受賞区分：国内学会・会議・シンポジウム等の賞  受賞国：日本国

受賞国：日本国

受賞国：日本国

記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：nature  

Plants uptake nitrogen (N) from the soil mainly in the form of nitrate. However, nitrate is often distributed heterogeneously in natural soil. Plants, therefore, have a systemic long-distance signalling mechanism by which N starvation on one side of the root leads to a compensatory N uptake on the other N-rich side 1,2. This systemic N acquisition response is triggered by a root-to-shoot mobile peptide hormone, C-TERMINALLY ENCODED PEPTIDE (CEP), originating from the N-starved roots 3,4, but the molecular nature of the descending shoot-to-root signal remains elusive. Here, we show that phloem-specific polypeptides that are induced in leaves upon perception of root-derived CEP act as descending long-distance mobile signals translocated to each root. These shoot-derived polypeptides, which we named CEP DOWNSTREAM 1 (CEPD1) and CEPD2, upregulate the expression of the nitrate transporter gene NRT2.1 in roots specifically when nitrate is present in the rhizosphere. Arabidopsis plants deficient in this pathway show impaired systemic N acquisition response accompanied with N-deficiency symptoms. These fundamental mechanistic insights should provide a conceptual framework for understanding systemic nutrient acquisition responses in plants.

DOI： 10.1038/nplants.2017.29

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：American Association for the Advancement of Science  

Plants achieve mineral ion homeostasis by means of a hydrophobic barrier on endodermal cells called the Casparian strip, which restricts lateral diffusion of ions between the root vascular bundles and the soil. We identified a family of sulfated peptides required for contiguous Casparian strip formation in Arabidopsis roots. These peptide hormones, which we named Casparian strip integrity factor 1 (CIF1) and CIF2, are expressed in the root stele and specifically bind the endodermis-expressed leucine-rich repeat receptor kinase GASSHO1 (GSO1)/SCHENGEN3 and its homolog, GSO2. A mutant devoid of CIF peptides is defective in ion homeostasis in the xylem. CIF genes are environmentally responsive. Casparian strip regulation is not merely a passive process driven by root developmental cues; it also serves as an active strategy to cope with adverse soil conditions.

DOI： 10.1126/science.aai9057

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担当区分：最終著者,　責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1126/science.1257800

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担当区分：最終著者,　責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1126/science.1191132

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担当区分：最終著者,　責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1126/science.1150083

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担当区分：筆頭著者,　責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

記述言語：英語   出版者・発行元：New Phytologist  

Meristem activity is controlled by the CLAVATA (CLV) signaling pathway, which involves a suite of leucine-rich receptor (LRR) receptors, receptor-like proteins, and CLV-EMBRYO SURROUNDING REGION (CLE) peptide ligands. FASCIATED EAR 3 (FEA3) is a leucine-rich receptor (LRR) receptor-like protein important for meristem maintenance in maize and acts independently of canonical CLV receptors. To identify FEA3's interaction network, we used TurboID-based proximity labeling in Zea mays meristems and identified a putative co-receptor, BARELY ANY MERISTEM 1D (BAM1D). BAM1D and FEA3 proximity labeling proteomes shared over 40 proteins, including many signaling proteins, suggesting they feed into a common signaling pathway. fea3 was epistatic to bam1d in the control of inflorescence meristem (IM) size, supporting the idea that FEA3 and BAM1D interact physically. However, fea3 and bam1d act antagonistically because fea3 mutants had larger IMs, whereas bam1d mutants produced smaller IMs. This study demonstrates how in vivo TurboID-based proximity labeling clarifies complex genetic interactions between CLV receptors and expands our knowledge of downstream signaling components of CLV signaling pathways, which are largely uncharacterized. Our findings support the notion that multiple, partially overlapping CLV receptor complexes coordinately control meristem maintenance.

DOI： 10.1111/nph.70950

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Nature Communications  

In response to drought stress, land plants close their stomata to minimize transpiration. This action precedes a gradual accumulation of the stress hormone abscisic acid (ABA) that enhances plant drought tolerance. However, the molecular mechanisms that cause the time lag between the onset of stomatal closure and ABA accumulation and coordinate these two phases remain unexplained. Here, we found that Arabidopsis thaliana loss-of-function CLAVATA3/ENDOSPERM SURROUNDING REGION 5 (CLE5) mutants are less tolerant to drought. The CLE5 dodecapeptide (CLE5p) acts as a local signal to induce stomatal closure by binding to the LEUCINE-RICH REPEAT RECEPTOR-LIKE KINASE (LRR-RLK) receptor complex, BARELY ANY MERISTEM 1 (BAM1)–GUARD CELL HYDROGEN PEROXIDE-RESISTANT 1 (GHR1), in guard cells. The BAM1–GHR1–CLE5p module directly phosphorylates two SNF1-related protein kinases, OPEN STOMATA1 (SRK2E) and SRK2D, the central regulators of drought responses in plants, to regulate stomatal movement and drought-responsive gene expression without stimulating ABA biosynthesis or ROS accumulation. Our findings mark a critical step in understanding how plants promptly counteract environmental stresses. The CLEp–LRR-RLK signalling components are highly conserved across plant phyla, suggesting that peptide-mediated rapid stomatal closure is a widespread survival strategy and can be exploited to generate drought-resistant crops.

