Updated on 2024/04/02

写真a

 
YOSHIOKA, Hirofumi
 
Organization
Graduate School of Bioagricultural Sciences Department of Plant Production Sciences Associate professor
Graduate School
Graduate School of Bioagricultural Sciences
Undergraduate School
School of Agricultural Sciences
Title
Associate professor
Contact information
メールアドレス

Degree 1

  1. Ph.D. ( 1993.5   Nagoya University ) 

Research Areas 3

  1. Others / Others  / Plant Pathology

  2. Others / Others  / Plant immunology

  3. Environmental Science/Agriculture Science / Plant protection science

Current Research Project and SDGs 6

  1. Studies on plant immune system in plant-pathogen interactions.

  2. Regulatory mechanisms of NO and ROS generation and role in plant immunity

  3. Regulatory mechanisms of plant defense response by MAP kinase cascade.

  4. Production of transgenic plants showing resistance to potato late blight.

  5. Studies on the mode of action of host-selective toxin produced by plant pathtogen.

  6. イネ虫害抵抗性遺伝子の分子作用機構に関する研究

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Research History 13

  1. Nagoya University   Graduate School of Bioagricultural Sciences   Associate professor

    2018.4

  2. Nagoya University   Graduate School of Bioagricultural Sciences Department of Biological Mechanisms and Functions   Associate professor

    2007.4 - 2018.3

  3. 名古屋大学大学院生命農学研究科准教授

    2007.4

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    Country:Japan

  4. Associate Professor, Graduate School of Bioagricultural Sciences, Nagoya University

    2005.4 - 2007.3

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    Country:Japan

  5. Associate Professor, Graduate School of Bioagricultural Sciences, Nagoya University

    2005.4 - 2007.3

  6. The Sainsbury Laboratory, John Innes Centre

    2001.6 - 2001.11

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    Country:Japan

  7. The Sainsbury Laboratory, John Innes Centre

    2001.6 - 2001.11

  8. Assistant Professor, Graduate School of Bioagricultural Sciences, Nagoya University

    1999.4 - 2005.3

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    Country:Japan

  9. Assistant Professor, Graduate School of Bioagricultural Sciences, Nagoya University

    1999.4 - 2005.3

  10. Department of Agronomy and Plant Genetics, University of Minnesota

    1994.7 - 1996.6

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    Country:Japan

  11. Department of Agronomy and Plant Genetics, University of Minnesota

    1994.7 - 1996.6

  12. Assistant Professor, School of Agricultural Sciences, Nagoya University

    1991.7 - 1999.3

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    Country:Japan

  13. Assistant Professor, School of Agricultural Sciences, Nagoya University

    1991.7 - 1999.3

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Education 3

  1. Okayama University   Graduate School, Division of National Science and Technology

    - 1991.6

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    Country: Japan

  2. Mie University   Graduate School, Division of Agriculture

    - 1989.3

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    Country: Japan

  3. Mie University   Faculty of Agriculture

    - 1987

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    Country: Japan

Professional Memberships 14

  1. 日本植物病理学会

    2014.1 - 2018.12

  2. 日本植物生理学会

    2016.1 - 2019.12

  3. American Phytopathological Society

  4. International Society for Molecular Plant-Microbe Interactions

  5. American Society of Plant Biologists

  6. 関西病害虫研究会

  7. 植物微生物研究会

  8. 関西病害虫研究会

  9. 植物微生物研究会

  10. 日本植物病理学会

  11. 日本植物生理学会

  12. International Society for Molecular Plant-Microbe Interactions

  13. American Society of Plant Biologists

  14. American Phytopathological Society

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Awards 3

  1. 日本植物病理学会学術奨励賞

    1997.3   日本植物病理学会  

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    Country:Japan

  2. 日本農学進歩賞

    2003.11   財団法人農学会  

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    Country:Japan

  3. 日本植物病理学会論文賞

    2005.3   日本植物病理学  

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    Country:Japan

 

Papers 238

  1. NADPH oxidase-mediated sulfenylation of cysteine derivatives regulates plant immunity Invited Reviewed

    Hino, Y., Inada, T., Yoshioka, M. and Yoshioka, H.

    Journal of Experimental Botany     2024

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    Authorship:Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: https://doi.org/10.1093/jxb/erae111

  2. Dynamics of plant immune MAPK activity and ROS signaling in response to invaders. Invited Reviewed

    Yoshioka, H., Hino, Y., Iwata, K., Ogawa, T., Yoshioka, M., Ishihama, N. and Adachi, H.

    Physiological and Molecular Plant Pathology     2023.3

  3. Oral RNAi of diap1 results in rapid reduction of damage to potatoes in Henosepilachna vigintioctopunctata. Reviewed

    Chikami, Y., Kawaguchi, H., Suzuki, T., Yoshioka, H., Sato, Y., Yaginuma, T. and Niimi, T.

    Journal of Pest Science   Vol. 94   page: 505 - 515   2021.4

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: doi.org/10.1007/s10340-020-01276-w

  4. Role of salicylic acid glucosyltransferase in balancing growth and defence for optimum plant fitness. Reviewed

    Kobayashi, Y., Fukuzawa, N., Hyodo, A., Kim, H., Mashiyama, S., Ogihara, T., Yoshioka, H., Matsuura, H., Masuta, C., Matsumura, T. and Takeshita, M.

    Molecular Plant Pathology   Vol. 21   page: 429-442.   2020.3

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: doi: 10.1111/mpp.12906.

