

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  267. Distal myopathy with rimmed vacuoles - Impaired O-glycan formation in muscular glycoproteins Reviewed

    Y Tajima, E Uyama, S Go, C Sato, N Tao, M Kotani, H Hino, A Suzuki, Y Sanai, K Kitajima, H Sakuraba

    AMERICAN JOURNAL OF PATHOLOGY   Vol. 166 ( 4 ) page: 1121 - 30   2005.4

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    Distal myopathy with rimmed vacuoles (DMRV), is an autosomal recessive disorder with early adult onset, displays distal dominant muscular involvement and is characterized by the presence of numerous rimmed vacuoles in the affected muscle fibers. The pathophysiology of DMRV has not been clarified yet, although the responsible gene was identified as that encoding UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase involved in the biosynthesis of sialic acids. To identify defective carbohydrate moieties of muscular glycoproteins; from DMRV patients, frozen skeletal muscle sections from seven patients with DMRV, as well as normal and pathological controls, were treated with or without sialidase or N-glycosidase F followed by lectin staining and lectin blotting analysis. The sialic acid contents of the O-glycans in the skeletal muscle specimens from the DMRV patients were also measured. We found that Aracbis hypogaea agglutinin (PNA) lectin reacted strongly with sarcolemmal glycoproteins in the DMRV patients but not with those in control subjects. a-Dystroglycan from the DMRV patients strongly associated with PNA lectin, although that from controls did not. The sialic acid level of the O-glycans in the DMRV muscular glycoproteins with molecular weights of 30 to 200 kd was reduced to 60 to 80% of the control level. The results show that impaired sialyl O-glycan formation in muscular glycoproteins, including a-dystroglycan, occurs in DMRV.

    DOI: 10.1016/S0002-9440(10)62332-2

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  268. Differentiation of noncancerous tissue and cancer lesions by apparent diffusion coefficient values in transition and peripheral zones of the prostate

    Sato C, Naganawa S, Nakamura T, Kumada H, Miura S, Takizawa O, Ishigaki T

    JOURNAL OF MAGNETIC RESONANCE IMAGING   Vol. 21 ( 3 ) page: 258-262   2005.3

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    DOI: 10.1002/jmri.20251

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  269. Development of a simple and efficient method for assaying cytidine monophosphate sialic acid synthetase activity using an enzymatic reduced nicotinamide adenine dinucleotide/oxidized nicotinamide adenine dinucleotide converting system Reviewed

    A Fujita, C Sato, AK Munster-Kuhnel, R Gerardy-Schahn, K Kitajima

    ANALYTICAL BIOCHEMISTRY   Vol. 337 ( 1 ) page: 12 - 21   2005.2

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    A new reliable method to assay the activity of cytidine monophosphate sialic acid (CMP-Sia) synthetase (CSS) has been developed. The activation of sialic acids (Sia) to CMP-Sia is a prerequisite for the de novo synthesis of sialoglycoconjugates. In vertebrates, CSS has been cloned from human, mouse, and rainbow trout, and the crystal structure has been resolved for the mouse enzyme. The mouse and rainbow trout enzyme have been compared with respect to substrate specificity, demonstrating that the mouse enzyme exhibits a pronounced specificity for N-acetylneuraminic acid (Neu5Ac), while the rainbow trout CSS is equally active with either of three Sia species, Neu5Ac, N-glycolylneuraminic acid (Neu5Gc), and deaminoncuraminic acid (KDN). However, molecular details that explain the pronounced substrate specificities are unknown. Understanding the catalytic mechanisms of these enzymes is of major importance, since CSSs play crucial roles in cellular sialylation patterns and thus are potential drug targets in a number of pathophysiological situations. The availability of the cDNAs and the obtained structural data enable rational approaches; however, these efforts are limited by the lack of a reliable high-throughput assay system. Here we describe a new assay system that allows product quantification in a reduced nicotinamide adenine dinucleotide (NADH)-dependent color reaction. The activation reaction catalyzed by CSS, CTP + Sia --&gt; CMP-Sia + pyrophosphate, was evaluated by a consumption of Sia, which corresponds to that of NADH on the following two successive reactions: (i) Sia --&gt; pyruvate + ManNAc (or Man), catalyzed by a sialic acid lyase (SAL), and (ii) pyruvate + NADH --&gt; lactate + oxidized nicotinamide adenine dinucleotide (NAD+), catalyzed by a lactate dehydrogenase (LDH). Consumption of NADH can be photometrically monitored on a microtiter plate reader for a number of test samples at the same time. Furthermore, based on the quantification of CSS used in the SAL/LDH assay, relative activities toward Sia derivatives have been obtained. The preference of mouse CSS toward Neu5Ac and the ability of the rainbow trout enzyme to activate both KDN and Neu5Ac were confirmed. Thus, this simple and time-saving method is suitable for a systematic comparison of enzyme activity of structurally mutated enzymes based on the relative specific activity. (C) 2004 Elsevier Inc. All rights reserved.

    DOI: 10.1016/j.ab.2004.10.033

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  270. Distal Myopathy with Rimmed Vaculoes. Reviewed

    Tajima, Y., Uyama, E., Go, S., Sato, C., Tao, N., Kotani, M., Hino, H., Suzuki, A.,Sanai, Y., Kitajima, K., and Sakuraba, H.

    Am. J. Pathol.   Vol. 166   page: 1121-1128   2005

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  271. Inflammation-dependent changes in α2,3-, α2,6-, and α2,8-sialic acid glycotopes on serum glycoproteins in mouse. Reviewed

    Yasukawa, Z., Sato, C., and Kitajima, K.

    Glycobiology   Vol. 15   page: 827-837   2005

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  272. A major ganglioside from Hemicentrotus pulcherrimus sperm specifically interacts with phospholipids

    Yamakawa Nao, Sato Chihiro, Kitajima Ken

    ZOOLOGICAL SCIENCE   Vol. 21 ( 12 ) page: 1292-1292   2004.12

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  273. Cell growth stimulating activity of the peptides derived from cortical alveolus glycoproteins of fish egg (hyosophorin)

    Maeda Eri, Sato Chihiro, Kitajima Ken

    ZOOLOGICAL SCIENCE   Vol. 21 ( 12 ) page: 1292-1292   2004.12

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  274. The 130 kDa glycoprotein is secreted from sperm during acrosome reaction of sea urchin, Hemicentrotus pulcherrimus

    Miyata Shinji, Sato Chihiro, Toriyama Masaru, Hirohashi Noritaka, Vacquier Victor, Kitajima Ken

    ZOOLOGICAL SCIENCE   Vol. 21 ( 12 ) page: 1292-1292   2004.12

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  275. Development of sensitive chemical and immunochemical methods for detecting sulfated sialic acids and their application to localization and quantitation studies of sea urchin sperm and eggs

    Yamakawa N, Maehashi E, Miyata S, Sato C, Furuhata K, Kitajima K

    GLYCOBIOLOGY   Vol. 14 ( 11 ) page: 1146-1146   2004.11

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  276. Effects of exogenously administered mannose and deaminoneuraminic acid (KDN) to mice or murine cultured cells on the metabolism of KDN

    Go S, Sato C, Kitajima K

    GLYCOBIOLOGY   Vol. 14 ( 11 ) page: 1166-1167   2004.11

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  277. Mouse heat shock protein 70 recognizes negatively charged glycolipids and phospholipids

    Harada Y, Sato C, Kitajima K

    GLYCOBIOLOGY   Vol. 14 ( 11 ) page: 1101-1102   2004.11

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  278. Studies on chemical properties of cyclic sialic acid using their synthetic S- and O-glycosides as model compounds

    Iwata S, Sato C, Ando H, Kiso M, Kannagi R, Kitajima K

    GLYCOBIOLOGY   Vol. 14 ( 11 ) page: 1163-1163   2004.11

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  279. Occurrence and characterization of a novel sulfated alpha 2,9-linked polysialic acid-containing glycoprotein in sea urchin sperm flagellum

    Miyata S, Sato C, Kitajima K

    GLYCOBIOLOGY   Vol. 14 ( 11 ) page: 1146-1146   2004.11

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  280. Mouse T cell CD166 is a disialic acid-containing glycoprotein and involved in T cell activation

    Sato C, Nohara K, Matsuda T, Kitajima K

    GLYCOBIOLOGY   Vol. 14 ( 11 ) page: 1136-1136   2004.11

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  281. A major flagellum sialoglycoprotein in sea urchin sperm contains a novel polysialic acid, an alpha 2,9-linked poly-N-acetyineuraminic acid chain, capped by an 8-O-sulfated sialic acid residue Reviewed

    S Miyata, C Sato, S Kitamura, M Toriyama, K Kitajima

    GLYCOBIOLOGY   Vol. 14 ( 9 ) page: 827 - 40   2004.9

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    A new type of polysialic acid (polySia) structure was demonstrated to occur in a major unknown sialoglycoprotein with a diverse molecular mass of 40-80 kDa in sea urchin sperm. The polySia-containing glycan structure was determined to be HSO3--&gt;8Neu5Acalpha2--&gt;9(Neu5Acalpha2--&gt;9)(n-2) Neu5Acalpha2--&gt;6GalNAcalpha1--&gt;Ser/Thr (n, on average 15), based on carbohydrate analysis of the sialoglycopeptide obtained by an exhaustive protease digestion of whole sperm, fluorometric anion-exchange high-performance liquid chromatography, and methylation analysis. The sulfate group was predominantly localized to the nonreducing terminus of the polySia chain. This is the first example of an alpha2,9-linked polySia structure in animal sperm. The polySia-containing sialoglycoprotein was present in sperm flagellum but not in the head. Furthermore, this sialoglycoprotein localized in the sperm lipid raft, which contains an enriched ganglioside (Neu5Acalpha2--&gt;8Neu5Acalpha2--&gt;6GlcCer), a receptor for sperm-activating peptide (speract), and its associated guanylate cyclase (Ohta et al. [2000] Glycoconj. J., 17, 205-214).

    DOI: 10.1093/glycob/cwh100

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  282. Chain length diversity of sialic acids and its biological significance

    Sato C

    TRENDS IN GLYCOSCIENCE AND GLYCOTECHNOLOGY   Vol. 16 ( 91 ) page: 331-344   2004.9

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  283. 母乳中に発見された新規ポリシアロ糖タンパク質 : CD36がユニークな糖鎖修飾を受けていることが明らかに

    佐藤 ちひろ

    化学と生物   Vol. 42 ( 1 ) page: 12-14   2004.1

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  284. alpha-C-Mannosyltryptophan is not recognized by conventional mannose-binding lectins Reviewed

    T.Nishikawa, S. Kajii, C. Sato, Z. Yasukawa, K. Kitajima, and M. Isobe

    Bioorg Med Chem.     page: 2343-2348   2004

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  285. A major flagellum sialoglycoprotein in sea urchin sperm contains a novel polysialic acid, an α2,9-linked poly-N-acetylneuraminic acid chain, capped by an 8-O-sulfated sialic acid residue Reviewed

    S. Miyata, C. Sato, S. Kitamura, S. Toriyama, and K. Kitajima,

    Glycobiology   Vol. 14   page: 827-840   2004

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    A new type of polysialic acid (polySia) structure was demonstrated to occur in a major unknown sialoglycoprotein with a diverse molecular mass of 40-80 kDa in sea urchin sperm. The polySia-containing glycan structure was determined to be HSO3→8Neu5Acα2→9(Neu5Acα2→9)n-2 Neu5Acα2 →6GalNAcα1→Ser/Thr (n, on average 15), based on carbohydrate analysis of the sialoglycopeptide obtained by an exhaustive protease digestion of whole sperm, fluorometric anion-exchange high performance liquid chromatography, and methylation analysis. role in sperm activation and migration in the extracellular coats at fertilization, like polySia on neural cell adhesion molecule acts as a regulator of neural cell functions.

  286. Specificity of human trans-sialidase as probed with gangliosides. Reviewed

    Nikonova, E. Y., Tertov, V. V., Sato, C., Kitajima, K., and Bovin, N. V.

    Bioorg. Med. Chem. Lett.   Vol. 14   page: 5161-5164   2004

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  287. A newly identified zona pellucida glycoprotein, ZPD and dimeric ZP1 of chicken egg envelope are involved in sperm activation on sperm-egg interaction Reviewed

    H. Okumura, Y. Kohno, Y. Iwata, H. Mori, N. Aoki, C. Sato, K. Kitajima, D. Nadano, and T. Matsuda

    Biochem. J.   Vol. 383   page: 1-9   2004

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  288. Development of a simple and efficient method for assaying the CMP-sialic acid synthetase activity using an enzymatic NADH/NAD+ converting system Reviewed

    A. Fujita, C. Sato, A.-K. Münster-Kühnel, R. Gerardy-Schahn, and K. Kitajima

    Anal. Biochem.   Vol. 337   page: 12-21   2004

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  289. *Chain length diversity of sialic acids and its biological significance (Review) Invited Reviewed

    C. Sato

    Trends Glycosci. Glycotechnol   Vol. 16   page: 331-344   2004

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  290. Developmental Expression of a Sialyltransferase Responsible for Sialylation of Cortical Alveolus Glycoprotein During Oogenesis in Rainbow Trout (Oncorhynchus mykiss) Reviewed

    S. Asahina, C.Sato, and K. Kitajima

    J. Biochem.   Vol. 136   page: 189-198   2004

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  291. ジシアル酸・オリゴシアル酸・ポリシアル酸の生物学的機能 Invited

    佐藤ちひろ

      Vol. 3   page: 40-47   2004

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  292. Specificity of human trans-sialidase as probed with gangliosides. Reviewed

    Nikonova, E. Y, Tertov, V. V, Sato, C, Kitajima, K, Bovin, N. V

    Bioorg. Med. Chem. Lett.   Vol. 14   page: 5161-5164   2004

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  293. ジシアル酸・オリゴシアル酸・ポリシアル酸の生物学的機能 Invited

    佐藤ちひろ

    Functional Glycomics   Vol. 3   page: 40-47   2004

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  294. Developmental Expression of a Sialyltransferase Responsible for Sialylation of Cortical Alveolus Glycoprotein During Oogenesis in Rainbow Trout (Oncorhynchus mykiss) Reviewed

    S. Asahina, C.Sato, K. Kitajima

    J. Biochem.   Vol. 136   page: 189-198   2004

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  295. alpha-C-Mannosyltryptophan is not recognized by conventional mannose-binding lectins Reviewed

    T.Nishikawa, S. Kajii, C. Sato, Z. Yasukawa, K. Kitajima, M. Isobe

    Bioorg Med Chem.     page: 2343-2348   2004

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  296. A newly identified zona pellucida glycoprotein, ZPD and dimeric ZP1 of chicken egg envelope are involved in sperm activation on sperm-egg interaction Reviewed

    H. Okumura, Y. Kohno, Y. Iwata, H. Mori, N. Aoki, C. Sato, K. Kitajima, D. Nadano, T. Matsuda

    Biochem. J.   Vol. 383   page: 1-9   2004

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  297. *Chain length diversity of sialic acids and its biological significance (Review) Invited Reviewed

    C. Sato

    Trends Glycosci. Glycotechnol   Vol. 16   page: 331-344   2004

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  298. Frequent occurrence and biological significance of degree of polymerization in sialic acid

    SATO Chihiro

      Vol. 75 ( 12 ) page: 1526-1530   2003.12

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  299. [Frequent occurrence and biological significance of degree of polymerization in sialic acid]. Reviewed

    Sato C

    Seikagaku. The Journal of Japanese Biochemical Society   Vol. 75 ( 12 ) page: 1526 - 1530   2003.12

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  300. Frequent occurrence and biological significance of degree of polymerization in sialic acid

    Sato C.

