Updated on 2021/04/06

写真a

 
SATO, Chihiro
 
Organization
Institute for Glyco-core Research Professor
Graduate School of Bioagricultural Sciences Professor
Graduate School of Bioagricultural Sciences Professor
Graduate School
Graduate School of Bioagricultural Sciences
Title
Professor
Contact information
メールアドレス

Degree 1

  1. Ph D ( 1997.3   The University of Tokyo ) 

Research Interests 2

  1. Glycobiology, acidic sugar, sialic acid, polysialic acid, neuron, brain, gametic cell, raft, mental disorder, schizophrenia, autism, bipolar disorder

  2. bipolar disorder

Research Areas 3

  1. Life Science / Functional biochemistry  / Functional Biochemistry

  2. Life Science / Structural biochemistry  / Structured Biochemistry

  3. Life Science / Applied biochemistry

Current Research Project and SDGs 3

  1. Structure and Function of Di/Oligo/Polysialic acid

  2. 疾患関連糖鎖の構造と機能

  3. 疾患に関わる糖鎖プローブの開発と利用

Research History 10

  1. Nagoya University   iGCORE   Professor

    2021.1

  2. Nagoya University   Integrated Glyco-Biomedical Research Center   Director in General

    2021.1

  3. Nagoya University   Graduate School of Bioagricultural Sciences   Professor

    2019.10

  4. Nagoya University   Bioscience and Biotechnology Center   Associate professor

    2007.4 - 2019.9

  5. Associate Professor, Bioscience and Biotechnology Center, Nagoya University

    2007.4 - 2018.9

      More details

    Country:Japan

  6. Bioscience and Biotechnology Center, Nagoya University/Associate Professor

    2005.2 - 2007.3

      More details

    Country:Japan

  7. 名古屋大学大学院生命農学研究科 助手

    2001.9 - 2005.1

      More details

    Country:Japan

  8. 名古屋大学生物分子応答研究センター 研究機関研究員

    2001.4 - 2001.8

      More details

    Country:Japan

  9. 日本学術振興会 特別研究員

    1998.4 - 2001.3

      More details

    Country:Japan

  10. 日本学術振興会 特別研究員

    1996.4 - 1998.3

      More details

    Country:Japan

▼display all

Education 3

  1. The University of Tokyo   Graduate School of Science   Department of Biophysics and Biochemistry

    1994.4 - 1997.3

      More details

    Country: Japan

  2. The University of Tokyo

    1992.4 - 1994.3

  3. The University of Tokyo   Faculty of Science   Department of Biophysics and Biochemistry

    1988.4 - 1992

      More details

    Country: Japan

Professional Memberships 8

  1. 日本生化学会   評議員

    2021.1

  2. 日本糖質学会   評議員

    2005.4

  3. 日本生化学会   中部支部会幹事

    2012.9 - 2014.8

  4. 日本糖鎖科学コンソーシアム(JCGG)   評議員

    2018.4

  5. 日本農芸化学会

  6. 糖鎖科学名古屋拠点   事務局

    2003.4

  7. ASBMB

  8. 日本神経科学会

▼display all

Committee Memberships 8

  1. 日本生化学会   評議員  

    2021.1   

      More details

    Committee type:Academic society

  2. 日本糖質学会   評議員  

    2005.4   

      More details

    Committee type:Academic society

  3. 日本糖鎖科学コンソーシアム (JCGG)   評議員  

    2018.4   

  4. 日本生化学誌   企画委員  

    2017.9   

      More details

    Committee type:Academic society

  5. 日本農芸化学会 化学と生物   編集員  

    2016.4 - 2021.3   

  6. 日本生化学会中部支部会   幹事  

    2012.9 - 2014.8   

      More details

    Committee type:Academic society

  7. 日本農芸化学会   代議員  

    2009.4 - 2011.3   

      More details

    Committee type:Academic society

  8. 糖鎖科学中部拠点   事務局  

    2003.4   

      More details

    Committee type:Other

▼display all

Awards 4

  1. 日本糖質学会奨励賞

    2003   日本糖質学会  

     More details

    Country:Japan

  2. 第1回日本農芸化学女性研究者賞

    2017.3   日本農芸化学会  

     More details

    Award type:Award from Japanese society, conference, symposium, etc.  Country:Japan

  3. 第3回日本糖質学会ポスター賞

    1999  

     More details

    Country:Japan

  4. Complex Formation of a Brain-Derived Neurotrophic Factor and Glycosaminoglycans.

    2010.3   Biosci. Biotech. and Biochem.  

     More details

    Country:Japan

 

Papers 336

  1. Evolutionary conservation of human ketodeoxynonulosonic acid production is independent of sialoglycan biosynthesis. Reviewed International coauthorship

    Kawanishi K, Saha S, Diaz S, Vaill M, Sasmal A, Siddiqui SS, Choudhury B, Sharma K, Chen X, Schoenhofen IC, Sato C, Kitajima K, Freeze HH, Münster-Kühnel A, Varki A

    The Journal of clinical investigation   Vol. 131 ( 5 )   2021.3

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Journal of Clinical Investigation  

    DOI: 10.1172/JCI137681

    Web of Science

    Scopus

    PubMed

  2. Identification and functional characterization of a Siglec-7 counter-receptor on K562 cells. Reviewed International coauthorship

    Yoshimura A, Asahina Y, Chang LY, Angata T, Tanaka H, Kitajima K, Sato C

    The Journal of biological chemistry     page: 100477   2021.2

     More details

    Authorship:Last author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.jbc.2021.100477

    PubMed

  3. Polysialylation and disease. Reviewed

    Sato C, Kitajima K

    Molecular aspects of medicine     page: 100892   2020.8

     More details

    Authorship:Lead author, Last author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:Molecular Aspects of Medicine  

    DOI: 10.1016/j.mam.2020.100892

    Scopus

    PubMed

  4. Discovery of a new sialic acid binding region that regulates Siglec-7. Reviewed International coauthorship International journal

    Nao Yamakawa, Yu Yasuda, Atsushi Yoshimura, Ami Goshima, Paul R Crocker, Gérard Vergoten, Yuji Nishiura, Takashi Takahashi, Shinya Hanashima, Kana Matsumoto, Yoshiki Yamaguchi, Hiroshi Tanaka, Ken Kitajima, Chihiro Sato

    Scientific reports   Vol. 10 ( 1 ) page: 8647 - 8647   2020.5

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    Siglec-7 is a human CD33-like siglec, and is localised predominantly on human natural killer (NK) cells and monocytes. Siglec-7 is considered to function as an immunoreceptor in a sialic acid-dependent manner. However, the underlying mechanisms linking sialic acid-binding and function remain unknown. Here, to gain new insights into the ligand-binding properties of Siglec-7, we carried out in silico analysis and site-directed mutagenesis, and found a new sialic acid-binding region (site 2 containing R67) in addition to the well-known primary ligand-binding region (site 1 containing R124). This was supported by equilibrium dialysis, STD-NMR experiments, and inhibition analysis of GD3-binding toward Siglec-7 using synthetic sialoglycoconjugates and a comprehensive set of ganglioside-based glycoconjugates. Our results suggest that the two ligand-binding sites are potentially controlled by each other due to the flexible conformation of the C-C' loop of Siglec-7.

    DOI: 10.1038/s41598-020-64887-4

    Web of Science

    Scopus

    PubMed

  5. PSA-NCAM Colocalized with Cholecystokinin-Expressing Cells in the Hippocampus Is Involved in Mediating Antidepressant Efficacy. Reviewed

    Yamada J, Sato C, Konno K, Watanabe M, Jinno S

    The Journal of neuroscience : the official journal of the Society for Neuroscience   Vol. 40 ( 4 ) page: 825 - 842   2020.1

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Journal of Neuroscience  

    DOI: 10.1523/JNEUROSCI.1779-19.2019

    Web of Science

    Scopus

    PubMed

  6. Effect of expression alteration in flanking genes on phenotypes of St8sia2-deficient mice. Reviewed International journal

    Keisuke Ikegami, Kazumasa Saigoh, Atsuko Fujioka, Mamoru Nagano, Ken Kitajima, Chihiro Sato, Satoru Masubuchi, Susumu Kusunoki, Yasufumi Shigeyoshi

    Scientific reports   Vol. 9 ( 1 ) page: 13634 - 13634   2019.9

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 2 (ST8SIA2) synthesizes polysialic acid (PSA), which is essential for brain development. Although previous studies reported that St8sia2-deficient mice that have a mixed 129 and C57BL/6 (B6) genetic background showed mild and variable phenotypes, the reasons for this remain unknown. We hypothesized that this phenotypic difference is caused by diversity in the expression or function of flanking genes of St8sia2. A genomic polymorphism and gene expression analysis in the flanking region revealed reduced expression of insulin-like growth factor 1 receptor (Igf1r) on the B6 background than on that of the 129 strain. This observation, along with the finding that administration of an IGF1R agonist during pregnancy increased litter size, suggests that the decreased expression of Igf1r associated with ST8SIA2 deficiency caused lethality. This study demonstrates the importance of gene expression level in the flanking regions of a targeted null allele having an effect on phenotype.

    DOI: 10.1038/s41598-019-50006-5

    Web of Science

    Scopus

    PubMed

  7. Functional roles of ST8SIA3-mediated sialylation of striatal dopamine D<sub>2</sub> and adenosine A<sub>2A</sub> receptors. Reviewed International coauthorship

    Lin CY, Lai HL, Chen HM, Siew JJ, Hsiao CT, Chang HC, Liao KS, Tsai SC, Wu CY, Kitajima K, Sato C, Khoo KH, Chern Y

    Translational psychiatry   Vol. 9 ( 1 ) page: 209   2019.8

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1038/s41398-019-0529-z

    Web of Science

    Scopus

    PubMed

  8. Acute stress-induced change in polysialic acid levels mediated by sialidase in mouse brain. Reviewed

    Abe C, Yi Y, Hane M, Kitajima K, Sato C

    Scientific reports   Vol. 9 ( 1 ) page: 9950   2019.7

     More details

    Authorship:Last author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1038/s41598-019-46240-6

    Web of Science

    Scopus

    PubMed

  9. Structure and Function of di/oligo/polysialic Acid (Polymerized Sialyl Structure) Invited Reviewed

    Sato Chihiro

    TRENDS IN GLYCOSCIENCE AND GLYCOTECHNOLOGY   Vol. 31 ( 181 ) page: SE32 - SE35   2019.7

     More details

    Authorship:Lead author, Last author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:Trends in Glycoscience and Glycotechnology  

    <p>Sialic acid (Sia) is known to present as a monosialyl residue at a non-reducing terminal end of glycan in nature. In some cases, Sias occur as polymerized sialyl structures, di/oligo/polysialic acids (diSia/oligoSia/polySias). Here, we focus on the structure, function and biological significance of diSia/oligoSia/polySias. Following the definition of di/oligo/polySia structures and establishment of chemical and immunochemical detection methods, we analyzed these structures and their function based on a set of working hypotheses. So far, the biological meanings of degree of polymerization (DP) were shown and they lead to the change the concept of polySia. In addition, this research is applicable in the study of diseases such as mental disorder and cancer. However, research on the biological significance of the diversity in di/oligo/polySia structures is still ongoing.</p>

    DOI: 10.4052/tigg.1915.2SE

    Web of Science

    Scopus

    CiNii Article

  10. Sialic Acids in Neurology. Reviewed

    Sato C, Kitajima K

    Advances in carbohydrate chemistry and biochemistry   Vol. 76   page: 1 - 64   2019

     More details

    Authorship:Lead author, Corresponding author   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/bs.accb.2018.09.003

    Web of Science

    Scopus

    PubMed

  11. Glycans in Nervous System Reviewed

    Sato Chihiro, Gu Jianguo, Fukuda Tomohiko, Kadomatsu Kenji, Ikenaka Kazuhiro

    GLYCOSCIENCE: BASIC SCIENCE TO APPLICATIONS: INSIGHTS FROM THE JAPAN CONSORTIUM FOR GLYCOBIOLOGY AND GLYCOTECHNOLOGY (JCGG)     page: 209 - 219   2019

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Glycoscience: Basic Science to Applications: Insights from the Japan Consortium for Glycobiology and Glycotechnology (JCGG)  

    DOI: 10.1007/978-981-13-5856-2_12

    Web of Science

    Scopus

  12. Exploring the Impact of Ketodeoxynonulosonic Acid in Host-Pathogen Interactions Using Uptake and Surface Display by Nontypeable Haemophilus influenzae. Reviewed International coauthorship

    Saha S, Coady A, Sasmal A, Kawanishi K, Choudhury B, Yu H, Sorensen RU, Inostroza J, Schoenhofen IC, Chen X, Münster-Kühnel A, Sato C, Kitajima K, Ram S, Nizet V, Varki A

    mBio   Vol. 12 ( 1 ) page: 1 - 24   2021.1

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:mBio  

    DOI: 10.1128/mBio.03226-20

    Scopus

    PubMed

  13. The conserved arginine residue in all siglecs is essential for Siglec-7 binding to sialic acid. Reviewed

    Yoshimura A, Hatanaka R, Tanaka H, Kitajima K, Sato C

    Biochemical and biophysical research communications   Vol. 534   page: 1069 - 1075   2021.1

     More details

    Authorship:Last author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:Biochemical and Biophysical Research Communications  

    DOI: 10.1016/j.bbrc.2020.10.023

    Web of Science

    Scopus

    PubMed

  14. The expression of sulfated sialic acids (SiaS) is dynamically regulated in mammalian cells

    Kitajima Ken, Ertunc Nursah, Sato Chihiro

    GLYCOBIOLOGY   Vol. 30 ( 12 ) page: 1066 - 1067   2020.12

     More details

    Publishing type:Research paper (scientific journal)  

    Web of Science

  15. Comparative Studies of Polysialic Acids Derived from Five Different Vertebrate Brains. Reviewed

    Yang Y, Murai R, Takahashi Y, Mori A, Hane M, Kitajima K, Sato C

    International journal of molecular sciences   Vol. 21 ( 22 ) page: 1 - 22   2020.11

     More details

    Authorship:Last author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:International Journal of Molecular Sciences  

    DOI: 10.3390/ijms21228593

    Web of Science

    Scopus

    PubMed

  16. Sialic acid sulfation is induced by the antibiotic treatment in mammalian cells. Reviewed

    Ertunc N, Sato C, Kitajima K

    Bioscience, biotechnology, and biochemistry   Vol. 84 ( 11 ) page: 2311 - 2318   2020.11

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Bioscience, Biotechnology and Biochemistry  

    DOI: 10.1080/09168451.2020.1792763

    Web of Science

    Scopus

    PubMed

  17. Combinational Analyses with Multiple Methods Reveal the Existence of Several Forms of Polysialylated Neural Cell Adhesion Molecule in Mouse Developing Brains. Reviewed

    Mori A, Yang Y, Takahashi Y, Hane M, Kitajima K, Sato C

    International journal of molecular sciences   Vol. 21 ( 16 ) page: 1 - 20   2020.8

     More details

    Authorship:Last author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:International Journal of Molecular Sciences  

    DOI: 10.3390/ijms21165892

    Web of Science

    Scopus

    PubMed

  18. Identification and characterization of a novel, versatile sialidase from a Sphingobacterium that can hydrolyze the glycosides of any sialic acid species at neutral pH. Reviewed

    Iwaki Y, Matsunaga E, Takegawa K, Sato C, Kitajima K

    Biochemical and biophysical research communications   Vol. 523 ( 2 ) page: 487 - 492   2020.3

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Biochemical and Biophysical Research Communications  

    DOI: 10.1016/j.bbrc.2019.12.079

    Web of Science

    Scopus

    PubMed

  19. N-linked mannose glycoconjugates on shrimp thrombospondin, pmTSP-II, and their involvement in the sperm acrosome reaction. Reviewed International coauthorship

    Timklay W, Magerd S, Sato C, Somrit M, Watthammawut A, Senarai T, Weerachatyanukul W, Kitajima K, Asuvapongpatana S

    Molecular reproduction and development   Vol. 86 ( 4 ) page: 440 - 449   2019.4

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1002/mrd.23122

    Web of Science

    Scopus

    PubMed

  20. Structural Biology of Glycans Reviewed

    Sato C, Kato K, Yamaguchi Y, Kohda D, Kato R, Suzuki GN K, Kikuchi K, Hirai G, Kizuka Y, Tanaka K, Nakashima Y, Setou M

    Glycoscience: Basic Science to Applications     page: 35 - 63   2019

     More details

    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

  21. Structure and Function of di/oligo/polysialic Acid (Polymerized Sialyl Structure) Invited Reviewed

    Sato Chihiro

    Trends in Glycoscience and Glycotechnology   Vol. 31 ( 181 ) page: SJ32 - SJ35   2019

     More details

    Authorship:Lead author, Corresponding author   Language:Japanese   Publishing type:Research paper (scientific journal)   Publisher:FCCA(Forum: Carbohydrates Coming of Age)  

    <p>Sialic acid (Sia) is known to present as a monosialyl residue at a non-reducing terminal end of glycan in nature. In some cases, Sias occur as polymerized sialyl structures, di/oligo/polysialic acids (diSia/oligoSia/polySias). Here, we focus on the structure, function and biological significance of diSia/oligoSia/polySias. Following the definition of di/oligo/polySia structures and establishment of chemical and immunochemical detection methods, we analyzed these structures and their function based on a set of working hypotheses. So far, the biological meanings of degree of polymerization (DP) were shown and they lead to the change the concept of polySia. In addition, this research is applicable in the study of diseases such as mental disorder and cancer. However, research on the biological significance of the diversity in di/oligo/polySia structures is still ongoing.</p>

    DOI: 10.4052/tigg.1915.2SJ

    CiNii Article

  22. Preparation and Properties of a Polycyclic p-Quinodimethane with Two Oxygen Bridges and its Radical Cation in Comparison with the Isomeric o-Quinodimethane.

    Sato C, Suzuki S, Okada K, Kozaki M

    Chemistry, an Asian journal   Vol. 13 ( 23 ) page: 3729 - 3736   2018.12

     More details

    Publishing type:Research paper (scientific journal)  

    DOI: 10.1002/asia.201801269

    PubMed

  23. Systems glycomics of adult zebrafish identifies organ-specific sialylation and glycosylation patterns. Reviewed

    Yamakawa N, Vanbeselaere J, Chang LY, Yu SY, Ducrocq L, Harduin-Lepers A, Kurata J, Aoki-Kinoshita KF, Sato C, Khoo KH, Kitajima K, Guerardel Y

    Nature communications   Vol. 9 ( 1 ) page: 4647   2018.11

     More details

    Publishing type:Research paper (scientific journal)  

    DOI: 10.1038/s41467-018-06950-3

    Web of Science

    Scopus

    PubMed

  24. Mental disorders and an acidic glycan-from the perspective of polysialic acid (PSA/polySia) and the synthesizing enzyme, ST8SIA2. Reviewed

    Sato C, Hane M

    Glycoconjugate journal   Vol. 35 ( 4 ) page: 353 - 373   2018.8

     More details

    Publishing type:Research paper (scientific journal)  

    DOI: 10.1007/s10719-018-9832-9

    Web of Science

    Scopus

    PubMed

  25. 酸性糖鎖ポリシアル酸の新機能の発見とその応用展開 Reviewed

    佐藤 ちひろ

    化学と生物   Vol. 56   page: 422 - 431   2018

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  26. New Functions and Applications of an Acidic Glycan, Polysialic Acid: Repulsive and Attractive Fields of Polysialic Acid

    SATO Chihiro

    KAGAKU TO SEIBUTSU   Vol. 56 ( 6 ) page: 422 - 431   2018

     More details

    Publishing type:Research paper (scientific journal)   Publisher:Japan Society for Bioscience, Biotechnology, and Agrochemistry  

    DOI: 10.1271/kagakutoseibutsu.56.422

    CiNii Article

  27. Positive selection on schizophrenia-associated ST8SIA2 gene in post-glacial Asia. Reviewed

    Fujito NT, Satta Y, Hane M, Matsui A, Yashima K, Kitajima K, Sato C, Takahata N, Hayakawa T

    PloS one   Vol. 13 ( 7 ) page: e0200278   2018

     More details

    Publishing type:Research paper (scientific journal)  

    DOI: 10.1371/journal.pone.0200278

    Web of Science

    Scopus

    PubMed

  28. Different properties of polysialic acids synthesized by the polysialyltransferases ST8SIA2 and ST8SIA4

    Airi Mori, Masaya Hane, Yuki Niimi, Ken Kitajima, Chihiro Sato

    GLYCOBIOLOGY   Vol. 27 ( 9 ) page: 834 - 846   2017.9

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:OXFORD UNIV PRESS INC  

    Polysialic acid (polySia) is mainly found as a modification of neural cell adhesion molecule (NCAM) in whole embryonic brains, as well as restricted areas of adult vertebrate brains, including the hippocampus. PolySia shows not only repulsive effects on NCAM-involved cell-cell interactions due to its bulky and hydrated properties, but also attractive effects on the interaction with neurologically active molecules, which exerts a reservoir function. Two different polysialyltransferases, ST8SIA2 and ST8SIA4, are involved in the synthesis of polySia chains; however, to date, the differences of the properties between polySia chains synthesized by these two enzymes remain unknown. In this study, to clarify this point, we first prepared polySia-NCAMs from HEK293 cells stably expressing ST8SIA4 and ST8SIA2, or ST8SIA2 (SNP-7), a mutant ST8SIA2 derived from a schizophrenia patient. The conventional sensitive chemical and immunological characterizations showed that the quantity and quality (structural features) of polySia are not so much different between ST8SIA4-and ST8SIA2-synthesized ones, apart from those of ST8SIA2 (SNP-7). Then, we assessed the homophilic and heterophilic interactions mediated by polySia-NCAM by adopting a surface plasmon resonance measurement as an in vitro analytical method. Our novel findings are as follows: (i) the ST8SIA2-and ST8SIA4-synthesized polySia-NCAMs exhibited different attractive and repulsive effects than each other; (ii) both polySia-and oligoSia-NCAMs synthesized by ST8SIA2 were able to bind polySia-NCAMs; (iii) the polySia-NCAM synthesized by a ST8SIA2 (SNP-7) showed markedly altered attractive and repulsive properties. Collectively, polySia-NCAM is suggested to simultaneously possess both attractive and repulsive properties that are highly regulated by the two polysialyltransferases.

    DOI: 10.1093/glycob/cwx057

    Web of Science

    Scopus

    PubMed

  29. Chemical Synthesis and Evaluation of a Disialic Acid-Containing Dextran Polymer as an Inhibitor for the Interaction between Siglec 7 and Its Ligand

    Sho Yamaguchi, Atsushi Yoshimura, Yu Yasuda, Airi Mori, Hiroshi Tanaka, Takashi Takahashi, Ken Kitajima, Chihiro Sato

    CHEMBIOCHEM   Vol. 18 ( 13 ) page: 1194 - 1203   2017.7

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:WILEY-V C H VERLAG GMBH  

    A new sialic acid (Sia)-containing glycopolymer-a fluorescent probe with high-density disialic acid (diSia) on the surface of polysaccharide dextran (diSia-Dex)-was synthesized as a key molecule to regulate the Sia recognition lectins, Siglecs, that are involved in the immune system. According to our original methods, diSia was synthesized by alpha-selective sialylation, and a dextran template possessing terminal acetylenes and amino groups was prepared. A diSia and a fluorescent molecule were subsequently introduced to surface-modified dextran by Huisgen reaction and amidation, respectively. The modulatory activity of Siglec7 was evaluated by using synthetic probes. DiSia-Dex showed high binding avidity toward Siglec7, with a KD value of 5.87 x 10(-10) M, and a high inhibitory activity for the interaction between Siglec7 and a ligand (GD3), with a IC50 value of 1.0 nM. Notably, diSia-Dex was able to release Siglec7 from the pre-existing Siglec7-GD3 complex, possibly due to its unique properties of a slow dissociation rate and a high association rate. Together, these data show that diSia-Dex can be widely applicable as a modulator of Siglec7 functions.

    DOI: 10.1002/cbic.201600694

    Web of Science

    Scopus

    PubMed

  30. Alpha-2 macroglobulin as a region-specific secretory protein in male reproductive tract, and its dynamics during sperm transit toward the female spermatheca in the blue crab

    Thanyaporn Senarai, Rapeepun Vanichviriyakit, Shinji Miyata, Chihiro Sato, Prapee Sretarugsa, Wattana Weerachatyanukul, Ken Kitajima

    MOLECULAR REPRODUCTION AND DEVELOPMENT   Vol. 84 ( 7 ) page: 585 - 595   2017.7

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:WILEY  

    A 250-kDa protein was isolated from fluid in the middle spermatic duct (MSD) of the blue crab (Portunus pelagicus). N-terminal and partial amino acid sequences revealed that this MSD-specific protein is highly similar to the plasma-enriched protein Alpha-2 macroglobulin (alpha 2M). The P. pelagicus ortholog (Pp alpha 2M) is a large glycoprotein possessing mannose and N-acetylglucosamine residues. Pp alpha 2M mRNA was detected in the spermatic duct, androgenic gland, and hematopoietic tissue, whereas the protein was primarily observed in the apical cytoplasm of MSD epithelium and in the matrix of the acrosome of MSD sperm; distally within spermatic duct, Pp alpha 2M was lost from the sperm membrane but remained in the sperm acrosome. These results suggest that Pp alpha 2M is expressed and glycosylated in the epithelium of spermatic ducts, secreted into MSD fluid, taken up by sperm in the MSD, and removed from the surface of sperm during its transit towards the female spermatheca. Given that Pp alpha 2M also exhibits protease inhibitor activity, we hypothesize that acrosome localized Pp alpha 2M may suppress premature acrosome reaction during post-testicular sperm maturation in this crab.

    DOI: 10.1002/mrd.22817

    Web of Science

    Scopus

    PubMed

  31. Chlorpromazine Increases the Expression of Polysialic Acid (PolySia) in Human Neuroblastoma Cells and Mouse Prefrontal Cortex

    Chikara Abe, Saki Nishimura, Airi Mori, Yuki Niimi, Yi Yang, Masaya Hane, Ken Kitajima, Chihiro Sato

    INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES   Vol. 18 ( 6 )   2017.5

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:MDPI AG  

    The neural cell adhesion molecule (NCAM) is modified by polysialic acid (polySia or PSA) in embryonic brains. In adult brains, polySia modification of NCAM is only observed in restricted areas where neural plasticity, remodeling of neural connections, or neural generation is ongoing although the amount of NCAM remains unchanged. Impairments of the polySia-expression and several single nucleotide polymorphisms (SNPs) of the polysialyltransferase (polyST) ST8SIA2 gene are reported to be associated with schizophrenia and bipolar disorder. Chlorpromazine (CPZ) is well-known as an agent for treating schizophrenia, and our hypothesis is that CPZ may affect the polySia expression or the gene expression of polySTs or NCAM. To test this hypothesis, we analyzed the effects of CPZ on the expression of polySia-NCAM on human neuroblastoma cell line, IMR-32 cells, by immunochemical and chemical methods. Interestingly, the cell surface expression of polySia, especially those with lower chain lengths, was significantly increased on the CPZ-treated cells, while mRNAs for polySTs and NCAM, and the amounts of total polySia-NCAM remained unchanged. The addition of brefeldin A, an inhibitor of endocytosis, suppressed the CPZ-induced cell surface polySia expression. In addition, polySia-NCAM was also observed in the vesicle compartment inside the cell. All these data suggest that the level of cell surface expression of polySia in IMR-32 is highly regulated and that CPZ changes the rate of the recycling of polySia-NCAM, leading to the up-regulation of polySia-NCAM on the cell surface. We also analyzed the effect of CPZ on polySia-expression in various brain regions in adult mice and found that CPZ only influenced the total amounts of polySia-NCAM in prefrontal cortex. These results suggest a brain-region-specific effect of CPZ on the expression of total polySia in mouse brain. Collectively, anti-schizophrenia agent CPZ consistently up-regulates the expression polySia at both cellular and animal levels.

    DOI: 10.3390/ijms18061123

    Web of Science

    Scopus

    PubMed

  32. Altered expression of ganglioside GM3 molecular species and a potential regulatory role during myoblast differentiation

    Shinji Go, Shiori Go, Lucas Veillon, Maria Grazia Ciampa, Laura Mauri, Chihiro Sato, Ken Kitajima, Alessandro Prinetti, Sandro Sonnino, Jin-ichi Inokuchi

    JOURNAL OF BIOLOGICAL CHEMISTRY   Vol. 292 ( 17 ) page: 7040 - 7051   2017.4

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC  

    Gangliosides (sialic acid-containing glycosphingolipids) help regulate many important biological processes, including cell proliferation, signal transduction, and differentiation, via formation of functional microdomains in plasma membranes. The structural diversity of gangliosides arises from both the ceramide moiety and glycan portion. Recently, differing molecular species of a given ganglioside are suggested to have distinct biological properties and regulate specific and distinct biological events. Elucidation of the function of each molecular species is important and will provide new insights into ganglioside biology. Gangliosides are also suggested to be involved in skeletal muscle differentiation; however, the differential roles of ganglioside molecular species remain unclear. Here we describe striking changes in quantity and quality of gangliosides (particularly GM3) during differentiation of mouse C2C12 myoblast cells and key roles played by distinct GM3 molecular species at each step of the process.

    DOI: 10.1074/jbc.M116.771253

    Web of Science

    Scopus

    PubMed

  33. Diverse subcellular localizations of the insect CMP-sialic acid synthetases

    Wu Di, Akiko Fujita, Kayo Hamaguchi, Philippe Delannoy, Chihiro Sato, Ken Kitajima

    GLYCOBIOLOGY   Vol. 27 ( 4 ) page: 329 - 341   2017.4

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:OXFORD UNIV PRESS INC  

    The occurrence and biological importance of sialic acid (Sia) and its metabolic enzymes in insects have been studied using Drosophila melanogaster. The most prominent feature of D. melanogaster CMP-Sia synthetase (DmCSS) is its Golgi-localization, contrasted with nuclear localization of vertebrate CSSs. However, it remains unclear if the Golgi-localization is common to other insect CSSs and why it happens. To answer these questions, Aedes aegypti (mosquito) CSS (AaCSS) and Tribolium castaneum (beetle) CSS (TcCSS) were cloned and characterized for their activity and subcellular localization. Our new findings show: (1) AaCSS and TcCSS share a common overall structure with DmCSS in terms of evolutionarily conserved motifs and the absence of the C-terminal domain typical to vertebrate CSSs; (2) when expressed in mammalian and insect cells, AaCSS and TcCSS showed in vivo and in vitro CSS activities, similar to DmCSS. In contrast, when expressed in bacteria, they lacked CSS activity because the N-terminal hydrophobic region appeared to induce protein aggregation; (3) when expressed in Drosophila S2 cells, AaCSS and TcCSS were predominantly localized in the ER, but not in the Golgi. Surprisingly, DmCSS was mainly secreted into the culture medium, although partially detected in Golgi. Consistent with these results, the N-terminal hydrophobic regions of AaCSS and TcCSS functioned as a signal peptide to render them soluble in the ER, while the N-terminus of DmCSS functioned as a membrane-spanning region of type II transmembrane proteins whose cytosolic KLK sequence functioned as an ER export signal. Accordingly, the differential subcellular localization of insect CSSs are distinctively more diverse than previously recognized.

    DOI: 10.1093/glycob/cww128

    Web of Science

    Scopus

    PubMed

  34. Synthesis of end-functionalized glycopolymers containing α(2,8) disialic acids via π-allyl nickel catalyzed coordinating polymerization and their interaction with Siglec-7. Reviewed

    Ohira S, Yasuda Y, Tomita I, Kitajima K, Takahashi T, Sato C, Tanaka H.

    Chem Commun (Camb).   Vol. 53 ( 3 ) page: 553-556.   2017.1

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  35. Synthesis of end-functionalized glycopolymers containing alpha(2,8) disialic acids via pi-allyl nickel catalyzed coordinating polymerization and their interaction with Siglec-7 Reviewed

    Shuichi Ohira, Yu Yasuda, Ikuyoshi Tomita, Ken Kitajima, Takashi Takahashi, Chihiro Sato, Hiroshi Tanaka

    CHEMICAL COMMUNICATIONS   Vol. 53 ( 3 ) page: 553 - 556   2017.1

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:ROYAL SOC CHEMISTRY  

    An allene monomer containing an alpha(2,8) disialic acid was polymerized by a pi-allyl nickel complex with an azido group to produce end-functional glycopolymers with an excellent polydispersity index. The polymers allowed terminal modification with a fluorescent dye by click chemistry. The glycopolymers can dissociate Siglec-7-GD3 interactions at low concentrations.

    DOI: 10.1039/c6cc07115e

    Web of Science

    Scopus

    PubMed

  36. Releasing Mechanism of Neurotrophic Factors via Polysialic Acid

    C. Sato

    NEUROTROPHINS   Vol. 104   page: 89 - 112   2017

     More details

    Language:English   Publishing type:Part of collection (book)   Publisher:ELSEVIER ACADEMIC PRESS INC  

    Neurotrophins are well-characterized neurologically active molecules in the central nervous system. The regulation of these signaling molecules, which are involved in cell growth, differentiation, and survival, is critical for normal brain function. Among the different types of neurotrophins, brain-derived neurotrophic factor (BDNF) is involved in various brain functions, including memory consolidation, synaptic plasticity, and adult neurogenesis, and is therefore a key molecule for understanding comprehensive brain function and neurodevelopmental and psychiatric diseases. The concentration of BDNF in body fluid is highly related to several neurodevelopmental and psychiatric diseases, including Alzheimer's diseases, depression, schizophrenia, and bipolar disorder. In the present review, the mechanisms by which BDNF is released from secretory vesicles are reviewed, with a particular focus on the recently described glycan-mediated release. In addition, the impact of glycan-mediated BDNF release on psychiatric disorders is also discussed.

    DOI: 10.1016/bs.vh.2016.11.004

    Web of Science

    Scopus

  37. ポリシアル酸転移酵素遺伝子ST8SIA2と精神疾患の関わり Reviewed

    羽根正弥, 北島 健, 佐藤 ちひろ

    生化学   Vol. 89   page: 634 - 643   2017

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  38. Polysialyltransferase ST8SIA2 and psychiatric disorders

    Hane M.

    Seikagaku   Vol. 89 ( 5 ) page: 634 - 643   2017

     More details

    Publishing type:Research paper (scientific journal)   Publisher:Seikagaku  

    DOI: 10.14952/SEIKAGAKU.2017.890634

    Scopus

  39. Diverse subcellular localizations of the insect CMP-sialic acid synthetases. Reviewed

    Di W, Fujita A, Hamaguchi K, Delannoy P, Sato C, Kitajima K.

    Glycobiology.     2016.12

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: doi: 10.1093/glycob/cww128.

  40. The Out of Africa and mental disorders: Adaptation with reduction in STX promoter activity

    Fujito Naoko, Satta Yoko, Hane Masaya, Matsui Atsushi, Yashima Kenta, Kitajima Ken, Sato Chihiro, Takahata Naoyuki, Hayakawa Toshiyuki

    GENES & GENETIC SYSTEMS   Vol. 91 ( 6 ) page: 343 - 343   2016.12

     More details

    Publishing type:Research paper (scientific journal)  

    Web of Science

  41. A Uniquely Human Evolutionary Change in ST8Sia-II Impacts Enzyme Stability and Polysialic Acid Function

    Vaill Michael, Hane Masaya, Naito-Matsui Yuko, Diaz Sandra, Davies Leela, Kitajima Ken, Sato Chihiro, Varki Ajit

    GLYCOBIOLOGY   Vol. 26 ( 12 ) page: 1423 - 1423   2016.12

     More details

    Publishing type:Research paper (scientific journal)  

    Web of Science

  42. Effects of intronic single nucleotide polymorphisms (iSNPs) of a polysialyltransferase, ST8SIA2 gene found in psychiatric disorders on its gene products Reviewed

    Masaya Hane, Ken Kitajima, Chihiro Sato

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   Vol. 478 ( 3 ) page: 1123 - 9   2016.9

     More details

    Publishing type:Research paper (scientific journal)   Publisher:ACADEMIC PRESS INC ELSEVIER SCIENCE  

    Polysialic acid (polySia) is a linear homopolymer of sialic acid and mainly modifies neural cell adhesion molecule. PolySia plays important roles in synapse formation, learning and memory, social behavior and is associated with several diseases. Gene analyses of one of the biosynthetic enzymes for polySia, ST8SIA2, have revealed that several SNPs and genetic variations in the ST8SIA2 gene are associated with several psychiatric disorders; however, the mechanisms underlying these associations remain unknown. Here, we analyzed the effects of two iSNPs of ST8SIA2, rs2168351 and rs3784730, which are associated with bipolar disorder and autism spectrum disorder, respectively, on the expression of mRNA, ST8SIA2 and its final product, polySia in mouse neuroblastoma and human adenocarcinoma cell lines. We found that both iSNPs affected the expression of pre-mRNA and mRNA of ST8SIA2, and altered the cellular levels of ST8SIA2 and polySia. Taken together, these results indicate that impairment of the regulated expression of ST8SIA2 and the resulting downstream effects on gene products by these two iSNPs contribute to the development of these psychiatric disorders. (C) 2016 Elsevier Inc. All rights reserved.