  5. RNAi of sesquiterpene cyclase gene for phytoalexin production impairs in pre- and postinvasive resistance to potato blight pathogens. Reviewed

    Yoshioka, M, Adachi, I, Sato, Y, Doke, N, Kondo, T, Yoshioka, H

    Molecular Plant Pathology   Vol. 20 ( 7 ) page: 907-922. - 922   2019.4

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1111/mpp.12802

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  6. An NADPH oxidase RBOH functions in rice roots during lysigenous aerenchyma formation under oxygen-deficient conditions. Reviewed

    Yamauchi, T., Yoshioka, M., Fukazawa, A., Mori, H., Nishizawa, N.K., Tsutsumi, N., Yoshioka, H. and Nakazono, M.

    The Plant Cell   Vol. 29   page: 775-790.   2017.4

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1105/tpc.16.00976

  7. Nicotiana benthamiana MAPK-WRKY pathway confers resistance to a necrotrophic pathogen Botrytis cinerea. Reviewed

    Adachi, H., Ishihama, N., Nakano, T., Yoshioka, M. and Yoshioka, H.

    Plant Signaling & Behavior   Vol. 6 ( 11 ) page: e1183085   2016.5

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1080/15592324.2016.1183085

  8. Hierarchical regulation of NADPH oxidase by protein kinases in plant immunity. Invited Reviewed

    Yoshioka, H., Adachi, H., Nakano, T., Miyagawa, N., Ishihama, N., Asai, S. and Yoshioka, M.

    Physiological and Molecular Plant Pathology   Vol. 95   page: 20-26.   2016.5

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    Authorship:Lead author   Language:English  

    DOI: 10.1016/j.pmpp.2016.03.004

  9. SGT1 contributes to maintaining protein levels of MEK2DD to facilitate hypersensitive response-like cell death in Nicotiana benthamiana. Reviewed

    Ichimura, K., Shinzato, T., Edaki, M., Yoshioka, H. and Shirasu, K.

    Physiological and Molecular Plant Patholology   Vol. 94   page: 47-52.   2016.4

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.pmpp.2016.04.001

  10. *WRKY transcription factors phosphorylated by MAPK regulate a plant immune NADPH oxidase in Nicotiana benthamiana. Reviewed

    Adachi, H., Nakano, T., Miyagawa, N., Ishihama, N., Yoshioka, M., Katou, Y., Yaeno, T., Shirasu, K. and Yoshioka, H.

    The Plant Cell   Vol. 27 ( 9 ) page: 2645-2663.   2015.10

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1105/tpc.15.00213

  11. Molecular Characterization of LjABCG1, an ATP-Binding Cassette Protein in Lotus japonicus Reviewed

    Akifumi Sugiyama, Shoju Fukuda, Kojiro Takanashi, Miki Yoshioka, Hirofumi Yoshioka, Yoshihiro Narusaka, Mari Narusaka, Mikiko Kojima, Hitoshi Sakakibara, Nobukazu Shitan, Shusei Sato, Satoshi Tabata, Masayoshi Kawaguchi, Kazufumi Yazaki

    PLOS ONE   Vol. 10 ( 9 ) page: e0139127.   2015.9

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:PUBLIC LIBRARY SCIENCE  

    LjABCG1, a full-size ABCG subfamily of ATP-binding cassette proteins of a model legume, Lotus japonicus, was reported as a gene highly expressed during the early stages of nodulation, but have not been characterized in detail. In this study we showed that the induction of LjABCG1 expression was remarkable by methyl jasmonate treatment, and reporter gene experiments indicated that LjABCG1 was strongly expressed in the nodule parenchyma and cell layers adjacent to the root vascular tissue toward the nodule. LjABCG1 was suggested to be localized at the plasma membrane based on the fractionation of microsomal membranes as well as separation via aqueous two-phase partitioning. The physiological functions of LjABCG1 in symbiosis and pathogenesis were analyzed in homologous and heterologous systems. LjABCG1 knock-down L. japonicus plants did not show clear phenotypic differences in nodule formation, and not in defense against Pseudomonas syringae, either. In contrast, when LjABCG1 was expressed in the Arabidopsis pdr8-1 mutant, the penetration frequency of Phytophthora infestans, a potato late blight pathogen, was significantly reduced in LjABCG1/pdr8-1 than in pdr8-1 plants. This finding indicated that LjABCG1, at least partially, complemented the phenotype of pdr8 in Arabidopsis, suggesting the multiple roles of this protein in plant-microbe interactions.

    DOI: 10.1371/journal.pone.0139127

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  12. Cell death-inducing stresses are required for defense activation in DS1-phosphatidic acid phosphatase-silenced Nicotiana benthamiana. Reviewed

    Nakano, M., Yoshioka, H., Ohnishi, K., Hikichi, Y. and Kiba, A.

    Journal of Plant Physiology   Vol. 184   page: 15-19.   2015.7

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    DOI: 10.1016/j.jplph.2015.06.007

  13. *A receptor pair with an integrated decoy converts pathogen disabling of transcription factors to immunity. Reviewed

    Le Roux, C., Huet, G., Jauneau, A., Camborde, L., Trémousaygue, D., Kraut, A., Zhou, B., Levaillant, M., Adachi, H., Yoshioka, H., Raffaele, S., Berthomé, R., Couté, Y., Parker, J.E. and Deslandes, L.