    Seikagaku. The Journal of Japanese Biochemical Society   Vol. 75 ( 12 ) page: 1526-1530   2003.12

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  301. Frequent occurrence and biological significance of degree of polymerization in sialic acid

    Sato C

    SEIKAGAKU   Vol. 75 ( 12 ) page: 1526-1530   2003.12

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  302. Discovery of two new polysialoglycoproteins in human and sea urchin. Linkage and length diversities of polysialic acid in animal glycoproteins

    Kitajima K, Yabe U, Miyata S, Sato CH

    GLYCOBIOLOGY   Vol. 13 ( 11 ) page: 844-844   2003.11

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  303. Identification of the sea urchin 350-kDa sperm-binding protein as a new sialic acid-binding lectin that belongs to the heat shock protein 110 family - Implication of its binding to gangliosides in sperm lipid rafts in fertilization Reviewed

    E Maehashi, C Sato, K Ohta, Y Harada, T Matsuda, N Hirohashi, WJ Lennarz, K Kitajima

    JOURNAL OF BIOLOGICAL CHEMISTRY   Vol. 278 ( 43 ) page: 42050 - 7   2003.10

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    The 350-kDa sperm-binding protein (SBP), a species-specific sperm-binding protein, is localized in the vitelline layer of sea urchin eggs. In this study, we have shown for the first time that sperm gangliosides are ligands for the intact glycosylated SBP. Using recombinant fragments of the SBP, the N-terminal heat shock protein 110-like domain was shown to be responsible for the binding. The intact SBP could bind various gangliosides, and the binding was sialidase-sensitive and inhibited by sialyllactose, thus indicating that it is the sialic acid-binding protein. Calcium and magnesium ions were not required but they did enhance the binding activity of SBP. The observation that bacterially expressed recombinant SBP and the sialidase-treated intact glycosylated SBP lost divalent cation-dependent enhancement of binding activity suggests that the sialylated carbohydrate moieties of the SBP may be involved in this property. Furthermore, the SBP was shown to bind sperm lipid rafts, in which gangliosides are enriched, and this binding was lost upon sialidase treatment of the lipid rafts. Finally, liposomes containing the ganglioside specifically inhibited fertilization. Taken together, these results allow us to identify SBP as a member of a new class of sialic acid-binding lectin belonging to the Hsp110 family, and indicate that SBP may be involved in interaction of sperm with the vitelline layer of the egg.

    DOI: 10.1074/jbc.M307493200

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  304. [Oligosialic acid: a common carbohydrate epitope in glycoproteins and glycolipids]. Reviewed

    Sato C, Kitajima K

    Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme   Vol. 48 ( 12 ) page: 1730 - 1736   2003.9

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  305. Polysialic acid in human milk - CD36 is a new member of mammalian polysialic acid-containing glycoprotein Reviewed

    U Yabe, C Sato, T Matsuda, K Kitajima

    JOURNAL OF BIOLOGICAL CHEMISTRY   Vol. 278 ( 16 ) page: 13875 - 80   2003.4

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    The neural cell adhesion molecule and the voltage-sensitive sodium channel a-subunit are the only two molecules in mammals known to be modified by alpha-2,8-linked polysialic acid (polySia). We found a new polySia-containing glycoprotein in human milk and identified it as CD36, a member of the B class of the scavenger receptor superfamily. The polySia-containing glycan chain(s) were removed by alkaline treatment but not by peptide: N-glycanase F digestion, indicating that milk CD36 contained polySia on O-linked glycan chain(s). Polysialylation of CD36 occurs not only in human milk but also in mouse milk. However, CD36 in human platelets is not polysialylated. PolySia CD36 is secreted in milk at any lactation stage and reaches peak level at 1 month after parturition. Thus, it is suggested that polySia of milk CD36 is significant for neonatal development in terms of protection and nutrition.

    DOI: 10.1074/jbc.M300458200

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  306. Polysialic acid in human milk Reviewed

    U. Yabe, C. Sato, T. Matsuda, and K. Kitajima

    J. Biol. Chem.   Vol. 278   page: 13875-13880   2003

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  307. ポリシアル酸の鎖長多様性とタンパク質機能 Invited Reviewed

    佐藤ちひろ

    生化学   Vol. 75   page: 1526-1530   2003

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  308. 糖タンパク質と糖脂質の共通糖鎖オリゴシアル酸の普遍的存在とその意義 Invited

    佐藤ちひろ、北島 健

    蛋白質・核酸・酵素   Vol. 48   page: 1730-1736   2003

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  309. Identification of the sea urchin 350 kDa sperm binding protein as a new sialic acid-binding lectin that belongs to the heat shock protein 110 family. Reviewed

    E. Maehashi, C. Sato, K. Ohta, Y. Harada, T., Matsuda, N. Hirohashi, W. J. Lennarz, and K. Kitajima

    J. Biol. Chem.   Vol. 278   page: 42050-42057   2003

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  310. ポリシアル酸の鎖長多様性とタンパク質機能 Invited Reviewed

    佐藤ちひろ

    生化学   Vol. 75   page: 1526-1530   2003

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  311. 糖タンパク質と糖脂質の共通糖鎖オリゴシアル酸の普遍的存在とその意義 Invited

    佐藤ちひろ, 北島 健

    蛋白質・核酸・酵素   Vol. 48   page: 1730-1736   2003

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  312. マウス炎症時における血清糖タンパク質上のシアル酸構造の変動

    安川 然太, 佐藤 ちひろ, 松田 幹, 北島 健

    生化学   Vol. 74 ( 11 ) page: 1389 - 1389   2002.11

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  313. 炎症誘導マウスにおけるジ及びオリゴシアル酸含有血清糖タンパク質の解析

    安川 然太, 佐藤 ちひろ, 北島 健

    生化学   Vol. 74 ( 8 ) page: 726 - 726   2002.8

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  314. *Neuronal differentiation-dependent expression of the disialic acid epitope on CD166 and its involvement in neurite formation in Neuro2A cells. Reviewed

    C. Sato, T. Matsuda, and K. Kitajima

    J. Biol. Chem.   Vol. 277   page: 45299-45303   2002

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  315. *Neuronal differentiation-dependent expression of the disialic acid epitope on CD166 and its involvement in neurite formation in Neuro2A cells. Reviewed

    C. Sato, T. Matsuda, K. Kitajima

    J. Biol. Chem.   Vol. 277   page: 45299-45303   2002

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  316. Occurrence of oligosialic acids on integrin alpha(5) subunit and their involvement in cell adhesion to fibronectin

    Nadanaka S, Sato C, Kitajima K, Katagiri K, Irie S, Yamagata T

    JOURNAL OF BIOLOGICAL CHEMISTRY   Vol. 276 ( 36 ) page: 33657-33664   2001.9

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  317. Identification and adipocyte differentiation-dependent expression of the unique disialic acid residue in an adipose tissue-specific glycoprotein, adipo Q

    Sato C, Yasukawa Z, Honda N, Matsuda T, Kitajima K

    JOURNAL OF BIOLOGICAL CHEMISTRY   Vol. 276 ( 31 ) page: 28849-28856   2001.8

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  318. 脂肪細胞特異的血清タンパク質adipo Qのジ/オリゴシアル酸含有糖鎖の構造

    安川 然太, 佐藤 ちひろ, 本田 直輝, 松田 幹, 北島 健

    生化学   Vol. 73 ( 8 ) page: 695 - 695   2001.8

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  319. Identification and adipocyte differentiation-dependent expression of the unique disialic acid residues in an adipose tissue specific glycoprotein, adipo Q Reviewed

    "C. Sato, Z. Yasukawa, N. Honda, T. Matsuda, and K. Kitajima"

    J. Biol. Chem.   Vol. 276   page: 28849-28856   2001.1

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  320. Occurrence of oligosialic acids on integrin a5 subunit and their involvement in cell adhesion to fibronectin. Reviewed

    "S. Nadanaka, C. Sato, K. Kitajima, K. Katagiri, S. Irie, and T. Yamagata 276 (2001) in press"

    J. Biol. Chem.   Vol. 276   page: 33657-33664   2001.1

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  321. Identification and adipocyte differentiation-dependent expression of the unique disialic acid residues in an adipose tissue specific glycoprotein, adipo Q Reviewed

    C. Sato, Z. Yasukawa, N. Honda, T. Matsuda, K. Kitajima

    J. Biol. Chem.   Vol. 276   page: 28849-28856   2001.1

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  322. Occurrence of oligosialic acids on integrin a5 subunit and their involvement in cell adhesion to fibronectin. Reviewed

    S. Nadanaka, C. Sato, K. Kitajima, K. Katagiri, S. Irie, T. Yamagat, in press

    J. Biol. Chem.   Vol. 276   page: 33657-33664   2001.1

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  323. ウシ胎児血清糖タンパク質adipo Qの精製とそのジシアル酸含有糖鎖の同定

    安川 然太, 佐藤 ちひろ, 本田 直輝, 松田 幹, 北島 健

    生化学   Vol. 72 ( 8 ) page: 719 - 719   2000.8

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  324. Frequent occurrence of pre-existing alpha 2 -> 8-linked disialic and oligosialic acids with chain lengths up to 7 Sia residues in mammalian brain glycoproteins - Prevalence revealed by highly sensitive chemical methods and anti-di-, oligo-, and poly-Sia antibodies specific for defined chain lengths

    Sato C, Fukuoka H, Ohta K, Matsuda T, Koshino R, Kobayashi K, Troy FA, Kitajima K

    JOURNAL OF BIOLOGICAL CHEMISTRY   Vol. 275 ( 20 ) page: 15422-15431   2000.5

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    Web of Science

  325. Co-localization of receptor and transducer proteins in the glycosphingolipid-enriched, low density, detergent-insoluble membrane fraction of sea urchin sperm. Reviewed

    "K. Ohta,, C. Sato, T. Matsuda, M. Toriyama, V. D. Vacquier, W. J.Lennarz, and K. Kitajima"

    Glycoconjugate J.   Vol. 17   page: 205-214   2000.1

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  326. *Frequent occurrence of pre-existing alpha2,8-linked disialic and oligosialic acids with chain lengths up to 7 Sia residues in mammalian brain glycoproteins. Reviewed

    "C. Sato, H. Fukuoka, K. Ohta, T. Matsuda, R. Koshino, K. Kobayashi, F. A. Troy II, and K. Kitajima"

    J. Biol. Chem.   Vol. 275 (20)   page: 15422-15431   2000.1

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  327. *Frequent occurrence of pre-existing alpha2,8-linked disialic and oligosialic acids with chain lengths up to 7 Sia residues in mammalian brain glycoproteins. Reviewed

    C. Sato, H. Fukuoka, K. Ohta, T. Matsuda, R. Koshino, K. Kobayashi, F. A. Troy II, K. Kitajima

    J. Biol. Chem.   Vol. 275 (20)   page: 15422-15431   2000.1

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  328. Co-localization of receptor and transducer proteins in the glycosphingolipid-enriched, low density, detergent-insoluble membrane fraction of sea urchin sperm. Reviewed

    K. Ohta, C. Sato, T. Matsuda, M. Toriyama, V. D. Vacquier, W. J.Lennarz, K. Kitajima

    Glycoconjugate J.   Vol. 17   page: 205-214   2000.1

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  329. Co-localization of receptor and transducer proteins in the glycosphingolipid-enriched, low density, detergent-insoluble membrane fraction of sea urchin sperm

    Ohta K, Sato C, Matsuda T, Toriyama M, Vacquier VD, Lennarz WJ, Kitajima K

    GLYCOCONJUGATE JOURNAL   Vol. 17 ( 3-4 ) page: 205-214   2000

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  330. Lipid raft on gametic cells as a functional domain for sperm-egg interaction coupled with signal transduction

    Ohta K, Sato C, Matsuda T, Toriyama M, Vacquier VD, Hirohashi N, Lennarz WJ, Kitajima K

    ZYGOTE   Vol. 8   page: S63-S63   2000

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  331. Lipid raft on gametic cells as a functional domain for sperm-egg interaction coupled with signal transduction Reviewed

    K Ohta, C Sato, T Matsuda, M Toriyama, VD Vacquier, N Hirohashi, WJ Lennarz, K Kitajima

    ZYGOTE   Vol. 8   page: S63 - S63   2000

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    Web of Science

  332. Glycobiology of di- and oligosialyl glycotopes

    Sato C, Kitajima K

    TRENDS IN GLYCOSCIENCE AND GLYCOTECHNOLOGY   Vol. 11 ( 62 ) page: 371-390   1999.11

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  333. Cholesterol-dependent localization of NAP-22 on a neuronal membrane microdomain (Raft)

    Maekawa S, Sato C, Kitajima K, Funatsu N, Kumanogoh H, Sokawa Y

    JOURNAL OF BIOLOGICAL CHEMISTRY   Vol. 274 ( 30 ) page: 21369-21374   1999.7

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  334. Isolation and characterization of low density detergent-insoluble membrane (LD-DIM) fraction from sea urchin sperm

    Ohta K, Sato C, Matsuda T, Toriyama M, Lennarz WJ, Kitajima K

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   Vol. 258 ( 3 ) page: 616-623   1999.5

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  335. A 42-kDa glycoprotein from chicken egg-envelope, an avian homolog of the ZPC family glycoproteins in mammalian zona pellucida - Its first identification, cDNA cloning and granulosa cell-specific expression