DOI： 10.1038/s41467-025-66392-6

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Bio Protocol  

Plant proteases participate in a wide variety of biological processes, including development, growth, and defense. To date, numerous proteases have been functionally identified through genetic studies. However, redundancy among certain proteases can obscure their roles, as single-gene loss-of-function mutants often exhibit no discernible phenotype, limiting identification through genetic approaches. Here, we describe an efficient system for the identification of target proteases that cleave specific substrates in the Arabidopsis apoplastic fluid. The method involves using Arabidopsis-submerged culture medium, which contains apoplastic proteases, followed by native two-dimensional electrophoresis. Gel fractionation and an in-gel peptide cleavage assay with a fluorescence-quenching peptide substrate are then used to detect specific proteolytic activity. The active fraction is then subjected to mass spectrometry–based proteomics to identify the protease of interest. This method allows for the efficient and comprehensive identification of proteases with specific substrate cleavage activities in the apoplast.

DOI： 10.21769/BioProtoc.5226

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Plant Journal  

In the accumulation response, chloroplasts move toward weak blue light (BL) to maximize photosynthetic efficiency; in the avoidance response, they move away from strong BL to reduce photodamage. The BL receptor kinase phototropin (phot) mediates these chloroplast relocation responses, and the chloroplast relocation response requires phot kinase activity. Upon receiving BL, phot undergoes autophosphorylation; however, the molecular mechanisms that regulate chloroplast relocation through phot autophosphorylation remain unclear. In this study, we conducted biochemical experiments using phot in the liverwort Marchantia polymorpha and revealed that phot employs cis-autophosphorylation under weak BL and both cis- and trans-autophosphorylation under strong BL. Inhibiting trans-autophosphorylation reduced phot autophosphorylation and suppressed the avoidance response, but not the accumulation response. These findings suggest that phot employs two modes of autophosphorylation to alternate between the accumulation and avoidance responses in plants.

DOI： 10.1111/tpj.17183

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Nature Research  

<jats:title>Abstract</jats:title><jats:p>Legumes control root nodule symbiosis (RNS) in response to environmental nitrogen availability. Despite the recent understanding of the molecular basis of external nitrate-mediated control of RNS, it remains mostly elusive how plants regulate physiological processes depending on internal nitrogen status. In addition, iron (Fe) acts as an essential element that enables symbiotic nitrogen fixation; however, the mechanism of Fe accumulation in nodules is poorly understood. Here, we focus on the transcriptome in response to internal nitrogen status during RNS in <jats:italic>Lotus japonicus</jats:italic> and identify that IRON MAN (IMA) peptide genes are expressed during symbiotic nitrogen fixation. We show that <jats:italic>LjIMA1</jats:italic> and <jats:italic>LjIMA2</jats:italic> expressed in the shoot and root play systemic and local roles in concentrating internal Fe to the nodule. Furthermore, IMA peptides have conserved roles in regulating nitrogen homeostasis by adjusting nitrogen-Fe balance in <jats:italic>L. japonicus</jats:italic> and <jats:italic>Arabidopsis thaliana</jats:italic>. These findings indicate that IMA-mediated Fe provision plays an essential role in regulating nitrogen-related physiological processes.</jats:p>

DOI： 10.1038/s41467-024-44865-4

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記述言語：英語   掲載種別：論文集(書籍)内論文   出版者・発行元：Methods in Molecular Biology  

Binding assays provide ultimate proof that a particular peptide and receptor kinase (RK) do indeed function as a ligand-receptor pair. Among available binding assays, proximity-induced photoaffinity labeling is superior for confirming direct contact between the peptide ligand and the receptor. Our binding assay employs covalent photoaffinity labeling followed by immunoprecipitation to specifically evaluate the ligand binding activity of the target RKs. Here, we describe a protocol for the synthesis of photoactivatable peptide ligands and the UV-induced formation of covalent bonds between photoaffinity ligands and RKs.

DOI： 10.1007/978-1-0716-3511-7_17

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Methods in Molecular Biology  

Various secreted peptides, including peptide hormones, are present in the apoplast, but their biochemical characterization remains a challenge due to their low abundance, difficulty in extraction, and interference from numerous secondary metabolites. Here, we describe a simple and straightforward protocol for the extraction of apoplastic peptides with a high purity. This protocol takes advantage of the fact that apoplastic peptides diffuse and accumulate in the culture medium when Arabidopsis seedlings are subjected to whole-plant submerged culture. The peptides in the culture medium are efficiently recovered by o-chlorophenol extraction followed by acetone precipitation. The recovered peptides can be subjected to nano-liquid chromatography coupled to tandem mass spectrometry (nano-LC-MS/MS) without any additional clean-up. This procedure enables the structural elucidation of mature peptide hormones in the apoplast with the use of Arabidopsis plants that overexpress peptide hormone genes.

DOI： 10.1007/978-1-0716-3511-7_6

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出版者・発行元：2024 Joint International Conference on Computational Linguistics Language Resources and Evaluation Lrec Coling 2024 Main Conference Proceedings  

Speakers sometimes omit certain arguments of a predicate in a sentence; such omission is especially frequent in pro-drop languages. This study addresses a question about ellipsis-what can explain the native speakers' ellipsis decisions?-motivated by the interest in human discourse processing and writing assistance for this choice. To this end, we first collect large-scale human annotations of whether and why a particular argument should be omitted across over 2,000 data points in the balanced corpus of Japanese, a prototypical pro-drop language. The data indicate that native speakers overall share common criteria for such judgments and further clarify their quantitative characteristics, e.g., the distribution of related linguistic factors in the balanced corpus. Furthermore, the performance of the language model-based argument ellipsis judgment model is examined, and the gap between the systems' prediction and human judgments in specific linguistic aspects is revealed. We hope our fundamental resource encourages further studies on natural human ellipsis judgment.