    Cell   Vol. 161   page: 1074-1088.   2015.5

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    DOI: doi.org/10.1016/j.cell.2015.04.025

  14. Kinase-mediated orchestration of NADPH oxidase in plant immunity. Invited Reviewed

    Adachi, H. and Yoshioka, H.

    Briefings in Functional Genomics   Vol. 14 ( 4 ) page: 253-259.   2015.3

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    Language:English  

    DOI: 10.1093/bfgp/elv004

  15. リン酸化反応が制御するROSバーストの分子機構 Invited Reviewed

    吉岡博文・安達広明・石濱伸明・中野孝明・白石佑太郎・宮川典子・野村裕也・吉岡美樹・浅井秀太

    日本植物病理学会報   Vol. 81   page: 1-8.   2015.2

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    Authorship:Lead author   Language:Japanese  

  16. Map-based Cloning and Characterization of a Brown Planthopper Resistance Gene BPH26 from Oryza sativa L. ssp indica Cultivar ADR52 Reviewed

    Yasumori Tamura, Makoto Hattori, Hirofumi Yoshioka, Miki Yoshioka, Akira Takahashi, Jianzhong Wu, Naoki Sentoku, Hideshi Yasui

    SCIENTIFIC REPORTS   Vol. 4   page: 5872   2014.7

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:NATURE PUBLISHING GROUP  

    The brown planthopper (BPH) is the most serious insect pest of rice in Asia. The indica rice cultivar ADR52 carries two BPH resistance genes, BPH26 (BROWN PLANTHOPPER RESISTANCE 26) and BPH25. Map-based cloning of BPH26 revealed that BPH26 encodes a coiled-coil-nucleotide-binding-site-leucine-rich repeat (CC-NBS-LRR) protein. BPH26 mediated sucking inhibition in the phloem sieve element. BPH26 was identical to BPH2 on the basis of DNA sequence analysis and feeding ability of the BPH2-virulent biotype of BPH. BPH2 was widely incorporated in elite rice cultivars and was well-cultivated in many Asian countries as a favorable gene resource in rice breeding against BPH. However, BPH2 was rendered ineffective by a virulent biotype of BPH in rice fields in Asia. In this study, we suggest that BPH2 can be reused by combining with other BPH resistance genes, such as BPH25, to ensure durable resistance to BPH.

    DOI: 10.1038/srep05872

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  17. SEC14 phospholipid transfer protein is involved in lipid signaling-mediated plant immune responses in Nicotiana benthamiana. Reviewed

    Kiba, A., Galis, I., Hojo, Y., Ohnishi, K., Yoshioka, H. and Hikichi, Y.

    PLoS ONE   Vol. 9 ( 5 ) page: e98150   2014.5

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    DOI: 10.1371/journal.pone

  18. Silencing of DS2 aminoacylase-like genes confirms basal resistance to Phytophthora infestans in Nicotiana benthamiana. Reviewed

    Nakano, M., Nishihara, M., Yoshioka, H., Ohnishi, K., Hikichi, Y. and Kiba, A.

    Plant Signal. Behav.   Vol. 9 ( 2 ) page: e28004   2014.2

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    DOI: doi:10.4161/psb.28004

  19. Suppression of DS1 phosphatidic acid phosphatase confirms resistance to Ralstonia solanacearum in Nicotiana benthamiana. Reviewed

    Nakano, M., Nishihara, M., Yoshioka, H., Takahashi, H., Sawasaki, T., Ohnishi, K., Hikichi, Y. and Kiba, A.

    PLoS ONE   Vol. 8 ( 9 ) page: e75124.   2013.9

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  20. A translationally controlled tumor protein negatively regulates the hypersensitive response in Nicotiana benthamiana. Reviewed

    Gupta, M., Yoshioka, H., Ohnishi, K., Mizumoto, H., Hikichi, Y. and Kiba, A.

    Plant and Cell Physiology   Vol. 54 ( 8 ) page: 1403-1414.   2013.8

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  21. タンパク質のリン酸化によるROSバーストの分子機構 Invited

    吉岡博文・安達広明・石濱伸明・中野孝明・白石佑太郎・宮川典子・野村裕也・吉岡美樹・浅井秀太

    植物−微生物相互作用とビオトロフ感染   Vol. 48   page: 105-114.   2013.8

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    Authorship:Lead author   Language:Japanese  

  22. Ethylene responsive AP2/ERF transcription factor MACD1 participates in phytotoxin-triggered programmed cell death. Reviewed

    Mase, K., Ishihama, N., Mori, H., Takahashi, H., Kaminaka, H., Kodama, M. and Yoshioka, H.

    Molecular Plant-Microbe Interactions   Vol. 26   page: 868-879.   2013.7

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  23. *The variable domain of a plant calcium-dependent protein kinase (CDPK) confers subcellular localization and substrate recognition for NADPH oxidase. Reviewed

    Asai, S., Ichikawa, T., Nomura, H., Kobayashi, M., Kamiyoshihara, Y., Mori, H., Kadota, Y., Zipfel, C., Jones, J.D.G. and Yoshioka, H.

    The Journal of Biological Chemistry   Vol. 288   page: 14332-14340.   2013.5

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  24. *A receptor-like cytoplasmic kinase targeted by a plant pathogen effector is directly phosphorylated by the chitin receptor and mediates rice immunity. Reviewed

    Yamaguchi, K., Yamada, K., Ishikawa, K., Yoshimura, S., Hayashi, N., Uchihashi, K., Ishihama, N., Kishi-Kaboshi, M., Takahashi, A., Tsuge, S., Ochiai, H., Tada, Y., Shimamoto, K., Yoshioka, H. and Kawasaki, T.