    DOI: 10.1016/j.bbrc.2016.08.079

    Web of Science

    Scopus

    PubMed

  43. Effects of intronic single nucleotide polymorphisms (iSNPs) of a polysialyltransferase, ST8SIA2 gene found in psychiatric disorders on its gene products. Reviewed

    Hane M, Kitajima K, Sato C.

    Biochem Biophys Res Commun.   Vol. 478 ( 3 ) page: 1123-9.   2016.9

     More details

    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: doi: 10.1016/j.bbrc.2016.08.079.

  44. Relationship between ST8SIA2, polysialic acid and its binding molecules, and psychiatric disorders

    Sato C.

    Biochimica et Biophysica Acta - General Subjects   Vol. 1860 ( 8 ) page: 1739 - 1752   2016.8

     More details

    Publishing type:Research paper (scientific journal)   Publisher:Biochimica et Biophysica Acta - General Subjects  

    DOI: 10.1016/j.bbagen.2016.04.015

    Scopus

  45. Relationship between ST8SIA2, polysialic acid and its binding molecules, and psychiatric disorders. Invited Reviewed

    Sato C, Hane M, Kitajima K.

    Biochim Biophys Acta.   Vol. 1860 ( 8 ) page: 1739-52   2016.8

     More details

    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: doi: 10.1016/j.bbagen.2016.04.015.

  46. Relationship between ST8SIA2, polysialic acid and its binding molecules, and psychiatric disorders.

    Sato C, Hane M, Kitajima K

    Biochimica et biophysica acta   Vol. 1860 ( 8 ) page: 1739 - 52   2016.8

     More details

    Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.bbagen.2016.04.015

    PubMed

  47. Relationship between ST8SIA2, polysialic acid and its binding molecules, and psychiatric disorders. Reviewed

    Sato C, Hane M, Kitajima K

    Biochimica et biophysica acta   Vol. 1860 ( 8 ) page: 1739 - 1752   2016.8

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.bbagen.2016.04.015

    PubMed

  48. Relationship between ST8SIA2, polysialic acid and its binding molecules, and psychiatric disorders Reviewed

    Chihiro Sato, Masaya Hane, Ken Kitajima

    BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS   Vol. 1860 ( 8 ) page: 1739 - 1752   2016.8

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:ELSEVIER SCIENCE BV  

    Polysialic acid (polySia, PSA) is a unique and functionally important glycan, particularly in vertebrate brains. It is involved in higher brain functions such as learning, memory, and social behaviors. Recently, an association between several genetic variations and single nucleotide polymorphisms (SNPs) of ST8SIA2/STX, one of two polysialyltransferase genes in vertebrates, and psychiatric disorders, such as schizophrenia (SZ), bipolar disorder (BD), and autism spectrum disorder (ASD), was reported based on candidate gene approaches and genome-wide studies among normal and mental disorder patients. It is of critical importance to determine if the reported mutations and SNPs in ST8SIA2 lead to impairments of the structure and function of polySia, which is the final product of ST8SIA2. To date, however, only a few such forward-directed studies have been conducted. In addition, the molecular mechanisms underlying polySia-involved brain functions remain unknown, although polySia was shown to have an anti-adhesive effect. In this report, we review the relationships between psychiatric disorders and polySia and/or ST8SIA2, and describe a new function of polySia as a regulator of neurologically active molecules, such as brain-derived neurotrophic factor (BDNF) and dopamine, which are deeply involved in psychiatric disorders. This article is part of a Special Issue entitled "Glycans in personalised medicine" Guest Editor: Professor Gordan Lauc. (C) 2016 Elsevier B.V. All rights reserved.

    DOI: 10.1016/j.bbagen.2016.04.015

    Web of Science

  49. Relationship between ST8SIA2, polysialic acid and its binding molecules, and psychiatric disorders

    Sato Chihiro, Hane Masaya, Kitajima Ken

    BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS   Vol. 1860 ( 8 ) page: 1739 - 1752   2016.8

     More details

    Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.bbagen.2016.04.015

    Web of Science

  50. The AMOR Arabinogalactan Sugar Chain Induces Pollen-Tube Competency to Respond to Ovular Guidance. Reviewed International journal

    Akane G Mizukami, Rie Inatsugi, Jiao Jiao, Toshihisa Kotake, Keiko Kuwata, Kento Ootani, Satohiro Okuda, Subramanian Sankaranarayanan, Yoshikatsu Sato, Daisuke Maruyama, Hiroaki Iwai, Estelle Garénaux, Chihiro Sato, Ken Kitajima, Yoichi Tsumuraya, Hitoshi Mori, Junichiro Yamaguchi, Kenichiro Itami, Narie Sasaki, Tetsuya Higashiyama

    Current biology : CB   Vol. 26 ( 8 ) page: 1091 - 7   2016.4

     More details

    Publishing type:Research paper (scientific journal)  

    Precise directional control of pollen-tube growth by pistil tissue is critical for successful fertilization of flowering plants [1-3]. Ovular attractant peptides, which are secreted from two synergid cells on the side of the egg cell, have been identified [4-6]. Emerging evidence suggests that the ovular directional cue is not sufficient for successful guidance but that competency control by the pistil is critical for the response of pollen tubes to the attraction signal [1, 3, 7]. However, the female molecule for this competency induction has not been reported. Here we report that ovular methyl-glucuronosyl arabinogalactan (AMOR) induces competency of the pollen tube to respond to ovular attractant LURE peptides in Torenia fournieri. We developed a method for assaying the response capability of a pollen tube by micromanipulating an ovule. Using this method, we showed that pollen tubes growing through a cut style acquired a response capability in the medium by receiving a sufficient amount of a factor derived from mature ovules of Torenia. This factor, named AMOR, was identified as an arabinogalactan polysaccharide, the terminal 4-O-methyl-glucuronosyl residue of which was necessary for its activity. Moreover, a chemically synthesized disaccharide, the β isomer of methyl-glucuronosyl galactose (4-Me-GlcA-β-(1→6)-Gal), showed AMOR activity. No specific sugar-chain structure of plant extracellular matrix has been identified as a bioactive molecule involved in intercellular communication. We suggest that the AMOR sugar chain in the ovary renders the pollen tube competent to the chemotropic response prior to final guidance by LURE peptides.

    DOI: 10.1016/j.cub.2016.02.040

    Web of Science

    Scopus

    PubMed

  51. .The AMOR Arabinogalactan Sugar Chain Induces Pollen-Tube Competency to Respond to Ovular Guidance. Reviewed

    Mizukami AG, Inatsugi R, Jiao J, Kotake T, Kuwata K, Ootani K, Okuda S, Sankaranarayanan S, Sato Y, Maruyama D, Iwai H, Garénaux E, Sato C, Kitajima K, Tsumuraya Y, Mori H, Yamaguchi J, Itami K, Sasaki N, Higashiyama T

    Curr Biol.   Vol. 26 ( 8 ) page: 1091-7   2016.4

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  52. 2,11-Dibromo-13,14-dimesityl-5,8-dioxapentaphene: A Stable and Twisted Polycyclic System Containing the o-Quinodimethane Skeleton.

    Sato C, Suzuki S, Kozaki M, Okada K

    Organic letters   Vol. 18 ( 5 ) page: 1052 - 5   2016.3

     More details

    Publishing type:Research paper (scientific journal)  

    DOI: 10.1021/acs.orglett.6b00171

    PubMed

  53. Binding Activity of Hsp70 toward Acidic Glycoconjugates:An Unexpected Role of Sugars

    HARADA Yoichiro, SATO Chihiro, KITAJIMA Ken

    KAGAKU TO SEIBUTSU   Vol. 55 ( 1 ) page: 22 - 26   2016

     More details

    Publishing type:Research paper (scientific journal)   Publisher:Japan Society for Bioscience, Biotechnology, and Agrochemistry  

    DOI: 10.1271/kagakutoseibutsu.55.22

    CiNii Article

  54. Spread of reduced activity of STX promoter throughout Great Journey

    Fujito Naoko, Satta Yoko, Hane Masaya, Matsui Atsushi, Kitajima Ken, Sato Chihiro, Hayakawa Toshiyuki

    GENES & GENETIC SYSTEMS   Vol. 90 ( 6 ) page: 379 - 379   2015.12

     More details

    Publishing type:Research paper (scientific journal)  

    Web of Science

  55. Protective effects of polysialic acid on proteolytic cleavage of FGF2 and proBDNF/BDNF.

    Hane M, Matsuoka S, Ono S, Miyata S, Kitajima K, Sato C

    Glycobiology   Vol. 25 ( 10 ) page: 1112 - 24   2015.10

     More details

    Publishing type:Research paper (scientific journal)   Publisher:Glycobiology  

    DOI: 10.1093/glycob/cwv049

    Web of Science

    Scopus

    PubMed

  56. Ganglioside contained in the neuronal tissue-enriched acidic protein of 22 kDa (NAP-22) fraction prepared from the detergent-resistant membrane microdomain of rat brain inhibits the phosphatase activity of calcineurin.

    Kobayashi Y, da Silva R, Kumanogoh H, Miyata S, Sato C, Kitajima K, Nakamura S, Morita M, Hayashi F, Maekawa S

    Journal of neuroscience research   Vol. 93 ( 9 ) page: 1462 - 70   2015.9

     More details

    Publishing type:Research paper (scientific journal)   Publisher:Journal of Neuroscience Research  

    DOI: 10.1002/jnr.23599

    Web of Science

    Scopus

    PubMed

  57. Rapid Trimming of Cell Surface Polysialic Acid (PolySia) by Exovesicular Sialidase Triggers Release of Preexisting Surface Neurotrophin.

    Sumida M, Hane M, Yabe U, Shimoda Y, Pearce OM, Kiso M, Miyagi T, Sawada M, Varki A, Kitajima K, Sato C

    The Journal of biological chemistry   Vol. 290 ( 21 ) page: 13202 - 14   2015.5

     More details

    Publishing type:Research paper (scientific journal)   Publisher:Journal of Biological Chemistry  

    DOI: 10.1074/jbc.M115.638759

    Web of Science

    Scopus

    PubMed

  58. Sulfatide-Hsp70 interaction promotes Hsp70 clustering and stabilizes binding to unfolded protein.

    Harada Y, Sato C, Kitajima K

    Biomolecules   Vol. 5 ( 2 ) page: 958 - 73   2015.5

     More details

    Publishing type:Research paper (scientific journal)   Publisher:Biomolecules  

    DOI: 10.3390/biom5020958

    Web of Science

    Scopus

    PubMed

  59. Discovery, primary, and crystal structures and capacitation-related properties of a prostate-derived heparin-binding protein WGA16 from boar sperm.

    Garénaux E, Kanagawa M, Tsuchiyama T, Hori K, Kanazawa T, Goshima A, Chiba M, Yasue H, Ikeda A, Yamaguchi Y, Sato C, Kitajima K

    The Journal of biological chemistry   Vol. 290 ( 9 ) page: 5484 - 501   2015.2

     More details

    Publishing type:Research paper (scientific journal)   Publisher:Journal of Biological Chemistry  

    DOI: 10.1074/jbc.M114.635268

    Web of Science

    Scopus

    PubMed

  60. Flagellasialin: Highly Glycosylated Gpi-Anchored Protein Involved in Intracellular Ca2+ Regulation in Sea Urchin Sperm Reviewed

    Takeru Kanazawa, Eriko Suzuki, Shinji Miyata, Chihiro Sato, Ken Kitajima

    Glycoscience: Biology and Medicine     page: 883 - 890   2015.1

     More details

    Language:English   Publishing type:Part of collection (book)   Publisher:Springer Japan  

    Very little knowledge has been available on structure and function of heavily glycosylated proteins, whose glycan parts amount to more than 90 % of the whole molecules, mainly because of technical difficulties in detection and determination of structure. In 2004 flagellasialin was discovered as a major cell surface component in the lipid rafts of sea urchin sperm. The extremely high content of glycan chains completely prevented detecting this glycoprotein by conventional protein staining methods, such as Coomassie Brilliant Blue and silver staining. To make this glycoprotein visible, monoclonal antibodies 4F7 and 3G9 were developed as specific probes for detecting glycan chains of flagellasialin. The frequent occurrence of GPI-anchored proteins in lipid rafts is well recognized. Flagellasialin was actually demonstrated to contain a GPI-anchor, based on three lines of experiments. In addition to the computational predictions from the cDNA sequence and the phosphatidylinositolspecific phospholipase C treatment of the sperm membrane fraction, nitrous acid deamination of a flagellasialin-blotted membrane was newly introduced. The immunostaining of flagellasialin on the membrane disappeared after the nitrous acid treatment of the blotted membrane. This method was easy and effective for identifying flagellasialin as a GPI-anchored protein.

    DOI: 10.1007/978-4-431-54841-6_167

    Scopus

  61. Polysialic acid Reviewed

    Chihiro Sato, Ken Kitajima

    Glycoscience: Biology and Medicine     page: 519 - 528   2015.1

     More details

    Language:English   Publishing type:Part of collection (book)   Publisher:Springer Japan  

    Polysialic acid (polySia), particularly α2,8-linked polyNeu5Ac (DP ¼ 8 ~ 400), is a unique polymer of sialic acid (Sia) and modifies neural cell adhesion molecule (NCAM) spatiotemporally in embryonic brains. PolySia is involved in cell migration, neural outgrowth, axonal guidance, synaptic plasticity, and the development of normal neural circuits and neurogenesis due to its anti- adhesive property and spatiotemporal expression. Recent improvements of polySia-detection methods have told us that polySia expression persists in certain areas of the adult brain, such as the olfactory bulb, hippocampus, subventricular zone, thalamus, prefrontal cortex, and amygdala, where neural plasticity, remodeling of neural connections, or neural generation is ongoing. Importantly, several lines of evidence have raised a new concept of polySia function that polySia works as an attractive field that associates with particular ion channels and neurologically active molecules, such as neurotrophic factor (BDNF), growth factor (FGF2), and neurotransmitter (dopamine), to regulate their involved signaling. It has been shown that polySia is involved in learning, memory, circadian rhythm, and social behaviors using polySia-impaired mice. In addition, SNPs and some deletions in genes encoding the polysialyltransferase and NCAM have been reported from patients of schizophrenia, mood disorder and autism. Such impairments of polySia structure derived from polysialyltransferase gene alterations might influence the polySia function not only as the repulsive field but also as the attractive field.

    DOI: 10.1007/978-4-431-54841-6_117

    Scopus

  62. Polysialyltransferase assay Reviewed

    Masaya Hane, Ken Kitajima, Chihiro Sato

    Glycoscience: Biology and Medicine     page: 529 - 534   2015.1

     More details

    Language:English   Publishing type:Part of collection (book)   Publisher:Springer Japan  

    It has been shown that polysialic acid (polySia) is an indispensable component for viability of mouse during development. Especially, it is deeply involved in normal brain function in mouse and human, affecting cell adhesion, cell migration, cell growth, neurogenesis, and synaptogenesis. PolySia is synthesized by two polysialyltransferases, ST8SIA2 and ST8SIA4. Mice deficient in both enzymes show lethality soon after birth. Mice deficient in either ST8SIA2 or ST8SIA4 show significant impairments in learning, memory, circadian rhythm, and social behavior. Interestingly, different phenotypes are often observed between ST8SIA2- and ST8SIA4-deficient mice. Therefore, characterization of these enzymes in vitro and in vivo and precise understanding of the structurefunction relationship of polySia become more and more important. In this chapter, assay procedures for in vitro activity of the polysialyltransferases are focused on to obtain clear results of their in vitro activities that are often said to be very weak compared with other sialyltransferases.

    DOI: 10.1007/978-4-431-54841-6_118

    Scopus

  63. Protective effects of polysialic acid on proteolytic cleavage of FGF2 and proBDNF/BDNF. Reviewed

    Hane M, Matsuoka S, Ono S, Miyata S, Kitajima K, Sato C.

    Glycobiology   Vol. 25   page: 1112-24   2015

     More details

    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

  64. Sulfatide-Hsp70 interaction promotes Hsp70 clustering and stabilizes binding to unfolded protein. Reviewed

    Harada Y, Sato C, Kitajima K.

    Biomolecules.   Vol. 5   page: 958-73   2015

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  65. Ganglioside contained in the neuronal tissue-enriched acidic protein of 22 kDa (NAP-22) fraction prepared from the detergent-resistant membrane microdomain of rat brain inhibits the phosphatase activity of calcineurin. Reviewed

    Kobayashi Y, da Silva R, Kumanogoh H, Miyata S, Sato C, Kitajima K, Nakamura S, Morita M, Hayashi F, Maekawa S.

    J Neurosci Res.   Vol. 93   page: 1462-70   2015

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  66. Rapid Trimming of Cell Surface Polysialic Acid (PolySia) by Exovesicular Sialidase Triggers Release of Preexisting Surface Neurotrophin. Reviewed

    Sumida M, Hane M, Yabe U, Shimoda Y, Pearce OM, Kiso M, Miyagi T, Sawada M, Varki A, Kitajima K, Sato C.

    J Biol Chem.   Vol. 290   page: 13202-14   2015

     More details

    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

  67. Discovery, primary, and crystal structures and capacitation-related properties of a prostate-derived heparin-binding protein WGA16 from boar sperm. Reviewed

    Garénaux E, Kanagawa M, Tsuchiyama T, Hori K, Kanazawa T, Goshima A, Chiba M, Yasue H, Ikeda A, Yamaguchi Y, Sato C, Kitajima K.

    J Biol Chem.   Vol. 290   page: 5484-501   2015

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  68. Sulfatide-Hsp70 interaction promotes Hsp70 clustering and stabilizes binding to unfolded protein. Reviewed

    Harada Y, Sato C, Kitajima K

    Biomolecules.   Vol. 5   page: 958-73   2015

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  69. Advanced Technologies in Sialic Acid and Sialoglycoconjugate Analysis Reviewed

    Ken Kitajima, Nissi Varki, Chihiro Sato

    SIALOGLYCO CHEMISTRY AND BIOLOGY II: TOOLS AND TECHNIQUES TO IDENTIFY AND CAPTURE SIALOGLYCANS   Vol. 367   page: 75 - 103   2015

     More details

    Publishing type:Part of collection (book)   Publisher:SPRINGER INT PUBLISHING AG  

    Although the structural diversity of sialic acid (Sia) is rapidly expanding, understanding of its biological significance has lagged behind. Advanced technologies to detect and probe diverse structures of Sia are absolutely necessary not only to understand further biological significance but also to pursue medicinal and industrial applications. Here we describe analytical methods for detection of Sia that have recently been developed or improved, with a special focus on 9-O-acetylated N-acetylneuraminic acid (Neu5,9Ac), N-glycolylneuraminic acid (Neu5Gc), deaminoneuraminic acid (Kdn), O-sulfated Sia (SiaS), and di-, oligo-, and polysialic acid (diSia/oligoSia/polySia) in glycoproteins and glycolipids. Much more attention has been paid to these Sia and sialoglycoconjugates during the last decade, in terms of regulation of the immune system, neural development and function, tumorigenesis, and aging.

    DOI: 10.1007/128_2013_458

    Web of Science

    Scopus

    PubMed

  70. Ganglioside contained in the neuronal tissue-enriched acidic protein of 22 kDa (NAP-22) fraction prepared from the detergent-resistant membrane microdomain of rat brain inhibits the phosphatase activity of calcineurin. Reviewed

    Kobayashi Y, da Silva R, Kumanogoh H, Miyata S, Sato C, Kitajima K, Nakamura S, Morita M, Hayashi F, Maekawa S

    J Neurosci Res.   Vol. 93   page: 1462-70   2015

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  71. Discovery, primary, and crystal structures and capacitation-related properties of a prostate-derived heparin-binding protein WGA16 from boar sperm. Reviewed

    Garénaux E, Kanagawa M, Tsuchiyama T, Hori K, Kanazawa T, Goshima A, Chiba M, Yasue H, Ikeda A, Yamaguchi Y, Sato C, Kitajima K

    J Biol Chem.   Vol. 290   page: 5484-501   2015

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  72. Protective effects of polysialic acid on proteolytic cleavage of FGF2 and proBDNF/BDNF. Reviewed

    Hane M, Matsuoka S, Ono S, Miyata S, Kitajima K, Sato C

    Glycobiology   Vol. 25   page: 1112-24   2015

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  73. Rapid Trimming of Cell Surface Polysialic Acid (PolySia) by Exovesicular Sialidase Triggers Release of Preexisting Surface Neurotrophin. Reviewed

    Sumida M, Hane M, Yabe U, Shimoda Y, Pearce OM, Kiso M, Miyagi T, Sawada M, Varki A, Kitajima K, Sato C

    J Biol Chem.   Vol. 290   page: 13202-14   2015

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  74. Adaptive evolution of the promoter region of the Sialyltransferase 8B (STX) gene

    Fujito Naoko T., Hayakawa Toshiyuki, Hane Masaya, Kitajima Ken, Sato Chihiro, Satta Yoko

    GENES & GENETIC SYSTEMS   Vol. 89 ( 6 ) page: 327-327   2014.12

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    Web of Science

  75. Tissue-Specific Posttranslational Modification Allows Functional Targeting of Thyrotropin Reviewed

    Keisuke Ikegami, Xiao-Hui Liao, Yuta Hoshino, Hiroko Ono, Wataru Ota, Yuka Ito, Taeko Nishiwaki-Ohkawa, Chihiro Sato, Ken Kitajima, Masayuki Iigo, Yasufumi Shigeyoshi, Masanobu Yamada, Yoshiharu Murata, Samuel Refetoff, Takashi Yoshimura

    CELL REPORTS   Vol. 9 ( 3 ) page: 801 - 10   2014.11

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:CELL PRESS  

    Thyroid-stimulating hormone (TSH; thyrotropin) is a glycoprotein secreted from the pituitary gland. Pars distalis-derived TSH (PD-TSH) stimulates the thyroid gland to produce thyroid hormones (THs), whereas pars tuberalis-derived TSH (PT-TSH) actsonthe hypothalamus to regulate seasonal physiology and behavior. However, it had not been clear how these two TSHs avoid functional crosstalk. Here, we show that this regulation ismediated by tissue-specific glycosylation. Although PT-TSH is released into the circulation, it does not stimulate the thyroid gland. PD-TSH is known to have sulfated biantennary N-glycans, and sulfated TSH is rapidly metabolized in the liver. In contrast, PT-TSH has sialylated multibranched N-glycans; in the circulation, it forms the macro-TSH complex with immunoglobulin or albumin, resulting in the loss of its bioactivity. Glycosylation is fundamental to a wide range of biological processes. This report demonstrates its involvement in preventing functional crosstalk of signaling molecules in the body.

    DOI: 10.1016/j.celrep.2014.10.006

    Web of Science

    Scopus

    PubMed

  76. An anti-schizophrenic drug affects the surface expression of polySia-NCAM in IMR-32 human neuroblastoma cells

    Nishimura Saki, Hane Masaya, Niimi Yuki, Kitajima Ken, Sato Chihiro

    GLYCOBIOLOGY   Vol. 24 ( 11 ) page: 1193-1193   2014.11

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    Web of Science

  77. Critical importance of the surface expression of alpha 2,6-sialosides during early development of medaka fish

    Kitajima Ken, Wu Di, Tajima Katsue, Maruyama Emi, Yasukawa Yuko, Chang Lan-Yi, Taniguchi Yoshihito, Kamei Yasuhiro, Adachi Tomoko, Hashimoto Hisashi, Hibi Masahiko, Fujita Akiko, Sato Chihiro

    GLYCOBIOLOGY   Vol. 24 ( 11 ) page: 1110-1110   2014.11

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    Web of Science

  78. Insight into single nucleotide polymorphisms (SNPs) of the polysialyltransferase ST8SIA2/STX in psychiatric disorders

    Hane Masaya, Nishimura Saki, Hayakawa Toshiyuki, Kitajima Ken, Sato Chihiro

    GLYCOBIOLOGY   Vol. 24 ( 11 ) page: 1206-1206   2014.11

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    Web of Science

  79. The Synthesis of Sialo-containing Glycopolymers by pi-Allyl Nickel Catalyzed Coordination Polymerization

    Ohira Shuichi, Yasuda Yu, Sato Chihiro, KItajima Ken, Tomita Ikuyoshi, Takahashi Takashi, Tanaka Hiroshi

    GLYCOBIOLOGY   Vol. 24 ( 11 ) page: 1119-1120   2014.11

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    Web of Science

  80. Interaction of 70-kDa heat shock protein with glycosaminoglycans and acidic glycopolymers Reviewed

    Yoichiro Harada, Estelle Garenaux, Takehiro Nagatsuka, Hirotaka Uzawa, Yoshihiro Nishida, Chihiro Sato, Ken Kitajima

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   Vol. 453 ( 2 ) page: 229 - 34   2014.10

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:ACADEMIC PRESS INC ELSEVIER SCIENCE  

    Interaction of Hsp70 with natural and artificial acidic glycans is demonstrated based on the native PAGE analysis. Hsp70 interacts with acidic glycopolymers that contain clustered sulfated and di-sialylated glycan moieties on a polyacrylamide backbone, but not with neutral or mono-sialylated glycopolymers. Hsp70 also interacts and forms a large complex with heparin, heparan sulfate, and dermatan sulfate that commonly contain 2-O-sulfated iduronic acid residues, but not with other types of glycosaminoglycans (GAGs). Hsp70 consists of the N-terminal ATPase domain and the C-terminal peptide-binding domain. The interaction analyses using the recombinant N- and C-terminal half domains show that the ATPase domain mediates the direct interaction with acidic glycans, while the peptide-binding domain stabilizes the large complexes with particular GAGs. To our knowledge, this is the first demonstration of direct binding of Hsp70 to the particular GAGs. This property may be involved in the physiological functions of Hsp70 at the plasma membrane and extracellular environments. (C) 2014 Elsevier Inc. All rights reserved

    DOI: 10.1016/j.bbrc.2014.05.137

    Web of Science

    Scopus

    PubMed

  81. ST8 alpha-N-acetyl-neuraminide alpha- 2,8-sialyltransferase 2 (ST8SIA2)

    Sato C.

    Handbook of Glycosyltransferases and Related Genes, Second Edition   Vol. 2   page: 781-795   2014.1

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1007/978-4-431-54240-7_77

    Scopus

  82. Polysialic acid: Biosynthesis, novel functions and applications. Reviewed

    Colley K., Kitajima K, Sato C.

    Critical Reviews in Biochemistry and Molecular Biology     page: 1-35   2014

     More details

    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

  83. Tissue-specific post-translational modification allows functional targeting of thyrotropin. Reviewed

    Ikegami K, Liao X-H, Hoshino Y, Ono H, Ota W, Ito Y, Nishiwaki-Ohkawa T, Sato C, Kitajima K, Iigo M, Shigeyoshi Y, Yamada M, Murata Y, Refetoff S, Yoshimura T.

    Cell Reports   Vol. 9   page: 1-9   2014

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  84. Comparison of analytical methods to detect polysialic acid. Reviewed

    Nishimura S, Hane M, Niimi Y, Miyata S, Kitajima K, and Sato C.

    Journal of Glycomics and Lipidomics   Vol. 4   page: 113   2014

     More details

    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

  85. Interaction of 70-kDa heat shock protein with glycosaminoglycans and acidc glycopolymers. Reviewed

    Harada Y, Garenaux E, Nagatsuka T, Uzawa H, Nishida Y, Sato C, and Kitajima K.

    Biochem Biophys Res Commun.   Vol. 453   page: 229-234   2014

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  86. Frontal affinity chromatography: Practice of weak interaction analysis between lectins and fluorescently labeled oligosaccharides Reviewed

    Chihiro Sato

    Methods in Molecular Biology   Vol. 1200   page: 257 - 64   2014

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Humana Press Inc.  

    Frontal affinity chromatography (FAC) is a simple and effective method that is applicable to the analysis of interactions between glycans and glycan-recognition proteins, including lectins, with weak affinity ranging from 10&lt
    sup&gt
    -4&lt
    /sup&gt
    to 10&lt
    sup&gt
    -6&lt
    /sup&gt
    (M) in terms of dissociation constant (K &lt
    inf&gt
    d&lt
    /inf&gt
    ). Using conventional instruments, such as a high-performance liquid chromatography (HPLC) system equipped with pump, injector, (fluorescent) detector, and data recorder, the dissociation constants for weak glycan-based interactions can be easily determined with high throughput and accuracy. Notably, if the glycans are labeled with fluorescent dyes, only a small amount of glycans is required for the analysis. Fluorescent labeling of glycans is a common technique, and an increasing number of fluorescent-labeled glycans are commercially available. In this chapter, an advanced FAC method using fluorescent-labeled glycans is described. © 2014 Springer Science+Business Media New York.

    DOI: 10.1007/978-1-4939-1292-6_22

    Web of Science

    Scopus

    PubMed

  87. Polysialic acid: Biosynthesis, novel functions and applications

    Colley Karen J., Kitajima Ken, Sato Chihiro

    CRITICAL REVIEWS IN BIOCHEMISTRY AND MOLECULAR BIOLOGY   Vol. 49 ( 6 ) page: 498-532   2014

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.3109/10409238.2014.976606

    Web of Science

    Scopus

    PubMed

  88. Comparison of analytical methods to detect polysialic acid. Reviewed

    Nishimura S, Hane M, Niimi Y, Miyata S, Kitajima K, Sato C

    Journal of Glycomics and Lipidomics   Vol. 4   page: 113   2014

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  89. Polysialic acid: Biosynthesis, novel functions and applications. Reviewed

    Colley K, Kitajima K, Sato C

    Critical Reviews in Biochemistry and Molecular Biology     page: 1-35   2014

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  90. Crystal Structure of Anti-polysialic Acid Antibody Single Chain Fv Fragment Complexed with Octasialic Acid INSIGHT INTO THE BINDING PREFERENCE FOR POLYSIALIC ACID

    Nagae Masamichi, Ikeda Akemi, Hane Masaya, Hanashima Shinya, Kitajima Ken, Sato Chihiro, Yamaguchi Yoshiki

    JOURNAL OF BIOLOGICAL CHEMISTRY   Vol. 288 ( 47 ) page: 33784 - 33796   2013.11

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1074/jbc.M113.496224

    Web of Science

    Scopus

    PubMed

  91. NMR study into the mechanism of recognition of the degree of polymerization by oligo/polysialic acid antibodies. Reviewed

    Hanashima S, Sato C, Tanaka H, Takahashi T, Kitajima K, Yamaguchi Y.

    Bioorg. Med. Chem.   Vol. 21   page: 6069-76   2013.10

     More details

    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

  92. NMR study into the mechanism of recognition of the degree of polymerization by oligo/polysialic acid antibodies Reviewed

    Shinya Hanashima, Chihiro Sato, Hiroshi Tanaka, Takashi Takahashi, Ken Kitajima, Yoshiki Yamaguchi

    BIOORGANIC & MEDICINAL CHEMISTRY   Vol. 21 ( 19 ) page: 6069 - 76   2013.10

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:PERGAMON-ELSEVIER SCIENCE LTD  

    Oligo/polysialic acids consisting of consecutive alpha(2,8)-linkages on gangliosides and glycoproteins play a role in cell adhesion and differentiation events in a manner that is dependent on the degree of polymerization (DP). Anti-oligo/polysialic acid antibodies often have DP-dependent antigenic specificity, and such unique antibodies are often used in biological studies for the detection and differentiation of oligo/polysialic acids. However, molecular mechanisms remain unclear. We here use NMR techniques to analyze the binding epitopes of the anti-oligo/polysialic acid monoclonal antibodies (mAb) A2B5 and 12E3. The mAb A2B5, which has a preference for trisialic acid, recognizes sialic acid residues at the non-reducing terminus and those in nascent units. On the other hand, mAb 12E3, which prefers oligo/polysialic acids of more than six sugar units, recognizes inner sialic acid residues. In both structural complexes, the interresidue transferred NOE correlations are significantly different from those arising from analogs of the free states, indicating that the bound and free sugar conformations are distinct. The ability of the two mAbs to distinguish the chain lengths comes from different binding epitopes and possibly from the conformational differences in the oligo/polysialic acids. Information on the recognition modes is needed for the structural design of immunoreactive antigens for the development of high-affinity anti-polysialic acid antibodies and of related vaccines against pathogenic, polysialic acid-coated bacteria. (c) 2013 Elsevier Ltd. All rights reserved.

    DOI: 10.1016/j.bmc.2013.07.023

    Web of Science

    Scopus

    PubMed

  93. Polysialic acid

    Sato C.

    Sialobiology: Structure, Biosynthesis and Function. Sialic Acid Glycoconjugates in Health and Disease     page: 33-75   2013.8

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.2174/9781608053865113010005

    Scopus

  94. Disialic, oligosialic and polysialic acids: distribution, functions and related disease Reviewed

    Chihiro Sato, Ken Kitajima

    JOURNAL OF BIOCHEMISTRY   Vol. 154 ( 2 ) page: 115 - 36   2013.8

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:OXFORD UNIV PRESS  

    Sialic acids (Sia) are involved in many biological activities and frequently exist as monosialyl residues at the non-reducing terminal end of glycoconjugates. Occasionally, polymerized structures in the form of disialic acid (diSia), oligosialic acid (oligoSia) and polysialic acid (polySia) are also found in glycoconjugates. In particular, polySia, which is an evolutionarily conserved epitope from sea urchin to humans, is one of the most biologically important glycotopes in vertebrates. The biological functions of polySia, especially on neural cell adhesion molecules, have been well studied and an in-depth body of knowledge concerning polySia has been accumulated. However, considerably less attention has been paid to glycoproteins containing di- and oligoSia groups. However, advances in analytical methods for detecting oligo/polymerized structures have allowed the identification and characterization of an increasing number of glycoproteins containing di/oligo/polySia chains in nature. In addition, sophisticated genetic techniques have also helped to elucidate the underlying mechanisms of polySia-mediated activities. In this review, recent advances in the study of the chemical properties, distribution and functions of di-, oligo- and polySia residues on glycoproteins are described.

    DOI: 10.1093/jb/mvt057

    Web of Science

    Scopus

    PubMed

  95. Occurrence of free deaminoneuraminic acid (KDN)-containing complex-type N-glycans in human prostate cancers

    Yabu Masahiko, Korekane Hiroaki, Hatano Koji, Kaneda Yasufumi, Nonomura Norio, Sato Chihiro, Kitajima Ken, Miyamoto Yasuhide

    GLYCOBIOLOGY   Vol. 23 ( 6 ) page: 634-642   2013.6

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1093/glycob/cws132

    Web of Science

    Scopus

    PubMed

  96. Impact of structural aberrancy of polysialic acid and its synthetic enzyme ST8SIA2 in schizophrenia

    Sato Chihiro, Kitajima Ken

    FRONTIERS IN CELLULAR NEUROSCIENCE   Vol. 7 ( APR ) page: 61   2013.5

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.3389/fncel.2013.00061

    Web of Science

    Scopus

    PubMed

  97. Occurrence of free deaminoneuraminic acid (KDN)-containing complex-type N-glycans in human prostate cancers. Reviewed

    Yabu M, Korekane H, Takahashi H, Ohigashi H, Ishikawa O, Hatano K, Nonomura N, Sato C, Kitajima K, Miyamoto Y.

