Updated on 2024/03/23

写真a

 
MURASE, Jun
 
Organization
Graduate School of Bioagricultural Sciences Field Science Center Division Professor
Graduate School
Graduate School of Bioagricultural Sciences
Undergraduate School
School of Agricultural Sciences Department of Bioresource Sciences
Title
Professor
Contact information
メールアドレス

Degree 1

  1. 博士(農学) ( 1994.10   名古屋大学 ) 

Research Interests 5

  1. wetland

  2. protists

  3. microbial food web

  4. methane

  5. 水稲根圏微生物

Research Areas 3

  1. Others / Others  / Biogeochemistry

  2. Others / Others  / Plant Nutrient Science/Soil Studies

  3. Others / Others  / Microbial Ecology

Current Research Project and SDGs 5

  1. 水田生態系における微生物群集の生態

  2. 農耕地土壌の光合成微生物の生態

  3. Diversity and function of protists in wetland soils

  4. 環北極陸域生態系のメタン動態

  5. 土壌生態系機能の解明を目指した人工土壌の開発

Research History 10

  1. Mie University

    2020.4 - 2023.3

  2. Nagoya University

    2015.4

  3. 中部大学応用生物学部 非常勤講師

    2008.4 - 2012.3

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    Country:Japan

  4. 名古屋大学大学院生命農学研究科 講師

    2007.4

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    Country:Japan

  5. 名古屋女子大学家政学部 非常勤講師

    2005

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    Country:Japan

  6. 名城大学農学部非常勤講師

    2003

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    Country:Japan

  7. 名古屋女子大学家政学部 非常勤講師

    2003

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    Country:Japan

  8. 名古屋大学大学院生命農学研究科 助手

    2001.4 - 2007.3

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    Country:Japan

  9. School of Environmental Science, University of Shiga Prefecture (Assist. Prof.)

    1996.4 - 2001.3

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    Country:Japan

  10. 名古屋大学農学研究科 日本学術振興会特別研究員

    1992.4 - 1994.3

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    Country:Japan

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Education 2

  1. Nagoya University   Graduate School, Division of Agriculture

    1989.4 - 1994.10

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    Country: Japan

  2. Nagoya University   Faculty of Agriculture

    1985.4 - 1989.3

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    Country: Japan

Professional Memberships 5

  1. 日本土壌肥料学会

  2. 日本微生物生態学会   評議員

    2012.1 - 2013.12

  3. 日本土壌微生物学会

  4. 日本陸水学会

  5. International Society of Microbial Ecology

Committee Memberships 1

  1. FAO Intergovernmental Technical Panel on Soils   Expert  

    2018.6 - 2022.5   

Awards 4

  1. Best poster award

    2013.10   Taiwan Society of Microbial Ecology  

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    Award type:Award from international society, conference, symposium, etc.  Country:Taiwan, Province of China

  2. 日本土壌肥料学会優秀ポスター賞

    2010.9   日本土壌肥料学会  

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    Country:Japan

  3. 日本土壌微生物学会優秀ポスター賞

    2009.6   日本土壌微生物学会  

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    Country:Japan

  4. 日本陸水学会 吉村賞

    2001.10   日本陸水学会  

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    Country:Japan

 

Papers 104

  1. Conversion from natural coniferous forests to cedar plantations increase soil nitrogen cycling through changing microbial community structures

    Sawada Kozue, Inagaki Yoshiyuki, Sugihara Soh, Kunito Takashi, Murase Jun, Toyota Koki, Funakawa Shinya

    APPLIED SOIL ECOLOGY   Vol. 191   2023.11

  2. Bacteriovory of enteric bacteria by soil isolated amoeba depends on both temperature and salinity

    Das, S; Meyer, I; Alaguvel, V; Ghazaryan, L; Murase, J; Shnerb, N; Gillor, O

    GEODERMA   Vol. 439   2023.11

  3. Arctic Plant Responses to Summer Climates and Flooding Events: A Study of Carbon and Nitrogen-Related Larch Growth and Ecosystem Parameters in Northeastern Siberia

    Liang, MC; Sugimoto, A; Tei, S; Takano, S; Morozumi, T; Shingubara, R; Murase, J; Maximov, TC

    JOURNAL OF GEOPHYSICAL RESEARCH-BIOGEOSCIENCES   Vol. 128 ( 10 )   2023.10

  4. Type-specific quantification of particulate methane monooxygenase gene of methane-oxidizing bacteria at the oxic-anoxic interface of a surface paddy soil by digital PCR

    Shinjo Rina, Oe Fumika, Nakagawa Koki, Murase Jun, Asakawa Susumu, Watanabe Takeshi

    ENVIRONMENTAL MICROBIOLOGY REPORTS     2023.4

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  5. Protists: the hidden ecosystem players in a wetland rice field soil (Feb, 10.1007/s00374-023-01705-9, 2023)

    Murase Jun, Asiloglu Rasit

    BIOLOGY AND FERTILITY OF SOILS     2023.3

  6. Arctic Plant Responses to Summer Climates and Flooding Events: A Study of Carbon and Nitrogen-Related Larch Growth and Ecosystem Parameters in Northeastern Siberia Reviewed International coauthorship

    Liang, Maochang Sugimoto, Atsuko Tei, Shunsuke Takano, Shinya Morozumi, Tomoki Shingubara, Ryo Murase, Jun Maximov, Trofim C.

    Journal of Geophysical Research: Biogeosciences   Vol. 128   page: e2022JG007135   2023

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    Language:English  

    DOI: https://doi.org/10.1029/2022JG007135

  7. Estimating Yield-Related Traits Using UAV-Derived Multispectral Images to Improve Rice Grain Yield Prediction Reviewed

    Bascon Maria Victoria, Nakata Tomohiro, Shibata Satoshi, Takata Itsuki, Kobayashi Nanami, Kato Yusuke, Inoue Shun, Doi Kazuyuki, Murase Jun, Nishiuchi Shunsaku

    AGRICULTURE-BASEL   Vol. 12 ( 8 )   2022.8

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.3390/agriculture12081141

    Web of Science

  8. eDNA解析を利用した土壌微生物機能への太陽熱土壌消毒の影響評価法 Reviewed

    浅川晋、村瀬潤、篠原陽子

    日本土壌肥料学雑誌   Vol. 92 ( 6 ) page: 476 - 480   2021.12

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    Authorship:Corresponding author   Language:Japanese   Publishing type:Research paper (scientific journal)  

  9. 土壌に関する政府間技術パネル(ITPS)作業グループ会合報告 Invited

    村瀬 潤

    日本土壌肥料学会誌   Vol. 92 ( 6 ) page: 501 - 502   2021.12

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    Authorship:Lead author, Last author, Corresponding author   Language:Japanese   Publishing type:Research paper (scientific journal)  

  10. Soil texture and properties rather than irrigation water type shape the diversity and composition of soil microbial communities Reviewed International coauthorship

    Obayomi Olabiyi, Seyoum Mitiku Mihiret, Ghazaryan Lusine, Tebbe Christoph C., Murase Jun, Bernstein Nirit, Gillor Osnat

    APPLIED SOIL ECOLOGY   Vol. 161   2021.5

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.apsoil.2020.103834

    Web of Science

  11. Top-down effects of protists are greater than bottom-up effects of fertilisers on the formation of bacterial communities in a paddy field soil International coauthorship

    Asiloglu Rasit, Kenya Kobayashi, Samuel Solomon Oloruntoba, Sevilir Bahar, Murase Jun, Suzuki Kazuki, Harada Naoki

    Soil Biology and Biochemistry   Vol. 156   2021.5

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.soilbio.2021.108186

    Web of Science

  12. Effect of protists on rhizobacterial community composition and rice plant growth in a biochar amended soil Reviewed International coauthorship

    Asiloglu Rasit, Sevilir Bahar, Samuel Solomon Oloruntoba, Aycan Murat, Akca Muhittin Onur, Suzuki Kazuki, Murase Jun, Turgay Oguz Can, Harada Naoki

    BIOLOGY AND FERTILITY OF SOILS   Vol. 57 ( 2 ) page: 293 - 304   2021.2

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1007/s00374-020-01525-1

    Web of Science

  13. Biochar affects taxonomic and functional community composition of protists Reviewed International coauthorship

    Asiloglu Rasit, Samuel Solomon Oloruntoba, Sevilir Bahar, Akca Muhittin Onur, Bozkurt Pinar Acar, Suzuki Kazuki, Murase Jun, Turgay Oguz Can, Harada Naoki

    BIOLOGY AND FERTILITY OF SOILS   Vol. 57 ( 1 ) page: 15 - 29   2021.1

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1007/s00374-020-01502-8

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  14. Soil: the great connector of our lives now and beyond COVID-19 Reviewed International coauthorship

    Poch Rosa M., dos Anjos Lucia H. C., Attia Rafla, Balks Megan, Benavides-Mendoza Adalberto, Bolanos-Benavides Martha M., Calzolari Costanza, Chabala Lydia M., de Ruiter Peter C., Francke-Campana Samuel, Prechac Fernando Garcia, Graber Ellen R., Halavatau Siosiva, Hassan Kutaiba M., Hien Edmond, Jin Ke, Khan Mohammad, Konyushkova Maria, Lobb David A., Moshia Matshwene E., Murase Jun, Nziguheba Generose, Patra Ashok K., Pierzynski Gary, Eugenio Natalia Rodriguez, Rojas Ronald Vargas

    SOIL   Vol. 6 ( 2 ) page: 541 - 547   2020.11

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    Language:English  

    DOI: 10.5194/soil-6-541-2020

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  15. Susceptibility and resilience of the soil ciliate community to high temperatures Reviewed

    Oshima Takaaki, Shinohara Yoko, Asakawa Susumu, Murase Jun

    SOIL SCIENCE AND PLANT NUTRITION   Vol. 66 ( 6 ) page: 870 - 877   2020.11

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    Authorship:Last author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1080/00380768.2020.1819148

    Web of Science

  16. Involvement of microaerophilic iron-oxidizing bacteria in the iron-oxidizing process at the surface layer of flooded paddy field soil Reviewed

    Nakagawa Koki, Murase Jun, Asakawa Susumu, Watanabe Takeshi

    JOURNAL OF SOILS AND SEDIMENTS   Vol. 20 ( 11 ) page: 4034 - 4041   2020.11

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  17. Protist-enhanced survival of a plant growth promoting rhizobacteria, Azospirillum sp. B510, and the growth of rice ( Oryza sativa L.) plants

    Asiloglu Rasit, Shiroishi Keiko, Suzuki Kazuki, Turgay Oguz Can, Murase Jun, Harada Naoki

    APPLIED SOIL ECOLOGY   Vol. 154   page: 103599   2020.10

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  18. Methane oxidation potential of the arctic wetland soils of a taiga-tundra ecotone in northeastern Siberia Reviewed International coauthorship

    Murase Jun, Sugimoto Atsuko, Shingubara Ryo, Liang Maochang, Morozumi Tomoki, Takano Shinya, Maximov Trofim C.