    Cell Host & Microbe   Vol. 13   page: 347-357.   2013.3

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  25. Nitric oxide as a mediator for defense responses. Invited Reviewed

    Bellin, D., Asai, S., Delledonne, M. and Yoshioka, H.

    Molecular Plant-Microbe Interactions   Vol. 26 ( 3 ) page: 271-277.   2013.2

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  26. *StCDPK5 confers resistance to late blight pathogen but increases susceptibility to early blight pathogen in potato via reactive oxygen species burst. Reviewed

    Kobayashi, M., Yoshioka, M., Asai, S., Nomura, H., Kuchimura, K., Mori, H., Doke, N. and Yoshioka, H.

    New Phytologist   Vol. 196   page: 223–237.   2012.10

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  27. *Post-translational regulation of WRKY transcription factors in plant immunity. Invited Reviewed

    Ishihama, N. and Yoshioka, H.

    Current Opinion in Plant Biology   Vol. 15   page: 431-437.   2012.8

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  28. Isolation and characterization of an asparagine-rich protein that regulates hypersensitive cell death-mediated resistance in Nicotiana plants. Reviewed

    Komori, D., Nishihara, M., Takahashi, A., Gupta, M., Yoshioka, H., Mizumoto, H., Ohnishi, K., Hikichi, Y. and Kiba, A.

    Plant Biotechnology   Vol. 29   page: 293-300.   2012.7

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  29. Ethylene signaling pathway and MAPK cascades are required for AAL toxin-induced programmed cell death. Reviewed

    Mase, K., Mizuno, T., Ishihama, N., Fujii, T., Mori, H., Kodama, M. and Yoshioka, H.

    Molecular Plant-Microbe Interactions   Vol. 25   page: 1015-1025.   2012.7

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  30. *Chloroplast-mediated activation of plant immune signalling in Arabidopsis. Reviewed

    Nomura, H., Komori, T., Uemura, S., Kanda, Y., Shimotani, K., Nakai, K., Furuichi, T., Takebayashi, K., Sugimoto, T., Sano, S., Suwastika, I.N., Fukusaki, E., Yoshioka, H., Nakahira, Y. and Shiina, T.

    Nature Communications   Vol. 3   page: 926.   2012.6

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  31. A novel Sec14 phospholipid transfer protein from Nicotiana benthamiana is up-regulated in response to Ralstonia solanacearum infection, pathogen associated molecular patterns and effector molecules and involved in plant immunity. Reviewed

    Kiba, A., Nakano, M., Vincent-Pope, P., Takahashi, H., Sawasaki, T., Endo, Y., Ohnishi, K., Yoshioka, H. and Hikichi, Y.

    Journal of Plant Physiology   Vol. 169 ( 10 ) page: 1017-1022.   2012.6

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    DOI: org/10.1016/j.jplph.2012.04.002

  32. Cell death of Nicotiana benthamiana is induced by secreted protein NIS1 of Colletotrichum orbiculare and is suppressed by a homologue of CgDN3. Reviewed

    Yoshino, K., Irieda, H., Sugimoto, F., Yoshioka, H., Okuno, T. and Takano, Y.

    Molecular Plant-Microbe Interactions   Vol. 25   page: 625-636.   2012.4

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  33. Regulatory mechanisms of nitric oxide and reactive oxygen species generation and their role in plant immunity. Invited Reviewed

    Yoshioka, H., Mase, K., Yoshioka, M., Kobayashi, M. and Asai, S.

    Nitric Oxide   Vol. 25   page: 216-221.   2011.8

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    Authorship:Lead author   Language:English  

    DOI: 10.1016/j.niox.2010.12.008

  34. A plastidic glucose-6-phosphate dehydrogenase is responsible for hypersensitive response cell death and reactive oxygen species production Reviewed

    Shuta Asai, Miki Yoshioka, Hironari Nomura, Chiyori Tone, Kazumi Nakajima, Eiichi Nakane, Noriyuki Doke, Hirofumi Yoshioka

    JOURNAL OF GENERAL PLANT PATHOLOGY   Vol. 77 ( 3 ) page: 152 - 162   2011.5

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    Glucose-6-phosphate dehydrogenase (G6PDH) has been implicated in the supply of reduced nicotine amide cofactors for resistance to biotic and abiotic stresses. Here, we show participation of the plastidic P2 isoform of G6PDH in plant immunity. A cytosolic isoform (NbG6PDH-Cyto) and two plastidic isoforms (NbG6PDH-P1 and NbG6PDH-P2) cloned from Nicotiana benthamiana were localized in cytosol and chloroplasts, respectively. Hypersensitive response (HR) cell death and NADPH oxidase (RBOH; respiratory burst oxidase homolog)-dependent reactive oxygen species (ROS) production after recognition of INF1 elicitin, secreted by oomycete Phytophthora infestans, decreased in NbG6PDH-P2-silenced plants, but not in NbG6PDH-Cyto- and NbG6PDH-P1-silenced plants. Silencing of the cytosolic NAD kinase NbNADK1, which phosphorylates NADH to form NADPH, compromised HR cell death and ROS production, and concomitant silencing with NbG6PDH-P2 reduced HR cell death and ROS to levels near those in NbG6PDH-P2-silenced plants. Similarly, silencing NbG6PDH-P2 and NbNADK1 resulted in high susceptibility to P. infestans. These results suggest that NADPH produced by the P2 isoform of G6PDH in chloroplasts is responsible for HR cell death and ROS production mediated by RBOH and that NbNADK1 is involved in this pathway.