    Glycobiology   Vol. 23   page: 634-642   2013

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  98. ポリシアル酸による神経機能の調節. Invited Reviewed

    佐藤ちひろ、北島健

    実験医学   Vol. 31   page: 26--33   2013

     More details

    Authorship:Lead author   Language:Japanese   Publishing type:Research paper (scientific journal)  

  99. Disialic, oligosialic, and polysialic acids: Distribution, biological functions, and related disease. Invited Reviewed

    Sato C., Kitajima K.

    J Biochem (Tokyo).   Vol. 154   page: 115-136   2013

     More details

    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

  100. Impact of structural aberrancy of polysialic acid and its synthetic enzyme ST8SIA2 in schizophrenia. Reviewed

    Sato C, Kitajima K.

    Front. Cell. Neurosci.   Vol. 7 ( 61 ) page: 1-11   2013

     More details

    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

  101. Crystal structure of anti-polysialic acid antibody single chain Fv fragment complexed with octasialic acid: insight into the binding preference for polysialic acid. Reviewed

    Nagae M, Ikeda A, Hane M, Hanashima S, Kitajima K, Sato C, Yamaguchi Y.

    J Biol Chem.   Vol. 288   page: 33784-96   2013

     More details

    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

  102. Polysialic Acid

    Sato Chihiro

    SIALOBIOLOGY: BIOSYNTHESIS, STRUCTURE AND FUNCTION: SIALIC ACID GLYCOCONJUGATES IN HEALTH AND DISEASE     page: 33-75   2013

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    Web of Science

  103. ポリシアル酸による神経機能の調節. Invited Reviewed

    佐藤ちひろ, 北島健

    実験医学   Vol. 31   page: 26--33   2013

     More details

    Language:Japanese   Publishing type:Research paper (scientific journal)  

  104. Impact of structural aberrancy of polysialic acid and its synthetic enzyme ST8SIA2 in schizophrenia. Reviewed

    Sato C, Kitajima K

    Front. Cell. Neurosci.   Vol. 7 ( 61 ) page: 1-11   2013

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  105. Crystal structure of anti-polysialic acid antibody single chain Fv fragment complexed with octasialic acid: insight into the binding preference for polysialic acid. Reviewed

    Nagae M, Ikeda A, Hane M, Hanashima S, Kitajima K, Sato C, Yamaguchi Y

    J Biol Chem.   Vol. 288   page: 33784-96   2013

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  106. Occurrence of free deaminoneuraminic acid (KDN)-containing complex-type N-glycans in human prostate cancers. Reviewed

    Yabu M, Korekane H, Takahashi H, Ohigashi H, Ishikawa O, Hatano K, Nonomura N, Sato C, Kitajima K, Miyamoto Y

    Glycobiology   Vol. 23   page: 634-642   2013

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  107. Pig sperm membrane microdomains contain a highly glycosylated 15-25-kDa wheat germ agglutinin-binding protein Reviewed

    Waraporn Kasekarn, Takeru Kanazawa, Kazuki Hori, Tomoyuki Tsuchiyama, Xue Lian, Estelle Garenaux, Kessiri Kongmanas, Nongnuj Tanphaichitr, Hiroshi Yasue, Chihiro Sato, Ken Kitajima

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   Vol. 426 ( 3 ) page: 356 - 62   2012.9

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:ACADEMIC PRESS INC ELSEVIER SCIENCE  

    A highly glycosylated protein, which has unique, novel features in localization, structure, and potential function, is found in pig sperm, and named WGA-gp due to its high binding property with wheat germ agglutinin (WGA). WGA-gp is localized mainly in flagella and enriched in membrane microdomains or lipid rafts. It is not detected by ordinary protein staining methods due to a high content of both Nand O-glycans consisting of neutral monosaccharides. Interestingly. WGA-gp may be involved in intracellular Ca2+ regulation. Treatment of sperm with anti-WGA-gp antibody enhances the amplitude of Ca2+ oscillation without changing the basal intracellular Ca2+ concentrations. All these features of WGA-gp, except for different carbohydrate structures occupying most part of the molecules, are similar to those of flagellasialin in sea urchin sperm, which regulates the intracellular Ca2+ concentration. Presence of carbohydrate-enriched flagellar proteins involved in intracellular Ca2+ regulation may be a common feature among animal sperm. (c) 2012 Elsevier Inc. All rights reserved.

    DOI: 10.1016/j.bbrc.2012.08.090

    Web of Science

    Scopus

    PubMed

  108. Metabolism of Vertebrate Amino Sugars with N-Glycolyl Groups RESISTANCE OF alpha 2-8-LINKED N-GLYCOLYLNEURAMINIC ACID TO ENZYMATIC CLEAVAGE Reviewed

    Leela R. L. Davies, Oliver M. T. Pearce, Matthew B. Tessier, Siavash Assar, Victoria Smutova, Maria Pajunen, Mizuki Sumida, Chihiro Sato, Ken Kitajima, Jukka Finne, Pascal Gagneux, Alexey Pshezhetsky, Robert Woods, Ajit Varki

    JOURNAL OF BIOLOGICAL CHEMISTRY   Vol. 287 ( 34 ) page: 28917 - 31   2012.8

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC  

    The sialic acid (Sia) N-acetylneuraminic acid (Neu5Ac) and its hydroxylated derivative N-glycolylneuraminic acid (Neu5Gc) differ by one oxygen atom. CMP-Neu5Gc is synthesized from CMP-Neu5Ac, with Neu5Gc representing a highly variable fraction of total Sias in various tissues and among different species. The exception may be the brain, where Neu5Ac is abundant and Neu5Gc is reported to be rare. Here, we confirm this unusual pattern and its evolutionary conservation in additional samples from various species, concluding that brain Neu5Gc expression has been maintained at extremely low levels over hundreds of millions of years of vertebrate evolution. Most explanations for this pattern do not require maintaining neural Neu5Gc at such low levels. We hypothesized that resistance of alpha 2-8-linked Neu5Gc to vertebrate sialidases is the detrimental effect requiring the relative absence of Neu5Gc from brain. This linkage is prominent in polysialic acid (polySia), a molecule with critical roles in vertebrate neural development. We show that Neu5Gc is incorporated into neural polySia and does not cause in vitro toxicity. Synthetic polymers of Neu5Ac and Neu5Gc showed that mammalian and bacterial sialidases are much less able to hydrolyze alpha 2-8-linked Neu5Gc at the nonreducing terminus. Notably, this difference was not seen with acid-catalyzed hydrolysis of polySias. Molecular dynamics modeling indicates that differences in the three-dimensional conformation of terminal saccharides may partly explain reduced enzymatic activity. In keeping with this, polymers of N-propionylneuraminic acid are sensitive to sialidases. Resistance of Neu5Gc-containing polySia to sialidases provides a potential explanation for the rarity of Neu5Gc in the vertebrate brain.

    DOI: 10.1074/jbc.M112.365056

    Web of Science

    Scopus

    PubMed

  109. Structural and functional impairments of polySia-NCAM synthesized by a mutated polysialyltransferase of a schizophrenic patient. Reviewed

    Hane M, Sumida M, Kitajima K, Sato C.

    Pure Appl Chem   Vol. 84   page: 1895-1906   2012.6

     More details

    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

  110. Structural and functional impairments of polySia-NCAM synthesized by a mutated polysialyltransferase of a schizophrenic patient. Reviewed

    Hane M, Sumida M, Kitajima K, Sato C

    Pure Appl Chem   Vol. 84   page: 1895-1906   2012.6

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  111. Sialidase NEU4 Hydrolyzes Polysialic Acids of Neural Cell Adhesion Molecules and Negatively Regulates Neurite Formation by Hippocampal Neurons Reviewed

    Kohta Takahashi, Junya Mitoma, Masahiro Hosono, Kazuhiro Shiozaki, Chihiro Sato, Kazunori Yamaguchi, Ken Kitajima, Hideyoshi Higashi, Kazuo Nitta, Hiroshi Shima, Taeko Miyagi

    JOURNAL OF BIOLOGICAL CHEMISTRY   Vol. 287 ( 18 ) page: 14816 - 26   2012.4

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC  

    Modulation of levels of polysialic acid (polySia), a sialic acid polymer, predominantly associated with the neural cell adhesion molecule (NCAM), influences neural functions, including synaptic plasticity, neurite growth, and cell migration. Biosynthesis of polySia depends on two polysialyltransferases ST8SiaII and ST8SiaIV in vertebrate. However, the enzyme involved in degradation of polySia in its physiological turnover remains uncertain. In the present study, we identified and characterized a murine sialidase NEU4 that catalytically degrades polySia. Murine NEU4, dominantly expressed in the brain, was found to efficiently hydrolyze oligoSia and polySia chains as substrates in sialidase in vitro assays, and also NCAM-Fc chimera as well as endogenous NCAM in tissue homogenates of postnatal mouse brain as assessed by immunoblotting with anti-polySia antibodies. Degradation of polySia by NEU4 was also evident in neuroblastoma Neuro2a cells that were co-transfected with Neu4 and ST8SiaIV genes. Furthermore, in mouse embryonic hippocampal primary neurons, the endogenously expressed NEU4 was found to decrease during the neuronal differentiation. Interestingly, GFP- or FLAG-tagged NEU4 was partially co-localized with polySia in neurites and significantly suppressed their outgrowth, whereas silencing of NEU4 showed the acceleration together with an increase in polySia expression. These results suggest that NEU4 is involved in regulation of neuronal function by polySia degradation in mammals.

    DOI: 10.1074/jbc.M111.324186

    Web of Science

    Scopus

    PubMed

  112. Sialome analysis of the cephalochordate Branchiostoma belcheri, a key organism for vertebrate evolution

    Guerardel Yann, Chang Lan-Yi, Fujita Akiko, Coddeville Bernadette, Maes Emmanuel, Sato Chihiro, Harduin-Lepers Anne, Kubokawa Kaoru, Kitajima Ken

    GLYCOBIOLOGY   Vol. 22 ( 4 ) page: 479-491   2012.4

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1093/glycob/cwr155

    Web of Science

    Scopus

    PubMed

  113. Sialidase NEU4 hydrolyzes polysialic acids of neural cell adhesion molecules and negatively regulates neurite formation by hippocampal neurons. Reviewed

    Takahashi K, Mitoma J, Hosono M, Shiozaki K, Sato C, Yamaguchi K, Kitajima K, Higashi H, Nitta K, Shima H, Miyagi T.

    J Biol Chem.   Vol. 287   page: 14816-14826   2012.3

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  114. Novel Regulation of Fibroblast Growth Factor 2 (FGF2)-mediated Cell Growth by Polysialic Acid Reviewed

    Sayaka Ono, Masaya Hane, Ken Kitajima, Chihiro Sato

    JOURNAL OF BIOLOGICAL CHEMISTRY   Vol. 287 ( 6 ) page: 3710 - 22   2012.2

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC  

    Polysialic acid (polySia) is a unique polysaccharide that modifies neural cell adhesion molecule (NCAM) spatiotemporally. Recently, we demonstrated that polySia functions as a reservoir for several neurotrophic factors and neurotransmitters. Here, we showed the direct interaction between polySia and fibroblast growth factor-2 (FGF2) by native-PAGE, gel filtration, and surface plasmon resonance. The minimum chain length of polySia required for the interaction with FGF2 was 17. Compared with heparan sulfate, a well known glycosaminoglycan capable of forming a complex with FGF2, polySia formed a larger complex with distinct properties in facilitating oligomerization of FGF2, as well as in binding to FGF receptors. In polySia-NCAM-expressing NIH-3T3 cells, which were established by transfecting cells with either of the plasmids for the expression of the polysialyltransferases ST8SiaII/STX and ST8SiaIV/PST that can polysialylate NCAM, FGF2-stimulated cell growth, but not cell survival, was inhibited. Taken together, these results suggest that polySia-NCAM might be involved in the regulation of FGF2-FGF receptor signaling through the direct binding of FGF2 in a manner distinct from heparan sulfate.

    DOI: 10.1074/jbc.M111.276618

    Web of Science

    Scopus

    PubMed

  115. Novel regulation of FGF2-mediated cell growth by polysialic acid. Reviewed

    Ono S, Hane M, Kitajima K, Sato C.

    J Biol Chem.     page: 3710-22.   2012

     More details

    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

  116. Sialome analysis of the cephalochordate Branchiostoma belcheri, a key organism for vertebrate evolution. Reviewed

    Guérardel Y, Chang LY, Fujita A, Coddeville B, Maes E, Sato C, Harduin-Lepers A, Kubokawa K, Kitajima K.

    Glycobiolgy     page: in press   2012

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  117. 26 Combinatorial Synthesis of Ganglio-Series Ganglioside Epitopes and Their Biological Evaluation(Oral Presentation)

    Tanaka Hiroshi, Nishiura Yuji, Yasuda Yu, Sato Chihiro, Kitajima Ken, Takahashi Takashi

    Symposium on the Chemistry of Natural Products, symposium papers   Vol. 54 ( 0 ) page: 151-156   2012

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    Gangliosides are a family of glycolipids, which possess sialic acids that play important roles in biological events on cell surfaces. Ganglio-series gangliosides are composed of the tetrasaccharide, (Galβ(1,3)GalNAcβ(1,4)Galβ(1,4)Glc), which bears α(2,8) oligosialic acid units. Glycolipids that vary in number of sialic acids are known as the a-, b-, and c-series of gangliosides. Chemical synthesis of these di-/oligo-sialo-containing glycolipids and their derivatives would facilitate identification of their biological roles. However, the α(2,8) oligosialic acid unit and the compact and rigid 3,4 dibranched galactoside unit are difficult to prepare. Therefore, an effective method for the synthesis of a-, b-, and c-series of gangliosides is still needed. In this report, we describe combinatorial synthesis of ganglio-series ganglioside epitopes varying at the number of the sialic acids. The α(2,3) and α(2,8) sialylations were accomplished by use of 5N,4O-carbonyl and 7,8-O-isopropyliden, and 5N,4O-carbonyl- and 7,8-di-O-chloroacetyl-protected sialyl donors in good yields with excellent a-selectivity, respectively. The two sialyl donors enable synthesis of the di- and tri-sialylgalactosides by simple glycosylation and deprotection. We successfully prepared the ganglioside epitopes via direct construction of a compact, rigid, branched structure. We also reported on a competitive inhibiting activity of the soluble glycolipid library to binding solid-supported GD3 with Siglec-7.

    DOI: 10.24496/tennenyuki.54.0_151

    CiNii Article

  118. Structural and functional impairments of polysialic acid (polySia)-neural cell adhesion molecule (NCAM) synthesized by a mutated polysialyltransferase of a schizophrenic patient

    Hane Masaya, Sumida Mizuki, Kitajima Ken, Sato Chihiro

    PURE AND APPLIED CHEMISTRY   Vol. 84 ( 9 ) page: 1895-1906   2012

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1351/PAC-CON-11-12-10

    Web of Science

    Scopus

  119. Sialome analysis of the cephalochordate Branchiostoma belcheri, a key organism for vertebrate evolution. Reviewed

    Guérardel Y, Chang LY, Fujita A, Coddeville B, Maes E, Sato C, Harduin-Lepers A, Kubokawa K, Kitajima K

    Glycobiolgy     page: in press   2012

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  120. Co-expression of two distinct polysialic acids, α2,8- and α2,9-linked polymers of N-acetylneuraminic acid, in distinct glycoproteins and glycolipids in sea urchin sperm. Reviewed

      Vol. 21   page: 1596-1605   2011.12

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  121. Co-expression of two distinct polysialic acids, alpha 2,8-and alpha 2,9-linked polymers of N-acetylneuraminic acid, in distinct glycoproteins and glycolipids in sea urchin sperm

    Miyata Shinji, Yamakawa Nao, Toriyama Masaru, Sato Chihiro, Kitajima Ken

    GLYCOBIOLOGY   Vol. 21 ( 12 ) page: 1596-1605   2011.12

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1093/glycob/cwr081

    Web of Science

    Scopus

    PubMed

  122. New function of polysialic acid and its involvement in neural activities

    Sato C.

    Seikagaku   Vol. 83 ( 3 ) page: 189-196   2011.12

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    Scopus

  123. Co-expression of two distinct polysialic acids, α2,8- and α2,9-linked polymers of N-acetylneuraminic acid, in distinct glycoproteins and glycolipids in sea urchin sperm. Reviewed

      Vol. 21   page: 1596-1605   2011.12

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  124. New Functions of Polysialic Acid and Its Relationship to Schizophrenia

    SATO Chihiro, KITAJIMA Ken

    Trends in Glycoscience and Glycotechnology   Vol. 23 ( 133 ) page: 221-238   2011.9

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    Polysialic acid (polySia), particularly &alpha;2,8-linked polyNeu5Ac (DP=8&sim;400), is a unique polymer that spatio-temporally modifies neural cell adhesion molecule (NCAM), predominantly in embryonic brains. PolySia is considered to function as a negative regulator of adhesion between cells, and between cells and extracellular matrix due to its extremely polyanionic nature and steric hindrance. Through its anti-adhesive property and spatio-temporal expression, polySia is involved in cell migration, neural outgrowth, axonal guidance, synaptic plasticity, and the development of normal neural circuits and neurogenesis. Due to improvements of polySia-detection methods, it has been shown that polySia expression persists in the hippocampus and olfactory bulb of adults, where neurogenesis is ongoing; however, the functions of polySia, particularly in adult brains, remain incompletely resolved. Recently, we demonstrated that polySia functions not only as an anti-adhesive molecule, but also as a reservoir for specific bioactive molecules, such as neurotrophic factors, neurotransmitters, and growth factors, which regulate neural function. In addition, studies have revealed that the enzymatic activity of STX, which is involved in the synthesis of polySia, derived from schizophrenic patient is low and that the product of the enzyme, polySia on NCAM is impaired with respect to both quantity and quality. Notably, impaired polySia-NCAM cannot effectively retain bioactive molecules, including BDNF, dopamine, and FGF2. Taken together, the impairment of polySia might influence the retention function of polySia and lead to the development of psychiatric disorders, such as schizophrenia, which are reported to express either lower or elevated amounts of polySia compared with normal brains.

    DOI: 10.4052/tigg.23.221

    Web of Science

    Scopus

    CiNii Article

  125. The Roles of Carbohydrate Binding in Cell Adhesion and Inflammation Reviewed

    Ken Kitajima, Chihiro Sato

    Carbohydrate Recognition: Biological Problems, Methods, and Applications     page: 33 - 63   2011.7

     More details

    Language:English   Publishing type:Part of collection (book)   Publisher:John Wiley and Sons  

    DOI: 10.1002/9781118017586.ch2

    Scopus

  126. Structural and Functional Impairments of Polysialic Acid by a Mutated Polysialyltransferase Found in Schizophrenia

    Isomura Ryo, Kitajima Ken, Sato Chihiro

    JOURNAL OF BIOLOGICAL CHEMISTRY   Vol. 286 ( 24 ) page: 21535-21545   2011.6

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1074/jbc.M111.221143

    Web of Science

    Scopus

    PubMed

  127. New function of polysialic acid and its involvement in neural activities

    SATO Chihiro, KITAJIMA Ken

      Vol. 83 ( 3 ) page: 189-196   2011.3

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    CiNii Article

  128. ポリシアル酸の新機能と神経形成維持機構 Reviewed

    佐藤ちひろ, 北島 健

    生化学   Vol. 83   page: 189-196   2011.3

     More details

    Authorship:Lead author   Language:Japanese   Publishing type:Research paper (scientific journal)  

  129. [New function of polysialic acid and its involvement in neural activities].

    Sato C, Kitajima K

    Seikagaku. The Journal of Japanese Biochemical Society   Vol. 83 ( 3 ) page: 189-96   2011.3

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    PubMed

  130. New functions of polysialic acid and its relationship to schizophrenia Reviewed

    Sato C and Kitajima K

      Vol. 23   page: 221-238   2011

     More details

    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

  131. Structural and functional impairments of polysialic acid by a mutated polysialyltransferase found in schizophrenia Reviewed

    Isomura, R., Kitajima, K., and Sato, C.

    J. Biol. Chem.   Vol. 286   page: 21535-21545   2011

     More details

    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

  132. Human KDN (deaminated neuraminic acid) and its elevated expression in cancer cells: Mechanism and significance Reviewed

    Sadako Inoue, Ken Kitajima, Chihiro Sato, Shinji Go

    Advances in Experimental Medicine and Biology   Vol. 705   page: 669 - 78   2011

     More details

    Language:English   Publishing type:Research paper (international conference proceedings)  

    Sialic acids are a family of nine-carbon carboxylated sugars having a nonulosonate skeletal structure (Fig. 35.1). This structure is uniquely different from that of other sugar units of animal glycans. The most popular sialic acid is N-acetylneuraminic acid (Neu5Ac), which is universally found on cell surface glycocalyx and in secreted glycoproteins of vertebrates and some invertebrates. Sialic acids have low acid-base dissociation constants and give a negative charge on the cell surface under a wide range of physiological pH. In nature, more than 50 kinds of sialic acids are known. Nearly all of them are derived from Neu5Ac by a substitution on the hydroxyl groups (e.g., O-acetyl-Neu5Ac) and/or a hydroxylation of the N-acetyl group (e.g., N-glycolylneuraminic acid, Neu5Gc). Each modified sialic acid has properties different from those of Neu5Ac and is believed to contribute to specific physiological functions. In animal cells, sialic acids are most frequently the terminal sugars of cell surface glycolipids and glycoproteins, and any change that occurs on sialic acids can considerably influence the biological properties of a cell. © 2011 Springer Science+Business Media, LLC.

    DOI: 10.1007/978-1-4419-7877-6_35

    Scopus

    PubMed

  133. Mechanism Regulating Ca2+-dependent Mechanosensory Behaviour in Sea Urchin Spermatozoa Reviewed

    Yuka Kambara, Kogiku Shiba, Manabu Yoshida, Chihiro Sato, Ken Kitajima, Chikako Shingyoji

    CELL STRUCTURE AND FUNCTION   Vol. 36 ( 1 ) page: 69 - 82   2011

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:JAPAN SOC CELL BIOLOGY  

    Flagellar movement of the sea urchin sperm is regulated by intracellular Ca2+. Flagellasialin, a polysialic acid-containing glycoprotein, as well as other membrane proteins seems responsible for the Ca2+ control. To elucidate the mechanism of Ca2+ dynamics underlying flagellar movement, we analysed the sperm's mechanosensory behavioural responses by using microtechniques. In sea water containing 10 mM Ca2+, the sperm swim in circular paths. When a mechanical stimulus was applied to the sperm head with a glass microstylus, the sperm showed a series of flagellar responses, consisting of a stoppage of beating (quiescence) and a recovery of swimming in a straight path, followed by swimming in a circular path again; as the result the sperm avoided the obstacle. Ca2+-imaging with Fluo-4 showed that the intracellular Ca2+ was high in the quiescence and gradually decreased after that. The effects of blockers and antibodies against candidate components revealed that the Ca2+ influx was induced by Ca2+ channels and the Ca2+ efflux was induced by a flagellasialin-related Ca2+-efflux system, plasma membrane Ca2+-ATPases and the K+-dependent Na+/Ca2+ exchanger. The results show that the Ca2+-dependent mechanosensory behaviour of the sea urchin sperm is regulated by organized functioning of the membrane environment including the plasma membrane proteins and flagellasialin.

    DOI: 10.1247/csf.10020

    Web of Science

    Scopus

    PubMed

    CiNii Article

  134. Mechanism Regulating Ca2+-dependent Mechanosensory Behaviour in Sea Urchin Spermatozoa

    Kambara Yuka, Shiba Kogiku, Yoshida Manabu, Sato Chihiro, Kitajima Ken, Shingyoji Chikako

    CELL STRUCTURE AND FUNCTION   Vol. 36 ( 1 ) page: 69-82   2011

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    Web of Science

  135. Structural and functional impairments of polysialic acid by a mutated polysialyltransferase found in schizophrenia Reviewed

    Isomura, R, Kitajima, K, Sato, C

    J. Biol. Chem.   Vol. 286   page: 21535-21545   2011

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  136. New functions of polysialic acid and its relationship to schizophrenia Reviewed

    Sato C, Kitajima K

      Vol. 23   page: 221-238   2011

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  137. IgG2 dominancy and carbohydrate recognition specificity of C3H/He mouse antibodies directed to cross-reactive carbohydrate determinants (CCDs) bearing beta-(1,2)-xylose and alpha-(1,3)-fucose

    Hino Shingo, Umeda Fumiko, Inumaru Shigeki, Aoki Naohito, Sato Chihiro, Okajima Tetsuya, Nadano Daita, Matsuda Tsukasa

    IMMUNOLOGY LETTERS   Vol. 133 ( 1 ) page: 28-34   2010.9

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.imlet.2010.06.007

    Web of Science

    Scopus

    PubMed

  138. Developmental stage-dependent expression of an alpha 2,8-trisialic acid unit on glycoproteins in mouse brain Reviewed

    Emi Inoko, Yuji Nishiura, Hiroshi Tanaka, Takashi Takahashi, Koichi Furukawa, Ken Kitajima, Chihiro Sato

    GLYCOBIOLOGY   Vol. 20 ( 7 ) page: 916 - 28   2010.7

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:OXFORD UNIV PRESS INC  

    The monoclonal antibody mAb.A2B5 is a marker for the detection of oligodendrocyte progenitor cells that differentiate into type-2 astrocytes and oligodendrocytes. It is also a useful antibody for separating these cells from other lineage populations. The epitope of this antibody is considered to be the gangliosides GT3 and GQ1c. In this study, we sought to define more precisely the structure of the epitope. Accordingly, we chemically synthesized defined oligosialic acid structures linked to phosphatidylethanolamine and bovine serum albumin and used these to determine the antigenic specificity. mAb.A2B5 recognized the Neu5Ac alpha 2 -&gt; 8Neu5Ac alpha 2 -&gt; 8Neu5Ac alpha -&gt; structure on both glycolipids and glycoproteins. We then examined whether the mAb.A2B5 epitope exists on glycoproteins in developing mouse brains. Western blot analyses revealed the expression of four glycoproteins reactive with the mAb.A2B5, and their expression was dependent on the stage of neural development. All the immunoreactivity in these glycoproteins with mAb.A2B5 disappeared after sialidase treatment and were resistant to chloroform/methanol extraction. These epitopes were also detected in brain homogenates from both GD3 synthetase-null and GD3/GD2 synthetase double null mice. These findings show that the alpha 2,8-trisialic acid (triSia) unit recognized by mAb.A2B5 resides not only on gangliosides but also on glycoproteins in developing mouse brain. We postulate that the triSia structure on glycoproteins may be involved in oligodendrocyte differentiation, similar to the case with the alpha 2,8-triSia structure on gangliosides. Real time polymerase chain reaction analysis of the developmental expression of all known ST8Sia genes, which are responsible for the biosynthesis of alpha 2,8-linked Sia residues, showed that ST8Sia III gene expression correlated with expression of the triSia epitope. We suggest that ST8Sia III is the principal sialyltransferase responsible for synthesis of the alpha 2,8-triSia units on glycoproteins.

    DOI: 10.1093/glycob/cwq049

    Web of Science

    Scopus

    PubMed

  139. Extensive enrichment of N-glycolylneuraminic acid in extracellular sialoglycoproteins abundantly synthesized and secreted by human cancer cells

    Inoue Sadako, Sato Chihiro, Kitajima Ken

    GLYCOBIOLOGY   Vol. 20 ( 6 ) page: 752-762   2010.6

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1093/glycob/cwq030

    Web of Science

    Scopus

    PubMed

  140. 糖鎖と統合失調症 Invited

    佐藤ちひろ

    生体の科学   Vol. 61   page: 160-166   2010

     More details

    Authorship:Lead author   Language:Japanese   Publishing type:Research paper (scientific journal)  

  141. IgG2 dominancy and carbohydrate recognition specificity of C3H/He mouse antibodies directed to cross-reactive carbohydrate determinants (CCDs) bearing beta-(1,2)-xylose and alpha-(1,3)-fucose. Reviewed

    Hino S, Umeda F, Inumaru S, Aoki N, Sato, C., Okajima T, Nadano D, Matsuda T.

    Immunol Lett   Vol. 133   page: 28-34   2010

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  142. Survival signals of hepatic stellate cells in liver regeneration are regulated by glycosylation changes in rat vitronectin, especially decreased sialylation. Reviewed

    Sano K, Miyamoto Y, Kawasaki N, Hashii N, Itoh S, Murase M, Date K, Yokoyama M, Sato, C., Kitajima K, Ogawa H.

    J Biol Chem   Vol. 285   page: 17301-17309   2010

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  143. Extensive enrichment of N-glycolylneuraminic acid in extracellular sialoglycoproteins abundantly synthesized and secreted by human cancer cells. Reviewed

    Inoue, S., Sato, C. and Kitajima, K

    Glycobiology   Vol. 20   page: 752-762   2010

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  144. Developmental stage-dependent expression of an α2,8-trisialic acid unit on glycoproteins in mouse brain. Reviewed

    Inoko, E., Nishiura, Y., Tanaka, H., Takahashi, T., Furukawa, K., Kitajima, K., and Sato, C.

    Glycobiology   Vol. 20   page: 916-928   2010

     More details

    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

  145. MEASUREMENT OF GLYCAN-BASED INTERACTIONS BY FRONTAL AFFINITY CHROMATOGRAPHY AND SURFACE PLASMON RESONANCE

    Sato Chihiro, Yamakawa Nao, Kitajima Ken

    METHODS IN ENZYMOLOGY, VOL 478: GLYCOMICS   Vol. 478 ( C ) page: 219-232   2010

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/S0076-6879(10)78010-1

    Web of Science

    Scopus

    PubMed

  146. Survival signals of hepatic stellate cells in liver regeneration are regulated by glycosylation changes in rat vitronectin, especially decreased sialylation. Reviewed

    Sano K, Miyamoto Y, Kawasaki N, Hashii N, Itoh S, Murase M, Date K, Yokoyama M, Sato, C, Kitajima K, Ogawa H

    J Biol Chem   Vol. 285   page: 17301-17309   2010

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  147. 糖鎖と統合失調症 Invited

    佐藤ちひろ

    生体の科学   Vol. 61   page: 160-166   2010

     More details

    Language:Japanese   Publishing type:Research paper (scientific journal)  

  148. IgG2 dominancy and carbohydrate recognition specificity of C3H/He mouse antibodies directed to cross-reactive carbohydrate determinants (CCDs) bearing beta-(1,2)-xylose and alpha-(1,3)-fucose. Reviewed

    Hino S, Umeda F, Inumaru S, Aoki N, Sato, C, Okajima T, Nadano D, Matsuda T

    Immunol Lett   Vol. 133   page: 28-34   2010

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  149. Extensive enrichment of N-glycolylneuraminic acid in extracellular sialoglycoproteins abundantly synthesized and secreted by human cancer cells. Reviewed

    Inoue, S, Sato, C, Kitajima, K

    Glycobiology   Vol. 20   page: 752-762   2010

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  150. Complex Formation of a Brain-Derived Neurotrophic Factor and Glycosaminoglycans

    Kanato Yukihiro, Ono Sayaka, Kitajima Ken, Sato Chihiro

    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY   Vol. 73 ( 12 ) page: 2735-2741   2009.12

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1271/bbb.90637

    Web of Science

    Scopus

    PubMed

    CiNii Article

  151. Developmental regulation of oligosialylation in zebrafish

    Chang Lan-Yi, Harduin-Lepers Anne, Kitajima Ken, Sato Chihiro, Huang Chang-Jen, Khoo Kay-Hooi, Guerardel Yann

    GLYCOCONJUGATE JOURNAL   Vol. 26 ( 3 ) page: 247-261   2009.4

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1007/s10719-008-9161-5

    Web of Science

    Scopus

    PubMed

  152. Membrane microdomains from early gastrula embryos of medaka, Oryzias latipes, are a platform of E-cadherin- and carbohydrate-mediated cell-cell interactions during epiboly

    Adachi Tomoko, Sato Chihiro, Kishi Yasunori, Totani Kazuhide, Murata Takeomi, Usui Taichi, Kitajima Ken

    GLYCOCONJUGATE JOURNAL   Vol. 26 ( 3 ) page: 285-299   2009.4

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1007/s10719-008-9184-y

    Web of Science

    Scopus

    PubMed

  153. Periodate-resistant carbohydrate epitopes recognized by IgG and IgE antibodies from some of the immunized mice and patients with allergy Reviewed

    Shingo Hino, Takeshi Matsubara, Atsuo Urisu, Naohito Aoki, Chihiro Sato, Tetsuya Okajima, Daita Nadano, Tsukasa Matsuda

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   Vol. 380 ( 3 ) page: 632 - 7   2009.3

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:ACADEMIC PRESS INC ELSEVIER SCIENCE  

    The beta(1,2)-xylose- and/or alpha(1,3)-fucose-containing cross-reactive carbohydrate determinants (CCDs) are present in various plant and insect N-glycans, and have been attracted as potential antigens in IgE-mediated allergies and immunologically undesired post-translational products on some recombinant therapeutic proteins. By using ELISA and immunoblotting, CCDs-specific IgG and IgE antibodies from some, but not all, of mice and humans were found to fully retain their binding activity after a typical periodate-treatment to CCDs, which did cause the CCDs&apos; antigenic activity to those from the other mice and rabbits to disappear almost completely. Furthermore, the mouse IgE recognizing the periodate-resistant CCDs induced the CCDs/IgE-dependent degranulation of rat basophilic RBL-2H3 cells. These findings indicate that in some cases CCDs include those dependent of the core trisaccharide more strongly than the terminal xylose and fucose, which might have been screened out in the CCDs analyses based on the loss of antibody-binding by the periodate-treatment. (C) 2009 Elsevier Inc. All rights reserved.

    DOI: 10.1016/j.bbrc.2009.01.138

    Web of Science

    Scopus

    PubMed

  154. Membrane microdomains from early gastrula embryos of medaka, Oryzias latipes are a plat form of E-cadherin and carbohydrate-mediated cell-cell interactions during epiboly Reviewed

    Adachi T., Sato, C., Kishi, Y., Totani, K., Murata, T., Usui, T., and Kitajima, K

    Glycoconjugate J.   Vol. 26   page: 285-299   2009

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  155. Complex formation of a brain-derived neurotrophic factor and glycosaminoglycans. Reviewed

    Kanato, Y., Kitajima, K. and Sato, C.

    Biosci. Biotech. Biosci.   Vol. 12   page: 1044-1053   2009

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  156. Developmental regulation of oligosialylation in zebrafish. Reviewed

    Chang LY, Harduin-Lepers A, Kitajima K, Sato C, Huang CJ, Khoo KH, Guérardel Y.

    Glycoconjugate J.   Vol. 26   page: 247-61   2009

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  157. 3P-066 シアル酸結合免疫グロブリン様レクチン(シグレック)-7によるシアロ糖鎖認識の生化学的および分子ドッキング解析(蛋白質-機能(反応機構,生物活性など),第47回日本生物物理学会年会)

    生物物理   Vol. 49 ( 0 )   2009

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.2142/biophys.49.S162_2

    CiNii Article

  158. Complex formation of a brain-derived neurotrophic factor and glycosaminoglycans. Reviewed

    Kanato, Y, Kitajima, K, Sato, C

    Biosci. Biotech. Biosci.   Vol. 12   page: 1044-1053   2009

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  159. Developmental regulation of oligosialylation in zebrafish. Reviewed

    Chang LY, Harduin-Lepers A, Kitajima K, Sato C, Huang CJ, Khoo KH, Guérardel Y

    Glycoconjugate J.   Vol. 26   page: 247-61   2009

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  160. Membrane microdomains from early gastrula embryos of medaka, Oryzias latipes are a plat form of E-cadherin and carbohydrate-mediated cell-cell interactions during epiboly Reviewed

    Adachi T, Sato, C, Kishi, Y, Totani, K, Murata, T, Usui, T, Kitajima, K

    Glycoconjugate J.   Vol. 26   page: 285-299   2009

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  161. Direct binding of polysialic acid to a brain-derived neurotrophic factor depends on the degree of polymerization

    Kanato Yukihiro, Kitajima Ken, Sato Chihiro

    GLYCOBIOLOGY   Vol. 18 ( 12 ) page: 1044-1053   2008.12

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1093/glycob/cwn084

    Web of Science

    Scopus

    PubMed

  162. [Regulation of signal transduction by polysialic acid]. Reviewed

    Sato C, Kitajima K

    Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme   Vol. 53 ( 12 Suppl ) page: 1577 - 1583   2008.9

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    PubMed

  163. Direct binding of polysialic acid to a brain-derived neurotrophic factor depends on the degree of polymerization Reviewed

    Kanato, Y, Kitajima, K., and Sato, C.