    Takeuchi Y, Nishimura K, Aoki N, Adachi T, Sato C, Kitajima K, Matsuda T

    EUROPEAN JOURNAL OF BIOCHEMISTRY   Vol. 260 ( 3 ) page: 736-742   1999.3

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  336. Fluorescent-assisted detection of oligosialyl units in glycoconjugates

    Sato C, Inoue S, Matsuda T, Kitajima K

    ANALYTICAL BIOCHEMISTRY   Vol. 266 ( 1 ) page: 102-109   1999.1

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  337. Glycobiology of di and oligosialyl glycotopes. Invited Reviewed

    "C. Sato, and K. Kitajima"

    Trends Glycosci. Glycotechnol.   Vol. 11   page: 371-390   1999.1

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  338. Fluorescent-assisted detection of oligosialyl units in glycoconjugates. Reviewed

    "C. Sato, S. Inoue, T. Matsuda, and K. Kitajima"

    Anal. Biochem.   Vol. 267   page: 102-109   1999.1

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  339. A 42-kDa glycoprotein from chicken egg-envelope, an avian homolog of the ZPC family glycoproteins in mammalian zona pellucida. Its first identification, cDNA cloning and granulosa cell-specific expression. Reviewed

    "T. Takeuchi, K. Nishimura, N. Aoki, T. Adachi, C. Sato, K. Kitajima, and T. Matsuda"

    Eur. J. Biochem.   Vol. 273   page: 21526-21530   1999.1

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  340. Cholesterol-dependent localization of NAP-22 on a neuronal membrane microdomain (Raft). Reviewed

    "S. Maekawa, C. Sato, K. Kitajima, H. Kumanogoh, N. Funatsu, and Y. Sokawa"

    J. Biol. Chem.   Vol. 274   page: 21369-21374   1999.1

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  341. Isolation and characterization of low density detergent-insoluble membrane (LD-DIM) fraction from sea urchin sperm. Reviewed

    "K. Ohta, C. Sato, T. Matsuda, M. Toriyama, W. J. Lennarz, and K. Kitajima"

    Biochem. Biophys. Res. Commun.   Vol. 258   page: 616-623   1999.1

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  342. A 42-kDa glycoprotein from chicken egg-envelope, an avian homolog of the ZPC family glycoproteins in mammalian zona pellucida. Its first identification, cDNA cloning and granulosa cell-specific expression. Reviewed

    T. Takeuchi, K. Nishimura, N. Aoki, T. Adachi, C. Sato, K. Kitajima, T. Matsuda

    Eur. J. Biochem.   Vol. 273   page: 21526-21530   1999.1

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  343. Glycobiology of di and oligosialyl glycotopes. Invited Reviewed

    C. Sato, K. Kitajima

    Trends Glycosci. Glycotechnol.   Vol. 11   page: 371-390   1999.1

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  344. Fluorescent-assisted detection of oligosialyl units in glycoconjugates. Reviewed

    C. Sato, S. Inoue, T. Matsuda, K. Kitajima

    Anal. Biochem.   Vol. 267   page: 102-109   1999.1

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  345. Cholesterol-dependent localization of NAP-22 on a neuronal membrane microdomain (Raft). Reviewed

    S. Maekawa, C. Sato, K. Kitajima, H. Kumanogoh, N. Funatsu, Y. Sokawa

    J. Biol. Chem.   Vol. 274   page: 21369-21374   1999.1

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  346. Isolation and characterization of low density detergent-insoluble membrane (LD-DIM) fraction from sea urchin sperm. Reviewed

    K. Ohta, C. Sato, T. Matsuda, M. Toriyama, W. J. Lennarz, K. Kitajima

    Biochem. Biophys. Res. Commun.   Vol. 258   page: 616-623   1999.1

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  347. Development of a highly sensitive chemical method for detecting alpha 2 -> 8-linked oligo/polysialic acid residues in glycoproteins blotted on the membrane

    Sato C, Inoue S, Matsuda T, Kitajima K

    ANALYTICAL BIOCHEMISTRY   Vol. 261 ( 2 ) page: 191-197   1998.8

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  348. Identification of oligo-N-glycolylneuraminic acid residues in mammal-derived glycoproteins by a newly developed immunochemical reagent and biochemical methods

    Sato C, Kitajima K, Inoue S, Inoue Y

    JOURNAL OF BIOLOGICAL CHEMISTRY   Vol. 273 ( 5 ) page: 2575-2582   1998.1

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  349. Development of a highly sensitive chemical method to detect a2ョ8-linked oligo/polysialic acid residues in glycoproteins blotted on the membrane. Reviewed

    "C. Sato, S. Inoue, T. Matsuda, and K. Kitajima"

    Anal. Biochem.   Vol. 261   page: 191-197   1998.1

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  350. Identification of oligo-N-glycolylneuraminic acid residues in mammal-derived glycoproteins by a newly developed immunochemical reagent and biochemical methods. Reviewed

    "C. Sato, K. Kitajima, S. Inoue, and Y. Inoue"

    J. Biol. Chem.   Vol. 273   page: 2575-2582   1998.1

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  351. Development of a highly sensitive chemical method to detect a2ョ8-linked oligo/polysialic acid residues in glycoproteins blotted on the membrane. Reviewed

    C. Sato, S. Inoue, T. Matsuda, K. Kitajima

    Anal. Biochem.   Vol. 261   page: 191-197   1998.1

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  352. Identification of oligo-N-glycolylneuraminic acid residues in mammal-derived glycoproteins by a newly developed immunochemical reagent and biochemical methods. Reviewed

    C. Sato, K. Kitajima, S. Inoue, Y. Inoue

    J. Biol. Chem.   Vol. 273   page: 2575-2582   1998.1

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  353. Occurrence of terminal alpha 2-&gt;8-linked disialylated poly-N-acetyllactosamine chains with Le(X) and I antigenic glycotopes in tetraantennary arms of an N-linked glycoprotein isolated from rainbow trout ovarian fluid Reviewed

    Y Funakoshi, T Taguchi, C Sato, K Kitajima, S Inoue, HR Morris, A Dell, Y Inoue

    GLYCOBIOLOGY   Vol. 7 ( 2 ) page: 195 - 205   1997.3

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    The Pronase digestion of a 54K glycoprotein present in ovarian fluid of rainbow trout yielded a major glycopeptide, Carbohydrate compositional analysis revealed that this glycopeptide was likely to possess a single large N-glycan chain having low molecular weight oligomers of N-acetylneuraminic acid (oligoNeu5Ac), Structural studies of this glycopeptide revealed novel alpha 2 --&gt; 8-linked disialylated poly-N-acetyllactosamine chains with Le(X) and I antigenic determinants on the N-linked tetraantennary core glycan, In our recent studies (Kitazume,S., Kitajima,K., Inoue,S., Inoue,Y. and Troy,F.A. (1994) J., Biol, Chem, 269, 10330-10340) we presented evidence that synthesis of alpha 2 --&gt; 8-linked polysialic acid (polySia) chains is a two-step process in which chain initiation is catalyzed by an alpha 2 --&gt; 8-sialyltransferase (alpha 2 --&gt; 8-ST; initiase) that catalyzes synthesis of the first Sia alpha 2 --&gt; 8-linkage, forming the disialic acid (diSia) unit, Sia alpha 2 --&gt; 8-Sia alpha 2 --&gt; 6-Gal-.Chain polymerization is then postulated to be catalyzed by a second enzyme, an alpha 2 --&gt; 8-polyST (''polymerase'') that converts the diSia units to polySia chains, The present structural studies leading to the discovery of alpha 2 --&gt; 8-linked disialylated units that terminate poly-N-acetyllactosamine chains in an N-linked glycoprotein is further evidence in support of our hypothesis that more than one sialyltransferase activity is required for polySia chain synthesis and polymerization.

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  354. Occurrence of terminal alpha2,8-linked disialylated poly-N-acetyllactosamine chains with LeX and I antigenic glycotopes in tetraantennary arms of an N-linked glycoprotein isolated from rainbow trout ovarian fluid. Reviewed

    "Y. Funakoshi, T. Taguchi, C. Sato, K. Kitajima, S. Inoue, H. R. Morris, A. Dell, and Y. Inoue"

    Glycobiology   Vol. 7   page: 195-205   1997.1

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  355. Characterization of the antigenic specificity of four different anti-(alpha2,8-linked polysialic acid) antibodies using lipid-conjugated oligo/polysialic acids. Reviewed

    "C. Sato, K. Kitajima, S. Inoue, T. Seki, F. A. Troy, II, and Y. Inoue"

    J. Biol. Chem.   Vol. 270   page: 18923-18928   1995.1

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  356. Characterization of the antigenic specificity of four different anti-(alpha2,8-linked polysialic acid) antibodies using lipid-conjugated oligo/polysialic acids. Reviewed

    C. Sato, K. Kitajima, S. Inoue, T. Seki, F. A. Troy, II, Y. Inoue

    J. Biol. Chem.   Vol. 270   page: 18923-18928   1995.1

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  357. Structural diversity in a2,8-linked polysialic acid chains in salmonid fish egg glycoproteins. Reviewed

    C. Sato, K. Kitajima, I. Tazawa, Y. Inoue, S. Inoue, and F. A. Troy, II

    J. Biol. Chem.   Vol. 268   page: 23675-23684   1993

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  358. Structural diversity in a2,8-linked polysialic acid chains in salmonid fish egg glycoproteins. Reviewed

    C. Sato, K. Kitajima, I. Tazawa, Y. Inoue, S. Inoue, F. A. Troy, II

    J. Biol. Chem.   Vol. 268   page: 23675-23684   1993

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Books 43

  1. Role of Polysialic Acid in Schizophrenia

    Sato C., Hane M., Kitajima K.

    Comprehensive Glycoscience: Second Edition  2021.6  ( ISBN:9780128222447

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  2. Polysialic acid as an integrative decoder in nervous and reproductive systems

    Sato C., Kitajima K.

    Glycome: The Hidden Code in Biology  2021.4  ( ISBN:9781536194371

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    Language:Japanese

    In gametes, embryos, and neural cells, huge glycans with >10 kDa are often attached on glycoconjugates. Of those, the polysialic acid (polySia) modifies the neural cell adhesion molecules and other particular proteins, playing multiple roles in regulation of cell surface phenomena. Underlying mechanisms for the polySia-related functions are twofold. One is due to structural diversity of polySia arisen from differences in the Sia species, the inter-residue linkage, and the degree of polymerization (DP). Significance of the DP has been clarified, and not only lectins but also physiologically active molecules recognize the polymerized structures of Sia in a DP-dependent manner to trigger the different downstream signals. The other is based on the repulsive and attractive interactions of polySia with polySia itself and other binding counterpart molecules including BDNF and FGF2. Here we show that, as a code for cell surface event, the polymerized structures of Sia are expressed and interacted with various decoders, such as Siglecs, BDNF, FGF2, and dopamine, which specifically bind each of their diverse structures, diSia, triSia, oligoSia and polySia. Especially, polySia is shown to display repulsive and attractive fields at the same time to implement integrated functions of various related physiologically active molecules.

    Scopus

  3. 糖鎖生物学 : 生命現象と糖鎖情報

    北島 健, 佐藤 ちひろ, 門松 健治, 加藤 晃一( Role: Joint author)

    名古屋大学出版会  2020  ( ISBN:9784815809812

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    Language:Japanese Book type:Scholarly book

    CiNii Books

  4. ST8 alpha-N-acetyl-neuraminide alpha- 2,8-sialyltransferase 2 (ST8SIA2)

    Sato C.

    Handbook of Glycosyltransferases and Related Genes, Second Edition  2014.1  ( ISBN:9784431542391

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    Language:Japanese

    ST8 α-N-acetyl-neuraminide α-2,8-sialyltransferase 2 (ST8SIA2) is a member of a family of α2,8-sialyltransferases that transfer sialic acid from CMP-sialic acid onto sialic acid residues of other sialoglycans to generate α2,8-linked sialoglycans. ST8SIA2 is characterized by the ability to synthesize polysialic acid (polySia) structure and can therefore be subclassified as a polysialyltransferase. Both ST8SIA2 and the related polysialyltransferase ST8SIA4 (refer to the Chap. ST8SIA4) are capable of synthesizing extraordinary long polySia chains with degrees of polymerization (DP) of up to 400. ST8SIA2 is a type II transmembrane protein, similar to many other glycosyltransferases, and is composed of 375 amino acids with six N-glycans. This enzyme contains sialyl-motif (SM) L, S, VS, and III domains.

    DOI: 10.1007/978-4-431-54240-7_77

    Scopus

  5. Polysialic acid

    Sato C.

    Sialobiology: Structure, Biosynthesis and Function. Sialic Acid Glycoconjugates in Health and Disease  2013.8  ( ISBN:9781608050673

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    Language:Japanese

    Sialic acids (Sia) are involved in many biological activities and are commonly present as monosialyl residues at the non-reducing terminal end of glycoconjugates. Occasionally, polymerized structures in the form of disialic acid (diSia), oligosialic acid (oligoSia), and polysialic acid (polySia) appear in glycoconjugates. In particular, polySia is known to be a common epitope from bacteria to humans and is one of the most famous, biologically-important glycotopes in vertebrates. The biological functions of polySia, especially on neural cell adhesion molecules (NCAMs), have been well studied and an indepth body of knowledge concerning polySia has been accumulated. However, considerably less attention has been paid to glycoproteins containing di- and oligoSia groups. As the analytical methods used to detect oligo/polymerized structures have been improved, glycoproteins containing di/oligo/polySia chains have been identified with an increasing frequency in nature. In addition, more sophisticated genetic techniques have helped elucidate the underlying mechanisms of polySia-mediated activities. In this chapter, the recent advances in the study of di-, oligo- and polySia residues on glycoproteins, including their distribution, chemical properties, biosynthetic pathways, and functions are described. © 2013 Bentham Science Publishers. All rights reserved.