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Plant Journal  

Arabinogalactan proteins (AGPs) are a plant-specific family of extracellular proteoglycans characterized by large and complex galactose-rich polysaccharide chains. Functional elucidation of AGPs, however, has been hindered by the high degree of redundancy of AGP genes. To uncover as yet unexplored roles of AGPs in Arabidopsis, a mutant of Hyp O-galactosyltransferase (HPGT), a critical enzyme that catalyzes the common initial step of Hyp-linked arabinogalactan chain biosynthesis, was used. Here we show, using the hpgt1,2,3 triple mutant, that a reduction in functional AGPs leads to a stomatal patterning defect in which two or more stomata are clustered together. This defect is attributed to increased and dysregulated symplastic transport following changes in plasmodesmata structure, such that highly permeable complex branched plasmodesmata with cavities in branching parts increased in the mutant. We also found that the hpgt1,2,3 mutation causes a reduction of cellulose in the cell wall and accumulation of pectin, which controls cell wall porosity. Our results highlight the importance of AGPs in the correct biogenesis of plasmodesmata, possibly acting through the regulation of cell wall properties surrounding the plasmodesmata.

DOI： 10.1111/tpj.16061

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Communications Biology  

Ultrasmall algae have attracted the attention of biologists investigating the basic mechanisms underlying living systems. Their potential as effective organisms for producing useful substances is also of interest in bioindustry. Although genomic information is indispensable for elucidating metabolism and promoting molecular breeding, many ultrasmall algae remain genetically uncharacterized. Here, we present the nuclear genome sequence of an ultrasmall green alga of freshwater habitats, Medakamo hakoo. Evolutionary analyses suggest that this species belongs to a new genus within the class Trebouxiophyceae. Sequencing analyses revealed that its genome, comprising 15.8 Mbp and 7629 genes, is among the smallest known genomes in the Viridiplantae. Its genome has relatively few genes associated with genetic information processing, basal transcription factors, and RNA transport. Comparative analyses revealed that 1263 orthogroups were shared among 15 ultrasmall algae from distinct phylogenetic lineages. The shared gene sets will enable identification of genes essential for algal metabolism and cellular functions.

DOI： 10.1038/s42003-022-04367-9

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出版者・発行元：Rigakuryoho Kagaku  

[Purpose] This study clarified the relationship between sedentary behavior and dynapenia in community-dwelling elderly using ambulatory service implementation facilities. [Participants and Methods] The subjects were 161 users of two outpatient nursing homes who met the conditions. A cross-sectional design was adopted for this study. Determination of dynapenia was performed via grip strength (28 kg for men, and 18 kg for women) with reference to the definition of the Asian Working Group for Sarcopenia. [Results] Sedentary behavior showed an association with dynapenia even after controlling for age, sex, physical activity, cognitive function, and comorbidities. [Conclusion] Interventions focusing on sedentary behavior are important for preventing the development and exacerbation of geriatric syndromes in the elderly due to the progression of dynapenia.

DOI： 10.1589/RIKA.36.783

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出版者・発行元：Seikagaku  

DOI： 10.14952/SEIKAGAKU.2021.930400

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記述言語：日本語   掲載種別：研究論文（学術雑誌）   出版者・発行元：公益社団法人 有機合成化学協会  

In plants, there are several kinds of intercellular signal transduction systems, such as with phytohormones and with peptide hormones. Small post-translationally modified peptides, which act as cell-to-cell signaling molecules, constitute the largest group of peptide hormones with various functions in intercellular signal transductions. We explored peptide hormones by in silico gene screening coupled with biochemical peptide detection. We also identified specific direct ligand-binding receptors for these peptides. In general, receptor genes are highly redundant in Arabidopsis and thus it is often difficult to identify the specific receptors by using classical forward and reverse genetics. To overcome this problem, we identify peptide hormone receptors by detecting direct peptide hormone-receptor interaction using photoaffinity labeling with photoactivatable peptide derivatives and custom-made receptor library. This review summarizes how we discovered these peptide hormone-receptor pairs and how these pairs function in plant growth and development.

DOI： 10.5059/yukigoseikyokaishi.78.713

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記述言語：日本語  

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Communications Biology  

Intercellular signaling mediated by peptide hormones and membrane-localized receptor kinases plays crucial roles in plant developmental processes. Because of their diverse functions, agonistic or antagonistic modulation of peptide signaling holds enormous promise for agricultural applications. Here we established a high-throughput screening system using a bead-immobilized receptor kinase and fluorescent-labeled peptide ligand to identify small molecules that bind peptide hormone receptors in competition with natural ligands. We used the Arabidopsis CLE9-BAM1 ligand-receptor pair to screen a library of ≈30,000 chemicals and identified NPD12704 as an antagonist for BAM1. NPD12704 also inhibited CLV3 binding to BAM1 but only minimally interfered with CLV3 binding to CLV1, the closest homolog of BAM1, demonstrating preferential receptor specificity. Treatment of clv1-101 mutant seedlings with NPD12704 enhanced the enlarged shoot apical meristem phenotype. Our results provide a technological framework enabling high-throughput identification of small non-peptide chemicals that specifically control receptor kinase–mediated peptide hormone signaling in plants.

