Updated on 2026/03/25

写真a

 
MATSUBAYASHI Yoshikatsu
 
Organization
Graduate School of Science Professor
Graduate School
Graduate School of Science
Undergraduate School
School of Science Department of Biological Science
Title
Professor
Contact information
メールアドレス
Homepage
http://www.bio.nagoya-u.ac.jp/~b2
External link

Degree 1

  1. 博士（農学） （ 1997.10   名古屋大学 ） 

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Research Interests 10

  1. ペプチドホルモン

  2. リガンド

  3. 翻訳後修飾

  4. 分泌型ペプチド

  5. 受容体

  6. 細胞間情報伝達

  7. 翻訳後修飾

  8. 受容体キナーゼ

  9. ペプチドホルモン

  10. シロイヌナズナ

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Research Areas 3

  1. Others / Other  / Plant Physiology

  2. Others / Other  / Biological Production Chemistry/Biological Organic Chemistry

  3. Life Science / Plants: molecular biology and physiology

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Current Research Project and SDGs 1

  1. 細胞間シグナルと受容体の解析から探る植物のかたちづくりと環境応答

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Research History 8

  1. Nagoya University   Professor

    2014.4

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    Country：Japan

  2. Nagoya University   Graduate School of Science, Division of Biological Science   Professor

    2014.4

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  3. 自然科学研究機構　基礎生物学研究所   教授

    2011.1 - 2014.3

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    Country：Japan

  4. Nagoya University   Associate Professor

    2007.4 - 2010.12

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    Country：Japan

  5. Nagoya University   Graduate School of Bioagricultural Sciences Department of Applied Biosciences   Assistant Professor

    2007.3

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  6. Nagoya University   Associate Professor

    2002.4 - 2007.3

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    Country：Japan

  7. Nagoya University   Graduate School of Bioagricultural Sciences, Department of Applied Biosciences   Assistant Professor

    2002.4

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  8. Nagoya University   Assistant

    1999.1 - 2002.3

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    Country：Japan

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Education 2

  1. Nagoya University

    - 1997.10

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    Country： Japan

  2. Nagoya University   Faculty of Agriculture

    - 1993.3

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    Country： Japan

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Professional Memberships 9

  1. 日本植物生理学会

  2. 日本分子生物学会

  3. 植物化学調節学会

  4. 日本植物学会

  5. 日本農芸化学会

  6. THE JAPANESE SOCIETY OF PLANT PHYSIOLOGISTS

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  7. THE JAPANESE SOCIETY FOR CHEMICAL REGULATION OF PLANTS

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  8. JAPAN SOCIETY FOR BIOSCIENCE, BIOTECHNOLOGY, AND AGROCHEMISTRY

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  9. THE MOLECULAR BIOLOGY SOCIETY OF JAPAN

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Committee Memberships 2

  1. 日本植物生理学会   代議員  

    2026.3 - 2027.3   

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    Committee type：Academic society

  2. 科学技術振興機構   創発アドバイザー  

    2023.10   

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    Committee type：Academic society

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Awards 11

  1. 日本植物生理学会賞

    2026.3   日本植物生理学会  

    松林嘉克

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    Award type：Award from Japanese society, conference, symposium, etc.  Country：Japan

  2. Silver Medal

    2019.7   The International Plant Growth Substances Association  

    松林 嘉克

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  3. IPGSA Silver Medal

    2019.6   International Plant Growth Substances Association  

    松林嘉克

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    Award type：Award from international society, conference, symposium, etc.  Country：Japan

  4. 読売テクノフォーラム・ゴールドメダル賞

    2019.5   読売テクノフォーラム  

    松林嘉克

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    Award type：Award from publisher, newspaper, foundation, etc.  Country：Japan

  5. ゴールドメダル賞

    2019.5   読売テクノフォーラム  

    松林 嘉克

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  6. 井上学術賞

    2019.2   井上科学振興財団  

    松林嘉克

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    Award type：Award from publisher, newspaper, foundation, etc.  Country：Japan

  7. 井上学術賞

    2019.2   井上科学振興財団  

    松林 嘉克

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  8. 日本学術振興会賞

    2016.2   日本学術振興会  

    松林嘉克

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    Award type：Award from publisher, newspaper, foundation, etc.  Country：Japan

  9. 日本分子生物学会三菱化学奨励賞

    2010.12   日本分子生物学会  

    松林嘉克

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    Award type：Award from Japanese society, conference, symposium, etc.  Country：Japan

  10. 日本植物生理学会奨励賞

    2008.3   日本植物生理学会  

    松林嘉克

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    Country：Japan

  11. 農芸化学奨励賞

    2001.3   日本農芸化学会  

    松林嘉克

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    Country：Japan

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Papers 116

  1. Integration of shoot-derived polypeptide signals by root TGA transcription factors is essential for survival under fluctuating nitrogen environments. Reviewed International journal Open Access

    Ryutaro Kobayashi, Yuri Ohkubo, Mai Izumi, Ryosuke Ota, Keiko Yamada, Yoko Hayashi, Yasuko Yamashita, Saki Noda, Mari Ogawa-Ohnishi, Yoshikatsu Matsubayashi

    Nature communications   Vol. 15 ( 1 ) page： 6903 - 6903   2024.8

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    Unlike plants in the field, which experience significant temporal fluctuations in environmental conditions, plants in the laboratory are typically grown in controlled, stable environments. Therefore, signaling pathways evolved for survival in fluctuating environments often remain functionally latent in laboratory settings. Here, we show that TGA1 and TGA4 act as hub transcription factors through which the expression of genes involved in high-affinity nitrate uptake are regulated in response to shoot-derived phloem mobile polypeptides, CEP DOWNSTREAM 1 (CEPD1), CEPD2 and CEPD-like 2 (CEPDL2) as nitrogen (N) deficiency signals, and Glutaredoxin S1 (GrxS1) to GrxS8 as N sufficiency signals. CEPD1/2/CEPDL2 and GrxS1-S8 competitively bind to TGA1/4 in roots, with the former acting as transcription coactivators that enhance the uptake of nitrate, while the latter function as corepressor complexes together with TOPLESS (TPL), TPL-related 1 (TPR1) and TPR4 to limit nitrate uptake. Arabidopsis plants deficient in TGA1/4 maintain basal nitrate uptake and exhibit growth similar to wild-type plants in a stable N environment, but are impaired in regulation of nitrate acquisition in response to shoot N demand, leading to defective growth under fluctuating N environments where rhizosphere nitrate ions switch periodically between deficient and sufficient states. TGA1/4 are crucial transcription factors that enable plants to survive under fluctuating and challenging N environmental conditions.

    DOI： 10.1038/s41467-024-51091-5

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  2. Arabidopsis SBT5.2 and SBT1.7 subtilases mediate C-terminal cleavage of flg22 epitope from bacterial flagellin. Reviewed International journal Open Access

    Sayaka Matsui, Saki Noda, Keiko Kuwata, Mika Nomoto, Yasuomi Tada, Hidefumi Shinohara, Yoshikatsu Matsubayashi

    Nature communications   Vol. 15 ( 1 ) page： 3762 - 3762   2024.5

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    Plants initiate specific defense responses by recognizing conserved epitope peptides within the flagellin proteins derived from bacteria. Proteolytic cleavage of epitope peptides from flagellin by plant apoplastic proteases is thought to be crucial for the perception of the epitope by the plant receptor. However, the identity of the plant proteases involved in this process remains unknown. Here, we establish an efficient identification system for the target proteases in Arabidopsis apoplastic fluid; the method employs native two-dimensional electrophoresis followed by an in-gel proteolytic assay using a fluorescence-quenching peptide substrate. We designed a substrate to specifically detect proteolytic activity at the C-terminus of the flg22 epitope in flagellin and identified two plant subtilases, SBT5.2 and SBT1.7, as specific proteases responsible for the C-terminal cleavage of flg22. In the apoplastic fluid of Arabidopsis mutant plants deficient in these two proteases, we observe a decrease in the C-terminal cleavage of the flg22 domain from flagellin, leading to a decrease in the efficiency of flg22 epitope liberation. Consequently, defensive reactive oxygen species (ROS) production is delayed in sbt5.2 sbt1.7 double-mutant leaf disks compared to wild type following flagellin exposure.

    DOI： 10.1038/s41467-024-48108-4

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  3. Peptide ligand-mediated trade-off between plant growth and stress response Reviewed International journal Open Access

    Mari Ogawa-Ohnishi, Tomohide Yamashita, Mitsuru Kakita, Takuya Nakayama, Yuri Ohkubo, Yoko Hayashi, Yasuko Yamashita, Taizo Nomura, Saki Noda, Hidefumi Shinohara, Yoshikatsu Matsubayashi

    Science   Vol. 378 ( 6616 ) page： 175 - 180   2022.10

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：American Association for the Advancement of Science (AAAS)  

    Deciding whether to grow or to divert energy to stress responses is a major physiological trade-off for plants surviving in fluctuating environments. We show that three leucine-rich repeat receptor kinases (LRR-RKs) act as direct ligand-perceiving receptors for PLANT PEPTIDE CONTAINING SULFATED TYROSINE (PSY)-family peptides and mediate switching between two opposing pathways. By contrast to known LRR-RKs, which activate signaling upon ligand binding, PSY receptors (PSYRs) activate the expression of various genes encoding stress response transcription factors upon depletion of the ligands. Loss of PSYRs results in defects in plant tolerance to both biotic and abiotic stresses. This ligand-deprivation–dependent activation system potentially enables plants to exert tuned regulation of stress responses in the tissues proximal to metabolically dysfunctional damaged sites where ligand production is impaired.

    DOI： 10.1126/science.abq5735

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  4. A type 2C protein phosphatase activates high-affinity nitrate uptake by dephosphorylating NRT2.1. Reviewed International journal Open Access

    Yuri Ohkubo, Keiko Kuwata, Yoshikatsu Matsubayashi

    Nature plants   Vol. 7 ( 3 ) page： 310 - +   2021.3

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    Authorship：Last author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

    The nitrate transporter NRT2.1, which plays a central role in high-affinity nitrate uptake in roots, is activated at the post-translational level in response to nitrogen (N) starvation1,2. However, the critical enzymes required for the post-translational activation of NRT2.1 remain to be identified. Here, we show that a type 2C protein phosphatase, designated CEPD-induced phosphatase (CEPH), activates high-affinity nitrate uptake by directly dephosphorylating Ser501 of NRT2.1, a residue that functions as a negative phospho-switch in Arabidopsis2. CEPH is predominantly expressed in epidermal and cortex cells in roots and is upregulated by N starvation via a CEPDL2/CEPD1/2-mediated long-distance signalling from shoots3,4. The loss of CEPH leads to marked decreases in high-affinity nitrate uptake, tissue nitrate content and plant biomass. Collectively, our results identify CEPH as a crucial enzyme in the N-starvation-dependent activation of NRT2.1 and provide molecular and mechanistic insights into how plants regulate high-affinity nitrate uptake at the post-translational level in response to the N environment.

    DOI： 10.1038/s41477-021-00870-9

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  5. Shoot-to-root mobile CEPD-like 2 integrates shoot nitrogen status to systemically regulate nitrate uptake in Arabidopsis. Reviewed International journal Open Access

    Ryosuke Ota, Yuri Ohkubo, Yasuko Yamashita, Mari Ogawa-Ohnishi, Yoshikatsu Matsubayashi

    Nature communications   Vol. 11 ( 1 ) page： 641 - 641   2020.1

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    Plants modulate the efficiency of root nitrogen (N) acquisition in response to shoot N demand. However, molecular components directly involved in this shoot-to-root communication remain to be identified. Here, we show that phloem-mobile CEPD-like 2 (CEPDL2) polypeptide is upregulated in the leaf vasculature in response to decreased shoot N status and, after translocation to the roots, promotes high-affinity uptake and root-to-shoot transport of nitrate. Loss of CEPDL2 leads to a reduction in shoot nitrate content and plant biomass. CEPDL2 contributes to N acquisition cooperatively with CEPD1 and CEPD2 which mediate root N status, and the complete loss of all three proteins severely impairs N homeostasis in plants. Reciprocal grafting analysis provides conclusive evidence that the shoot CEPDL2/CEPD1/2 genotype defines the high-affinity nitrate uptake activity in root. Our results indicate that plants integrate shoot N status and root N status in leaves and systemically regulate the efficiency of root N acquisition.

