Updated on 2024/03/26

写真a

 
MATSUBAYASHI Yoshikatsu
 
Organization
Graduate School of Science Professor
Graduate School
Graduate School of Science
Undergraduate School
School of Science Department of Biological Science
Title
Professor
Contact information
メールアドレス
External link

Degree 1

  1. 博士(農学) ( 1997.10   名古屋大学 ) 

Research Interests 6

  1. ペプチドホルモン

  2. リガンド

  3. 翻訳後修飾

  4. 分泌型ペプチド

  5. 受容体

  6. 細胞間情報伝達

Research Areas 2

  1. Others / Others  / Plant Physiology

  2. Others / Others  / Biological Production Chemistry/Biological Organic Chemistry

Current Research Project and SDGs 1

  1. 細胞間シグナルと受容体の解析から探る植物のかたちづくりと環境応答

Research History 5

  1. Nagoya University   Professor

    2014.4

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    Country:Japan

  2. 自然科学研究機構 基礎生物学研究所   教授

    2011.1 - 2014.3

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    Country:Japan

  3. Nagoya University   Associate professor

    2007.4 - 2010.12

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    Country:Japan

  4. Nagoya University   Associate Professor

    2002.4 - 2007.3

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    Country:Japan

  5. Nagoya University   Assistant

    1999.1 - 2002.3

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    Country:Japan

Education 2

  1. Nagoya University

    - 1997.10

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    Country: Japan

  2. Nagoya University   Faculty of Agriculture

    - 1993.3

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    Country: Japan

Professional Memberships 5

  1. 日本植物生理学会

  2. 日本分子生物学会

  3. 植物化学調節学会

  4. 日本植物学会

  5. 日本農芸化学会

Awards 7

  1. IPGSA Silver Medal

    2019.6   International Plant Growth Substances Association  

    松林嘉克

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    Award type:Award from international society, conference, symposium, etc.  Country:Japan

  2. 読売テクノフォーラム・ゴールドメダル賞

    2019.5   読売テクノフォーラム  

    松林嘉克

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    Award type:Award from publisher, newspaper, foundation, etc.  Country:Japan

  3. 井上学術賞

    2019.2   井上科学振興財団  

    松林嘉克

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    Award type:Award from publisher, newspaper, foundation, etc.  Country:Japan

  4. 日本学術振興会賞

    2016.2   日本学術振興会  

    松林嘉克

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    Award type:Award from publisher, newspaper, foundation, etc.  Country:Japan

  5. 日本分子生物学会三菱化学奨励賞

    2010.12   日本分子生物学会  

    松林嘉克

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    Award type:Award from Japanese society, conference, symposium, etc.  Country:Japan

  6. 日本植物生理学会奨励賞

    2008.3   日本植物生理学会  

    松林嘉克

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    Country:Japan

  7. 農芸化学奨励賞

    2001.3   日本農芸化学会  

    松林嘉克

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    Country:Japan

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Papers 107

  1. Peptide ligand-mediated trade-off between plant growth and stress response Reviewed International journal

    Ogawa-Ohnishi M., Yamashita T., Kakita M., Nakayama T., Ohkubo Y., Hayashi Y., Yamashita Y., Nomura T., Noda S., Shinohara H., Matsubayashi Y.

    SCIENCE   Vol. 378 ( 6616 ) page: 175 - 180   2022.10

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    Authorship:Last author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1126/science.abq5735

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  2. A peptide hormone required for Casparian strip diffusion barrier formation in Arabidopsis roots. Reviewed International journal

    Nakayama T, Shinohara H, Tanaka M, Baba K, Ogawa-Ohnishi M, Matsubayashi Y

    SCIENCE   Vol. 355 ( 6322 ) page: 284 - 286   2017.1

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    Authorship:Last author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1126/science.aai9057

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  3. Perception of root-derived peptides by shoot LRR-RKs mediates systemic N-demand signaling Reviewed

    Tabata Ryo, Sumida Kumiko, Yoshii Tomoaki, Ohyama Kentaro, Shinohara Hidefumi, Matsubayashi Yoshikatsu

    SCIENCE   Vol. 346 ( 6207 ) page: 343-346   2014.10

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    Authorship:Last author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1126/science.1257800

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  4. Secreted Peptide Signals Required for Maintenance of Root Stem Cell Niche in Arabidopsis Reviewed

    Matsuzaki Yo, Ogawa-Ohnishi Mari, Mori Ayaka, Matsubayashi Yoshikatsu

    SCIENCE   Vol. 329 ( 5995 ) page: 1065-1067   2010.8

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    Authorship:Last author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1126/science.1191132

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  5. Arabidopsis CLV3 peptide directly binds CLV1 ectodomain Reviewed

    Ogawa Mari, Shinohara Hidefumi, Sakagami Youji, Matsubayashi Yoshikatsu

    SCIENCE   Vol. 319 ( 5861 ) page: 294-294   2008.1

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    Authorship:Last author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1126/science.1150083

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  6. An LRR receptor kinase involved in the perception of a plant peptide hormone, phytosulfokine Reviewed

    Matsubayashi Y., Ogawa M., Morita A. and Sakagami Y.

    SCIENCE   Vol. 296   page: 1470-1472   2002.5

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    Authorship:Lead author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

  7. IMA peptides regulate root nodulation and nitrogen homeostasis by providing iron according to internal nitrogen status Reviewed

    Ito, M; Tajima, Y; Ogawa-Ohnishi, M; Nishida, H; Nosaki, S; Noda, M; Sotta, N; Kawade, K; Kamiya, T; Fujiwara, T; Matsubayashi, Y; Suzaki, T

    NATURE COMMUNICATIONS   Vol. 15 ( 1 )   2024.1

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  8. Arabinogalactan protein polysaccharide chains are required for normal biogenesis of plasmodesmata Reviewed

    Okawa, R; Hayashi, Y; Yamashita, Y; Matsubayashi, Y; Ogawa-Ohnishi, M

    PLANT JOURNAL   Vol. 113 ( 3 ) page: 493 - 503   2023.2

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    DOI: 10.1111/tpj.16061

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  9. Genomic analysis of an ultrasmall freshwater green alga, <i>Medakamo hakoo</i> Reviewed

    Kato, S; Misumi, O; Maruyama, S; Nozaki, H; Tsujimoto-Inui, Y; Takusagawa, M; Suzuki, S; Kuwata, K; Noda, S; Ito, N; Okabe, Y; Sakamoto, T; Yagisawa, F; Matsunaga, TM; Matsubayashi, Y; Yamaguchi, H; Kawachi, M; Kuroiwa, H; Kuroiwa, T; Matsunaga, S

    COMMUNICATIONS BIOLOGY   Vol. 6 ( 1 )   2023.1

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1038/s42003-022-04367-9

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  10. A type 2C protein phosphatase activates high-affinity nitrate uptake by dephosphorylating NRT2.1 Reviewed International journal

    NATURE PLANTS   Vol. 7 ( 3 ) page: 310 - +   2021.3

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1038/s41477-021-00870-9

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  11. Identification of Receptors of Plant Peptide Hormones by Photoaffinity Labeling

    Shinohara, H; Matsubayashi, Y

    JOURNAL OF SYNTHETIC ORGANIC CHEMISTRY JAPAN   Vol. 78 ( 7 ) page: 713 - 722   2020.7

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    Language:Japanese  

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  12. Shoot-to-root mobile CEPD-like 2 integrates shoot nitrogen status to systemically regulate nitrate uptake in Arabidopsis Reviewed International journal

    NATURE COMMUNICATIONS   Vol. 11 ( 1 )   2020.1

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1038/s41467-020-14440-8

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  13. Screening and identification of a non-peptide antagonist for the peptide hormone receptor in <i>Arabidopsis</i>

    Shinohara, H; Yasue, N; Onuki, T; Kondoh, Y; Yoshida, M; Matsubayashi, Y

    COMMUNICATIONS BIOLOGY   Vol. 2   2019.2

  14. PLENTY, a hydroxyproline <i>O</i>-arabinosyltransferase, negatively regulates root nodule symbiosis in <i>Lotus japonicus</i>

    Yoro, E; Nishida, H; Ogawa-Ohnishi, M; Yoshida, C; Suzaki, T; Matsubayashi, Y; Kawaguchi, M

    JOURNAL OF EXPERIMENTAL BOTANY   Vol. 70 ( 2 ) page: 507 - 517   2019.1

  15. Lateral Inhibition by a Peptide Hormone-Receptor Cascade during Arabidopsis Lateral Root Founder Cell Formation

    Toyokura Koichi, Goh Tatsuaki, Shinohara Hidefumi, Shinoda Akinori, Kondo Yuki, Okamoto Yoshie, Uehara Takeo, Fujimoto Koichi, Okushima Yoko, Ikeyama Yoshifumi, Nakajima Keiji, Mimura Tetsuro, Tasaka Masao, Matsubayashi Yoshikatsu, Fukaki Hidehiro