    Glycobiology   Vol. 12   page: 1044-1053   2008

     More details

    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

  164. ポリシアル酸構造による細胞シグナルの調節機構 Invited

    佐藤ちひろ、北島健

    蛋白質核酸酵素   Vol. 53 ( 1577-1583 )   2008

     More details

    Language:Japanese   Publishing type:Research paper (scientific journal)  

  165. Direct binding of polysialic acid to a brain-derived neurotrophic factor depends on the degree of polymerization Reviewed

    Kanato, Y, Kitajima, K, Sato, C

    Glycobiology   Vol. 12   page: 1044-1053   2008

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  166. Development of sensitive chemical and immunochemical methods for detecting sulfated sialic acids and their application to glycoconjugates from sea urchin sperm and eggs

    Yamakawa Nao, Sato Chihiro, Miyata Shinji, Maehashi Eri, Toriyama Masaru, Sato Noriko, Furuhata Kimio, Kitajima Ken

    BIOCHIMIE   Vol. 89 ( 11 ) page: 1396-1408   2007.11

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.biochi.2007.04.010

    Web of Science

    Scopus

    PubMed

  167. The rainbow trout CMP-sialic acid synthetase utilises a nuclear localization signal different from that identified in the mouse enzyme

    Tiralongo Joe, Fujita Akiko, Sato Chihiro, Kitajima Ken, Lehmann Friederike, Oschlies Melanie, Gerardy-Schahn Rita, Muenster-Kuehnel Anja K.

    GLYCOBIOLOGY   Vol. 17 ( 9 ) page: 945-954   2007.9

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1093/glycob/cwm064

    Web of Science

    Scopus

    PubMed

  168. Membrane microdomain formation is crucial in epiboly during gastrulation of medaka

    Adachi Tomoko, Sato Chihiro, Kitajima Ken

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   Vol. 358 ( 3 ) page: 848-853   2007.7

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.bbrc.2007.04.197

    Web of Science

    Scopus

    PubMed

  169. Hypoxia-enhanced expression of free deaminoneuraminic acid in human cancer cells

    Go Shinji, Sato Chihiro, Yin Jun, Kannagi Reiji, Kitajima Ken

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   Vol. 357 ( 2 ) page: 537-542   2007.6

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.bbrc.2007.03.181

    Web of Science

    Scopus

    PubMed

  170. Identification of the nuclear export signals that regulate the intracellular localization of the mouse CMP-sialic acid synthetase

    Fujita Akiko, Sato Chihiro, Kitajima Ken

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   Vol. 355 ( 1 ) page: 174-180   2007.3

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.bbrc.2007.01.139

    Web of Science

    Scopus

    PubMed

  171. Identification of an Inflammation-inducible Serum Protein Recognized by Anti-disialic Acid Antibodies as Carbonic Anhydrase II

    Yasukawa Zenta, Sato Chihiro, Kitajima Ken

    The journal of biochemistry   Vol. 141 ( 3 ) page: 429-441   2007.3

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    CiNii Article

  172. Glycobiology of polysialic acids on sea urchin gametes

    Miyata Shinji, Sato Chihiro, Kitajima Ken

    TRENDS IN GLYCOSCIENCE AND GLYCOTECHNOLOGY   Vol. 19 ( 106 ) page: 85-98   2007.3

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    Web of Science

  173. Identification of an inflammation-inducible serum protein recognized by anti-disialic acid antibodies as carbonic anhydrase II Reviewed

    Zenta Yasukawa, Chihiro Sato, Ken Kitajima

    JOURNAL OF BIOCHEMISTRY   Vol. 141 ( 3 ) page: 429 - 41   2007.3

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:JAPANESE BIOCHEMICAL SOC  

    Acute-phase proteins are an important marker of inflammation and sometimes have a role in the general defense response towards tissue injury. In the present study, we identified a 32-kDa protein that was immunoreactive with monoclonal antibody 2-4B (mAb.2-4B), which is specific to di/oligoNeu5Gc structures, and that behaved as an acute-phase protein following stimulation with either turpentine oil or lipopolysaccharides. The 32-kDa protein was identified as carbonic anhydrase II (CA-II), based on matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analyses of the purified protein. Mouse and human CA-II was immunoreactive and immunoprecipitated with mAb.2-4B, but contained no sialic acid. In addition to mAb.2-4B, the mAb. S2-566 an antibody specific for diNeu5Ac-containing glycans, recognized the CA-II, whereas an anti-oligo/polysialic acid antibody did not. These results indicate that a part of the CA-II protein structure mimics the disialic acid structure recognized by the monoclonal antibodies. This is the first report that CA-II circulates in the serum following inflammation.

    DOI: 10.1093/jb/mvm047

    Web of Science

    Scopus

    PubMed

  174. Glycobiology of Polysialic Acids on Sea Urchin Gametes

    MIYATA Shinji, SATO Chihiro, KITAJIMA Ken

    Trends in Glycoscience and Glycotechnology   Vol. 19 ( 106 ) page: 85-98   2007.3

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    Naturally occurring polysialic acids (polySias), a unique homopolymer of sialic acid (Sia) residues, have a large structural diversity, primarily arising from the diversities in Sia components as well as in the intersialyl linkages. The &alpha;2,8-linked polySia on the neural cell adhesion molecules (NCAM) is the most extensively studied and recognized as a regulator of cell-cell interactions during development and differentiation in vertebrate brain. However, little is known about the occurrence and functions of polySia other than the &alpha;2,8-linked polySia on NCAM. Recently, it has been shown that polySia occurs in nature more frequently than previously recognized, as analytical methods to detect polySia structures have been greatly improved. Using these methods, two different types of polySia, &alpha;2,8- and &alpha;2,9-linked polySia, have been discovered on the sea urchin sperm, although the &alpha;2,5<i>O<sub>glycolyl</sub></i>-linked polySia was already known in sea urchin egg. This is the first example of the co-localization of polySia with different intersialyl linkages in the same cell. The &alpha;2,9-linked polySia-containing glycoprotein of sea urchin sperm flagella was identified and named &ldquo;flagellasialin&rdquo;. The &alpha;2,9-linked polySia on flagellasialin is suggested as a regulator of intracellular Ca<sup>2+</sup> and sperm motility. In addition, the &alpha;2,5<i>O<sub>glycolyl</sub></i>-linked polySia has recently been shown to increase intracellular pH and potentiate acrosome reaction of sea urchin sperm. These results indicate that these polySia structures in sea urchin gametes have new functions other than the negative regulator for the NCAM-mediated cell adhesion. In this review, we summarize the recent advances in structural and functional studies of polySia in sea urchin gametes.

    DOI: 10.4052/tigg.19.85

    Scopus

    CiNii Article

  175. Complex formation of 70-kDa heat shock protein with acidic glycolipids and phospholipids

    Harada Yoichiro, Sato Chihiro, Kitajima Ken

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   Vol. 353 ( 3 ) page: 655-660   2007.2

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.bbrc.2006.12.068

    Web of Science

    Scopus

    PubMed

  176. Heterocomplex formation and cell-surface accumulation of hen's serum zona pellucida B1 (ZPB1) with ZPC expressed by a mammalian cell line (COS-7): A possible initiating step of egg-envelope matrix construction

    Okumura Hiroki, Aoki Naohito, Sato Chihiro, Nadano Daita, Matsuda Tsukasa

    BIOLOGY OF REPRODUCTION   Vol. 76 ( 1 ) page: 9-18   2007.1

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    Web of Science

  177. Heterocomplex formation and cell-surface accumulation of hen's serum zona pellucida B1 (ZPB1) with ZPC expressed by a mammalian cell line (COS-7): A possible initiating step of egg-envelope matrix construction Reviewed

    Hiroki Okumura, Naohito Aoki, Chihiro Sato, Daita Nadano, Tsukasa Matsuda

    BIOLOGY OF REPRODUCTION   Vol. 76 ( 1 ) page: 9 - 18   2007.1

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:SOC STUDY REPRODUCTION  

    The egg envelope, referred to as zona pellucida (ZP) in mammalian eggs, is a fibrous and noncollagenous extracellular matrix surrounding vertebrate eggs, and composed of three to four homologous glycoproteins with a common ZP domain. In birds, a liver-derived ZP glycoprotein (ZP1/ZPB1) is transported through the bloodstream to ovarian follicles and joins the egg-envelope matrix construction together with the other ZP glycoproteins, such as ZPC and ZPD/ZPX2, both secreted from follicular granulosa cells. We report here that, through its ZP domain, ZPB1 specifically associates with ZPC, which might lead to the construction of egg-envelope matrix. The ZPB1 in laying hen's serum specifically bound to ZPC, but not to ZPX2, separated by SDS-PAGE and blotted on a membrane. Hemagglutinin (HA)-tagged ZPC expressed in a mammalian cell line (COS-7) cells was processed and secreted as a mature-form into the culture medium. From the culture supernatant of ZPC-expressing transfectants cultured in the presence of ZPB1, both ZPB1 and ZPC were recovered as heterocomplexes by immunoprecipitation using either anti-HA or anti-ZPB1 antibody. Interestingly, a monoclonal antibody, 8E1, which immunoprecipitated free ZPB1, did not immunoprecipitate the ZPB1-ZPC heterocomplexes. An 8E1 epitope was mapped on a C-terminal region of the ZP domain in a ZPB1 molecule by identifying an 8E1-positive peptide using mass spectroscopy. Furthermore, by laser scanning confocal microscopy, ZPB1 and ZPC were observed to colocalize on the surface of ZPC-expressing transfectants cultured in the presence of ZPB1, whereas almost no ZPC was detected on the surface of the transfectants cultured in the absence of ZPB1. Taken together, these results suggest that ZPB1 transported into ovarian follicles encounters and associates with ZPC secreted from granulosa cells, resulting in the formation of heterocomplexes around an oocyte. In addition, it appears that such ZPB1-ZPC complexes accumulated on the oocyte surface act as a scaffold for subsequent matrix construction events including ZPX2 association.

    DOI: 10.1095/biolreprod.106.056267

    Web of Science

    Scopus

    PubMed

  178. Complex formation of 70-kDa heat shock protein with acidic glycolipids and phospholipids. Reviewed

    Harada Y, Sato C, Kitajima K.

    Biochem Biophys Res Commun.   Vol. 353   page: 655-660   2007

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  179. シアル酸の構造多様性とその生物学的意義. Invited

    北島 健、郷 慎司、藤田 明子、佐藤ちひろ

    細胞工学   Vol. 26   page: 629-634   2007

     More details

    Language:Japanese  

  180. Membrane microdomain formation is crucial in epiboly during gastrulation of medaka. Reviewed

    Adachi, T., Sato, C., and Kitajima, K.

    Biochem. Biophys. Res. Commun.   Vol. 355   page: 174-180   2007

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  181. Development of sensitive chemical and immunochemical methods for detecting sulfated sialic acids and their application to glycoconjugates from sea urchin sperm and eggs Reviewed

    Yamakawa, N., Sato C., Miyata, S., Maehashi, E., Toriyama, T., Sato, N., Furuhata, K., and Kitajima. K.

    Biochimie   Vol. 89   page: 1396-1408   2007

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  182. Glycobiology of polysialic acids on sea urchin gametes. Invited Reviewed

    Miyata, S., Sato, C., and Kitajima, K.

    Trends Glycosci. Glycotech.   Vol. 19   page: 83-95   2007

     More details

    Language:English  

  183. Hypoxia-enhanced expression of free deaminoneuraminic acid in human cancer cells. Reviewed

    Go, S., Sato, C., Yin, J., Kannagi, R., and Kitajima, K.

    Biochem. Biophys. Res. Commun.   Vol. 357   page: 537-542.   2007

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  184. Identification of the nuclear export signals that regulate the intracellular localization of the mouse CMP-sialic acid synthetase. Reviewed

    Fujita, A, Sato C, and Kitajima, K.

    Biochem. Biophys. Res. Commun.   Vol. 355   page: 174-180   2007

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  185. Identification of an inflammation-inducible serum protein recognized by anti-disialic acid antibodies as carbonic anhydrase II. Reviewed

    Yasukawa, Z., Sato, C., and Kitajima, K.

    J Biochem.   Vol. 141   page: 429-441   2007

     More details

    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

    Acute phase proteins are an important marker of inflammation and sometimes have a role in the general defense response towards tissue injury. In the present study, we identified a 32 kDa protein that was immunoreactive with monoclonal antibody 2-4B (mAb.2-4B), which is specific to di/oligoNeu5Gc structures, and that behaved as an acute phase protein following stimulation with either turpentine oil or lipopolysaccharides. The 32 kDa protein was identified as carbonic anhydrase II (CA-II), based on matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analyses of the purified protein. Mouse and human CA-II was immunoreactive and immunoprecipitated with mAb.2-4B, but contained no sialic acid. In addition to mAb.2-4B, the mAb. S2-566 an antibody specific for diNeu5Ac-containing glycans, recognized the CA-II, whereas an anti-oligo/polysialic acid antibody did not. These results indicate that a part of the CA-II protein structure mimics the disialic acid structure recognized by the monoclonal antibodies. This is the first report that CA-II circulates in the serum following inflammation.

  186. The rainbow trout CMP-sialic acid synthetase utilises a nuclear localization signal different from that identified in the mouse enzyme. Reviewed

    Tiralongo J, Fujita A, Sato C, Kitajima K, Lehmann F, Oschlies M, Gerardy-Schahn R, Munster-Kuhnel AK.

    Glycobiology   Vol. 17   page: 945-954   2007

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  187. The rainbow trout CMP-sialic acid synthetase utilises a nuclear localization signal different from that identified in the mouse enzyme. Reviewed

    Tiralongo J, Fujita A, Sato C, Kitajima K, Lehmann F, Oschlies M, Gerardy-Schahn R, Munster-Kuhnel AK

    Glycobiology   Vol. 17   page: 945-954   2007

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  188. シアル酸の構造多様性とその生物学的意義. Invited

    北島 健, 郷 慎司, 藤田 明子, 佐藤ちひろ

    細胞工学   Vol. 26   page: 629-634   2007

     More details

    Language:Japanese   Publishing type:Research paper (scientific journal)  

  189. Identification of the nuclear export signals that regulate the intracellular localization of the mouse CMP-sialic acid synthetase. Reviewed

    Fujita, A, Sato C, Kitajima, K

    Biochem. Biophys. Res. Commun.   Vol. 355   page: 174-180   2007

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  190. Hypoxia-enhanced expression of free deaminoneuraminic acid in human cancer cells. Reviewed

    Go, S, Sato, C, Yin, J, Kannagi, R, Kitajima, K

    Biochem. Biophys. Res. Commun.   Vol. 357   page: 537-542.   2007

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  191. Glycobiology of polysialic acids on sea urchin gametes. Invited Reviewed

    Miyata, S, Sato, C, Kitajima, K

    Trends Glycosci. Glycotech.   Vol. 19   page: 83-95   2007

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  192. Development of sensitive chemical and immunochemical methods for detecting sulfated sialic acids and their application to glycoconjugates from sea urchin sperm and eggs Reviewed

    Yamakawa, N, Sato C, Miyata, S, Maehashi, E, Toriyama, T, Sato, N, Furuhata, K, Kitajima. K

    Biochimie   Vol. 89   page: 1396-1408   2007

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  193. Complex formation of 70-kDa heat shock protein with acidic glycolipids and phospholipids. Reviewed

    Harada Y, Sato C, Kitajima K

    Biochem Biophys Res Commun.   Vol. 353   page: 655-660   2007

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  194. Membrane microdomain formation is crucial in epiboly during gastrulation of medaka. Reviewed

    Adachi, T, Sato, C, Kitajima, K

    Biochem. Biophys. Res. Commun.   Vol. 355   page: 174-180   2007

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  195. AMPAR removal underlies Abeta-induced synaptic depression and dendritic spine loss.

    Hsieh H, Boehm J, Sato C, Iwatsubo T, Tomita T, Sisodia S, Malinow R

    Neuron   Vol. 52 ( 5 ) page: 831-43   2006.12

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.neuron.2006.10.035

    PubMed

  196. Expression and functional importance of the carbohydrate antigen 3D11 in early embryo of medaka, Oryzias latipes

    Kitajima Ken, Hara Hiroaki, Adachi Tomoko, Sato Chihiro

    ZOOLOGICAL SCIENCE   Vol. 23 ( 12 ) page: 1175-1175   2006.12

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    Web of Science

  197. Flagellasialin: a novel sulfated alpha 2,9-linked polysialic acid glycoprotein of sea urchin sperm flagella Reviewed

    Shinji Miyata, Chihiro Sato, Hironobu Kumita, Masaru Toriyama, Victor D. Vacquier, Ken Kitajima

    GLYCOBIOLOGY   Vol. 16 ( 12 ) page: 1229 - 41   2006.12

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:OXFORD UNIV PRESS INC  

    A novel alpha 2,9-linked polysialic acid (polySia)-containing glycoprotein of sea urchin sperm flagella was identified and named "flagellasialin." Flagellasialin from Hemicentrotus pulcherrimus shows a diverse relative molecular mass on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of 40-80 kDa. Flagellasialin is a 96-amino acid, threonine-rich, heavily O-glycosylated (80-90% by weight) glycoprotein with a single transmembrane segment at its C-terminus and no apparent cytosolic domain. Of 12 extracellular Thr residues, eight are O-glycosylated and three are nonglycosylated. Flagellasialin is highly expressed in the testis but cannot be detected in the ovary. The amino acid sequences of flagellasialin from three sea urchin species (H. pulcherrimus, Strongylocentrotus purpuratus, and Strongylocentrotus franciscanus) are identical, but some species differences exist in the three core glycan structures to which the sulfated alpha 2,9-linked polyNeu5Ac chain is linked. Finally, the treatment of sperm with a specific antibody against the alpha 2,9-linked polyNeu5Ac structure results in the elevation of intracellular Ca2+ and inhibition of sperm motility and fertilization, implicating flagellasialin as a regulator of these critical processes.

    DOI: 10.1093/glycob/cwl036

    Web of Science

    Scopus

    PubMed

  198. Polysialic acid on flagellasialin is involved in sperm motility

    Miyata Shinji, Sato Chihiro, Kumita Hironobu, Toriyama Masaru, Kitajima Ken

    ZOOLOGICAL SCIENCE   Vol. 23 ( 12 ) page: 1171-1171   2006.12

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    Web of Science

  199. Involvement of the α2,8-Polysialyltransferases II/STX and IV/PST in the Biosynthesis of Polysialic Acid Chains on the O-Linked Glycoproteins in Rainbow Trout Ovary

      Vol. 140 ( 5 ) page: 687-701   2006.11

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    CiNii Article

  200. Involvement of the alpha 2,8-polysialyltransferases II/STX and IV/PST in the biosynthesis of polysialic acid chains on the O-linked glycoproteins in rainbow trout ovary

    Asahina Shinji, Sato Chihiro, Matsuno Midori, Matsuda Tsukasa, Colley Karen, Kitajima Ken

    JOURNAL OF BIOCHEMISTRY   Vol. 140 ( 5 ) page: 687-701   2006.11

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1093/jb/mvj200

    Web of Science

    Scopus

    PubMed

  201. Identification of disialic acid-containing glycoproteins in mouse serum: a novel modification of immunoglobulin light chains, vitronectin, and plasminogen Reviewed

    Z Yasukawa, C Sato, K Sano, H Ogawa, K Kitajima

    GLYCOBIOLOGY   Vol. 16 ( 7 ) page: 651 - 65   2006.7

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:OXFORD UNIV PRESS INC  

    Serum glycoproteins are involved in various biologic activities, such as the removal of exogenous antigens, fibrinolysis, and metal transport. Some of them are also useful markers of inflammation and disease. Although the amount of sialic acid increases following inflammation, little attention has been paid to the presence of linkage-specific epitopes in serum, especially the alpha 2,8-linkage. In a previous study, we demonstrated that four components in mouse serum contain alpha 2,8-linked disialic acid (diSia), based on immunoreactivity with monoclonal antibody 2-4B, which is specific to N-glycolyl-neuraminic acid (Neu5Gc)alpha 2 -&gt; (8Neu5Gc alpha 2 -&gt;)(n-1), n &gt;= 2 [Yasukawa et al., (2005) Glycobiology, 15, 827-837]. In this study, we purified three components, 30-, 70-, and 120-kDa gp, and identified them as an immunoglobulin (Ig) light chain, vitronectin, and plasminogen, respectively, using matrix-assisted laser desorption/ionization time-of-flight mass spectroscopy analyses. Modifications of these proteins with alpha 2,8-linked diSia were chemically confirmed by fluorometric C-7/C-9 analyses and mild acid hydrolysates-fluorometric anion-exchange chromatography analyses. We also demonstrated that the IgG, IgM, and IgE light chains are commonly modified with alpha 2,8-linked diSia. In addition, both mouse and rat vitronectin contained diSia, and the amount of disialylation in vitronectin dramatically decreased after hepatectomy. These results indicate that a novel diSia modification of serum glycoproteins is biologically important for immunologic events and fibrinolysis.

    DOI: 10.1093/glycob/cwj112

    Web of Science

    Scopus

    PubMed

  202. Oral ingestion of mannose alters the expression level of deaminoneuraminic acid (KDN) in mouse organs

    Go Shinji, Sato Chihiro, Furuhata Kimio, Kitajima Ken

    GLYCOCONJUGATE JOURNAL   Vol. 23 ( 5-6 ) page: 411-421   2006.7

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1007/s10719-006-6734-z

    Web of Science

    Scopus

    PubMed

  203. Weaning-induced expression of a milk-fat globule protein, MFG-E8, in mouse mammary glands, as demonstrated by the analyses of its mRNA, protein and phosphatidylserine-binding activity

    Nakatani H, Aoki N, Nakagawa Y, Jin-No S, Aoyama K, Oshima K, Ohira S, Sato C, Nadano D, Matsuda T

    BIOCHEMICAL JOURNAL   Vol. 395 ( 1 ) page: 21-30   2006.4

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1042/BJ20051459

    Web of Science

    Scopus

    PubMed

  204. Hypoxic culture induces expression of sialin, a sialic acid transporter, and cancer-associated gangliosides containing non-human sialic acid on human cancer cells

    Yin J, Hashimoto A, Izawa M, Miyazaki K, Chen GY, Takematsu H, Kozutsumi Y, Suzuki A, Furuhata K, Cheng FL, Lin CH, Sato C, Kitajima K, Kannagi R

    CANCER RESEARCH   Vol. 66 ( 6 ) page: 2937-2945   2006.3

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1158/0008-5472.CAN-05-2615

    Web of Science

    Scopus

    PubMed

  205. Hypoxic culture induces expression of Sialin, a sialic acid transporter, and cancer-associated gangliosides containing non-human sialic acid on human cancer cells. , Reviewed

    Yin, J., Hashimoto, A., Izawa, M., Miyazaki, K., Chen G-Y., Takematsu, H., Kozutsumi, Y., Suzuki, A., Furuhata, K., Cheng, F.-L. Lin, C.-H., Sato, C., Kitajima, K., and Kannagi, R.

    Cancer Res.   Vol. 66   page: 2937-2945   2006

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  206. *Identification of disialic acid-containing glycoproteins in mouse serum. Reviewed

    Yasukawa, Z., Sato, C., Sano, K., Ogawa, H. and Kitajima, K.

    Glycobiology   Vol. 15   page: 827-837   2006

     More details

    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

    Serum glycoproteins are involved in various biological activities such as removal of exogenous antigen, fibrinolysis, transport of metal. They are also useful markers of inflammation and diseases. Although it is known that the amount of sialic acid increases after inflammation, little attention has been paid to the presence of linkage specific epitope in serum,especially, the α2,8-linkage. In the previous study, we demonstrated that four components in mouse serum contain the α2,8-linked disialic acid, based on the immunoreactivity with mAb.2-4B that is specific to Neu5Gcα2→(8Neu5Gcα2→)n-1, n≥2 (Yasukawa, Z. et al. (2005) Glycobiology 15, 827-837). In this study, we purified three components, 30kDa-gp, 70kDa-gp, and 120kDa-gp and identified as the light chain of immunoglobulins, vitronectin, and plasminogen, respectively, using MALDI-TOF MS analyses. The modifications of these proteins with the α2,8-linked disialic acid was chemically confirmed by fluorometric C7/C9 analyses and acid hydrolysates-DMB methods. We also showed that the light chains of IgG, IgM and IgE are commonly modified with the α2,8-linked disialic acid. In addition, not only mouse vitronectin but also rat vitronectin was also modified with disialic acid and the amount of disialylation on vitronectin decreased after hepatectomy. These results indicate that disialic acid-modification on serum glycoprotein has some biological meanings in immunological events and fibrinolysis.

  207. Involvement of the alpha2,8-polysialyltransferases II/STX and IV/PST in the biosynthesis of polysialic acid chains on the O-linked glycoproteins in rainbow trout ovary. Reviewed

    Asahina S, Sato C, Matsuno M, Matsuda T, Colley K, Kitajima K. Asahina S, Sato C, Matsuno M, Matsuda T, Colley K, Kitajima K.

    J Biochem.   Vol. 140   page: 687-701   2006

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  208. Oral ingestion of mannose alters the expression level of deaminoneuraminic acid (KDN) in mouse organs. Reviewed

    Go, S., Sato, C., and Kitajima, K.

    Glycoconjugate J.   Vol. 23   page: 411-421   2006

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  209. Weaning-induced expression of a milk-fat globule protein, MFG-E8, in mouse mammary glands, as demonstrated by the analyses of its mRNA, protein and phosphatidylserine-binding activity. Reviewed

    Nakatani H, Aoki N, Nakagawa Y, Jin-No S, Aoyama K, Oshima K, Ohira S, Sato C, Nadano D, Matsuda T.

    Biochem J.   Vol. 395   page: 21-30   2006

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  210. Flagellasialin: a novel sulfated alpha2,9-linked polysialic acid glycoprotein of sea urchin sperm flagella. Reviewed

    Miyata S, Sato C, Kumita H, Toriyama M, Vacquier VD, Kitajima K.

    Glycobiology   Vol. 16   page: 1229-12241   2006

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    A novel α2,9-linked polysialic acid-containing glycoprotein of sea urchin sperm flagella was previously identified and named “flagellasialin". Flagellasialin from Hemicentrotus pulcherrimus shows a diverse relative molecular mass on SDS-PAGE of 40-80 kDa. Its glycan structures were determined as HSO3→8Neu5Acα2→9(Neu5Acα2→9)n-2Neu5Acα2→6GalNAcα1→Thr (n, averaging 15) [Miyata et al. (2004) Glycobiology, 14, 827-840]. In this study, we purified flagellasialin, and cloned its cDNA. Flagellasialin is a 96-amino acid, threonine-rich, heavily O-glycosylated (80-90% by weight) glycoprotein with a single transmembrane segment at its C-terminus and no apparent cytosolic domain. Of 12 extracellular Thr residues, eight are O-glycosylated and three are non-glycosylated. Flagellasialin is highly expressed in testis, but cannot be detected in ovary. The amino acid sequences of flagellasialin from three sea urchin species (H. pulcherrimus, Strongylocentrotus purpuratus and S. franciscanus) are identical, but some species differences exist in the three core glycan structures to which the sulfated α2,9-linked polyNeu5Ac chain is linked. Finally, a specific antibody against the α2,9-linked polyNeu5Ac structure inhibits sperm motility and reduces fertilization, implicating flagellasialin as a regulator of these critical processes.

  211. Weaning-induced expression of a milk-fat globule protein, MFG-E8, in mouse mammary glands, as demonstrated by the analyses of its mRNA, protein and phosphatidylserine-binding activity. Reviewed

    Nakatani H, Aoki N, Nakagawa Y, Jin-No S, Aoyama K, Oshima K, Ohira S, Sato C, Nadano D, Matsuda T

    Biochem J.   Vol. 395   page: 21-30   2006

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  212. Hypoxic culture induces expression of Sialin, a sialic acid transporter, and cancer-associated gangliosides containing non-human sialic acid on human cancer cells. , Reviewed

    Yin, J, Hashimoto, A, Izawa, M, Miyazaki, K, Chen G-Y, Takematsu, H, Kozutsumi, Y, Suzuki, A, Furuhata, K, Cheng, F.-L. Lin, C.-H, Sato, C, Kitajima, K, Kannagi, R

    Cancer Res.   Vol. 66   page: 2937-2945   2006

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  213. Involvement of the alpha2,8-polysialyltransferases II/STX and IV/PST in the biosynthesis of polysialic acid chains on the O-linked glycoproteins in rainbow trout ovary. Reviewed

    Asahina S, Sato C, Matsuno M, Matsuda T, Colley K, Kitajima, K, Asahina S, Sato C, Matsuno M, Matsuda T, Colley K, Kitajima K

    J Biochem.   Vol. 140   page: 687-701   2006

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  214. Oral ingestion of mannose alters the expression level of deaminoneuraminic acid (KDN) in mouse organs. Reviewed

    Go, S, Sato, C, Kitajima, K

    Glycoconjugate J.   Vol. 23   page: 411-421   2006

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  215. An N-terminal 130 kDa fragment of a fourth sea urchin sperm receptor for egg jelly module protein, REJ4, is secreted during acrosome reaction

    Miyata Shinji, Sato Chihiro, Toriyama Masaru, Kitajama Ken

    ZOOLOGICAL SCIENCE   Vol. 22 ( 12 ) page: 1456-1456   2005.12

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    Web of Science

  216. Capacitation-induced changes of the surface distribution of sialoglycotopes on pig sperm

    Xue Lian, Miyata Shinji, Sato Chihiro, Kitajima Ken

    ZOOLOGICAL SCIENCE   Vol. 22 ( 12 ) page: 1456-1456   2005.12

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    Web of Science

  217. Inflammation-dependent changes in alpha 2,3-, alpha 2,6-, and alpha 2,8-sialic acid glycotopes on serum glycoproteins in mice Reviewed

    Z Yasukawa, C Sato, K Kitajima

    GLYCOBIOLOGY   Vol. 15 ( 9 ) page: 827 - 37   2005.9

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:OXFORD UNIV PRESS INC  

    The expression of acute-phase serum proteins increases in response to inflammatory stimuli. Most of these proteins are glycoproteins that often contain sialic acids (Sia). It is unknown, however, how the expression of Sia in these glycoproteins changes during inflammation. This study demonstrates changes in the alpha 2,3-, alpha 2,6-, and alpha 2,8-Sia glycotopes on serum glycoproteins in response to turpentine oil-induced inflammation, based on lectin- and immunoblot analyses by using sialyl linkage-specific lectins, Maackia amurensis for the alpha 2,3-Sia glycotope and Sambucus sieboldiana for the alpha 2,6-Sia glycotopes, and monoclonal antibody 2413 (mAb.2-413) recognizing the di- and oligomers of the alpha 2,8-Neu5Gc residue. There was an increase in a limited number of sialoglycoproteins containing the alpha 2,3-, alpha 2,6-, or alpha 2,8-Sia glycotopes. Reverse transcription-polymerase chain reaction (RT-PCR) analysis of the expression profiles of mRNAs for the known sialyltransferases in mouse liver during inflammation indicated the up-regulated expression of P-galactoside alpha 2,3-sialyltransferases (ST3Gal I and ST3Gal III) and P-N-acetylgalactosaminide alpha 2,6-sialyltransferase (ST6GalNAc VI) as well as P-galactoside alpha 2,6-sialyltransferase (ST6Gal 1) mRNAs. Notably, ST3Gal I and III and ST6GalNAc VI are involved in the synthesis of the alpha 2,3- and alpha 2,6-Sia glycotopes on O-glycan chains and possibly on gangliosides, whereas ST6Gal I is specific for N-glycan chains. These results provide evidence for the inflammation-induced expression of sialyl glycotopes in serum glycoproteins. We demonstrated that inflammation significantly increased the expression of an unknown 32-kDa glycoprotein containing the alpha 2,8-Sia glycotope. The mechanism for the increase in glycoprotein in inflamed mouse serum remains to be examined, as mRNA expression for all of the alpha 2,8-sialyltransferases (ST8Sia I-VI) was unchanged during inflammation.

    DOI: 10.1093/glycob/cwi068

    Web of Science

    Scopus

    PubMed

  218. Crystallization and preliminary X-ray analysis of mitochondrial presequence receptor Tom20 in complexes with a presequence from aldehyde dehydrogenase

    Igura M, Ose T, Obita T, Sato C, Maenaka K, Endo T, Kohda D

    ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS   Vol. 61 ( 5 ) page: 514-517   2005.5

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1107/S1744309105011577

    Web of Science

    Scopus

  219. Distal myopathy with rimmed vacuoles - Impaired O-glycan formation in muscular glycoproteins

    Tajima Y, Uyama E, Go S, Sato C, Tao N, Kotani M, Hino H, Suzuki A, Sanai Y, Kitajima K, Sakuraba H

    AMERICAN JOURNAL OF PATHOLOGY   Vol. 166 ( 4 ) page: 1121-1130   2005.4

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    Web of Science

  220. Distal myopathy with rimmed vacuoles - Impaired O-glycan formation in muscular glycoproteins Reviewed

    Y Tajima, E Uyama, S Go, C Sato, N Tao, M Kotani, H Hino, A Suzuki, Y Sanai, K Kitajima, H Sakuraba

    AMERICAN JOURNAL OF PATHOLOGY   Vol. 166 ( 4 ) page: 1121 - 30   2005.4

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:AMER SOC INVESTIGATIVE PATHOLOGY, INC  

    Distal myopathy with rimmed vacuoles (DMRV), is an autosomal recessive disorder with early adult onset, displays distal dominant muscular involvement and is characterized by the presence of numerous rimmed vacuoles in the affected muscle fibers. The pathophysiology of DMRV has not been clarified yet, although the responsible gene was identified as that encoding UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase involved in the biosynthesis of sialic acids. To identify defective carbohydrate moieties of muscular glycoproteins; from DMRV patients, frozen skeletal muscle sections from seven patients with DMRV, as well as normal and pathological controls, were treated with or without sialidase or N-glycosidase F followed by lectin staining and lectin blotting analysis. The sialic acid contents of the O-glycans in the skeletal muscle specimens from the DMRV patients were also measured. We found that Aracbis hypogaea agglutinin (PNA) lectin reacted strongly with sarcolemmal glycoproteins in the DMRV patients but not with those in control subjects. a-Dystroglycan from the DMRV patients strongly associated with PNA lectin, although that from controls did not. The sialic acid level of the O-glycans in the DMRV muscular glycoproteins with molecular weights of 30 to 200 kd was reduced to 60 to 80% of the control level. The results show that impaired sialyl O-glycan formation in muscular glycoproteins, including a-dystroglycan, occurs in DMRV.