    SOIL SCIENCE AND PLANT NUTRITION   Vol. 66 ( 4 ) page: 645 - 652   2020.7

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    Authorship:Lead author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1080/00380768.2020.1786343

    Web of Science

  19. 田んぼの中の原生生物たちの暮らし Invited Reviewed

    村瀬 潤

    土と微生物   Vol. 74 ( 1 ) page: 26 - 31   2020.4

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    Authorship:Lead author, Last author, Corresponding author   Language:Japanese  

  20. Invention of Artificial Rice Field Soil: A Tool to Study the Effect of Soil Components on the Activity and Community of Microorganisms Involved in Anaerobic Organic Matter Decomposition

    Maeda Yu, Mise Kazumori, Iwasaki Wataru, Watanabe Akira, Asakawa Susumu, Asiloglu Rasit, Murase Jun

    MICROBES AND ENVIRONMENTS   Vol. 35 ( 4 )   2020

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  21. Diversity of 2-MIB-Producing Cyanobacteria in Lake Ogawara: Microscopic and Molecular Ecological Approaches. Reviewed

    Shizuka, K., Ikenaga, M., Murase, J., Nakayama, N., Kakino, W., Taruya, H., & Maie, N.

    Aquatic Science   Vol. 68   page: 9 - 23   2020

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  22. 水田への魚類の遡上を促す遡上板の開発 Reviewed

    皆川明子、若宮慎二、竹下邦明、佐川志朗、河口洋一、村瀬潤、都築隆禎、深澤洋二、江崎保男

    応用生態工学   Vol. 23 ( 1 ) page: 79 - 84   2020

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  23. Usability of water surface reflectance for the determination of riverine dissolved methane during extreme flooding in northeastern Siberia

    Morozumi Tomoki, Shingubara Ryo, Murase Jun, Nagai Shin, Kobayashi Hideki, Takano Shinya, Tei Shunsuke, Fan Rong, Maximov Trofim C., Sugimoto Atsuko

    POLAR SCIENCE   Vol. 21   page: 186 - 194   2019.9

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  24. Multi-year effect of wetting on CH4 flux at taiga-tundra boundary in northeastern Siberia deduced from stable isotope ratios of CH4

    Shingubara Ryo, Sugimoto Atsuko, Murase Jun, Iwahana Go, Tei Shunsuke, Liang Maochang, Takano Shinya, Morozumi Tomoki, Maximov Trofim C.

    BIOGEOSCIENCES   Vol. 16 ( 3 ) page: 755-768   2019.2

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.5194/bg-16-755-2019

    Web of Science

  25. Microhabitat segregation of heterotrophic protists in the rice (Oryza sativa L.) rhizosphere

    Asiloglu Rasit, Murase Jun

    RHIZOSPHERE   Vol. 4   page: 82 - 88   2017.12

  26. Quest of Soil Protists in a New Era

    Murase Jun

    MICROBES AND ENVIRONMENTS   Vol. 32 ( 2 ) page: 99 - 102   2017.6

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1264/jsme2.ME3202rh

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  27. Response of the protistan community of a rice field soil to different oxygen tensions (vol 92, fiw104, 2016)

    Takenouchi Yuriko, Iwasaki Kazufumi, Murase Jun

    FEMS MICROBIOLOGY ECOLOGY   Vol. 93 ( 5 )   2017.5

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1093/femsec/fix026

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  28. Fluctuations in the abundance of ammonia oxidizers and the contents of inorganic nitrogen in solarized soil

    Yamagata Mina, Shinohara Yoko, Maesaka Masahiro, Murase Jun, Asakawa Susumu

    SOIL SCIENCE AND PLANT NUTRITION   Vol. 63 ( 5 ) page: 434-440   2017

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1080/00380768.2017.1368035

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  29. Response of the protistan community of a rice field soil to different oxygen tensions Reviewed

    Takenouchi, Y., Iwasaki, K., Murase, J.

    FEMS Microbiology Ecology     2016

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    DOI: doi: 10.1093/femsec/fiw104

  30. Active community structure of microeukaryotes in a rice (Oryza sativa L.) rhizosphere revealed by RNA-based PCR-DGGE Reviewed

    Asiloglu, R., Murase, J.

    Soil Science and Plant Nutrition   Vol. 62   page: 440-446   2016

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  31. Impact of long-term fertilizer treatment on the microeukaryotic community structure of a rice field soil. Reviewed

    Murase, J., Hida, A., Ogawa, K., Nonoyama, T., Yoshikawa, N., Imai, K.

    Soil Biology and Biochemistry   Vol. 80   page: 237-243   2015

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  32. Impact of soil solarization on the ciliate community structure of a greenhouse soil. 61, 927-933 (2015) Reviewed

    Yokoe, K., Maesaka, M., Murase, J., Asakawa, S

    Soil Sci. Plant Nutr.   Vol. 61   page: 641-652   2015

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  33. Impact of soil solarization on the ciliate community structure of a greenhouse soil. Reviewed

    Murase, J., Shinohara, Y., Yokoe, K., Matsuda, R., Asakawa, S., Hashimoto, T.

      Vol. 60 ( 4 ) page: 927-933   2015

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  34. Community structure of microeukaryotes in a rice rhizosphere revealed by DNA-based PCR-DGGE. Reviewed

    Asiloglu, R., Honjo, H., Saka, N., Asakawa, S., Murase, J.

    Soil Sci Plant Nutr   Vol. 61   page: 761-768   2015

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  35. Abundance and composition of ammonia oxidizers in response to degradation of root cap cells of rice in soil microcosms. Reviewed

    Li, Y., Watanabe, T., Murase, J., Asakawa, S., Kimura, M.

    Journal of soils and sediments   Vol. 14   page: 1587-1598   2014

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  36. Branched and isoprenoid glycerol dialkyl glycerol tetraethers in soils and lake/river sediments in Lake Biwa basin and implications for MBT/CBT proxies. Reviewed

    Ajioka, T., Yamamoto, M., Murase, J.

    Organic Geochemistry   Vol. 73   page: 70-82   2014

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  37. Low nitrogen fertilization adapts rice root microbiome to low nutrient environment by changing biogeochemical functions. Reviewed

    Ikeda, S., Sasaki, K., Okubo, T., Yamashita, A., Terasawa, K., Bao, Z., Liu, D., Watanabe, T., Murase, J., Asakawa, S., Eda, S., Mitsui, H., Sato, T., Minamisawa, K.

    Microbes and Environments   Vol. 29   page: 50-59   2014

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  38. Growth of hydrogenotrophic and acetoclastic methanogens on substrate from rice plant callus cells in anaerobic soil: an estimation to the role of slough-off root cap cells to their growth Reviewed

    Li Y, Watanabe T, Murase J, Asakawa S, Kimura M

      Vol. 59   page: 548-558   2013

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  39. Identification of the major capsid gene (g23) of T4-type bacteriophages that assimilate substrates from root cap cells under aerobic and anaerobic soil conditions using a DNA-SIP approach Reviewed

    Li Y, Watanabe T, Murase J, Asakawa S, Kimura M

      Vol. 63   page: 97-105   2013

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  40. 安定同位体プロービング(SIP)法で探る水田土壌の物質動態と微生物 Invited Reviewed

    村瀬 潤

     土と微生物 67, 39-46 (2013)   Vol. 67   page: 39-46   2013

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    Authorship:Lead author   Language:Japanese  

  41. Growth of hydrogenotrophic and acetoclastic methanogens on substrate from rice plant callus cells in anaerobic soil: an estimation to the role of slough-off root cap cells to their growth. Reviewed

    Li, Y., Watanabe, T., Murase, J., Asakawa, S., Kimura, M.

    Soil Sci. Plant Nutr.   Vol. 59   page: 548-558   2013

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  42. Growth of methanogens in an oxic soil microcosm: Elucidation by a DNA-SIP experiment using 13C-labeled dried rice callus. Reviewed

    Li, Y, Lee CG, Watanabe T, Murase J, Asakawa S, Kimura M

      Vol. 58   page: 37-44   2012

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  43. Phylogenetic diversity of microturbellarians in Japanese rice paddy fields, with special attention to the genus Stenostomum. Reviewed

    Yamazaki, M, Murase, J, Asakawa, S, Kimura, M

    Soil Science and Plant Nutrition   Vol. 58   page: 11-23   2012

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  44. Incorporation of plant residue-derived carbon into the microeukaryotic community in a rice field soil revealed by DNA stable-isotope probing. Reviewed

    Murase J, Shibata M, Lee CG, Watanabe T, Asakawa S, Kimura M

      Vol. 79   page: 371-379   2012

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  45. 北方湿地生態系からのメタン放出に及ぼす積雪の影響 Invited Reviewed

    村瀬 潤

    低温科学   Vol. 70   page: 131-136   2012

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  46. Identification of microbial communities that assimilate substrate from root cap cells in an aerobic soil using a DNA-SIP approach. Reviewed

    Li, Y, Lee CG, Watanabe T, Murase J, Asakawa S, Kimura M

    Soil Biology & Biochemistry   Vol. 43   page: 1928-1935   2011

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  47. Assimilation of glucose-derived carbon into methanogenic archaea in soil under unflooded condition. Reviewed

    Watanabe T, Wang G, Taki K, Lee CG, Murase J, Asakawa S, Kimura M

    Applied Soil Ecology   Vol. 48   page: 201-209   2011

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  48. A new thermophilic heterolobosean amoeba, Fumarolamoeba ceborucoi, gen. nov., sp. nov., isolated near a fumarole at a volcano in Mexico. Reviewed

    Johan F. De Jonckheere JF, Murase J, Opperdoes FR

      Vol. 50   page: 43-50   2011

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  49. Bacterial populations assimilating carbon from 13C-labeled plant residue in soil: analysis by a DNA-SIP approach. Reviewed

    Lee CG, Watanabe T, Sato Y, Murase J, Asakawa S, Kimura M

      Vol. 43   page: 814-822   2011

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  50. Strain-specific incorporation of methanotrophic biomass into eukaryotic grazers in a rice field soil revealed by PLFA-SIP. Reviewed

    FEMS Microbiology Ecology   Vol. 75   page: 284-290   2011

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  51. Unique viral capsid assembly protein gene (g20) of cyanophages in the floodwater of a Japanese paddy field. Reviewed

    Wang GH, Murase J, Asakawa S, Kimura M

    Biol. Fert. Soils   Vol. 46   page: 93-102   2010

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  52. Isolation of a new heterolobosean amoeba from a rice field soil: Vrihiamoeba italica gen. nov., sp. nov. Reviewed

    Murase J, Kawasaki M, De Jonckheere JF

    Eur. J. Protistol.   Vol. 46   page: 164-170   2010

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  53. Isolation of a new heterolobosean amoeba from a rice field soil: Vrihiamoeba italica gen. nov., sp. nov. Reviewed

    Murase J, Kawasaki M, De Jonckheere JF

    European Jouranal of Protistology   Vol. 46   page: 164-170   2010

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  54. Low diversity of T4-type bacteriophages in applied rice straw, plant residues and rice roots in Japanese rice soils: Estimation from major capsid gene (g23) composition. Reviewed

    Fujihara S, Murase J, Tun CC, Matsuyama T, Ikenaga M, Asakawa S, Kimura M

    Soil Science and Plant Nutrition   Vol. 56   page: 800-812   2010

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  55. Novel cyanophage photosynthetic gene psbA in the floodwater of a Japanese rice field. Reviewed

    Wang GH, Murase J, Asakawa S, Kimura M

    FEMS Microbiol. Ecol.   Vol. 70   page: 79-86   2009

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  56. Methanogenic archaeal communities developed in paddy fields in the Kojima Bay polder, estimated by denaturing gradient gel electrophoresis, real-time PCR and sequencing analyses. Reviewed

    Watanabe, T., Cahyani, V.R., Murase, J., Ishibashi, E., Kimura, M., Asakawa, S.