    DOI: 10.2174/9781608053865113010005

    Scopus

  6. Advanced Technologies in Sialic Acid and Sialoglycoconjugate Analysis in Top Curr Chem

    Kitajima K, Varki N, Sato C.( Role: Joint author)

    Springer  2015 

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    Language:English

  7. Advanced Technologies in Sialic Acid and Sialoglycoconjugate Analysis in Top Curr Chem

    Kitajima K, Varki N, Sato C( Role: Joint author)

    Springer  2015 

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    Responsible for pages:75-103   Language:English

  8. Frontal Affinity Chromatography in the Methods in Molecular Biology

    Sato, C. ( Role: Sole author)

    Springer  2014 

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    Language:English

  9. Flagellasialin in the Glycoscience: Biology and Medicine

    Kanazawa, T., Suzuki, E., Miyata, S., Sato, C. and Kitajima, K. ( Role: Joint author)

    Springer  2014 

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    Language:English

  10. Polysialyltransferase assay in the Glycoscience: Biology and Medicine

    Hane, M., Kitajima, K. and Sato, C. ( Role: Joint author)

    Springer  2014 

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    Language:English

  11. Polysialic acid in the Glycoscience: Biology and Medicine

    Sato, C. and Kitajima, K. ( Role: Joint author)

    Springer,  2014 

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    Language:English

  12. ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 2 (ST8SIA2)in Handbook of Glycosyltransferases and related genes

    Sato C( Role: Sole author)

    Springer  2014 

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    Language:English

  13. ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 2 (ST8SIA2)in Handbook of Glycosyltransferases and related genes

    Sato C( Role: Sole author)

    Springer  2014 

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    Responsible for pages:781-795   Language:English

  14. Flagellasialin in the Glycoscience: Biology and Medicine

    Kanazawa, T, Suzuki, E, Miyata, S, Sato, C, Kitajima, K( Role: Joint author)

    Springer  2014 

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    Responsible for pages:1-6   Language:English

  15. Polysialyltransferase assay in the Glycoscience: Biology and Medicine

    Hane, M, Kitajima, K, Sato, C( Role: Joint author)

    Springer  2014 

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    Responsible for pages:1-5   Language:English

  16. Polysialic acid in the Glycoscience: Biology and Medicine

    Sato, C, Kitajima, K( Role: Joint author)

    Springer,  2014 

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    Responsible for pages:1-7   Language:English

  17. Frontal Affinity Chromatography in the Methods in Molecular Biology

    Sato, C( Role: Sole author)

    Springer  2014 

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    Responsible for pages:257-264   Language:English

  18. Sialobiology: Structure, Function and Biosynthesis

    Sato C( Role: Joint author)

    Bentham Science  2013 

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    Language:English

  19. Sialobiology: Structure, Function and Biosynthesis

    Sato C( Role: Joint author)

    Bentham Science  2013 

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    Responsible for pages:33-75   Language:English

  20. Methods in Enzymology・ Analysis of glycan-protein interaction by frontal affinity chromatography and Biacore.

    Sato, C., Yamakawa, N., and Kitajima, K.( Role: Joint author)

    Elsevir science  2010 

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    Language:English

  21. Methods in Enzymology・ Analysis of glycan-protein interaction by frontal affinity chromatography and Biacore.

    Sato, C, Yamakawa, N, Kitajima, K( Role: Joint author)

    Elsevir science  2010 

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    Responsible for pages:219-232   Language:English

  22. Carbohydrate Recognition: Biological problems, methods, and applications

    Ken Kitajima and Chihiro Sato (Editors: Binghe Wang, Geer-Jan Boons)( Role: Joint author)

    John Wiley and Sons  2009 

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    Language:English

  23. Carbohydrate Recognition: Biological problems, methods, and applications

    Ken Kitajima, Chihiro Sato, Binghe Wang, Geer-Jan Boons( Role: Joint author)

    John Wiley and Sons  2009 

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    Responsible for pages:in press   Language:English

  24. Membrane microdomain as a platform of carbohydrate-mediated interactions during early development of medaka fish.

    Adachi, T., Sato, C., and Kitajima, K.( Role: Joint author)

    KINPO-DO  2008 

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    Language:English

  25. Glycoscience Lab Manual・Structure analysis of oligo/polysialic acid

    C. Sato and K. Kitajima( Role: Joint author)

    KINPO-DO  2008 

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    Language:English

  26. Glycoscience Lab Manual・Labeling of oligosialic acid

    C. Sato( Role: Joint author)

    KINPO-DO  2008 

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    Language:English

  27. Glycoscience Lab Manual・Labeling of oligosialic acid

    C. Sato( Role: Joint author)

    KINPO-DO  2008 

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    Responsible for pages:24-27   Language:English

  28. Membrane microdomain as a platform of carbohydrate-mediated interactions during early development of medaka fish.

    Adachi, T, Sato, C, Kitajima, K( Role: Joint author)

    KINPO-DO  2008 

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    Responsible for pages:299-302   Language:English

  29. Glycoscience Lab Manual・Structure analysis of oligo/polysialic acid

    C. Sato, K. Kitajima( Role: Joint author)

    KINPO-DO  2008 

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    Responsible for pages:77-81   Language:English

  30. 遺伝子医学MOOK 糖鎖と病気・ポリシアル酸

    佐藤ちひろ、北島 健( Role: Joint author)

    メディカルデュー  2005 

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    Language:Japanese

  31. 未来を拓く糖鎖科学・オリゴシアル酸の標識

    ( Role: Joint author)

    金芳堂  2005 

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    オリゴシアル酸の標識

  32. 未来を拓く糖鎖科学・オリゴシアル酸の標識

    ( Role: Joint author)

    金芳堂  2005 

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    Responsible for pages:12-13   Language:Japanese

    オリゴシアル酸の標識

  33. 遺伝子医学MOOK 糖鎖と病気・ポリシアル酸

    佐藤ちひろ, 北島 健( Role: Joint author)

    メディカルデュー  2005 

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    Responsible for pages:186-191   Language:Japanese

  34. Recent Research Developments in Analytical Biochemistry・Detection of di/oligo/polysialic acid units in glycoproteins

    "C. Sato, and K. Kitajima"( Role: Joint author)

    Transworld research network  2002 

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    Language:English

  35. Recent Research Developments in Analytical Biochemistry・Detection of di/oligo/polysialic acid units in glycoproteins

    C. Sato, K. Kitajima( Role: Joint author)

    Transworld research network  2002 

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    Responsible for pages:201-215   Language:English

  36. Leukocyte typing VII・Carbohydrate-recognizing monoclonal antibodies as revealed by ELISA using phosphatidylethanolamine-conjugated oligosaccharides as antigens

    C. Sato and K. Kitajima( Role: Joint author)

    OXFORD  2001 

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  37. Leukocyte typing VII・Oligosialic acid as a differentiation-associated antigen in human haematopoietic cell line HL60.

    C. Sato and K. Kitajima( Role: Joint author)

    OXFORD  2001 

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  38. Leukocyte typing VII・Carbohydrate-recognizing monoclonal antibodies as revealed by ELISA using phosphatidylethanolamine-conjugated oligosaccharides as antigens

    C. Sato, K. Kitajima( Role: Joint author)

    OXFORD  2001 

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    Responsible for pages:168-169   Language:English

  39. Leukocyte typing VII・Oligosialic acid as a differentiation-associated antigen in human haematopoietic cell line HL60.

    C. Sato, K. Kitajima( Role: Joint author)

    OXFORD  2001 

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    Responsible for pages:208-211   Language:English

  40. Sialobiology and Other Novel Form of Glycosylation・Occurrence of a2,8-linked oligosialic acid residues in mammalian glycoproteins.

    K. Kitajima, C. Sato, N. Honda, T. Matsuda, M.-H. Yokoyama, B. E. Close, and K. J. Colley( Role: Joint author)

    Gakushin  1999 

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  41. Sialobiology and Other Novel Form of Glycosylation・Occurrence of a2,8-linked oligosialic acid residues in mammalian glycoproteins.

    K. Kitajima, C. Sato, N. Honda, T. Matsuda, M.-H. Yokoyama, B. E. Close, K. J. Colley( Role: Joint author)

    Gakushin  1999 

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    Responsible for pages:69-76   Language:English

  42. KDN(=デアミノノイラミン酸)-転移酸素およびポリKDN-転移酵素の研究 : 各種KDN-転移酵素の利用による機能性KDN-複合糖質の造成と活用

    井上 康男( Role: Sole author)

    井上康男  1994 

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    Language:Japanese

    CiNii Books

  43. KDN(=デアミノノイラミン酸)-転移酸素およびポリKDN-転移酵素の研究 : 各種KDN-転移酵素の利用による機能性KDN-複合糖質の造成と活用

    井上 康男( Role: Sole author)

    井上康男  1994 

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    Responsible for pages:.   Language:Japanese

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MISC 24

  1. 胎仔期マウス大脳皮質形成におけるヒアルロン酸の発現変動と細胞外基質としての機能

    武智美奈, 大島健司, 灘野大太, 松田幹, 佐藤ちひろ, 北島健, 北川裕之, 宮田真路, 宮田真路

    日本生化学会大会(Web)   Vol. 92nd   2019

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  2. Binding Activity of Hsp70 toward Acidic Glycoconjugates : An Unexpected Role of Sugars

      Vol. 55 ( 1 ) page: 22 - 26   2017.1

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    Language:Japanese  

    CiNii Books

  3. The Out of Africa and mental disorders: Adaptation with reduction in STX promoter activity

    Naoko Fujito, Yoko Satta, Masaya Hane, Atsushi Matsui, Kenta Yashima, Ken Kitajima, Chihiro Sato, Naoyuki Takahata, Toshiyuki Hayakawa

    GENES & GENETIC SYSTEMS   Vol. 91 ( 6 ) page: 343 - 343   2016.12

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    Language:English   Publishing type:Research paper, summary (international conference)   Publisher:GENETICS SOC JAPAN  

    Web of Science

  4. A Uniquely Human Evolutionary Change in ST8Sia-II Impacts Enzyme Stability and Polysialic Acid Function

    Michael Vaill, Masaya Hane, Yuko Naito-Matsui, Sandra Diaz, Leela Davies, Ken Kitajima, Chihiro Sato, Ajit Varki

    GLYCOBIOLOGY   Vol. 26 ( 12 ) page: 1423 - 1423   2016.12

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    Language:English   Publishing type:Research paper, summary (international conference)   Publisher:OXFORD UNIV PRESS INC  

    Web of Science

  5. Spread of reduced activity of STX promoter throughout Great Journey

    Naoko Fujito, Yoko Satta, Masaya Hane, Atsushi Matsui, Ken Kitajima, Chihiro Sato, Toshiyuki Hayakawa

    GENES & GENETIC SYSTEMS   Vol. 90 ( 6 ) page: 379 - 379   2015.12

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    Web of Science

  6. Adaptive evolution of the promoter region of the Sialyltransferase 8B (STX) gene

    Naoko T. Fujito, Toshiyuki Hayakawa, Masaya Hane, Ken Kitajima, Chihiro Sato, Yoko Satta

    GENES & GENETIC SYSTEMS   Vol. 89 ( 6 ) page: 327 - 327   2014.12

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    Web of Science

  7. The Synthesis of Sialo-containing Glycopolymers by pi-Allyl Nickel Catalyzed Coordination Polymerization

    Shuichi Ohira, Yu Yasuda, Chihiro Sato, Ken KItajima, Ikuyoshi Tomita, Takashi Takahashi, Hiroshi Tanaka

    GLYCOBIOLOGY   Vol. 24 ( 11 ) page: 1119 - 1120   2014.11

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    Web of Science

  8. Insight into single nucleotide polymorphisms (SNPs) of the polysialyltransferase ST8SIA2/STX in psychiatric disorders

    Masaya Hane, Saki Nishimura, Toshiyuki Hayakawa, Ken Kitajima, Chihiro Sato

    GLYCOBIOLOGY   Vol. 24 ( 11 ) page: 1206 - 1206   2014.11

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    Web of Science

  9. An anti-schizophrenic drug affects the surface expression of polySia-NCAM in IMR-32 human neuroblastoma cells

    Saki Nishimura, Masaya Hane, Yuki Niimi, Ken Kitajima, Chihiro Sato

    GLYCOBIOLOGY   Vol. 24 ( 11 ) page: 1193 - 1193   2014.11

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    Language:English   Publishing type:Research paper, summary (international conference)   Publisher:OXFORD UNIV PRESS INC  

    Web of Science

  10. Functional analysis of tissue-specific post-translational modification of springtime hormone TSH

    IKEGAMI KEISUKE, LIAO XIAO-HUI, HOSHINO YUTA, ONO HIROKO, OTA WATARU, ITO YUKA, NISHIWAKI-OHKAWA TAEKO, SATO CHIHIRO, KITAJIMA KEN, IIGO MASAYUKI, SHIGEYOSHI YASUFUMI, YAMADA MASANOBU, MURATA YOSHIHARU, REFETOFF SAMUEL, YOSHIMURA TAKASHI

    時間生物学   Vol. 20 ( 2 ) page: 114   2014.10

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    J-GLOBAL

  11. ポリシアル酸転移酵素STX/ST8SIA2とPST/ST8SIA4のポリシアル酸生合成能の解析

    新美百希, 羽根正弥, 西村紗希, 宮田真路, 北島健, 佐藤ちひろ

    日本糖質学会年会要旨集   Vol. 33rd   page: 144   2014.7

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    J-GLOBAL

  12. 癌や精神疾患に関わるポリシアル酸構造に特異的な一本鎖抗体の作製と特異性評価

    羽根正弥, 羽根正弥, 羽根正弥, 長江雅倫, 池田明美, 山口芳樹, 北島健, 北島健, 北島健, 佐藤ちひろ, 佐藤ちひろ, 佐藤ちひろ

    日本農芸化学会大会講演要旨集(Web)   Vol. 2014   2014

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  13. Polysialic acid on flagellasialin is involved in sperm motility

    Shinji Miyata, Chihiro Sato, Hironobu Kumita, Masaru Toriyama, Ken Kitajima

    ZOOLOGICAL SCIENCE   Vol. 23 ( 12 ) page: 1171 - 1171   2006.12

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    Web of Science

  14. An N-terminal 130 kDa fragment of a fourth sea urchin sperm receptor for egg jelly module protein, REJ4, is secreted during acrosome reaction

    Shinji Miyata, Chihiro Sato, Masaru Toriyama, Ken Kitajama

    ZOOLOGICAL SCIENCE   Vol. 22 ( 12 ) page: 1456 - 1456   2005.12

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    Web of Science

  15. Capacitation-induced changes of the surface distribution of sialoglycotopes on pig sperm

    Lian Xue, Shinji Miyata, Chihiro Sato, Ken Kitajima

    ZOOLOGICAL SCIENCE   Vol. 22 ( 12 ) page: 1456 - 1456   2005.12

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    Web of Science

  16. ブタ精子表面に新しく見出されたシアロ糖鎖エビトープの解析

    せつ蓮, 宮田真路, 佐藤ちひろ, 北島健

    生化学   Vol. 77 ( 6 ) page: 571   2005.6

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    J-GLOBAL

  17. The 130 kDa glycoprotein is secreted from sperm during acrosome reaction of sea urchin, Hemicentrotus pulcherrimus

    Shinji Miyata, Chihiro Sato, Masaru Toriyama, Noritaka Hirohashi, Victor Vacquier, Ken Kitajima