DOI： 10.1038/s42003-019-0307-8

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Oxford University Press  

Legumes can survive in nitrogen-deficient environments by forming root-nodule symbioses with rhizobial bacteria; however, forming nodules consumes energy, and nodule numbers must thus be strictly controlled. Previous studies identified major negative regulators of nodulation in Lotus japonicus, including the small peptides CLAVATA3/ESR (CLE)-RELATED-ROOT SIGNAL1 (CLE-RS1), CLE-RS2, and CLE-RS3, and their putative major receptor HYPERNODULATION AND ABERRANT ROOT FORMATION1 (HAR1). CLE-RS2 is known to be expressed in rhizobia-inoculated roots, and is predicted to be post-translationally arabinosylated, a modification essential for its activity. Moreover, all three CLE-RSs suppress nodulation in a HAR1-dependent manner. Here, we identified PLENTY as a gene responsible for the previously isolated hypernodulation mutant plenty. PLENTY encoded a hydroxyproline O-arabinosyltransferase orthologous to ROOT DETERMINED NODULATION1 in Medicago truncatula. PLENTY was localized to the Golgi, and an in vitro analysis of the recombinant protein demonstrated its arabinosylation activity, indicating that CLE-RS1/2/3 may be substrates for PLENTY. The constitutive expression experiments showed that CLE-RS3 was the major candidate substrate for PLENTY, suggesting the substrate preference of PLENTY for individual CLE-RS peptides. Furthermore, a genetic analysis of the plenty har1 double mutant indicated the existence of another PLENTY-dependent and HAR1-independent pathway negatively regulating nodulation.

DOI： 10.1093/jxb/ery364

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Developmental Cell  

In plants, the position of lateral roots (LRs) depends on initiation sites induced by auxin. The domain of high auxin response responsible for LR initiation stretches over several cells, but only a pair of pericycle cells (LR founder cells) will develop into LRs. In this work, we identified a signaling cascade controlling LR formation through lateral inhibition. It comprises a peptide hormone TARGET OF LBD SIXTEEN 2 (TOLS2), its receptor RLK7, and a downstream transcription factor PUCHI. TOLS2 is expressed at the LR founder cells and inhibits LR initiation. Time-lapse imaging of auxin-responsive DR5:LUCIFERASE reporter expression revealed that occasionally two pairs of LR founder cells are specified in close proximity even in wild-type and that one of them exists only transiently and disappears in an RLK7-dependent manner. We propose that the selection of LR founder cells by the peptide hormone-receptor cascade ensures proper LR spacing.

DOI： 10.1016/j.devcel.2018.11.031

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記述言語：日本語   出版者・発行元：東京 : 読売新聞東京本社調査研究本部 ; 2007-  

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Nature Plants  

Pectin is one of the three key cell wall polysaccharides in land plants and consists of three major structural domains: homogalacturonan, rhamnogalacturonan I (RG-I) and RG-II. Although the glycosyltransferase required for the synthesis of the homogalacturonan and RG-II backbone was identified a decade ago, those for the synthesis of the RG-I backbone, which consists of the repeating disaccharide unit [→2)-α-l-Rha-(1 → 4)-α-d-GalUA-(1→], have remained unknown. Here, we report the identification and characterization of Arabidopsis RG-I:rhamnosyltransferases (RRTs), which transfer the rhamnose residue from UDP-β-l-rhamnose to RG-I oligosaccharides. RRT1, which is one of the four Arabidopsis RRTs, is a single-spanning transmembrane protein, localized to the Golgi apparatus. RRT1 was highly expressed during formation of the seed coat mucilage, which is a specialized cell wall with abundant RG-I. Loss-of-function mutation in RRT1 caused a reduction in the level of RG-I in the seed coat mucilage. The RRTs belong to a novel glycosyltransferase family, now designated GT106. This is a large plant-specific family, and glycosyltransferases in this family seem to have plant-specific roles, such as biosynthesis of plant cell wall polysaccharides.

DOI： 10.1038/s41477-018-0217-7

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：日本学士院  

The identification of hormones and their receptors in multicellular organisms is one of the most exciting research areas and has lead to breakthroughs in understanding how their growth and development are regulated. In particular, peptide hormones offer advantages as cell-tocell signals in that they can be synthesized rapidly and have the greatest diversity in their structure and function. Peptides often undergo post-translational modifications and proteolytic processing to generate small oligopeptide hormones. In plants, such small post-translationally modified peptides constitute the largest group of peptide hormones. We initially explored this type of peptide hormone using bioassay-guided fractionation and later by in silico gene screening coupled with biochemical peptide detection, which led to the identification of four types of novel peptide hormones in plants. We also identified specific receptors for these peptides and transferases required for their posttranslational modification. This review summarizes how we discovered these peptide hormone- receptor pairs and post-translational modification enzymes, and how these molecules function in plant growth, development and environmental adaptation.

DOI： 10.2183/pjab.94.006

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Methods in Molecular Biology  

The formation of longitudinal zonation patterns is important for normal root development and is regulated by the transcription factor PLETHORA (PLT). PLT proteins form a concentration gradient, and PLT protein levels determine root zonation. A peptide hormone root meristem growth factor (RGF) and its receptors (RGFRs) act as key regulators of root development by regulating the PLT protein expression pattern. Here, we describe a method for monitoring PLT protein gradient patterns in Arabidopsis with exogenous RGF treatment.