    DOI： 10.1038/s41467-020-14440-8

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  6. Shoot-to-root mobile polypeptides involved in systemic regulation of nitrogen acquisition Reviewed International journal Open Access

    Yuri Ohkubo, Mina Tanaka, Ryo Tabata, Mari Ogawa-Ohnishi, Yoshikatsu Matsubayashi

    NATURE PLANTS   Vol. 3 ( 4 ) page： 17029   2017.4

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    DOI： 10.1038/nplants.2017.29

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  7. A peptide hormone required for Casparian strip diffusion barrier formation in Arabidopsis roots Reviewed International journal Open Access

    Takuya Nakayama, Hidefumi Shinohara, Mina Tanaka, Koki Baba, Mari Ogawa-Ohnishi, Yoshikatsu Matsubayashi

    SCIENCE   Vol. 355 ( 6322 ) page： 284 - 286   2017.1

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    DOI： 10.1126/science.aai9057

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  8. Perception of root-derived peptides by shoot LRR-RKs mediates systemic N-demand signaling Reviewed

    Tabata Ryo, Sumida Kumiko, Yoshii Tomoaki, Ohyama Kentaro, Shinohara Hidefumi, Matsubayashi Yoshikatsu

    SCIENCE   Vol. 346 ( 6207 ) page： 343-346   2014.10

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    Authorship：Last author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1126/science.1257800

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  9. Secreted Peptide Signals Required for Maintenance of Root Stem Cell Niche in Arabidopsis Reviewed

    Matsuzaki Yo, Ogawa-Ohnishi Mari, Mori Ayaka, Matsubayashi Yoshikatsu

    SCIENCE   Vol. 329 ( 5995 ) page： 1065-1067   2010.8

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    Authorship：Last author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1126/science.1191132

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  10. Arabidopsis CLV3 peptide directly binds CLV1 ectodomain Reviewed

    Ogawa Mari, Shinohara Hidefumi, Sakagami Youji, Matsubayashi Yoshikatsu

    SCIENCE   Vol. 319 ( 5861 ) page： 294-294   2008.1

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    Authorship：Last author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1126/science.1150083

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  11. An LRR receptor kinase involved in the perception of a plant peptide hormone, phytosulfokine Reviewed

    Matsubayashi Y., Ogawa M., Morita A. and Sakagami Y.

    SCIENCE   Vol. 296   page： 1470-1472   2002.5

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  12. Antagonistic interactions between CLAVATA receptors shape maize ear development Reviewed International coauthorship

    Lindsay, PL; Xu, F; Liu, L; Boumpas, P; Reyes, A; Je, BI; Ogawa-Ohnishi, M; Man, J; Skopelitis, T; Matsubayashi, Y; Bartlett, M; Xu, SL; Jackson, D

    NEW PHYTOLOGIST     2026.1

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  13. Local peptide signalling induces stomatal closure under drought stress. Reviewed International journal Open Access

    Akie Shimotohno, Yoshikatsu Matsubayashi, Yujuan Du, Vajravijayan Senthilvadivel, Takamasa Suzuki, Hiroe Kato, Manish Kesherwani, Masaru Kono, Emi Mishiro-Sato, Keiko Kano, Mikiko Kojima, Yumiko Takebayashi, Hitoshi Sakakibara, Fumiyuki Soma, Florence Tama, Tsuyoshi Hirota, Keiko U Torii, Kazuko Yamaguchi-Shinozaki, Hiroo Fukuda

    Nature communications   Vol. 17 ( 1 ) page： 177 - 177   2025.12

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    In response to drought stress, land plants close their stomata to minimize transpiration. This action precedes a gradual accumulation of the stress hormone abscisic acid (ABA) that enhances plant drought tolerance. However, the molecular mechanisms that cause the time lag between the onset of stomatal closure and ABA accumulation and coordinate these two phases remain unexplained. Here, we found that Arabidopsis thaliana loss-of-function CLAVATA3/ENDOSPERM SURROUNDING REGION 5 (CLE5) mutants are less tolerant to drought. The CLE5 dodecapeptide (CLE5p) acts as a local signal to induce stomatal closure by binding to the LEUCINE-RICH REPEAT RECEPTOR-LIKE KINASE (LRR-RLK) receptor complex, BARELY ANY MERISTEM 1 (BAM1)-GUARD CELL HYDROGEN PEROXIDE-RESISTANT 1 (GHR1), in guard cells. The BAM1-GHR1-CLE5p module directly phosphorylates two SNF1-related protein kinases, OPEN STOMATA1 (SRK2E) and SRK2D, the central regulators of drought responses in plants, to regulate stomatal movement and drought-responsive gene expression without stimulating ABA biosynthesis or ROS accumulation. Our findings mark a critical step in understanding how plants promptly counteract environmental stresses. The CLEp-LRR-RLK signalling components are highly conserved across plant phyla, suggesting that peptide-mediated rapid stomatal closure is a widespread survival strategy and can be exploited to generate drought-resistant crops.

    DOI： 10.1038/s41467-025-66392-6

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  14. An Activity-Based Proteomics with Two- Dimensional Polyacrylamide Gel Electrophoresis (2D-PAGE) for Identifying Target Proteases in<i> Arabidopsis</i> Apoplastic Fluid Invited Reviewed International journal Open Access

    Sayaka Matsui, Yoshikatsu Matsubayashi

    BIO-PROTOCOL   Vol. 15 ( 5 ) page： e5226   2025.3

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.21769/BioProtoc.5226

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  15. Phototropin switches between <i>cis</i>‐ and <i>trans</i>‐autophosphorylation in light‐induced chloroplast relocation in <i>Marchantia polymorpha</i> Reviewed International journal

    Minoru Noguchi, Saki Noda, Yoshikatsu Matsubayashi, Yutaka Kodama

    The Plant Journal   Vol. 121 ( 1 ) page： e17183   2025.1

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Wiley  

    SUMMARY

    In the accumulation response, chloroplasts move toward weak blue light (BL) to maximize photosynthetic efficiency; in the avoidance response, they move away from strong BL to reduce photodamage. The BL receptor kinase phototropin (phot) mediates these chloroplast relocation responses, and the chloroplast relocation response requires phot kinase activity. Upon receiving BL, phot undergoes autophosphorylation; however, the molecular mechanisms that regulate chloroplast relocation through phot autophosphorylation remain unclear. In this study, we conducted biochemical experiments using phot in the liverwort Marchantia polymorpha and revealed that phot employs cis‐autophosphorylation under weak BL and both cis‐ and trans‐autophosphorylation under strong BL. Inhibiting trans‐autophosphorylation reduced phot autophosphorylation and suppressed the avoidance response, but not the accumulation response. These findings suggest that phot employs two modes of autophosphorylation to alternate between the accumulation and avoidance responses in plants.

    DOI： 10.1111/tpj.17183

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  16. IMA peptides regulate root nodulation and nitrogen homeostasis by providing iron according to internal nitrogen status. Reviewed International journal Open Access

    Momoyo Ito, Yuri Tajima, Mari Ogawa-Ohnishi, Hanna Nishida, Shohei Nosaki, Momona Noda, Naoyuki Sotta, Kensuke Kawade, Takehiro Kamiya, Toru Fujiwara, Yoshikatsu Matsubayashi, Takuya Suzaki

    Nature communications   Vol. 15 ( 1 ) page： 733 - 733   2024.1

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    Legumes control root nodule symbiosis (RNS) in response to environmental nitrogen availability. Despite the recent understanding of the molecular basis of external nitrate-mediated control of RNS, it remains mostly elusive how plants regulate physiological processes depending on internal nitrogen status. In addition, iron (Fe) acts as an essential element that enables symbiotic nitrogen fixation; however, the mechanism of Fe accumulation in nodules is poorly understood. Here, we focus on the transcriptome in response to internal nitrogen status during RNS in Lotus japonicus and identify that IRON MAN (IMA) peptide genes are expressed during symbiotic nitrogen fixation. We show that LjIMA1 and LjIMA2 expressed in the shoot and root play systemic and local roles in concentrating internal Fe to the nodule. Furthermore, IMA peptides have conserved roles in regulating nitrogen homeostasis by adjusting nitrogen-Fe balance in L. japonicus and Arabidopsis thaliana. These findings indicate that IMA-mediated Fe provision plays an essential role in regulating nitrogen-related physiological processes.

    DOI： 10.1038/s41467-024-44865-4

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  17. Evaluation of Direct Ligand-Receptor Interactions by Photoaffinity Labeling Invited Reviewed International journal

    Hidefumi Shinohara, Yoshikatsu Matsubayashi

    Methods in Molecular Biology   Vol. 2731   page： 231 - 240   2024

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    Language：English   Publishing type：Part of collection (book)   Publisher：Springer US  

    DOI： 10.1007/978-1-0716-3511-7_17

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  18. Extraction of Apoplastic Peptides for the Structural Elucidation of Mature Peptide Hormones in Arabidopsis. Invited Reviewed International journal

    Ogawa-Ohnishi M, Matsubayashi Y

    Methods in molecular biology (Clifton, N.J.)   Vol. 2731   page： 81 - 87   2024

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    DOI： 10.1007/978-1-0716-3511-7_6

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  19. Arabinogalactan protein polysaccharide chains are required for normal biogenesis of plasmodesmata. Reviewed International journal Open Access

    Ryoya Okawa, Yoko Hayashi, Yasuko Yamashita, Yoshikatsu Matsubayashi, Mari Ogawa-Ohnishi

    The Plant journal : for cell and molecular biology   Vol. 113 ( 3 ) page： 493 - 503   2023.2

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    Arabinogalactan proteins (AGPs) are a plant-specific family of extracellular proteoglycans characterized by large and complex galactose-rich polysaccharide chains. Functional elucidation of AGPs, however, has been hindered by the high degree of redundancy of AGP genes. To uncover as yet unexplored roles of AGPs in Arabidopsis, a mutant of Hyp O-galactosyltransferase (HPGT), a critical enzyme that catalyzes the common initial step of Hyp-linked arabinogalactan chain biosynthesis, was used. Here we show, using the hpgt1,2,3 triple mutant, that a reduction in functional AGPs leads to a stomatal patterning defect in which two or more stomata are clustered together. This defect is attributed to increased and dysregulated symplastic transport following changes in plasmodesmata structure, such that highly permeable complex branched plasmodesmata with cavities in branching parts increased in the mutant. We also found that the hpgt1,2,3 mutation causes a reduction of cellulose in the cell wall and accumulation of pectin, which controls cell wall porosity. Our results highlight the importance of AGPs in the correct biogenesis of plasmodesmata, possibly acting through the regulation of cell wall properties surrounding the plasmodesmata.

    DOI： 10.1111/tpj.16061

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  20. Genomic analysis of an ultrasmall freshwater green alga, Medakamo hakoo. Reviewed International journal Open Access

    Shoichi Kato, Osami Misumi, Shinichiro Maruyama, Hisayoshi Nozaki, Yayoi Tsujimoto-Inui, Mari Takusagawa, Shigekatsu Suzuki, Keiko Kuwata, Saki Noda, Nanami Ito, Yoji Okabe, Takuya Sakamoto, Fumi Yagisawa, Tomoko M Matsunaga, Yoshikatsu Matsubayashi, Haruyo Yamaguchi, Masanobu Kawachi, Haruko Kuroiwa, Tsuneyoshi Kuroiwa, Sachihiro Matsunaga

    Communications biology   Vol. 6 ( 1 ) page： 89 - 89   2023.1

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    Ultrasmall algae have attracted the attention of biologists investigating the basic mechanisms underlying living systems. Their potential as effective organisms for producing useful substances is also of interest in bioindustry. Although genomic information is indispensable for elucidating metabolism and promoting molecular breeding, many ultrasmall algae remain genetically uncharacterized. Here, we present the nuclear genome sequence of an ultrasmall green alga of freshwater habitats, Medakamo hakoo. Evolutionary analyses suggest that this species belongs to a new genus within the class Trebouxiophyceae. Sequencing analyses revealed that its genome, comprising 15.8 Mbp and 7629 genes, is among the smallest known genomes in the Viridiplantae. Its genome has relatively few genes associated with genetic information processing, basal transcription factors, and RNA transport. Comparative analyses revealed that 1263 orthogroups were shared among 15 ultrasmall algae from distinct phylogenetic lineages. The shared gene sets will enable identification of genes essential for algal metabolism and cellular functions.