    ZOOLOGICAL SCIENCE   Vol. 21 ( 12 ) page: 1292 - 1292   2004.12

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    Web of Science

  18. A major ganglioside from Hemicentrotus pulcherrimus sperm specifically interacts with phospholipids

    Nao Yamakawa, Chihiro Sato, Ken Kitajima

    ZOOLOGICAL SCIENCE   Vol. 21 ( 12 ) page: 1292 - 1292   2004.12

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    Web of Science

  19. Development of sensitive chemical and immunochemical methods for detecting sulfated sialic acids and their application to localization and quantitation studies of sea urchin sperm and eggs

    N Yamakawa, E Maehashi, S Miyata, C Sato, K Furuhata, K Kitajima

    GLYCOBIOLOGY   Vol. 14 ( 11 ) page: 1146 - 1146   2004.11

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    Language:English   Publishing type:Research paper, summary (international conference)   Publisher:OXFORD UNIV PRESS INC  

    Web of Science

  20. Occurrence and characterization of a novel sulfated alpha 2,9-linked polysialic acid-containing glycoprotein in sea urchin sperm flagellum

    S Miyata, C Sato, K Kitajima

    GLYCOBIOLOGY   Vol. 14 ( 11 ) page: 1146 - 1146   2004.11

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    Web of Science

  21. Mouse T cell CD166 is a disialic acid-containing glycoprotein and involved in T cell activation

    C Sato, K Nohara, T Matsuda, K Kitajima

    GLYCOBIOLOGY   Vol. 14 ( 11 ) page: 1136 - 1136   2004.11

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    Web of Science

  22. Mouse heat shock protein 70 recognizes negatively charged glycolipids and phospholipids

    Y Harada, C Sato, K Kitajima

    GLYCOBIOLOGY   Vol. 14 ( 11 ) page: 1101 - 1102   2004.11

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    Web of Science

  23. Effects of exogenously administered mannose and deaminoneuraminic acid (KDN) to mice or murine cultured cells on the metabolism of KDN

    S Go, C Sato, K Kitajima

    GLYCOBIOLOGY   Vol. 14 ( 11 ) page: 1166 - 1167   2004.11

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    Web of Science

  24. ウニ受精におけるシアル酸を介する相互作用と脂質ラフトの重要性

    前橋絵梨, 宮田真路, 松本晴美, 松田幹, 佐藤ちひろ, 北島健

    生化学   Vol. 74 ( 8 ) page: 777   2002.8

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    J-GLOBAL

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Presentations 168

  1. 遺伝的及び環境要因による糖鎖発現制御から脳の健康を考える Invited

    佐藤ちひろ

    第11回グライコバイオロジクス研究会   2021.1.21  第11回グライコバイオロジクス研究会

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    Event date: 2021.1

    Language:Japanese   Presentation type:Oral presentation (keynote)  

    Venue:名古屋   Country:Japan  

  2. オリゴシアル酸転移酵素ST8Sia6の発現はメラノーマを悪性化する

    畑中理菜、荒木映莉乃、羽根正弥、Wu Di、北島健、佐藤ちひろ

    第93回日本生化学会大会  2020.9.14  日本生化学会

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    Event date: 2020.9

    Language:English   Presentation type:Oral presentation (general)  

    Venue:Web   Country:Japan  

  3. 環境要因としてのシアル酸投与と脳領域特異的ポリシアル酸発現の解析

    高橋佑佳、阿部智佳羅、羽根正弥、呉迪、北島 健、佐藤ちひろ

    第84回日本生化学会中部支部例会  2020.9.14  日本生化学会中部支部

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    Event date: 2020.9

    Language:Japanese   Presentation type:Poster presentation  

    Venue:Web   Country:Japan  

  4. 各種脊椎動物ポリシアル酸転移酵素ST8Sia2がもたらす種特異的なpolySia-NCAM構造の解析

    村井良、羽根正弥、呉迪、北島健、佐藤ちひろ

    第93回日本生化学会大会  2020.9.14  日本生化学会

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    Event date: 2020.9

    Language:Japanese   Presentation type:Poster presentation  

    Venue:Web   Country:Japan  

  5. ラクタム化シアル酸誘導体の合成とその分解反応に関する研究.

    大野ひろみ、安藤弘宗、川上信彦、岩田章子、佐藤ちひろ、北島健、石田秀治、木曽真

    日本農芸化学会2009年度大会 

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    Event date: 2009.3

    Language:Japanese   Presentation type:Poster presentation  

    Country:Japan  

  6. 魚類CMP-シアル酸合成酵素の構造と性質

    藤田明子、安川裕子、佐藤ちひろ、北島健

    日本農芸化学会2009年度大会 

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    Event date: 2009.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  7. ポリシアル酸と脳由来神経栄養因子の相互作用の解析

    金戸幸弘、北島健、佐藤ちひろ

    日本農芸化学会2009年度大会 

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    Event date: 2009.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  8. ポリシアル酸とFGF-2の相互作用の解析.

    小野明日香、金戸幸弘、北島 健、佐藤ちひろ

    日本農芸化学会2009年度大会 

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    Event date: 2009.3

    Language:Japanese   Presentation type:Poster presentation  

    Country:Japan  

  9. Polysialic acid is not a negative regulator any longer in neurogenesis and neuronal functions: A new finding of its binding to a particular group of neurotransmitters. International conference

    Ken Kitajima, Ryo Isomura, and Chihiro Sato

    Gordon Research Conference on Glycobiology,  

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    Event date: 2009.1

    Language:English   Presentation type:Poster presentation  

    Country:United States  

  10. 昆虫細胞由来シアル酸9-リン酸合成酵素の構造と基質特異性の解析

    濱口香代、郷慎司、佐藤ちひろ、小林迪弘、池田素子、北島健

    第81回日本生化学会大会 

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    Event date: 2008.12

    Language:Japanese   Presentation type:Poster presentation  

    Country:Japan  

  11. 抗ジシアル酸抗体が認識する急性期タンパク質の同定. International conference

    安川然太、北島健、佐藤ちひろ

    第81回日本生化学会大会 

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    Event date: 2008.12

    Language:English   Presentation type:Poster presentation  

    Country:Japan  

  12. 化学的検出法を用いた天然におけるサイクリックシアル酸の局在検索.

    岩田章子、佐藤ちひろ、安藤弘宗、石田秀治、木曽真、神奈木玲児、北島健

    第81回日本生化学会大会 

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    Event date: 2008.12

    Language:Japanese   Presentation type:Poster presentation  

    Country:Japan  

  13. ポリシアル酸は特定の神経伝達物質と相互作用する. International conference

    磯村遼、北島健、佐藤ちひろ

    第81回日本生化学会大会 

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    Event date: 2008.12

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  14. mAb.A2B5の抗原特異性の決定とマウス脳発達段階におけるmAb.A2B5反応性オリゴシアル酸含有糖タンパク質の発現解析 International conference

    猪子恵未、西浦祐二、田中浩士、高橋孝志、北島健、佐藤ちひろ

    第81回日本生化学会大会 

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    Event date: 2008.12

    Language:English   Presentation type:Poster presentation  

    Country:Japan  

  15. 酸性多糖と神経栄養因子との相互作用解析. International conference

    金戸幸弘、北島健、佐藤ちひろ

    第81回日本生化学会大会 

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    Event date: 2008.12

    Language:English   Presentation type:Oral presentation (general)  

    Country:Japan  

  16. 哺乳類シアロ糖タンパク質のデアミノノイラミン酸 (KDN) 修飾糖タンパク質への改変.

    中島優子、藤田明子、佐藤ちひろ、北島健

    第81回日本生化学会大会 

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    Event date: 2008.12

    Language:Japanese   Presentation type:Poster presentation  

    Country:Japan  

  17. ポリシアル酸の新機能

    佐藤ちひろ

    第6回日本糖鎖科学コンソーシアムシンポジウム-糖鎖研究と他領域との統合- 

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    Event date: 2008.12

    Language:Japanese   Presentation type:Symposium, workshop panel (nominated)  

    Country:Japan  

  18. ウニ配偶子生体膜マイクロドメイン間接着に関わるガングリオシド-中性糖脂質相互作用の発見.

    山川奈緒、佐藤ちひろ、北島健

    第81回日本生化学会大会 

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    Event date: 2008.12

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  19. Novel insights into the novel binding sites of the sialic acid biding Ig-like lectin-7 (Siglec-7) gained by in silico docking studies. International conference

    A one-day symposium on Sialyltransferases, Functional Glycomics, and Genomics of Zebrafish, 

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    Event date: 2008.11

    Language:English   Presentation type:Symposium, workshop panel (nominated)  

    Country:Taiwan, Province of China  

  20. Membrane microdomains from early gastrula embryos of medaka are a platform of E-cadherin- and LeX-mediated cell-cell interactions during epiboly. 14th

    Japanese Medaka and Zebrafish Meeting 

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    Event date: 2008.9

    Language:English   Presentation type:Oral presentation (general)  

    Country:Japan  

  21. CMP-[14C]デアミノノイラミン酸(KDN)のワンポット酵素合成法の確立

    中島優子、藤田明子、佐藤ちひろ、北島健

    糖鎖科学名古屋拠点「若手の力」フォーラム第6回 

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    Event date: 2008.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  22. 酸性多糖と神経栄養因子との相互作用解析

    金戸幸広、北島 健、佐藤ちひろ

    糖鎖科学名古屋拠点「若手の力」フォーラム第6回 

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    Event date: 2008.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  23. 鱗翅目昆虫細胞におけるシアル酸9-リン酸合成酵素ホモログの同定.

    濱口香代、郷慎司、佐藤ちひろ、小林迪弘、池田素子、北島健

    糖鎖科学名古屋拠点「若手の力」フォーラム第6回 

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    Event date: 2008.8

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  24. CMP-シアル酸合成酵素の基質認識および細胞内局在の分子機構の解明

    藤田明子, 佐藤ちひろ, Tiralongo, Joe, Münster-Kühnel, Anja K., Gerardy-Schahn, Rita, 北島健

    第28回日本糖質学会年会 

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    Event date: 2008.8

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  25. 抗ガングリオシド抗体A2B5が認識する糖タンパク質のマウス新生児脳における発現.

    猪子恵未、西浦祐二、田中浩士、高橋孝志、北島健、佐藤ちひろ

    FCCAグライコサイエンス若手フォーラム2008 

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    Event date: 2008.8

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  26. ウニ卵膜マイクロドメインに対する単クローン抗体の認識エピトープ解析.

    中村奈央子、前橋絵梨、山川奈緒、佐藤ちひろ、鳥山優、北島健

     FCCAグライコサイエンス若手フォーラム2008 

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    Event date: 2008.8

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  27. ポリシアル酸の神経伝達分子結合活性の発見と性質

    磯村遼、北島健、佐藤ちひろ

    FCCAグライコサイエンス若手フォーラム2008 

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    Event date: 2008.8

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  28. Complex formation between polysialic acid and brain derived neurotrophic factor (BDNF). International conference

    Chihiro Sato, Yukihiro Kanato, and Ken Kitajima:

    24th International Carbohydrate Symposium, 

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    Event date: 2008.7 - 2008.8

    Language:English   Presentation type:Poster presentation  

  29. In vitro and in silico docking studies of heat shock protein (Hsp) 70 with sulfatide. International conference

    Nao Yamakawa, Yoichiro Harada, Chihiro Sato, Gerard Vergoten, Ken Kitajima

    24th International Carbohydrate Symposium, 

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    Event date: 2008.7 - 2008.8

    Language:English   Presentation type:Poster presentation  

  30. Brain-derived neurotrophic factor binds to polysialic acid directly depending on degree of polymerization. International conference

    Yukihiro Kanato, Ken Kitajima, Chihiro Sato

    SialoGlyco Conference 2008 

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    Event date: 2008.7

    Language:English   Presentation type:Oral presentation (invited, special)  

  31. New Findings on the Expression of Non-human Sialic Acid Neu5Gc, and KDN in Cultured Human Cells. International conference

    SialoGlyco Conference 2008 

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    Event date: 2008.7

    Language:English   Presentation type:Oral presentation (invited, special)  

  32. Occurrence of the Cyclic Sialic Acid in Mammalian Cells and Tissues As Demonstrated by Chemical Methods. International conference

    K. Kitajima, S.Iwata, N. Kawakami, C. Sato, H. Ando, H. Ohno, H. Ishida, M. Kiso, O. Taguchi, R. Kannagi:

    SialoGlyco Conference 2008 

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    Event date: 2008.7

    Language:English   Presentation type:Oral presentation (invited, special)  

  33. One-pot Enzymatic Synthesis of CMP-[14C]deaminoneuraminic Acid (KDN). International conference

    Yuko Nakajima, Akiko Fujita, Chihiro Sato, and Ken Kitajima

    SialoGlyco Conference 2008 

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    Event date: 2008.7

    Language:English   Presentation type:Poster presentation  

  34. In silico binding studies predict novel sialic acid binding sites on the sialic acid binding Ig-like lectin-7 (Siglec-7) International conference

    Nao Yamakawa, Chihiro Sato, Paul R. Crocker, Gérard Vergoten, and Ken Kitajima: I

    SialoGlyco Conference 2008 

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    Event date: 2008.7

    Language:English   Presentation type:Poster presentation  

  35. Identification of the nuclear export signals that regulate the intracellular localization of the mouse CMP-sialic acid synthetase. International conference

    Akiko Fujita, Chihiro Sato, and Ken Kitajima

    SialoGlyco Conference 2008 

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    Event date: 2008.7

    Language:English   Presentation type:Oral presentation (invited, special)  

  36. CMP-デアミノノイラミン酸(CMP-KDN)のワンポット酵素合成法の確立

    日本農芸化学会2008年度大会 

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    Event date: 2008.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  37. 抗ジシアル酸が認識するマウス血清タンパク質の同定

    安川然太、北島健、佐藤ちひろ

    日本農芸化学会2008年度大会 

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    Event date: 2008.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  38. 分子モデリング法による熱ショック蛋白質の酸性糖脂質結合性の解析

    山川奈緒、原田陽一郎、佐藤ちひろ、Gerard Vergoten、北島健

    日本農芸化学会2008年度大会 

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    Event date: 2008.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  39. マウスCMP-シアル酸合成酵素の核外移行シグナル (NES) の同定

    藤田明子、佐藤ちひろ、北島 健

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    Event date: 2008.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  40. 脊椎動物CMP-シアル酸合成酵素の基質認識および細胞内局在の分子機構の解明.