    Soil Sci. Plant Nutr.   Vol. 55   page: 73-79   2009

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  57. Phyologenetic positions of Mn-oxidizing bacteria and fungi inhabiting Mn nodules in rice field subsoils. Reviewed

    Cahyani, V.R., Murase, J., Ikeda, A., Taki, K., Asakawa, S., Kimura,M

    Biol. Fertil. Soils   Vol. 45   page: 337-346   2009

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  58. T4-type bacteriophage communities estimated from the major capsid genes (g23) in manganese nodules in Japanese paddy fields. Reviewed

    Cahyani VR, Murase J, Ishibashi E, Asakawa S, Kimura M

    Soil Sci. Plant Nutr.   Vol. 55   page: 468-477   2009

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  59. Changes in major capsid genes (g23) of T4-type bacteriophages with soil depth in two Japanese rice fields. Reviewed

    Wang GH, Murase J, Taki K, Ohashi Y, Yoshikawa N, Asakawa S, Kimura M

    Biol. Fert. Soils   Vol. 45   page: 521-529   2009

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  60. Eukaryotic communities associated with the decomposition of rice straw compost in a Japanese rice paddy field estimated by DGGE analysis Reviewed

    HatamotoM., Tanahashi T., Murase J., Matsuya M., Hayashi M., Kimura M., Asakawa S.

    Bio. Fert. Soils   Vol. 44 ( 3 ) page: 527-532   2008

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    To estimate the succession and phylogenetic composition of the eukaryotic communities responsible for the decomposition of rice straw compost under flooded conditions during the cultivation period of paddy rice, denaturing gradient gel electrophoresis (DGGE) analysis targeting 18S rDNA followed by sequencing was conducted in a Japanese paddy field. The eukaryotic communities in rice straw compost incorporated into the flooded paddy field were influenced by the mid-season drainage and mainly composed of fungi (Ascomycota, Zygomycota, and Chytridiomycota) and protozoa (Ciliophora, Euglyphida, and Dactylopodida), most of which existed continuously during the cultivation period of paddy rice. The results indicated that these eukaryotic members were associated with the decomposition of rice straw compost in paddy field soil directly or indirectly

  61. *Selective grazing of methanotrophs by protozoa in a rice field soil. Reviewed

    Murase,J. Frenzel,P.

    FEMS Microbiol. Ecol.   Vol. 65   page: 408-414   2008

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  62. Phyologenetic positions of Mn-oxidizing bacteria and fungi inhabiting Mn nodules in rice field subsoils. Reviewed

    Vita Ratri Cahyani, Jun Murase, Akihiro Ikeda, Katsutoshi Taki, Susumu Asakawa, and Makoto Kimura

    Bio. Fert. Soils     page: 10.1007/s00374-008-0337-8.   2008

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  63. Bacterial communities in Fe mottles in the plow pan layer of a Japanese rice field: estimation using PCR-DGGE and sequencing analyses Reviewed

    Vita Ratri Cahyani, Jun Murase, Eiji Ishibasi, Susumu Asakawa, and Makoto Kimura

    Soil Sci. Plant Nutr.   Vol. 54 ( 4 ) page: doi: 10.1111/j.1747-0765.2008.00291.x   2008

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  64. *A methane-driven microbial food web in a wetland rice soil. Reviewed

    Murase, J., Frenzel,P.

    Environ. Microbiol.   Vol. 9 ( 12 ) page: 3025-3034   2007.12

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    Methane oxidation is a key process controlling methane emission from anoxic habitats into the atmosphere. Methanotrophs, responsible for aerobic methane oxidation, do not only oxidize but also assimilate methane. Once assimilated, methane carbon may be utilized by other organisms. Here we report on a microbial food web in a rice field soil driven by methane. A thin layer of water-saturated rice field soil was incubated under opposing gradients of oxygen and 13C-labelled methane. Bacterial and eukaryotic communities incorporating methane carbon were analysed by RNA-stable isotope probing (SIP). Terminal restriction fragment length polymorphism (T-RFLP) and cloning showed that methanotrophs were the most prominent group of bacteria incorporating methane carbon. In addition, a few Myxobacteria-related sequences were obtained from the heavy rRNA fraction. Denaturing gradient gel electrophoresis (DGGE) targeting 18S rRNA detected various groups of protists in the heavy rRNA fraction including naked amoeba (Lobosea and Heterolobosea), ciliates (Colpodea) and flagellates (Cercozoa). Incubation of soil under different methane concentrations in air resulted in the development of distinct protozoan communities. These results suggest that methane carbon is incorporated into non-methanotrophic pro- and microeukaryotes probably via grazing, and that methane oxidation is a shaping force of the microeukaryotic community depending on methane availability

  65. *水田土壌の細菌群集の構造と機能に及ぼす原生動物の影響 Invited

    村瀬 潤

    土と微生物   Vol. 61   page: 65-68   2007

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  66. Bacterial communities in manganese nodules in rice field subsoils: estimation by PCR-DGGE and sequencing analyses. Reviewed

    Cahyani, V.R., Murase,J., Ishibashi,E., Asakawa,S., Kimura,M.

    Soil Sci. Plant Nutr.   Vol. 53   page: 578-584   2007

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    The phylogenetic positions of bacterial communities in manganese (Mn) nodules from subsoils of two Japanese rice fields were estimated using polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) analysis followed by sequencing of 16S rDNA. The DGGE band patterns and sequencing analysis of characteristic DGGE bands revealed that the bacterial communities in Mn nodules were markedly different from those in the plow layer and subsoils. Three out of four common bands found in Mn nodules from two sites corresponded to Deltaproteobacteria and were characterized as sulfate-reducing and iron-reducing bacteria. The other DGGE bands of Mn nodules corresponded to sulfate and iron reducers (Deltaproteobacteria), methane-oxidizing bacteria (Gamma and Alphaproteobacteria), nitrite-oxidizing bacteria (Nitrospirae) and Actinobacteria. In addition, some DGGE bands of Mn nodules showed no clear affiliation to any known bacteria. The present study indicates that members involved in the reduction of Mn nodules dominate the bacterial communities in Mn nodules in rice field subsoils

  67. Impact of protists on the activity and structure of the bacterialcommunity in a rice field soil Reviewed

    Murase, J., Noll, M., Frenzel,P.

    Applied and Environmental Microbiology   Vol. 72 ( 8 ) page: 5436-5444   2006.8

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    Flooded rice fields have become a model system for the study of soil microbial ecology. In Italian rice fields,
    in particular, aspects from biogeochemistry to molecular ecology have been studied, but the impact of protistan
    grazing on the structure and function of the prokaryotic community has not been examined yet. We compared
    an untreated control soil with a -radiation-sterilized soil that had been reinoculated with a natural bacterial
    assemblage. In order to verify that the observed effects were due to protistan grazing and did not result from
    sterilization, we set up a third set of microcosms containing sterilized soil that had been reinoculated with
    natural assemblage bacteria plus protists. The spatial and temporal changes in the protistan and prokaryotic
    communities were examined by denaturing gradient gel electrophoresis (DGGE) and terminal restriction
    fragment length polymorphism (T-RFLP) analysis, respectively, both based on the small-subunit gene. Sequences
    retrieved from DGGE bands were preferentially affiliated with Cercozoa and other bacteriovorous
    flagellates. Without protists, the level of total DNA increased with incubation time, indicating that the level of
    the microbial biomass was elevated. Betaproteobacteria were preferentially preyed upon, while low-GC-content
    gram-positive bacteria became more dominant under grazing pressure. The bacterial diversity detectable by
    T-RFLP analysis was greater in the presence of protists. The level of extractable NH4
    was lower and the level
    of extractable SO4
    2 was higher without protists, indicating that nitrogen mineralization and SO4
    2 reduction
    were stimulated by protists. Most of these effects were more obvious in the partially oxic surface layer (0 to 3
    mm), but they could also be detected in the anoxic subsurface layer (10 to 13 mm). Our observations fit well
    into the overall framework developed for protistan grazing, but with some modifications pertinent to the
    wetland situation

  68. 土壌中の遺伝子・遺伝情報 何ができるのか、何が分かるのか9.土壌DNAの利用、新しい研究手法(その2)Stable Isotope Probing Invited Reviewed

    村瀬 潤

    日本土壌肥料学雑誌   Vol. 77 ( 2 ) page: 231-234   2006

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  69. *Incorporation of 13C-labeled rice straw carbon into microbial communities in submerged rice field soil and the percolating water. Reviewed

      Vol. 38   page: 3483-3491   2006

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  70. Vertical Changes in Bacterial communities in percolating water of a Japanese paddy field as revealed by PCR-DGGE. Reviewed

    Murase,J., Shimizu,M., Hayashi,M., Matsuya,K., and Kimura,M.

    Soil Sci. Plant Nutr.   Vol. 51   page: 821-829   2005

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  71. Carbon cycling in rice field ecosystems in the context of input, decomposition and translocation of organic materials and the fates of their end products (CO2 and CH4). Reviewed

    Kimura,M., Murase,J., and Lu,Y.

    Soil Biol. Biochem.   Vol. 36   page: 1399-1416   2005

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  72. Effect of rice straw amendment on dissolved organic and inorganic carbon, and cationic nutrients in percolating water from a flooded paddy soil: A microcosm experiment using 13C-enriched rice straw. Invited Reviewed

    Katoh,M., Murase,J., Sugimoto,A., and Kimura,M.

    Org. Geochem.   Vol. 36   page: 803-811   2005

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  73. Bacterial community responsible for the decomposition of rice straw compost in a Japanese rice paddy field estimated by DGGE analysis of amplified 16S rDNA and 16S rRNA fragments. Reviewed

    Tanahashi,T., Murase,J., Matsuya,K., Asakawa, S., and Kimura,M.

    Soil Sci. Plant Nutr., 51, 351-360 (2005)   Vol. 51   page: 351-360   2005

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  74. Dynamics of methane in a mesotrophic Lake Biwa, Japan. Invited Reviewed

    Murase.J., Sakai,Y., Kametani,A., and Sugimoto,A.

    Ecol. Res.   Vol. 20   page: 377-385   2005

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  75. Inhibitory effect of light on methane oxidation in the pelagic water column of a mesotrophic lake (Lake Biwa, Japan). Reviewed

    Murase,J. and Sugimoto,A.

    Limnol. Oceanogr.   Vol. 50   page: 1339-1343   2005

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    Methane oxidation was studied in mesotrophic lake water (Lake Biwa, Japan) under thermally stratified conditions. Methane oxidation rates at in situ concentrations were very low in lake water from the epilimnion and thermocline but were high in hypolimnetic water. Incubation under light conditions ranging from 4.1 to 57 mu mol photons m(-2) s(-1) resulted in decreased methane oxidation in hypolimnetic water. This inhibition was more severe as the light intensity increased. Addition of inorganic nitrogen (ammonium and nitrate) did not promote methane oxidation in the thermocline but inhibited it in the hypolimnion. Methane oxidation activity in the thermocline was observed after I month of incubation under dark conditions. Our results suggest that the inhibitory effect of light was bacteriostatic for the methanotrophic population. The different rates of methane oxidation between the hypolimnion and epilimnion/thermocline may explain the surface maximum of dissolved methane during the period of thermal stratification

  76. Microbial communities responsible for the decomposition of rice straw compost in a Japanese rice paddy field determined by phospholipid fatty acid (PLFA) analysis. Reviewed

    Tanahashi,T., Murase,J., Matsuya,K., Asakawa,S., and Kimura,M.