    DOI: 10.1016/S0002-9440(10)62332-2

    Web of Science

    Scopus

    PubMed

  221. Differentiation of noncancerous tissue and cancer lesions by apparent diffusion coefficient values in transition and peripheral zones of the prostate

    Sato C, Naganawa S, Nakamura T, Kumada H, Miura S, Takizawa O, Ishigaki T

    JOURNAL OF MAGNETIC RESONANCE IMAGING   Vol. 21 ( 3 ) page: 258-262   2005.3

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1002/jmri.20251

    Web of Science

    Scopus

  222. Development of a simple and efficient method for assaying cytidine monophosphate sialic acid synthetase activity using an enzymatic reduced nicotinamide adenine dinucleotide/oxidized nicotinamide adenine dinucleotide converting system Reviewed

    A Fujita, C Sato, AK Munster-Kuhnel, R Gerardy-Schahn, K Kitajima

    ANALYTICAL BIOCHEMISTRY   Vol. 337 ( 1 ) page: 12 - 21   2005.2

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:ACADEMIC PRESS INC ELSEVIER SCIENCE  

    A new reliable method to assay the activity of cytidine monophosphate sialic acid (CMP-Sia) synthetase (CSS) has been developed. The activation of sialic acids (Sia) to CMP-Sia is a prerequisite for the de novo synthesis of sialoglycoconjugates. In vertebrates, CSS has been cloned from human, mouse, and rainbow trout, and the crystal structure has been resolved for the mouse enzyme. The mouse and rainbow trout enzyme have been compared with respect to substrate specificity, demonstrating that the mouse enzyme exhibits a pronounced specificity for N-acetylneuraminic acid (Neu5Ac), while the rainbow trout CSS is equally active with either of three Sia species, Neu5Ac, N-glycolylneuraminic acid (Neu5Gc), and deaminoncuraminic acid (KDN). However, molecular details that explain the pronounced substrate specificities are unknown. Understanding the catalytic mechanisms of these enzymes is of major importance, since CSSs play crucial roles in cellular sialylation patterns and thus are potential drug targets in a number of pathophysiological situations. The availability of the cDNAs and the obtained structural data enable rational approaches; however, these efforts are limited by the lack of a reliable high-throughput assay system. Here we describe a new assay system that allows product quantification in a reduced nicotinamide adenine dinucleotide (NADH)-dependent color reaction. The activation reaction catalyzed by CSS, CTP + Sia --&gt; CMP-Sia + pyrophosphate, was evaluated by a consumption of Sia, which corresponds to that of NADH on the following two successive reactions: (i) Sia --&gt; pyruvate + ManNAc (or Man), catalyzed by a sialic acid lyase (SAL), and (ii) pyruvate + NADH --&gt; lactate + oxidized nicotinamide adenine dinucleotide (NAD+), catalyzed by a lactate dehydrogenase (LDH). Consumption of NADH can be photometrically monitored on a microtiter plate reader for a number of test samples at the same time. Furthermore, based on the quantification of CSS used in the SAL/LDH assay, relative activities toward Sia derivatives have been obtained. The preference of mouse CSS toward Neu5Ac and the ability of the rainbow trout enzyme to activate both KDN and Neu5Ac were confirmed. Thus, this simple and time-saving method is suitable for a systematic comparison of enzyme activity of structurally mutated enzymes based on the relative specific activity. (C) 2004 Elsevier Inc. All rights reserved.

    DOI: 10.1016/j.ab.2004.10.033

    Web of Science

    Scopus

    PubMed

  223. Distal Myopathy with Rimmed Vaculoes. Reviewed

    Tajima, Y., Uyama, E., Go, S., Sato, C., Tao, N., Kotani, M., Hino, H., Suzuki, A.,Sanai, Y., Kitajima, K., and Sakuraba, H.

    Am. J. Pathol.   Vol. 166   page: 1121-1128   2005

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  224. Inflammation-dependent changes in α2,3-, α2,6-, and α2,8-sialic acid glycotopes on serum glycoproteins in mouse. Reviewed

    Yasukawa, Z., Sato, C., and Kitajima, K.

    Glycobiology   Vol. 15   page: 827-837   2005

     More details

    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

  225. AN N-TERMINAL 130 KDA FRAGMENT OF A FOURTH SEA URCHIN SPERM RECEPTOR FOR EGG JELLY MODULE PROTEIN, REJ4, IS SECRETED DURING ACROSOME REACTION(Developmental Biology,Abstracts of papers presented at the 76^<th> Annual Meeting of the Zoological Society of Japan) :

    Miyata Shinji, Sato Chihiro, Toriyama Masaru, Kitajama Ken

    Zoological science   Vol. 22 ( 12 ) page: .   2005

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    CiNii Article

  226. CAPACITATION-INDUCED CHANGES OF THE SURFACE DISTRIBUTION OF SIALOGLYCOTOPES ON PIG SPERM(Developmental Biology,Abstracts of papers presented at the 76^<th> Annual Meeting of the Zoological Society of Japan) :

    Xue Lian, Miyata Shinji, Sato Chihiro, Kitajima Ken

    Zoological science   Vol. 22 ( 12 ) page: .   2005

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    CiNii Article

  227. A major ganglioside from Hemicentrotus pulcherrimus sperm specifically interacts with phospholipids

    Yamakawa Nao, Sato Chihiro, Kitajima Ken

    ZOOLOGICAL SCIENCE   Vol. 21 ( 12 ) page: 1292-1292   2004.12

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    Web of Science

  228. Cell growth stimulating activity of the peptides derived from cortical alveolus glycoproteins of fish egg (hyosophorin)

    Maeda Eri, Sato Chihiro, Kitajima Ken

    ZOOLOGICAL SCIENCE   Vol. 21 ( 12 ) page: 1292-1292   2004.12

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    Web of Science

  229. The 130 kDa glycoprotein is secreted from sperm during acrosome reaction of sea urchin, Hemicentrotus pulcherrimus

    Miyata Shinji, Sato Chihiro, Toriyama Masaru, Hirohashi Noritaka, Vacquier Victor, Kitajima Ken

    ZOOLOGICAL SCIENCE   Vol. 21 ( 12 ) page: 1292-1292   2004.12

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    Web of Science

  230. A newly identified zona pellucida glycoprotein, ZPD, and dimeric ZP1 of chicken egg envelope are involved in sperm activation on sperm-egg interaction

    Okumura H, Kohno Y, Iwata Y, Mori H, Aoki N, Sato C, Kitajima K, Nadano D, Matsuda T

    BIOCHEMICAL JOURNAL   Vol. 384 ( Pt 1 ) page: 191-199   2004.11

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1042/BJ20040299

    Web of Science

    Scopus

    PubMed

  231. Development of sensitive chemical and immunochemical methods for detecting sulfated sialic acids and their application to localization and quantitation studies of sea urchin sperm and eggs

    Yamakawa N, Maehashi E, Miyata S, Sato C, Furuhata K, Kitajima K

    GLYCOBIOLOGY   Vol. 14 ( 11 ) page: 1146-1146   2004.11

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    Web of Science

  232. Effects of exogenously administered mannose and deaminoneuraminic acid (KDN) to mice or murine cultured cells on the metabolism of KDN

    Go S, Sato C, Kitajima K

    GLYCOBIOLOGY   Vol. 14 ( 11 ) page: 1166-1167   2004.11

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    Web of Science

  233. Occurrence and characterization of a novel sulfated alpha 2,9-linked polysialic acid-containing glycoprotein in sea urchin sperm flagellum

    Miyata S, Sato C, Kitajima K

    GLYCOBIOLOGY   Vol. 14 ( 11 ) page: 1146-1146   2004.11

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    Web of Science

  234. Mouse T cell CD166 is a disialic acid-containing glycoprotein and involved in T cell activation

    Sato C, Nohara K, Matsuda T, Kitajima K

    GLYCOBIOLOGY   Vol. 14 ( 11 ) page: 1136-1136   2004.11

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    Web of Science

  235. Mouse heat shock protein 70 recognizes negatively charged glycolipids and phospholipids

    Harada Y, Sato C, Kitajima K

    GLYCOBIOLOGY   Vol. 14 ( 11 ) page: 1101-1102   2004.11

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    Web of Science

  236. Studies on chemical properties of cyclic sialic acid using their synthetic S- and O-glycosides as model compounds

    Iwata S, Sato C, Ando H, Kiso M, Kannagi R, Kitajima K

    GLYCOBIOLOGY   Vol. 14 ( 11 ) page: 1163-1163   2004.11

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    Web of Science

  237. Specificity of human trans-sialidase as probed with gangliosides

    Nikonova EY, Tertov VV, Sato C, Kitajima K, Bovin NV

    BIOORGANIC & MEDICINAL CHEMISTRY LETTERS   Vol. 14 ( 20 ) page: 5161-5164   2004.10

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.bmcl.2004.07.058

    Web of Science

    Scopus

  238. Preface for the Special Issue Entitled "New Waves in Sialobioscience"

    SATO Chihiro

    Trends in glycoscience and glycotechnology   Vol. 16 ( 91 ) page: 291-292   2004.9

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    CiNii Article

  239. A major flagellum sialoglycoprotein in sea urchin sperm contains a novel polysialic acid, an alpha 2,9-linked poly-N-acetyineuraminic acid chain, capped by an 8-O-sulfated sialic acid residue Reviewed

    S Miyata, C Sato, S Kitamura, M Toriyama, K Kitajima

    GLYCOBIOLOGY   Vol. 14 ( 9 ) page: 827 - 40   2004.9

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:OXFORD UNIV PRESS INC  

    A new type of polysialic acid (polySia) structure was demonstrated to occur in a major unknown sialoglycoprotein with a diverse molecular mass of 40-80 kDa in sea urchin sperm. The polySia-containing glycan structure was determined to be HSO3--&gt;8Neu5Acalpha2--&gt;9(Neu5Acalpha2--&gt;9)(n-2) Neu5Acalpha2--&gt;6GalNAcalpha1--&gt;Ser/Thr (n, on average 15), based on carbohydrate analysis of the sialoglycopeptide obtained by an exhaustive protease digestion of whole sperm, fluorometric anion-exchange high-performance liquid chromatography, and methylation analysis. The sulfate group was predominantly localized to the nonreducing terminus of the polySia chain. This is the first example of an alpha2,9-linked polySia structure in animal sperm. The polySia-containing sialoglycoprotein was present in sperm flagellum but not in the head. Furthermore, this sialoglycoprotein localized in the sperm lipid raft, which contains an enriched ganglioside (Neu5Acalpha2--&gt;8Neu5Acalpha2--&gt;6GlcCer), a receptor for sperm-activating peptide (speract), and its associated guanylate cyclase (Ohta et al. [2000] Glycoconj. J., 17, 205-214).

    DOI: 10.1093/glycob/cwh100

    Web of Science

    Scopus

    PubMed

  240. Chain length diversity of sialic acids and its biological significance

    Sato C

    TRENDS IN GLYCOSCIENCE AND GLYCOTECHNOLOGY   Vol. 16 ( 91 ) page: 331-344   2004.9

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    Web of Science

  241. Developmental Expression of a Sialyltransferase Responsible for Sialylation of Cortical Alveolus Glycoprotein during Oogenesis in Rainbow Trout (Oncorhynchus mykiss)

    Asahina Shinji, Sato Chihiro, Kitajima Ken

    The journal of biochemistry   Vol. 136 ( 2 ) page: 189-198   2004.8

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    CiNii Article

  242. Developmental expression of a sialyltransferase responsible for sialylation of cortical alveolus glycoprotein during oogenesis in rainbow trout (Oncorhynchus mykiss)

    Asahina S, Sato C, Kitajima K

    JOURNAL OF BIOCHEMISTRY   Vol. 136 ( 2 ) page: 189-198   2004.8

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1093/jb/mvh106

    Web of Science

    Scopus

    PubMed

    CiNii Article

  243. alpha-C-mannosyltryptophan is not recognized by conventional mannose-binding lectins

    Nishikawa T, Kajii S, Sato C, Yasukawa Z, Kitajima K, Isobe M

    BIOORGANIC & MEDICINAL CHEMISTRY   Vol. 12 ( 9 ) page: 2343-2348   2004.5

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.bmc.2004.02.009

    Web of Science

    Scopus

    PubMed

  244. 母乳中に発見された新規ポリシアロ糖タンパク質 : CD36がユニークな糖鎖修飾を受けていることが明らかに

    佐藤 ちひろ

    化学と生物   Vol. 42 ( 1 ) page: 12-14   2004.1

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    CiNii Article

  245. alpha-C-Mannosyltryptophan is not recognized by conventional mannose-binding lectins Reviewed

    T.Nishikawa, S. Kajii, C. Sato, Z. Yasukawa, K. Kitajima, and M. Isobe

    Bioorg Med Chem.     page: 2343-2348   2004

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  246. A major flagellum sialoglycoprotein in sea urchin sperm contains a novel polysialic acid, an α2,9-linked poly-N-acetylneuraminic acid chain, capped by an 8-O-sulfated sialic acid residue Reviewed

    S. Miyata, C. Sato, S. Kitamura, S. Toriyama, and K. Kitajima,

    Glycobiology   Vol. 14   page: 827-840   2004

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    A new type of polysialic acid (polySia) structure was demonstrated to occur in a major unknown sialoglycoprotein with a diverse molecular mass of 40-80 kDa in sea urchin sperm. The polySia-containing glycan structure was determined to be HSO3→8Neu5Acα2→9(Neu5Acα2→9)n-2 Neu5Acα2 →6GalNAcα1→Ser/Thr (n, on average 15), based on carbohydrate analysis of the sialoglycopeptide obtained by an exhaustive protease digestion of whole sperm, fluorometric anion-exchange high performance liquid chromatography, and methylation analysis. role in sperm activation and migration in the extracellular coats at fertilization, like polySia on neural cell adhesion molecule acts as a regulator of neural cell functions.

  247. Specificity of human trans-sialidase as probed with gangliosides. Reviewed

    Nikonova, E. Y., Tertov, V. V., Sato, C., Kitajima, K., and Bovin, N. V.

    Bioorg. Med. Chem. Lett.   Vol. 14   page: 5161-5164   2004

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  248. A newly identified zona pellucida glycoprotein, ZPD and dimeric ZP1 of chicken egg envelope are involved in sperm activation on sperm-egg interaction Reviewed

    H. Okumura, Y. Kohno, Y. Iwata, H. Mori, N. Aoki, C. Sato, K. Kitajima, D. Nadano, and T. Matsuda

    Biochem. J.   Vol. 383   page: 1-9   2004

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  249. Development of a simple and efficient method for assaying the CMP-sialic acid synthetase activity using an enzymatic NADH/NAD+ converting system Reviewed

    A. Fujita, C. Sato, A.-K. Münster-Kühnel, R. Gerardy-Schahn, and K. Kitajima

    Anal. Biochem.   Vol. 337   page: 12-21   2004

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  250. *Chain length diversity of sialic acids and its biological significance (Review) Invited Reviewed

    C. Sato

    Trends Glycosci. Glycotechnol   Vol. 16   page: 331-344   2004

     More details

    Authorship:Lead author   Language:English  

  251. Developmental Expression of a Sialyltransferase Responsible for Sialylation of Cortical Alveolus Glycoprotein During Oogenesis in Rainbow Trout (Oncorhynchus mykiss) Reviewed

    S. Asahina, C.Sato, and K. Kitajima

    J. Biochem.   Vol. 136   page: 189-198   2004

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  252. ジシアル酸・オリゴシアル酸・ポリシアル酸の生物学的機能 Invited

    佐藤ちひろ

      Vol. 3   page: 40-47   2004

     More details

    Authorship:Lead author   Language:Japanese  

  253. A MAJOR GANGLIOSIDE FROM HEMICENTROTUS PULCHERRIMUS SPERM SPECIFICALLY INTERACTS WITH PHOSPHOLIPIDS(Developmental Biology,Abstracts of papers presented at the 75^<th> Annual Meeting of the Zoological Society of Japan) :

    Yamakawa Nao, Sato Chihiro, Kitajima Ken

    Zoological science   Vol. 21 ( 12 ) page: .   2004

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    CiNii Article

  254. CELL GROWTH STIMULATING ACTIVITY OF THE PEPTIDES DERIVED FROM CORTICAL ALVEOLUS GLYCOPROTEINS OF FISH EGG (HYOSOPHORIN)(Developmental Biology,Abstracts of papers presented at the 75^<th> Annual Meeting of the Zoological Society of Japan) :

    Maeda Eri, Sato Chihiro, Kitajima Ken

    Zoological science   Vol. 21 ( 12 ) page: .   2004

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    CiNii Article

  255. THE 130 KDA GLYCOPROTEIN IS SECRETED FROM SPERM DURING ACROSOME REACTION OF SEA URCHIN, HEMICENTROTUS PULCHERRIMUS(Developmental Biology,Abstracts of papers presented at the 75^<th> Annual Meeting of the Zoological Society of Japan) :

    Miyata Shinji, Sato Chihiro, Toriyama Masaru, Hirohashi Noritaka, Vacquier Victor, Kitajima Ken

    Zoological science   Vol. 21 ( 12 ) page: .   2004

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    CiNii Article

  256. Specificity of human trans-sialidase as probed with gangliosides. Reviewed

    Nikonova, E. Y, Tertov, V. V, Sato, C, Kitajima, K, Bovin, N. V

    Bioorg. Med. Chem. Lett.   Vol. 14   page: 5161-5164   2004

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  257. ジシアル酸・オリゴシアル酸・ポリシアル酸の生物学的機能 Invited

    佐藤ちひろ

    Functional Glycomics   Vol. 3   page: 40-47   2004

     More details

    Language:Japanese   Publishing type:Research paper (scientific journal)  

  258. Developmental Expression of a Sialyltransferase Responsible for Sialylation of Cortical Alveolus Glycoprotein During Oogenesis in Rainbow Trout (Oncorhynchus mykiss) Reviewed

    S. Asahina, C.Sato, K. Kitajima

    J. Biochem.   Vol. 136   page: 189-198   2004

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  259. alpha-C-Mannosyltryptophan is not recognized by conventional mannose-binding lectins Reviewed

    T.Nishikawa, S. Kajii, C. Sato, Z. Yasukawa, K. Kitajima, M. Isobe

    Bioorg Med Chem.     page: 2343-2348   2004

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  260. A newly identified zona pellucida glycoprotein, ZPD and dimeric ZP1 of chicken egg envelope are involved in sperm activation on sperm-egg interaction Reviewed

    H. Okumura, Y. Kohno, Y. Iwata, H. Mori, N. Aoki, C. Sato, K. Kitajima, D. Nadano, T. Matsuda

    Biochem. J.   Vol. 383   page: 1-9   2004

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  261. *Chain length diversity of sialic acids and its biological significance (Review) Invited Reviewed

    C. Sato

    Trends Glycosci. Glycotechnol   Vol. 16   page: 331-344   2004

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  262. Frequent occurrence and biological significance of degree of polymerization in sialic acid

    SATO Chihiro

      Vol. 75 ( 12 ) page: 1526-1530   2003.12

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    CiNii Article

  263. [Frequent occurrence and biological significance of degree of polymerization in sialic acid]. Reviewed

    Sato C

    Seikagaku. The Journal of Japanese Biochemical Society   Vol. 75 ( 12 ) page: 1526 - 1530   2003.12

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    PubMed

  264. Frequent occurrence and biological significance of degree of polymerization in sialic acid

    Sato C.

    Seikagaku. The Journal of Japanese Biochemical Society   Vol. 75 ( 12 ) page: 1526-1530   2003.12

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    Scopus

  265. Frequent occurrence and biological significance of degree of polymerization in sialic acid

    Sato C

    SEIKAGAKU   Vol. 75 ( 12 ) page: 1526-1530   2003.12

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    Web of Science

  266. Discovery of two new polysialoglycoproteins in human and sea urchin. Linkage and length diversities of polysialic acid in animal glycoproteins

    Kitajima K, Yabe U, Miyata S, Sato CH

    GLYCOBIOLOGY   Vol. 13 ( 11 ) page: 844-844   2003.11

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    Web of Science

  267. Identification of the sea urchin 350-kDa sperm-binding protein as a new sialic acid-binding lectin that belongs to the heat shock protein 110 family - Implication of its binding to gangliosides in sperm lipid rafts in fertilization Reviewed

    E Maehashi, C Sato, K Ohta, Y Harada, T Matsuda, N Hirohashi, WJ Lennarz, K Kitajima

    JOURNAL OF BIOLOGICAL CHEMISTRY   Vol. 278 ( 43 ) page: 42050 - 7   2003.10

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC  

    The 350-kDa sperm-binding protein (SBP), a species-specific sperm-binding protein, is localized in the vitelline layer of sea urchin eggs. In this study, we have shown for the first time that sperm gangliosides are ligands for the intact glycosylated SBP. Using recombinant fragments of the SBP, the N-terminal heat shock protein 110-like domain was shown to be responsible for the binding. The intact SBP could bind various gangliosides, and the binding was sialidase-sensitive and inhibited by sialyllactose, thus indicating that it is the sialic acid-binding protein. Calcium and magnesium ions were not required but they did enhance the binding activity of SBP. The observation that bacterially expressed recombinant SBP and the sialidase-treated intact glycosylated SBP lost divalent cation-dependent enhancement of binding activity suggests that the sialylated carbohydrate moieties of the SBP may be involved in this property. Furthermore, the SBP was shown to bind sperm lipid rafts, in which gangliosides are enriched, and this binding was lost upon sialidase treatment of the lipid rafts. Finally, liposomes containing the ganglioside specifically inhibited fertilization. Taken together, these results allow us to identify SBP as a member of a new class of sialic acid-binding lectin belonging to the Hsp110 family, and indicate that SBP may be involved in interaction of sperm with the vitelline layer of the egg.

    DOI: 10.1074/jbc.M307493200

    Web of Science

    Scopus

    PubMed

  268. [Oligosialic acid: a common carbohydrate epitope in glycoproteins and glycolipids]. Reviewed

    Sato C, Kitajima K

    Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme   Vol. 48 ( 12 ) page: 1730 - 1736   2003.9

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    PubMed

  269. Polysialic acid in human milk - CD36 is a new member of mammalian polysialic acid-containing glycoprotein Reviewed

    U Yabe, C Sato, T Matsuda, K Kitajima

    JOURNAL OF BIOLOGICAL CHEMISTRY   Vol. 278 ( 16 ) page: 13875 - 80   2003.4

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC  

    The neural cell adhesion molecule and the voltage-sensitive sodium channel a-subunit are the only two molecules in mammals known to be modified by alpha-2,8-linked polysialic acid (polySia). We found a new polySia-containing glycoprotein in human milk and identified it as CD36, a member of the B class of the scavenger receptor superfamily. The polySia-containing glycan chain(s) were removed by alkaline treatment but not by peptide: N-glycanase F digestion, indicating that milk CD36 contained polySia on O-linked glycan chain(s). Polysialylation of CD36 occurs not only in human milk but also in mouse milk. However, CD36 in human platelets is not polysialylated. PolySia CD36 is secreted in milk at any lactation stage and reaches peak level at 1 month after parturition. Thus, it is suggested that polySia of milk CD36 is significant for neonatal development in terms of protection and nutrition.

    DOI: 10.1074/jbc.M300458200

    Web of Science

    Scopus

    PubMed

  270. Polysialic acid in human milk Reviewed

    U. Yabe, C. Sato, T. Matsuda, and K. Kitajima

    J. Biol. Chem.   Vol. 278   page: 13875-13880   2003

     More details

    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

  271. ポリシアル酸の鎖長多様性とタンパク質機能 Invited Reviewed

    佐藤ちひろ

    生化学   Vol. 75   page: 1526-1530   2003

     More details

    Authorship:Lead author   Language:Japanese  

  272. 糖タンパク質と糖脂質の共通糖鎖オリゴシアル酸の普遍的存在とその意義 Invited

    佐藤ちひろ、北島 健

    蛋白質・核酸・酵素   Vol. 48   page: 1730-1736   2003

     More details

    Authorship:Lead author   Language:Japanese  

  273. Identification of the sea urchin 350 kDa sperm binding protein as a new sialic acid-binding lectin that belongs to the heat shock protein 110 family. Reviewed

    E. Maehashi, C. Sato, K. Ohta, Y. Harada, T., Matsuda, N. Hirohashi, W. J. Lennarz, and K. Kitajima

    J. Biol. Chem.   Vol. 278   page: 42050-42057   2003

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  274. FORMATION OF MEMBRANE MICRODOMAINS IS SIGNIFICANT IN DEVELOPING EMBRYOS OF MEDAKA(Developmental Biology,Abstracts of papers presented at the 74^<th> Annual Meeting of the Zoological Society of Japan) :

    Adachi Tomoko, Sato Chihiro, Kitajima Ken

    Zoological science   Vol. 20 ( 12 ) page: .   2003

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    CiNii Article

  275. ポリシアル酸の鎖長多様性とタンパク質機能 Invited Reviewed

    佐藤ちひろ

    生化学   Vol. 75   page: 1526-1530   2003

     More details

    Language:Japanese   Publishing type:Research paper (scientific journal)  

  276. 糖タンパク質と糖脂質の共通糖鎖オリゴシアル酸の普遍的存在とその意義 Invited

    佐藤ちひろ, 北島 健

    蛋白質・核酸・酵素   Vol. 48   page: 1730-1736   2003

     More details

    Language:Japanese   Publishing type:Research paper (scientific journal)  

  277. Neuronal differentiation-dependent expression of the disialic acid epitope on CD166 and its involvement in neurite formation in neuro2A cells

    Sato C, Matsuda T, Katajima K

    JOURNAL OF BIOLOGICAL CHEMISTRY   Vol. 277 ( 47 ) page: 45299-45305   2002.11

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1074/jbc.M206046200

    Web of Science

    Scopus

    PubMed

  278. *Neuronal differentiation-dependent expression of the disialic acid epitope on CD166 and its involvement in neurite formation in Neuro2A cells. Reviewed

    C. Sato, T. Matsuda, and K. Kitajima

    J. Biol. Chem.   Vol. 277   page: 45299-45303   2002

     More details

    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

  279. FUNCTIONS OF THE COMMON GLYCOTOPES OF GLYCOLIPIDS AND GLYCOPROTEINS IN THE MEMBRANE MICRODOMAIN OF SEA URCHIN SPERM(Developmental Biology)(Proceedings of the Seventy-Third Annual Meeting of the Zoological Society of Japan) :

    Miyata Shinji, Maehashi Eri, Ohta Kaoru, Sato Chihiro, Matsuda Tsukasa, Kitajima Ken

    Zoological science   Vol. 19 ( 12 ) page: .   2002

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    CiNii Article

  280. *Neuronal differentiation-dependent expression of the disialic acid epitope on CD166 and its involvement in neurite formation in Neuro2A cells. Reviewed

    C. Sato, T. Matsuda, K. Kitajima

    J. Biol. Chem.   Vol. 277   page: 45299-45303   2002

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  281. Occurrence of oligosialic acids on integrin alpha(5) subunit and their involvement in cell adhesion to fibronectin

    Nadanaka S, Sato C, Kitajima K, Katagiri K, Irie S, Yamagata T

    JOURNAL OF BIOLOGICAL CHEMISTRY   Vol. 276 ( 36 ) page: 33657-33664   2001.9

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    Web of Science

  282. Occurrence of Oligosialic Acids on Integrin α<inf>5</inf> Subunit and Their Involvement in Cell Adhesion to Fibronectin

      Vol. 276 ( 36 ) page: 33657-33664   2001.9

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1074/jbc.M011100200

    Scopus

  283. Identification and adipocyte differentiation-dependent expression of the unique disialic acid residue in an adipose tissue-specific glycoprotein, adipo Q

    Sato C, Yasukawa Z, Honda N, Matsuda T, Kitajima K

    JOURNAL OF BIOLOGICAL CHEMISTRY   Vol. 276 ( 31 ) page: 28849-28856   2001.8

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    Web of Science

  284. Identification and Adipocyte Differentiation-dependent Expression of the Unique Disialic Acid Residue in an Adipose Tissue-specific Glycoprotein, Adipo Q

    Sato C.

    Journal of Biological Chemistry   Vol. 276 ( 31 ) page: 28849-28856   2001.8

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1074/jbc.M104148200

    Scopus

  285. Occurrence of oligosialic acids on integrin a5 subunit and their involvement in cell adhesion to fibronectin. Reviewed

    "S. Nadanaka, C. Sato, K. Kitajima, K. Katagiri, S. Irie, and T. Yamagata 276 (2001) in press"

    J. Biol. Chem.   Vol. 276   page: 33657-33664   2001.1

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  286. Identification and adipocyte differentiation-dependent expression of the unique disialic acid residues in an adipose tissue specific glycoprotein, adipo Q Reviewed

    "C. Sato, Z. Yasukawa, N. Honda, T. Matsuda, and K. Kitajima"

    J. Biol. Chem.   Vol. 276   page: 28849-28856   2001.1

     More details

    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

  287. Identification and adipocyte differentiation-dependent expression of the unique disialic acid residues in an adipose tissue specific glycoprotein, adipo Q Reviewed

    C. Sato, Z. Yasukawa, N. Honda, T. Matsuda, K. Kitajima

    J. Biol. Chem.   Vol. 276   page: 28849-28856   2001.1

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  288. Occurrence of oligosialic acids on integrin a5 subunit and their involvement in cell adhesion to fibronectin. Reviewed

    S. Nadanaka, C. Sato, K. Kitajima, K. Katagiri, S. Irie, T. Yamagat, in press

    J. Biol. Chem.   Vol. 276   page: 33657-33664   2001.1

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  289. The Occurrence and Significance of Sialic Acids in Insects.

    Schauer Roland

    Trends in Glycoscience and Glycotechnology   Vol. 13 ( 73 ) page: 507-517   2001

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    Sialic acids, mainly <i>N</i>-acetylneuraminic acid, have been found during the development of a few insect species. In <i>Drosophila melanogaster</i> and the cicada <i>Philaenus spumarius</i> we detected polysialic acids, using lectins, antibodies, and various methods for the isolation and analysis of the monosaccharide subunits, in areas of neuronal development and in Malpighian tubules, respectively. However, sialic acids were not found in the adult animals of these species. Although the expression of these acidic sugars has unequivocally been demonstrated <i>in vivo</i>, their occurrence in insect cell cultures was, in most cases, not clearly demonstrated. The biosynthesis of sialic acids in cell cultures would be of great biotechnological significance, since the production of recombinant glycoproteins with mammalian type, complex, sialylated <i>N</i>-glycans using the baculovirus expression system would be of great benefit. From the studies dedicated to this problem it appears that the engineering of such glycans may only be possible by the expression of exogenous genes in insect cells. Several genes encoding sialic acid-metabolizing enzymes have therefore been transfected. The work on insect cells shows that sialic acids are not only restricted to the Deuterostomia branch of the animal kingdom but also occur in some Protostomia species, as in insects. This throws new light on the evolution of this acidic sugar.

    DOI: 10.4052/tigg.13.507

    CiNii Article

  290. Co-localization of receptor and transducer proteins in the glycosphingolipid-enriched, low density, detergent-insoluble membrane fraction of sea urchin sperm

    Ohta K.

    Glycoconjugate Journal   Vol. 17 ( 3-4 ) page: 205-214   2000.12

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1023/A:1026589223811

    Scopus

  291. Frequent occurrence of pre-existing alpha 2 -> 8-linked disialic and oligosialic acids with chain lengths up to 7 Sia residues in mammalian brain glycoproteins - Prevalence revealed by highly sensitive chemical methods and anti-di-, oligo-, and poly-Sia antibodies specific for defined chain lengths

    Sato C, Fukuoka H, Ohta K, Matsuda T, Koshino R, Kobayashi K, Troy FA, Kitajima K

    JOURNAL OF BIOLOGICAL CHEMISTRY   Vol. 275 ( 20 ) page: 15422-15431   2000.5

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    Web of Science

  292. Frequent occurrence of pre-existing α2→8-linked disialic and oligosialic acids with chain lengths up to 7 Sia residues in mammalian brain glycoproteins. Prevalence revealed by highly sensitive chemical methods and anti-di-, oligo-, and poly-Sia antibodies specific for defined chain lengths

      Vol. 275 ( 20 ) page: 15422-15431   2000.5

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1074/jbc.275.20.15422

    Scopus

  293. O-Acetylated Sialic Acids-Their Detection, Biological Significance and Alteration in Diseases

    SINHA D., CHATTERJEE M, MANDAL C.

    Trends in Glycoscience and Glycotechnology   Vol. 12 ( 63 ) page: 17-33   2000.1

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    Sialic acids are acidic, 9-carbon carboxylated monosaccharides, typically expressed on the outermost end of the sugar chains of membrane and secreted glycoconjugates. This particular location provides it accessibility reflected in its regulation of a multitude of cellular and molecular interactions. Amongst the diverse structural variations that occur, the commonest is <i>O</i>-acetylation at C-<sub>4, 7, 8</sub> and<sub>9</sub> carbon positions. This diversity in <i>O</i>-acetylated sialic acid (<i>O</i>-AcSA) derivatives is attributed to their linkage to the underlying sugar chain, the carbon position that gets <i>O</i>-acetylated and nature of the glycoconjugates to which they are attached. Steady refinement of analytical techniques has allowed for identification of <i>O</i>-AcSA derivatives in several diseases leading to a shift in focus towards understanding the biological importance of this modification. The present review deals primarily with the recently evolved information regarding detection of altered <i>O</i>-acetylation in various pathophysiological conditions and their biological relevance. Particular attention is focused on two diverse diseases, namely, Acute Lymphoblastic Leukemia (ALL) and Visceral Leishmaniasis (VL) in which 9-<i>O</i> acetylated sialoglycoconjugates (9-<i>O</i>AcSGs) have proved to be effective biomarkers for monitoring their clinical status.

    DOI: 10.4052/tigg.12.17

    CiNii Article

  294. *Frequent occurrence of pre-existing alpha2,8-linked disialic and oligosialic acids with chain lengths up to 7 Sia residues in mammalian brain glycoproteins. Reviewed

    "C. Sato, H. Fukuoka, K. Ohta, T. Matsuda, R. Koshino, K. Kobayashi, F. A. Troy II, and K. Kitajima"

    J. Biol. Chem.   Vol. 275 (20)   page: 15422-15431   2000.1

     More details

    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

  295. Co-localization of receptor and transducer proteins in the glycosphingolipid-enriched, low density, detergent-insoluble membrane fraction of sea urchin sperm. Reviewed

    "K. Ohta,, C. Sato, T. Matsuda, M. Toriyama, V. D. Vacquier, W. J.Lennarz, and K. Kitajima"

    Glycoconjugate J.   Vol. 17   page: 205-214   2000.1

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  296. *Frequent occurrence of pre-existing alpha2,8-linked disialic and oligosialic acids with chain lengths up to 7 Sia residues in mammalian brain glycoproteins. Reviewed

    C. Sato, H. Fukuoka, K. Ohta, T. Matsuda, R. Koshino, K. Kobayashi, F. A. Troy II, K. Kitajima

    J. Biol. Chem.   Vol. 275 (20)   page: 15422-15431   2000.1

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  297. Co-localization of receptor and transducer proteins in the glycosphingolipid-enriched, low density, detergent-insoluble membrane fraction of sea urchin sperm. Reviewed

    K. Ohta, C. Sato, T. Matsuda, M. Toriyama, V. D. Vacquier, W. J.Lennarz, K. Kitajima

    Glycoconjugate J.   Vol. 17   page: 205-214   2000.1

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  298. Co-localization of receptor and transducer proteins in the glycosphingolipid-enriched, low density, detergent-insoluble membrane fraction of sea urchin sperm

    Ohta K, Sato C, Matsuda T, Toriyama M, Vacquier VD, Lennarz WJ, Kitajima K

    GLYCOCONJUGATE JOURNAL   Vol. 17 ( 3-4 ) page: 205-214   2000

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    Web of Science

  299. Lipid raft on gametic cells as a functional domain for sperm-egg interaction coupled with signal transduction

    Ohta K.