    藤田明子, 佐藤ちひろ, Tiralongo, Joe, Münster-Kühnel, Anja K., Gerardy-Schahn, Rita, 北島健

    第28回日本糖質学会年会 

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    Event date: 2007.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  41. 分子シミュレーションによる熱ショックタンパク質の酸性糖脂質結合性の解析

    山川 奈緒、原田陽一郎、佐藤ちひろ、Gerard Vergoten, 北島 健

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    Event date: 2007

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  42. Short-term regulation of polysialic acid expression in microglia

    Gordon Research Conference on Glycobiology 

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    Event date: 2007

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

  43. A rapid clearance of polysialic acid on microglia by inflammatory stimulation International conference

    3rd International Meeting on polysialic acid 

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    Event date: 2007

    Language:English   Presentation type:Poster presentation  

  44. シアル酸重合体の生物学的意義の解明

    佐藤ちひろ、矢部宇一郎、安川然太1,2、宮田真路、澤田 誠、北島 健

    文科省特定領域研究 糖鎖によるタンパク質と分子複合体の機能調節 第5回公開シンポジウム 

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    Event date: 2007

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  45. ジシアリル化修飾をうけた血清糖タンパク質の同定

    安川然太、佐野琴音、小川温子、北島 健、佐藤ちひろ

    日本農芸化学会2007年度大会 

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    Event date: 2007

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  46. 脊椎動物由来CMP-シアル酸合成酵素によるデアミノノイラミン酸(KDN)の認識機構

    藤田 明子、佐藤ちひろ、北島 健

    日本農芸化学会2007年度大会 

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    Event date: 2007

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  47. サイクリックシアル酸グリコシドの化学的性質の解析

    岩田章子、川上信彦、佐藤ちひろ、安藤弘宗、木曽 真、神奈木玲児、北島 健

    日本農芸化学会2007年度大会 

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    Event date: 2007

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  48. 酸性糖脂質による70kDa熱ショックタンパク質の複合体形成

    原田陽一郎、佐藤ちひろ、北島 健

    日本農芸化学会2007年度大会 

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    Event date: 2007

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  49. 70kDa-熱ショックタンパク質の酸性糖脂質誘導性の高分子複合体形成能の発見

    原田陽一郎、佐藤ちひろ、北島 健

    第27回 日本糖質学会年会 

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    Event date: 2007

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  50. サイクリックシアル酸グリコシドの酸加水分解産物の同定

    川上信彦、岩田章子、佐藤ちひろ、安藤弘宗、木曽 真、神奈木玲児、北島健

    糖鎖科学名古屋拠点 若手の力フォーラム 第5回 

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    Event date: 2007

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  51. O-型糖鎖上のポリシアル酸の生合成機構の解明

    朝比奈慎二、佐藤ちひろ、松野みどり、松田 幹、Karen, J. Colley, 北島 健

    糖鎖科学名古屋拠点 若手の力フォーラム 第4回 

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    Event date: 2006

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  52. ポリシアル酸の探求と活用

    佐藤ちひろ

    第5回 糖鎖分科会 

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    Event date: 2006

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  53. ミクログリア細胞におけるポリシアル酸構造の発現.

    佐藤ちひろ

    文科省特定領域研究 糖鎖によるタンパク質と分子複合体の機能調節 第四回公開シンポジウム 

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    Event date: 2006

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  54. Hsp70のN型糖鎖結合活性の証明とそのシャペロン活性における役割.

    原田陽一郎、佐藤ちひろ、北島健:

    第7回関西グライコサイエンスフォーラム 

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    Event date: 2006

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  55. 哺乳類細胞におけるデアミノノイラミン酸(KDN)代謝調節機構の解析

    郷 慎司、佐藤ちひろ、北島 健

    第26回 日本糖質学会年会 

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    Event date: 2006

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  56. ウニ精子鞭毛局在flagellasialinのユニークな糖鎖修飾の精子運動性における重要性

    宮田真路, 佐藤ちひろ, 久美田紘信, 鳥山 優, 北島 健

    第77回日本動物学会大会 

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    Event date: 2006

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  57. メダカ初期胚における糖鎖抗原3D11の発現と役割

    北島 健、原 弘明、足立朋子、佐藤ちひろ:

    第77回日本動物学会大会 

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    Event date: 2006

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  58. Polysialic acid on microglia cell and its clearance after LPS-induced activation.

    Sialoglycoscience 2006 

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    Event date: 2006

    Language:English   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  59. Discovery of polysialic acid in microglia. Expression changes of α2,8-polysialyltransferses during activation of a mouse microglia cell line. International conference

    5th International Symposium on Glycosyltransferases 

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    Event date: 2006

    Language:English   Presentation type:Poster presentation  

    Country:Japan  

  60. ミクログリア細胞におけるポリシアル酸構造の存在.

    矢部宇一郎、北島 健、霜田 靖、渡邊 和忠 澤田 誠、佐藤ちひろ

    第78回日本生化学会大会 

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    Event date: 2005

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  61. An essential role of carbohydrate-enriched membrane microdomains (rafts) in gastrulation of medaka fish, Oryzias latipes. International conference

    Gordon Research Conference on Glycobiology 

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    Event date: 2005

    Language:English   Presentation type:Oral presentation (invited, special)  

  62. Milk CD36 Hannah Research Institute International conference

    Nagoya University Joint Workshop on Animal Biotechnology 

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    Event date: 2005

    Language:English   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  63. 糖タンパク質の多様なポリシアル酸修飾の意義を探る.

    佐藤ちひろ

    文科省特定領域研究 糖鎖によるタンパク質と分子複合体の機能調節 第三回夏期シンポジウム 

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    Event date: 2005

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  64. Significance of membrane microdomains in the carbohydrate-dependent cell-cell interactions during early development of medaka fish.

    The 2nd Japan-The Netherlands Glycobiology Symposium. 

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    Event date: 2005

    Language:English   Presentation type:Oral presentation (invited, special)  

  65. Biosynthesis and subcellular localiztion of deaminoneuraminic acid (KDN) in mammalian cells. International conference

    The 18th International Symposium on Glycoconjugates. 

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    Event date: 2005

    Language:English   Presentation type:Poster presentation  

  66. Demonstration of N-glycan-binding activity of HSP70 and its importance in the chaperoning activity.

    The 18th International Symposium on Glycoconjugates. 

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    Event date: 2005

    Language:English   Presentation type:Poster presentation  

  67. Development of a chemical method for detecting a unique sialic acid, cyclic neuraminic acid, in naturally-occurring sialyl glycoconjugates. International conference

    Pacifichem 

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    Event date: 2005

    Language:English   Presentation type:Oral presentation (invited, special)  

  68. マウス乳糖タンパク質CD36上のポリシアル酸の乳腺発達段階における発現解析

    矢部宇一郎、佐藤ちひろ、北島 健

    日本農芸化学会2005年度大会 

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    Event date: 2005

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  69. 新規ポリシアル酸含有糖タンパク質flagellasialinの発見、構造および機能.

    宮田真路, 佐藤ちひろ, 久美田紘信, 鳥山 優, Victor D. Vacquier, 北島 健

    第25回日本糖質学会 

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    Event date: 2005

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  70. ジ・オリゴシアル酸を含有するマウス血清糖タンパク質の同定

    安川然太、佐藤ちひろ、北島 健

    第3回糖鎖科学名古屋拠点 若手の力 

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    Event date: 2005

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  71. メダカ初期胚における細胞膜ラフト形成の重要性とその役割.

    足立朋子、佐藤ちひろ、北島 健

    第3回糖鎖科学名古屋拠点 若手の力 

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    Event date: 2005

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  72. Chain Length Diversity of Sialic Acids and Its Biological Significance

    SATO Chihiro

    Trends in Glycoscience and Glycotechnology 

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    Event date: 2004.9

    Language:English   Presentation type:Oral presentation (general)  

    Sialic acids are commonly present as monosialyl residues at the non-reducing terminal end of glycoconjugates. In some cases, &alpha;2&rarr;8 linked di/oligosialic acid chains with DP 2 or 3 sialic acid residues are common structural units of gangliosides, and involved in various biological processes, such as cell adhesion, cell differentiation, signal transduction, and surface expression of stage specific antigen. In contrast, little attention has been paid to the occurrence and functions of such short sialyl di/oligomers on glycoproteins, while polysialic acid (DP&ge;8) in embryonic NCAM has been extensively studied as a regulator of cell adhesion in neurogenesis. As analytical methods to detect di/oligosialic acid structures have been improved, several glycoproteins containing di/oligo/polysialic acid chains have been identified. It is thus hypothesized that these di/oligosialic acid residues on glycoproteins may have similar important functions in common with those proposed for the gangliosides or may have new functions. Currently, several studies showing the importance of di/oligosialic acid-containing glycoproteins have emerged. In this review, recent advances in such studies of di/oligosialic acid residues on glycoproteins, including analytical methods, occurrence, biosynthetic pathways, and functions, are described.

    DOI: 10.4052/tigg.16.331

    Scopus

  73. Preface for the Special Issue Entitled &ldquo;New Waves in Sialobioscience&rdquo;

    Sato Chihiro

    Trends in Glycoscience and Glycotechnology 

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    Event date: 2004.9

    Language:English   Presentation type:Oral presentation (general)  

    DOI: 10.4052/tigg.16.291

    Scopus

  74. Occurrence of a novel 8-O-sulfated Neu5Ac-capped a2,8-linked polyNeu5Ac chains in the sea urchin sperm flagella glycoprotein

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    Event date: 2004

    Language:Japanese   Presentation type:Poster presentation  

    Country:Japan  

  75. 食物抗原の腸管吸収:吸収された卵アルブミンの末梢血中での量的変動と質的変化

    松原 毅、青木直人、佐藤ちひろ、灘野大太、松田 幹

    日本農芸化学会2004年度大会 

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    Event date: 2004

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  76. 膜リン脂質結合タンパク質MFG-E8の退縮期乳腺での発現変動

    中谷 肇、青山康二、青木直人、佐藤ちひろ、灘野大太、松田 幹

    日本農芸化学会2004年度大会 

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    Event date: 2004

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  77. Study on the uptake of deaminoneuraminic acid (KDN) by mammalian cells

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    Event date: 2004

    Language:Japanese   Presentation type:Poster presentation  

    Country:Japan  

  78. Glycolipid- and phospholipid-binding activity of heat shock protein 70

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    Event date: 2004

    Language:Japanese   Presentation type:Poster presentation  

    Country:Japan  

  79. 鳥類卵膜マトリクスの in vitro での再形成:糖タンパク質ZP1とZPCの特異的会合

    奥村裕紀、青木直人、佐藤ちひろ、灘野大太、松田幹

    日本農芸化学会2004年度大会 

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    Event date: 2004

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  80. Expression of the disialic acid structure on glycoproteins during T cell activation International conference

    Sialobiology 2004 

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    Event date: 2004

    Language:English   Presentation type:Poster presentation  

  81. Identification and characterization of a new heat shock protein-like lectin on the egg surface of sea urchin International conference

    21st International Lectin Meeting 

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    Event date: 2004

    Language:English   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  82. 動物細胞におけるシアル酸の取り込み、分解に関する研究

    郷慎司、佐藤ちひろ、北島健

    日本農芸化学会2004年度大会 

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    Event date: 2004

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  83. ウニ卵局在精子結合タンパク質 (SBP)のレクチン活性

    原田陽一郎、佐藤ちひろ、北島健

    日本農芸化学会2004年度大会 

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    Event date: 2004

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  84. Roles of carbohydrate-mediated lipid raft-lipid raft interactions in early development of medaka, Oryzias latipes

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    Event date: 2003

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  85. ジおよびオリゴシアル酸含有糖タンパク質の構造と機能

    佐藤ちひろ

    糖鎖科学名古屋拠点第1回「若手の力」フォーラム 

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    Event date: 2003

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  86. シアル酸の重合度多様性の発見とその生物学的意義の解明

    佐藤ちひろ

    第24回日本糖質学会年会(奨励賞講演) 

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    Event date: 2003

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  87. Discovery of two new polysialoglycoproteins in human and sea urchin. Linkage and length diversities of polysialic acid in animal glycoproteins. International conference

    Annual Conference of the Society for Glycobiology 

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    Event date: 2003

    Language:English   Presentation type:Oral presentation (invited, special)  

  88. ヒトおよびマウスのミルクにオリゴ・ポリシアル酸構造が存在した

    矢部 宇一郎、佐藤ちひろ、松田 幹、北島 健

    日本農芸化学会2003年度大会 

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    Event date: 2003

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  89. シアル酸合成能をもつ昆虫細胞の検索とその存在証明

    郷 慎司、佐藤ちひろ、池田素子、小林迪弘、松田 幹、北島 健

    日本農芸化学会2003年度大会; 

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    Event date: 2003

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  90. ヒトミルクにおける新しいポリシアル酸含有糖タンパク質CD36の発見

    矢部宇一郎, 佐藤ちひろ, 松田 幹, 北島 健

    第24回日本糖質学会年会 

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    Event date: 2003

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  91. 新規糖アミノ酸α-C-マンノシルトリプトファンの合成と生物機能解析

    西川俊夫、梶井重男、和田享子、石川 幸、磯部 稔、佐藤ちひろ、安川然太、北島 健

    第24回日本糖質学会年会; 

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    Event date: 2003

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  92. ウニ精子鞭毛における新規ポリシアル酸含有糖タンパク質の発見とその糖鎖構造解析

    宮田真路, 佐藤ちひろ, 鳥山 優、北島 健

    第74回日本動物学会大会 

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    Event date: 2003

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  93. メダカ初期胚発生における細胞膜マイクロドメイン形成の重要性の検討

    足立朋子、佐藤ちひろ、北島 健

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    Event date: 2003

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  94. Characterization of a disialic acid-containing glycoprotein expressed on neural cells

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    Event date: 2003

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  95. ジシアル酸含有糖タンパク質の神経細胞(Neuro2A)における発現

    佐藤ちひろ、松田 幹、北島 健

    第75回日本生化学会年会 

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    Event date: 2002

    Language:Japanese   Presentation type:Poster presentation  

    Country:Japan  

  96. Sialic acid-mediated interactions at sea urchin International conference

    The Third Pan-Pacific Conference on Sialoglycoscience and Other Novel Forms of Glycosylation 

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    Event date: 2002

    Language:English   Presentation type:Oral presentation (invited, special)  