    DOI: 10.1007/s10327-011-0304-3

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  35. *Phosphorylation of the Nicotiana benthamiana WRKY8 transcription factor by MAPK functions in the defense response. Reviewed

    Ishihama, N., Yamada, R., Yoshioka, M., Katou, S. and Yoshioka, H.

    The Plant Cell   Vol. 23   page: 1153-1170.   2011.3

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    DOI: 10.1105/tpc.110.081794

  36. NINJAはコリプレッサーTOPLESSとジャスモン酸シグナルをつなぐ―植物ホルモンの受容による新しい転写制御機構の役割は? Invited

    野村裕也・吉岡博文

    化学と生物   Vol. 49   page: 85-87   2011.2

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    Language:Japanese  

  37. S-glycoprotein-like protein regulates defense responses in Nicotiana plants against Ralstonia solanacearum. Reviewed

    Maimbo, M., Ohnishi, K., Hikichi, Y., Yoshioka, H. and Kiba, A.

    Plant Physiology   Vol. 152   page: 2023-2035   2010

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  38. Ca2+-dependent protein kinases and their substrate HsfB2a are differently involved in the heat response signaling pathway in Arabidopsis. Reviewed

    Nagamangala Kanchiswamy, C., Maffei, M.E., Yoshioka, H., Sawasaki, T. and Arimura, G.

    Plant Biotechnology   Vol. 27   page: 469-473   2010

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  39. Regulation of Arabidopsis defense responses against Spodoptera littoralis by CPK-mediated calcium signaling. Reviewed

    Nagamangala Kanchiswamy, C., Takahashi, H., Quadro, S., Maffei, M.E., Bossi, S., Bertea, C., Zebelo, S.A., Muroi, A., Ishihama, N., Yoshioka, H., Boland, W., Takabayashi, J., Endo, Y., Sawasaki, T. and Arimura, G.

    BMC Plant Biology   Vol. 10   page: 97   2010

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  40. Role of nitric oxide and reactive oxygen species in disease resistance to necrotrophic pathogens. Invited Reviewed

    Asai, S., Mase, K. and Yoshioka, H.

    Plant Signaling and Behavior   Vol. 5   page: 872-874   2010

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  41. *A key enzyme for flavin synthesis is required for nitric oxide and reactive oxygen species production in disease resistance. Reviewed

    Asai, S., Mase, K. and Yoshioka, H.

    The Plant Journal   Vol. 62   page: 911-924   2010

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  42. Analyses of the cis-regulatory regions responsible for the transcriptional activation of the N resistance gene by Tobacco mosaic virus. Reviewed

    Kobayashi, M., Ishihama, N., Yoshioka, H., Takabatake, R., Tsuda, S., Seo, S., Ohashi, Y. and Mitsuhara, I.

    Journal of Phytopathology   Vol. 158   page: 826-828.   2010

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  43. 病害抵抗性・感受性決定に関わる植物の感染応答機構 Invited

    木場章範・中野真人・伊藤 慎・Maimbo, M.・大西浩平・吉岡博文・水本祐之・曵地康史

    農業現場の問題解決に向けた感染生理学   Vol. 46   page: 1-8   2010

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  44. Nitric oxide as a partner of reactive oxygen species participates in disease resistance to necrotrophic pathogen Botrytis cinerea in Nicotiana benthamiana. Reviewed

    Asai, S. and Yoshioka, H.

    Molecular Plant-Microbe Interactions   Vol. 22   page: 619-629   2009

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  45. Molecular mechanisms of generation for nitric oxide and reactive oxygen species, and role of the radical burst in plant immunity. Invited Reviewed

    Yoshioka, H., Asai, S., Yoshioka, M. and Kobayashi, M.

    Molecules and Cells   Vol. 28   page: 321-329   2009

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  46. *The Colletotrichum orbiculare ssd1 mutant enhances Nicotiana benthamiana basal resistance by activating a mitogen-activated protein kinase pathway. Reviewed

    Tanaka, S., Ishihama, N., Yoshioka, H., Huser, A., O'Connell, R., Tsuji, G., Tsuge, S. and Kubo, Y.

    The Plant Cell   Vol. 21   page: 2517-2526   2009

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  47. INF1 elicitin activates jasmonic acid- and ethylene-mediated signalling pathways and induces resistance to bacterial wilt disease in tomato. Reviewed

    Kawamura, Y., Hase, S., Takenaka, S., Kanayama, Y., Yoshioka, H., Kamoun, S. and Takahashi, H.

    Journal of Phytopathology   Vol. 157   page: 287-297   2009

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  48. Vestigial and scalloped in the ladybird beetle: a conserved function in wing development and a novel function in pupal ecdysis. Reviewed

    Ohde, T., Matsumoto, M., Morita-Miwa, M., Matsuura, H., Yoshioka, H., Yaginuma, T. and Niimi, T.

    Insect Molecular Biology   Vol. 18   page: 571-581   2009

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  49. Involvement of NbNOA1 in NO production and defense responses in INF1-treated Nicotiana benthamiana. Reviewed

    Kato, H., Asai, S., Yamamoto-Katou, A., Yoshioka, H., Doke, N. and Kawakita, K.

    Journal of Genaral Plant Pathology   Vol. 74   page: 15-23   2008

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  50. Discovery of oxidative burst in the field of plant immunity: Looking back at the early pioneering works and towards the future development. Reviewed

    Yoshioka, H., Bouteau, F. and Kawano, T.