    DEVELOPMENTAL CELL   Vol. 48 ( 1 ) page: 64 - +   2019.1

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.devcel.2018.11.031

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  16. Pectin RG-I rhamnosyltransferases represent a novel plant-specific glycosyltransferase family

    Takenaka Yuto, Kato Kohei, Ogawa-Ohnishi Mari, Tsuruhama Kana, Kajiura Hiroyuki, Yagyu Kenta, Takeda Atsushi, Takeda Yoichi, Kunieda Tadashi, Hara-Nishimura Ikuko, Kuroha Takeshi, Nishitani Kazuhiko, Matsubayashi Yoshikatsu, Ishimizu Takeshi

    NATURE PLANTS   Vol. 4 ( 9 ) page: 669 - 676   2018.9

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1038/s41477-018-0217-7

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  17. Exploring peptide hormones in plants: identification of four peptide hormone-receptor pairs and two post-translational modification enzymes

    Matsubayashi Yoshikatsu

    PROCEEDINGS OF THE JAPAN ACADEMY SERIES B-PHYSICAL AND BIOLOGICAL SCIENCES   Vol. 94 ( 2 ) page: 59 - 74   2018.2

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.2183/pjab.94.006

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  18. Analysis of PLETHORA Gradient Formation by Secreted Peptides During Root Development

    Shinohara, H; Matsubayashi, Y

    MORPHOGEN GRADIENTS: METHODS AND PROTOCOLS   Vol. 1863   page: 155 - 164   2018

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  19. Shoot-to-root mobile polypeptides involved in systemic regulation of nitrogen acquisition Reviewed International journal

    Ohkubo Y, Tanaka M, Tabata R, Ogawa-Ohnishi M, Matsubayashi Y

    NATURE PLANTS   Vol. 3 ( 4 ) page: 17029   2017.4

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1038/nplants.2017.29

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  20. Cryptic bioactivity capacitated by synthetic hybrid plant peptides. Reviewed International journal

    Hirakawa Y, Shinohara H, Welke K, Irle S, Matsubayashi Y, Torii KU, Uchida N

    NATURE COMMUNICATIONS   Vol. 8   page: 14318   2017.2

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1038/ncomms14318

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  21. Photoaffinity Labeling of Plant Receptor Kinases. Reviewed

    Shinohara H, Matsubayashi Y

    Methods in molecular biology (Clifton, N.J.)   Vol. 1621   page: 59 - 68   2017

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    Language:Japanese   Publishing type:Research paper (scientific journal)  

    DOI: 10.1007/978-1-4939-7063-6_6

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  22. Expression of Plant Receptor Kinases in Tobacco BY-2 Cells. Reviewed

    Shinohara H, Matsubayashi Y

    Methods in molecular biology (Clifton, N.J.)   Vol. 1621   page: 29 - 35   2017

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    Language:Japanese   Publishing type:Research paper (scientific journal)  

    DOI: 10.1007/978-1-4939-7063-6_3

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  23. Long-distance peptide signaling essential for nutrient homeostasis in plants.

    Okamoto S, Tabata R, Matsubayashi Y

    Current opinion in plant biology   Vol. 34   page: 35-40   2016.12

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    DOI: 10.1016/j.pbi.2016.07.009

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  24. Loss of function at RAE2, a previously unidentified EPFL, is required for awnlessness in cultivated Asian rice.

    Bessho-Uehara K, Wang DR, Furuta T, Minami A, Nagai K, Gamuyao R, Asano K, Angeles-Shim RB, Shimizu Y, Ayano M, Komeda N, Doi K, Miura K, Toda Y, Kinoshita T, Okuda S, Higashiyama T, Nomoto M, Tada Y, Shinohara H, Matsubayashi Y, Greenberg A, Wu J, Yasui H, Yoshimura A, Mori H, McCouch SR, Ashikari M

    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA   Vol. 113 ( 32 ) page: 8969-74   2016.8

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1073/pnas.1604849113

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  25. Identification of three LRR-RKs involved in perception of root meristem growth factor in Arabidopsis. Reviewed

    Shinohara H, Mori A, Yasue N, Sumida K, Matsubayashi Y

    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA   Vol. 113 ( 14 ) page: 3897-902   2016.4

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    DOI: 10.1073/pnas.1522639113

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  26. A comprehensive strategy for identifying long-distance mobile peptides in xylem sap. Reviewed

    Okamoto S, Suzuki T, Kawaguchi M, Higashiyama T, Matsubayashi Y

    The Plant journal : for cell and molecular biology   Vol. 84 ( 3 ) page: 611-20   2015.11

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    DOI: 10.1111/tpj.13015

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  27. An Arabidopsis thaliana copper-sensitive mutant suggests a role of phytosulfokine in ethylene production Reviewed

    Wu Tao, Kamiya Takehiro, Yumoto Hiroko, Sotta Naoyuki, Katsushi Yamaguchi, Shigenobu Shuji, Matsubayashi Yoshikatsu, Fujiwara Toru

    JOURNAL OF EXPERIMENTAL BOTANY   Vol. 66 ( 13 ) page: 3657-3667   2015.7

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    DOI: 10.1093/jxb/erv105

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  28. A cascade of arabinosyltransferases controls shoot meristem size in tomato Reviewed

    Xu Cao, Liberatore Katie L., MacAlister Cora A., Huang Zejun, Chu Yi-Hsuan, Jiang Ke, Brooks Christopher, Ogawa-Ohnishi Mari, Xiong Guangyan, Pauly Markus, Van Eck Joyce, Matsubayashi Yoshikatsu, van der Knaap Esther, Lippman Zachary B.

    NATURE GENETICS   Vol. 47 ( 7 ) page: 784-+   2015.7

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1038/ng.3309

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  29. An Arabidopsis thaliana copper-sensitive mutant suggests a role of phytosulfokine in ethylene production. Reviewed

    Wu T, Kamiya T, Yumoto H, Sotta N, Katsushi Y, Shigenobu S, Matsubayashi Y, Fujiwara T

    Journal of experimental botany   Vol. 66 ( 13 ) page: 3657-67   2015.7

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    DOI: 10.1093/jxb/erv105

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  30. Reevaluation of the CLV3-receptor interaction in the shoot apical meristem: dissection of the CLV3 signaling pathway from a direct ligand-binding point of view Reviewed

    Shinohara Hidefumi, Matsubayashi Yoshikatsu

    PLANT JOURNAL   Vol. 82 ( 2 ) page: 328-336   2015.4

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1111/tpj.12817

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  31. Reevaluation of the CLV3-receptor interaction in the shoot apical meristem: dissection of the CLV3 signaling pathway from a direct ligand-binding point of view. Reviewed

    Shinohara H, Matsubayashi Y

    The Plant journal : for cell and molecular biology   Vol. 82 ( 2 ) page: 328-36   2015.4

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1111/tpj.12817

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  32. Identification of three potent hydroxyproline O-galactosyltransferases in Arabidopsis Reviewed

    Ogawa-Ohnishi Mari, Matsubayashi Yoshikatsu

    PLANT JOURNAL   Vol. 81 ( 5 ) page: 736-746   2015.3

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1111/tpj.12764

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  33. Identification of three potent hydroxyproline O-galactosyltransferases in Arabidopsis. Reviewed

    Ogawa-Ohnishi M, Matsubayashi Y

    The Plant journal : for cell and molecular biology   Vol. 81 ( 5 ) page: 736-46   2015.3

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1111/tpj.12764

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  34. CLE6 expression recovers gibberellin deficiency to promote shoot growth in Arabidopsis Reviewed

    Bidadi Haniyeh, Matsuoka Keita, Sage-Ono Kimiyo, Fukushima Jun, Pitaksaringkarn Weerasak, Asahina Masashi, Yamaguchi Shinjiro, Sawa Shinichiro, Fukuda Hiroo, Matsubayashi Yoshikatsu, Ono Michiyuki, Satoh Shinobu

    PLANT JOURNAL   Vol. 78 ( 2 ) page: 241-252   2014.4

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1111/tpj.12475

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  35. Posttranslationally Modified Small-Peptide Signals in Plants Reviewed

    Matsubayashi Yoshikatsu

    ANNUAL REVIEW OF PLANT BIOLOGY, VOL 65   Vol. 65   page: 385-413   2014

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1146/annurev-arplant-050312-120122