  97. マンノース経口投与マウスの臓器におけるKDN合成

    郷 慎司、仲田大輔、佐藤ちひろ、松田 幹、北島 健

    日本農芸化学会2002年度大会 

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    Event date: 2002

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  98. ニジマスポリシアル酸転移酵素(PST)のcDNAクローニングと発現解析

    朝比奈慎二、佐藤ちひろ、松田 幹、北島 健

    日本農芸化学会2002年度大会 

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    Event date: 2002

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  99. ニジマス卵巣におけるO-型糖鎖上のポリシアル酸構造の生合成に関与するシアル酸転移酵素のクローニング

    朝比奈慎二、松野みどり、佐藤ちひろ、北島 健

    第23回日本糖質学会年会 

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    Event date: 2002

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  100. メダカ初期胚における細胞膜ドメイン(ラフト)の単離と細胞間相互作用における機能

    足立朋子、佐藤ちひろ、松田 幹、戸谷一英、村田健臣、碓氷泰一、北島 健

    第73回 日本動物学会年会 

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    Event date: 2002

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  101. 精子膜マイクロドメインに局在する糖脂質と糖タンパク質に共通の糖鎖エピトープの機能

    宮田真路、前橋絵梨、太田薫、佐藤ちひろ、松田幹、北島 健

    第73回 日本動物学会年会 

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    Event date: 2002

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  102. Sialic acid-dependent raft-raft interactions at fertilization International conference

    XVIth International Symposium on glycoconjugates 

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    Event date: 2001.8

    Language:English   Presentation type:Oral presentation (invited, special)  

  103. Identification of a new diSia-containing glycoprotein (adipoQ) in bovine and mouse sera International conference

    XVIth International Symposium on Glycoconjugates 

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    Event date: 2001.7

    Language:English   Presentation type:Poster presentation  

    Congress Center The Hague Netherlands

  104. 哺乳動物に見いだされるオリゴ・ポリシアル酸含有糖タンパク質ファミリーの構造と機能

    佐藤ちひろ

    第43回FCCAセミナー 

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    Event date: 2001.7

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    "グランシップ, 静岡"

  105. "Identification of adipo Q, an adipocyte-specific glycoprotein, as a new oligosialic acid-containing glycoprotein and a possible substrate for the a2,8-sialyltransferase III (ST8SiaIII)." International conference

    "International Symposium on Protein Traffic, Glycosylation, and Human Health" 

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    Event date: 2001.5

    Language:English   Presentation type:Poster presentation  

  106. 精子ラフト-卵ラフト間相互作用の存在とその受精における重要性

    北島 健、前橋絵梨、太田 薫、佐藤ちひろ、松田 幹、鳥山 優

    日本動物学会第72回大会 

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    Event date: 2001

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  107. ニジマス卵巣&#61537;2,6-シアル酸転移酵素のcDNAクローニングと組み換え体酵素の   性質

    朝比奈慎二、佐藤ちひろ、松田 幹、北島 健

    2001年度農芸化学大会 

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    Event date: 2001

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  108. 血清糖タンパク質におけるジシアル酸およびオリゴシアル酸残基の 同定および生合成

    佐藤ちひろ、安川然太、本田直輝、松田 幹、北島 健

    第22回日本糖質学会年会 

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    Event date: 2001

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  109. Roles of Membrane Microdomains (Rafts) in Sperm-Egg Interaction. International conference

    Second Canada-Japan Bilateral Workshop on Human Reproduction and Reproductive Biology 

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    Event date: 2001

    Language:English   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  110. 糖タンパク質糖鎖におけるジおよびオリゴシアル酸の構造と機能

    佐藤ちひろ

    糖鎖物質設計シンポジウム 

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    Event date: 2000.8

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  111. "Expression and glycosylation of a major, carbohydrate-rich, cortical alveolus glycoprotein (hyosophorin) during oogenesis of medaka fish, Oryzias latipes" International conference

    3rd International Symposium of the Molecular and Cellular Biology of the Egg and Embryo 

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    Event date: 2000.8

    Language:English   Presentation type:Poster presentation  

  112. Di- and oligosialic acids as novel carbohydrate epitopes in leukocytes International conference

    7th Conference on human leukocyte differentiation antigens 

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    Event date: 2000.6

    Language:English   Presentation type:Poster presentation  

  113. Membrane microdomains are involved in carbohydrate-dependent sperm-egg interaction in sea urchin International conference

    3rd International Symposium of the Molecular and Cellular Biology of the Egg and Embryo 

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    Event date: 2000.1

    Language:English   Presentation type:Oral presentation (invited, special)  

  114. 脂肪細胞の分化に伴うジおよびオリゴシアル酸含有糖タンパク質とα2,8-シアル酸 転移酵素の発現変化

    佐藤ちひろ、松田 幹、北島 健

    第73回日本生化学会大会 

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    Event date: 2000

    Language:Japanese   Presentation type:Poster presentation  

    Country:Japan  

  115. Lipid raft on gametic cells as a functional domain for sperm-egg interaction coupled with signal transduction International conference

    The International Symposium on Development and Fertilization of Sea Urchin and Marine Invertebrates 

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    Event date: 1999.12

    Language:English   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  116. Egg receptor for sperm binds to gangliosides in the low density detergent-insoluble membrane (LD-DIM) domain of the sperm surface. Possible involvement of the LD-DIM in sperm-egg binding coupled with signal transduction during fertilization. International conference

    XVth International Symposium on Glycoconjugates 

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    Event date: 1999.8

    Language:English   Presentation type:Poster presentation  

    Country:Japan  

  117. Glycosphingolipid enriched microdomain on gametic cells as functional domain for sperm-egg interaction coupled with signal transduction International conference

    Gordon Research Conference on Fertilization and Activation of the Development 

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    Event date: 1999.6

    Language:English   Presentation type:Oral presentation (invited, special)  

  118. 哺乳動物におけるオリゴシアル酸含有糖タンパク質の普遍的存在および分化に伴う発現変化

    佐藤ちひろ、松田 幹、北島 健

    第72回日本生化学会大会 

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    Event date: 1999

    Language:Japanese   Presentation type:Poster presentation  

    Country:Japan  

  119. オリゴシアル酸含有糖タンパク質の新しい化学的および免疫化学的解析法の開発と哺乳動物細胞における普遍的存在の証明

    "佐藤ちひろ,本田直輝,太田薫,福岡秀幸,井上貞子,松田幹,北島健"

    第71回日本生化学会大会 

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    Event date: 1998.10

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  120. "Expression of a2,8-linked oligosialic acid-containing glycoproteins in mammalian cells, depending on development, cell differentiation, and overexpression of polysialyltransferase genes." International conference

    XIXth International Carbohydrate Symposium 

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    Event date: 1998.8

    Language:English   Presentation type:Oral presentation (invited, special)  

  121. Frequent occurrence of oligosialyl units in vertebrate glycoproteins as evidenced by new chemical and immunochemical methods. International conference

    XIVth International Symposium on Glycoconjugates 

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    Event date: 1997.9

    Language:English   Presentation type:Oral presentation (invited, special)  

  122. ブタ脳に存在するオリゴN-アセチルノイラミン酸含有糖タンパク質

    佐藤ちひろ、福岡秀幸、松田幹、井上貞子、井上康男、北島 健

    第70回日本生化学会大会 

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    Event date: 1997

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  123. 哺乳動物細胞におけるオリゴ/ポリN-グリコリルノイラミン酸含有糖タンパク質の存在証明

    佐藤ちひろ、北島 健、古川清、井上貞子、井上康男

    第19回糖質シンポジウム 

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    Event date: 1997

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  124. オリゴシアリル化は哺乳動物組織における糖タンパク質糖鎖の普遍的修飾反応である

    北島 健、佐藤ちひろ、福岡秀幸、松田幹、越野理香、小林一清、井上貞子、井上康男

    第19回糖質シンポジウム 

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    Event date: 1997

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  125. 哺乳動物におけるKDN-およびpoly-α2,8-KDN含有複合糖質の免疫組織化学的および化学・生化学的同定とその生物学的意義

    井上貞子、北島 健、金森審子、佐藤ちひろ、Marti Ziak、Baoxi Qu、Xulei Zuo、Christian Zuber、Jurgen Roth、井上康男

    第18回糖質シンポジウム 

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    Event date: 1996

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  126. α2→8-結合N-グリコリルノイラミン酸(Neu5Gc)オリゴマー構造を特異的に認識する単クローン抗体の調製と特異性の決定

    佐藤ちひろ、北島 健、井上康男、井上貞子

    第68回日本生化学会大会 

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    Event date: 1995

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  127. Unique structures and structural chains of the cortical alveolar-derived glycopolyproteins in fish eggs upon fertilization and during early development. International conference

    3rd International Marine Biotechnology Conference 

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    Event date: 1994

    Language:English   Presentation type:Oral presentation (invited, special)  

  128. 合成ポリシアロ糖脂質を用いたポリシアル酸微量検出法の開発

    佐藤ちひろ、北島 健、井上康男、井上貞子

    第66回日本生化学会大会 

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    Event date: 1993

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  129. Diversity of polysialic acids structure in polysialoglycoproteins of Salmonidae fish eggs: Occurrence of α2→8-linked Poly(Neu5Ac), Poly(Neu5Gc), Poly(KDN), Poly(Neu5Ac,Neu5Gc), and their partially O-acetylated derivatives. International conference

    International Symposium on Polysialic Acid 

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    Event date: 1992.8

    Language:English   Presentation type:Oral presentation (invited, special)  

  130. Polysialic acids in animals. International conference

    6th the Federation of Asian and Oceanian Biochemists Congress 

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    Event date: 1992

    Language:English   Presentation type:Oral presentation (invited, special)  

  131. ポリシアロ糖タンパク質における α2→8 結合ポリシアル酸構造の多様性

    佐藤ちひろ、北島 健、田沢一朗、井上康男、井上貞子

    第65回日本生化学会大会 

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    Event date: 1992

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  132. Sialic acid-dependent raft-raft interactions at fertilization

    XVIth International Symposium on glycoconjugates  2001.8.19 

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    Language:English   Presentation type:Oral presentation (invited, special)  

  133. Preface for the Special Issue Entitled &ldquo;New Waves in Sialobioscience&rdquo; International conference

    Sato Chihiro

    Trends in Glycoscience and Glycotechnology  2004.9  FCCA(Forum: Carbohydrates Coming of Age)

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    Language:English   Presentation type:Oral presentation (general)  

  134. Polysialic acid is not a negative regulator any longer in neurogenesis and neuronal functions: A new finding of its binding to a particular group of neurotransmitters.

    Ken Kitajima, Ryo Isomura, Chihiro Sato

    Gordon Research Conference on Glycobiology,  2009.1.18 

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  135. One-pot Enzymatic Synthesis of CMP-[14C]deaminoneuraminic Acid (KDN).

    Yuko Nakajima, Akiko Fujita, Chihiro Sato, Ken Kitajima

    SialoGlyco Conference 2008  2008.7 

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  136. Occurrence of the Cyclic Sialic Acid in Mammalian Cells and Tissues As Demonstrated by Chemical Methods.

    K. Kitajima, S.Iwata, N. Kawakami, C. Sato, H. Ando, H. Ohno, H. Ishida, M. Kiso, O. Taguchi, R. Kannagi

    SialoGlyco Conference 2008  2008.7 

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    Language:English   Presentation type:Oral presentation (invited, special)  

  137. Novel insights into the novel binding sites of the sialic acid biding Ig-like lectin-7 (Siglec-7) gained by in silico docking studies.

    A one-day symposium on Sialyltransferases, Functional Glycomics, and Genomics of Zebrafish,  2008.11.18 

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    Language:English   Presentation type:Symposium, workshop panel (nominated)  

  138. Membrane microdomains are involved in carbohydrate-dependent sperm-egg interaction in sea urchin

    3rd International Symposium of the Molecular and Cellular Biology of the Egg and Embryo  2000.1.1 

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  139. mAb.A2B5の抗原特異性の決定とマウス脳発達段階におけるmAb.A2B5反応性オリゴシアル酸含有糖タンパク質の発現解析

    猪子恵未, 西浦祐二, 田中浩士, 高橋孝志, 北島健, 佐藤ちひろ

    第81回日本生化学会大会  2008.12.9 

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  140. Lipid raft on gametic cells as a functional domain for sperm-egg interaction coupled with signal transduction

    The International Symposium on Development and Fertilization of Sea Urchin and Marine Invertebrates  1999.12.1 

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  141. In vitro and in silico docking studies of heat shock protein (Hsp) 70 with sulfatide.

    Nao Yamakawa, Yoichiro Harada, Chihiro Sato, Gerard Vergoten, Ken Kitajima

    24th International Carbohydrate Symposium,  2008.7.27 

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  142. In silico binding studies predict novel sialic acid binding sites on the sialic acid binding Ig-like lectin-7 (Siglec-7)

    Nao Yamakawa, Chihiro Sato, Paul R. Crocker, Gérard Vergoten, Ken Kitajima: I

    SialoGlyco Conference 2008  2008.7 

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  143. Identification of the nuclear export signals that regulate the intracellular localization of the mouse CMP-sialic acid synthetase.

    Akiko Fujita, Chihiro Sato, Ken Kitajima

    SialoGlyco Conference 2008  2008.7 

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  144. Identification of a new diSia-containing glycoprotein (adipoQ) in bovine and mouse sera

    XVIth International Symposium on Glycoconjugates  2001.7.19 

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    Congress Center The Hague Netherlands

  145. Glycosphingolipid enriched microdomain on gametic cells as functional domain for sperm-egg interaction coupled with signal transduction

    Gordon Research Conference on Fertilization and Activation of the Development  1999.6.1 

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  146. Frequent occurrence of oligosialyl units in vertebrate glycoproteins as evidenced by new chemical and immunochemical methods.

    XIVth International Symposium on Glycoconjugates  1997.9.27 

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  147. Egg receptor for sperm binds to gangliosides in the low density detergent-insoluble membrane (LD-DIM) domain of the sperm surface. Possible involvement of the LD-DIM in sperm-egg binding coupled with signal transduction during fertilization.

    XVth International Symposium on Glycoconjugates  1999.8.1 

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  148. Di- and oligosialic acids as novel carbohydrate epitopes in leukocytes

    7th Conference on human leukocyte differentiation antigens  2000.6.1 

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  149. Complex formation between polysialic acid and brain derived neurotrophic factor (BDNF).