    Plant Signaling and Behavior   Vol. 3   page: 153-155   2008

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  51. The role of radical burst via MAPK signaling in plant immunity. Invited Reviewed

    Asai, S. and Yoshioka, H.

    Plant Signaling and Behavior   Vol. 3   page: 920-922   2008

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  52. *MAPK signaling regulates nitric oxide and NADPH oxidase-dependent oxidative bursts in Nicotiana benthamiana. Reviewed

    Asai, S., Ohta, K. and Yoshioka, H.

    The Plant Cell   Vol. 20   page: 1390-1406   2008

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  53. *Calcium-dependent protein kinases regulate the production of reactive oxygen species by potato NADPH oxidase. Reviewed

    Kobayashi, M., Ohura, I., Kawakita, K., Yokota, N., Fujiwara, M., Shimamoto, K., Doke, N. and Yoshioka, H.

    The Plant Cell   Vol. 19   page: 1065-1080   2007

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  54. Induction of a small heat shock protein and its functional roles in Nicotiana plants in the defense response against Ralstonia solanacearum. Reviewed

    Maimbo, M., Ohnishi, K., Hikichi, Y., Yoshioka, H. and Kiba, A.

    Plant Physiology   Vol. 145   page: 1588-1599   2007

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  55. *Regulation of rice NADPH oxidase by binding of Rac GTPase to its N-terminal extension. Reviewed

    Wong, H.L., Pinontoan, R., Hayashi, K., Tabata, R., Yaeno, T., Hasegawa, K., Kojima, C., Yoshioka, H., Iba, K., Kawasaki, T. and Shimamoto, K.

    The Plant Cell   Vol. 19   page: 4022-4034   2007

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  56. 植物免疫におけるMAPキナーゼとラジカルバースト.植物における環境と生物ストレスに対する応答 Invited

    吉岡博文・浅井秀太

    蛋白質核酸酵素 増刊号   Vol. 52   page: 667-672   2007

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  57. 植物免疫におけるラジカルバーストの分子機構 Invited

    吉岡博文・浅井秀太・間瀬圭介・市川達士・小林光智衣・小八重善裕

    植物-病原微生物の相互作用のダイナミズム   Vol. 43   page: 37-47   2007

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  58. 植物病原細菌の感染を制御する宿主応答機構~ Ralstonia solanacearum- Nicotiana属植物相互作用の解析~ Invited

    木場章範・Maimbo, M.・小森大輔・張 宛果・富山裕美・高橋彰子・大西浩平・吉岡博文・曳地康史

    植物-病原微生物の相互作用のダイナミズム   Vol. 43   page: 55-65   2007

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  59. Nitrate reductase is responsible for elicitin-induced nitric oxide production in Nicotiana benthamiana. Reviewed

    Yamamoto-Katou, A., Katou, S., Yoshioka, H., Doke, N. and Kawakita, K.

    Plant and Cell Physiology   Vol. 47   page: 726-735   2006

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  60. Peroxynitrite generation and tyrosine nitration in defense responses in tobacco BY-2 cells.

    Saito, S., Yamamoto-Katou, A., Yoshioka, H., Doke, N. and Kawakita, K.

    Plant and Cell Physiology   Vol. 47   page: 689-697   2006

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  61. The E3 ubiquitin ligase activity of Arabidopsis PLANT U-BOX17 and its functional tobacco homolog ACRE276 are required for cell death and defense. Reviewed

    Yang, C.-W., Gonzalez-Lamothe, R., Ewan, R.A., Rowland, O., Yoshioka, H., Shenton, M., Ye, H., O'Donnell, E., Jones, J.D.G. and Sadanandom, A.

    The Plant Cell   Vol. 18   page: 1084-1098   2006

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  62. Subcellular localization of Strboh proteins and NADPH-dependent O2--generating activity in potato tuber tissues. Reviewed

    Kobayashi, M., Kawakita, K., Maeshima, M., Doke, N. and Yoshioka, H.

    Journal of Experimental Botany   Vol. 57   page: 1373-1379   2006

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  63. Loss of AtPDR8, a plasma membrane ABC transporter of Arabidopsis thaliana, causes hypersensitive cell death upon pathogen infection. Reviewed

    Kobae, Y., Sekino, T., Yoshioka, H., Nakagawa, T., Martinoia, E. and Maeshima, M.

    Plant Cell Physiology   Vol. 47   page: 309-318   2006

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  64. Rewiring mitogen-activated protein kinase cascade by positive feedback confers potato blight resistance. Reviewed

    Yamamizo, C., Kuchimura, K., Kobayashi, A., Katou, S., Kawakita, K., Jones, J.D.G., Doke, N. and Yoshioka, H.

    Plant Physiology   Vol. 140   page: 681-692   2006

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  65. 植物の感染応答因子としてのNO. Invited

    川北一人・山本(加藤)文子・齋藤修平・加藤新平・加藤大明・浅井秀太・樹神博士・吉岡博文・道家紀志

    病原体の寄生戦略と植物の応答   Vol. 42   page: 11-22   2006

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  66. Involvement of PPS3 phosphorylated by elicitor-responsive mitogen-activated protein kinases in the regulation of plant cell death. Reviewed

    Katou, S., Yoshioka, H., Kawakita, K., Rowland, O., Jones, J.D.G., Mori, H. and Doke, N.