    Soil Sci. Plant Nutr., 50, 1229-1236 (2004)   Vol. 50   page: 1229-1236   2004

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  77. Sites and process of nutrient accumulation in the subsoil through water percolation from the plow layer in a submerged paddy soil microcosm. Reviewed

    Katoh,M., Murase,J., and Kimura,M.

    Soil Sci. Plant Nutr.   Vol. 50   page: 731-738   2004

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  78. Nutrient leaching from the plow layer by water percolation and accumulation in the subsoil in an irrigated paddy field. Reviewed

    Katoh,M., Murase,J., Hayashi,M., Matsuya,K., and Kimura,M.

    Soil Sci. Plant Nutr.   Vol. 50   page: 721-729   2004

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  79. Biotic and abiotic methane releases from Lake Biwa sediment slurry. Reviewed

    Dan,J., Kumai,T., Sugimoto,A., and Murase,J.

    Limnology   Vol. 5   page: 149-154   2004

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  80. Linking microbial community dynamics to rhizosphere carbon flow in a wetland rice soil. Reviewed

    Lu,Y., Murase,J., Watanabe,A., Sugimoto,A., and Kimura,M.

    FEMS Microbiol. Ecol.   Vol. 48   page: 179-186   2004

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  81. Analysis of communities of ammonia oxidizers, methanotrophs, and methanogens associated with microcrustaceans in the floodwater of a rice field microcosm. Reviewed

    Niswati,A., Murase,J., Asakawa,S., and Kimura,M.

    Soil Sci. Plant Nutr.   Vol. 50   page: 447-455   2004

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  82. Effect of application of rice straw and compost on the community structure of bacteria associated with microcrustaceans in the floodwater of a paddy field microcosm. Reviewed

    Niswati,A., Murase,J., and Kimura,M.

    Soil Sci. Plant Nutr.   Vol. 49   page: 191-200   2003

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  83. Adsorption as a methane storage process in natural lake sediment. Reviewed

    Sugimoto,A., Dan,J., Kumai,T., and Murase,J.

    Geophys. Res. Lett.   Vol. 30   page: doi:10.1029/2003GL018162   2003

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  84. Molecular analysis of β-proteobacterial ammonia oxidizer populations in surface layers of a submerged paddy soil microcosm. Reviewed

    Murase,J., Itoh,K., Kano,M., and Kimura,M.

    Soil Sci. Plant Nutr.   Vol. 49   page: 909-913   2003

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  85. Sources of dissolved methane in Lake Biwa. Reviewed

    Murase,J., Sakai,Y., Sugimoto,A., Okubo,K., and Sakamoto,M.

    Limnology   Vol. 4   page: 91-99   2003

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  86. 第2編第2章2.2自然特性

    村瀬潤

        2002.3

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    流域管理のための総合調査マニュアル

  87. Resuspension of bottom sediment by an internal wave in Lake Biwa Reviewed

    Sakai,Y., Murase,J., Sugimoto,A., Okubo,K., and Nakayama,E.

    Lakes Reserv.   Vol. 7   page: 339-344   2002

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  88. Seasonal and spatial variations of methane production in mesotrophic lake sediments (Lake Biwa, Japan).

    Murase,J. and Sugimoto,A.:

    Verh.Int. Ver. Limnol.   Vol. 28   page: 971-974   2002

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  89. Comparison of microbial communities in percolating water from plow layer and subsoil layer estimated by phospholipid fatty acid (PLFA) analysis. Reviewed

    Shimizu,M., Hayashi,M., Matsuya,K., Murase,J., and Kimura,M.

    Soil Sci. Plant Nutr.   Vol. 48   page: 877-881   2002

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  90. Uptake of carbon and nitrogen through roots of rice and corn plants, grown in soils treated with 13C and 15N dual-labeled cattle manure compost. Reviewed

    Yamamuro,S., Ueno,H., Yamada,H., Takahashi,Y., Shiga,Y., Miyahara,S., Shimonihara,T., Murase,J., Yanai,J., and Nishida,M.

    Soil Sci. Plant Nutr.   Vol. 48   page: 787-795   2002

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  91. 沿岸帯を観るキーワード -その多面的多様性- Invited Reviewed

    村瀬 潤・野崎健太郎・山田佳裕

    陸水学雑誌   Vol. 63   page: 269-270   2002

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  92. Spatial distribution of methane in the Lake Biwa sediments and its carbon isotopic compositions

    Jun MURASE & Atsuko SUGIMOTO

    Geochemical Journal   Vol. 35   page: 257-263   2001.8

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  93. Horizontal distribution of carbon and nitrogen and their isotopic compositions in the surface sediment of Lake Biwa

    "Murase,J. and Sakamoto,M"

    Limnology   Vol. 1   page: 177-184   2000.1

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  94. 琵琶湖堆積物の生元素の空間的分布

    村瀬潤

        1998.1

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    "エコフロンティア1, 52-55"

  95. "Anaerobic reoxidation of Mn2+, Fe2+, S0 and S2- in submerged paddy soils."

    "Murase,J. and Kimura,M.:"

    Biol.Fert.Soil.   Vol. 25   page: 302-306   1997.1

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  96. Anaerobic reoxidation of Mn2+, Fe2+, S0 and S2- in submerged poddy soils.

    Biology Fertility Soil   Vol. 25   page: 302-306   1997

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  97. Methane production and its fate in paddy fields. IX. Methane flux distribution and decomposition of methane in the subsoil during the growth period of rice plants.

    "Murase,J. and Kimura,M."

    Soil Sci. Plant Nutr.   Vol. 42(1)   page: 187-190   1996.1

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  98. Utilization of the feces sludge from the swine farm for rice production in the problem soils in Thailand.

    "Murase,J., Korpraditskul,R., Osotsapar,Y., and Korpraditskul,V"

    J. ISSAAS   Vol. 2   page: 54-71   1996.1

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  99. Methane production and its fate in paddy fields. (]G0009[) Methane flux distribution and decompostition of methane in the subsoil during the growth period of rice plants

    Soil Science and Plant Nutrition   Vol. 42 ( 1 ) page: 187-190   1996

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  100. Utilization of the faces sludge from the swine Farm for rice production in the problem soils in Thailand

    Journal of International Society for Southeast Asian Agricultural Sciences(J. ISSAAS)   Vol. 2   page: 54-71   1996

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  101. Methane production and its fate in paddy fields. IV. Sources of microorganisms and substrates responsible for anaerobic methane oxidation in subsoil. Reviewed

    Soil Science and Plant Nutrition   Vol. 40 ( 1 ) page: 57-61   1994

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  102. Methane production and its fate in paddy fields. (]G0006[) Anaerobic oxidation of methane in plow layer soil

    Soil Science and Plant Nutrition   Vol. 40 ( 3 ) page: 505-514   1994

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  103. Methane production and Its fate in paddy fields. (]G0007[) Electron Acceptors responsible for anaerobic methane oxidation

    Soil Science and Plant Nutrition   Vol. 40 ( 4 ) page: 647-654   1994

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  104. Methane production and its fate in paddy fields. (]G0003[). Effects of percolation on methane flux distribution to the atmosphere and the subsoil

    Soil Science and Plant Nutrition   Vol. 39 ( 1 ) page: 63-70   1993

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Books 19

  1. Methane emissions in livestock and rice systems – Sources, quantification, mitigation and metrics Reviewed

    FAO( Role: Joint editor)

    FAO  2023.9  ( ISBN:978-92-5-138148-9

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    Total pages:313   Language:English Book type:Textbook, survey, introduction

  2. 原生生物学事典

    村瀬 潤( Role: Contributor ,  土壌原生生物)

    朝倉書店  2023.5  ( ISBN:978-4-254-17181-5

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    Total pages:446   Responsible for pages:334-337   Language:Japanese Book type:Dictionary, encyclopedia

  3. 原生生物学事典

    村瀬 潤、松原 立真、桑田 晃、神山 孝史( Role: Contributor ,  農業・畜産業・水産業)

    朝倉書店  2023.5  ( ISBN:978-4-254-17181-5

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    Total pages:446   Responsible for pages:358-361   Language:Japanese Book type:Dictionary, encyclopedia

  4. 身近な水の環境科学第2版

    村瀬 潤( Role: Contributor ,  水田の物質循環)

    朝倉書店  2022.4  ( ISBN:978-4-254-18062-6

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    Total pages:154   Responsible for pages:76-79   Language:Japanese Book type:Scholarly book

  5. 水田微生物の特徴と生産性とのかかわり

    村瀬 潤( Role: Sole author)

    朝倉書店  2018.8  ( ISBN:978-4-254-43571-9

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    Language:Japanese Book type:Scholarly book

  6. 最新農業技術「土壌施肥 vol.9」

    村瀬 潤( Role: Sole author ,  太陽熱処理が土壌繊毛虫群集に及ぼす影響)

    農文協  2017.3 

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    Language:Japanese Book type:Scholarly book

  7. 土のひみつ-食料・環境・生命-

    日本土壌肥料学会「土のひみつ」編集グループ(編)( Role: Joint author)

    朝倉書店  2015.9 

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    Language:Japanese

  8. 4.1 微生物.身近な水の環境科学-実習・測定編-

    日本陸水学会東海支部会( Role: Joint author)

    朝倉書店  2014.6 

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    Language:Japanese

  9. 第16章 Stable Isotope Probing、土壌微生物実験法第3版

    日本土壌微生物学会 (編)( Role: Sole author)

    養賢堂  2013.6 

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    Language:Japanese

  10. 第10章 原生動物 (10-2~10-6節)、土壌微生物実験法第3版

    日本土壌微生物学会 (編)( Role: Sole author)

    養賢堂  2013.6 

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    Language:Japanese

  11. 12. 窒素サイクル,メタンサイクルに果たす微生物の役割、 シリーズ 現代の生態学11巻

    村瀬 潤( Role: Sole author)

    共立出版  2011.3 

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    Language:Japanese

  12. 7.水田の土壌環境と微生物相  身近な自然の保全生態学

    村瀬 潤, 浅川 晋( Role: Joint author)

    培風館  2010.10 

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    Language:Japanese

  13. 世紀を超えて座談会を傍聴する、日本微生物生態学会編、土壌微生物通信(1962-1986)探訪(2) 「通信」の時代と現在の間

    村瀬 潤( Role: Sole author)

    博友社  2010.5 

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    Language:Japanese

  14. 7.集水域の中の水田、日本陸水学会東海支部会編、身近な水の環境科学-源流から干潟まで-

    村瀬 潤( Role: Sole author)

    朝倉書店  2010.1  ( ISBN:978-4-254-18023-7

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    村瀬 潤:7.集水域の中の水田、日本陸水学会東海支部会編、身近な水の環境科学-源流から干潟まで-、p.92-102、朝倉書店(2010)

  15. *土壌の原生生物・線虫群集-その土壌生態系での役割-

    橋本知義、村瀬 潤、島野智之、岡田浩明、豊田剛己、佐藤恵利華( Role: Joint author)

    博友社  2009.8 

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  16. Terrestrial ecosystems in a changing world.