    Zygote   Vol. 8 ( SUPPL. ) page: .   2000

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1017/s0967199400130321

    Scopus

  300. Lipid raft on gametic cells as a functional domain for sperm-egg interaction coupled with signal transduction

    Ohta K, Sato C, Matsuda T, Toriyama M, Vacquier VD, Hirohashi N, Lennarz WJ, Kitajima K

    ZYGOTE   Vol. 8   page: S63-S63   2000

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    Web of Science

  301. Lipid raft on gametic cells as a functional domain for sperm-egg interaction coupled with signal transduction Reviewed

    K Ohta, C Sato, T Matsuda, M Toriyama, VD Vacquier, N Hirohashi, WJ Lennarz, K Kitajima

    ZYGOTE   Vol. 8   page: S63 - S63   2000

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:CAMBRIDGE UNIV PRESS  

    Web of Science

  302. Glycobiology of di- and oligosialyl glycotopes

    Sato C, Kitajima K

    TRENDS IN GLYCOSCIENCE AND GLYCOTECHNOLOGY   Vol. 11 ( 62 ) page: 371-390   1999.11

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    Web of Science

  303. Glycobiology of Di- and Oligosialyl Glycotopes

    SATO Chihiro, KITAJIMA Ken

    Trends in Glycoscience and Glycotechnology   Vol. 11 ( 62 ) page: 371-390   1999.11

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    &alpha;2&rarr;8-Linked di- and oligosialic acid (diSia and oligoSia) chains with DP 2, 3 Sia residues are known to be common structural units of gangliosides, and to be involved in various biological processes, such as cell adhesion, cell differentiation, signal transduction, and surface expression of stage specific antigen. In contrast, little attention has been paid to the occurrence and functions of such short sialyl oligomers on glycoproteins. However, it has recently been shown that glycoproteins containing di- and oligoSia groups occur in nature more frequently than was ever recognized, as analytical methods to detect di- and oligoSia structures have improved. It is thus hypothesized that these di- and oligoSia moieties on glycoproteins may have similar important functions in common with those proposed for the gangliosides. In this review, we describe the recent advances in the study of di- and oligoSia residues on glycoproteins, including analytical methods, occurrence, functions, and biosynthetic pathways.

    DOI: 10.4052/tigg.11.371

    Scopus

    CiNii Article

  304. Cholesterol-dependent localization of NAP-22 on a neuronal membrane microdomain (Raft)

    Maekawa S, Sato C, Kitajima K, Funatsu N, Kumanogoh H, Sokawa Y

    JOURNAL OF BIOLOGICAL CHEMISTRY   Vol. 274 ( 30 ) page: 21369-21374   1999.7

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    Web of Science

  305. Cholesterol-dependent localization of NAP-22 on a neuronal membrane microdomain (Raft)

    Maekawa S.

    Journal of Biological Chemistry   Vol. 274 ( 30 ) page: 21369-21374   1999.7

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1074/jbc.274.30.21369

    Scopus

  306. Isolation and characterization of low density detergent-insoluble membrane (LD-DIM) fraction from sea urchin sperm

    Ohta K, Sato C, Matsuda T, Toriyama M, Lennarz WJ, Kitajima K

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   Vol. 258 ( 3 ) page: 616-623   1999.5

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    Web of Science

  307. Isolation and characterization of low density detergent-insoluble membrane (LD-DIM) fraction from sea urchin sperm

    Ohta K.

    Biochemical and Biophysical Research Communications   Vol. 258 ( 3 ) page: 616-623   1999.5

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1006/bbrc.1999.0686

    Scopus

  308. A 42-kDa glycoprotein from chicken egg-envelope, an avian homolog of the ZPC family glycoproteins in mammalian zona pellucida. Its first identification, cDNA cloning and granulosa cell-specific expression

    Takeuchi Y.

    European Journal of Biochemistry   Vol. 260 ( 3 ) page: 736-742   1999.3

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1046/j.1432-1327.1999.00203.x

    Scopus

  309. A 42-kDa glycoprotein from chicken egg-envelope, an avian homolog of the ZPC family glycoproteins in mammalian zona pellucida - Its first identification, cDNA cloning and granulosa cell-specific expression

    Takeuchi Y, Nishimura K, Aoki N, Adachi T, Sato C, Kitajima K, Matsuda T

    EUROPEAN JOURNAL OF BIOCHEMISTRY   Vol. 260 ( 3 ) page: 736-742   1999.3

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    Web of Science

  310. Fluorescent-assisted detection of oligosialyl units in glycoconjugates

    Sato C, Inoue S, Matsuda T, Kitajima K

    ANALYTICAL BIOCHEMISTRY   Vol. 266 ( 1 ) page: 102-109   1999.1

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    Web of Science

  311. Fluorescent-assisted detection of oligosialyl units in glycoconjugates

    Sato C.

    Analytical Biochemistry   Vol. 266 ( 1 ) page: 102-109   1999.1

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1006/abio.1998.2921

    Scopus

  312. A 42-kDa glycoprotein from chicken egg-envelope, an avian homolog of the ZPC family glycoproteins in mammalian zona pellucida. Its first identification, cDNA cloning and granulosa cell-specific expression. Reviewed

    "T. Takeuchi, K. Nishimura, N. Aoki, T. Adachi, C. Sato, K. Kitajima, and T. Matsuda"

    Eur. J. Biochem.   Vol. 273   page: 21526-21530   1999.1

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  313. Cholesterol-dependent localization of NAP-22 on a neuronal membrane microdomain (Raft). Reviewed

    "S. Maekawa, C. Sato, K. Kitajima, H. Kumanogoh, N. Funatsu, and Y. Sokawa"

    J. Biol. Chem.   Vol. 274   page: 21369-21374   1999.1

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  314. Isolation and characterization of low density detergent-insoluble membrane (LD-DIM) fraction from sea urchin sperm. Reviewed

    "K. Ohta, C. Sato, T. Matsuda, M. Toriyama, W. J. Lennarz, and K. Kitajima"

    Biochem. Biophys. Res. Commun.   Vol. 258   page: 616-623   1999.1

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  315. Fluorescent-assisted detection of oligosialyl units in glycoconjugates. Reviewed

    "C. Sato, S. Inoue, T. Matsuda, and K. Kitajima"

    Anal. Biochem.   Vol. 267   page: 102-109   1999.1

     More details

    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

  316. Glycobiology of di and oligosialyl glycotopes. Invited Reviewed

    "C. Sato, and K. Kitajima"

    Trends Glycosci. Glycotechnol.   Vol. 11   page: 371-390   1999.1

     More details

    Authorship:Lead author   Language:English  

  317. A 42-kDa glycoprotein from chicken egg-envelope, an avian homolog of the ZPC family glycoproteins in mammalian zona pellucida. Its first identification, cDNA cloning and granulosa cell-specific expression. Reviewed

    T. Takeuchi, K. Nishimura, N. Aoki, T. Adachi, C. Sato, K. Kitajima, T. Matsuda

    Eur. J. Biochem.   Vol. 273   page: 21526-21530   1999.1

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  318. Glycobiology of di and oligosialyl glycotopes. Invited Reviewed

    C. Sato, K. Kitajima

    Trends Glycosci. Glycotechnol.   Vol. 11   page: 371-390   1999.1

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  319. Fluorescent-assisted detection of oligosialyl units in glycoconjugates. Reviewed

    C. Sato, S. Inoue, T. Matsuda, K. Kitajima

    Anal. Biochem.   Vol. 267   page: 102-109   1999.1

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  320. Cholesterol-dependent localization of NAP-22 on a neuronal membrane microdomain (Raft). Reviewed

    S. Maekawa, C. Sato, K. Kitajima, H. Kumanogoh, N. Funatsu, Y. Sokawa

    J. Biol. Chem.   Vol. 274   page: 21369-21374   1999.1

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  321. Isolation and characterization of low density detergent-insoluble membrane (LD-DIM) fraction from sea urchin sperm. Reviewed

    K. Ohta, C. Sato, T. Matsuda, M. Toriyama, W. J. Lennarz, K. Kitajima

    Biochem. Biophys. Res. Commun.   Vol. 258   page: 616-623   1999.1

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  322. Development of a highly sensitive chemical method for detecting alpha 2 -> 8-linked oligo/polysialic acid residues in glycoproteins blotted on the membrane

    Sato C, Inoue S, Matsuda T, Kitajima K

    ANALYTICAL BIOCHEMISTRY   Vol. 261 ( 2 ) page: 191-197   1998.8

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    Web of Science

  323. Development of a highly sensitive chemical method for detecting α2→8- linked oligo/polysialic acid residues in glycoproteins blotted on the membrane

      Vol. 261 ( 2 ) page: 191-197   1998.8

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1006/abio.1998.2718

    Scopus

  324. Identification of oligo-N-glycolylneuraminic acid residues in mammal- derived glycoproteins by a newly developed immunochemical reagent and biochemical methods

    Sato C.

    Journal of Biological Chemistry   Vol. 273 ( 5 ) page: 2575-2582   1998.1

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1074/jbc.273.5.2575

    Scopus

  325. Identification of oligo-N-glycolylneuraminic acid residues in mammal-derived glycoproteins by a newly developed immunochemical reagent and biochemical methods

    Sato C, Kitajima K, Inoue S, Inoue Y

    JOURNAL OF BIOLOGICAL CHEMISTRY   Vol. 273 ( 5 ) page: 2575-2582   1998.1

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    Web of Science

  326. Development of a highly sensitive chemical method to detect a2ョ8-linked oligo/polysialic acid residues in glycoproteins blotted on the membrane. Reviewed

    "C. Sato, S. Inoue, T. Matsuda, and K. Kitajima"

    Anal. Biochem.   Vol. 261   page: 191-197   1998.1

     More details

    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

  327. Identification of oligo-N-glycolylneuraminic acid residues in mammal-derived glycoproteins by a newly developed immunochemical reagent and biochemical methods. Reviewed

    "C. Sato, K. Kitajima, S. Inoue, and Y. Inoue"

    J. Biol. Chem.   Vol. 273   page: 2575-2582   1998.1

     More details

    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

  328. Development of a highly sensitive chemical method to detect a2ョ8-linked oligo/polysialic acid residues in glycoproteins blotted on the membrane. Reviewed

    C. Sato, S. Inoue, T. Matsuda, K. Kitajima

    Anal. Biochem.   Vol. 261   page: 191-197   1998.1

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  329. Identification of oligo-N-glycolylneuraminic acid residues in mammal-derived glycoproteins by a newly developed immunochemical reagent and biochemical methods. Reviewed

    C. Sato, K. Kitajima, S. Inoue, Y. Inoue

    J. Biol. Chem.   Vol. 273   page: 2575-2582   1998.1

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  330. Occurrence of terminal α2 → 8-linked disialylated poly-N-acetyllactosamine chains with Le(X) and I antigenic glycotopes in tetraantennary arms of an N-linked glycoprotein isolated from rainbow trout ovarian fluid

      Vol. 7 ( 2 ) page: 195-205   1997.3

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1093/glycob/7.2.195

    Scopus

  331. Occurrence of terminal alpha 2-&gt;8-linked disialylated poly-N-acetyllactosamine chains with Le(X) and I antigenic glycotopes in tetraantennary arms of an N-linked glycoprotein isolated from rainbow trout ovarian fluid Reviewed

    Y Funakoshi, T Taguchi, C Sato, K Kitajima, S Inoue, HR Morris, A Dell, Y Inoue

    GLYCOBIOLOGY   Vol. 7 ( 2 ) page: 195 - 205   1997.3

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:OXFORD UNIV PRESS  

    The Pronase digestion of a 54K glycoprotein present in ovarian fluid of rainbow trout yielded a major glycopeptide, Carbohydrate compositional analysis revealed that this glycopeptide was likely to possess a single large N-glycan chain having low molecular weight oligomers of N-acetylneuraminic acid (oligoNeu5Ac), Structural studies of this glycopeptide revealed novel alpha 2 --&gt; 8-linked disialylated poly-N-acetyllactosamine chains with Le(X) and I antigenic determinants on the N-linked tetraantennary core glycan, In our recent studies (Kitazume,S., Kitajima,K., Inoue,S., Inoue,Y. and Troy,F.A. (1994) J., Biol, Chem, 269, 10330-10340) we presented evidence that synthesis of alpha 2 --&gt; 8-linked polysialic acid (polySia) chains is a two-step process in which chain initiation is catalyzed by an alpha 2 --&gt; 8-sialyltransferase (alpha 2 --&gt; 8-ST; initiase) that catalyzes synthesis of the first Sia alpha 2 --&gt; 8-linkage, forming the disialic acid (diSia) unit, Sia alpha 2 --&gt; 8-Sia alpha 2 --&gt; 6-Gal-.Chain polymerization is then postulated to be catalyzed by a second enzyme, an alpha 2 --&gt; 8-polyST (''polymerase'') that converts the diSia units to polySia chains, The present structural studies leading to the discovery of alpha 2 --&gt; 8-linked disialylated units that terminate poly-N-acetyllactosamine chains in an N-linked glycoprotein is further evidence in support of our hypothesis that more than one sialyltransferase activity is required for polySia chain synthesis and polymerization.

    Web of Science

  332. Occurrence of terminal alpha2,8-linked disialylated poly-N-acetyllactosamine chains with LeX and I antigenic glycotopes in tetraantennary arms of an N-linked glycoprotein isolated from rainbow trout ovarian fluid. Reviewed

    "Y. Funakoshi, T. Taguchi, C. Sato, K. Kitajima, S. Inoue, H. R. Morris, A. Dell, and Y. Inoue"

    Glycobiology   Vol. 7   page: 195-205   1997.1

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  333. Characterization of the antigenic specificity of four different anti-(alpha2,8-linked polysialic acid) antibodies using lipid-conjugated oligo/polysialic acids. Reviewed

    "C. Sato, K. Kitajima, S. Inoue, T. Seki, F. A. Troy, II, and Y. Inoue"

    J. Biol. Chem.   Vol. 270   page: 18923-18928   1995.1

     More details

    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

  334. Characterization of the antigenic specificity of four different anti-(alpha2,8-linked polysialic acid) antibodies using lipid-conjugated oligo/polysialic acids. Reviewed

    C. Sato, K. Kitajima, S. Inoue, T. Seki, F. A. Troy, II, Y. Inoue

    J. Biol. Chem.   Vol. 270   page: 18923-18928   1995.1

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  335. Structural diversity in a2,8-linked polysialic acid chains in salmonid fish egg glycoproteins. Reviewed

    C. Sato, K. Kitajima, I. Tazawa, Y. Inoue, S. Inoue, and F. A. Troy, II

    J. Biol. Chem.   Vol. 268   page: 23675-23684   1993

     More details

    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

  336. Structural diversity in a2,8-linked polysialic acid chains in salmonid fish egg glycoproteins. Reviewed

    C. Sato, K. Kitajima, I. Tazawa, Y. Inoue, S. Inoue, F. A. Troy, II

    J. Biol. Chem.   Vol. 268   page: 23675-23684   1993

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

▼display all

Books 39

  1. 糖鎖生物学 : 生命現象と糖鎖情報

    北島 健, 佐藤 ちひろ, 門松 健治, 加藤 晃一( Role: Joint author)

    名古屋大学出版会  2020  ( ISBN:9784815809812

     More details

    Language:Japanese Book type:Scholarly book

    CiNii Books

  2. Advanced Technologies in Sialic Acid and Sialoglycoconjugate Analysis in Top Curr Chem

    Kitajima K, Varki N, Sato C.( Role: Joint author)

    Springer  2015 

     More details

    Language:English

  3. Advanced Technologies in Sialic Acid and Sialoglycoconjugate Analysis in Top Curr Chem

    Kitajima K, Varki N, Sato C( Role: Joint author)

    Springer  2015 

     More details

    Responsible for pages:75-103   Language:English

  4. Frontal Affinity Chromatography in the Methods in Molecular Biology

    Sato, C. ( Role: Sole author)

    Springer  2014 

     More details

    Language:English

  5. Flagellasialin in the Glycoscience: Biology and Medicine

    Kanazawa, T., Suzuki, E., Miyata, S., Sato, C. and Kitajima, K. ( Role: Joint author)

    Springer  2014 

     More details

    Language:English

  6. Polysialyltransferase assay in the Glycoscience: Biology and Medicine

    Hane, M., Kitajima, K. and Sato, C. ( Role: Joint author)

    Springer  2014 

     More details

    Language:English

  7. Polysialic acid in the Glycoscience: Biology and Medicine

    Sato, C. and Kitajima, K. ( Role: Joint author)

    Springer,  2014 

     More details

    Language:English

  8. ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 2 (ST8SIA2)in Handbook of Glycosyltransferases and related genes

    Sato C( Role: Sole author)

    Springer  2014 

     More details

    Language:English

  9. ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 2 (ST8SIA2)in Handbook of Glycosyltransferases and related genes

    Sato C( Role: Sole author)

    Springer  2014 

     More details

    Responsible for pages:781-795   Language:English

  10. Flagellasialin in the Glycoscience: Biology and Medicine

    Kanazawa, T, Suzuki, E, Miyata, S, Sato, C, Kitajima, K( Role: Joint author)

    Springer  2014 

     More details

    Responsible for pages:1-6   Language:English

  11. Frontal Affinity Chromatography in the Methods in Molecular Biology

    Sato, C( Role: Sole author)

    Springer  2014 

     More details

    Responsible for pages:257-264   Language:English

  12. Polysialic acid in the Glycoscience: Biology and Medicine

    Sato, C, Kitajima, K( Role: Joint author)

    Springer,  2014 

     More details

    Responsible for pages:1-7   Language:English

  13. Polysialyltransferase assay in the Glycoscience: Biology and Medicine

    Hane, M, Kitajima, K, Sato, C( Role: Joint author)

    Springer  2014 

     More details

    Responsible for pages:1-5   Language:English

  14. Sialobiology: Structure, Function and Biosynthesis

    Sato C( Role: Joint author)

    Bentham Science  2013 

     More details

    Language:English

  15. Sialobiology: Structure, Function and Biosynthesis

    Sato C( Role: Joint author)

    Bentham Science  2013 

     More details

    Responsible for pages:33-75   Language:English

  16. Methods in Enzymology・ Analysis of glycan-protein interaction by frontal affinity chromatography and Biacore.

    Sato, C., Yamakawa, N., and Kitajima, K.( Role: Joint author)

    Elsevir science  2010 

     More details

    Language:English

  17. Methods in Enzymology・ Analysis of glycan-protein interaction by frontal affinity chromatography and Biacore.

    Sato, C, Yamakawa, N, Kitajima, K( Role: Joint author)

    Elsevir science  2010 

     More details

    Responsible for pages:219-232   Language:English

  18. Carbohydrate Recognition: Biological problems, methods, and applications

    Ken Kitajima and Chihiro Sato (Editors: Binghe Wang, Geer-Jan Boons)( Role: Joint author)

    John Wiley and Sons  2009 

     More details

    Language:English

  19. Carbohydrate Recognition: Biological problems, methods, and applications

    Ken Kitajima, Chihiro Sato, Binghe Wang, Geer-Jan Boons( Role: Joint author)

    John Wiley and Sons  2009 

     More details

    Responsible for pages:in press   Language:English

  20. Membrane microdomain as a platform of carbohydrate-mediated interactions during early development of medaka fish.

    Adachi, T., Sato, C., and Kitajima, K.( Role: Joint author)

    KINPO-DO  2008 

     More details

    Language:English

  21. Glycoscience Lab Manual・Structure analysis of oligo/polysialic acid

    C. Sato and K. Kitajima( Role: Joint author)

    KINPO-DO  2008 

     More details

    Language:English

  22. Glycoscience Lab Manual・Labeling of oligosialic acid

    C. Sato( Role: Joint author)

    KINPO-DO  2008 

     More details

    Language:English

  23. Glycoscience Lab Manual・Labeling of oligosialic acid

    C. Sato( Role: Joint author)

    KINPO-DO  2008 

     More details

    Responsible for pages:24-27   Language:English

  24. Glycoscience Lab Manual・Structure analysis of oligo/polysialic acid

    C. Sato, K. Kitajima( Role: Joint author)

    KINPO-DO  2008 

     More details

    Responsible for pages:77-81   Language:English

  25. Membrane microdomain as a platform of carbohydrate-mediated interactions during early development of medaka fish.

    Adachi, T, Sato, C, Kitajima, K( Role: Joint author)

    KINPO-DO  2008 

     More details

    Responsible for pages:299-302   Language:English

  26. 遺伝子医学MOOK 糖鎖と病気・ポリシアル酸

    佐藤ちひろ、北島 健( Role: Joint author)

    メディカルデュー  2005 

     More details

    Language:Japanese

  27. 未来を拓く糖鎖科学・オリゴシアル酸の標識

    ( Role: Joint author)

    金芳堂  2005 

     More details

    Language:Japanese

    オリゴシアル酸の標識

  28. 未来を拓く糖鎖科学・オリゴシアル酸の標識

    ( Role: Joint author)

    金芳堂  2005 

     More details

    Responsible for pages:12-13   Language:Japanese

    オリゴシアル酸の標識

  29. 遺伝子医学MOOK 糖鎖と病気・ポリシアル酸

    佐藤ちひろ, 北島 健( Role: Joint author)

    メディカルデュー  2005 

     More details

    Responsible for pages:186-191   Language:Japanese

  30. Recent Research Developments in Analytical Biochemistry・Detection of di/oligo/polysialic acid units in glycoproteins

    "C. Sato, and K. Kitajima"( Role: Joint author)

    Transworld research network  2002 

     More details

    Language:English

  31. Recent Research Developments in Analytical Biochemistry・Detection of di/oligo/polysialic acid units in glycoproteins

    C. Sato, K. Kitajima( Role: Joint author)

    Transworld research network  2002 

     More details

    Responsible for pages:201-215   Language:English

  32. Leukocyte typing VII・Carbohydrate-recognizing monoclonal antibodies as revealed by ELISA using phosphatidylethanolamine-conjugated oligosaccharides as antigens

    C. Sato and K. Kitajima( Role: Joint author)

    OXFORD  2001 

     More details

    Language:English

  33. Leukocyte typing VII・Oligosialic acid as a differentiation-associated antigen in human haematopoietic cell line HL60.

    C. Sato and K. Kitajima( Role: Joint author)

    OXFORD  2001 

     More details

    Language:English

  34. Leukocyte typing VII・Carbohydrate-recognizing monoclonal antibodies as revealed by ELISA using phosphatidylethanolamine-conjugated oligosaccharides as antigens

    C. Sato, K. Kitajima( Role: Joint author)

    OXFORD  2001 

     More details

    Responsible for pages:168-169   Language:English

  35. Leukocyte typing VII・Oligosialic acid as a differentiation-associated antigen in human haematopoietic cell line HL60.

    C. Sato, K. Kitajima( Role: Joint author)

    OXFORD  2001 

     More details

    Responsible for pages:208-211   Language:English

  36. Sialobiology and Other Novel Form of Glycosylation・Occurrence of a2,8-linked oligosialic acid residues in mammalian glycoproteins.

    K. Kitajima, C. Sato, N. Honda, T. Matsuda, M.-H. Yokoyama, B. E. Close, and K. J. Colley( Role: Joint author)

    Gakushin  1999 

     More details

    Language:English

  37. Sialobiology and Other Novel Form of Glycosylation・Occurrence of a2,8-linked oligosialic acid residues in mammalian glycoproteins.

    K. Kitajima, C. Sato, N. Honda, T. Matsuda, M.-H. Yokoyama, B. E. Close, K. J. Colley( Role: Joint author)

    Gakushin  1999 

     More details

    Responsible for pages:69-76   Language:English

  38. KDN(=デアミノノイラミン酸)-転移酸素およびポリKDN-転移酵素の研究 : 各種KDN-転移酵素の利用による機能性KDN-複合糖質の造成と活用

    井上 康男( Role: Sole author)

    井上康男  1994 

     More details

    Language:Japanese

    CiNii Books

  39. KDN(=デアミノノイラミン酸)-転移酸素およびポリKDN-転移酵素の研究 : 各種KDN-転移酵素の利用による機能性KDN-複合糖質の造成と活用

    井上 康男( Role: Sole author)

    井上康男  1994 

     More details

    Responsible for pages:.   Language:Japanese

▼display all

MISC 21

  1. The Out of Africa and mental disorders: Adaptation with reduction in STX promoter activity

    Naoko Fujito, Yoko Satta, Masaya Hane, Atsushi Matsui, Kenta Yashima, Ken Kitajima, Chihiro Sato, Naoyuki Takahata, Toshiyuki Hayakawa

    GENES & GENETIC SYSTEMS   Vol. 91 ( 6 ) page: 343 - 343   2016.12

     More details

    Language:English   Publishing type:Research paper, summary (international conference)   Publisher:GENETICS SOC JAPAN  

    Web of Science

  2. A Uniquely Human Evolutionary Change in ST8Sia-II Impacts Enzyme Stability and Polysialic Acid Function

    Michael Vaill, Masaya Hane, Yuko Naito-Matsui, Sandra Diaz, Leela Davies, Ken Kitajima, Chihiro Sato, Ajit Varki

    GLYCOBIOLOGY   Vol. 26 ( 12 ) page: 1423 - 1423   2016.12

     More details

    Language:English   Publishing type:Research paper, summary (international conference)   Publisher:OXFORD UNIV PRESS INC  

    Web of Science

  3. Spread of reduced activity of STX promoter throughout Great Journey

    Naoko Fujito, Yoko Satta, Masaya Hane, Atsushi Matsui, Ken Kitajima, Chihiro Sato, Toshiyuki Hayakawa

    GENES & GENETIC SYSTEMS   Vol. 90 ( 6 ) page: 379 - 379   2015.12

     More details

    Language:English   Publishing type:Research paper, summary (international conference)   Publisher:GENETICS SOC JAPAN  

    Web of Science

  4. Adaptive evolution of the promoter region of the Sialyltransferase 8B (STX) gene

    Naoko T. Fujito, Toshiyuki Hayakawa, Masaya Hane, Ken Kitajima, Chihiro Sato, Yoko Satta

    GENES & GENETIC SYSTEMS   Vol. 89 ( 6 ) page: 327 - 327   2014.12

     More details

    Language:English   Publishing type:Research paper, summary (international conference)   Publisher:GENETICS SOC JAPAN  

    Web of Science

  5. The Synthesis of Sialo-containing Glycopolymers by pi-Allyl Nickel Catalyzed Coordination Polymerization

    Shuichi Ohira, Yu Yasuda, Chihiro Sato, Ken KItajima, Ikuyoshi Tomita, Takashi Takahashi, Hiroshi Tanaka

    GLYCOBIOLOGY   Vol. 24 ( 11 ) page: 1119 - 1120   2014.11

     More details

    Language:English   Publishing type:Research paper, summary (international conference)   Publisher:OXFORD UNIV PRESS INC  

    Web of Science

  6. An anti-schizophrenic drug affects the surface expression of polySia-NCAM in IMR-32 human neuroblastoma cells

    Saki Nishimura, Masaya Hane, Yuki Niimi, Ken Kitajima, Chihiro Sato

    GLYCOBIOLOGY   Vol. 24 ( 11 ) page: 1193 - 1193   2014.11

     More details

    Language:English   Publishing type:Research paper, summary (international conference)   Publisher:OXFORD UNIV PRESS INC  

    Web of Science

  7. Insight into single nucleotide polymorphisms (SNPs) of the polysialyltransferase ST8SIA2/STX in psychiatric disorders

    Masaya Hane, Saki Nishimura, Toshiyuki Hayakawa, Ken Kitajima, Chihiro Sato

    GLYCOBIOLOGY   Vol. 24 ( 11 ) page: 1206 - 1206   2014.11

     More details

    Language:English   Publishing type:Research paper, summary (international conference)   Publisher:OXFORD UNIV PRESS INC  

    Web of Science

  8. Functional analysis of tissue-specific post-translational modification of springtime hormone TSH

    IKEGAMI KEISUKE, LIAO XIAO-HUI, HOSHINO YUTA, ONO HIROKO, OTA WATARU, ITO YUKA, NISHIWAKI-OHKAWA TAEKO, SATO CHIHIRO, KITAJIMA KEN, IIGO MASAYUKI, SHIGEYOSHI YASUFUMI, YAMADA MASANOBU, MURATA YOSHIHARU, REFETOFF SAMUEL, YOSHIMURA TAKASHI

    時間生物学   Vol. 20 ( 2 ) page: 114   2014.10

     More details

    Language:English  

    J-GLOBAL

  9. ポリシアル酸転移酵素STX/ST8SIA2とPST/ST8SIA4のポリシアル酸生合成能の解析

    新美百希, 羽根正弥, 西村紗希, 宮田真路, 北島健, 佐藤ちひろ

    日本糖質学会年会要旨集   Vol. 33rd   page: 144   2014.7

     More details

    Language:Japanese  

    J-GLOBAL

  10. Polysialic acid on flagellasialin is involved in sperm motility

    Shinji Miyata, Chihiro Sato, Hironobu Kumita, Masaru Toriyama, Ken Kitajima

    ZOOLOGICAL SCIENCE   Vol. 23 ( 12 ) page: 1171 - 1171   2006.12

     More details

    Language:English   Publishing type:Research paper, summary (international conference)   Publisher:ZOOLOGICAL SOC JAPAN  

    Web of Science

  11. An N-terminal 130 kDa fragment of a fourth sea urchin sperm receptor for egg jelly module protein, REJ4, is secreted during acrosome reaction

    Shinji Miyata, Chihiro Sato, Masaru Toriyama, Ken Kitajama

    ZOOLOGICAL SCIENCE   Vol. 22 ( 12 ) page: 1456 - 1456   2005.12

     More details

    Language:English   Publishing type:Research paper, summary (international conference)   Publisher:ZOOLOGICAL SOC JAPAN  

    Web of Science

  12. Capacitation-induced changes of the surface distribution of sialoglycotopes on pig sperm

    Lian Xue, Shinji Miyata, Chihiro Sato, Ken Kitajima

    ZOOLOGICAL SCIENCE   Vol. 22 ( 12 ) page: 1456 - 1456   2005.12

     More details

    Language:English   Publishing type:Research paper, summary (international conference)   Publisher:ZOOLOGICAL SOC JAPAN  

    Web of Science

  13. ブタ精子表面に新しく見出されたシアロ糖鎖エビトープの解析

    せつ蓮, 宮田真路, 佐藤ちひろ, 北島健

    生化学   Vol. 77 ( 6 ) page: 571   2005.6

     More details

    Language:Japanese  

    J-GLOBAL

  14. The 130 kDa glycoprotein is secreted from sperm during acrosome reaction of sea urchin, Hemicentrotus pulcherrimus

    Shinji Miyata, Chihiro Sato, Masaru Toriyama, Noritaka Hirohashi, Victor Vacquier, Ken Kitajima

    ZOOLOGICAL SCIENCE   Vol. 21 ( 12 ) page: 1292 - 1292   2004.12

     More details

    Language:English   Publishing type:Research paper, summary (international conference)   Publisher:ZOOLOGICAL SOC JAPAN  

    Web of Science

  15. A major ganglioside from Hemicentrotus pulcherrimus sperm specifically interacts with phospholipids

    Nao Yamakawa, Chihiro Sato, Ken Kitajima

    ZOOLOGICAL SCIENCE   Vol. 21 ( 12 ) page: 1292 - 1292   2004.12

     More details

    Language:English   Publishing type:Research paper, summary (international conference)   Publisher:ZOOLOGICAL SOC JAPAN  

    Web of Science

  16. Development of sensitive chemical and immunochemical methods for detecting sulfated sialic acids and their application to localization and quantitation studies of sea urchin sperm and eggs

    N Yamakawa, E Maehashi, S Miyata, C Sato, K Furuhata, K Kitajima

    GLYCOBIOLOGY   Vol. 14 ( 11 ) page: 1146 - 1146   2004.11

     More details

    Language:English   Publishing type:Research paper, summary (international conference)   Publisher:OXFORD UNIV PRESS INC  

    Web of Science

  17. Occurrence and characterization of a novel sulfated alpha 2,9-linked polysialic acid-containing glycoprotein in sea urchin sperm flagellum

    S Miyata, C Sato, K Kitajima

    GLYCOBIOLOGY   Vol. 14 ( 11 ) page: 1146 - 1146   2004.11

     More details

    Language:English   Publishing type:Research paper, summary (international conference)   Publisher:OXFORD UNIV PRESS INC  

    Web of Science

  18. Mouse T cell CD166 is a disialic acid-containing glycoprotein and involved in T cell activation

    C Sato, K Nohara, T Matsuda, K Kitajima

    GLYCOBIOLOGY   Vol. 14 ( 11 ) page: 1136 - 1136   2004.11

     More details

    Language:English   Publishing type:Research paper, summary (international conference)   Publisher:OXFORD UNIV PRESS INC  

    Web of Science

  19. Mouse heat shock protein 70 recognizes negatively charged glycolipids and phospholipids

    Y Harada, C Sato, K Kitajima

    GLYCOBIOLOGY   Vol. 14 ( 11 ) page: 1101 - 1102   2004.11

     More details

    Language:English   Publishing type:Research paper, summary (international conference)   Publisher:OXFORD UNIV PRESS INC  

    Web of Science

  20. Effects of exogenously administered mannose and deaminoneuraminic acid (KDN) to mice or murine cultured cells on the metabolism of KDN

    S Go, C Sato, K Kitajima

    GLYCOBIOLOGY   Vol. 14 ( 11 ) page: 1166 - 1167   2004.11

     More details

    Language:English   Publishing type:Research paper, summary (international conference)   Publisher:OXFORD UNIV PRESS INC  

    Web of Science

  21. ウニ受精におけるシアル酸を介する相互作用と脂質ラフトの重要性

    前橋絵梨, 宮田真路, 松本晴美, 松田幹, 佐藤ちひろ, 北島健

    生化学   Vol. 74 ( 8 ) page: 777   2002.8

     More details

    Language:Japanese  

    J-GLOBAL

▼display all

Presentations 168

  1. 遺伝的及び環境要因による糖鎖発現制御から脳の健康を考える Invited

    佐藤ちひろ

    第11回グライコバイオロジクス研究会   2021.1.21  第11回グライコバイオロジクス研究会

     More details

    Event date: 2021.1

    Language:Japanese   Presentation type:Oral presentation (keynote)  

    Venue:名古屋   Country:Japan  

  2. オリゴシアル酸転移酵素ST8Sia6の発現はメラノーマを悪性化する

    畑中理菜、荒木映莉乃、羽根正弥、Wu Di、北島健、佐藤ちひろ

    第93回日本生化学会大会  2020.9.14  日本生化学会

     More details

    Event date: 2020.9

    Language:English   Presentation type:Oral presentation (general)  

    Venue:Web   Country:Japan  

  3. 環境要因としてのシアル酸投与と脳領域特異的ポリシアル酸発現の解析

    高橋佑佳、阿部智佳羅、羽根正弥、呉迪、北島 健、佐藤ちひろ

    第84回日本生化学会中部支部例会  2020.9.14  日本生化学会中部支部

     More details

    Event date: 2020.9

    Language:Japanese   Presentation type:Poster presentation  

    Venue:Web   Country:Japan  

  4. 各種脊椎動物ポリシアル酸転移酵素ST8Sia2がもたらす種特異的なpolySia-NCAM構造の解析

    村井良、羽根正弥、呉迪、北島健、佐藤ちひろ

    第93回日本生化学会大会  2020.9.14  日本生化学会

     More details

    Event date: 2020.9

    Language:Japanese   Presentation type:Poster presentation  

    Venue:Web   Country:Japan  

  5. ラクタム化シアル酸誘導体の合成とその分解反応に関する研究.

    大野ひろみ、安藤弘宗、川上信彦、岩田章子、佐藤ちひろ、北島健、石田秀治、木曽真

    日本農芸化学会2009年度大会 

     More details

    Event date: 2009.3

    Language:Japanese   Presentation type:Poster presentation  

    Country:Japan  

  6. 魚類CMP-シアル酸合成酵素の構造と性質

    藤田明子、安川裕子、佐藤ちひろ、北島健

    日本農芸化学会2009年度大会 

     More details

    Event date: 2009.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  7. ポリシアル酸と脳由来神経栄養因子の相互作用の解析

    金戸幸弘、北島健、佐藤ちひろ

    日本農芸化学会2009年度大会 

     More details

    Event date: 2009.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  8. ポリシアル酸とFGF-2の相互作用の解析.