    DOI： 10.1038/s42003-022-04367-9

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  21. Identification of receptors of plant peptide hormones by photoaffinity labeling

    Hidefumi Shinohara, Yoshikatsu Matsubayashi

    Yuki Gosei Kagaku Kyokaishi/Journal of Synthetic Organic Chemistry   Vol. 78 ( 7 ) page： 713 - 722   2020.7

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    Language：Japanese   Publishing type：Research paper (scientific journal)   Publisher：Society of Synthetic Organic Chemistry  

    DOI： 10.5059/yukigoseikyokaishi.78.713

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  22. Identification of Receptors of Plant Peptide Hormones by Photoaffinity Labeling

    Shinohara, H; Matsubayashi, Y

    JOURNAL OF SYNTHETIC ORGANIC CHEMISTRY JAPAN   Vol. 78 ( 7 ) page： 713 - 722   2020.7

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  23. Screening and identification of a non-peptide antagonist for the peptide hormone receptor in <i>Arabidopsis</i> Reviewed Open Access

    Shinohara H, Yasue N, Onuki T, Kondoh Y, Yoshida M, Matsubayashi Y

    Communications Biology   Vol. 2 ( 61 ) page： 61   2019.2

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    DOI： 10.1038/s42003-019-0307-8

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  24. PLENTY, a hydroxyproline O-arabinosyltransferase, negatively regulates root nodule symbiosis in Lotus japonicus. Reviewed International journal Open Access

    Emiko Yoro, Hanna Nishida, Mari Ogawa-Ohnishi, Chie Yoshida, Takuya Suzaki, Yoshikatsu Matsubayashi, Masayoshi Kawaguchi

    Journal of experimental botany   Vol. 70 ( 2 ) page： 507 - 517   2019.1

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Oxford University Press (OUP)  

    Legumes can survive in nitrogen-deficient environments by forming root-nodule symbioses with rhizobial bacteria; however, forming nodules consumes energy, and nodule numbers must thus be strictly controlled. Previous studies identified major negative regulators of nodulation in Lotus japonicus, including the small peptides CLAVATA3/ESR (CLE)-RELATED-ROOT SIGNAL1 (CLE-RS1), CLE-RS2, and CLE-RS3, and their putative major receptor HYPERNODULATION AND ABERRANT ROOT FORMATION1 (HAR1). CLE-RS2 is known to be expressed in rhizobia-inoculated roots, and is predicted to be post-translationally arabinosylated, a modification essential for its activity. Moreover, all three CLE-RSs suppress nodulation in a HAR1-dependent manner. Here, we identified PLENTY as a gene responsible for the previously isolated hypernodulation mutant plenty. PLENTY encoded a hydroxyproline O-arabinosyltransferase orthologous to ROOT DETERMINED NODULATION1 in Medicago truncatula. PLENTY was localized to the Golgi, and an in vitro analysis of the recombinant protein demonstrated its arabinosylation activity, indicating that CLE-RS1/2/3 may be substrates for PLENTY. The constitutive expression experiments showed that CLE-RS3 was the major candidate substrate for PLENTY, suggesting the substrate preference of PLENTY for individual CLE-RS peptides. Furthermore, a genetic analysis of the plenty har1 double mutant indicated the existence of another PLENTY-dependent and HAR1-independent pathway negatively regulating nodulation.

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  25. Lateral Inhibition by a Peptide Hormone-Receptor Cascade during Arabidopsis Lateral Root Founder Cell Formation. Reviewed International journal Open Access

    Koichi Toyokura, Tatsuaki Goh, Hidefumi Shinohara, Akinori Shinoda, Yuki Kondo, Yoshie Okamoto, Takeo Uehara, Koichi Fujimoto, Yoko Okushima, Yoshifumi Ikeyama, Keiji Nakajima, Tetsuro Mimura, Masao Tasaka, Yoshikatsu Matsubayashi, Hidehiro Fukaki

    Developmental cell   Vol. 48 ( 1 ) page： 64 - +   2019.1

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    In plants, the position of lateral roots (LRs) depends on initiation sites induced by auxin. The domain of high auxin response responsible for LR initiation stretches over several cells, but only a pair of pericycle cells (LR founder cells) will develop into LRs. In this work, we identified a signaling cascade controlling LR formation through lateral inhibition. It comprises a peptide hormone TARGET OF LBD SIXTEEN 2 (TOLS2), its receptor RLK7, and a downstream transcription factor PUCHI. TOLS2 is expressed at the LR founder cells and inhibits LR initiation. Time-lapse imaging of auxin-responsive DR5:LUCIFERASE reporter expression revealed that occasionally two pairs of LR founder cells are specified in close proximity even in wild-type and that one of them exists only transiently and disappears in an RLK7-dependent manner. We propose that the selection of LR founder cells by the peptide hormone-receptor cascade ensures proper LR spacing.

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  26. Pectin RG-I rhamnosyltransferases represent a novel plant-specific glycosyltransferase family. International journal

    Yuto Takenaka, Kohei Kato, Mari Ogawa-Ohnishi, Kana Tsuruhama, Hiroyuki Kajiura, Kenta Yagyu, Atsushi Takeda, Yoichi Takeda, Tadashi Kunieda, Ikuko Hara-Nishimura, Takeshi Kuroha, Kazuhiko Nishitani, Yoshikatsu Matsubayashi, Takeshi Ishimizu

    Nature plants   Vol. 4 ( 9 ) page： 669 - 676   2018.9

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    Pectin is one of the three key cell wall polysaccharides in land plants and consists of three major structural domains: homogalacturonan, rhamnogalacturonan I (RG-I) and RG-II. Although the glycosyltransferase required for the synthesis of the homogalacturonan and RG-II backbone was identified a decade ago, those for the synthesis of the RG-I backbone, which consists of the repeating disaccharide unit [→2)-α-L-Rha-(1 → 4)-α-D-GalUA-(1→], have remained unknown. Here, we report the identification and characterization of Arabidopsis RG-I:rhamnosyltransferases (RRTs), which transfer the rhamnose residue from UDP-β-L-rhamnose to RG-I oligosaccharides. RRT1, which is one of the four Arabidopsis RRTs, is a single-spanning transmembrane protein, localized to the Golgi apparatus. RRT1 was highly expressed during formation of the seed coat mucilage, which is a specialized cell wall with abundant RG-I. Loss-of-function mutation in RRT1 caused a reduction in the level of RG-I in the seed coat mucilage. The RRTs belong to a novel glycosyltransferase family, now designated GT106. This is a large plant-specific family, and glycosyltransferases in this family seem to have plant-specific roles, such as biosynthesis of plant cell wall polysaccharides.

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  27. Exploring peptide hormones in plants: identification of four peptide hormone-receptor pairs and two post-translational modification enzymes. Open Access

    Yoshikatsu Matsubayashi

    Proceedings of the Japan Academy. Series B, Physical and biological sciences   Vol. 94 ( 2 ) page： 59 - 74   2018.2

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    The identification of hormones and their receptors in multicellular organisms is one of the most exciting research areas and has lead to breakthroughs in understanding how their growth and development are regulated. In particular, peptide hormones offer advantages as cell-to-cell signals in that they can be synthesized rapidly and have the greatest diversity in their structure and function. Peptides often undergo post-translational modifications and proteolytic processing to generate small oligopeptide hormones. In plants, such small post-translationally modified peptides constitute the largest group of peptide hormones. We initially explored this type of peptide hormone using bioassay-guided fractionation and later by in silico gene screening coupled with biochemical peptide detection, which led to the identification of four types of novel peptide hormones in plants. We also identified specific receptors for these peptides and transferases required for their post-translational modification. This review summarizes how we discovered these peptide hormone-receptor pairs and post-translational modification enzymes, and how these molecules function in plant growth, development and environmental adaptation.

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  28. Analysis of PLETHORA Gradient Formation by Secreted Peptides During Root Development. International journal

    Hidefumi Shinohara, Yoshikatsu Matsubayashi

    Methods in molecular biology (Clifton, N.J.)   Vol. 1863   page： 155 - 164   2018

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    DOI： 10.1007/978-1-4939-8772-6_9

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  29. Cryptic bioactivity capacitated by synthetic hybrid plant peptides Reviewed International journal Open Access

    Yuki Hirakawa, Hidefumi Shinohara, Kai Welke, Stephan Irle, Yoshikatsu Matsubayashi, Keiko U. Torii, Naoyuki Uchida

    NATURE COMMUNICATIONS   Vol. 8   page： 14318   2017.2

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    DOI： 10.1038/ncomms14318

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  30. Expression of plant receptor kinases in tobacco By-2 cells Reviewed

    Hidefumi Shinohara, Yoshikatsu Matsubayashi

    Methods in Molecular Biology   Vol. 1621   page： 29 - 35   2017

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    DOI： 10.1007/978-1-4939-7063-6_3

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  31. Photoaffinity labeling of plant receptor kinases Reviewed

    Hidefumi Shinohara, Yoshikatsu Matsubayashi

    Methods in Molecular Biology   Vol. 1621   page： 59 - 68   2017

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    DOI： 10.1007/978-1-4939-7063-6_6

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  32. Long-distance peptide signaling essential for nutrient homeostasis in plants. Open Access

    Okamoto S, Tabata R, Matsubayashi Y

    Current opinion in plant biology   Vol. 34   page： 35-40   2016.12

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    DOI： 10.1016/j.pbi.2016.07.009

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  33. Loss of function at RAE2, a previously unidentified EPFL, is required for awnlessness in cultivated Asian rice. Open Access

    Bessho-Uehara K, Wang DR, Furuta T, Minami A, Nagai K, Gamuyao R, Asano K, Angeles-Shim RB, Shimizu Y, Ayano M, Komeda N, Doi K, Miura K, Toda Y, Kinoshita T, Okuda S, Higashiyama T, Nomoto M, Tada Y, Shinohara H, Matsubayashi Y, Greenberg A, Wu J, Yasui H, Yoshimura A, Mori H, McCouch SR, Ashikari M

    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA   Vol. 113 ( 32 ) page： 8969-74   2016.8

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    DOI： 10.1073/pnas.1604849113

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  34. Identification of three LRR-RKs involved in perception of root meristem growth factor in Arabidopsis. Reviewed Open Access

    Shinohara H, Mori A, Yasue N, Sumida K, Matsubayashi Y

    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA   Vol. 113 ( 14 ) page： 3897-902   2016.4

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    DOI： 10.1073/pnas.1522639113

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  35. A comprehensive strategy for identifying long-distance mobile peptides in xylem sap. Reviewed Open Access

    Okamoto S, Suzuki T, Kawaguchi M, Higashiyama T, Matsubayashi Y

    The Plant journal : for cell and molecular biology   Vol. 84 ( 3 ) page： 611-20   2015.11

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    DOI： 10.1111/tpj.13015

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  36. An Arabidopsis thaliana copper-sensitive mutant suggests a role of phytosulfokine in ethylene production Reviewed Open Access

    Wu Tao, Kamiya Takehiro, Yumoto Hiroko, Sotta Naoyuki, Katsushi Yamaguchi, Shigenobu Shuji, Matsubayashi Yoshikatsu, Fujiwara Toru

    JOURNAL OF EXPERIMENTAL BOTANY   Vol. 66 ( 13 ) page： 3657-3667   2015.7

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    DOI： 10.1093/jxb/erv105

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  37. A cascade of arabinosyltransferases controls shoot meristem size in tomato Reviewed

    Xu Cao, Liberatore Katie L., MacAlister Cora A., Huang Zejun, Chu Yi-Hsuan, Jiang Ke, Brooks Christopher, Ogawa-Ohnishi Mari, Xiong Guangyan, Pauly Markus, Van Eck Joyce, Matsubayashi Yoshikatsu, van der Knaap Esther, Lippman Zachary B.