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  36. Posttranslationally modified small-peptide signals in plants. Reviewed

    Matsubayashi Y

    Annual review of plant biology   Vol. 65   page: 385-413   2014

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    DOI: 10.1146/annurev-arplant-050312-120122

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  37. Identification of three hydroxyproline O-arabinosyltransferases in Arabidopsis thaliana Reviewed

    Ogawa-Ohnishi Mari, Matsushita Wataru, Matsubayashi Yoshikatsu

    NATURE CHEMICAL BIOLOGY   Vol. 9 ( 11 ) page: 726-+   2013.11

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    Authorship:Last author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1038/NCHEMBIO.1351

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  38. A Novel Pollen-Pistil Interaction Conferring High-Temperature Tolerance during Reproduction via CLE45 Signaling Reviewed

    Endo Satoshi MS, Shinohara Hidefumi, Matsubayashi Yoshikatsu, Fukuda Hiroo

    CURRENT BIOLOGY   Vol. 23 ( 17 ) page: 1670-1676   2013.9

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.cub.2013.06.060

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  39. Root-derived CLE glycopeptides control nodulation by direct binding to HAR1 receptor kinase Reviewed

    Okamoto Satoru, Shinohara Hidefumi, Mori Tomoko, Matsubayashi Yoshikatsu, Kawaguchi Masayoshi

    NATURE COMMUNICATIONS   Vol. 4   2013.8

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    Authorship:Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1038/ncomms3191

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  40. Chemical Synthesis of Arabidopsis CLV3 Glycopeptide Reveals the Impact of Hydroxyproline Arabinosylation on Peptide Conformation and Activity Reviewed

    Shinohara Hidefumi, Matsubayashi Yoshikatsu

    PLANT AND CELL PHYSIOLOGY   Vol. 54 ( 3 ) page: 369-374   2013.3

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1093/pcp/pcs174

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  41. Identification of three hydroxyproline O-arabinosyltransferases in Arabidopsis thaliana Reviewed

    Ogawa-Ohnishi M., Matsushita W., Matsubayashi Y.

    NATURE CHEMICAL BIOLOGY   Vol. 9   page: 726-730   2013.1

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    Hydroxyproline (Hyp) O-arabinosylation is a posttranslational modification that is prominent in extracellular glycoproteins in plants. Hyp O-arabinosylation is generally found in these glycoproteins in the form of linear oligoarabinoside chains and plays a key role in their function by contributing to conformational stability. However, Hyp O-arabinosyltransferase (HPAT), a key enzyme that catalyzes the transfer of the L-arabinose to the hydroxyl group of Hyp residues, has remained undiscovered. Here, we purified and identified Arabidopsis HPAT as a Golgi-localized transmembrane protein that is structurally similar to the glycosyltransferase GT8 family. Loss-of-function mutations in HPAT genes cause pleiotropic phenotypes that include enhanced hypocotyl elongation, defects in cell wall thickening, early flowering, early senescence, and impaired pollen tube growth. Our results indicate essential roles of Hyp O-arabinosylation in both vegetative and reproductive growth in plants.

  42. Chemical synthesis of Arabidopsis CLV3 glycopeptide reveals the impact of hydroxyproline arabinosylation on peptide conformation and activity. Reviewed

    Shinohara H. and Matsubayashi Y.

    Plant Cell Physiol.   Vol. 54   page: 369-374   2013

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    Arabinosylation of hydroxyproline (Hyp) is a posttranslational modification often found in secreted peptide signals in plants. The physiological importance of this modification was highlighted by the finding that CLAVATA3 (CLV3), a key peptide signal for regulating the fate of stem cells in shoot apical meristem (SAM) in Arabidopsis, contains three L-arabinose residues linked via linear β-1,2-linkages. However, understanding the functions and properties of arabinosylated peptides have been hindered by difficulties in synthesizing the complex arabinose chain. Here we report the stereoselective total synthesis of β-1,2-linked tri-arabinosylated CLV3 peptide ([Ara3]CLV3). Chemically synthesized [Ara3]CLV3 restricted stem cell activity more effectively than did unmodified CLV3 peptide. Comparison of mono-, di- and tri-arabinosylated CLV3 glycopeptides revealed that the biological activity increased progressively as arabinose chain length increased. Thus, arabinose chain length of CLV3 is important for its biological activity. NMR spectroscopy and NOE-based structure calculations further revealed the structural impact of the arabinose chain on peptide conformation. The arabinose chain of [Ara3]CLV3 extends toward the C-terminal end of the peptide, and its non-reducing end is positioned proximal to the peptide backbone. Consequently, the arabinose chain causes distinct distortion in the C-terminal half of the peptide in a highly directional manner. The established synthetic route of [Ara3]CLV3 will greatly contribute to our understanding of the biology and biochemistry of arabinosylated peptide signals in plants.

  43. Biochemical mapping of a ligand-binding domain within Arabidopsis BAM1 reveals diversified ligand recognition mechanisms of plant LRR-RKs Reviewed

    Shinohara Hidefumi, Moriyama Yuji, Ohyama Kentaro, Matsubayashi Yoshikatsu

    PLANT JOURNAL   Vol. 70 ( 5 ) page: 845-854   2012.6

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    DOI: 10.1111/j.1365-313X.2012.04934.x

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  44. Recent progress in research on small post-translationally modified peptide signals in plants Reviewed

    Matsubayashi Yoshikatsu

    GENES TO CELLS   Vol. 17 ( 1 ) page: 1-10   2012.1

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1111/j.1365-2443.2011.01569.x

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  45. Biochemical mapping of a ligand-binding domain within Arabidopsis BAM1 reveals diversified ligand recognition mechanisms of plant LRR-RKs. Reviewed

    Shinohara H., Moriyama Y., Ohyama K., Matsubayashi Y.

    Plant J.   Vol. 70   page: 845-854   2012

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    Leucine-rich repeat receptor kinases (LRR-RKs) comprise the largest subfamily of the transmembrane receptor kinases in plants. In several LRR-RKs, a loop-out region called an island domain that intercepts the extracellular tandem LRRs at a position near the transmembrane domain constitutes the ligand-binding pocket, but the absence of the island domain in numerous LRR-RKs raises questions about which domain specifically recognizes the corresponding ligands in non-island domain-carrying LRR-RKs. Here, we determined by photoaffinity labeling followed by chemical and enzymatic digestion that BAM1, a CLV1/BAM family LRR-RK whose extracellular domain is comprised of 22 consecutive LRRs, directly interacts with the small peptide ligand CLE9 at the LRR6-8 region that is relatively distal from the transmembrane domain. Multiple sequence alignment and homology modeling revealed that the inner concave side of LRR6-8 of the CLV1/BAM family LRR-RKs is slightly deviatory from the LRR consensus. Supporting our findings, the clv1-4 mutant carries a missense mutation at the inner concave side of LRR6 of CLV1, and introduction of the corresponding mutation into BAM1 resulted in complete loss of ligand-binding activity. Our results indicate that ligand recognition mechanisms of plant LRR-RKs are more complex and diversified than anticipated.

  46. Post-Translational Modifications in Secreted Peptide Hormones in Plants Reviewed

    Matsubayashi Yoshikatsu

    PLANT AND CELL PHYSIOLOGY   Vol. 52 ( 1 ) page: 5-13   2011.1

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1093/pcp/pcq169

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  47. Arabinosylated glycopeptide hormones: new insights into CLAVATA3 structure Reviewed

    Shinohara Hidefumi, Matsubayashi Yoshikatsu

    CURRENT OPINION IN PLANT BIOLOGY   Vol. 13 ( 5 ) page: 515-519   2010.10

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    DOI: 10.1016/j.pbi.2010.05.008

    Web of Science

  48. Identification of tyrosylprotein sulfotransferase in Arabidopsis Reviewed

    Komori Ryota, Amano Yukari, Ogawa-Ohnishi Mari, Matsubayashi Yoshikatsu

    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA   Vol. 106 ( 35 ) page: 15067-15072   2009.9

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    DOI: 10.1073/pnas.0902801106

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  49. A glycopeptide regulating stem cell fate in Arabidopsis thaliana Reviewed

    Ohyama Kentaro, Shinohara Hidefumi, Ogawa-Ohnishi Mari, Matsubayashi Yoshikatsu

    NATURE CHEMICAL BIOLOGY   Vol. 5 ( 8 ) page: 578-580   2009.8

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    DOI: 10.1038/nchembio.182

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  50. Involvement of Phytosulfokine in the Attenuation of Stress Response during the Transdifferentiation of Zinnia Mesophyll Cells into Tracheary Elements Reviewed