    Chihiro Sato, Yukihiro Kanato, Ken Kitajima

    24th International Carbohydrate Symposium,  2008.7.27 

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  150. Chain Length Diversity of Sialic Acids and Its Biological Significance International conference

    SATO Chihiro

    Trends in Glycoscience and Glycotechnology  2004.9  FCCA(Forum: Carbohydrates Coming of Age)

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    Sialic acids are commonly present as monosialyl residues at the non-reducing terminal end of glycoconjugates. In some cases, &alpha;2&rarr;8 linked di/oligosialic acid chains with DP 2 or 3 sialic acid residues are common structural units of gangliosides, and involved in various biological processes, such as cell adhesion, cell differentiation, signal transduction, and surface expression of stage specific antigen. In contrast, little attention has been paid to the occurrence and functions of such short sialyl di/oligomers on glycoproteins, while polysialic acid (DP&ge;8) in embryonic NCAM has been extensively studied as a regulator of cell adhesion in neurogenesis. As analytical methods to detect di/oligosialic acid structures have been improved, several glycoproteins containing di/oligo/polysialic acid chains have been identified. It is thus hypothesized that these di/oligosialic acid residues on glycoproteins may have similar important functions in common with those proposed for the gangliosides or may have new functions. Currently, several studies showing the importance of di/oligosialic acid-containing glycoproteins have emerged. In this review, recent advances in such studies of di/oligosialic acid residues on glycoproteins, including analytical methods, occurrence, biosynthetic pathways, and functions, are described.

  151. Brain-derived neurotrophic factor binds to polysialic acid directly depending on degree of polymerization.

    Yukihiro Kanato, Ken Kitajima, Chihiro Sato

    SialoGlyco Conference 2008  2008.7 

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  152. "Identification of adipo Q, an adipocyte-specific glycoprotein, as a new oligosialic acid-containing glycoprotein and a possible substrate for the a2,8-sialyltransferase III (ST8SiaIII)."

    "International Symposium on Protein Traffic, Glycosylation, and Human Health"  2001.5.12 

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  153. "Expression of a2,8-linked oligosialic acid-containing glycoproteins in mammalian cells, depending on development, cell differentiation, and overexpression of polysialyltransferase genes."

    XIXth International Carbohydrate Symposium  1998.8.1 

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  154. "Expression and glycosylation of a major, carbohydrate-rich, cortical alveolus glycoprotein (hyosophorin) during oogenesis of medaka fish, Oryzias latipes"

    3rd International Symposium of the Molecular and Cellular Biology of the Egg and Embryo  2000.8.1 

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  155. 魚類CMP-シアル酸合成酵素の構造と性質 International conference

    藤田明子, 安川裕子, 佐藤ちひろ, 北島健

    日本農芸化学会2009年度大会  2009.3.29 

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  156. 酸性多糖と神経栄養因子との相互作用解析.

    金戸幸弘, 北島健, 佐藤ちひろ

    第81回日本生化学会大会  2008.12.9 

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  157. 糖タンパク質糖鎖におけるジおよびオリゴシアル酸の構造と機能 International conference

    佐藤ちひろ

    糖鎖物質設計シンポジウム  2000.8.1 

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  158. 昆虫細胞由来シアル酸9-リン酸合成酵素の構造と基質特異性の解析 International conference

    濱口香代, 郷慎司, 佐藤ちひろ, 小林迪弘, 池田素子, 北島健

    第81回日本生化学会大会  2008.12.9 

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  159. 抗ジシアル酸抗体が認識する急性期タンパク質の同定.

    安川然太, 北島健, 佐藤ちひろ

    第81回日本生化学会大会  2008.12.9 

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  160. 哺乳類シアロ糖タンパク質のデアミノノイラミン酸 (KDN) 修飾糖タンパク質への改変. International conference

    中島優子, 藤田明子, 佐藤ちひろ, 北島健

    第81回日本生化学会大会  2008.12.9 

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  161. 哺乳動物に見いだされるオリゴ・ポリシアル酸含有糖タンパク質ファミリーの構造と機能 International conference

    佐藤ちひろ

    第43回FCCAセミナー  2001.7.14 

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    "グランシップ, 静岡"

  162. 化学的検出法を用いた天然におけるサイクリックシアル酸の局在検索. International conference

    岩田章子, 佐藤ちひろ, 安藤弘宗, 石田秀治, 木曽真, 神奈木玲児, 北島健

    第81回日本生化学会大会  2008.12.9 

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  163. ラクタム化シアル酸誘導体の合成とその分解反応に関する研究. International conference

    大野ひろみ, 安藤弘宗, 川上信彦, 岩田章子, 佐藤ちひろ, 北島健, 石田秀治, 木曽真

    日本農芸化学会2009年度大会  2009.3.29 

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  164. ポリシアル酸は特定の神経伝達物質と相互作用する.

    磯村遼, 北島健, 佐藤ちひろ

    第81回日本生化学会大会  2008.12.9 

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  165. ポリシアル酸の新機能 International conference

    佐藤ちひろ

    第6回日本糖鎖科学コンソーシアムシンポジウム-糖鎖研究と他領域との統合-  2008.12.3 

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  166. ポリシアル酸と脳由来神経栄養因子の相互作用の解析 International conference

    金戸幸弘, 北島健, 佐藤ちひろ

    日本農芸化学会2009年度大会  2009.3.29 

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  167. ポリシアル酸とFGF-2の相互作用の解析. International conference

    小野明日香, 金戸幸弘, 北島 健, 佐藤ちひろ

    日本農芸化学会2009年度大会  2009.3.29 

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  168. オリゴシアル酸含有糖タンパク質の新しい化学的および免疫化学的解析法の開発と哺乳動物細胞における普遍的存在の証明 International conference

    佐藤ちひろ, 本田直輝, 太田薫, 福岡秀幸, 井上貞子, 松田幹, 北島健

    第71回日本生化学会大会  1998.10.16 

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▼display all

Works 8

  1. Specific labeling and chemical analysis of sialic acid and sialyloligo/polymers

    2011

  2. Enzyme assay of polysialyltransferase (

    2011

  3. Carbohydrate analysis by gas-liquid chromatography

    2011

  4. Enzyme assay of polysialyltransferase (

    2011

  5. Sialic acid and evolution

    2006

  6. Function of polysialic acid in nervus system

    2004

  7. Essentials of glycobiology

    2003

  8. Biosynthesis of oligo/polysialic acid

    2002

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Research Project for Joint Research, Competitive Funding, etc. 4

  1. ポリシアル酸と相互作用する分子の解明

    2010.4 - 2011.3

    公益信託 林女性自然科学研究者研究助成基金 

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    Grant type:Competitive

  2. 脳機能に関わるシアル酸重合体と相互作用する分子の同定とその応用基盤の確立

    2007.4

    加藤記念研究助成 

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    Grant type:Competitive

  3. ミクログリア細胞におけるポリシアル酸の存在とその機能解析

    2006

  4. 神経形成を制御する新規糖鎖オリゴシアル酸の作用機構の解明

    2003.4 - 2004.3

    水谷科学振興財団2003年度研究助成 

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    Grant type:Competitive

KAKENHI (Grants-in-Aid for Scientific Research) 22

  1. 免疫細胞レクチンシグレックの新規リガンド結合部位の証明と新しい免疫制御機構の解明

    Grant number:21H02425  2021.4 - 2025.3

    文部科学省  科研費  B

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    Authorship:Principal investigator  Grant type:Competitive

  2. Molecular mechanism of new cellular functions mediated by polysialic acid

    Grant number:15K06995  2015.4 - 2018.3

    SATO Chihiro

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    Grant amount:\4940000 ( Direct Cost: \3800000 、 Indirect Cost:\1140000 )

    Polysiali acid (polySia/PSA) is present in brain and is involved in learning, memory and social interaction. So far, we have been demonstrated that polySia is involved in brain functions not only due to its large exclusion volume but also due to molecule binding properties. In this research, we demonstrated that polySia chains show characteristic features towards molecule-binding properties via biosynthetic enzymes, ST8SIA2 and ST8SIA4.

  3. Synthesis of chemical probes containing di/oligosialialic acids and their biological evaluation related to innate immunity

    Grant number:26282209  2014.4 - 2017.3

    Tanaka Hiroshi

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    In this report, we first investigated that partially unprotected sialyl donors underwent a-selective glycosidation without use of an acetonitrile effect. To demonstrate the utility of the method, a straight forward synthesis of α(2,9) disialosides containing N-acetyl and/or N-glycolyl groups was achieved by using the two N-acetyl and N-glycolyl sialyl donors. Next we successfully prepared Glycopolymers possessing disialic acids with a narrow with narrow polydisparities from glycosylated monomers via π-allyl nickel catalyzed coordinating polymerization. A biological evaluation of the glycopolymers and revealed that the synthetic fluoroecent-labelled glycopolymers cause the Siglec-7-GD3 interaction to dissociate in the micromolar concentration range. Finally we prepared the synthesis of dextran derivative possessing disialic acids via polymer reactions. The glycopolymer was able to release Siglec7 from Siglec7-GD3 complex possibly.

  4. 精神疾患に関与するポリシアル酸転移酵素の機能解析

    Grant number:26110708  2014.4 - 2016.3

    新学術領域研究(研究領域提案型)

    佐藤 ちひろ

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    Authorship:Principal investigator 

    Grant amount:\9360000 ( Direct Cost: \7200000 、 Indirect Cost:\2160000 )

    本研究は統合失調症などの精神疾患に関連するポリシアル酸転移酵素ST8SIA2の機能解析が目的であり、本研究では報告のある精神疾患患者に有意なSNPsや変異に関して、それらの酵素がもたらす産物であるポリシアル酸の構造および機能解析を行った。具体的にはプロモーターに存在するrSNPに関してはプロモータ解析および遺伝子のマイクロアレイの解析を各種細胞を用いて行い、ある転写因子によって細胞特異的にST8SIA2の発現異常が起こる可能性を示した。翻訳領域に存在しアミノ酸置換を伴うSNPの解析から、疾患型が合成するポリシアル酸が損なわれ、特にBDNFの結合やproBDNFの結合性が正常型より損なわれることがわかった。また翻訳領域に存在しアミノ酸置換を伴わないsSNPは、コドン使用頻度によりST8SIA2の翻訳速度が変化し、ポリシアル酸の生合成に影響を与える可能性が示唆された。イントロンに存在するiSNPは、特に双極性障害型のSNPに関して、マウスの神経細胞およびヒトHEK細胞においてST8SIA2の合成が促進され、polySiaが過剰発現することが明らかになった。ST8SIA2の過剰発現の影響を調べるために子宮内エレクトロポレーション法により、ST8SIA2およびST8SIA4の遺伝子導入を行ったところ、ニューロンの移動がMockに比べて変化した。以上のことより、ST8SIA2遺伝子発現は細胞特異的に発現が厳密に制御されており、それに伴いpolySiaの構造が損なわれることが分かった。つまり、polySia構造は厳密に構造が制御されており、それによって神経作用因子であるBDNF、FGF2, proBDNFの結合性を制御することなどで、正常な神経機能を果たすことが考えられた。
    27年度が最終年度であるため、記入しない。
    27年度が最終年度であるため、記入しない。

  5. Novel regulatory mechanism for intracellular calcium ion concentration by membrane microdomain-localized glycans in sperm

    Grant number:25292216  2013.4 - 2016.3

    Kitajima Ken

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    Many studies using glycan-related probes have reported the importance of glycan chains in fertilization. However, glycan-related gene-modified studies have told that glycans are not always essential for a successful fertilization. It is thus a currently important subject to re-evaluate molecular mechanisms for glycan-involved events at fertilization. To solve the subject, we focused on the "membrane microdomain" that is molecular complex of proteins and lipids with highly concentrated glycans on plasma membrane. In this study, we demonstrated the followings: (1) Glycan-enriched GPI-anchor peptide on the sperm membrane microdomains ubiquitously occurs not only in sea urchin and pig, but also avians and amphibians that have never been shown to contain such GPI-anchored molecules before; (2) These glycans are involved in sperm motility through regulating the intracellular calcium ion concentration in sperm.

  6. 微小糖鎖空間における酸性多糖が織りなす神経制御機構の解明

    2012.4 - 2014.3

    科学研究費補助金  新学術領域

    佐藤ちひろ

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  7. 細胞表面酸性多糖が織りなす微小糖鎖空間による神経機能調節機構の解明

    Grant number:24110506  2012.4 - 2014.3

    新学術領域研究(研究領域提案型)

    佐藤 ちひろ

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    Authorship:Principal investigator 

    Grant amount:\7150000 ( Direct Cost: \5500000 、 Indirect Cost:\1650000 )

    ポリシアル酸はシアル酸が8-400残基縮重合した構造の総称で、胎児脳の神経細胞接着分子(NCAM)を特異的に修飾する。成体脳ではその大部分が消失するが、海馬や嗅球といった神経新生の盛んな領域に発現が継続している。ポリシアル酸の機能として反接着作用が知られていたが、我々はポリシアル酸が自身に神経機能を制御する分子、BDNF、FGF2,ドーパミンなどを結合し、その機能を制御しうることを明らかにしてきている。本研究では、(1) polySiaによる神経作用因子の保持・放出機構の解明、(2) poySiaの"質と量"を制御する生合成機構の解明、(3) polySiaと統合失調症などの精神疾患との関わりの解明をすすめること目指した。(1)に関してはpolySiaが、自身の質と量を調節することにより、神経作用因子を保持状態を制御することがわかった。またその受容体への受け渡し機構には、より高親和性受容体への直接受け渡し、共受容体への間接的な受け渡し、polySia鎖の構造変化による受け渡しなど、さまざまな機構が働いていることが初めて明らかになった。(2)では、polySiaの質と量を制御する機構としてポリシアル酸転移酵素(ST8SIA2)に着目し、その変異が生合成する機構を一部明らかにした。(3)においては、統合失調症患者で明らかになったST8SIA2のSNP-7およびSNP-9の解析を正常型とあわせて行うことにより、その生合成酵素の産物の不全がpolySia鎖の不全を引き起こし、ひいては分子結合性における不全がおきることによるシグナル伝達の不具合がおこることを示した。以上の結果より、本研究において、polySia鎖がつくり出す細胞膜近傍における空間において、自身の分子結合性により細胞機能を制御していること、このpolySia鎖の生合成は高度の制御されていることが強く示唆された。
    25年度が最終年度であるため、記入しない。
    25年度が最終年度であるため、記入しない。

  8. Regulation of neural functions via polySia and FGF2 interaction

    Grant number:23570133  2011 - 2013

    SATO Chihiro

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    Grant amount:\5460000 ( Direct Cost: \4200000 、 Indirect Cost:\1