    NATURE GENETICS   Vol. 47 ( 7 ) page： 784-+   2015.7

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    DOI： 10.1038/ng.3309

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  38. An Arabidopsis thaliana copper-sensitive mutant suggests a role of phytosulfokine in ethylene production. Reviewed Open Access

    Wu T, Kamiya T, Yumoto H, Sotta N, Katsushi Y, Shigenobu S, Matsubayashi Y, Fujiwara T

    Journal of experimental botany   Vol. 66 ( 13 ) page： 3657-67   2015.7

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  39. Reevaluation of the CLV3-receptor interaction in the shoot apical meristem: dissection of the CLV3 signaling pathway from a direct ligand-binding point of view Reviewed Open Access

    Shinohara Hidefumi, Matsubayashi Yoshikatsu

    PLANT JOURNAL   Vol. 82 ( 2 ) page： 328-336   2015.4

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    DOI： 10.1111/tpj.12817

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  40. Reevaluation of the CLV3-receptor interaction in the shoot apical meristem: dissection of the CLV3 signaling pathway from a direct ligand-binding point of view. Reviewed Open Access

    Shinohara H, Matsubayashi Y

    The Plant journal : for cell and molecular biology   Vol. 82 ( 2 ) page： 328-36   2015.4

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    DOI： 10.1111/tpj.12817

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  41. Identification of three potent hydroxyproline O-galactosyltransferases in Arabidopsis Reviewed Open Access

    Ogawa-Ohnishi Mari, Matsubayashi Yoshikatsu

    PLANT JOURNAL   Vol. 81 ( 5 ) page： 736-746   2015.3

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    DOI： 10.1111/tpj.12764

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  42. Identification of three potent hydroxyproline O-galactosyltransferases in Arabidopsis. Reviewed Open Access

    Ogawa-Ohnishi M, Matsubayashi Y

    The Plant journal : for cell and molecular biology   Vol. 81 ( 5 ) page： 736-46   2015.3

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  43. CLE6 expression recovers gibberellin deficiency to promote shoot growth in Arabidopsis Reviewed Open Access

    Bidadi Haniyeh, Matsuoka Keita, Sage-Ono Kimiyo, Fukushima Jun, Pitaksaringkarn Weerasak, Asahina Masashi, Yamaguchi Shinjiro, Sawa Shinichiro, Fukuda Hiroo, Matsubayashi Yoshikatsu, Ono Michiyuki, Satoh Shinobu

    PLANT JOURNAL   Vol. 78 ( 2 ) page： 241-252   2014.4

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    DOI： 10.1111/tpj.12475

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  44. Posttranslationally Modified Small-Peptide Signals in Plants Reviewed

    Matsubayashi Yoshikatsu

    ANNUAL REVIEW OF PLANT BIOLOGY, VOL 65   Vol. 65   page： 385-413   2014

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    DOI： 10.1146/annurev-arplant-050312-120122

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  45. Posttranslationally modified small-peptide signals in plants. Reviewed

    Matsubayashi Y

    Annual review of plant biology   Vol. 65   page： 385-413   2014

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    DOI： 10.1146/annurev-arplant-050312-120122

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  46. Identification of three hydroxyproline O-arabinosyltransferases in Arabidopsis thaliana Reviewed

    Ogawa-Ohnishi Mari, Matsushita Wataru, Matsubayashi Yoshikatsu

    NATURE CHEMICAL BIOLOGY   Vol. 9 ( 11 ) page： 726-+   2013.11

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    DOI： 10.1038/NCHEMBIO.1351

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  47. A Novel Pollen-Pistil Interaction Conferring High-Temperature Tolerance during Reproduction via CLE45 Signaling Reviewed Open Access

    Endo Satoshi MS, Shinohara Hidefumi, Matsubayashi Yoshikatsu, Fukuda Hiroo

    CURRENT BIOLOGY   Vol. 23 ( 17 ) page： 1670-1676   2013.9

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    DOI： 10.1016/j.cub.2013.06.060

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  48. Root-derived CLE glycopeptides control nodulation by direct binding to HAR1 receptor kinase Reviewed Open Access

    Okamoto Satoru, Shinohara Hidefumi, Mori Tomoko, Matsubayashi Yoshikatsu, Kawaguchi Masayoshi

    NATURE COMMUNICATIONS   Vol. 4   2013.8

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    DOI： 10.1038/ncomms3191

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  49. Chemical Synthesis of Arabidopsis CLV3 Glycopeptide Reveals the Impact of Hydroxyproline Arabinosylation on Peptide Conformation and Activity Reviewed Open Access

    Shinohara Hidefumi, Matsubayashi Yoshikatsu

    PLANT AND CELL PHYSIOLOGY   Vol. 54 ( 3 ) page： 369-374   2013.3

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    DOI： 10.1093/pcp/pcs174

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  50. Identification of three hydroxyproline O-arabinosyltransferases in Arabidopsis thaliana Reviewed

    Ogawa-Ohnishi M., Matsushita W., Matsubayashi Y.

    NATURE CHEMICAL BIOLOGY   Vol. 9   page： 726-730   2013.1

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    Hydroxyproline (Hyp) O-arabinosylation is a posttranslational modification that is prominent in extracellular glycoproteins in plants. Hyp O-arabinosylation is generally found in these glycoproteins in the form of linear oligoarabinoside chains and plays a key role in their function by contributing to conformational stability. However, Hyp O-arabinosyltransferase (HPAT), a key enzyme that catalyzes the transfer of the L-arabinose to the hydroxyl group of Hyp residues, has remained undiscovered. Here, we purified and identified Arabidopsis HPAT as a Golgi-localized transmembrane protein that is structurally similar to the glycosyltransferase GT8 family. Loss-of-function mutations in HPAT genes cause pleiotropic phenotypes that include enhanced hypocotyl elongation, defects in cell wall thickening, early flowering, early senescence, and impaired pollen tube growth. Our results indicate essential roles of Hyp O-arabinosylation in both vegetative and reproductive growth in plants.

    DOI： 10.1038/NCHEMBIO.1351

  51. Chemical synthesis of Arabidopsis CLV3 glycopeptide reveals the impact of hydroxyproline arabinosylation on peptide conformation and activity. Reviewed

    Shinohara H. and Matsubayashi Y.

    Plant Cell Physiol.   Vol. 54   page： 369-374   2013

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    Arabinosylation of hydroxyproline (Hyp) is a posttranslational modification often found in secreted peptide signals in plants. The physiological importance of this modification was highlighted by the finding that CLAVATA3 (CLV3), a key peptide signal for regulating the fate of stem cells in shoot apical meristem (SAM) in Arabidopsis, contains three L-arabinose residues linked via linear β-1,2-linkages. However, understanding the functions and properties of arabinosylated peptides have been hindered by difficulties in synthesizing the complex arabinose chain. Here we report the stereoselective total synthesis of β-1,2-linked tri-arabinosylated CLV3 peptide ([Ara3]CLV3). Chemically synthesized [Ara3]CLV3 restricted stem cell activity more effectively than did unmodified CLV3 peptide. Comparison of mono-, di- and tri-arabinosylated CLV3 glycopeptides revealed that the biological activity increased progressively as arabinose chain length increased. Thus, arabinose chain length of CLV3 is important for its biological activity. NMR spectroscopy and NOE-based structure calculations further revealed the structural impact of the arabinose chain on peptide conformation. The arabinose chain of [Ara3]CLV3 extends toward the C-terminal end of the peptide, and its non-reducing end is positioned proximal to the peptide backbone. Consequently, the arabinose chain causes distinct distortion in the C-terminal half of the peptide in a highly directional manner. The established synthetic route of [Ara3]CLV3 will greatly contribute to our understanding of the biology and biochemistry of arabinosylated peptide signals in plants.

  52. Biochemical mapping of a ligand-binding domain within Arabidopsis BAM1 reveals diversified ligand recognition mechanisms of plant LRR-RKs Reviewed Open Access

    Shinohara Hidefumi, Moriyama Yuji, Ohyama Kentaro, Matsubayashi Yoshikatsu

    PLANT JOURNAL   Vol. 70 ( 5 ) page： 845-854   2012.6

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    DOI： 10.1111/j.1365-313X.2012.04934.x

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  53. Recent progress in research on small post-translationally modified peptide signals in plants Reviewed Open Access

    Matsubayashi Yoshikatsu

    GENES TO CELLS   Vol. 17 ( 1 ) page： 1-10   2012.1

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    DOI： 10.1111/j.1365-2443.2011.01569.x

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  54. Biochemical mapping of a ligand-binding domain within Arabidopsis BAM1 reveals diversified ligand recognition mechanisms of plant LRR-RKs. Reviewed Open Access

    Shinohara H., Moriyama Y., Ohyama K., Matsubayashi Y.

    Plant J.   Vol. 70   page： 845-854   2012

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    Leucine-rich repeat receptor kinases (LRR-RKs) comprise the largest subfamily of the transmembrane receptor kinases in plants. In several LRR-RKs, a loop-out region called an island domain that intercepts the extracellular tandem LRRs at a position near the transmembrane domain constitutes the ligand-binding pocket, but the absence of the island domain in numerous LRR-RKs raises questions about which domain specifically recognizes the corresponding ligands in non-island domain-carrying LRR-RKs. Here, we determined by photoaffinity labeling followed by chemical and enzymatic digestion that BAM1, a CLV1/BAM family LRR-RK whose extracellular domain is comprised of 22 consecutive LRRs, directly interacts with the small peptide ligand CLE9 at the LRR6-8 region that is relatively distal from the transmembrane domain. Multiple sequence alignment and homology modeling revealed that the inner concave side of LRR6-8 of the CLV1/BAM family LRR-RKs is slightly deviatory from the LRR consensus. Supporting our findings, the clv1-4 mutant carries a missense mutation at the inner concave side of LRR6 of CLV1, and introduction of the corresponding mutation into BAM1 resulted in complete loss of ligand-binding activity. Our results indicate that ligand recognition mechanisms of plant LRR-RKs are more complex and diversified than anticipated.

    DOI： 10.1086/491575

  55. Post-Translational Modifications in Secreted Peptide Hormones in Plants Reviewed Open Access

    Matsubayashi Yoshikatsu

    PLANT AND CELL PHYSIOLOGY   Vol. 52 ( 1 ) page： 5-13   2011.1

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    DOI： 10.1093/pcp/pcq169

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  56. Arabinosylated glycopeptide hormones: new insights into CLAVATA3 structure Reviewed

    Shinohara Hidefumi, Matsubayashi Yoshikatsu

    CURRENT OPINION IN PLANT BIOLOGY   Vol. 13 ( 5 ) page： 515-519   2010.10

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    DOI： 10.1016/j.pbi.2010.05.008

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  57. Identification of tyrosylprotein sulfotransferase in Arabidopsis Reviewed Open Access

    Komori Ryota, Amano Yukari, Ogawa-Ohnishi Mari, Matsubayashi Yoshikatsu

    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA   Vol. 106 ( 35 ) page： 15067-15072   2009.9

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    DOI： 10.1073/pnas.0902801106

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  58. A glycopeptide regulating stem cell fate in Arabidopsis thaliana Reviewed

    Ohyama Kentaro, Shinohara Hidefumi, Ogawa-Ohnishi Mari, Matsubayashi Yoshikatsu

    NATURE CHEMICAL BIOLOGY   Vol. 5 ( 8 ) page： 578-580   2009.8

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    DOI： 10.1038/nchembio.182

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  59. Involvement of Phytosulfokine in the Attenuation of Stress Response during the Transdifferentiation of Zinnia Mesophyll Cells into Tracheary Elements Reviewed Open Access

    Motose Hiroyasu, Iwamoto Kuninori, Endo Satoshi, Demura Taku, Sakagami Youji, Matsubayashi Yoshikatsu, Moore Kevin L., Fukuda Hiroo

    PLANT PHYSIOLOGY   Vol. 150 ( 1 ) page： 437-447   2009.5

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    DOI： 10.1104/pp.109.135954

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  60. Non-cell-autonomous control of vascular stem cell fate by a CLE peptide/receptor system Reviewed Open Access

    Hirakawa Yuki, Shinohara Hidefumi, Kondo Yuki, Inoue Asuka, Nakanomyo Ikuko, Ogawa Mari, Sawa Shinichiro, Ohashi-Ito Kyoko, Matsubayashi Yoshikatsu, Fukuda Hiroo

    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA   Vol. 105 ( 39 ) page： 15208-15213   2008.9

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    DOI： 10.1073/pnas.0808444105

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  61. Identification of a biologically active, small, secreted peptide in Arabidopsis by in silico gene screening, followed by LC-MS-based structure analysis Reviewed Open Access

    Ohyama Kentaro, Ogawa Mari, Matsubayashi Yoshikatsu

    PLANT JOURNAL   Vol. 55 ( 1 ) page： 152-160   2008.7

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    DOI： 10.1111/j.1365-313X.2008.03464.x

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  62. Non-cell-autonomous control of vascular stem cell fate by a CLE peptide/receptor system. Reviewed Open Access

    Hirakawa Y., Shinohara H., Kondo Y., Inoue A., Nakanomyo I., Ogawa M., Sawa S., Ohashi-Ito K., Matsubayashi Y. and Fukuda H.