    Motose Hiroyasu, Iwamoto Kuninori, Endo Satoshi, Demura Taku, Sakagami Youji, Matsubayashi Yoshikatsu, Moore Kevin L., Fukuda Hiroo

    PLANT PHYSIOLOGY   Vol. 150 ( 1 ) page: 437-447   2009.5

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    DOI: 10.1104/pp.109.135954

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  51. Non-cell-autonomous control of vascular stem cell fate by a CLE peptide/receptor system Reviewed

    Hirakawa Yuki, Shinohara Hidefumi, Kondo Yuki, Inoue Asuka, Nakanomyo Ikuko, Ogawa Mari, Sawa Shinichiro, Ohashi-Ito Kyoko, Matsubayashi Yoshikatsu, Fukuda Hiroo

    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA   Vol. 105 ( 39 ) page: 15208-15213   2008.9

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1073/pnas.0808444105

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  52. Identification of a biologically active, small, secreted peptide in Arabidopsis by in silico gene screening, followed by LC-MS-based structure analysis Reviewed

    Ohyama Kentaro, Ogawa Mari, Matsubayashi Yoshikatsu

    PLANT JOURNAL   Vol. 55 ( 1 ) page: 152-160   2008.7

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1111/j.1365-313X.2008.03464.x

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  53. Non-cell-autonomous control of vascular stem cell fate by a CLE peptide/receptor system. Reviewed

    Hirakawa Y., Shinohara H., Kondo Y., Inoue A., Nakanomyo I., Ogawa M., Sawa S., Ohashi-Ito K., Matsubayashi Y. and Fukuda H.

    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA   Vol. 105   page: 15208-15213   2008

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  54. Identification of a biologically active, small, secreted peptide in Arabidopsis by in silico gene screening, followed by LC-MS-based structure analysis. Reviewed

    Ohyama K, Ogawa M, Matsubayashi Y.

    Plant J.   Vol. 55   page: 152-160   2008

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    Peptidomics is a challenging field in which to create a link between genomic information and biological function through biochemical analysis of expressed peptides, including precise identification of post-translational modifications and proteolytic processing. We found that secreted peptides in Arabidopsis plants diffuse into the medium of whole-plant submerged cultures, and can be effectively identified by o-chlorophenol extraction followed by LC-MS analysis. Using this system, we first confirmed that a 12-amino-acid mature CLE44 peptide accumulated at a considerable level in the culture medium of transgenic plants overexpressing CLE44. Next, using an in silico approach, we identified a novel gene family encoding small secreted peptides that exhibit significant sequence similarity within the C-terminal short conserved domain. We determined that the mature peptide encoded by At1g47485, a member of this gene family, is a 15-amino-acid peptide containing two hydroxyproline residues derived from the conserved domain. This peptide, which we have named CEP1, is mainly expressed in the lateral root primordia and, when overexpressed or externally applied, significantly arrests root growth. CEP1 is a candidate for a novel peptide plant hormone.

  55. A tyrosine-sulfated glycopeptide involved in cellular proliferation and expansion in Arabidopsis. Reviewed

    Amano Yukari, Tsubouchi Hiroko, Shinohara Hidefumi, Ogawa Mari, Matsubayashi Yoshikatsu

    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA   Vol. 104 ( 46 ) page: 18333-18338   2007.11

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1073/pnas.0706403104

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  56. Functional immobilization of plant receptor-like kinase onto microbeads towards receptor array construction and receptor-based ligand fishing Reviewed

    Shinohara Hidefumi, Matsubayashi Yoshikatsu

    PLANT JOURNAL   Vol. 52 ( 1 ) page: 175-184   2007.10

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    DOI: 10.1111/j.1365-313X.2007.03204.x

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  57. Identification of ligand binding site of phytosulfokine receptor by on-column photoaffinity labeling Reviewed

    Shinohara Hidefumi, Ogawa Mari, Sakagami Youji, Matsubayashi Yoshikatsu

    JOURNAL OF BIOLOGICAL CHEMISTRY   Vol. 282 ( 1 ) page: 124-131   2007.1

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    DOI: 10.1074/jbc.M604558200

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  58. Identification of ligand binding site of phytosulfokine receptor by on-column photoaffinity labeling. Reviewed

    Shinohara H, Ogawa M, Sakagami Y, and Matsubayashi Y.

    J. Biol. Chem.   Vol. 282   page: 124-131   2007

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    Phytosulfokine (PSK), an endogenous 5-amino-acid secreted peptide in plants, affects cellular potential for growth via binding to PSKR1, a member of the leucine-rich repeat receptor kinase (LRR-RK) family. PSK interacts with PSKR1 in a highly specific manner with a nanomolar dissociation constant. However, it is not known which residues in the PSKR1 extracellular domain constitute the ligand-binding pocket. Here, we identified the PSK-binding domain of carrot PSKR1 (DcPSKR1) by photoaffinity labeling. We cross-linked the photoactivatable PSK analog [125I]Ne-[(4-azidosalicyl)Lys5]PSK with DcPSKR1 using UV irradiation, and mapped the cross-linked region using chemical and enzymatic fragmentation. We also established a novel メon-column photoaffinity labelingモ methodology that allows repeated incorporation of the photoaffinity label to increase the efficiency of the photoaffinity cross-linking reactions. We purified a labeled DcPSKR1 tryptic fragment using anti-PSK antibodies, and identified a peptide fragment that corresponds to the 15-amino-acid Glu503-Lys517 region of DcPSKR1 by MALDI-TOF MS. Deletion of Glu503-Lys517 completely abolishes the ligand-binding activity of DcPSKR1. This region is in the island domain flanked by extracellular LRRs, indicating that this domain forms a ligand-binding pocket that directly interacts with PSK.

  59. Functional immobilization of plant receptor-like kinase onto microbeads toward receptor array construction and receptor-based ligand fishing Reviewed

    Shinohara H. and Matsubayashi Y.

    Plant J.   Vol. 52   page: 175-184   2007

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    Despite the large number of receptor-like kinases (RLKs) in plants, few of them have known specific ligands. Ligand fishing is one of the most challenging post-genomic technologies. Here, we report a strategy for functional immobilization of plant RLKs on microbeads via covalent linkage. Due to the high density of immobilized RLK, ligand-receptor interaction can be visualized at high sensitivity using fluorescent-labeled ligands under the confocal laser scanning microscope. Moreover, using receptor-based affinity chromatography system with RLK microbeads, the ligand of the receptor was directly retrieved at high purity from complex natural samples. Our RLK microbeads and receptor-based ligand fishing approach is a feasible alternative to conventional forward genetics and bioassay-based biochemical purification for identification of novel ligand-receptor pairs in plants.

  60. Functional analysis of phytosulfokine receptor homologs in Arabidopsis Reviewed

    Tsubouchi Hiroko, Shinohara Hidefumi, Ogawa Mari, Sakagami Youji, Matsubayashi Yoshikatsu

    PLANT AND CELL PHYSIOLOGY   Vol. 48   page: S26-S26   2007

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  61. Identification of ligand binding site of phytosulfokine by on-column photoaffinity labeling Reviewed

    Shmohara Hidefumi, Ogawa Mari, Sakagami Youji, Matsubayashi Yoshikatsu

    PLANT AND CELL PHYSIOLOGY   Vol. 48   page: S26-S26   2007

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    Web of Science

  62. Disruption and overexpression of Arabidopsis phytosulfokine receptor gene affects cellular longevity and potential for growth Reviewed

    Matsubayashi Yoshikatsu, Ogawa Mari, Kihara Hitomi, Niwa Masaaki, Sakagami Youji

    PLANT PHYSIOLOGY   Vol. 142 ( 1 ) page: 45-53   2006.9

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    DOI: 10.1104/pp.106.081109

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  63. Disruption and overexpression of Arabidopsis phytosulfokine receptor gene affects cellular longevity and potential for growth. Reviewed

    Matsubayashi, Y., Ogawa, M., Kihara, H., Niwa, M., and Sakagami, Y.

    Plant Physiol.   Vol. 142   page: 45-53   2006

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    Phytosulfokine (PSK), a 5-amino-acid sulfated peptide that has been identified in conditioned medium of plant cell cultures, promotes cellular growth in vitro via binding to the membrane-localized PSK receptor. Here, we report that loss-of-function and gain-of-function mutations of the Arabidopsis PSK receptor (AtPSKR1) alter cellular longevity and potential for growth without interfering with basic morphogenesis of plants. Although mutant pskr1-1 plants exhibit morphologically normal growth until 3 weeks after germination, individual pskr1-1 cells gradually lose their potential to form calluses as tissues mature. Shortly after a pskr1-1 callus forms, it loses potential for growth, resulting in formation of a smaller callus than the wild-type. Leaves of pskr1-1 plants exhibit premature senescence after bolting. Leaves of AtPSKR1ox plants exhibit greater longevity and significantly greater potential for callus formation than leaves of wild-type plants, irrespective of their age. Calluses derived from AtPSKR1ox plants maintain their potential for growth longer than wild-type calluses. Combined with the present finding that PSK precursor genes are more strongly expressed in mature plant parts than in immature plant parts, the available evidence indicates that PSK signaling affects cellular longevity and potential for growth, and thereby exerts a pleiotropic effect on cultured tissue in response to environmental hormonal conditions.