    Kohyama,T., Urabe,J., Hikosaka,K., Shibata,H., Yoshioka,T., Konohira,E., Murase,J., Wada,E.( Role: Joint author)

    Springer  2007 

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    Terrestrial ecosystems in monsoon Asia: scaling up from shoot module to watershed.

  17. 地球環境と生態系 5.4 水系のガス代謝

    村瀬 潤・杉本敦子( Role: Joint author)

    共立出版  2006.5 

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    Language:Japanese

  18. 東アジアモンスーン域の湖沼と流域 3-4湖の富栄養化の現状、変動経過、原因

    坂本 充・村瀬 潤・丸尾雅啓( Role: Joint author)

    名古屋大学出版会  2006 

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  19. 新・土の微生物 (5)系統分類からみた土の細菌

    村瀬潤( 編:日本土壌微生物学会編)( Role: Joint author)

    博友社  2000 

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    2-7δおよびγプロテオバクテリア

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Presentations 59

  1. 水稲根圏微生物叢の形成に及ぼす水稲品種、土壌タイプ、施肥の影響

    天野 真宏, 長谷川 昂平, 森 拓也, 西内 俊策, 土井 一行, 勝見尚也, Rasit ASILOGLU, 高橋 宏和, 中園 幹生, 村瀬 潤

    日本微生物生態学会第35回大会  2022.11  日本微生物生態学会

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    Event date: 2022.11

    Language:Japanese   Presentation type:Poster presentation  

    Venue:札幌   Country:Japan  

  2. トウモロコシへの耐湿性遺伝子の導入が根圏微生物叢に及ぼす影響

    村瀬 潤、間野吉郎、大森史恵、高橋秀和、高橋宏和、中園幹生

    日本土壌肥料学会2022年度東京大会  2022.9  日本土壌肥料学会

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    Event date: 2022.9

    Language:Japanese   Presentation type:Poster presentation  

    Venue:東京   Country:Japan  

  3. 異なる水稲栽培品種の根圏微生物群集の施肥に対する応答

    天野 真宏、長谷川 昂平、森 拓也、西内 俊策、土井 一行、吉田 英樹、松岡 信、高橋 宏和、中園 幹生、村瀬 潤

    日本土壌肥料学会2022年度東京大会  2022.9  日本土壌肥料学会

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    Event date: 2022.9

    Language:Japanese   Presentation type:Poster presentation  

    Venue:東京   Country:Japan  

  4. 水田土壌表層の微生物群集に及ぼす光の影響

    出水明日香、村瀬潤

    日本土壌微生物学会2022 年度鹿児島大会  2022.6  日本土壌微生物学会

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    Event date: 2022.6

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:鹿児島   Country:Japan  

  5. 異なる水稲栽培品種の根圏微生物群集の施肥に対する応答

    天野真宏, 長谷川昂平, 森拓也, 西内俊策, 土井一行, 松岡信, 高橋宏和, 中園幹生, 村瀬潤

    日本土壌肥料学会2021年度北海道大会  2021.9  日本土壌肥料学会

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    Event date: 2021.9

    Language:Japanese   Presentation type:Poster presentation  

    Venue:北海道  

  6. Microeukaryotic community in the upland soil under the long-term fertilizer treatments.

    Sok, S., and Murase, J.

    日本土壌肥料学会2020年度倉敷大会  2020.9  日本土壌肥料学会

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    Event date: 2020.9

    Language:English   Presentation type:Poster presentation  

    Venue:岡山  

  7. 温度は細菌を捕食する土壌原生生物の群集構成を支配する International coauthorship

    大島崇彰, Gillar, O., 村瀬潤

    日本土壌肥料学会中部支部2019年名古屋大会  2019.11.27  日本土壌肥料学会中部支部

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    Event date: 2019.11

    Language:Japanese   Presentation type:Poster presentation  

    Venue:名古屋  

  8. 土壌に導入された微生物を利用する微生物群集のStable Isotope Probing法による解析 International coauthorship

    南川浩樹, 大島崇彰, Gillar, O., 村瀬潤

    日本土壌肥料学会2019年度静岡大会  2019.9.3  日本土壌肥料学会

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    Event date: 2019.9

    Language:Japanese   Presentation type:Poster presentation  

    Venue:静岡  

  9. 水稲根圏の細菌群集に及ぼす原生生物の影響

    永井裕也、村瀬 潤

    日本土壌微生物学会2019年度大会  2019.6.15  日本土壌微生物学会

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    Event date: 2019.6

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:北海道  

  10. 田んぼの中の原生生物たちの暮らし Invited

    村瀬 潤

    日本土壌微生物学会2019年度大会  2019.6.16  日本土壌微生物学会

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    Event date: 2019.6

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Venue:北海道  

  11. 湛水水田土壌表層における溶存酸素鉛直プロファイルの昼夜変動

    村瀬潤, 後藤佳介, 杉浦大輔

    日本土壌肥料学会中部支部2018年度大会  2018.11.29  日本土壌肥料学会中部支部

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    Event date: 2018.11

    Language:Japanese   Presentation type:Poster presentation  

    Venue:静岡  

  12. 土壌繊毛虫群集に及ぼす温度環境の影響-高温に対する脆弱性と復元力

    大島崇彰、篠原陽子、村瀬 潤

    日本土壌肥料学会2018年度神奈川大会  2018.8.29  日本土壌肥料学会

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    Event date: 2018.8

    Language:Japanese   Presentation type:Poster presentation  

    Venue:藤沢  

  13. The effect of humic substances on the methanogenic microbial community in the artificial rice field soil International conference

    Maeda, Y., and Murase, J.

    17th International Symposium on Microbial Ecology  2018.8  International Society of Microbial Ecology

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    Event date: 2018.8

    Language:English   Presentation type:Poster presentation  

    Venue:Leipzig   Country:Germany  

  14. Microflora in Arctic Wetland Soils of a Taiga-Tundra Ecotone in Northeastern Siberia International coauthorship International conference

    Murase, J., Sugimoto, A., Shingubara, R., and Maximov, T. C.

    The 10th Asian Symposium in Microbial Ecology  2018.7 

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    Event date: 2018.7

    Language:English   Presentation type:Poster presentation  

  15. 長期施肥試験圃場で生育する作物の根圏微生物群集-発病抑止性との関連-

    村瀬 潤、田原保樹、日置真優、西内俊策、北野英己、柘植尚志

    日本土壌微生物学会2018年度大会  2018.6.16  日本土壌微生物学会

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    Event date: 2018.6

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:西条  

  16. Ecology of protists in a rice rhizosphere Invited International conference

    International Workshop of Mollisols Ecology  2018.6.8  Chinese Academy of Science

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    Event date: 2018.6

    Language:English   Presentation type:Oral presentation (invited, special)  

    Venue:Harbin  

  17. Methane-driven microbial food web in the rice rhizosphere International conference

    Hibino, Y., Tokida, T., Okubo, T., Arai, M., Hayashi, K., Sakai, H., Hasegawa, T., and Murase, J.

    13 th International Conference of The East and Southeast Asia Federation Of Soil Sciences (13 th ESAFS)  2017.12  East and Southeast Asia Federation Of Soil Sciences

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    Event date: 2017.12

    Language:English   Presentation type:Poster presentation  

    Venue:Pattaya   Country:Thailand  

  18. 水稲根圏における原生生物の生態と機能 Invited

    村瀬 潤

    第362回生存権シンポジウム  2017.11.21  京都大学

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    Event date: 2017.11

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Venue:宇治  

  19. 北東シベリア湿地土壌のメタン酸化のポテンシャルと微生物叢の特性 International coauthorship

    村瀬潤, 杉本敦子, 新宮原諒, マキシモフ・トロフィーム・C

    日本土壌肥料学会中部支部2017年度大会  2017.10.26  日本土壌肥料学会中部支部

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    Event date: 2017.10

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:富山  

  20. 水稲根圏におけるメタン酸化食物連鎖の安定同位体プロービング法による解析

    日比野優子, 常田岳志, 大久保卓, 荒井美和, 林健太郎, 酒井英光, 長谷川利拡, 村瀬潤

    日本土壌肥料学会2017年度仙台大会  2017.9  日本土壌肥料学会

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    Event date: 2017.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:仙台  

  21. ⽔⽥⼟壌のメタン酸化微⽣物⾷物連鎖の構造と機能 International conference

    村瀬潤, 日比野優子, 常田岳志, 大久保卓, 荒井美和, 林健太郎, 酒井英光, 長谷川利拡

    日本地球惑星科学連合2017年大会  2017.5.24  日本地球惑星科学連合

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    Event date: 2017.5

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:千葉  

  22. 人工水田土壌のメタン生成活性と微生物群集に及ぼす土壌構成要素の影響

    村瀬潤, 前田悠

    日本微生物生態学会第31回大会  2016.10  日本微生物生態学会

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    Event date: 2016.10

    Language:Japanese   Presentation type:Poster presentation  

    Venue:横須賀  

  23. Diversity and ecology of microeukaryotes in rice field soil Invited International conference

    The 8th Asian Symposium on Microbial Ecology  2016.10.1  Taiwan Society of Microbial Ecology

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    Event date: 2016.9 - 2016.10

    Language:English   Presentation type:Oral presentation (invited, special)  

    Country:Taiwan, Province of China  

  24. 北東シベリア湿地土壌のメタン酸化ポテンシャル International coauthorship

    村瀬潤, 杉本敦子, 新宮原諒, マキシモフ・トロフィーム・C

    日本土壌肥料学会2016年度佐賀大会  2016.9.20  日本土壌肥料学会

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    Event date: 2016.9

    Language:Japanese   Presentation type:Poster presentation  

    Venue:佐賀  

  25. Response of the protistan community of a rice field soil to different oxygen tensions. International conference

    16th International Symposium on Microbial Ecology  2016.8  International Society of Microbial Ecology

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    Event date: 2016.8

    Language:English   Presentation type:Poster presentation  

    Venue:Montreal   Country:Canada  

  26. 太陽熱土壌消毒が土壌微生物群集に及ぼす影響のeDNA解析による評価

    大島崇彰, 篠原陽子, 山野明日夏, 前坂昌宏, 横江和典, 山縣美奈, 橋本知義, 村瀬潤, 浅川晋

    日本土壌微生物学会2016年度大会  2016.6.11  日本土壌微生物学会

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    Event date: 2016.6

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:岐阜  

  27. 有機物資材の長期施用が畑土壌の病害抑止性と微生物群集に及ぼす影響

    村瀬潤, 田原保樹, 日置真優, 西内俊策, 北野英己, 柘植尚志

    日本土壌微生物学会2016年度大会  2016.6.11  日本土壌微生物学会

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    Event date: 2016.6

    Language:Japanese   Presentation type:Poster presentation  

    Venue:岐阜  

  28. Methane Oxidation Potential of Arctic Wetland Soil of a Taiga-Tundra Ecotone in Northeastern Siberia International coauthorship International conference