    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA   Vol. 105   page： 15208-15213   2008

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    DOI： 10.1073/pnas.0808444105

  63. Identification of a biologically active, small, secreted peptide in Arabidopsis by in silico gene screening, followed by LC-MS-based structure analysis. Reviewed Open Access

    Ohyama K, Ogawa M, Matsubayashi Y.

    Plant J.   Vol. 55   page： 152-160   2008

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    Peptidomics is a challenging field in which to create a link between genomic information and biological function through biochemical analysis of expressed peptides, including precise identification of post-translational modifications and proteolytic processing. We found that secreted peptides in Arabidopsis plants diffuse into the medium of whole-plant submerged cultures, and can be effectively identified by o-chlorophenol extraction followed by LC-MS analysis. Using this system, we first confirmed that a 12-amino-acid mature CLE44 peptide accumulated at a considerable level in the culture medium of transgenic plants overexpressing CLE44. Next, using an in silico approach, we identified a novel gene family encoding small secreted peptides that exhibit significant sequence similarity within the C-terminal short conserved domain. We determined that the mature peptide encoded by At1g47485, a member of this gene family, is a 15-amino-acid peptide containing two hydroxyproline residues derived from the conserved domain. This peptide, which we have named CEP1, is mainly expressed in the lateral root primordia and, when overexpressed or externally applied, significantly arrests root growth. CEP1 is a candidate for a novel peptide plant hormone.

    DOI： 10.1111/j.1365-313X.2008.03464.x

  64. A tyrosine-sulfated glycopeptide involved in cellular proliferation and expansion in Arabidopsis. Reviewed Open Access

    Amano Yukari, Tsubouchi Hiroko, Shinohara Hidefumi, Ogawa Mari, Matsubayashi Yoshikatsu

    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA   Vol. 104 ( 46 ) page： 18333-18338   2007.11

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    DOI： 10.1073/pnas.0706403104

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  65. Functional immobilization of plant receptor-like kinase onto microbeads towards receptor array construction and receptor-based ligand fishing Reviewed Open Access

    Shinohara Hidefumi, Matsubayashi Yoshikatsu

    PLANT JOURNAL   Vol. 52 ( 1 ) page： 175-184   2007.10

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    DOI： 10.1111/j.1365-313X.2007.03204.x

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  66. Identification of ligand binding site of phytosulfokine receptor by on-column photoaffinity labeling Reviewed Open Access

    Shinohara Hidefumi, Ogawa Mari, Sakagami Youji, Matsubayashi Yoshikatsu

    JOURNAL OF BIOLOGICAL CHEMISTRY   Vol. 282 ( 1 ) page： 124-131   2007.1

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1074/jbc.M604558200

    Open Access

    Web of Science

  67. Functional immobilization of plant receptor-like kinase onto microbeads toward receptor array construction and receptor-based ligand fishing Reviewed

    Shinohara H. and Matsubayashi Y.

    Plant J.   Vol. 52   page： 175-184   2007

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    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

    Despite the large number of receptor-like kinases (RLKs) in plants, few of them have known specific ligands. Ligand fishing is one of the most challenging post-genomic technologies. Here, we report a strategy for functional immobilization of plant RLKs on microbeads via covalent linkage. Due to the high density of immobilized RLK, ligand-receptor interaction can be visualized at high sensitivity using fluorescent-labeled ligands under the confocal laser scanning microscope. Moreover, using receptor-based affinity chromatography system with RLK microbeads, the ligand of the receptor was directly retrieved at high purity from complex natural samples. Our RLK microbeads and receptor-based ligand fishing approach is a feasible alternative to conventional forward genetics and bioassay-based biochemical purification for identification of novel ligand-receptor pairs in plants.

  68. Identification of ligand binding site of phytosulfokine receptor by on-column photoaffinity labeling. Reviewed Open Access

    Shinohara H, Ogawa M, Sakagami Y, and Matsubayashi Y.

    J. Biol. Chem.   Vol. 282   page： 124-131   2007

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    Language：English   Publishing type：Research paper (scientific journal)  

    Phytosulfokine (PSK), an endogenous 5-amino-acid secreted peptide in plants, affects cellular potential for growth via binding to PSKR1, a member of the leucine-rich repeat receptor kinase (LRR-RK) family. PSK interacts with PSKR1 in a highly specific manner with a nanomolar dissociation constant. However, it is not known which residues in the PSKR1 extracellular domain constitute the ligand-binding pocket. Here, we identified the PSK-binding domain of carrot PSKR1 (DcPSKR1) by photoaffinity labeling. We cross-linked the photoactivatable PSK analog [125I]Ne-[(4-azidosalicyl)Lys5]PSK with DcPSKR1 using UV irradiation, and mapped the cross-linked region using chemical and enzymatic fragmentation. We also established a novel メon-column photoaffinity labelingモ methodology that allows repeated incorporation of the photoaffinity label to increase the efficiency of the photoaffinity cross-linking reactions. We purified a labeled DcPSKR1 tryptic fragment using anti-PSK antibodies, and identified a peptide fragment that corresponds to the 15-amino-acid Glu503-Lys517 region of DcPSKR1 by MALDI-TOF MS. Deletion of Glu503-Lys517 completely abolishes the ligand-binding activity of DcPSKR1. This region is in the island domain flanked by extracellular LRRs, indicating that this domain forms a ligand-binding pocket that directly interacts with PSK.

    DOI： 10.1074/jbc.M604558200

    Open Access

  69. Functional analysis of phytosulfokine receptor homologs in Arabidopsis Reviewed

    Tsubouchi Hiroko, Shinohara Hidefumi, Ogawa Mari, Sakagami Youji, Matsubayashi Yoshikatsu

    PLANT AND CELL PHYSIOLOGY   Vol. 48   page： S26-S26   2007

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    Language：English   Publishing type：Research paper (scientific journal)  

    Web of Science

  70. Identification of ligand binding site of phytosulfokine by on-column photoaffinity labeling Reviewed

    Shmohara Hidefumi, Ogawa Mari, Sakagami Youji, Matsubayashi Yoshikatsu

    PLANT AND CELL PHYSIOLOGY   Vol. 48   page： S26-S26   2007

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    Language：English   Publishing type：Research paper (scientific journal)  

    Web of Science

  71. Disruption and overexpression of Arabidopsis phytosulfokine receptor gene affects cellular longevity and potential for growth Reviewed Open Access

    Matsubayashi Yoshikatsu, Ogawa Mari, Kihara Hitomi, Niwa Masaaki, Sakagami Youji

    PLANT PHYSIOLOGY   Vol. 142 ( 1 ) page： 45-53   2006.9

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1104/pp.106.081109

    Web of Science

  72. Peptide hormones in plants Invited

    Matsubayashi Y. and Sakagami Y.

    Annu. Rev. Plant Biol.   Vol. 57   page： 649-674   2006

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    Authorship：Lead author   Language：English  

    In recent years, numerous biochemical and genetic studies have demonstrated that peptide signaling plays a greater than anticipated role in various aspects of plant growth and development. A substantial proportion of these peptides are secretory and act as local signals mediating cell-to-cell communication. Specific receptors for several peptides were identified as being membrane-localized receptor kinases, the largest family of receptor-like molecules in plants. These findings illustrate the importance of peptide signaling in the regulation of plant growth, functions that were previously ascribed to the combined action of small lipophilic compounds referred to as メtraditional plant hormones.モ Here, we outline recent advances in the current understanding of biologically active peptides in plants, currently regarded as a new class of plant hormones.

  73. Disruption and overexpression of Arabidopsis phytosulfokine receptor gene affects cellular longevity and potential for growth. Reviewed Open Access

    Matsubayashi, Y., Ogawa, M., Kihara, H., Niwa, M., and Sakagami, Y.

    Plant Physiol.   Vol. 142   page： 45-53   2006

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    Language：English   Publishing type：Research paper (scientific journal)  

    Phytosulfokine (PSK), a 5-amino-acid sulfated peptide that has been identified in conditioned medium of plant cell cultures, promotes cellular growth in vitro via binding to the membrane-localized PSK receptor. Here, we report that loss-of-function and gain-of-function mutations of the Arabidopsis PSK receptor (AtPSKR1) alter cellular longevity and potential for growth without interfering with basic morphogenesis of plants. Although mutant pskr1-1 plants exhibit morphologically normal growth until 3 weeks after germination, individual pskr1-1 cells gradually lose their potential to form calluses as tissues mature. Shortly after a pskr1-1 callus forms, it loses potential for growth, resulting in formation of a smaller callus than the wild-type. Leaves of pskr1-1 plants exhibit premature senescence after bolting. Leaves of AtPSKR1ox plants exhibit greater longevity and significantly greater potential for callus formation than leaves of wild-type plants, irrespective of their age. Calluses derived from AtPSKR1ox plants maintain their potential for growth longer than wild-type calluses. Combined with the present finding that PSK precursor genes are more strongly expressed in mature plant parts than in immature plant parts, the available evidence indicates that PSK signaling affects cellular longevity and potential for growth, and thereby exerts a pleiotropic effect on cultured tissue in response to environmental hormonal conditions.

    DOI： 10.1104/pp.106.081109

  74. Identification and functional characterization of phytosulfokine receptor using a ligand-based approach Invited Reviewed

    Matsubayashi Y., Shinohara H. and Ogawa M.

    Chem. Rec.   Vol. 6   page： 356-364   2006

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    Authorship：Lead author   Language：English  

    DOI： 10.1002/tcr.20090

  75. Peptide hormones in plants Reviewed

    Matsubayashi Yoshikatsu, Sakagami Youji

    ANNUAL REVIEW OF PLANT BIOLOGY   Vol. 57   page： 649-674   2006

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1146/annurev.arplant.56.032604.144204

    Web of Science

  76. Identification and functional characterization of phytosulfokine receptor using a ligand-based approach Reviewed

    Matsubayashi Yoshikatsu, Shinohara Hidefumi, Ogawa Mari

    CHEMICAL RECORD   Vol. 6 ( 6 ) page： 356-364   2006

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1002/tcr.20090

    Web of Science

  77. Phytosulfokine Reviewed

    Matsubayashi Yoshikatsu, Sakagami Youji

    HANDBOOK OF BIOLOGICALLY ACTIVE PEPTIDES     page： 29-32   2006

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    Language：English   Publishing type：Research paper (scientific journal)  

    Web of Science

  78. Ion-selective enrichment of tyrosine-sulfated peptides from complex protein digests. Reviewed

    Amano Y., Shinohara H., Sakagami Y. and Matsubayashi Y.