DOI： 10.1007/978-1-4939-8772-6_9

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記述言語：日本語   出版者・発行元：一般社団法人 植物化学調節学会  

<p>During the past 20 years, biochemical, genetic, and bioinformatic analyses have identified more than a dozen secreted peptide hormones in plants.　These peptide hormones have proven to be functionally more diverse than anticipated.　The number of functionally characterized peptide hormones now exceeds the number of classical plant hormones and constitute one of the largest research areas in plant biology.　Because a number of uncharacterized genes encoding small secreted peptides have been identified in <i>Arabidopsis</i>, unraveling the signal transduction pathways mediated by secreted peptides is currently one of the most exciting targets.　This review series covers recent topics in peptide hormone research in plants.</p>

DOI： 10.18978/jscrp.53.2_91

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記述言語：日本語   出版者・発行元：一般社団法人 植物化学調節学会  

<p>Nitrogen （N） is a critical nutrient for plants but is often distributed unevenly in the soil.　Plants therefore have evolved a systemic mechanism by which N starvation on one side of the root system leads to a compensatory upregulation of nitrate uptake on the other side.　However, the molecular components underlying this long-distance signaling, called systemic N-demand signaling, have long remained elusive.　We identified that N starvation-induced small 15-amino-acid secreted peptide, CEP （C-terminally encoded peptide）, acts as a root-derived ascending N-demand signal to the shoot via xylem.　CEP is recognized in the shoot by specific LRR-receptor kinase, CEPR （CEP receptor）, that leads to the production of non-secreted polypeptide, CEPD （CEP downstream）, as a secondary signal.　CEPD acts as a shoot-derived descending signal to the root via phloem and ultimately upregulates nitrate transporter <i>NRT2.1</i> gene in the roots.　Thus, CEP family peptides induced on one side of the roots by local N starvation is able to mediate upregulation of <i>NRT2.1</i> in the distant part of the roots.　Our findings provide new insights into the molecular basis of plant adaptation to a dynamically fluctuating N environment.</p>

DOI： 10.18978/jscrp.53.2_93

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Nature Communications  

Evolution often diversifies a peptide hormone family into multiple subfamilies, which exert distinct activities by exclusive interaction with specific receptors. Here we show that systematic swapping of pre-existing variation in a subfamily of plant CLE peptide hormones leads to a synthetic bifunctional peptide that exerts activities beyond the original subfamily by interacting with multiple receptors. This approach provides new insights into the complexity and specificity of peptide signalling.

DOI： 10.1038/ncomms14318

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記述言語：英語   掲載種別：論文集(書籍)内論文   出版者・発行元：Methods in Molecular Biology  

Although more than 600 single-transmembrane receptor kinase genes have been found in the Arabidopsis genome, only a few of them have known physiological functions, and even fewer plant receptor kinases have known specific ligands. Ligand-binding analysis must be operated using the functionally expressed receptor form. However, the relative abundance of native receptor kinase molecules in the plasma membrane is often quite low. Here, we present a method for stable and functional expression of plant receptor kinases in tobacco BY-2 cells that allows preparation of microsomal fractions containing the receptor. This procedure provides a sufficient amount of receptor proteins while maintaining its ligand-binding activities.

DOI： 10.1007/978-1-4939-7063-6_3

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記述言語：英語   掲載種別：論文集(書籍)内論文   出版者・発行元：Methods in Molecular Biology  

Defining the ligand-binding activity of receptors is important because the binding of ligands is the initial reaction in secreted ligand-dependent cell-to-cell communication. Photoaffinity labeling is one of the most efficient biochemical techniques for detecting direct ligand-receptor interactions. Here, we describe photoaffinity labeling to visualize the direct interaction between peptide ligands and their receptors by using photoactivatable and radioactive peptide ligand derivatives.

DOI： 10.1007/978-1-4939-7063-6_6

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1016/j.pbi.2016.07.009

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1073/pnas.1604849113

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担当区分：最終著者,　責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1073/pnas.1522639113

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1111/tpj.13015

PubMed

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1093/jxb/erv105

Open Access

Web of Science

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1038/ng.3309

Web of Science

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1093/jxb/erv105

Open Access

PubMed

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1111/tpj.12817

Web of Science

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1111/tpj.12817

PubMed

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1111/tpj.12764

Web of Science

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1111/tpj.12764

PubMed

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1111/tpj.12475

Web of Science

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1146/annurev-arplant-050312-120122

Web of Science

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1146/annurev-arplant-050312-120122

PubMed

担当区分：最終著者,　責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1038/NCHEMBIO.1351

Web of Science

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1016/j.cub.2013.06.060

Web of Science

担当区分：責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1038/ncomms3191

Web of Science

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1093/pcp/pcs174

Open Access

Web of Science

担当区分：最終著者,　責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

Hydroxyproline (Hyp) O-arabinosylation is a posttranslational modification that is prominent in extracellular glycoproteins in plants. Hyp O-arabinosylation is generally found in these glycoproteins in the form of linear oligoarabinoside chains and plays a key role in their function by contributing to conformational stability. However, Hyp O-arabinosyltransferase (HPAT), a key enzyme that catalyzes the transfer of the L-arabinose to the hydroxyl group of Hyp residues, has remained undiscovered. Here, we purified and identified Arabidopsis HPAT as a Golgi-localized transmembrane protein that is structurally similar to the glycosyltransferase GT8 family. Loss-of-function mutations in HPAT genes cause pleiotropic phenotypes that include enhanced hypocotyl elongation, defects in cell wall thickening, early flowering, early senescence, and impaired pollen tube growth. Our results indicate essential roles of Hyp O-arabinosylation in both vegetative and reproductive growth in plants.