    2016.5.26 

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    Event date: 2016.5

    Language:English   Presentation type:Poster presentation  

  29. 水田土壌微生物の態解析を目指した人工開発 —腐植物質が微生群集およびその活性に及ぼす影響 —

    前田悠, 村瀬潤

    日本土壌肥料学会中部支部第95回例会  2016.3.3  日本土壌肥料学会中部支部

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    Event date: 2016.3

    Language:Japanese   Presentation type:Poster presentation  

    Venue:三重  

  30. 水田土壌微生物の生態解析を目指した人工土壌の開発

    前田悠, 村瀬潤

    第31回日本腐植物質学会  2015.11.20  日本腐植物質学会

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    Event date: 2015.11

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:名古屋  

  31. Methane oxidation potential of arctic wetland soil of a Taiga-Tundra ecotone in Northeastern Siberia. International coauthorship International conference

    Murase, J., Sugimoto, A., Shingubara, R., and Maximov, T. C

    The 7th Japan-Taiwan-Korea International Symposium on Microbial Ecology  2015.10.18 

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    Event date: 2015.10

    Language:English   Presentation type:Poster presentation  

  32. Methane-driven microbial food web in a rice field soil Invited International conference

    International Synposiumm "From classic to Frontiers: Methanotrophy and Meta-omics”  2015.9.21  Chinese Academy of Science

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    Event date: 2015.9

    Language:English   Presentation type:Oral presentation (invited, special)  

    Country:China  

  33. Diversity and Ecology of Microeukaryotic Community in a Paddy Soil International conference

    12th International Conference of the East and Southeast Asia Federation of Soil Science Societies (ESAFS2015)  2015.9.18  East and Southeast Asia Federation of Soil Science Societies

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    Event date: 2015.9

    Language:English  

    Country:China  

  34. 異なる有機物資材の長期施用が畑土壌の細菌群集に及ぼす影響

    村瀬潤, 田原保樹, 日置真優, 西内俊策, 北野英己, 柘植尚志

    日本土壌肥料学会2015年京都大会  2015.9  日本土壌肥料学会

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    Event date: 2015.9

    Language:Japanese   Presentation type:Poster presentation  

    Venue:京都  

  35. Methane Oxidation Potential of Arctic Wetland Soil of a Taiga-Tundra Ecotone in Northeastern Siberia International coauthorship International conference

    2015.5.26 

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    Event date: 2015.5

    Language:English   Presentation type:Poster presentation  

  36. Assimilation of Plant-derived Carbon by Microeukaryotes in a Rice Field Soil International conference

    Murase, J., Lee, C. G., Li, Y., Shibata, M., Nakatani, H., Minami, H., Watanabe, T., Asakawa, S., and Kimura, M.

    15th International Symposium on Microbial Ecology  2018.8  International Society of Microbial Ecology

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    Event date: 2014.8

    Language:English   Presentation type:Poster presentation  

    Country:Korea, Republic of  

  37. Assimilation of Plant-derived Carbon by Microeukayotes in Rice Field Soil Revealed Using Stable-Isotope Probing. International conference

    Murase, J., Lee, C. G., Li, Y., Shibata, M., Nakatani, H., Minami, H., Watanabe, T., Asakawa, S., and Kimura, M.

    5th TKJ International Symposium in Microbial Ecology  2013.11.1  Taiwan Society of Microbial Ecology

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    Event date: 2013.11

    Language:English   Presentation type:Poster presentation  

    Country:Taiwan, Province of China  

  38. 長期の施肥管理が水田土壌の繊毛虫群集に及ぼす影響

    村瀬潤, 松田梨里, 飛田あづさ, 小川佳織, 今井克彦, 吉川那々子, 野々山利博, 木村眞人

    日本土壌肥料学会2013年度名古屋大会  2013.9  日本土壌肥料学会

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    Event date: 2013.9

    Language:Japanese   Presentation type:Poster presentation  

    Venue:名古屋  

  39. PLFA-SIP法を用いた水田土壌の原生動物によるメタン酸化細菌の捕食解析

    村瀬 潤、Paul Bodelier

    日本土壌微生物学会 

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    Event date: 2010.5

    Language:Japanese   Presentation type:Poster presentation  

    Country:Japan  

  40. 微生物食物連鎖の分子生物学的解析:メタン酸化を例として

    日本生態学会 

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    Event date: 2010.3

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  41. 水田土壌から分離された新奇Heteroloboseaアメーバの形態学的・分子系統学的解析

    村瀬 潤、Johan F. De Jonckheere

    日本陸水学会東海支部会 

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    Event date: 2010.2

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  42. 原生動物の捕食作用が水田土壌のメタン酸化細菌群集に及ぼす影響

    村瀬 潤、Peter Frenzel

    日本微生物生態学会 

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    Event date: 2009.11

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  43. 有機物施用および酸素の有無が水田土壌の真核微生物群集に及ぼす影響

    岩崎和史・村瀬 潤・木村眞人

    日本土壌肥料学会 

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    Event date: 2009.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  44. Impact of protozoan grazing on the community of methanotrophic bacteria in a wetland rice field soil International conference

    the10th InternationalSymposium on Bacterial Genetics and Ecology 

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    Event date: 2009.6

    Language:English   Presentation type:Poster presentation  

  45. 水田土壌のメタン酸化細菌群集におよぼす原生動物の影響

    村瀬 潤・Peter Frenzel

    日本土壌微生物学会 

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    Event date: 2009.6

    Language:Japanese   Presentation type:Poster presentation  

    Country:Japan  

  46. 水田土壌の原生動物によるメタン酸化細菌の選択的捕食

    村瀬 潤、Paul L.E. Bodelier

    日本陸水学会東海支部会 

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    Event date: 2009.2

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  47. 水田土壌生態系の機能における細菌-原生生物系の役割

    日本土壌肥料学会2008年度大会 

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    Event date: 2008.9

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  48. Selective grazing of free-living naked amoeba on methane-oxidizing bacteria. (Wako, Japan) 9 2007.8-27-30 International conference

    XIIth International Meeting on the Biology and Pathogenicity of Free-Living Amoebae 

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    Event date: 2007.8

    Language:English   Presentation type:Poster presentation  

    Country:Japan  

    Biological methane oxidation at the oxic-anoxic interface in wetland soils and sediments is a key process in methane cycle, preventing large amounts of this greenhouse gas from escaping into the atmosphere. Methane oxidizing bacteria (MOB) is the only group of aerobic bacteria that oxidize methane and assimilate methane-C. However, the fate of MOB-biomass is largely unknown. Here we demonstrate that a microbial food web can be driven by methane. Soil microcosms, in which a thin layer of water-sa

  49. 水田土壌から単離したアメーバの系統解析

    村瀬 潤・Peter Frenzel

    日本土壌肥料学会2007年度大会 

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    Event date: 2007.8

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

    原生動物は細菌群集の構造や機能に影響を及ぼす生物因子である。土壌原生動物の生残・活性は土壌の水分状態に支配されるので、湛水状態の水田土壌は原生動物にとって好適な環境であると予想される。水田土壌中の原生動物の研究は、これまで繊毛虫が主たる対象であり、細菌に対する影響が大きいといわれるアメーバ、鞭毛虫の多様性に関する知見は限られている。本研究では、水田土壌から単離したアメーバについて18SrRNA遺伝子配列に基づく系統解析を行った。

  50. メタン酸化細菌に対する原生動物の捕食選択性

    村瀬 潤・Peter Frenzel

    日本土壌微生物学会2007年度大会 

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    Event date: 2007.6

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

    水田土壌の嫌気層で生成したメタンは,大気へと放出される過程で土壌表層や好気的な根圏で大部分酸化されると考えられている。これまでに,酸化還元層を再現したモデル実験により,メタン酸化細菌以外にも細菌捕食性の原生動物がメタン由来の炭素を同化することが明らかとなり,原生動物によるメタン酸化細菌バイオマスの捕食作用が示唆された。本研究では,異なるメタン酸化細菌に対する土壌原生動物の捕食性を比較した。
    メタンを基質として培養したメタン酸化細菌10株を餌細菌に含む無機塩培地を用いて,水田土壌中の原生動物数を最

  51. The Methanotrophic Microbial Food Chain in a Rice Field Soil International conference

    The 10th International Symposium on Wetland Biogeochemistry 

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    Event date: 2007.4

    Language:English   Presentation type:Oral presentation (invited, special)  

    Biological methane oxidation at the oxic-anoxic interface in wetland soils and sediments is a key process in methane cycle, preventing large amounts of this greenhouse gas to escape into the atmosphere. A specific group of aerobic bacteria oxidises methane and is also able to assimilate methane-C. In limnic ecosystems, MOB (methane oxidising bacteria) are responsible for most of the methane oxidation, and they are also important in the surface layer of marine sediments. However, the fate of meth

  52. 水田土壌の物質循環と原生動物の機能

    村瀬 潤

    2006年度・日本微生物生態学会 学際シンポジュウム -土壌における原生生物の多様性と微生物との相互作用- 

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    Event date: 2006.11

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  53. 原生動物が水田土壌の物質循環に及ぼす影響

    村瀬 潤・Peter Frenzel・Matthias Noll

    日本土壌肥料学会2006年度秋田大会 

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    Event date: 2006.9

    Language:Japanese   Presentation type:Poster presentation  

    Country:Japan  

  54. 水田土壌の原生生物群集に及ぼすメタンの影響

    村瀬 潤・Peter Frenzel

    土壌微生物学会 

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    Event date: 2006

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  55. A microbial food web in a rice field soil driven by methane oxidation. International conference

    11th International Symposium on Microbial Ecology 

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    Event date: 2006

    Language:English   Presentation type:Oral presentation (general)  

  56. The impact of protists on activity and structure of bacterial community in a flooded rice field soil. International conference

    ASLO Summer Meeting 

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    Event date: 2005

    Language:English   Presentation type:Oral presentation (general)  

  57. Methane dynamics in Lake Biwa. International conference

    The Goldschmidt Conference 2003 

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    Event date: 2003

    Language:English  

  58. Carbon dynamics in bottom sediment of Lake Biwa, Japan: origins, metabolism, and impact on the lake ecosystem. International conference

    The International Symposium on Response of Terrestrial Watershed Ecosystems in Monsoon Asia to Global Change (Kyoto, Japan) 

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    Event date: 2002

    Language:English   Presentation type:Poster presentation  

    Country:Japan  

  59. Chemical determination of microbially reducible manganese and iron in paddy soils and lake sediments. International conference

    17th World Congress of Soil Science 

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    Event date: 2002

    Language:English   Presentation type:Poster presentation  

▼display all

Works 4

  1. 環境と微生物の事典編集委員

    2012.4
    -
    2014.8

  2. 陸水学事典

    2006

  3. 熱帯農業事典

    2003

  4. 琵琶湖堆積物の生元素の空間的分布  1, 52-55(1998)

    1998

Research Project for Joint Research, Competitive Funding, etc. 2

  1. Study on microbial interactions in a rice field soil for controlling methane emission

    2010.10 - 2011.9

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    Grant type:Competitive

  2. メタンが涵養する土壌微生物群集の解析

    2006

KAKENHI (Grants-in-Aid for Scientific Research) 19

  1. 水稲に連作障害が生じない本当の理由は何か:根圏で土壌病原菌を抑止する機作の探索

    Grant number:22K19132  2022.6 - 2025.3

    科学研究費助成事業  挑戦的研究(萌芽)

    浅川 晋, 村瀬 潤, 渡邉 健史

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    Authorship:Coinvestigator(s) 

    湛水下で栽培されるイネ(水稲)には土壌病原菌による連作障害が起きない。従来,湛水で生じる土壌の還元・嫌気状態により好気性の土壌病原菌の活動が抑制されることがその原因とされてきたが,イネと同様に湛水下で栽培されるレンコンやクワイにはフザリウム等の好気性糸状菌による連作障害が起きるため,他に原因があると思われる。イネの根圏では地上部より送られる酸素が根から漏出し酸化的となる。このようなイネの根圏で活発化する(1)原生生物の捕食作用,(2)根周囲の酸化還元境界層における二価鉄の酸化反応によるラジカル生成,(3)非病原性の細菌・糸状菌群集の増殖が土壌病原菌を抑止していると想定し,その機作を探る。

  2. Elucidation of dynamics of CO2 fixed by soil microbiome

    Grant number:22H05720  2022.6 - 2024.3

    Grants-in-Aid for Scientific Research  Grant-in-Aid for Transformative Research Areas (A)

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    Authorship:Principal investigator 

    Grant amount:\9490000 ( Direct Cost: \7300000 、 Indirect Cost:\2190000 )

  3. Fertilization effects on atmospheric methane uptake by a rubber tree plantation in Thailand: a direct microbial inhibition or an indirect stimulation by higher tree water use?