    Anal. Biochem.   Vol. 346   page： 124-131   2005

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    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

    We have developed a novel procedure for concentrating sulfated peptides, as a front end to mass spectrometric analysis, based on ion-selective interaction of sulfate ions with anion exchangers. Ions with a higher charge and smaller solvated ion radius, such as sulfate ions, have higher retention in an ion exchanger, due to their greater degree of coulombic interactions. We tested the effectiveness of this approach for enrichment and identification of sulfated peptides, using a tryptic digest of bovine serum albumin spiked with model sulfated peptide (molar ratio, 20:1), and using a tryptic digest of bovine fibrinogen. Sulfated peptides are identified by mass spectrometry, in which both the molecular ion and its specific fragment ion produced by facile loss of SO3 are detected. In both experiments, sulfated peptides were strongly retained on the anion exchanger, and were eluted by higher concentrations of competing ion with minimum contamination of non-sulfated peptides. Using this procedure, we determined that the 13-amino-acid C-terminal peptide of the minor gユ chain of bovine fibrinogen contains sulfated tyrosine.

  79. Further analogues of plant peptide hormone phytosulfokine-alpha (PSK-alpha) and their biological evaluation Reviewed

    Bahyrycz A., Matsubayashi Y., Ogawa M., Sakagami Y. and Konopinska D.

    J. Pept Sci.   Vol. 11   page： 589-592   2005

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    Language：English   Publishing type：Research paper (scientific journal)  

  80. Two stimulatory effects of the peptidyl growth factor phytosulfokine during somatic embryogenesis in Japanese larch (Larix leptolepis Gordon). Reviewed

    Umehara M., Ogita S., Sasamoto H., Eun CH., Matsubayashi Y., Sakagami Y. and Kamada H.

    Plant Sci.   Vol. 169   page： 901-907   2005

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.plantsci.2005.06.008

  81. Plant peptide hormone phytosulfokine (PSK-alpha) and its analogues Reviewed

    Bahyrycz Agata, Matsubayashi Yoshikatsu, Ogawa Mari, Sakagami Youji, Konopinska Danuta

    Peptides 2004, Proceedings     page： 784-785   2005

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    Language：English   Publishing type：Research paper (scientific journal)  

    Web of Science

  82. Chemical nursing: phytosulfokine improves genetic transformation efficiency by promoting the proliferation of surviving cells on selective media. Reviewed

    Matsubayashi Y., Goto T. and Sakagami Y.

    Plant Cell Rep.   Vol. 23   page： 155-158   2004

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1007/s00299-004-0816-9

  83. Plant peptide hormone phytosulfokine (PSK-alpha): synthesis of new analogues and their biological evaluation Reviewed

    Bahyrycz A., Matsubayashi Y., Ogawa M., Sakagami Y. and Konopinska D.

    J. Pept Sci.   Vol. 10   page： 462-469   2004

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    Language：English   Publishing type：Research paper (scientific journal)  

  84. Phytosulfokine stimulates somatic embryogenesis in Cryptomeria japonica. Reviewed Open Access

    Igasaki T., Akashi N., Ujino-Ihara T., Matsubayashi Y., Sakagami Y. and Shinohara K.

    Plant Cell Physiol.   Vol. 44   page： 1412-1416   2003

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1093/pcp/pcg161

  85. Ligand-receptor pairs in plant peptide signaling. Invited Reviewed

    Matsubayashi Y.

    J. Cell Sci.   Vol. 116   page： 3863-3870   2003

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    Authorship：Lead author   Language：English  

    DOI： 10.1242/jcs.00733

  86. Phytosulfokaine-α,a peptidyl plant grouth factor,stimulates cell cycle progression in carrot nonembryogenic cells.

    Plant Biotechnol   Vol. 20   page： 195-205   2003

  87. 植物細胞の増殖を制御するペプチド

    松林嘉克

        2001.7

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    Language：Japanese  

    "Peptide Newsletter Japan, 41, 5-7"

  88. ペプチド性植物細胞増殖因子に関する研究(総説). Open Access

    松林嘉克

    日本農芸化学会誌   Vol. 75   page： 1275-1281   2001.1

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  89. Peptide signals and their receptors in higher plant.

    "Matsubayashi Y., Yang H. and Sakagami Y."

    Trends in Plant Science   Vol. 6   page： 573-577   2001.1

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    Language：English  

  90. Diversity of arabidopsis genes encoding precursors for phytosulfokine,a peptide growth factor. Reviewed

    Plant Physiol.   Vol. 127   page： 842-851   2001

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    Language：English   Publishing type：Research paper (scientific journal)  

  91. Peptide signals and their receptors in higher plants

    Trends in Plant Science   Vol. 6   page： 573-577   2001

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  92. Peptide growth factor phytosulfokine-a contributes to pollen population effect.

    "Chen Y.F., Matsubayashi Y. and Sakagami Y."

    Planta   Vol. 211   page： 752-755   2000.1

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    Language：English   Publishing type：Research paper (scientific journal)  

  93. "Phytosulfokine-α, a peptide growth factor found in higher plants: Its structure, functions, precursor and receptors."

    "Yang H., Matsubayashi Y., Hanai H. and Sakagami Y."

    Plant and Cell Physiology   Vol. 41   page： 825-830   2000.1

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    Language：English  

  94. Existence of a plant tyrosylprotein sulfotransferase: novel plant enzyme catalyzing tyrosine O-sulfation of preprophytosulfokine variants in vitro.

    "Hanai H., Nakayama D., Yang H., Matsubayashi Y., Hirota Y. and Sakagami Y."

    FEBS Letters   Vol. 470   page： 97-101   2000.1

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    Language：English   Publishing type：Research paper (scientific journal)  

  95. "Rapid and efficient Agrobacterium infection-mediated transient gene expression in rice Oc cells and its application for expression and suppression of OsPSK cDNA for a precursor to phytosulfokine-a, a peptide plant growth factor."

    "Yang H., Morita A., Matsubayashi Y., Nakamura K. and Sakagami Y."

    Plant and Cell Physiology   Vol. 41   page： 811-816   2000.1

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    Language：English   Publishing type：Research paper (scientific journal)  

  96. "Molecular cloning and characterization of OsPSK, a gene encoding a precursor for phytosulfokine-α, required for rice cell proliferation."

    "Yang H., Matsubayashi Y., Hanai H., Nakamura K. and Sakagami Y."

    Plant Molecular Biology   Vol. 44   page： 635-647   2000.1

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    Language：English   Publishing type：Research paper (scientific journal)  

  97. 120- and 160-kDa receptors for endogenous mitogenic peptide, phytosulfokine-alpha, in rice plasma membranes. Reviewed

    J. Biol. Chem.   Vol. 275   page： 15520-15525   2000

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    Language：English   Publishing type：Research paper (scientific journal)  

  98. A secreted peptide growth factor, phytosulfokine,acting as a stimulatory factor of carrot somatic embryo formation. Reviewed Open Access

    Plant Cell Physiol.   Vol. 41   page： 27-32   2000

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1088/0004-637X/749/2/173

  99. "Promotive effects of the peptidyl plant growth factor, phytosulfokine-a, on the growth and chlorophyll content of Arabidopsis seedlings under high night-time temperature conditions."

    "Yamakawa S., Matsubayashi Y. Sakagami Y., Kamada H. and Satoh S."

    "Bioscience, Biotechnology and Biochemistry"   Vol. 63   page： 2240-2243   1999.1

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    Language：English   Publishing type：Research paper (scientific journal)  

  100. "Stimulatory effects of a novel peptidyl plant growth factor, phytosulfokine-a, on the adventitious bud formation from callus of Antirrhium majus."

    "Yang G., Shen T., Kobayashi Y., Matsubayashi Y., Sakagami Y. and Kamada H."

    Plant Biotechnology   Vol. 16   page： 231-234   1999.1

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    Language：English   Publishing type：Research paper (scientific journal)  

  101. Oryza sativa PSK gene encodes a precursor ofphytosulfokine-alpha, a sulfated peptide growthfactor found in plants. Reviewed

    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA   Vol. 96   page： 13560-13565   1999

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    Language：English   Publishing type：Research paper (scientific journal)  

  102. Characterization of specific binding sites for a mitogenic sulfated peptide, phytosulfokine-alpha, in the plasma membrane fraction derived from Oryza sativa L. Reviewed

    Eur. J. Biochem.   Vol. 262   page： 666-671   1999

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1007/s00441-003-0732-2

  103. Preparation and characterization of fully active biotinylated analogs of phytosulfokine-alpha. Reviewed

    Biosci. Biotechnol. Biochem.   Vol. 63   page： 1847-1849   1999

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    Language：English   Publishing type：Research paper (scientific journal)  

  104. The endogenous sulfated pentapeptide, phytosulfokine-alpha, stimulates tracheary element differentiation of isolated mesophyll cells of Zinnia elegans. Reviewed

    Plant Physiol.   Vol. 120   page： 1043-1048   1999

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    Language：English   Publishing type：Research paper (scientific journal)  

  105. Physiological relationships between auxin, cytokinin, and a peptide growth factor, phytosulfokine-α, in stimulation of asparagus cell proliferation.

    Planta   Vol. 207   page： 559-565   1999

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    Language：English  

    DOI： 10.1007/s004250050518

  106. Phytosulfokine-alpha, a peptidyl plant growth factor, stimulates somatic embryogenesis in carrot. Reviewed

    J. Exp. Bot.   Vol. 50   page： 1123-1128   1999

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    Language：English   Publishing type：Research paper (scientific journal)  

  107. "The promotive effects of a peptidyl plant growth factor, phytosulfokine-α,on the formation of adventitious roots and expression of a gene for a root-specific cystain in cucumber hypocotyls."

    "Yamakawa S., Sakuta C., Matsubayshi Y., Sakagami Y., Kamada H. and Satoh S."

    Journal of Plant Research   Vol. 111   page： 453-458   1998.1

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    Language：English   Publishing type：Research paper (scientific journal)  

  108. Differential induction by methyl jasmonate of genes encoding ornithine decarboxylase and other enzymes involved in nicotine biosynthesis in tobacco cell cultures.

    "Imanishi S., Hashizume K., Nakakita M., Kojima H., Matsubayashi Y., Hashimoto T., Sakagami Y., Yamada Y. and Nakamura K."

    Plant Molecular Biology   Vol. 38   page： 1101-1111   1998.1

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1023/A:1006058700949

  109. Promotion by a peptidyl growth factor, phytosulfokine-α, of chlorophyll formation in etiolated cotyledon of cucumber

    Yamakawa S., Matsubayashi Y., Sakagami Y., Kamada H. and Satoh S.

    Biosci. Biotech. Biochem.   Vol. 62   page： 2441-2443   1998

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    Language：English   Publishing type：Research paper (scientific journal)  

  110. Effect of medium ammonium-nitrate ratio on competence for cell division induced by phytosulfokine-alpha. Reviewed

    Plant Cell Rep.   Vol. 17   page： 368-372   1998

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    Language：English   Publishing type：Research paper (scientific journal)  

  111. 植物細胞の増殖を誘導するペプチド性因子.