  64. Identification and functional characterization of phytosulfokine receptor using a ligand-based approach Invited Reviewed

    Matsubayashi Y., Shinohara H. and Ogawa M.

    Chem. Rec.   Vol. 6   page: 356-364   2006

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  65. Peptide hormones in plants Invited

    Matsubayashi Y. and Sakagami Y.

    Annu. Rev. Plant Biol.   Vol. 57   page: 649-674   2006

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    In recent years, numerous biochemical and genetic studies have demonstrated that peptide signaling plays a greater than anticipated role in various aspects of plant growth and development. A substantial proportion of these peptides are secretory and act as local signals mediating cell-to-cell communication. Specific receptors for several peptides were identified as being membrane-localized receptor kinases, the largest family of receptor-like molecules in plants. These findings illustrate the importance of peptide signaling in the regulation of plant growth, functions that were previously ascribed to the combined action of small lipophilic compounds referred to as メtraditional plant hormones.モ Here, we outline recent advances in the current understanding of biologically active peptides in plants, currently regarded as a new class of plant hormones.

  66. Peptide hormones in plants Reviewed

    Matsubayashi Yoshikatsu, Sakagami Youji

    ANNUAL REVIEW OF PLANT BIOLOGY   Vol. 57   page: 649-674   2006

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    DOI: 10.1146/annurev.arplant.56.032604.144204

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  67. Identification and functional characterization of phytosulfokine receptor using a ligand-based approach Reviewed

    Matsubayashi Yoshikatsu, Shinohara Hidefumi, Ogawa Mari

    CHEMICAL RECORD   Vol. 6 ( 6 ) page: 356-364   2006

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    DOI: 10.1002/tcr.20090

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  68. Phytosulfokine Reviewed

    Matsubayashi Yoshikatsu, Sakagami Youji

    HANDBOOK OF BIOLOGICALLY ACTIVE PEPTIDES     page: 29-32   2006

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  69. Further analogues of plant peptide hormone phytosulfokine-alpha (PSK-alpha) and their biological evaluation Reviewed

    Bahyrycz A., Matsubayashi Y., Ogawa M., Sakagami Y. and Konopinska D.

    J. Pept Sci.   Vol. 11   page: 589-592   2005

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  70. Two stimulatory effects of the peptidyl growth factor phytosulfokine during somatic embryogenesis in Japanese larch (Larix leptolepis Gordon). Reviewed

    Umehara M., Ogita S., Sasamoto H., Eun CH., Matsubayashi Y., Sakagami Y. and Kamada H.

    Plant Sci.   Vol. 169   page: 901-907   2005

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  71. Ion-selective enrichment of tyrosine-sulfated peptides from complex protein digests. Reviewed

    Amano Y., Shinohara H., Sakagami Y. and Matsubayashi Y.

    Anal. Biochem.   Vol. 346   page: 124-131   2005

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    We have developed a novel procedure for concentrating sulfated peptides, as a front end to mass spectrometric analysis, based on ion-selective interaction of sulfate ions with anion exchangers. Ions with a higher charge and smaller solvated ion radius, such as sulfate ions, have higher retention in an ion exchanger, due to their greater degree of coulombic interactions. We tested the effectiveness of this approach for enrichment and identification of sulfated peptides, using a tryptic digest of bovine serum albumin spiked with model sulfated peptide (molar ratio, 20:1), and using a tryptic digest of bovine fibrinogen. Sulfated peptides are identified by mass spectrometry, in which both the molecular ion and its specific fragment ion produced by facile loss of SO3 are detected. In both experiments, sulfated peptides were strongly retained on the anion exchanger, and were eluted by higher concentrations of competing ion with minimum contamination of non-sulfated peptides. Using this procedure, we determined that the 13-amino-acid C-terminal peptide of the minor gユ chain of bovine fibrinogen contains sulfated tyrosine.

  72. Plant peptide hormone phytosulfokine (PSK-alpha) and its analogues Reviewed

    Bahyrycz Agata, Matsubayashi Yoshikatsu, Ogawa Mari, Sakagami Youji, Konopinska Danuta

    Peptides 2004, Proceedings     page: 784-785   2005

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  73. Chemical nursing: phytosulfokine improves genetic transformation efficiency by promoting the proliferation of surviving cells on selective media. Reviewed

    Matsubayashi Y., Goto T. and Sakagami Y.

    Plant Cell Rep.   Vol. 23   page: 155-158   2004

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  74. Plant peptide hormone phytosulfokine (PSK-alpha): synthesis of new analogues and their biological evaluation Reviewed

    Bahyrycz A., Matsubayashi Y., Ogawa M., Sakagami Y. and Konopinska D.

    J. Pept Sci.   Vol. 10   page: 462-469   2004

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  75. Phytosulfokine stimulates somatic embryogenesis in Cryptomeria japonica. Reviewed

    Igasaki T., Akashi N., Ujino-Ihara T., Matsubayashi Y., Sakagami Y. and Shinohara K.

    Plant Cell Physiol.   Vol. 44   page: 1412-1416   2003

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  76. Phytosulfokaine-α,a peptidyl plant grouth factor,stimulates cell cycle progression in carrot nonembryogenic cells.

    Plant Biotechnol   Vol. 20   page: 195-205   2003

  77. Ligand-receptor pairs in plant peptide signaling. Invited Reviewed

    Matsubayashi Y.

    J. Cell Sci.   Vol. 116   page: 3863-3870   2003

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  78. 植物細胞の増殖を制御するペプチド

    松林嘉克

        2001.7

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    "Peptide Newsletter Japan, 41, 5-7"

  79. ペプチド性植物細胞増殖因子に関する研究(総説).

    松林嘉克

    日本農芸化学会誌   Vol. 75   page: 1275-1281   2001.1

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  80. Peptide signals and their receptors in higher plant.

    "Matsubayashi Y., Yang H. and Sakagami Y."

    Trends in Plant Science   Vol. 6   page: 573-577   2001.1

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  81. Diversity of arabidopsis genes encoding precursors for phytosulfokine,a peptide growth factor. Reviewed

    Plant Physiol.   Vol. 127   page: 842-851   2001

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  82. Peptide signals and their receptors in higher plants

    Trends in Plant Science   Vol. 6   page: 573-577   2001

  83. Peptide growth factor phytosulfokine-a contributes to pollen population effect.

    "Chen Y.F., Matsubayashi Y. and Sakagami Y."

    Planta   Vol. 211   page: 752-755   2000.1

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  84. Existence of a plant tyrosylprotein sulfotransferase: novel plant enzyme catalyzing tyrosine O-sulfation of preprophytosulfokine variants in vitro.

    "Hanai H., Nakayama D., Yang H., Matsubayashi Y., Hirota Y. and Sakagami Y."

    FEBS Letters   Vol. 470   page: 97-101   2000.1

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  85. "Rapid and efficient Agrobacterium infection-mediated transient gene expression in rice Oc cells and its application for expression and suppression of OsPSK cDNA for a precursor to phytosulfokine-a, a peptide plant growth factor."

    "Yang H., Morita A., Matsubayashi Y., Nakamura K. and Sakagami Y."

    Plant and Cell Physiology   Vol. 41   page: 811-816   2000.1

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  86. "Molecular cloning and characterization of OsPSK, a gene encoding a precursor for phytosulfokine-α, required for rice cell proliferation."

    "Yang H., Matsubayashi Y., Hanai H., Nakamura K. and Sakagami Y."

    Plant Molecular Biology   Vol. 44   page: 635-647   2000.1

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  87. "Phytosulfokine-α, a peptide growth factor found in higher plants: Its structure, functions, precursor and receptors."

    "Yang H., Matsubayashi Y., Hanai H. and Sakagami Y."