DOI： 10.1038/NCHEMBIO.1351

担当区分：筆頭著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

Arabinosylation of hydroxyproline (Hyp) is a posttranslational modification often found in secreted peptide signals in plants. The physiological importance of this modification was highlighted by the finding that CLAVATA3 (CLV3), a key peptide signal for regulating the fate of stem cells in shoot apical meristem (SAM) in Arabidopsis, contains three L-arabinose residues linked via linear β-1,2-linkages. However, understanding the functions and properties of arabinosylated peptides have been hindered by difficulties in synthesizing the complex arabinose chain. Here we report the stereoselective total synthesis of β-1,2-linked tri-arabinosylated CLV3 peptide ([Ara3]CLV3). Chemically synthesized [Ara3]CLV3 restricted stem cell activity more effectively than did unmodified CLV3 peptide. Comparison of mono-, di- and tri-arabinosylated CLV3 glycopeptides revealed that the biological activity increased progressively as arabinose chain length increased. Thus, arabinose chain length of CLV3 is important for its biological activity. NMR spectroscopy and NOE-based structure calculations further revealed the structural impact of the arabinose chain on peptide conformation. The arabinose chain of [Ara3]CLV3 extends toward the C-terminal end of the peptide, and its non-reducing end is positioned proximal to the peptide backbone. Consequently, the arabinose chain causes distinct distortion in the C-terminal half of the peptide in a highly directional manner. The established synthetic route of [Ara3]CLV3 will greatly contribute to our understanding of the biology and biochemistry of arabinosylated peptide signals in plants.

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1111/j.1365-313X.2012.04934.x

Web of Science

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1111/j.1365-2443.2011.01569.x

Web of Science

担当区分：筆頭著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

Leucine-rich repeat receptor kinases (LRR-RKs) comprise the largest subfamily of the transmembrane receptor kinases in plants. In several LRR-RKs, a loop-out region called an island domain that intercepts the extracellular tandem LRRs at a position near the transmembrane domain constitutes the ligand-binding pocket, but the absence of the island domain in numerous LRR-RKs raises questions about which domain specifically recognizes the corresponding ligands in non-island domain-carrying LRR-RKs. Here, we determined by photoaffinity labeling followed by chemical and enzymatic digestion that BAM1, a CLV1/BAM family LRR-RK whose extracellular domain is comprised of 22 consecutive LRRs, directly interacts with the small peptide ligand CLE9 at the LRR6-8 region that is relatively distal from the transmembrane domain. Multiple sequence alignment and homology modeling revealed that the inner concave side of LRR6-8 of the CLV1/BAM family LRR-RKs is slightly deviatory from the LRR consensus. Supporting our findings, the clv1-4 mutant carries a missense mutation at the inner concave side of LRR6 of CLV1, and introduction of the corresponding mutation into BAM1 resulted in complete loss of ligand-binding activity. Our results indicate that ligand recognition mechanisms of plant LRR-RKs are more complex and diversified than anticipated.

DOI： 10.1086/491575

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1093/pcp/pcq169

Open Access

Web of Science

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1016/j.pbi.2010.05.008

Web of Science

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1073/pnas.0902801106

Open Access

Web of Science

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1038/nchembio.182

Web of Science

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1104/pp.109.135954

Open Access

Web of Science

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1073/pnas.0808444105

Open Access

Web of Science

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1111/j.1365-313X.2008.03464.x

Web of Science

担当区分：筆頭著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

Peptidomics is a challenging field in which to create a link between genomic information and biological function through biochemical analysis of expressed peptides, including precise identification of post-translational modifications and proteolytic processing. We found that secreted peptides in Arabidopsis plants diffuse into the medium of whole-plant submerged cultures, and can be effectively identified by o-chlorophenol extraction followed by LC-MS analysis. Using this system, we first confirmed that a 12-amino-acid mature CLE44 peptide accumulated at a considerable level in the culture medium of transgenic plants overexpressing CLE44. Next, using an in silico approach, we identified a novel gene family encoding small secreted peptides that exhibit significant sequence similarity within the C-terminal short conserved domain. We determined that the mature peptide encoded by At1g47485, a member of this gene family, is a 15-amino-acid peptide containing two hydroxyproline residues derived from the conserved domain. This peptide, which we have named CEP1, is mainly expressed in the lateral root primordia and, when overexpressed or externally applied, significantly arrests root growth. CEP1 is a candidate for a novel peptide plant hormone.

DOI： 10.1111/j.1365-313X.2008.03464.x

担当区分：筆頭著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1073/pnas.0808444105

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1073/pnas.0706403104

Open Access

Web of Science

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1111/j.1365-313X.2007.03204.x

Web of Science

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1074/jbc.M604558200

Open Access

Web of Science

記述言語：英語   掲載種別：研究論文（学術雑誌）  

Phytosulfokine (PSK), an endogenous 5-amino-acid secreted peptide in plants, affects cellular potential for growth via binding to PSKR1, a member of the leucine-rich repeat receptor kinase (LRR-RK) family. PSK interacts with PSKR1 in a highly specific manner with a nanomolar dissociation constant. However, it is not known which residues in the PSKR1 extracellular domain constitute the ligand-binding pocket. Here, we identified the PSK-binding domain of carrot PSKR1 (DcPSKR1) by photoaffinity labeling. We cross-linked the photoactivatable PSK analog [125I]Ne-[(4-azidosalicyl)Lys5]PSK with DcPSKR1 using UV irradiation, and mapped the cross-linked region using chemical and enzymatic fragmentation. We also established a novel メon-column photoaffinity labelingモ methodology that allows repeated incorporation of the photoaffinity label to increase the efficiency of the photoaffinity cross-linking reactions. We purified a labeled DcPSKR1 tryptic fragment using anti-PSK antibodies, and identified a peptide fragment that corresponds to the 15-amino-acid Glu503-Lys517 region of DcPSKR1 by MALDI-TOF MS. Deletion of Glu503-Lys517 completely abolishes the ligand-binding activity of DcPSKR1. This region is in the island domain flanked by extracellular LRRs, indicating that this domain forms a ligand-binding pocket that directly interacts with PSK.