    "松林嘉克,坂神洋次"

    化学と生物   Vol. 35   page： 6-8   1997.1

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    Language：Japanese  

  112. 植物細胞増殖因子(総説). Open Access

    "松林嘉克,坂神洋次"

    植物の化学調節   Vol. 32   page： 160-171   1997.1

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    Language：Japanese  

    DOI： 10.18978/jscrpi.32.2_160

    Open Access

  113. Phytosulfokine-alpha, a sulfated pentapeptide, stimulates the proliferation of rice cells by means of specific high-and low-affinity binding sites. Reviewed

    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA   Vol. 94   page： 13357-13362   1997

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1073/pnas.94.24.13357

  114. 植物細胞増殖因子(ミニレビュー). Open Access

    "松林嘉克,坂神洋次"

    日本農芸化学会誌   Vol. 70   page： 588-590   1996.1

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  115. Phytosulfokine, sulfated peptides that induce the proliferation of single cells of Asparagus officinalis L. Reviewed

    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA   Vol. 93   page： 7623-7627   1996

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    Authorship：Lead author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

  116. Active fragments and analogs of the plaant growth factor, phytosulfokine : Structure-activity relationships. Reviewed

    Biochem. Biophys. Res. Commun.   Vol. 225   page： 209-214   1996

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    Language：English   Publishing type：Research paper (scientific journal)  

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To the head of Papers.▲

Books 7

  1. 植物の行動生態学

    松林嘉克（ Role： Joint author ,  第7章 長距離移行ペプチドを介した植物の変動窒素環境への適応）

    種生物学会  2023.1 

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    Language：Japanese Book type：Scholarly book

  2. Evaluation of direct ligand-receptor interactions by photoaffinity labeling Reviewed

    H Shinohara, Y Matsubayashi（ Role： Joint author）

    Plant Peptide Hormones and Growth Factors  2023 

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    Responsible for pages：231-240   Language：English Book type：Scholarly book

  3. Extraction of apoplastic peptides for the structural elucidation of mature peptide hormones in Arabidopsis Reviewed

    M Ogawa-Ohnishi, Y Matsubayashi（ Role： Joint author）

    Plant Peptide Hormones and Growth Factors  2023 

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    Responsible for pages：81-87   Language：English Book type：Scholarly book

  4. 植物の低窒素環境における生存戦略

    大久保祐里，松林嘉克，木羽隆敏（ Role： Joint author）

    化学と生物/日本農芸化学会  2022.7 

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    Responsible for pages：352-359   Language：Japanese Book type：Scholarly book

  5. 植物の窒素吸収を制御する長距離情報伝達 −葉と根のコミュニケーションによる需要と供給の調節機構−

    大久保祐里，松林嘉克（ Role： Joint author）

    情報処理/情報処理学会  2021.11 

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    Responsible for pages：e10-e12   Language：Japanese Book type：Scholarly book

  6. 植物の窒素栄養吸収制御の巧みなしくみ

    大久保祐里，松林嘉克（ Role： Joint author）

    生化学/日本生化学会  2021.6 

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    Responsible for pages：400-403   Language：Japanese Book type：Scholarly book

  7. 窒素取り込みを制御する長距離移行ペプチド群

    大久保祐里，松林嘉克（ Role： Joint author）

    アグリバイオ/北隆館  2021.5 

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    Language：Japanese Book type：Scholarly book

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To the head of Books.▲

MISC 8

  1. 植物の低栄養耐性-1 植物の低窒素環境における生存戦略 窒素不足に適応する巧妙な仕組み

    大久保祐里, 松林嘉克, 木羽隆敏

    化学と生物   Vol. 60 ( 7 )   2022

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  2. [植物と情報処理]〈カテゴリ1 植物の情報処理機能〉1.4 植物の窒素吸収を制御する長距離情報伝達-葉と根のコミュニケーションによる需要と供給の調節機構-

    大久保祐里, 松林嘉克

    情報処理   Vol. 62 ( 12 )   2021

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  3. Long-distance mobile peptides regulating nitrogen acquisition in roots

    大久保祐里, 松林嘉克

    アグリバイオ   Vol. 5 ( 5 )   2021

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  4. An ingenious mechanism of regulating nitrogen acquisition in plants.

    大久保祐里, 松林嘉克

    生化学   Vol. 93 ( 3 )   2021

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  5. 植物の生きる知恵 2 根と葉のコミュニケーションを介した植物の巧妙な窒素取り込み制御

    大久保祐里, 松林嘉克

    生物の科学 遺伝   Vol. 74 ( 5 )   2020

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  6. P4-2-2 局所的な鉄欠乏に応答した植物の長距離シグナル制御機構（4-2 植物の微量栄養素，2019年静岡大会）

    田畑 亮, 生田 久美子, 田村 花, 神谷 岳洋, 田野井 慶太朗, 松林 嘉克, 榊原 均

    日本土壌肥料学会講演要旨集   Vol. 65 ( 0 ) page： 60 - 60   2019

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    Language：Japanese   Publisher：一般社団法人 日本土壌肥料学会  

    DOI： 10.20710/dohikouen.65.0_60_3

    CiNii Research

    researchmap

  7. 植物の生長調節 カスパリー線の形成に必要なチロシン硫酸化ペプチドホルモン

    藤田智史, 篠原秀文, 松林嘉克

    植物の生長調節   Vol. 53 ( 2 ) page： 102‐108   2018.12

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  8. 植物の生長調節 根の窒素吸収を制御する長距離移行ペプチド群

    田畑亮, 大久保祐里, 松林嘉克

    植物の生長調節   Vol. 53 ( 2 )   2018

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To the head of MISC.▲

Presentations 25

  1. Plant adaptation to fluctuating nitrogen environment by long-range mobile peptide signals Invited International conference

    The 7th International Conference on Plant Vascular Biology  2025.7.10 

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    Event date： 2025.7

    Language：English   Presentation type：Oral presentation (invited, special)  

    Venue：Osaka, Japan  

  2. Plant adaptation to fluctuating nitrogen environment by long-range mobile peptide signals Invited International conference

    The 24th International Conference on Plant Growth Substances  2025.6.30 

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    Event date： 2025.6 - 2025.7

    Language：English   Presentation type：Oral presentation (invited, special)  

    Venue：Colorado, USA  

  3. 植物生理学会賞受賞講演：新規ペプチドホルモンの同定を基軸とした植物成長・環境適応機構の解明 Invited

    松林嘉克

    第67回日本植物生理学会年会  2025.3.14  日本植物生理学会

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    Event date： 2025.3

    Language：Japanese   Presentation type：Symposium, workshop panel (nominated)  

  4. 葉と根を行き交うペプチドシグナルによる変動する窒素環境への適応 Invited

    大久保祐里，松林嘉克

    第65回日本植物生理学会年会  2024.3.17  日本植物生理学会

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    Event date： 2024.3

    Language：Japanese   Presentation type：Symposium, workshop panel (nominated)  

    Venue：神戸  

  5. 細菌由来の免疫誘導ペプチドflg22のC末端を切り出す植物プロテアーゼの同定

    松井 彩，松林嘉克

    第65回日本植物生理学会年会  2024.3.17  日本植物生理学会

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    Event date： 2024.3

    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：神戸  

  6. Plant adaptation to fluctuating nitrogen environments by long-range mobile peptide signals Invited International conference

    Nitrogen2023  2023.11.8 

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    Event date： 2023.11

    Language：English   Presentation type：Oral presentation (keynote)  

  7. Shoot-derived polypeptides regulate nitrate uptake in the roots under fluctuating nitrogen environments International conference

    Nitrogen2023  2023.11.9 

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    Event date： 2023.11

    Language：English   Presentation type：Oral presentation (general)  

  8. Plant adaptation to fluctuating nitrogen environments by long-range mobile peptide signals Invited International conference

    11th Plant Peptides and Receptors Meeting  2023.10.3 

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    Event date： 2023.10

    Language：English   Presentation type：Oral presentation (keynote)  

  9. 植物の硝酸吸収活性を制御するリン酸化／脱リン酸化酵素の解析

    大久保祐里，松林嘉克

    日本植物学会第87回大会  2023.9.7  日本植物学会

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    Event date： 2023.9

    Language：Japanese   Presentation type：Poster presentation  

    Venue：札幌  

  10. 転写因子TGAと長距離移行ペプチドによる硝酸吸収の統御メカニズム

    大久保祐里，松林嘉克

    植物の栄養研究会 第8回研究交流会  2023.9.5  植物の栄養研究会 第8回研究交流会

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    Event date： 2023.9

    Language：Japanese   Presentation type：Poster presentation  

    Venue：札幌  

  11. Exploring peptide hormones in plants: Identification of five peptide hormone-receptor pairs and two post-translational modification enzymes Invited International conference

    The 24th International Conference on Plant Growth Substances  2023.7.4 

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    Event date： 2023.7

    Language：English   Presentation type：Oral presentation (invited, special)  

    Venue：Gyeongju, Korea  

  12. Peptide signal-mediated adaptation to spatially and temporally flucuating environments in plants Invited International conference

    The 33rd International Conference on Arabidopsis Research  2023.6.9 

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    Event date： 2023.6

    Language：English   Presentation type：Oral presentation (keynote)  

  13. Regulation of high-affinity nitrate uptake activity by NRT2.1 phosphorylation Invited International conference

    The 33rd International Conference on Arabidopsis Research  2023.6.9 

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    Event date： 2023.6

    Language：English   Presentation type：Poster presentation  

  14. AGP polysaccharide chains are required for normal biogenesis of plasmodesmata Invited International conference

    The 33rd International Conference on Arabidopsis Research  2023.6.9 

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    Event date： 2023.6

    Language：English   Presentation type：Poster presentation  

  15. 植物のストレス応答と成長のトレードオフを制御するペプチドホルモン Invited

    大西（小川）真理，山下智偉，垣田満，中山卓也，大久保祐里，林陽子，山下恭子，野村泰三，野田沙希，篠原秀文，松林嘉克

    第64回日本植物生理学会年会  2023.3  日本植物生理学会

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    Event date： 2023.3

    Language：Japanese   Presentation type：Symposium, workshop panel (nominated)  

    Venue：京都  

  16. Peptide ligand-mediated trade-off between plant growth and stress response Invited International conference

    松林嘉克

    38th IPSR International Symposium  2023.2.27  岡山大学資源植物科学研究所

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    Event date： 2023.2

    Language：English   Presentation type：Symposium, workshop panel (nominated)  

    Venue：岡山   Country：Japan  

  17. 定量リン酸化プロテオミクスを用いた植物プロテインフォスファターゼの基質探索 Invited

    松林嘉克，大久保祐里

    第95回日本生化学会大会シンポジウム  2022.11.9  日本生化学会

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    Event date： 2022.11

    Language：Japanese   Presentation type：Symposium, workshop panel (nominated)  

    Venue：名古屋  

  18. 葉と根を行き交うペプチド群による植物の窒素吸収制御 Invited

    大久保祐里，松林嘉克

    第95回日本生化学会大会シンポジウム  2022.11.9  日本生化学会

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    Event date： 2022.11

    Language：Japanese   Presentation type：Symposium, workshop panel (nominated)  

    Venue：名古屋  

  19. Plant Receptor Kinases Involved in Perception of Peptide Hormones Invited International conference

    松林嘉克

    International Conference of the Korean Society for Molecular and Cellular Biology 2022  2022.9.29  Korean Society for Molecular and Cellular Biology

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    Event date： 2022.9

    Language：English   Presentation type：Symposium, workshop panel (nominated)  

    Venue：済州島   Country：Korea, Republic of  

  20. 植物の窒素吸収を制御する長距離シグナル伝達 Invited

    大久保祐里，松林嘉克

    日本植物学会大86回大会シンポジウム  2022.9  日本植物学会

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    Event date： 2022.9

    Language：Japanese   Presentation type：Symposium, workshop panel (nominated)  

    Venue：京都  

  21. 植物の窒素吸収を制御する長距離移行ペプチド群 Invited

    大久保祐里，松林嘉克

    第44回日本分子生物学会年会ワークショップ 「不均一環境変動に対する植物の情報統御機構」  2021.12.3  日本分子生物学会

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    Event date： 2021.12

    Language：Japanese   Presentation type：Symposium, workshop panel (nominated)  

    Venue：横浜  

  22. 理研バイオリソースに支えられた植物ペプチドホルモン研究 Invited

    松林嘉克

    理研BRC設立20周年記念シンポジウム  2021.10.20  理化学研究所

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    Event date： 2021.10

    Language：Japanese   Presentation type：Symposium, workshop panel (nominated)  

  23. Roles of two structurally distinct sulfated peptides, PSK and PSY1, in Arabidopsis growth and development International conference

    19th International Conference on Plant Growth Substances 

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    Event date： 2007.7

    Language：English   Presentation type：Oral presentation (invited, special)  

  24. Physiological functions of two structurally distinct tyrosine-sulfated peptides in Arabidopsis growth and development International conference

    18th International Conference on Arabidopsis Research 

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    Event date： 2007.6

    Language：English   Presentation type：Oral presentation (invited, special)  

  25. An LRR receptor kinase involved in the perception of a peptide plant hormone, phytosulfokine

    Protein Phosphorylation in Plant Signaling 2005 

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    Event date： 2005.10

    Language：English   Presentation type：Oral presentation (invited, special)  

    Country：Japan  

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Research Project for Joint Research, Competitive Funding, etc. 1