    Plant and Cell Physiology   Vol. 41   page: 825-830   2000.1

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  88. 120- and 160-kDa receptors for endogenous mitogenic peptide, phytosulfokine-alpha, in rice plasma membranes. Reviewed

    J. Biol. Chem.   Vol. 275   page: 15520-15525   2000

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  89. A secreted peptide growth factor, phytosulfokine,acting as a stimulatory factor of carrot somatic embryo formation. Reviewed

    Plant Cell Physiol.   Vol. 41   page: 27-32   2000

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  90. "Promotive effects of the peptidyl plant growth factor, phytosulfokine-a, on the growth and chlorophyll content of Arabidopsis seedlings under high night-time temperature conditions."

    "Yamakawa S., Matsubayashi Y. Sakagami Y., Kamada H. and Satoh S."

    "Bioscience, Biotechnology and Biochemistry"   Vol. 63   page: 2240-2243   1999.1

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  91. "Stimulatory effects of a novel peptidyl plant growth factor, phytosulfokine-a, on the adventitious bud formation from callus of Antirrhium majus."

    "Yang G., Shen T., Kobayashi Y., Matsubayashi Y., Sakagami Y. and Kamada H."

    Plant Biotechnology   Vol. 16   page: 231-234   1999.1

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  92. Oryza sativa PSK gene encodes a precursor ofphytosulfokine-alpha, a sulfated peptide growthfactor found in plants. Reviewed

    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA   Vol. 96   page: 13560-13565   1999

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  93. Physiological relationships between auxin, cytokinin, and a peptide growth factor, phytosulfokine-α, in stimulation of asparagus cell proliferation.

    Planta   Vol. 207   page: 559-565   1999

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  94. Phytosulfokine-alpha, a peptidyl plant growth factor, stimulates somatic embryogenesis in carrot. Reviewed

    J. Exp. Bot.   Vol. 50   page: 1123-1128   1999

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  95. The endogenous sulfated pentapeptide, phytosulfokine-alpha, stimulates tracheary element differentiation of isolated mesophyll cells of Zinnia elegans. Reviewed

    Plant Physiol.   Vol. 120   page: 1043-1048   1999

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  96. Characterization of specific binding sites for a mitogenic sulfated peptide, phytosulfokine-alpha, in the plasma membrane fraction derived from Oryza sativa L. Reviewed

    Eur. J. Biochem.   Vol. 262   page: 666-671   1999

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  97. Preparation and characterization of fully active biotinylated analogs of phytosulfokine-alpha. Reviewed

    Biosci. Biotechnol. Biochem.   Vol. 63   page: 1847-1849   1999

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  98. "The promotive effects of a peptidyl plant growth factor, phytosulfokine-α,on the formation of adventitious roots and expression of a gene for a root-specific cystain in cucumber hypocotyls."

    "Yamakawa S., Sakuta C., Matsubayshi Y., Sakagami Y., Kamada H. and Satoh S."

    Journal of Plant Research   Vol. 111   page: 453-458   1998.1

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  99. Differential induction by methyl jasmonate of genes encoding ornithine decarboxylase and other enzymes involved in nicotine biosynthesis in tobacco cell cultures.

    "Imanishi S., Hashizume K., Nakakita M., Kojima H., Matsubayashi Y., Hashimoto T., Sakagami Y., Yamada Y. and Nakamura K."

    Plant Molecular Biology   Vol. 38   page: 1101-1111   1998.1

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  100. Promotion by a peptidyl growth factor, phytosulfokine-α, of chlorophyll formation in etiolated cotyledon of cucumber

    Yamakawa S., Matsubayashi Y., Sakagami Y., Kamada H. and Satoh S.

    Biosci. Biotech. Biochem.   Vol. 62   page: 2441-2443   1998

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  101. Effect of medium ammonium-nitrate ratio on competence for cell division induced by phytosulfokine-alpha. Reviewed

    Plant Cell Rep.   Vol. 17   page: 368-372   1998

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  102. 植物細胞の増殖を誘導するペプチド性因子.

    "松林嘉克,坂神洋次"

    化学と生物   Vol. 35   page: 6-8   1997.1

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  103. 植物細胞増殖因子(総説).

    "松林嘉克,坂神洋次"

    植物の化学調節   Vol. 32   page: 160-171   1997.1

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  104. Phytosulfokine-alpha, a sulfated pentapeptide, stimulates the proliferation of rice cells by means of specific high-and low-affinity binding sites. Reviewed

    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA   Vol. 94   page: 13357-13362   1997

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  105. 植物細胞増殖因子(ミニレビュー).

    "松林嘉克,坂神洋次"

    日本農芸化学会誌   Vol. 70   page: 588-590   1996.1

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  106. Phytosulfokine, sulfated peptides that induce the proliferation of single cells of Asparagus officinalis L. Reviewed

    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA   Vol. 93   page: 7623-7627   1996

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  107. Active fragments and analogs of the plaant growth factor, phytosulfokine : Structure-activity relationships. Reviewed

    Biochem. Biophys. Res. Commun.   Vol. 225   page: 209-214   1996

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▼display all

Books 7

  1. 植物の行動生態学

    松林嘉克( Role: Joint author ,  第7章 長距離移行ペプチドを介した植物の変動窒素環境への適応)

    種生物学会  2023.1 

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    Language:Japanese Book type:Scholarly book

  2. Evaluation of direct ligand-receptor interactions by photoaffinity labeling Reviewed

    H Shinohara, Y Matsubayashi( Role: Joint author)

    Plant Peptide Hormones and Growth Factors  2023 

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    Responsible for pages:231-240   Language:English Book type:Scholarly book

  3. Extraction of apoplastic peptides for the structural elucidation of mature peptide hormones in Arabidopsis Reviewed

    M Ogawa-Ohnishi, Y Matsubayashi( Role: Joint author)

    Plant Peptide Hormones and Growth Factors  2023 

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    Responsible for pages:81-87   Language:English Book type:Scholarly book

  4. 植物の低窒素環境における生存戦略

    大久保祐里,松林嘉克,木羽隆敏( Role: Joint author)

    化学と生物/日本農芸化学会  2022.7 

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    Responsible for pages:352-359   Language:Japanese Book type:Scholarly book

  5. 植物の窒素吸収を制御する長距離情報伝達 −葉と根のコミュニケーションによる需要と供給の調節機構−

    大久保祐里,松林嘉克( Role: Joint author)

    情報処理/情報処理学会  2021.11 

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    Responsible for pages:e10-e12   Language:Japanese Book type:Scholarly book

  6. 植物の窒素栄養吸収制御の巧みなしくみ

    大久保祐里,松林嘉克( Role: Joint author)

    生化学/日本生化学会  2021.6 

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    Responsible for pages:400-403   Language:Japanese Book type:Scholarly book

  7. 窒素取り込みを制御する長距離移行ペプチド群

    大久保祐里,松林嘉克( Role: Joint author)

    アグリバイオ/北隆館  2021.5 

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    Language:Japanese Book type:Scholarly book

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Presentations 22

  1. 葉と根を行き交うペプチドシグナルによる変動する窒素環境への適応 Invited

    大久保祐里,松林嘉克

    第65回日本植物生理学会年会  2024.3.17  日本植物生理学会

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    Event date: 2024.3

    Language:Japanese   Presentation type:Symposium, workshop panel (nominated)  

    Venue:神戸  

  2. 細菌由来の免疫誘導ペプチドflg22のC末端を切り出す植物プロテアーゼの同定

    松井 彩,松林嘉克

    第65回日本植物生理学会年会  2024.3.17  日本植物生理学会

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    Event date: 2024.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:神戸  

  3. Plant adaptation to fluctuating nitrogen environments by long-range mobile peptide signals Invited International conference

    Nitrogen2023  2023.11.8 

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    Event date: 2023.11

    Language:English   Presentation type:Oral presentation (keynote)  

  4. Shoot-derived polypeptides regulate nitrate uptake in the roots under fluctuating nitrogen environments International conference

    Nitrogen2023  2023.11.9 

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    Event date: 2023.11

    Language:English   Presentation type:Oral presentation (general)  

  5. Plant adaptation to fluctuating nitrogen environments by long-range mobile peptide signals Invited International conference

    11th Plant Peptides and Receptors Meeting  2023.10.3 

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    Event date: 2023.10

    Language:English   Presentation type:Oral presentation (keynote)  

  6. 植物の硝酸吸収活性を制御するリン酸化/脱リン酸化酵素の解析

    大久保祐里,松林嘉克

    日本植物学会第87回大会  2023.9.7  日本植物学会

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    Event date: 2023.9

    Language:Japanese   Presentation type:Poster presentation  

    Venue:札幌  

  7. 転写因子TGAと長距離移行ペプチドによる硝酸吸収の統御メカニズム

    大久保祐里,松林嘉克

    植物の栄養研究会 第8回研究交流会  2023.9.5  植物の栄養研究会 第8回研究交流会

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    Event date: 2023.9

    Language:Japanese   Presentation type:Poster presentation  

    Venue:札幌  

  8. Exploring peptide hormones in plants: Identification of five peptide hormone-receptor pairs and two post-translational modification enzymes Invited International conference