    小野明日香、金戸幸弘、北島 健、佐藤ちひろ

    日本農芸化学会2009年度大会 

     More details

    Event date: 2009.3

    Language:Japanese   Presentation type:Poster presentation  

    Country:Japan  

  9. Polysialic acid is not a negative regulator any longer in neurogenesis and neuronal functions: A new finding of its binding to a particular group of neurotransmitters. International conference

    Ken Kitajima, Ryo Isomura, and Chihiro Sato

    Gordon Research Conference on Glycobiology,  

     More details

    Event date: 2009.1

    Language:English   Presentation type:Poster presentation  

    Country:United States  

  10. 昆虫細胞由来シアル酸9-リン酸合成酵素の構造と基質特異性の解析

    濱口香代、郷慎司、佐藤ちひろ、小林迪弘、池田素子、北島健

    第81回日本生化学会大会 

     More details

    Event date: 2008.12

    Language:Japanese   Presentation type:Poster presentation  

    Country:Japan  

  11. 抗ジシアル酸抗体が認識する急性期タンパク質の同定. International conference

    安川然太、北島健、佐藤ちひろ

    第81回日本生化学会大会 

     More details

    Event date: 2008.12

    Language:English   Presentation type:Poster presentation  

    Country:Japan  

  12. 化学的検出法を用いた天然におけるサイクリックシアル酸の局在検索.

    岩田章子、佐藤ちひろ、安藤弘宗、石田秀治、木曽真、神奈木玲児、北島健

    第81回日本生化学会大会 

     More details

    Event date: 2008.12

    Language:Japanese   Presentation type:Poster presentation  

    Country:Japan  

  13. ポリシアル酸は特定の神経伝達物質と相互作用する. International conference

    磯村遼、北島健、佐藤ちひろ

    第81回日本生化学会大会 

     More details

    Event date: 2008.12

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  14. mAb.A2B5の抗原特異性の決定とマウス脳発達段階におけるmAb.A2B5反応性オリゴシアル酸含有糖タンパク質の発現解析 International conference

    猪子恵未、西浦祐二、田中浩士、高橋孝志、北島健、佐藤ちひろ

    第81回日本生化学会大会 

     More details

    Event date: 2008.12

    Language:English   Presentation type:Poster presentation  

    Country:Japan  

  15. 酸性多糖と神経栄養因子との相互作用解析. International conference

    金戸幸弘、北島健、佐藤ちひろ

    第81回日本生化学会大会 

     More details

    Event date: 2008.12

    Language:English   Presentation type:Oral presentation (general)  

    Country:Japan  

  16. 哺乳類シアロ糖タンパク質のデアミノノイラミン酸 (KDN) 修飾糖タンパク質への改変.

    中島優子、藤田明子、佐藤ちひろ、北島健

    第81回日本生化学会大会 

     More details

    Event date: 2008.12

    Language:Japanese   Presentation type:Poster presentation  

    Country:Japan  

  17. ポリシアル酸の新機能

    佐藤ちひろ

    第6回日本糖鎖科学コンソーシアムシンポジウム-糖鎖研究と他領域との統合- 

     More details

    Event date: 2008.12

    Language:Japanese   Presentation type:Symposium, workshop panel (nominated)  

    Country:Japan  

  18. ウニ配偶子生体膜マイクロドメイン間接着に関わるガングリオシド-中性糖脂質相互作用の発見.

    山川奈緒、佐藤ちひろ、北島健

    第81回日本生化学会大会 

     More details

    Event date: 2008.12

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  19. Novel insights into the novel binding sites of the sialic acid biding Ig-like lectin-7 (Siglec-7) gained by in silico docking studies. International conference

    A one-day symposium on Sialyltransferases, Functional Glycomics, and Genomics of Zebrafish, 

     More details

    Event date: 2008.11

    Language:English   Presentation type:Symposium, workshop panel (nominated)  

    Country:Taiwan, Province of China  

  20. Membrane microdomains from early gastrula embryos of medaka are a platform of E-cadherin- and LeX-mediated cell-cell interactions during epiboly. 14th

    Japanese Medaka and Zebrafish Meeting 

     More details

    Event date: 2008.9

    Language:English   Presentation type:Oral presentation (general)  

    Country:Japan  

  21. CMP-[14C]デアミノノイラミン酸(KDN)のワンポット酵素合成法の確立

    中島優子、藤田明子、佐藤ちひろ、北島健

    糖鎖科学名古屋拠点「若手の力」フォーラム第6回 

     More details

    Event date: 2008.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  22. 酸性多糖と神経栄養因子との相互作用解析

    金戸幸広、北島 健、佐藤ちひろ

    糖鎖科学名古屋拠点「若手の力」フォーラム第6回 

     More details

    Event date: 2008.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  23. 鱗翅目昆虫細胞におけるシアル酸9-リン酸合成酵素ホモログの同定.

    濱口香代、郷慎司、佐藤ちひろ、小林迪弘、池田素子、北島健

    糖鎖科学名古屋拠点「若手の力」フォーラム第6回 

     More details

    Event date: 2008.8

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  24. ポリシアル酸の神経伝達分子結合活性の発見と性質

    磯村遼、北島健、佐藤ちひろ

    FCCAグライコサイエンス若手フォーラム2008 

     More details

    Event date: 2008.8

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  25. CMP-シアル酸合成酵素の基質認識および細胞内局在の分子機構の解明

    藤田明子, 佐藤ちひろ, Tiralongo, Joe, Münster-Kühnel, Anja K., Gerardy-Schahn, Rita, 北島健

    第28回日本糖質学会年会 

     More details

    Event date: 2008.8

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  26. 抗ガングリオシド抗体A2B5が認識する糖タンパク質のマウス新生児脳における発現.

    猪子恵未、西浦祐二、田中浩士、高橋孝志、北島健、佐藤ちひろ

    FCCAグライコサイエンス若手フォーラム2008 

     More details

    Event date: 2008.8

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  27. ウニ卵膜マイクロドメインに対する単クローン抗体の認識エピトープ解析.

    中村奈央子、前橋絵梨、山川奈緒、佐藤ちひろ、鳥山優、北島健

     FCCAグライコサイエンス若手フォーラム2008 

     More details

    Event date: 2008.8

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  28. Complex formation between polysialic acid and brain derived neurotrophic factor (BDNF). International conference

    Chihiro Sato, Yukihiro Kanato, and Ken Kitajima:

    24th International Carbohydrate Symposium, 

     More details

    Event date: 2008.7 - 2008.8

    Language:English   Presentation type:Poster presentation  

  29. In vitro and in silico docking studies of heat shock protein (Hsp) 70 with sulfatide. International conference

    Nao Yamakawa, Yoichiro Harada, Chihiro Sato, Gerard Vergoten, Ken Kitajima

    24th International Carbohydrate Symposium, 

     More details

    Event date: 2008.7 - 2008.8

    Language:English   Presentation type:Poster presentation  

  30. Brain-derived neurotrophic factor binds to polysialic acid directly depending on degree of polymerization. International conference

    Yukihiro Kanato, Ken Kitajima, Chihiro Sato

    SialoGlyco Conference 2008 

     More details

    Event date: 2008.7

    Language:English   Presentation type:Oral presentation (invited, special)  

  31. New Findings on the Expression of Non-human Sialic Acid Neu5Gc, and KDN in Cultured Human Cells. International conference

    SialoGlyco Conference 2008 

     More details

    Event date: 2008.7

    Language:English   Presentation type:Oral presentation (invited, special)  

  32. Occurrence of the Cyclic Sialic Acid in Mammalian Cells and Tissues As Demonstrated by Chemical Methods. International conference

    K. Kitajima, S.Iwata, N. Kawakami, C. Sato, H. Ando, H. Ohno, H. Ishida, M. Kiso, O. Taguchi, R. Kannagi:

    SialoGlyco Conference 2008 

     More details

    Event date: 2008.7

    Language:English   Presentation type:Oral presentation (invited, special)  

  33. One-pot Enzymatic Synthesis of CMP-[14C]deaminoneuraminic Acid (KDN). International conference

    Yuko Nakajima, Akiko Fujita, Chihiro Sato, and Ken Kitajima

    SialoGlyco Conference 2008 

     More details

    Event date: 2008.7

    Language:English   Presentation type:Poster presentation  

  34. In silico binding studies predict novel sialic acid binding sites on the sialic acid binding Ig-like lectin-7 (Siglec-7) International conference

    Nao Yamakawa, Chihiro Sato, Paul R. Crocker, Gérard Vergoten, and Ken Kitajima: I

    SialoGlyco Conference 2008 

     More details

    Event date: 2008.7

    Language:English   Presentation type:Poster presentation  

  35. Identification of the nuclear export signals that regulate the intracellular localization of the mouse CMP-sialic acid synthetase. International conference

    Akiko Fujita, Chihiro Sato, and Ken Kitajima

    SialoGlyco Conference 2008 

     More details

    Event date: 2008.7

    Language:English   Presentation type:Oral presentation (invited, special)  

  36. CMP-デアミノノイラミン酸(CMP-KDN)のワンポット酵素合成法の確立

    日本農芸化学会2008年度大会 

     More details

    Event date: 2008.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  37. 抗ジシアル酸が認識するマウス血清タンパク質の同定

    安川然太、北島健、佐藤ちひろ

    日本農芸化学会2008年度大会 

     More details

    Event date: 2008.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  38. 分子モデリング法による熱ショック蛋白質の酸性糖脂質結合性の解析

    山川奈緒、原田陽一郎、佐藤ちひろ、Gerard Vergoten、北島健

    日本農芸化学会2008年度大会 

     More details

    Event date: 2008.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  39. マウスCMP-シアル酸合成酵素の核外移行シグナル (NES) の同定

    藤田明子、佐藤ちひろ、北島 健

     More details

    Event date: 2008.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  40. 脊椎動物CMP-シアル酸合成酵素の基質認識および細胞内局在の分子機構の解明.

    藤田明子, 佐藤ちひろ, Tiralongo, Joe, Münster-Kühnel, Anja K., Gerardy-Schahn, Rita, 北島健

    第28回日本糖質学会年会 

     More details

    Event date: 2007.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  41. 分子シミュレーションによる熱ショックタンパク質の酸性糖脂質結合性の解析

    山川 奈緒、原田陽一郎、佐藤ちひろ、Gerard Vergoten, 北島 健

     More details

    Event date: 2007

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  42. Short-term regulation of polysialic acid expression in microglia

    Gordon Research Conference on Glycobiology 

     More details

    Event date: 2007

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

  43. A rapid clearance of polysialic acid on microglia by inflammatory stimulation International conference

    3rd International Meeting on polysialic acid 

     More details

    Event date: 2007

    Language:English   Presentation type:Poster presentation  

  44. シアル酸重合体の生物学的意義の解明

    佐藤ちひろ、矢部宇一郎、安川然太1,2、宮田真路、澤田 誠、北島 健

    文科省特定領域研究 糖鎖によるタンパク質と分子複合体の機能調節 第5回公開シンポジウム 

     More details

    Event date: 2007

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  45. ジシアリル化修飾をうけた血清糖タンパク質の同定

    安川然太、佐野琴音、小川温子、北島 健、佐藤ちひろ

    日本農芸化学会2007年度大会 

     More details

    Event date: 2007

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  46. 脊椎動物由来CMP-シアル酸合成酵素によるデアミノノイラミン酸(KDN)の認識機構

    藤田 明子、佐藤ちひろ、北島 健

    日本農芸化学会2007年度大会 

     More details

    Event date: 2007

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  47. サイクリックシアル酸グリコシドの化学的性質の解析

    岩田章子、川上信彦、佐藤ちひろ、安藤弘宗、木曽 真、神奈木玲児、北島 健

    日本農芸化学会2007年度大会 

     More details

    Event date: 2007

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  48. 酸性糖脂質による70kDa熱ショックタンパク質の複合体形成

    原田陽一郎、佐藤ちひろ、北島 健

    日本農芸化学会2007年度大会 

     More details

    Event date: 2007

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  49. 70kDa-熱ショックタンパク質の酸性糖脂質誘導性の高分子複合体形成能の発見

    原田陽一郎、佐藤ちひろ、北島 健

    第27回 日本糖質学会年会 

     More details

    Event date: 2007

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  50. サイクリックシアル酸グリコシドの酸加水分解産物の同定

    川上信彦、岩田章子、佐藤ちひろ、安藤弘宗、木曽 真、神奈木玲児、北島健

    糖鎖科学名古屋拠点 若手の力フォーラム 第5回 

     More details

    Event date: 2007

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  51. O-型糖鎖上のポリシアル酸の生合成機構の解明

    朝比奈慎二、佐藤ちひろ、松野みどり、松田 幹、Karen, J. Colley, 北島 健

    糖鎖科学名古屋拠点 若手の力フォーラム 第4回 

     More details

    Event date: 2006

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  52. メダカ初期胚における糖鎖抗原3D11の発現と役割

    北島 健、原 弘明、足立朋子、佐藤ちひろ:

    第77回日本動物学会大会 

     More details

    Event date: 2006

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  53. ポリシアル酸の探求と活用

    佐藤ちひろ

    第5回 糖鎖分科会 

     More details

    Event date: 2006

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  54. ミクログリア細胞におけるポリシアル酸構造の発現.

    佐藤ちひろ

    文科省特定領域研究 糖鎖によるタンパク質と分子複合体の機能調節 第四回公開シンポジウム 

     More details

    Event date: 2006

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  55. Hsp70のN型糖鎖結合活性の証明とそのシャペロン活性における役割.

    原田陽一郎、佐藤ちひろ、北島健:

    第7回関西グライコサイエンスフォーラム 

     More details

    Event date: 2006

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  56. 哺乳類細胞におけるデアミノノイラミン酸(KDN)代謝調節機構の解析

    郷 慎司、佐藤ちひろ、北島 健

    第26回 日本糖質学会年会 

     More details

    Event date: 2006

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  57. ウニ精子鞭毛局在flagellasialinのユニークな糖鎖修飾の精子運動性における重要性

    宮田真路, 佐藤ちひろ, 久美田紘信, 鳥山 優, 北島 健

    第77回日本動物学会大会 

     More details

    Event date: 2006

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  58. Polysialic acid on microglia cell and its clearance after LPS-induced activation.

    Sialoglycoscience 2006 

     More details

    Event date: 2006

    Language:English   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  59. Discovery of polysialic acid in microglia. Expression changes of α2,8-polysialyltransferses during activation of a mouse microglia cell line. International conference

    5th International Symposium on Glycosyltransferases 

     More details

    Event date: 2006

    Language:English   Presentation type:Poster presentation  

    Country:Japan  

  60. ミクログリア細胞におけるポリシアル酸構造の存在.

    矢部宇一郎、北島 健、霜田 靖、渡邊 和忠 澤田 誠、佐藤ちひろ

    第78回日本生化学会大会 

     More details

    Event date: 2005

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  61. An essential role of carbohydrate-enriched membrane microdomains (rafts) in gastrulation of medaka fish, Oryzias latipes. International conference

    Gordon Research Conference on Glycobiology 

     More details

    Event date: 2005

    Language:English   Presentation type:Oral presentation (invited, special)  

  62. Milk CD36 Hannah Research Institute International conference

    Nagoya University Joint Workshop on Animal Biotechnology 

     More details

    Event date: 2005

    Language:English   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  63. 糖タンパク質の多様なポリシアル酸修飾の意義を探る.

    佐藤ちひろ

    文科省特定領域研究 糖鎖によるタンパク質と分子複合体の機能調節 第三回夏期シンポジウム 

     More details

    Event date: 2005

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  64. Significance of membrane microdomains in the carbohydrate-dependent cell-cell interactions during early development of medaka fish.

    The 2nd Japan-The Netherlands Glycobiology Symposium. 

     More details

    Event date: 2005

    Language:English   Presentation type:Oral presentation (invited, special)  

  65. Biosynthesis and subcellular localiztion of deaminoneuraminic acid (KDN) in mammalian cells. International conference

    The 18th International Symposium on Glycoconjugates. 

     More details

    Event date: 2005

    Language:English   Presentation type:Poster presentation  

  66. Demonstration of N-glycan-binding activity of HSP70 and its importance in the chaperoning activity.

    The 18th International Symposium on Glycoconjugates. 

     More details

    Event date: 2005

    Language:English   Presentation type:Poster presentation  

  67. Development of a chemical method for detecting a unique sialic acid, cyclic neuraminic acid, in naturally-occurring sialyl glycoconjugates. International conference

    Pacifichem 

     More details

    Event date: 2005

    Language:English   Presentation type:Oral presentation (invited, special)  

  68. マウス乳糖タンパク質CD36上のポリシアル酸の乳腺発達段階における発現解析

    矢部宇一郎、佐藤ちひろ、北島 健

    日本農芸化学会2005年度大会 

     More details

    Event date: 2005

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  69. 新規ポリシアル酸含有糖タンパク質flagellasialinの発見、構造および機能.

    宮田真路, 佐藤ちひろ, 久美田紘信, 鳥山 優, Victor D. Vacquier, 北島 健

    第25回日本糖質学会 

     More details

    Event date: 2005

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  70. ジ・オリゴシアル酸を含有するマウス血清糖タンパク質の同定

    安川然太、佐藤ちひろ、北島 健

    第3回糖鎖科学名古屋拠点 若手の力 

     More details

    Event date: 2005

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  71. メダカ初期胚における細胞膜ラフト形成の重要性とその役割.

    足立朋子、佐藤ちひろ、北島 健

    第3回糖鎖科学名古屋拠点 若手の力 

     More details

    Event date: 2005

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  72. Chain Length Diversity of Sialic Acids and Its Biological Significance

    SATO Chihiro

    Trends in Glycoscience and Glycotechnology 

     More details

    Event date: 2004.9

    Language:English   Presentation type:Oral presentation (general)  

    Sialic acids are commonly present as monosialyl residues at the non-reducing terminal end of glycoconjugates. In some cases, &alpha;2&rarr;8 linked di/oligosialic acid chains with DP 2 or 3 sialic acid residues are common structural units of gangliosides, and involved in various biological processes, such as cell adhesion, cell differentiation, signal transduction, and surface expression of stage specific antigen. In contrast, little attention has been paid to the occurrence and functions of such short sialyl di/oligomers on glycoproteins, while polysialic acid (DP&ge;8) in embryonic NCAM has been extensively studied as a regulator of cell adhesion in neurogenesis. As analytical methods to detect di/oligosialic acid structures have been improved, several glycoproteins containing di/oligo/polysialic acid chains have been identified. It is thus hypothesized that these di/oligosialic acid residues on glycoproteins may have similar important functions in common with those proposed for the gangliosides or may have new functions. Currently, several studies showing the importance of di/oligosialic acid-containing glycoproteins have emerged. In this review, recent advances in such studies of di/oligosialic acid residues on glycoproteins, including analytical methods, occurrence, biosynthetic pathways, and functions, are described.

    DOI: 10.4052/tigg.16.331

    Scopus

    CiNii Article

  73. Preface for the Special Issue Entitled &ldquo;New Waves in Sialobioscience&rdquo;

    Sato Chihiro

    Trends in Glycoscience and Glycotechnology 

     More details

    Event date: 2004.9

    Language:English   Presentation type:Oral presentation (general)  

    DOI: 10.4052/tigg.16.291

    Scopus

    CiNii Article

  74. Occurrence of a novel 8-O-sulfated Neu5Ac-capped a2,8-linked polyNeu5Ac chains in the sea urchin sperm flagella glycoprotein

     More details

    Event date: 2004

    Language:Japanese   Presentation type:Poster presentation  

    Country:Japan  

  75. Expression of the disialic acid structure on glycoproteins during T cell activation International conference

    Sialobiology 2004 

     More details

    Event date: 2004

    Language:English   Presentation type:Poster presentation  

  76. Identification and characterization of a new heat shock protein-like lectin on the egg surface of sea urchin International conference

    21st International Lectin Meeting 

     More details

    Event date: 2004

    Language:English   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  77. 動物細胞におけるシアル酸の取り込み、分解に関する研究

    郷慎司、佐藤ちひろ、北島健

    日本農芸化学会2004年度大会 

     More details

    Event date: 2004

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  78. ウニ卵局在精子結合タンパク質 (SBP)のレクチン活性

    原田陽一郎、佐藤ちひろ、北島健

    日本農芸化学会2004年度大会 

     More details

    Event date: 2004

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  79. 鳥類卵膜マトリクスの in vitro での再形成:糖タンパク質ZP1とZPCの特異的会合

    奥村裕紀、青木直人、佐藤ちひろ、灘野大太、松田幹

    日本農芸化学会2004年度大会 

     More details

    Event date: 2004

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  80. 食物抗原の腸管吸収:吸収された卵アルブミンの末梢血中での量的変動と質的変化

    松原 毅、青木直人、佐藤ちひろ、灘野大太、松田 幹

    日本農芸化学会2004年度大会 

     More details

    Event date: 2004

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  81. 膜リン脂質結合タンパク質MFG-E8の退縮期乳腺での発現変動

    中谷 肇、青山康二、青木直人、佐藤ちひろ、灘野大太、松田 幹

    日本農芸化学会2004年度大会 

     More details

    Event date: 2004

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  82. Study on the uptake of deaminoneuraminic acid (KDN) by mammalian cells

     More details

    Event date: 2004

    Language:Japanese   Presentation type:Poster presentation  

    Country:Japan  

  83. Glycolipid- and phospholipid-binding activity of heat shock protein 70

     More details

    Event date: 2004

    Language:Japanese   Presentation type:Poster presentation  

    Country:Japan  

  84. Roles of carbohydrate-mediated lipid raft-lipid raft interactions in early development of medaka, Oryzias latipes

     More details

    Event date: 2003

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  85. 新規糖アミノ酸α-C-マンノシルトリプトファンの合成と生物機能解析

    西川俊夫、梶井重男、和田享子、石川 幸、磯部 稔、佐藤ちひろ、安川然太、北島 健

    第24回日本糖質学会年会; 

     More details

    Event date: 2003

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  86. ウニ精子鞭毛における新規ポリシアル酸含有糖タンパク質の発見とその糖鎖構造解析

    宮田真路, 佐藤ちひろ, 鳥山 優、北島 健

    第74回日本動物学会大会 

     More details

    Event date: 2003

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  87. メダカ初期胚発生における細胞膜マイクロドメイン形成の重要性の検討

    足立朋子、佐藤ちひろ、北島 健

     More details

    Event date: 2003

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  88. Characterization of a disialic acid-containing glycoprotein expressed on neural cells

     More details

    Event date: 2003

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  89. ヒトミルクにおける新しいポリシアル酸含有糖タンパク質CD36の発見

    矢部宇一郎, 佐藤ちひろ, 松田 幹, 北島 健

    第24回日本糖質学会年会 

     More details

    Event date: 2003

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  90. ジおよびオリゴシアル酸含有糖タンパク質の構造と機能

    佐藤ちひろ

    糖鎖科学名古屋拠点第1回「若手の力」フォーラム 

     More details

    Event date: 2003

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  91. シアル酸の重合度多様性の発見とその生物学的意義の解明

    佐藤ちひろ

    第24回日本糖質学会年会(奨励賞講演) 

     More details

    Event date: 2003

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  92. Discovery of two new polysialoglycoproteins in human and sea urchin. Linkage and length diversities of polysialic acid in animal glycoproteins. International conference

    Annual Conference of the Society for Glycobiology 

     More details

    Event date: 2003

    Language:English   Presentation type:Oral presentation (invited, special)  

  93. ヒトおよびマウスのミルクにオリゴ・ポリシアル酸構造が存在した

    矢部 宇一郎、佐藤ちひろ、松田 幹、北島 健

    日本農芸化学会2003年度大会 

     More details

    Event date: 2003

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  94. シアル酸合成能をもつ昆虫細胞の検索とその存在証明

    郷 慎司、佐藤ちひろ、池田素子、小林迪弘、松田 幹、北島 健

    日本農芸化学会2003年度大会; 

     More details

    Event date: 2003

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  95. ジシアル酸含有糖タンパク質の神経細胞(Neuro2A)における発現

    佐藤ちひろ、松田 幹、北島 健

    第75回日本生化学会年会 

     More details

    Event date: 2002

    Language:Japanese   Presentation type:Poster presentation  

    Country:Japan  

  96. 精子膜マイクロドメインに局在する糖脂質と糖タンパク質に共通の糖鎖エピトープの機能

    宮田真路、前橋絵梨、太田薫、佐藤ちひろ、松田幹、北島 健

    第73回 日本動物学会年会 

     More details

    Event date: 2002

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  97. メダカ初期胚における細胞膜ドメイン(ラフト)の単離と細胞間相互作用における機能

    足立朋子、佐藤ちひろ、松田 幹、戸谷一英、村田健臣、碓氷泰一、北島 健

    第73回 日本動物学会年会 

     More details

    Event date: 2002

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  98. Sialic acid-mediated interactions at sea urchin International conference

    The Third Pan-Pacific Conference on Sialoglycoscience and Other Novel Forms of Glycosylation 

     More details

    Event date: 2002

    Language:English   Presentation type:Oral presentation (invited, special)  

  99. マンノース経口投与マウスの臓器におけるKDN合成

    郷 慎司、仲田大輔、佐藤ちひろ、松田 幹、北島 健

    日本農芸化学会2002年度大会 

     More details

    Event date: 2002

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  100. ニジマスポリシアル酸転移酵素(PST)のcDNAクローニングと発現解析

    朝比奈慎二、佐藤ちひろ、松田 幹、北島 健

    日本農芸化学会2002年度大会 

     More details

    Event date: 2002

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  101. ニジマス卵巣におけるO-型糖鎖上のポリシアル酸構造の生合成に関与するシアル酸転移酵素のクローニング

    朝比奈慎二、松野みどり、佐藤ちひろ、北島 健

    第23回日本糖質学会年会 

     More details

    Event date: 2002

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  102. Sialic acid-dependent raft-raft interactions at fertilization International conference

    XVIth International Symposium on glycoconjugates 

     More details

    Event date: 2001.8

    Language:English   Presentation type:Oral presentation (invited, special)  

  103. Identification of a new diSia-containing glycoprotein (adipoQ) in bovine and mouse sera International conference

    XVIth International Symposium on Glycoconjugates 

     More details

    Event date: 2001.7

    Language:English   Presentation type:Poster presentation  

    Congress Center The Hague Netherlands

  104. 哺乳動物に見いだされるオリゴ・ポリシアル酸含有糖タンパク質ファミリーの構造と機能

    佐藤ちひろ

    第43回FCCAセミナー 

     More details

    Event date: 2001.7

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    "グランシップ, 静岡"

  105. "Identification of adipo Q, an adipocyte-specific glycoprotein, as a new oligosialic acid-containing glycoprotein and a possible substrate for the a2,8-sialyltransferase III (ST8SiaIII)." International conference

    "International Symposium on Protein Traffic, Glycosylation, and Human Health" 

     More details

    Event date: 2001.5

    Language:English   Presentation type:Poster presentation  

  106. 精子ラフト-卵ラフト間相互作用の存在とその受精における重要性

    北島 健、前橋絵梨、太田 薫、佐藤ちひろ、松田 幹、鳥山 優

    日本動物学会第72回大会 

     More details

    Event date: 2001

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  107. ニジマス卵巣&#61537;2,6-シアル酸転移酵素のcDNAクローニングと組み換え体酵素の   性質

    朝比奈慎二、佐藤ちひろ、松田 幹、北島 健

    2001年度農芸化学大会 

     More details

    Event date: 2001

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  108. 血清糖タンパク質におけるジシアル酸およびオリゴシアル酸残基の 同定および生合成

    佐藤ちひろ、安川然太、本田直輝、松田 幹、北島 健

    第22回日本糖質学会年会 

     More details

    Event date: 2001

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  109. Roles of Membrane Microdomains (Rafts) in Sperm-Egg Interaction. International conference

    Second Canada-Japan Bilateral Workshop on Human Reproduction and Reproductive Biology 

     More details

    Event date: 2001

    Language:English   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  110. 糖タンパク質糖鎖におけるジおよびオリゴシアル酸の構造と機能

    佐藤ちひろ

    糖鎖物質設計シンポジウム 

     More details

    Event date: 2000.8

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  111. "Expression and glycosylation of a major, carbohydrate-rich, cortical alveolus glycoprotein (hyosophorin) during oogenesis of medaka fish, Oryzias latipes" International conference

    3rd International Symposium of the Molecular and Cellular Biology of the Egg and Embryo 

     More details

    Event date: 2000.8

    Language:English   Presentation type:Poster presentation  

  112. Di- and oligosialic acids as novel carbohydrate epitopes in leukocytes International conference

    7th Conference on human leukocyte differentiation antigens 

     More details

    Event date: 2000.6

    Language:English   Presentation type:Poster presentation  

  113. Membrane microdomains are involved in carbohydrate-dependent sperm-egg interaction in sea urchin International conference

    3rd International Symposium of the Molecular and Cellular Biology of the Egg and Embryo 

     More details

    Event date: 2000.1

    Language:English   Presentation type:Oral presentation (invited, special)  

  114. 脂肪細胞の分化に伴うジおよびオリゴシアル酸含有糖タンパク質とα2,8-シアル酸 転移酵素の発現変化

    佐藤ちひろ、松田 幹、北島 健

    第73回日本生化学会大会 

     More details

    Event date: 2000

    Language:Japanese   Presentation type:Poster presentation  

    Country:Japan  

  115. Lipid raft on gametic cells as a functional domain for sperm-egg interaction coupled with signal transduction International conference

    The International Symposium on Development and Fertilization of Sea Urchin and Marine Invertebrates 

     More details

    Event date: 1999.12

    Language:English   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  116. Egg receptor for sperm binds to gangliosides in the low density detergent-insoluble membrane (LD-DIM) domain of the sperm surface. Possible involvement of the LD-DIM in sperm-egg binding coupled with signal transduction during fertilization. International conference

    XVth International Symposium on Glycoconjugates 

     More details

    Event date: 1999.8

    Language:English   Presentation type:Poster presentation  

    Country:Japan  

  117. Glycosphingolipid enriched microdomain on gametic cells as functional domain for sperm-egg interaction coupled with signal transduction International conference

    Gordon Research Conference on Fertilization and Activation of the Development 

     More details

    Event date: 1999.6

    Language:English   Presentation type:Oral presentation (invited, special)  

  118. 哺乳動物におけるオリゴシアル酸含有糖タンパク質の普遍的存在および分化に伴う発現変化

    佐藤ちひろ、松田 幹、北島 健

    第72回日本生化学会大会 

     More details

    Event date: 1999

    Language:Japanese   Presentation type:Poster presentation  

    Country:Japan  

  119. オリゴシアル酸含有糖タンパク質の新しい化学的および免疫化学的解析法の開発と哺乳動物細胞における普遍的存在の証明

    "佐藤ちひろ,本田直輝,太田薫,福岡秀幸,井上貞子,松田幹,北島健"

    第71回日本生化学会大会 

     More details

    Event date: 1998.10

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  120. "Expression of a2,8-linked oligosialic acid-containing glycoproteins in mammalian cells, depending on development, cell differentiation, and overexpression of polysialyltransferase genes." International conference

    XIXth International Carbohydrate Symposium 

     More details

    Event date: 1998.8

    Language:English   Presentation type:Oral presentation (invited, special)  

  121. Frequent occurrence of oligosialyl units in vertebrate glycoproteins as evidenced by new chemical and immunochemical methods. International conference

    XIVth International Symposium on Glycoconjugates 

     More details

    Event date: 1997.9

    Language:English   Presentation type:Oral presentation (invited, special)  

  122. ブタ脳に存在するオリゴN-アセチルノイラミン酸含有糖タンパク質

    佐藤ちひろ、福岡秀幸、松田幹、井上貞子、井上康男、北島 健

    第70回日本生化学会大会 

     More details

    Event date: 1997

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  123. 哺乳動物細胞におけるオリゴ/ポリN-グリコリルノイラミン酸含有糖タンパク質の存在証明

    佐藤ちひろ、北島 健、古川清、井上貞子、井上康男

    第19回糖質シンポジウム 

     More details

    Event date: 1997

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  124. オリゴシアリル化は哺乳動物組織における糖タンパク質糖鎖の普遍的修飾反応である

    北島 健、佐藤ちひろ、福岡秀幸、松田幹、越野理香、小林一清、井上貞子、井上康男

    第19回糖質シンポジウム 

     More details

    Event date: 1997

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  125. 哺乳動物におけるKDN-およびpoly-α2,8-KDN含有複合糖質の免疫組織化学的および化学・生化学的同定とその生物学的意義

    井上貞子、北島 健、金森審子、佐藤ちひろ、Marti Ziak、Baoxi Qu、Xulei Zuo、Christian Zuber、Jurgen Roth、井上康男

    第18回糖質シンポジウム 

     More details

    Event date: 1996

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  126. α2→8-結合N-グリコリルノイラミン酸(Neu5Gc)オリゴマー構造を特異的に認識する単クローン抗体の調製と特異性の決定

    佐藤ちひろ、北島 健、井上康男、井上貞子

    第68回日本生化学会大会 

     More details

    Event date: 1995

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  127. Unique structures and structural chains of the cortical alveolar-derived glycopolyproteins in fish eggs upon fertilization and during early development. International conference

    3rd International Marine Biotechnology Conference 

     More details

    Event date: 1994

    Language:English   Presentation type:Oral presentation (invited, special)  

  128. 合成ポリシアロ糖脂質を用いたポリシアル酸微量検出法の開発

    佐藤ちひろ、北島 健、井上康男、井上貞子

    第66回日本生化学会大会 

     More details

    Event date: 1993

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  129. Diversity of polysialic acids structure in polysialoglycoproteins of Salmonidae fish eggs: Occurrence of α2→8-linked Poly(Neu5Ac), Poly(Neu5Gc), Poly(KDN), Poly(Neu5Ac,Neu5Gc), and their partially O-acetylated derivatives. International conference

    International Symposium on Polysialic Acid 

     More details

    Event date: 1992.8

    Language:English   Presentation type:Oral presentation (invited, special)  

  130. Polysialic acids in animals. International conference

    6th the Federation of Asian and Oceanian Biochemists Congress 

     More details

    Event date: 1992

    Language:English   Presentation type:Oral presentation (invited, special)  

  131. ポリシアロ糖タンパク質における α2→8 結合ポリシアル酸構造の多様性

    佐藤ちひろ、北島 健、田沢一朗、井上康男、井上貞子

    第65回日本生化学会大会 

     More details

    Event date: 1992

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  132. ポリシアル酸とFGF-2の相互作用の解析. International conference

    小野明日香, 金戸幸弘, 北島 健, 佐藤ちひろ

    日本農芸化学会2009年度大会  2009.3.29 

     More details

    Language:Japanese   Presentation type:Poster presentation  

  133. 魚類CMP-シアル酸合成酵素の構造と性質 International conference

    藤田明子, 安川裕子, 佐藤ちひろ, 北島健

    日本農芸化学会2009年度大会  2009.3.29 

     More details

    Language:Japanese   Presentation type:Oral presentation (general)  

  134. ラクタム化シアル酸誘導体の合成とその分解反応に関する研究. International conference

    大野ひろみ, 安藤弘宗, 川上信彦, 岩田章子, 佐藤ちひろ, 北島健, 石田秀治, 木曽真

    日本農芸化学会2009年度大会  2009.3.29 

     More details

    Language:Japanese   Presentation type:Poster presentation  

  135. ポリシアル酸と脳由来神経栄養因子の相互作用の解析 International conference

    金戸幸弘, 北島健, 佐藤ちひろ

    日本農芸化学会2009年度大会  2009.3.29 

     More details

    Language:Japanese   Presentation type:Oral presentation (general)  

  136. Polysialic acid is not a negative regulator any longer in neurogenesis and neuronal functions: A new finding of its binding to a particular group of neurotransmitters.

    Ken Kitajima, Ryo Isomura, Chihiro Sato

    Gordon Research Conference on Glycobiology,  2009.1.18 

     More details

    Language:English   Presentation type:Poster presentation  

  137. ポリシアル酸は特定の神経伝達物質と相互作用する.

    磯村遼, 北島健, 佐藤ちひろ

    第81回日本生化学会大会  2008.12.9 

     More details

    Language:Japanese   Presentation type:Oral presentation (general)  

  138. mAb.A2B5の抗原特異性の決定とマウス脳発達段階におけるmAb.A2B5反応性オリゴシアル酸含有糖タンパク質の発現解析

    猪子恵未, 西浦祐二, 田中浩士, 高橋孝志, 北島健, 佐藤ちひろ

    第81回日本生化学会大会  2008.12.9 

     More details

    Language:English   Presentation type:Poster presentation  

  139. 酸性多糖と神経栄養因子との相互作用解析.