  1. 新規ペプチドリガンド-受容体ペアの探索を基軸とした植物成長の分子機構解析

    2010.2 - 2013.5

    先端研究助成基金助成金（最先端・次世代研究開発支援プログラム） 

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    Grant type：Competitive

    本研究は，ホルモンなどの物質による植物成長の人為的制御を目指して，新しい植物ホルモンやその受容体を見つけ出すことを主たる目的とした．研究の結果，根端メリステムの細胞分裂活性の制御に関与する新しいペプチドホルモンの発見とその受容体の同定，側根形成への関与が示唆されるペプチドの受容体の同定，マメ科植物の根粒数の調節に関与する糖ペプチドの構造解明と受容体への結合の証明などの成果を得た．また，一部のペプチドやタンパク質の活性化に必要なヒドロキシプロリン残基のアラビノシル化修飾を担う糖転移酵素の精製・同定に成功した．いずれもこれまで全く知られていなかった新しい分子やしくみの発見であり，農作物の品種改良や生育調節に応用可能であると考えられる．

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KAKENHI (Grants-in-Aid for Scientific Research) 14

  1. Symplast; intercellular communication mechanism in plants under environmental changes

    Grant number：25H01340  2025.4 - 2030.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Transformative Research Areas (A)

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    Authorship：Coinvestigator(s)  Grant type：Competitive

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  2. 原形質連絡構成分子およびシンプラスト移行性分子の探索

    Grant number：25H01342  2025.4 - 2030.3

    科学研究費助成事業  学術変革領域研究(A)

    松林 嘉克

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    Authorship：Principal investigator 

    Grant amount：\69160000 （ Direct Cost: \53200000 、 Indirect Cost：\15960000 ）

    植物には、篩管内を長距離移行する非分泌型ペプチド群によって、葉や根などの間で器官間コミュニケーションが行なわれていることが明らかになりつつある。本研究では、独自のスクリーニングにより、植物の器官間コミュニケーションに関わる新しいペプチドシグナルの発見を目指す。また、非分泌型ペプチド群の器官間移行には、細胞同士をつなぐ原形質連絡が重要であることも知られている。原形質連絡の分子透過性が亢進した変異体や原形質連絡数が減少した変異体において存在量が変動するタンパク質を探索することで、原形質連絡の形成機構や制御機構を明らかにする。

  3. Dissection of plant growth and environmental response mechanisms through peptide signaling

    Grant number：23H05477  2023.4 - 2028.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (S)

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    Authorship：Principal investigator  Grant type：Competitive

    Grant amount：\202930000 （ Direct Cost: \156100000 、 Indirect Cost：\46830000 ）

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  4. Multi-layered regulatory system of plant resilience under fluctuating environment

    Grant number：20H05905  2020.11 - 2025.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Transformative Research Areas (A)

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    Authorship：Coinvestigator(s)  Grant type：Competitive

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  5. Adaptation Mechanism to Fluctuating Environment via Long-Range Signaling

    Grant number：20H05907  2020.11 - 2025.3

    Grants-in-Aid for Scientific Research  Grant-in-Aid for Transformative Research Areas (A)

    Matsubayashi Yoshikatsu

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    Authorship：Principal investigator  Grant type：Competitive

    Grant amount：\60060000 （ Direct Cost: \46200000 、 Indirect Cost：\13860000 ）

    We revealed that CEPH, a phosphatase induced downstream of the shoot-to-root mobile nitrogen-demand signal CEPD1/2/CEPDL2, dephosphorylates Ser501 of NRT2.1, thereby promoting nitrate ion uptake activity in roots. Additionally, we discovered GrxS1/2/3/4/5/6/7/8, which act as nitrogen-sufficient signals, induced when nitrogen is sufficient in the shoot, translocate to the roots, and inhibit nitrate uptake. CEPD1/2/CEPDL2 and GrxS1/2/3/4/5/6/7/8 signals competitively bind to the transcription factor TGA1/4, dynamically regulating the transcription of target genes in response to nitrogen demand of plants.

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  6. Integrative system of autonomous environmental signal recognition and memorization for plant plasticity

    Grant number：20H05603  2020.4 - 2021.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

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    Authorship：Coinvestigator(s) 

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  7. Molecular dissection of peptide signaling in plants

    Grant number：18H05274  2018.6 - 2023.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (S)

    MATSUBAYASHI Yoshikatsu

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    Authorship：Principal investigator 

    Grant amount：\192530000 （ Direct Cost: \148100000 、 Indirect Cost：\44430000 ）

    The following results were obtained by a new approach that focuses on molecules and traces them back to mechanisms. (i) the discovery of a novel peptide hormone PSY and its receptor PSYR that controls stress response and growth switching in plant cells; (ii) the discovery of the phloem-mobile peptide signal CEPDL2, which communicates leaf nitrogen demand to the roots; (iii) the discovery of the protein phosphatase CEPH, which acts downstream of CEPDL2 and activates the nitrate transporter NRT2.1; and (iv) discovery of proteases that cleave peptide ligands that induce immune response from flagellin, the major protein constituting the bacterial flagellum.

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  8. Integrative system of autonomous environmental signal recognition and memorization for plant plasticity

    Grant number：15K21750  2015.11 - 2020.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

    Kinoshita Toshinori

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    Authorship：Coinvestigator(s) 

    In contrast to mobile animals, which can seek environments suitable for survival, immobile plants must use their flexible and efficient environmental response systems in order to respond rapidly to a variety of environmental changes. However, it remains unclear how plants control, assess, remember, and transmit information from the environment without brains or nerves. This area of study seeks answers to profound and longstanding biological questions. Our objective is to understand the mechanisms of plastic growth in response to the environment that are unique to plants. To this end, we seek to elucidate the long-distance signal transduction system ? which is completely different from those of animals ? that constitutes the local and autonomous environmental response system. Additionally, elucidation of the environmental memory system, which involves spatiotemporal caching of information via chromatin modifications, will be important for understanding plastic growth.

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  9. Integrative system of autonomous environmental signal recognition and memorization for plant plasticity

    Grant number：15H05955  2015.6 - 2020.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

    Toshinori Kinoshita

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    Authorship：Coinvestigator(s) 

    This new area of scientific study began with the goal of performing an integrated analysis of the dynamic environmental response control system in plants. This analysis includes clarifying the flexible and ingenious local and autonomous environmental response system evolved by immobile plants, which is completely different from those of animals, as well as a new spatiotemporal perspective. Specifically, we seek to analyze the long-distance signal transduction system of plants, as well as the environmental memory system that caches information via chromatin modifications. Actually, we have obtained exciting results regarding the dynamic environmental response control system in plants.

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  10. Response mechanisms to a fluctuating environment through long-distance signaling

    Grant number：15H05957  2015.6 - 2020.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

    Matsubayashi Yoshikatsu

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    Authorship：Principal investigator 

    Grant amount：\95940000 （ Direct Cost: \73800000 、 Indirect Cost：\22140000 ）

    Nitrogen (N) is an essential macronutrient that plays a crucial role throughout plant development, but soil nitrate availability fluctuates temporally and spatially. We identified that N starvation-induced peptide CEP acts as a root-derived ascending N-demand signal. CEP is recognized in the shoot by CEP receptor, that leads to the production of non-secreted polypeptide, CEPD. CEPD acts as a descending signal to the root and ultimately upregulates nitrate transporter NRT2.1 gene in the roots. Thus, CEP induced on one side of the roots by local N starvation is able to mediate upregulation of NRT2.1 in the distant part of the roots. In addition to this, plants modulate the efficiency of root N acquisition in response to shoot N demand by CEPD-like 2, which is upregulated in the leaf vasculature in response to decreased shoot N status. Our findings provide new insights into the molecular basis of plant adaptation to a dynamically fluctuating N environment.

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  11. Molecular dissection of plant development and cell-to-cell signaling mediated by posttranslationally modified peptides

    Grant number：25221105  2013.5 - 2018.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (S)

    Matsubayashi Yoshikatsu

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    Authorship：Principal investigator 

    Grant amount：\209820000 （ Direct Cost: \161400000 、 Indirect Cost：\48420000 ）

    Our goal in this project is to uncover the mechanisms by which plant development is regulated through identification of novel peptide hormones and their specific receptors. In plants, small post-translationally modified peptides constitute the largest group of peptide hormones. We explored this type of peptide hormone by in silico gene screening coupled with biochemical and functional analysis, which led to the identification of two types of novel peptide hormones such as CEP family peptides regulating long-distance nitrogen demand signaling and CIF family peptides required for contiguous Casparian strip formation in roots. We also identified receptors for RGF family peptides involved in root meristem development.

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  12. 分泌因子および受容体キナーゼを介した情報伝達機構

    2007.9 - 2010.1

    科学研究費補助金  特定領域研究

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    Authorship：Principal investigator 

    ペプチドホルモンの翻訳後修飾のひとつであるチロシン硫酸化に関与する酵素（tyrosylprotein sulfotransferase: TPST）の同定に成功し，この酵素の欠損株では，根端の幹細胞が維持されず，根が極端に短くなることを見出した．さらに，この表現型の原因のひとつとなっていた新しいチロシン硫酸化ペプチドホルモン（root meristem growth factor: RGF）の同定にも成功した．RGFは，根端メリステム幹細胞ニッチの維持およびメリステム活性の制御に関与している．また，液体培地に分泌された微量の分泌型ペプチドの効率的な抽出・構造解析法を確立し，新規ペプチドホルモンの候補を複数見出した．この手法を応用したCLV3ペプチドの構造解析の過程で，Hyp側鎖への糖鎖付加（トリアラビノシル化）という翻訳後修飾を見出し，この修飾がCLV3の生物活性や受容体結合活性を高める重要な働きをしていることを突き止めた．

  13. 翻訳後修飾に着目した分泌型ペプチドミクス

    2006.4 - 2009.3

    科学研究費補助金  若手研究(A)

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    Authorship：Principal investigator 

    植物のかたちづくりには様々な内生のペプチドホルモンが関与している．本研究では，in silicoおよび生化学的解析を統合して，翻訳後修飾ペプチドホルモン候補を探索し，その生理機能解析を行なった．その結果，複数の新規ペプチドホルモン候補を見出すことに成功した．また，翻訳後修飾のひとつであるチロシン硫酸化酵素の精製とクローニングを行ない，その遺伝子破壊株が様々な成長異常を示すことを見出した．

  14. 高等植物におけるタンパク質チロシン硫酸化機構の解明

    2004.4 - 2006.3

    科学研究費補助金  若手研究(B)

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    Authorship：Principal investigator 

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Teaching Experience (On-campus) 3

  1. 生物学基礎Ⅱ

    2025

  2. 生物学基礎I

    2022

  3. 生理活性物質化学

    2009

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Teaching Experience (Off-campus) 6

  1. 大学院特別講義

    2025.11 （Tohoku University）

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    Level：Postgraduate 

  2. 大学院特別講義

    2023.12 （Kyushu University）

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    Level：Postgraduate 

  3. 大学院特別講義

    2023.9 （Okayama University）

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    Level：Postgraduate 

  4. 大学院特別講義

    2022.12 （Kyoto University）

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    Level：Postgraduate 

  5. 大学院特別講義

    2007.4 - 2008.3 （東京大学大学院農学生命科学研究科）

  6. 大学院特別講義

    2006.4 - 2007.3 （京都大学大学院理学研究科）

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Social Contribution 1

  1. 新規ペプチドホルモンの発見から明らかになる植物の巧みな成長・環境応答機構

    Role(s)：Appearance

    愛知県立岡崎高等学校・あいち科学技術教育推進協議会  科学三昧inあいち2022  2022.12

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Media Coverage 4

  1. 植物の成長とストレス応答を制御 Newspaper, magazine

    日経産業新聞  日経産業新聞  2022.10

  2. 名古屋大が新ホルモン発見 Newspaper, magazine

    科学新聞  科学新聞  2022.10

  3. ストレスに応じ植物の成長調整 Newspaper, magazine

    読売新聞  読売新聞  夕刊  2022.10

  4. 成長を続けるかストレス防御か　植物の切り替えホルモン発見 Newspaper, magazine

    中日新聞  中日新聞  朝刊  2022.10

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