    The 24th International Conference on Plant Growth Substances  2023.7.4 

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    Event date: 2023.7

    Language:English   Presentation type:Oral presentation (invited, special)  

    Venue:Gyeongju, Korea  

  9. Peptide signal-mediated adaptation to spatially and temporally flucuating environments in plants Invited International conference

    The 33rd International Conference on Arabidopsis Research  2023.6.9 

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    Event date: 2023.6

    Language:English   Presentation type:Oral presentation (keynote)  

  10. Regulation of high-affinity nitrate uptake activity by NRT2.1 phosphorylation Invited International conference

    The 33rd International Conference on Arabidopsis Research  2023.6.9 

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    Event date: 2023.6

    Language:English   Presentation type:Poster presentation  

  11. AGP polysaccharide chains are required for normal biogenesis of plasmodesmata Invited International conference

    The 33rd International Conference on Arabidopsis Research  2023.6.9 

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    Event date: 2023.6

    Language:English   Presentation type:Poster presentation  

  12. 植物のストレス応答と成長のトレードオフを制御するペプチドホルモン Invited

    大西(小川)真理,山下智偉,垣田満,中山卓也,大久保祐里,林陽子,山下恭子,野村泰三,野田沙希,篠原秀文,松林嘉克

    第64回日本植物生理学会年会  2023.3  日本植物生理学会

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    Event date: 2023.3

    Language:Japanese   Presentation type:Symposium, workshop panel (nominated)  

    Venue:京都  

  13. Peptide ligand-mediated trade-off between plant growth and stress response Invited International conference

    松林嘉克

    38th IPSR International Symposium  2023.2.27  岡山大学資源植物科学研究所

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    Event date: 2023.2

    Language:English   Presentation type:Symposium, workshop panel (nominated)  

    Venue:岡山   Country:Japan  

  14. 定量リン酸化プロテオミクスを用いた植物プロテインフォスファターゼの基質探索 Invited

    松林嘉克,大久保祐里

    第95回日本生化学会大会シンポジウム  2022.11.9  日本生化学会

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    Event date: 2022.11

    Language:Japanese   Presentation type:Symposium, workshop panel (nominated)  

    Venue:名古屋  

  15. 葉と根を行き交うペプチド群による植物の窒素吸収制御 Invited

    大久保祐里,松林嘉克

    第95回日本生化学会大会シンポジウム  2022.11.9  日本生化学会

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    Event date: 2022.11

    Language:Japanese   Presentation type:Symposium, workshop panel (nominated)  

    Venue:名古屋  

  16. Plant Receptor Kinases Involved in Perception of Peptide Hormones Invited International conference

    松林嘉克

    International Conference of the Korean Society for Molecular and Cellular Biology 2022  2022.9.29  Korean Society for Molecular and Cellular Biology

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    Event date: 2022.9

    Language:English   Presentation type:Symposium, workshop panel (nominated)  

    Venue:済州島   Country:Korea, Republic of  

  17. 植物の窒素吸収を制御する長距離シグナル伝達 Invited

    大久保祐里,松林嘉克

    日本植物学会大86回大会シンポジウム  2022.9  日本植物学会

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    Event date: 2022.9

    Language:Japanese   Presentation type:Symposium, workshop panel (nominated)  

    Venue:京都  

  18. 植物の窒素吸収を制御する長距離移行ペプチド群 Invited

    大久保祐里,松林嘉克

    第44回日本分子生物学会年会ワークショップ 「不均一環境変動に対する植物の情報統御機構」  2021.12.3  日本分子生物学会

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    Event date: 2021.12

    Language:Japanese   Presentation type:Symposium, workshop panel (nominated)  

    Venue:横浜  

  19. 理研バイオリソースに支えられた植物ペプチドホルモン研究 Invited

    松林嘉克

    理研BRC設立20周年記念シンポジウム  2021.10.20  理化学研究所

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    Event date: 2021.10

    Language:Japanese   Presentation type:Symposium, workshop panel (nominated)  

  20. Roles of two structurally distinct sulfated peptides, PSK and PSY1, in Arabidopsis growth and development International conference

    19th International Conference on Plant Growth Substances 

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    Event date: 2007.7

    Language:English   Presentation type:Oral presentation (invited, special)  

  21. Physiological functions of two structurally distinct tyrosine-sulfated peptides in Arabidopsis growth and development International conference

    18th International Conference on Arabidopsis Research 

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    Event date: 2007.6

    Language:English   Presentation type:Oral presentation (invited, special)  

  22. An LRR receptor kinase involved in the perception of a peptide plant hormone, phytosulfokine

    Protein Phosphorylation in Plant Signaling 2005 

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    Event date: 2005.10

    Language:English   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

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Research Project for Joint Research, Competitive Funding, etc. 1

  1. 新規ペプチドリガンド-受容体ペアの探索を基軸とした植物成長の分子機構解析

    2010.2 - 2013.5

    先端研究助成基金助成金(最先端・次世代研究開発支援プログラム) 

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    Grant type:Competitive

    本研究は,ホルモンなどの物質による植物成長の人為的制御を目指して,新しい植物ホルモンやその受容体を見つけ出すことを主たる目的とした.研究の結果,根端メリステムの細胞分裂活性の制御に関与する新しいペプチドホルモンの発見とその受容体の同定,側根形成への関与が示唆されるペプチドの受容体の同定,マメ科植物の根粒数の調節に関与する糖ペプチドの構造解明と受容体への結合の証明などの成果を得た.また,一部のペプチドやタンパク質の活性化に必要なヒドロキシプロリン残基のアラビノシル化修飾を担う糖転移酵素の精製・同定に成功した.いずれもこれまで全く知られていなかった新しい分子やしくみの発見であり,農作物の品種改良や生育調節に応用可能であると考えられる.

KAKENHI (Grants-in-Aid for Scientific Research) 11

  1. Dissection of plant growth and environmental response mechanisms through peptide signaling

    Grant number:23H05477  2023.4 - 2028.3

    Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (S)

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    Authorship:Principal investigator  Grant type:Competitive

    Grant amount:\202930000 ( Direct Cost: \156100000 、 Indirect Cost:\46830000 )

  2. Multi-layered regulatory system of plant resilience under fluctuating environment

    Grant number:20H05905  2020.11 - 2025.3

    Grants-in-Aid for Scientific Research  Grant-in-Aid for Transformative Research Areas (A)

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    Authorship:Coinvestigator(s)  Grant type:Competitive

  3. 長距離シグナリングを介した不均一環境変動への適応機構

    Grant number:20H05907  2020.11 - 2025.3

    科学研究費助成事業  学術変革領域研究(A)

    松林 嘉克, 桑田啓子

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    Authorship:Principal investigator  Grant type:Competitive

    Grant amount:\60060000 ( Direct Cost: \46200000 、 Indirect Cost:\13860000 )