    Plant Physiology   Vol. 139   page: 1914-1926   2005

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  67. The Arabidopsis gene CAD1 controls programmed cell death in the plant immune system and encodes a protein containing a MACPF domain. Reviewed

    Morita-Yamamuro, C., Tsutsui, T., Sato, M., Yoshioka, H., Tamaoki, M., Ogawa, D. Matsuura, H., Yoshihara, T., Ikeda, A., Uyeda, I. and Yamaguchi, J.

    Plant and Cell Physiology   Vol. 46   page: 902-912   2005.1

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  68. Functional analysis of Avr9/Cf-9 rapidly elicited genes identifies a protein kinase, ACIK1, that is essential for full Cf-9-dependent disease resistance in tomato. Reviewed

    Rowland, O., Ludwig, A.A., Merrick, C.J., Baillieul, F., Tracy, F.E., Durrant, W.E., Fritz-Laylin, L., Nekrasov, V., Sjolander, K., Yoshioka, H. and Jones, J.D.G.

    The Plant Cell   Vol. 17   page: 295-310   2005.1

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  69. 植物における活性酸素の役割とその誘導.活性酸素,-生産と防御のメカニズム-. Invited

    吉岡博文・山溝千尋

    蛋白質 核酸 酵素   Vol. 50   page: 310-315   2005

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  70. 抵抗性遺伝子を介した防御応答におけるシグナル伝達機構. Invited

    吉岡博文・山溝千尋・小林光智衣・水野貴仁・川北一人・道家紀志

    植物・病原体相互反応における特異性決定機構   Vol. 41   page: 13-22   2005.8

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  71. Involvement of nitric oxide generation in hypersensitive cell death induced by elicitin in tobacco cell suspension culture.

    Yamamoto, A., Katou, S., Yoshioka, H., Doke, N. and Kawakita, K.

    Journal of Genaral Plant Pathology   Vol. 70   page: 85-92   2004.1

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  72. Elicitation of primary and secondary metabolism during defense in the potato. Reviewed

    Nakane, E., Kawakita, K., Doke, N. and Yoshioka, H.

    Journal of Genaral Plant Pathology   Vol. 69   page: 378-384   2003.1

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  73. Cytosolic HSP90 and HSP70 are essential components of INF1-mediated hypersensitive response and non-host resistance to Pseudomonas cichorii in Nicotiana benthamiana.

    Kanzaki, H., Saitoh, H., Ito, A., Fujisawa, S., Kamoun, S., Katou, S., Yoshioka, H. and Terauchi, R.

    Molecular Plant Pathology   Vol. 4   page: 383-391   2003.1

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  74. Disease stress-inducible genes of tobacco: expression profile of elicitor-responsive genes isolated by substractive hybridization. Reviewed

    Takemoto, D., Yoshioka, H., Doke, N. and Kawakita, K.

    Physiologia Plantarum   Vol. 118   page: 545-553   2003.1

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  75. Nitrate reductase, a nitric oxide-producing enzyme: induction by pathogen signals. Reviewed

    Yamamoto, A., Katou, S., Yoshioka, H., Doke, N. and Kawakita, K.

    Journal of Genaral Plant Pathology   Vol. 69   page: 218-229   2003.1

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  76. Functional analysis of potato mitogen-activated protein kinase kinase, StMEK1. Reviewed

    Katou, S., Yamamoto, A., Yoshioka. H., Kawakita, K. and Doke, N.

    Journal of General Plant Pathology   Vol. 69   page: 161-168   2003

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  77. *Nicotiana benthamiana gp91phox homologs NbrbohA and NbrbohB participate in H2O2 accumulation and resistance to Phytophthora infestans. Reviewed

    Yoshioka, H., Numata, N., Nakajima, K., Katou, S., Kawakita, K., Rowland, O., Jones, J.D.G. and Doke, N.

    The Plant Cell   Vol. 15   page: 706-718   2003.3

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  78. 植物免疫としてのオキシダティブバーストを探る,-MAPKカスケードが活性酸素の生成と細胞死を制御する-. Invited

    吉岡博文

    化学と生物   Vol. 41   page: 639-641   2003

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  79. 宿主植物の抵抗性制御と糸状菌病に対する耐病性強化の展望. Invited

    吉岡博文・口村和男・山溝千尋・池田直希・加藤新平・川北一人・小林 晃・道家紀志

    作物の耐病性強化戦略と植物-病原体相互作用の分子機構研究   Vol. 39   page: 101-110   2003

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  80. Induction of plant gp91 phox homolog by fungal cell wall, arachidonic acid, and salicylic acid in potato. Reviewed

    Yoshioka, H., Sugie, K., Park, H.-J., Maeda, H., Tsuda, N., Kawakita, K. and Doke, N.

    Molecular Plant-Microbe Interactions   Vol. 14   page: 725-736   2001.1

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  81. cDNA cloning of sesquiterpene cyclase and squalene synthase, and expression of the genes in potato tuber infected with Phytophthora infestans. Reviewed

    Yoshioka, H., Yamada, N. and Doke, N.

    Plant and Cell Physiology   Vol. 40 ( 9 ) page: 993-998   1999

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  82. Plasma membrane perturbation in association with calcium ion movement followed by fungal elicitor-stimulated oxidative burst and defense gene activation in potato tuber. Reviewed

    Miura, Y., Yoshioka, H., Park, H.-J., Kawakita, K. and Doke, N.