DOI： 10.1074/jbc.M604558200

Open Access

担当区分：筆頭著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

Despite the large number of receptor-like kinases (RLKs) in plants, few of them have known specific ligands. Ligand fishing is one of the most challenging post-genomic technologies. Here, we report a strategy for functional immobilization of plant RLKs on microbeads via covalent linkage. Due to the high density of immobilized RLK, ligand-receptor interaction can be visualized at high sensitivity using fluorescent-labeled ligands under the confocal laser scanning microscope. Moreover, using receptor-based affinity chromatography system with RLK microbeads, the ligand of the receptor was directly retrieved at high purity from complex natural samples. Our RLK microbeads and receptor-based ligand fishing approach is a feasible alternative to conventional forward genetics and bioassay-based biochemical purification for identification of novel ligand-receptor pairs in plants.

記述言語：英語   掲載種別：研究論文（学術雑誌）  

Web of Science

記述言語：英語   掲載種別：研究論文（学術雑誌）  

Web of Science

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1104/pp.106.081109

Web of Science

記述言語：英語   掲載種別：研究論文（学術雑誌）  

Phytosulfokine (PSK), a 5-amino-acid sulfated peptide that has been identified in conditioned medium of plant cell cultures, promotes cellular growth in vitro via binding to the membrane-localized PSK receptor. Here, we report that loss-of-function and gain-of-function mutations of the Arabidopsis PSK receptor (AtPSKR1) alter cellular longevity and potential for growth without interfering with basic morphogenesis of plants. Although mutant pskr1-1 plants exhibit morphologically normal growth until 3 weeks after germination, individual pskr1-1 cells gradually lose their potential to form calluses as tissues mature. Shortly after a pskr1-1 callus forms, it loses potential for growth, resulting in formation of a smaller callus than the wild-type. Leaves of pskr1-1 plants exhibit premature senescence after bolting. Leaves of AtPSKR1ox plants exhibit greater longevity and significantly greater potential for callus formation than leaves of wild-type plants, irrespective of their age. Calluses derived from AtPSKR1ox plants maintain their potential for growth longer than wild-type calluses. Combined with the present finding that PSK precursor genes are more strongly expressed in mature plant parts than in immature plant parts, the available evidence indicates that PSK signaling affects cellular longevity and potential for growth, and thereby exerts a pleiotropic effect on cultured tissue in response to environmental hormonal conditions.

DOI： 10.1104/pp.106.081109

担当区分：筆頭著者   記述言語：英語  

DOI： 10.1002/tcr.20090

担当区分：筆頭著者   記述言語：英語  

In recent years, numerous biochemical and genetic studies have demonstrated that peptide signaling plays a greater than anticipated role in various aspects of plant growth and development. A substantial proportion of these peptides are secretory and act as local signals mediating cell-to-cell communication. Specific receptors for several peptides were identified as being membrane-localized receptor kinases, the largest family of receptor-like molecules in plants. These findings illustrate the importance of peptide signaling in the regulation of plant growth, functions that were previously ascribed to the combined action of small lipophilic compounds referred to as メtraditional plant hormones.モ Here, we outline recent advances in the current understanding of biologically active peptides in plants, currently regarded as a new class of plant hormones.

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1146/annurev.arplant.56.032604.144204

Web of Science

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1002/tcr.20090

Web of Science

記述言語：英語   掲載種別：研究論文（学術雑誌）  

Web of Science

記述言語：英語   掲載種別：研究論文（学術雑誌）  

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1016/j.plantsci.2005.06.008

担当区分：筆頭著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

We have developed a novel procedure for concentrating sulfated peptides, as a front end to mass spectrometric analysis, based on ion-selective interaction of sulfate ions with anion exchangers. Ions with a higher charge and smaller solvated ion radius, such as sulfate ions, have higher retention in an ion exchanger, due to their greater degree of coulombic interactions. We tested the effectiveness of this approach for enrichment and identification of sulfated peptides, using a tryptic digest of bovine serum albumin spiked with model sulfated peptide (molar ratio, 20:1), and using a tryptic digest of bovine fibrinogen. Sulfated peptides are identified by mass spectrometry, in which both the molecular ion and its specific fragment ion produced by facile loss of SO3 are detected. In both experiments, sulfated peptides were strongly retained on the anion exchanger, and were eluted by higher concentrations of competing ion with minimum contamination of non-sulfated peptides. Using this procedure, we determined that the 13-amino-acid C-terminal peptide of the minor gユ chain of bovine fibrinogen contains sulfated tyrosine.