    Grant number:21KK0114  2021.10 - 2024.3

    科学研究費助成事業  国際共同研究加速基金(国際共同研究強化(B))

    EPRON Daniel, 東 若菜, 村瀬 潤

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    Authorship:Coinvestigator(s) 

    The project aims at characterising the effect of fertilisation on atmospheric methane by the soil, by testing two hypotheses: (1) fertilisation increases tree water use, reducing soil water content thus increasing the diffusion and the consumption of atmospheric; and (2) fertilization increases inorganic nitrogen and available phosphorus in the soil. Mineral nitrogen is known to inhibit methanotrophic activity when concentrations become high, while phosphorus may amplify or alleviate nitrogen inhibition.
    The proposal has been adopted in November 2021 and we have immediately started discussion through both on-line and face meeting for the implementation of the research project and to define precisely the protocols of the different activities that are planned for the next two years.
    We have started purchasing the equipment’s which will be used in continuously in Thailand during the project and prepared administrative paper works.
    The PI travelled to Thailand for one week on another budget, visited the field site and laboratory facilities and met with all Thai partners to prepare for the implementation of the research and agree on the degree of involvement of Thai partners and their responsibilities in the project.
    The project is progressing rather smoothly. All protocols have been detailed, discussed and shared with our Thai partners. The methane analyser (LI-7810, Licor, Lincoln, Nebraska USA) has been purchased and tested. Meetings have been organised with the Japanese team and with the Thai partner as schedule
    Due to Covid 19, the closure of the borders of Thailand which was only partially left in February and the border rules to return to Japan from a foreign country, it seemed reasonable to us to postpone the first campaign the first campaign of intensive measurements initially planned for March 2022 for a few months.
    Several operations have been conducted, including the plan and quotation for bringing electricity on site, sampling and sanding wood samples for anatomical studies, test of soil extraction protocols with the equipment’s available in Kasetsart University (Bangkok).
    We have also secured the procedures to export samples from Thailand and to import them to Japan, and the procedure for travelling with equipment. Administrative paper work is ongoing.
    We are fully ready to have our first intensive measurement campaign in early September
    The first intensive measurement campaign will be done in early September this year. A second one is planned in February 2023, a third one in August 2023 and the last one in March 2024.
    All planned activities will be conducted: long-term survey of soil methane uptake (from Sep 22 to March 24), the effect of the addition of fertilisers on the potential rates of methane oxidation (Aug 23), sap flow measurements to estimate tree water use (from Sep 22 to March 24), availability of the mineral forms of nitrogen and phosphorus (in Sep 22, Feb 23, Aug 23 and March 24), the estimation of gross mineralization and dissimilatory nitrate reduction to ammonium on intact soil cores in the field using the 15N pool dilution method (in Feb 23 and Aug 23) and the quantification of the abundances of the genes of the particulate CH4 monooxygenase methanotrophic bacteria and of the ammonia monooxygenase subunit A gene of the ammonia oxidising bacteria and archaea (in Feb 23 and Aug 23).
    Between each intensive measurement campaign, the Thai partner will be in charge of measuring soil methane flux bimonthly and to take care about the sap flow sensors

  4. 根圏マイクロバイオームを活用した持続型農業を実現するイネ遺伝子の探索と育種利用

    Grant number:21K18221  2021.7 - 2024.3

    科学研究費助成事業  挑戦的研究(開拓)

    中園 幹生, 村瀬 潤, 藤原 徹

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    Authorship:Coinvestigator(s) 

    低投入持続型農業を実現するには、少ない施肥でも収量を維持し安定した作物生産が可能となる品種の開発が有望視されており、そのためには少肥栽培時の作物の成長と収量の維持に寄与する遺伝子の単離とその育種利用が重要である。本研究では、イネを材料に用いて、無施肥条件下での成長・収量関連形質と根圏微生物との関係性を解明し、少肥栽培時の成長を支える根圏微生物との共生に関わるイネ遺伝子を同定することで、これらを遺伝子資源として育種利用することを目指す。
    低投入持続型農業を実現するには、少ない施肥でも収量を維持し安定した作物生産が可能となる品種の開発が有望視されており、そのためには少肥栽培時の作物の成長と収量の維持に寄与する遺伝子の単離とその育種利用が重要である。本研究では、イネを材料に用いて、無施肥条件下での成長・収量関連形質と根圏微生物との関係性を解明し、少肥栽培時の成長を支える根圏微生物との共生に関わるイネ遺伝子を同定することで、これらを遺伝子資源として育種利用することを目指す。
    令和3年度は、日本の栽培イネ120品種を施肥圃場と無施肥圃場で栽培し、草丈、分げつ数、SPAD値、乾物重、穂数、穂重、分げつ数、穂型の計測を行った。地上部形質のゲノムワイド関連解析(GWAS)により検出されたピークの中から、施肥区で2番染色体に、無施肥区で4番染色体に、施肥区及び無施肥区特異的にピークが検出された退化頴花率に着目した。GWASにより、この退化穎花率と有意に関連があるとされる複数の多型が検出され、これらの多型は穂型形成に関わる遺伝子近傍に存在していた。また、イネ120品種の根圏微生物叢データを用いて、主座標分析を行いイネ120品種の根圏微生物の群構造を示す主座標得点を用い、GWASを行った。その結果、1番染色体に無施肥区特異的なピークが検出され、候補責任遺伝子を絞り込んだ。この候補責任遺伝子の多型は、無施肥区の到穂日数、分げつ数においても影響を及ぼしていた。つまりこの候補責任遺伝子における多型が栄養条件に応答したイネの根系形成に関与し、その結果根圏微生物叢や地上部形質にも影響を及ぼしている可能性が考えられた。さらに、葉におけるイオノーム解析を進め、順調にデータが蓄積しており、令和4年度にGWASを実施予定である。
    当初の計画通りに進んでおり、日本の栽培イネ品種120系統の多様性に着目し、イネの地上部形質、ならびに根圏微生物叢との関連性について、イネのゲノム情報を用いてゲノムワイド関連解析(GWAS)を行った。その結果、退化頴花率などの地上部形質や根圏微生物の群構造決定に関わる候補責任遺伝子を同定できた。さらに、葉のイオノーム解析も順調に進捗しており、概ね、研究計画通りに本研究が進行している。
    今後(令和4年度)の研究の推進方策は以下の通りである。
    (1)無施肥圃場と施肥圃場で120系統を栽培して、栄養成長初期、開花期、登熟期、収穫期の4ステージで、栄養成長初期、開花期、登熟期では、草丈、分げつ数、SPAD値、地上部乾物重の4形質について、収穫期では、4形質に、穂数、穂重、籾数などの収量に関する7形質を加えた合計11形質について計測することで年次反復データを蓄積し、GWASを行う。
    (2)葉に蓄積している各元素をICP-MSによって網羅的に計測するイオノーム解析を実施し、NについてはCNコーダーを用いて計測する。これらの元素の蓄積パターンを、無施肥圃場と施肥圃場で栽培した120系統間で比較調査し、GWASを行う。
    (3)令和3年度に実施したGWASで同定できた候補責任遺伝子の機能を明らかにするために、CRISPR/Cas9によるノックアウト系統の作出を進める。

  5. Elucidation of the mechanism of waterlogging tolerance in genus Zea and its application to molecular breeding

    Grant number:21H04724  2021.4 - 2025.3

    Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (A)

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    Authorship:Coinvestigator(s) 

  6. 遺伝型・表現型が支配する水稲根圏の微生物食物連鎖構造と機能

    Grant number:20H02887  2020.4 - 2023.3

    科学研究費助成事業  基盤研究(B)

    村瀬 潤, 中園 幹生, 土井 一行, 西内 俊策

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    Authorship:Principal investigator 

    Grant amount:\17940000 ( Direct Cost: \13800000 、 Indirect Cost:\4140000 )