    金戸幸弘, 北島健, 佐藤ちひろ

    第81回日本生化学会大会  2008.12.9 

     More details

    Language:English   Presentation type:Oral presentation (general)  

  140. 昆虫細胞由来シアル酸9-リン酸合成酵素の構造と基質特異性の解析 International conference

    濱口香代, 郷慎司, 佐藤ちひろ, 小林迪弘, 池田素子, 北島健

    第81回日本生化学会大会  2008.12.9 

     More details

    Language:Japanese   Presentation type:Poster presentation  

  141. 抗ジシアル酸抗体が認識する急性期タンパク質の同定.

    安川然太, 北島健, 佐藤ちひろ

    第81回日本生化学会大会  2008.12.9 

     More details

    Language:English   Presentation type:Poster presentation  

  142. 哺乳類シアロ糖タンパク質のデアミノノイラミン酸 (KDN) 修飾糖タンパク質への改変. International conference

    中島優子, 藤田明子, 佐藤ちひろ, 北島健

    第81回日本生化学会大会  2008.12.9 

     More details

    Language:Japanese   Presentation type:Poster presentation  

  143. 化学的検出法を用いた天然におけるサイクリックシアル酸の局在検索. International conference

    岩田章子, 佐藤ちひろ, 安藤弘宗, 石田秀治, 木曽真, 神奈木玲児, 北島健

    第81回日本生化学会大会  2008.12.9 

     More details

    Language:Japanese   Presentation type:Poster presentation  

  144. ポリシアル酸の新機能 International conference

    佐藤ちひろ

    第6回日本糖鎖科学コンソーシアムシンポジウム-糖鎖研究と他領域との統合-  2008.12.3 

     More details

    Language:Japanese   Presentation type:Symposium, workshop panel (nominated)  

  145. Novel insights into the novel binding sites of the sialic acid biding Ig-like lectin-7 (Siglec-7) gained by in silico docking studies.

    A one-day symposium on Sialyltransferases, Functional Glycomics, and Genomics of Zebrafish,  2008.11.18 

     More details

    Language:English   Presentation type:Symposium, workshop panel (nominated)  

  146. Complex formation between polysialic acid and brain derived neurotrophic factor (BDNF).

    Chihiro Sato, Yukihiro Kanato, Ken Kitajima

    24th International Carbohydrate Symposium,  2008.7.27 

     More details

    Language:English   Presentation type:Poster presentation  

  147. In vitro and in silico docking studies of heat shock protein (Hsp) 70 with sulfatide.

    Nao Yamakawa, Yoichiro Harada, Chihiro Sato, Gerard Vergoten, Ken Kitajima

    24th International Carbohydrate Symposium,  2008.7.27 

     More details

    Language:English   Presentation type:Poster presentation  

  148. Brain-derived neurotrophic factor binds to polysialic acid directly depending on degree of polymerization.

    Yukihiro Kanato, Ken Kitajima, Chihiro Sato

    SialoGlyco Conference 2008  2008.7 

     More details

    Language:English   Presentation type:Oral presentation (invited, special)  

  149. Occurrence of the Cyclic Sialic Acid in Mammalian Cells and Tissues As Demonstrated by Chemical Methods.

    K. Kitajima, S.Iwata, N. Kawakami, C. Sato, H. Ando, H. Ohno, H. Ishida, M. Kiso, O. Taguchi, R. Kannagi

    SialoGlyco Conference 2008  2008.7 

     More details

    Language:English   Presentation type:Oral presentation (invited, special)  

  150. In silico binding studies predict novel sialic acid binding sites on the sialic acid binding Ig-like lectin-7 (Siglec-7)

    Nao Yamakawa, Chihiro Sato, Paul R. Crocker, Gérard Vergoten, Ken Kitajima: I

    SialoGlyco Conference 2008  2008.7 

     More details

    Language:English   Presentation type:Poster presentation  

  151. Identification of the nuclear export signals that regulate the intracellular localization of the mouse CMP-sialic acid synthetase.

    Akiko Fujita, Chihiro Sato, Ken Kitajima

    SialoGlyco Conference 2008  2008.7 

     More details

    Language:English   Presentation type:Oral presentation (invited, special)  

  152. One-pot Enzymatic Synthesis of CMP-[14C]deaminoneuraminic Acid (KDN).

    Yuko Nakajima, Akiko Fujita, Chihiro Sato, Ken Kitajima

    SialoGlyco Conference 2008  2008.7 

     More details

    Language:English   Presentation type:Poster presentation  

  153. Chain Length Diversity of Sialic Acids and Its Biological Significance International conference

    SATO Chihiro

    Trends in Glycoscience and Glycotechnology  2004.9  FCCA(Forum: Carbohydrates Coming of Age)

     More details

    Language:English   Presentation type:Oral presentation (general)  

    Sialic acids are commonly present as monosialyl residues at the non-reducing terminal end of glycoconjugates. In some cases, &alpha;2&rarr;8 linked di/oligosialic acid chains with DP 2 or 3 sialic acid residues are common structural units of gangliosides, and involved in various biological processes, such as cell adhesion, cell differentiation, signal transduction, and surface expression of stage specific antigen. In contrast, little attention has been paid to the occurrence and functions of such short sialyl di/oligomers on glycoproteins, while polysialic acid (DP&ge;8) in embryonic NCAM has been extensively studied as a regulator of cell adhesion in neurogenesis. As analytical methods to detect di/oligosialic acid structures have been improved, several glycoproteins containing di/oligo/polysialic acid chains have been identified. It is thus hypothesized that these di/oligosialic acid residues on glycoproteins may have similar important functions in common with those proposed for the gangliosides or may have new functions. Currently, several studies showing the importance of di/oligosialic acid-containing glycoproteins have emerged. In this review, recent advances in such studies of di/oligosialic acid residues on glycoproteins, including analytical methods, occurrence, biosynthetic pathways, and functions, are described.

  154. Preface for the Special Issue Entitled &ldquo;New Waves in Sialobioscience&rdquo; International conference

    Sato Chihiro

    Trends in Glycoscience and Glycotechnology  2004.9  FCCA(Forum: Carbohydrates Coming of Age)

     More details

    Language:English   Presentation type:Oral presentation (general)  

  155. Sialic acid-dependent raft-raft interactions at fertilization

    XVIth International Symposium on glycoconjugates  2001.8.19 

     More details

    Language:English   Presentation type:Oral presentation (invited, special)  

  156. Identification of a new diSia-containing glycoprotein (adipoQ) in bovine and mouse sera

    XVIth International Symposium on Glycoconjugates  2001.7.19 

     More details

    Language:English   Presentation type:Poster presentation  

    Congress Center The Hague Netherlands

  157. 哺乳動物に見いだされるオリゴ・ポリシアル酸含有糖タンパク質ファミリーの構造と機能 International conference

    佐藤ちひろ

    第43回FCCAセミナー  2001.7.14 

     More details

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    "グランシップ, 静岡"

  158. "Identification of adipo Q, an adipocyte-specific glycoprotein, as a new oligosialic acid-containing glycoprotein and a possible substrate for the a2,8-sialyltransferase III (ST8SiaIII)."

    "International Symposium on Protein Traffic, Glycosylation, and Human Health"  2001.5.12 

     More details

    Language:English   Presentation type:Poster presentation  

  159. 糖タンパク質糖鎖におけるジおよびオリゴシアル酸の構造と機能 International conference

    佐藤ちひろ

    糖鎖物質設計シンポジウム  2000.8.1 

     More details

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

  160. "Expression and glycosylation of a major, carbohydrate-rich, cortical alveolus glycoprotein (hyosophorin) during oogenesis of medaka fish, Oryzias latipes"

    3rd International Symposium of the Molecular and Cellular Biology of the Egg and Embryo  2000.8.1 

     More details

    Language:English   Presentation type:Poster presentation  

  161. Di- and oligosialic acids as novel carbohydrate epitopes in leukocytes

    7th Conference on human leukocyte differentiation antigens  2000.6.1 

     More details

    Language:English   Presentation type:Poster presentation  

  162. Membrane microdomains are involved in carbohydrate-dependent sperm-egg interaction in sea urchin

    3rd International Symposium of the Molecular and Cellular Biology of the Egg and Embryo  2000.1.1 

     More details

    Language:English   Presentation type:Oral presentation (invited, special)  

  163. Lipid raft on gametic cells as a functional domain for sperm-egg interaction coupled with signal transduction

    The International Symposium on Development and Fertilization of Sea Urchin and Marine Invertebrates  1999.12.1 

     More details

    Language:English   Presentation type:Oral presentation (invited, special)  

  164. Egg receptor for sperm binds to gangliosides in the low density detergent-insoluble membrane (LD-DIM) domain of the sperm surface. Possible involvement of the LD-DIM in sperm-egg binding coupled with signal transduction during fertilization.

    XVth International Symposium on Glycoconjugates  1999.8.1 

     More details

    Language:English   Presentation type:Poster presentation  

  165. Glycosphingolipid enriched microdomain on gametic cells as functional domain for sperm-egg interaction coupled with signal transduction

    Gordon Research Conference on Fertilization and Activation of the Development  1999.6.1 

     More details

    Language:English   Presentation type:Oral presentation (invited, special)  

  166. オリゴシアル酸含有糖タンパク質の新しい化学的および免疫化学的解析法の開発と哺乳動物細胞における普遍的存在の証明 International conference

    佐藤ちひろ, 本田直輝, 太田薫, 福岡秀幸, 井上貞子, 松田幹, 北島健

    第71回日本生化学会大会  1998.10.16 

     More details

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

  167. "Expression of a2,8-linked oligosialic acid-containing glycoproteins in mammalian cells, depending on development, cell differentiation, and overexpression of polysialyltransferase genes."

    XIXth International Carbohydrate Symposium  1998.8.1 

     More details

    Language:English   Presentation type:Oral presentation (invited, special)  

  168. Frequent occurrence of oligosialyl units in vertebrate glycoproteins as evidenced by new chemical and immunochemical methods.

    XIVth International Symposium on Glycoconjugates  1997.9.27 

     More details

    Language:English   Presentation type:Oral presentation (invited, special)  

▼display all

Works 8

  1. Specific labeling and chemical analysis of sialic acid and sialyloligo/polymers

    2011

  2. Enzyme assay of polysialyltransferase (

    2011

  3. Carbohydrate analysis by gas-liquid chromatography

    2011

  4. Enzyme assay of polysialyltransferase (

    2011

  5. Sialic acid and evolution

    2006

  6. Function of polysialic acid in nervus system

    2004

  7. Essentials of glycobiology

    2003

  8. Biosynthesis of oligo/polysialic acid

    2002

▼display all

Research Project for Joint Research, Competitive Funding, etc. 4

  1. ポリシアル酸と相互作用する分子の解明

    2010.4 - 2011.3

    公益信託 林女性自然科学研究者研究助成基金 

      More details

    Grant type:Competitive

  2. 脳機能に関わるシアル酸重合体と相互作用する分子の同定とその応用基盤の確立

    2007.4

    加藤記念研究助成 

      More details

    Grant type:Competitive

  3. ミクログリア細胞におけるポリシアル酸の存在とその機能解析

    2006

  4. 神経形成を制御する新規糖鎖オリゴシアル酸の作用機構の解明

    2003.4 - 2004.3

    水谷科学振興財団2003年度研究助成 

      More details

    Grant type:Competitive

KAKENHI (Grants-in-Aid for Scientific Research) 21

  1. Molecular mechanism of new cellular functions mediated by polysialic acid

    Grant number:15K06995  2015.4 - 2018.3

    SATO Chihiro

      More details

    Authorship:Principal investigator 

    Grant amount:\4940000 ( Direct Cost: \3800000 、 Indirect Cost:\1140000 )

    Polysiali acid (polySia/PSA) is present in brain and is involved in learning, memory and social interaction. So far, we have been demonstrated that polySia is involved in brain functions not only due to its large exclusion volume but also due to molecule binding properties. In this research, we demonstrated that polySia chains show characteristic features towards molecule-binding properties via biosynthetic enzymes, ST8SIA2 and ST8SIA4.

  2. Synthesis of chemical probes containing di/oligosialialic acids and their biological evaluation related to innate immunity

    Grant number:26282209  2014.4 - 2017.3

    Tanaka Hiroshi

      More details

    Authorship:Coinvestigator(s) 

    In this report, we first investigated that partially unprotected sialyl donors underwent a-selective glycosidation without use of an acetonitrile effect. To demonstrate the utility of the method, a straight forward synthesis of α(2,9) disialosides containing N-acetyl and/or N-glycolyl groups was achieved by using the two N-acetyl and N-glycolyl sialyl donors. Next we successfully prepared Glycopolymers possessing disialic acids with a narrow with narrow polydisparities from glycosylated monomers via π-allyl nickel catalyzed coordinating polymerization. A biological evaluation of the glycopolymers and revealed that the synthetic fluoroecent-labelled glycopolymers cause the Siglec-7-GD3 interaction to dissociate in the micromolar concentration range. Finally we prepared the synthesis of dextran derivative possessing disialic acids via polymer reactions. The glycopolymer was able to release Siglec7 from Siglec7-GD3 complex possibly.

  3. 精神疾患に関与するポリシアル酸転移酵素の機能解析

    Grant number:26110708  2014.4 - 2016.3

    新学術領域研究(研究領域提案型)

    佐藤 ちひろ

      More details

    Authorship:Principal investigator 

    Grant amount:\9360000 ( Direct Cost: \7200000 、 Indirect Cost:\2160000 )

    本研究は統合失調症などの精神疾患に関連するポリシアル酸転移酵素ST8SIA2の機能解析が目的であり、本研究では報告のある精神疾患患者に有意なSNPsや変異に関して、それらの酵素がもたらす産物であるポリシアル酸の構造および機能解析を行った。具体的にはプロモーターに存在するrSNPに関してはプロモータ解析および遺伝子のマイクロアレイの解析を各種細胞を用いて行い、ある転写因子によって細胞特異的にST8SIA2の発現異常が起こる可能性を示した。翻訳領域に存在しアミノ酸置換を伴うSNPの解析から、疾患型が合成するポリシアル酸が損なわれ、特にBDNFの結合やproBDNFの結合性が正常型より損なわれることがわかった。また翻訳領域に存在しアミノ酸置換を伴わないsSNPは、コドン使用頻度によりST8SIA2の翻訳速度が変化し、ポリシアル酸の生合成に影響を与える可能性が示唆された。イントロンに存在するiSNPは、特に双極性障害型のSNPに関して、マウスの神経細胞およびヒトHEK細胞においてST8SIA2の合成が促進され、polySiaが過剰発現することが明らかになった。ST8SIA2の過剰発現の影響を調べるために子宮内エレクトロポレーション法により、ST8SIA2およびST8SIA4の遺伝子導入を行ったところ、ニューロンの移動がMockに比べて変化した。以上のことより、ST8SIA2遺伝子発現は細胞特異的に発現が厳密に制御されており、それに伴いpolySiaの構造が損なわれることが分かった。つまり、polySia構造は厳密に構造が制御されており、それによって神経作用因子であるBDNF、FGF2, proBDNFの結合性を制御することなどで、正常な神経機能を果たすことが考えられた。
    27年度が最終年度であるため、記入しない。
    27年度が最終年度であるため、記入しない。

  4. Novel regulatory mechanism for intracellular calcium ion concentration by membrane microdomain-localized glycans in sperm

    Grant number:25292216  2013.4 - 2016.3

    Kitajima Ken

      More details

    Authorship:Coinvestigator(s) 

    Many studies using glycan-related probes have reported the importance of glycan chains in fertilization. However, glycan-related gene-modified studies have told that glycans are not always essential for a successful fertilization. It is thus a currently important subject to re-evaluate molecular mechanisms for glycan-involved events at fertilization. To solve the subject, we focused on the "membrane microdomain" that is molecular complex of proteins and lipids with highly concentrated glycans on plasma membrane. In this study, we demonstrated the followings: (1) Glycan-enriched GPI-anchor peptide on the sperm membrane microdomains ubiquitously occurs not only in sea urchin and pig, but also avians and amphibians that have never been shown to contain such GPI-anchored molecules before; (2) These glycans are involved in sperm motility through regulating the intracellular calcium ion concentration in sperm.

  5. 微小糖鎖空間における酸性多糖が織りなす神経制御機構の解明

    2012.4 - 2014.3

    科学研究費補助金  新学術領域

    佐藤ちひろ

      More details

    Authorship:Principal investigator 

  6. 細胞表面酸性多糖が織りなす微小糖鎖空間による神経機能調節機構の解明

    Grant number:24110506  2012.4 - 2014.3

    新学術領域研究(研究領域提案型)

    佐藤 ちひろ

      More details

    Authorship:Principal investigator 

    Grant amount:\7150000 ( Direct Cost: \5500000 、 Indirect Cost:\1650000 )

    ポリシアル酸はシアル酸が8-400残基縮重合した構造の総称で、胎児脳の神経細胞接着分子(NCAM)を特異的に修飾する。成体脳ではその大部分が消失するが、海馬や嗅球といった神経新生の盛んな領域に発現が継続している。ポリシアル酸の機能として反接着作用が知られていたが、我々はポリシアル酸が自身に神経機能を制御する分子、BDNF、FGF2,ドーパミンなどを結合し、その機能を制御しうることを明らかにしてきている。本研究では、(1) polySiaによる神経作用因子の保持・放出機構の解明、(2) poySiaの"質と量"を制御する生合成機構の解明、(3) polySiaと統合失調症などの精神疾患との関わりの解明をすすめること目指した。(1)に関してはpolySiaが、自身の質と量を調節することにより、神経作用因子を保持状態を制御することがわかった。またその受容体への受け渡し機構には、より高親和性受容体への直接受け渡し、共受容体への間接的な受け渡し、polySia鎖の構造変化による受け渡しなど、さまざまな機構が働いていることが初めて明らかになった。(2)では、polySiaの質と量を制御する機構としてポリシアル酸転移酵素(ST8SIA2)に着目し、その変異が生合成する機構を一部明らかにした。(3)においては、統合失調症患者で明らかになったST8SIA2のSNP-7およびSNP-9の解析を正常型とあわせて行うことにより、その生合成酵素の産物の不全がpolySia鎖の不全を引き起こし、ひいては分子結合性における不全がおきることによるシグナル伝達の不具合がおこることを示した。以上の結果より、本研究において、polySia鎖がつくり出す細胞膜近傍における空間において、自身の分子結合性により細胞機能を制御していること、このpolySia鎖の生合成は高度の制御されていることが強く示唆された。
    25年度が最終年度であるため、記入しない。
    25年度が最終年度であるため、記入しない。

  7. Regulation of neural functions via polySia and FGF2 interaction

    Grant number:23570133  2011 - 2013

    SATO Chihiro

      More details

    Authorship:Principal investigator 

    Grant amount:\5460000 ( Direct Cost: \4200000 、 Indirect Cost:\1260000 )

    Polysialic acid (polySia) is a homopolymer of sialic acid with the degree of polymerization 8-400 Sia residues. PolySia is highly expressed on the neural cell adhesion molecule (NACM) in embryonic brains. In adult, polySia mostly disappeared; however, it persists in distinct regions such as hippocampus, subventricular zone, thalamus, prefrontal cortex and amygdala, where neural plasticity, remodeling of neural connections or neural generation are ongoing. PolySia on NCAM is known to have anti-adhesive effects on cell-cell interactions due to its bulky polyanionic nature and is involved in regulation of neurogenesis and neuronal functions. In this study, we demonstrate that polySia functions as a reservoir scaffold for various molecules of neurological active molecules, such as fibroblast growth factor 2 (FGF2).

  8. Discovery of the insect-type sialic acid and its metabolic pathway

    Grant number:23658287  2011 - 2012

    KITAJIMA Ken

      More details

    Authorship:Coinvestigator(s) 

    The objective of this study is to demonstrate the hypothesis that insects do not contain usual sialic acids (Sia) that are commonly found in vertebrates, but “insect-type Sia”, which is unique to insects. Using Drosophilla melanogaster (fluit fly), Tribolium castaneum (red flour beetle), several moth species, purification and analyses of structure, occurrence, and metabolic pathways of the insect -type Sia were performed. We could identify the insect-type Sia that was present in all the insects tested in this study. For metabolic aspects, all insects do not contain metabolic enzymes known for the common Sia, and even in such insects that were shown to contain the metabolic enzymes, the enzymes seemed to lose their activities. It would be an urgent subject, however, to see if the insect enzymes can utilize the insect-type Sia in near future. Taken together, our hypothesis that insects contain the insect-type Sia, but not the common Sia has been successfully verified.

  9. Functional study on large glycans on the surface of developing embryos

    Grant number:22380187  2010 - 2012

    KITAJIMA Ken

      More details

    Authorship:Coinvestigator(s) 

    To address a question why the extremely large glycans are attached on the embryonic cell surface, we studied on two examples of glycoprotein that had been found in madaka fish by ourselves. First, the protein with LeX-containing large glycans, named LeX-gp, was successfully purified. The glycans were shown to contain a multiply tandem repeat of LeX-structure, and to be involed in cell adhesion. Second, the egg cortical vesicular glycoprotein that is secreted from egg at fertilization, named hyosophorin, was found to be involved in cortical reaction or egg activation and the progress of early development of medaka. We revealed that the peptide part is important for its cell proliferation stimulating activity through its binding to a specific receptor, while the glycan part is rather required for the cortical reaction than for the cell growth of embryonic cells. Taken together, we postulate a hypothesis that , once associated with the cell surface, the huge glycans drastically change the surface atmosphere to affect the physiology of cell significantly.

  10. Interaction between BDNF and polysialic acid localized in brains

    Grant number:20570107  2008 - 2010

    SATO Chihiro

      More details

    Authorship:Principal investigator 

    Grant amount:\4810000 ( Direct Cost: \3700000 、 Indirect Cost:\1110000 )

    Polysialic acid (polySia) is the anionic polysaccharide expressed mainly in vertebrate brains. PolySia modifies embryonic NCAM and functions as a negative regulator between cell-cell/extracellular matrix via its bulky and exclusive features. In this study, we demonstrated that polySia directly binds to a brain derived neurotrophic factor (BDNF) and that through its interaction it regulates the ligand-receptor interactions.

  11. 脳に局在するポリシアル酸構造と脳由来神経栄養因子との相互作用の解析       

    2008

    科学研究費補助金  基盤研究(C),課題番号:20570107

    佐藤 ちひろ

      More details

    Authorship:Principal investigator 

  12. Regulatory mechanisms of fertilizability of animal sperm by the surface glycans

    Grant number:19380192  2007 - 2009

    KITAJIMA Ken

      More details

    Authorship:Coinvestigator(s) 

    To understand roles of the surface glycans in regulation of fertilizability of animal sperm, we focused on the glycan-rich glycoproteins whose glycan content was more than 90% by weight. The following two new findings were obtained : (1) Glycan-rich flagellar glycoproteins were discovered in pig and sea urchin, and found to regulate sperm motility through controlling the intracellular calcium ions ; (2) Another type of glycan-rich glycoproteins strongly sticking on the sperm surface was found to be originated from seminal plasma. The detachment of the glycoprotein from the surface during capacitation was related with the higher fertilizability of sperm.

  13. ミクログリア細胞におけるポリシアル酸の存在とその機能解析

    2006.4 - 2008.3

    科学研究費補助金  若手研究(B)

      More details

    Authorship:Principal investigator 

  14. ミクログリア細胞におけるポリシアル酸の存在とその機能解析

    Grant number:18770083  2006 - 2007

    佐藤 ちひろ

      More details

    Authorship:Principal investigator 

    Grant amount:\3700000 ( Direct Cost: \3700000 )

    ポリシアル酸はシアル酸重合体の総称であり、魚卵糖タンパク質(PSGP)や神経細胞接着分子(NCAM)にその存在が知られている。ポリシアル酸の機能は、NCAMを中心に解析が進んでおり、かさ高い排除体積とポリアニオニックな性質による反接着作用を持つことが知られている。我々はこのミクログリア細胞に注目してポリシアル酸の存在検索および発現解析を行い、ミクログリア細胞株Ra2におけるポリシアル酸の存在をポリシアル酸に特異的な抗体12E3および特異的な酵素Endo-Nを用いて証明した。またRa2細胞に対してLPS刺激を行うとポリシアル酸が完全に消失することを見出した。またRa2細胞のLPS刺激によるポリシアル酸構造消失の分子機構を明らかにするために、分解過程に注目して実験を行った。シアル酸の分解に関わる酵素としてシアリダーゼに着目し、まず、シアリダーゼ遺伝子(Neu)の発現解析を行った。その結果、Neu2とNeu4が活性化により変動していることが分かった。さらに、実際にシアリダーゼ活性がポリシアル酸の消失に関わっていることを明らかにするために、シアリダーゼ阻害剤存在下でLPS刺激を行い、細胞表面のポリシアル酸の存在を調べた。同時に培地におけるシアリダーゼ活性を測定した。その結果、ポリシアル酸の消失にシアリダーゼが関わっていることが初めて明らかになった。一方、Ra2に対し、神経保護的な作用をもつサイトカインで刺激を行うと、ポリシアル酸構造が逆に増加する傾向があることも明らかにした。これまで、ポリシアル酸の素早い変動に関わる知見は得られておらす、ポリシアル酸構造の制御メカニズムの解明が期待される。

  15. Identification and fimoticual analyses ofglycoproteins responsible for sperm-egg interaction in birds

    Grant number:17380200  2005 - 2007

    MATSUDA Tsukasa

      More details

    Authorship:Coinvestigator(s) 

    From a viewpoint of cell-cell interaction, Structural and functional analyses have been done on two kinds of glycoproteins (ZP glycoprotein of zona pellucid and phospholipid-binding protein, MFG-ES, of sperm surface),which have been suggested to be involved in specific recognition and binding for gamete interaction. As for the ZP glycoprotein, ligand blotting and immuniprecipitation analyses demonstrated that ZPB1 specifically bound to ZPC and thereby disulfide-bridged ZPB1 dimmerization was induced. Such ZPC-induced ZPB1 conformational change was suggested to be responsible for acquisition of sperm activation ability. Furthermore, ZPB1 interacted with ZPC secreted from transfected COS cells and formed insoluble matrixes with ZPC on the cell surface. In vitro incubation of ZPB1 with ZPC spontaneously produced fibrous aggregates of ZPB1-ZPC hetero complexes, which were visible under optical microscopy and morphologically resembled the aggregates obtained from mechanically decomposed chicken egg-envelope. Formation of such fibrous aggregates depended on ZPC/ZPB1 ratio, and involved ZPB1 dimerization through disulfide cross-linking, which had been found in authentic egg-envelope developed in hen's ovary. Furthermore, addition of excessive amounts of ZPC to ZPB1 produced soluble but high molecular weight heterocomplexes with increased adherence property against polystyrene ELISA plates. Thus, the specific association between ZPB1 and ZPC could play pivotal roles to initiate complex formation of hetero-polymers of ZP proteins in egg-envelope matrix construction. As for MFG-E8, this phospholipid-binding protein was shown to be expressed in various types of cells other than sperm by mRNA analyses of various animal cells and tissues, and some important roles in the differentiation and function of adipocytes and the apoptosis of mammary epithelial cells were suggested as a membrane protein on outer surface of microvesicles, termed exosome. Presence of MFG-ES on exosomes suggested contribution of membrane microvesicles to sperm-egg interaction and importance of complex supermolecular interaction among ZP-glycoproteins and the membrane-vesicle constituent molecules, including MFG-E8.

  16. 糖タンパク質のユニークなシアル酸重合体修飾の機能解析

    Grant number:17046006  2005 - 2006

    佐藤 ちひろ

      More details

    Authorship:Principal investigator 

    Grant amount:\4500000 ( Direct Cost: \4500000 )

    我々は、これまでの糖タンパク質上のシアル酸の存在状態に対する認識(モノ【tautomer】ポリ)を塗り替え、新たな概念(モノ【tautomer】オリゴ【tautomer】ポリ)を創出した。すなわちオリゴシアル酸の存在を仮定し、シアル酸重合体の微量検出方法を新たに開発し、その方法を用いてその存在証明を行った。本研究は糖タンパク質上のシアル酸重合体というユニークな糖鎖修飾が、どのような生物学的意義を持つのかを明らかにすることが目的である。本研究ではこれまで明らかになっていないシアル酸重合体の生物学的な意義の解明をT細胞、体液、ミクログリア細胞、生殖細胞を用いて試みた。T細胞では、糖タンパク質と糖脂質の共通糖鎖エピトープ構造である、ジシアル酸構造がラフトに存在し、T細胞の活性化に関わっていること、その際にST8Sia I, IV, VIの変動が起こっていることを明らかにした。また、体液においては、マウス血清中のplasminogen, vitronectin, IgLがジシアル酸構造修飾を受けていることを明らかにし、特にvitronectinのジシアル酸構造は肝切除後に減少するということが示した。また、脳においてはマウスミクログリア細胞にポリシアル酸構造が存在することを証明した。特にLPSで炎症性の刺激を行うと、ポリシアル酸構造は消失するが、IL4などの神経保護的な作用をもつサイトカインで刺激を行うと、ポリシアル酸構造が増加する傾向があることを明らかにした。特にLPS刺激によるポリシアル酸の消失はポリシアル酸合成に関わるST8SiaのmRNAの発現の減少だけでなく、シアリダーゼのmRNAの増加も同時におこっており、実際にシアリダーゼの活性の上昇が見られた。生殖細胞はウニ精子を用いた。このポリシアル酸構造は通常見られるα2,8結合ポリシアル酸構造ではなく、α2,9ポリシアル酸構造であった。この構造を持つflagellasialinを新規にクローニングし、同定を行った。製麻区表面上のα2,9ポリシアル酸構造はCaチャンネルを介した精子の鞭毛の運動性に関わっていることを明らかにした。

  17. 糖タンパク質のユニークなシアル酸重合体修飾の機能解析

    2005

    科学研究費補助金  特定領域研究,課題番号:17046006

    佐藤 ちひろ

      More details

    Authorship:Principal investigator 

  18. 神経細胞におけるシアル酸重合体の多様性と生物機能の解析

    Grant number:16770073  2004 - 2005

    佐藤 ちひろ

      More details

    Authorship:Principal investigator 

    Grant amount:\3300000 ( Direct Cost: \3300000 )

    シアル酸重合体とは、シアル酸同士が2〜数十残基結合した構造である。胎仔脳において、神経細胞やグリア細胞におけるNCAM上のポリシアル酸構造の研究はよく行われており、反接着分子として機能することにより、脳の発達に深く関わることが知られている。一方、脳内での免疫機構を司るマクロファージ様の細胞であるミクログリア上のシアル酸構造に関してこれまで解析が全くなされていなかった。我々はマウス新生児脳のプライマリーカルチャーおよびノミクログリア細胞株を用いて、ミクログリア細胞にジシアル酸構造だけでなくポリシアル酸が存在することを明らかにした。また、ミクログリア細胞はその活性化によって、神経組織に対し、trophicに働いたり、toxicに働くことが知られているが、その機構について詳しくはわかっていない。そこでミクログリアの機能におけるポリシアル酸構造の関わりを明らかにするために、まず、ミクログリア細胞株を用いて、活性化状態におけるポリシアル酸の変動を明らかにすることとした。LPSおよびIL4刺激という二つの異なる活性化状態におけるポリシアル酸構造の変動を、免疫染色および我々の開発したオリゴ・ポリシアル酸の化学的検出法により解析した。その結果、ミクログリア細胞のポリシアル酸はLPS刺激により速やかに消失すること、またIL-4刺激においてはそのポリシアル酸量が増加することが明らかになった。これまで、ポリシアル酸の消失は、脳の発達に伴う数週間から数ヶ月にわたる変動と考えられていたが、ミクログリアにおけるポリシアル酸の速やかな消失は、分単位という非常に素早い変化であった。このような素早い変動はポリシアル酸の研究のおいて初めての例である。また、この急激な消失はポリシアル酸がミクログリアの活性化に関わりがあることを示唆する。

  19. 神経細胞におけるシアル酸重合体の多様性と生物機能の解析

    2004

    科学研究費補助金  若手研究(B),課題番号:16770073

    佐藤 ちひろ

      More details

    Authorship:Principal investigator 

  20. Intra-intestinal kinetics and absorption mechanism of digestion-resisitant maclomolecular food components

    Grant number:14360073  2002 - 2004

    MATSUDA Tsukasa

      More details

    Authorship:Coinvestigator(s) 

    Egg albumin (ovalbumin:OVA) is resistant to proteolytic hydrolysis and known to be a major allergenic protein in egg allergy. By using egg albumin as a model macromoleculer food component, the absorption of OVA from mouse intestinal tract into body and the kinetics of OVA in circulating peripheral blood were investigated. After oral administration of OVA (10 mg) to mice, peripheral blood was collected and then serum was separated from coagulated blood. The OVA concentration in the serum was determined to be 10-50 ng/ml by EUSA, and the OVA in the serum was concentrated by immuno-affinity precipitation using anti-OVA antibodies coupled to Sepharose. In SDS-PAGE analysis, the OVA recovered from peripheral blood revealed a single major protein band with a molecular mass identical to intact OVA and a minor band with a slightly smaller molecular mass. No band corresponding to OVA fragments with smaller molecular masses, which had been demonstrated to be the major components of OVA antigens in the mouse small intestine. Furthermore, two-dimensional gel electrophoresis demonstrated that the OVA recovered from blood was composed of 4-6 protein spots with different pI but with the same molecular mass, and that the basic protein spots were dominant as compared with those of intacl OVA, suggesting that the pI of OVA was shifted to more basic ones during the intestinal absorption or the migration in circulating blood. Such a pI shift was not observed for OVA incubated in vitro with mouse serum. Taken together, it is suggested that a small portion of orally administered OVA could escape from intestinal proteolytic digestion and be absorbed as an intact form, but with a slight modification, across intestinal epithelium into the blood stream. Thus, it might be possible that orally administered bio-active proteins exert its biological function in peripheral tissues and organs.

  21. 神経形成を制御する新規糖鎖オリゴシアル酸の作用機構の解明

    Grant number:14780471  2002 - 2003

    佐藤 ちひろ

      More details

    Authorship:Principal investigator 

    Grant amount:\2100000 ( Direct Cost: \2100000 )

    シアル酸は糖タンパク質や糖脂質を構成する酸性糖で、様々な生物学的現象において非常に重要な役割を担っている。通常、1残基が糖鎖の最外部に結合したモノシアル酸として存在するが、まれにその末端シアル酸残基の上にさらにシアル酸の直鎖ポリマーが結合するポリシアル酸構造として存在する。ポリシアル酸はその物理化学的性質により、細胞と細胞、細胞と細胞外基質の接着を阻害する方向に調節し、神経形成のモジュレーターとなっていることが明らかになってきた。一方、シアル酸の重合度が2から7のオリゴシアル酸構造の存在は糖タンパク質において、その存在はほとんど知られていなかった。我々は近年、オリゴシアル酸構造を微量で検出できる化学的方法、及び免疫化学的手法を世界で初めて開発し、それらの方法を用いて、哺乳動物の種々の臓器や組織において、オリゴシアル酸構造を持つ糖タンパク質が存在していること示した。本研究では、CD166がジシアル酸含有糖タンパク質であることを端緒に、T細胞上でも、神経細胞と同様にCD166がジシアロ糖タンパク質であり、活性化に関わることを示唆する知見を得た。またNeuro2A細胞で、ST8SiaVIが存在していることが明らかになった。さらにヒトミルク中にポリシアリル化をうけたCD36分子が存在し、発達段階特異的な発現変動をしていることを明らかにすると同時に、CD36はジシアリル化を受けていること、乳腺上皮細胞上に、ポリシアル酸とオリゴシアル酸を生合成する酵素の発現が、泌乳期特異的に変動することを明らかにした。さらに、HSP110ホモログであるウニ精子結合蛋白質がシアル酸結合レクチンであることが明らかになった。

▼display all

 

Teaching Experience (On-campus) 3

  1. バイオテクノロジー

    2020

  2. 糖鎖生物学特論

    2020

  3. 化学実験

    2020

Teaching Experience (Off-campus) 1

  1. 分子細胞機能学輪講I

    Nagoya University)