記述言語：英語   掲載種別：研究論文（学術雑誌）  

Web of Science

記述言語：英語   掲載種別：研究論文（学術雑誌）  

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1007/s00299-004-0816-9

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1093/pcp/pcg161

担当区分：筆頭著者   記述言語：英語  

DOI： 10.1242/jcs.00733

記述言語：日本語  

"Peptide Newsletter Japan, 41, 5-7"

記述言語：日本語  

DOI： 10.1271/nogeikagaku1924.75.1275

Open Access

記述言語：英語  

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1016/S1360-1385(01)02148-3

記述言語：英語   掲載種別：研究論文（学術雑誌）  

記述言語：英語   掲載種別：研究論文（学術雑誌）  

記述言語：英語   掲載種別：研究論文（学術雑誌）  

記述言語：英語   掲載種別：研究論文（学術雑誌）  

記述言語：英語  

記述言語：英語   掲載種別：研究論文（学術雑誌）  

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1088/0004-637X/749/2/173

記述言語：英語   掲載種別：研究論文（学術雑誌）  

記述言語：英語   掲載種別：研究論文（学術雑誌）  

記述言語：英語   掲載種別：研究論文（学術雑誌）  

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1007/s00441-003-0732-2

記述言語：英語   掲載種別：研究論文（学術雑誌）  

記述言語：英語   掲載種別：研究論文（学術雑誌）  

記述言語：英語  

DOI： 10.1007/s004250050518

記述言語：英語   掲載種別：研究論文（学術雑誌）  

記述言語：英語   掲載種別：研究論文（学術雑誌）  

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1023/A:1006058700949

記述言語：英語   掲載種別：研究論文（学術雑誌）  

記述言語：英語   掲載種別：研究論文（学術雑誌）  

記述言語：日本語  

記述言語：日本語  

DOI： 10.18978/jscrpi.32.2_160

Open Access

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1073/pnas.94.24.13357

記述言語：日本語  

DOI： 10.1271/nogeikagaku1924.70.588

Open Access

担当区分：筆頭著者,　責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

記述言語：英語   掲載種別：研究論文（学術雑誌）  

担当ページ：400-403   記述言語：日本語 著書種別：学術書

記述言語：日本語 著書種別：学術書

CiNii Research

CiNii Research

総ページ数：160  

CiNii Research

総ページ数：160  

CiNii Research

記述言語：日本語   出版者・発行元：一般社団法人 日本土壌肥料学会  

DOI： 10.20710/dohikouen.65.0_60_3

CiNii Research

researchmap

記述言語：日本語   出版者・発行元：一般社団法人 植物化学調節学会  

<p>To adapt ever-changing situations, multicellular organisms have developed several types of extracellular barriers as a result of evolutionary convergence.　Plant roots establish two types of extracellular barriers, namely Casparian strip （CS） and suberin lamellae, inside of the roots, not on their surface.　The Casparian strips mainly consist of lignin, a highly hydrophobic chemical, which blocks free diffusion of ions or small molecules across endodermal cell layers.　Despite of the physiological importance, the mechanisms underlying functional Casparian strip formation are not yet uncovered.　Recently, two independent groups identified exactly the same peptide-receptor pair for Casparian strip maturation by completely different approaches.　Geldner’s group in Lausanne identified the pair by forward genetics and database search, while Matsu-bayashi’s group in Nagoya identified the pair by <i>in silico</i> and biochemical screens.　This review focuses on how these two groups identified the sulfated peptides, CIF1/2 （<U>C</U>asparian strip <U>i</U>ntegrity <U>f</U>actors 1/2）, and how these peptides function on the Casparian strips.　Furthermore, we discuss the mode of action of the peptides during CS maturation in developmental context and their roles under physiological or stress conditions.</p>

DOI： 10.18978/jscrp.53.2_102

CiNii Research

J-GLOBAL

researchmap

J-GLOBAL

researchmap

開催年月日： 2023年11月

記述言語：英語   会議種別：口頭発表（基調）  

開催年月日： 2023年11月

記述言語：英語   会議種別：口頭発表（一般）  

開催年月日： 2023年10月

記述言語：英語   会議種別：口頭発表（基調）  

開催年月日： 2023年9月

記述言語：日本語   会議種別：ポスター発表  

開催地：札幌  

開催年月日： 2023年9月

記述言語：日本語   会議種別：ポスター発表  

開催地：札幌  

開催年月日： 2023年7月

記述言語：英語   会議種別：口頭発表（招待・特別）  

開催地：Gyeongju, Korea  

開催年月日： 2023年6月

記述言語：英語   会議種別：口頭発表（基調）  

開催年月日： 2023年6月

記述言語：英語   会議種別：ポスター発表  

開催年月日： 2023年6月

記述言語：英語   会議種別：ポスター発表  

開催年月日： 2023年3月

記述言語：日本語   会議種別：シンポジウム・ワークショップ パネル（指名）  

開催地：京都  

開催年月日： 2023年2月

記述言語：英語   会議種別：シンポジウム・ワークショップ パネル（指名）  

開催地：岡山   国名：日本国  

開催年月日： 2022年11月

記述言語：日本語   会議種別：シンポジウム・ワークショップ パネル（指名）  

開催地：名古屋  

開催年月日： 2022年11月

記述言語：日本語   会議種別：シンポジウム・ワークショップ パネル（指名）  

開催地：名古屋  

開催年月日： 2022年9月

記述言語：英語   会議種別：シンポジウム・ワークショップ パネル（指名）  

開催地：済州島   国名：大韓民国  

開催年月日： 2022年9月

記述言語：日本語   会議種別：シンポジウム・ワークショップ パネル（指名）  

開催地：京都  

開催年月日： 2021年12月

記述言語：日本語   会議種別：シンポジウム・ワークショップ パネル（指名）  

開催地：横浜  

開催年月日： 2021年10月

記述言語：日本語   会議種別：シンポジウム・ワークショップ パネル（指名）  

開催年月日： 2007年7月

記述言語：英語   会議種別：口頭発表（招待・特別）  

開催年月日： 2007年6月

記述言語：英語   会議種別：口頭発表（招待・特別）  

開催年月日： 2005年10月

記述言語：英語   会議種別：口頭発表（招待・特別）  

国名：日本国