    根圏は植物と土壌微生物の相互作用のホットスポットである。植物根が土壌微生物に特徴的な生育環境を提供する一方、植物の良好で健全な生長は根圏微生物群集の構成やはたらきと深い関係にある。原生生物による捕食作用は、細菌・糸状菌の群集構造や活性に影響を与える重要なファクターであると認識されているが、根圏での原生生物のはたらきが他の微生物および植物の生育に与える影響はほとんど明らかにされていない。本研究では、水稲根圏における微生物食物連鎖の構造とその機能を解析し、イネの品種と根圏微生物との連関を明らかにする。
    前年度に引き続き、名古屋大学大学院生命農学研究科附属フィールド科学教育研究センター東郷フィールドの通常施肥水田および長期無施用水田においてジャポニカイネ120品種の栽培試験を行い、栽培1カ月後、出穂期、出穂2週間後、収穫期において地上部の形質調査を行った。前年度と本年度の地上部形質と120品種の遺伝形質との関係をGWASにて解析した結果、分げつ数や退化穎花率と複数の候補遺伝子との関連が示された。
    昨年度のイネ栽培試験における栽培1カ月後の根試料の原核微生物群集、真核微生物群集のアンプリコンシークエンスデータを全て取得した。原核微生物群集の主座標分析によって、すべての品種において施肥条件により根圏微生物叢が大きく変化することが明らかとなった。また、品種間でも根圏微生物叢に差が認めらた。主座標分析における主座標得点とイネ品種の遺伝型との関係をGWASにて解析し、微生物叢の違いと関連のある候補遺伝子が複数検出された。そのうちオーキシンの生合成に関わると考えられる遺伝子が低施肥条件における分げつ数の違いと関係していることが示された。
    3つの異なる分類群の水田土壌(グライ土、灰色低地土、黄色土)を用いて、施肥応答や到穂日数に基づく異なる表現型を示すイネ4品種を施肥および無施肥条件下でポット栽培した。土壌の違いによって施肥がイネの生育に及ぼす効果は異なっており、また、その効果はイネの品種によっても異なっていた。1カ月間の栽培の後、根圏微生物叢解析のために根試料を回収した。
    無施肥条件で重要な役割を果たすと想定される根圏窒素固定細菌群集のイネ品種や施肥条件による違いを明らかにするため、nifH遺伝子およびそのmRNAを対象とした分子生物学的解析手法の再検討を行った。
    イネ根圏微生物叢が施肥条件および品種によって異なることを明らかにすることができた。また、GWASによって、イネの遺伝形質と根圏微生物叢との関係や根圏微生物叢との関連を示すイネの遺伝子と地上部形質と関係を示唆する結果が得られたことから、本研究課題の主目的である、イネの表現型、遺伝型と根圏微生物叢との関係の一端を示すことができたと考えられる。一方で、今回は原核微生物(細菌・古細菌)叢との関係について解析を進めたが、真核微生物叢とイネの遺伝形質、表現形質との関係や、真核微生物叢と原核微生物叢との関係については今後解析を進める必要がある。また、栽培1カ月後(栄養成長期)のみならず出穂後(生殖成長期)の微生物叢についても同様の解析を進め、さらに根圏微生物叢について栽培年度の違いの有無を解析し、得られた結果の頑健性を検証する必要がある。
    窒素固定細菌群集の分子生物学的解析手法を再検討し、アンプリコンシークエンスや定量PCRの方法を定めることができた。今後窒素固定活性を測定した根試料の窒素固定細菌群集の構成やmRNAの発現を明らかにする予定である。
    初年度、2年目の栽培試験によって得られたイネ120品種の根試料の微生物叢の解析をさらに進め、生育時期(栄養成長期、生殖成長期)や栽培年度の違いとイネの遺伝形質、地上部形質との関係を明らかにする。また、根圏における原核微生物群集と真核微生物群集との関係を解析する。特に本研究課題のターゲットである捕食性原生生物群集と原核生物叢やイネの遺伝形質・表現形質との関係に注目して解析を進める。
    異なる土壌で栽培したイネの根圏微生物叢を解析し、イネの遺伝型・表現型とともに土壌型が根圏微生物に及ぼす影響を明らかにする。
    捕食性原生生物が根圏細菌・古細菌群集の形成やイネの生育に及ぼす影響について複数の土壌を用いた室内モデル実験で検証する。

  7. 田畑輪換圃場の微生物群集動態を長期継続調査し水田土壌微生物群集の安定性を解明する

    Grant number:18H02114  2018.4 - 2023.3

    科学研究費助成事業  基盤研究(B)

    浅川 晋, 村瀬 潤, 渡邉 健史

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    水田土壌中の微生物が極めて安定で頑健な群集を形成しているメカニズムの解明を目指し、田畑輪換圃場に注目し、田畑輪換による畑転換・水田復元に伴う各種土壌微生物群集の動態を長期間にわたり調査・解析する。田畑輪換の管理で生じる畑転換と水田復元が群集の存在量と構成に及ぼす影響や、群集の動態とその変化に影響を及ぼす要因を明らかにすることを目的とする。
    農研機構東北農業研究センター大仙研究拠点(秋田県大仙市)内の長期田畑輪換試験圃場の輪換区と対照の連年水田区より、ダイズあるいは水稲の播種・移植前(3月下旬)、水稲あるいはダイズの生育中(8月中旬)、収穫後(10月下旬)の3回作土層土壌を採取し、DNAおよびRNAの抽出用試料として調製・保存した。なお、輪換区については、ダイズ作後水稲作に戻され3年間の水田が続いた後に、2019年に再度ダイズ畑へと転換されており、2021年はダイズ作3年目であった。
    2008年から2019年(2010年は除く)の12年間にわたり採取した試料について16S rRNA遺伝子を対象にした定量PCR法により全細菌群集の存在量を調査した。輪換区はこの期間中2回の水田復元を挟み3 回畑転換が行われている。畑作あるいは水田期間による全細菌の存在量の変動には明確な傾向は見られなかった。輪換区の全細菌群集の存在量はオーダーとしては連年水田区とほぼ同じであったが、常に連年水田区よりも低い値を示し、水田復元期間に連年水田区と同程度まで増加するようなはっきりとした傾向は見られなかった。全細菌には好気性および嫌気性の菌群が含まれると考えられるが、2020年度に明らかにした鉄還元菌群集と同様に、畑転換の影響により全体として存在量が低下することが明らかになった。
    新型コロナウイルスの感染拡大のため、試料採取のための出張可能時期を見極める必要があったが、ダイズあるいは水稲の播種・移植前、水稲あるいはダイズの生育中と収穫後の3回について、対象とする田畑輪換試験圃場より継続的に土壌試料の採取を続けることができた。また、2008年から2019年にかけて12年間にわたる試料について、土壌中の物質代謝に大きな役割を果たしていると考えられる全細菌に対する畑転換の影響の特徴を明らかにすることができ、おおむね順調に研究が進展していると考えている。
    輪換区については2022年はダイズ畑転換3年目後に水田に復元される初年目となる予定であり、水田復元後の微生物群集の経時変化および土壌の酸化還元状態の進行を解析するための試料採取と調査を進めていく予定である。今後も感染拡大状況を勘案しながら試料採取のための出張可能時期を見極め、対象とする田畑輪換試験圃場より土壌試料の採取を継続し、得られた試料の解析を行っていく予定である。また、2022年度は最終年度となるため、得られたデータの取りまとめと結果の総括を行う予定としている。

  8. Development of integrated soil management technology towards rehabilitation of degraded soils in the Tropics

    Grant number:18H02315  2018.4 - 2022.3

    Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

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    Authorship:Coinvestigator(s) 

  9. Submillimeter-scale visualization of rhizosphere effect using Cryo-TOF-SIMS/SEM

    Grant number:16K15075  2016.4 - 2020.3

    Murase Jun

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    Authorship:Principal investigator 

    Grant amount:\3640000 ( Direct Cost: \2800000 、 Indirect Cost:\840000 )

    Chemical environments of rice rhizosphere were visualized at the submillimeter scale. Roots of rice seedlings grown in soft agar media were sampled together with the surrounding media without destruction of rhizosphere structure, and the distribution of dissolve compounds in the shock frozen root and rhizosphere was analyzed by using the Cryo-TOF-SIMS/SEM system. The results successfully demonstrated the chemical gradients in the rice rhizosphere within a several hundred micrometers. Environmental gradients in the rice rhizosphere were also demonstrated from the spatial distribution of protists. In addition, we showed that protozoan grazing plays a pivotal role in shaping the bacterial community in the rice rhizosphere.

  10. Effects of fluctuation of redox potentials on microbial dynamics and gas metabolisms of wetland soils

    Grant number:16H05056  2016.4 - 2019.3

    Murase Jun

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    Authorship:Principal investigator 

    Grant amount:\17680000 ( Direct Cost: \13600000 、 Indirect Cost:\4080000 )

    Focusing on paddy soil, we analyzed the fluctuation of the redox boundary layer and the response of the microbial community. In the paddy soil surface layer, the dissolved oxygen concentration showed extremely large fluctuation from supersaturation to complete anoxia. The oxidation-reduction potential also changed dramatically in the paddy rice rhizosphere according to the growth of rice. Protozoa living in paddy soils are considered to play an important role as a predator in the redox boundary layer, as there are groups with suitable oxygen concentration or redox potential and a group adaptable to a wide range of oxygen concentrations. Although methane oxidation bacteria require oxygen for methane oxidation, they were able to maintain their activity for a long time even under anaerobic conditions. Microaerobic iron-oxidizing bacteria act in the surface.

  11. Response of gas metabolism and microbial community in soil in the permafrost zone of northeastern Siberia to the environmental change

    Grant number:16H05618  2016.4 - 2019.3

    Murase Jun

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    Authorship:Principal investigator 

    Grant amount:\16250000 ( Direct Cost: \12500000 、 Indirect Cost:\3750000 )

    We investigated the actual conditions of the metabolism of the greenhouse gas methane in the northeastern Siberian permafrost wetland and the microbial communities involved in it and the response to environmental changes. While the methane oxidation potential, which is the key to methane emission, is found to be higher than that of the other arctic wetland soils, it has been shown that its function as a deterrent to methane emission is weak. Also, regarding the composition of the soil microbial community, the difference from the other arctic soils was clarified, and the uniqueness of this area was clarified.

  12. Elucidating the cause of strange odor production in Lake Ogawarako

    Grant number:15K07532  2015.4 - 2018.3

    Maie Nagamitsu

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    Authorship:Coinvestigator(s) 

    We unveiled physical environments of water in Lake Ogawarako, and influence of extreme weather events such as strong wind and typhoons to it. A 22-63% of total nitrogen and 38-79% of total phosphorous were distributed in the water layer from 15 to 21 m, which occupied only 13% of whole lake water. Their percentages significantly changed temporally, as such the layer is considered to be the major source of nitrogen and phosphorous in the upper layer. Lake Ogawarako was divided into 3 sub-region with different phytoplankton composition. Since iron concentration in lake water increased in autumn, when cyanobacteria became dominant, iron could be playing a role in the propagation of filamentous cyanobacteria. We isolated 15 strains of filamentous cyanobacteria, 5 strains of which had 2-MIB synthesis gene.

  13. 土壌生態系の解剖学的解析ツールとしての人工土壌の開発

    2013.4 - 2016.3

    科学研究費補助金 

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    Authorship:Principal investigator 

  14. 水稲根圏におけるメタンの動態に関わる微生物食物連鎖の構造と機能の解析

    2013.4 - 2016.3

    科学研究費補助金  基盤研究(B)

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    Authorship:Principal investigator 

  15. メタン酸化をめぐる微生物食物連鎖の機能解析                   

    2008

    科学研究費補助金  基盤研究(C),課題番号:20580061

    村瀬 潤

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    Authorship:Principal investigator 

  16. Diversity and function of protists in wetland soils

    2003.10 - 2008.3

    Grant-in-Aid for Scientific Research 

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    Authorship:Principal investigator 

  17. 微生物還元性鉄・マンガンの化学的定量法の確立と環境動態解析への応用

    2001.7 - 2003.1

    科学研究費補助金  奨励研究(A)

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    Authorship:Principal investigator 

    科研費

  18. 湖沼におけるガス交換

    1999.7 - 2002.1

    科学研究費補助金  特定領域研究(B)(2)(計画)

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    Authorship:Coinvestigator(s) 

    科研費

  19. 21世紀の環境構造変化に対応できる「琵琶湖標準モデル」の開発

    1998.7 - 2000.1

    科学研究費補助金  基盤研究(A)(2)(一般)

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    Authorship:Coinvestigator(s) 

    科研費

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Teaching Experience (On-campus) 4

  1. 微生物生態学

    2015

  2. 微生物の科学

    2015

  3. Introduction to Bioagricultural Science

    2011

  4. Environment and Human Interface

    2011

Teaching Experience (Off-campus) 5

  1. 環境微生物学

    2010.4 - 2011.3 中部大学)

  2. 2005.4 - 2006.3 名古屋女子大学)

  3. 環境微生物学

    2009.4 - 2010.3 中部大学)

  4. 2003.4 - 2004.3 名城大学)

  5. 2003.4 - 2004.3 名古屋女子大学)

 

Social Contribution 2

  1. 岡崎北高校連携講座

    2012.8

  2. 経済学研究科オープンカレッジ

    2010.4 - 2012.3