    動き回ることのできない植物は土壌中の窒素などの栄養源に代表されるような空間的に不均一な環境に適応する必要があり、また光合成を行なう気相と栄養吸収を行なう土相という異なる環境空間にまたがって生育するため、地上部と地下部の双方において変動する環境情報を時空間的に統合して適応する必要に迫られる。本研究では、これらの不均一変動環境への適応過程に関わる長距離シグナル伝達に着目し、その分子群の同定や情報統御メカニズムの解明を目指す。
    動き回ることのできない植物は,土壌中の窒素などの栄養源に代表されるような空間的に不均一な環境に適応する必要がある.また光合成を行なう気相と栄養吸収を行なう土相という異なる環境空間にまたがって生育するため,地上部と地下部の双方において変動する環境情報を時空間的に統合して適応する必要に迫られる.本研究では,これらの不均一変動環境への適応過程に関わる長距離シグナル伝達に着目し,その分子群の同定や情報統御メカニズムの解明を目指している.
    1.葉から根に移行する窒素要求シグナルCEPD/CEPDL2が,どのように根でターゲット遺伝子群の発現を誘導するのかを明らかにするために,CEPDL2-GFPをベイトとしたCo-IP/MS解析を行なった.その結果,互いにファミリーの関係にある2個の転写因子が単離されたため,機能解析や欠損株の表現型解析を進めている.
    2.CEPD/CEPDL2ペプチドはシロイヌナズナで21個の遺伝子からなるファミリーを形成しているが,その中にCEPDとは逆に過剰発現で窒素吸収を抑制する分子群を見出した.多重欠損株を作成して表現型の観察を進めている.
    3.窒素欠乏の根におけるCEP誘導のメカニズムを含めて,根が窒素欠乏をどのように感知しているかはよくわかっていない.そこで,15Nを用いた安定同位体標識法による定量リン酸化プロテオミクスを行なった.15分間の窒素欠乏処理でリン酸化変動する初期応答タンパク質群を複数同定したため,機能解析を進めている.
    全身的窒素要求シグナリングでは,これまでに道管移行ペプチドCEP-受容体CEPR-篩管移行ペプチドCEPD-フォスファターゼCEPHなどの主要コンポーネントが明らかになっているが,①窒素欠乏の根におけるCEP誘導のメカニズム,②葉におけるCEPR活性化からCEPD誘導までの分子機構,③根に移行したCEPDが根圏の窒素状況依存的に硝酸取り込みを活性化するメカニズム,などについてはまだ明らかになっていない.それぞれに関わる分子群を見出すことは全身的窒素要求シグナリングを理解する上で不可欠である.
    今回の結果から,CEPD/CEPDL2は根で転写因子と直接結合し,転写補助因子として下流遺伝子群の発現を制御していることが明らかになった.この知見は,③のメカニズムを解明する上で,大きな前進である.また,CEPD/CEPDL2とは逆に葉の窒素十分を根に伝えるシグナルの存在も示されたことから,植物体の窒素恒常性は,両者のバランスで維持されている可能性が高い.また,①と②の解析では,トランスクリプトームやリン酸化プロテオミクスによってシグナリングに関与する分子群の探索を進めており,得られた候補分子について欠損株の表現型観察やCEP応答性を解析している.
    「現在までの進捗状況」に記した①から③の各項目の研究を引き続き進めていく.特にCEPD/CEPDL2が結合する転写因子が見出されたことは,根に移行したCEPDが硝酸取り込みを活性化するメカニズムの解明に大きな前進であるため,転写因子のChIP-seqなど,標的遺伝子群の同定も含めて研究を進めていく.

  4. Integrative system of autonomous environmental signal recognition and memorization for plant plasticity

    Grant number:20H05603  2020.4 - 2021.3

    Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

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    Authorship:Coinvestigator(s) 

  5. Molecular dissection of peptide signaling in plants

    Grant number:18H05274  2018.6 - 2023.3

    Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (S)

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    Authorship:Principal investigator 

    Grant amount:\192530000 ( Direct Cost: \148100000 、 Indirect Cost:\44430000 )

  6. Integrative system of autonomous environmental signal recognition and memorization for plant plasticity

    Grant number:15H05955  2015.6 - 2020.3

    Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

    Toshinori Kinoshita

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    Authorship:Coinvestigator(s) 

    This new area of scientific study began with the goal of performing an integrated analysis of the dynamic environmental response control system in plants. This analysis includes clarifying the flexible and ingenious local and autonomous environmental response system evolved by immobile plants, which is completely different from those of animals, as well as a new spatiotemporal perspective. Specifically, we seek to analyze the long-distance signal transduction system of plants, as well as the environmental memory system that caches information via chromatin modifications. Actually, we have obtained exciting results regarding the dynamic environmental response control system in plants.

  7. Response mechanisms to a fluctuating environment through long-distance signaling

    Grant number:15H05957  2015.6 - 2020.3

    Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

    Matsubayashi Yoshikatsu

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    Authorship:Principal investigator 

    Grant amount:\95940000 ( Direct Cost: \73800000 、 Indirect Cost:\22140000 )

    Nitrogen (N) is an essential macronutrient that plays a crucial role throughout plant development, but soil nitrate availability fluctuates temporally and spatially. We identified that N starvation-induced peptide CEP acts as a root-derived ascending N-demand signal. CEP is recognized in the shoot by CEP receptor, that leads to the production of non-secreted polypeptide, CEPD. CEPD acts as a descending signal to the root and ultimately upregulates nitrate transporter NRT2.1 gene in the roots. Thus, CEP induced on one side of the roots by local N starvation is able to mediate upregulation of NRT2.1 in the distant part of the roots. In addition to this, plants modulate the efficiency of root N acquisition in response to shoot N demand by CEPD-like 2, which is upregulated in the leaf vasculature in response to decreased shoot N status. Our findings provide new insights into the molecular basis of plant adaptation to a dynamically fluctuating N environment.

  8. Molecular dissection of plant development and cell-to-cell signaling mediated by posttranslationally modified peptides

    Grant number:25221105  2013.5 - 2018.3

    Matsubayashi Yoshikatsu

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    Authorship:Principal investigator 

    Grant amount:\209820000 ( Direct Cost: \161400000 、 Indirect Cost:\48420000 )

    Our goal in this project is to uncover the mechanisms by which plant development is regulated through identification of novel peptide hormones and their specific receptors. In plants, small post-translationally modified peptides constitute the largest group of peptide hormones. We explored this type of peptide hormone by in silico gene screening coupled with biochemical and functional analysis, which led to the identification of two types of novel peptide hormones such as CEP family peptides regulating long-distance nitrogen demand signaling and CIF family peptides required for contiguous Casparian strip formation in roots. We also identified receptors for RGF family peptides involved in root meristem development.

  9. 分泌因子および受容体キナーゼを介した情報伝達機構

    2007.9 - 2010.1

    科学研究費補助金  特定領域研究

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    Authorship:Principal investigator 

    ペプチドホルモンの翻訳後修飾のひとつであるチロシン硫酸化に関与する酵素(tyrosylprotein sulfotransferase: TPST)の同定に成功し,この酵素の欠損株では,根端の幹細胞が維持されず,根が極端に短くなることを見出した.さらに,この表現型の原因のひとつとなっていた新しいチロシン硫酸化ペプチドホルモン(root meristem growth factor: RGF)の同定にも成功した.RGFは,根端メリステム幹細胞ニッチの維持およびメリステム活性の制御に関与している.また,液体培地に分泌された微量の分泌型ペプチドの効率的な抽出・構造解析法を確立し,新規ペプチドホルモンの候補を複数見出した.この手法を応用したCLV3ペプチドの構造解析の過程で,Hyp側鎖への糖鎖付加(トリアラビノシル化)という翻訳後修飾を見出し,この修飾がCLV3の生物活性や受容体結合活性を高める重要な働きをしていることを突き止めた.

  10. 翻訳後修飾に着目した分泌型ペプチドミクス

    2006.4 - 2009.3

    科学研究費補助金  若手研究(A)

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    Authorship:Principal investigator 

    植物のかたちづくりには様々な内生のペプチドホルモンが関与している.本研究では,in silicoおよび生化学的解析を統合して,翻訳後修飾ペプチドホルモン候補を探索し,その生理機能解析を行なった.その結果,複数の新規ペプチドホルモン候補を見出すことに成功した.また,翻訳後修飾のひとつであるチロシン硫酸化酵素の精製とクローニングを行ない,その遺伝子破壊株が様々な成長異常を示すことを見出した.

  11. 高等植物におけるタンパク質チロシン硫酸化機構の解明

    2004.4 - 2006.3

    科学研究費補助金  若手研究(B)

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    Authorship:Principal investigator 

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Teaching Experience (On-campus) 2

  1. 生物学基礎I

    2022

  2. 生理活性物質化学

    2009

Teaching Experience (Off-campus) 5

  1. 大学院特別講義

    2023.12 Kyushu University)

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    Level:Postgraduate 

  2. 大学院特別講義

    2023.9 Okayama University)

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    Level:Postgraduate 

  3. 大学院特別講義

    2022.12 Kyoto University)

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    Level:Postgraduate 

  4. 大学院特別講義

    2007.4 - 2008.3 東京大学大学院農学生命科学研究科)

  5. 大学院特別講義

    2006.4 - 2007.3 京都大学大学院理学研究科)

 

Social Contribution 1

  1. 新規ペプチドホルモンの発見から明らかになる植物の巧みな成長・環境応答機構

    Role(s):Appearance

    愛知県立岡崎高等学校・あいち科学技術教育推進協議会  科学三昧inあいち2022  2022.12

Media Coverage 4

  1. 植物の成長とストレス応答を制御 Newspaper, magazine

    日経産業新聞  日経産業新聞  2022.10

  2. 名古屋大が新ホルモン発見 Newspaper, magazine

    科学新聞  科学新聞  2022.10

  3. ストレスに応じ植物の成長調整 Newspaper, magazine

    読売新聞  読売新聞  夕刊  2022.10

  4. 成長を続けるかストレス防御か 植物の切り替えホルモン発見 Newspaper, magazine

    中日新聞  中日新聞  朝刊  2022.10