    Annals of the Phytopathological Society of Japan   Vol. 65   page: 447-453   1999.1

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  83. A 51 kDa protein kinase of potato activated with hyphal wall components from Phytophthora infestans. Reviewed

    Katou, S., Senda, K., Yoshioka, H., Doke, N. and Kawakita, K.

    Plant and Cell Physiology   Vol. 40   page: 825-831   1999.1

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  84. Effect of cytochalasin D on defense responses of potato tuber discs treated with hyphal wall components of Phytophthora infestans. Reviewed

    Takemoto, D., Maeda, H., Yoshioka, H., Doke, N. and Kawakita, K.

    Plant Science   Vol. 141   page: 219-226   1999.1

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  85. Aspartate aminotransferase in alfalfa nodules: Localization of mRNA during effective and ineffective nodules development and promoter analysis. Reviewed

    Yoshioka, H., Gregerson, R.G., Samac, D.A., Hoevens, K.C.M., Trepp, G., Gantt, J.S. and Vance, C.P.

    Molecular Plant-Microbe Interactions   Vol. 12   page: 263-274   1999.1

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  86. NADH-glutamate synthase in alfalfa root nodules: genetic regulation and cellular expression. Reviewed

    Trepp, G., van de Mortel, M., Yoshioka, H., Miller, S.S., Samac, D.A., Gantt, J.S. and Vance, C.P.

    Plant Physiology   Vol. 119   page: 817-828   1999.1

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  87. 誘導抵抗性関連研究の最前線. Invited

    吉岡博文

    日植病報バイオコントロール研究会レポート   Vol. 6   page: 19-28   1999

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  88. Physiological mechanisms of a sub-systemic oxidative burst triggered by elicitor-induced local oxidative burst in potato tuber slices. Reviewed

    Park, H.-J., Miura, Y., Kawakita, K., Yoshioka, H. and Doke, N.

    Plant and Cell Physiology   Vol. 39 ( 11 ) page: 1218-1225   1998

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  89. ジャガイモ塊茎における防御遺伝子発現とそのシグナル伝達. Invited

    吉岡博文・伊藤夢子・杉江顕一・三浦由雄・川北一人・道家紀志

    植物と病原体の情報応答機構     page: 49-108   1998

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  90. Nitrogen assimilation in alfalfa: Isolation and characterization of an asparagine synthetase gene showing enhanced expression in root nodules and dark-adapted leaves. Reviewed

    Shi, L., Twary, S.N., Yoshioka, H., Gregerson, R.G., Miller, S.S., Samac, D.A., Gantt, J.S., Unkefer, P.J. and Vance, C.P.

    The Plant Cell   Vol. 9   page: 1339-1356   1997.1

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  91. Analyses of phosphoenolpyruvate carboxylase gene structure and expression in alfalfa nodules. Reviewed

    Pathirana, S.M., Samac, D.A., Roeven, R., Yoshioka, H., Vance, C.P. and Gantt, J.S.

    The Plant Journal   Vol. 12   page: 293-304   1997.1

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  92. 植物病原糸状菌のサプレッサーによる宿主防御遺伝子の発現制御機構に関する研究. Invited

    吉岡博文

    日本植物病理学会報   Vol. 63   page: 141   1997.1

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  93. 植物の感染応答における情報伝達とホスホリパーゼ.植物感染生理学研究の現状と将来展望. Invited

    川北一人・仙田香織・鈴木真紀・吉岡博文・道家紀志

    植物感染生理学研究の現状と将来展望   Vol. 33   page: 134-142   1997

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  94. The oxidative burst protects plants against pathogen attack : mechanism and role as an emergency signal for plant bio-defence-a review. Invited Reviewed

    Doke, N., Miura, Y., Sanchez, L.M., Park, H.-J., Noritake, T., Yoshioka, H. and Kawakita, K.

    Gene   Vol. 179   page: 45-51   1996

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  95. A cytosolic phospholipase A2 from potato tissues appears to be patatin. Reviewed

    Senda, K., Yoshioka, H., Doke, N. and Kawakita, K.

    Plant and Cell Physiology   Vol. 37   page: 347-353   1996.1

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  96. Expression of genes for phenylalanine ammonia-lyase and 3-hydroxy-3-methylglutaryl CoA reductase in aged potato tubers infected with Phytophthora infestans. Reviewed

    Yoshioka, H., Miyabe, M., Hayakawa, Y. and Doke, N.

    Plant and Cell Physiology   Vol. 37   page: 81-90   1996.1

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  97. An autophotographic determination of the active oxygen generation in potato tuber discs during hypersensitive response to fungal infection or elicitor. Reviewed

    Miura, Y., Yoshioka, H. and Doke, N.

    Plant Science   Vol. 105   page: 45-52   1995.1

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  98. Suppression of the activation of chitinase and β-1,3-glucanase in pea epicotyls by eogenous suppressor from Pisam sativum. Reviewed

    Nasu, K., Yoshioka, H., Ichinose, Y., Yamada, T., Oku, H. and Shiraishi, T.

    Annals of the Phytopathological Society of Japan   Vol. 61   page: 13-17   1995.1

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  99. Suppression of phenylalanine ammonia-lyase mRNA accumulation by suppressors from Phytophthora infestans. Reviewed

    Yoshioka, H., Hayakawa, Y. and Doke, N.

    Annals of the Phytopathological Society of Japan   Vol. 61 ( 1 ) page: 7-12   1995

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  100. Suppressors : Determinants of specificity produced by plant pathogens. Invited Reviewed

    Shiraishi, T., Yamada, T., Saitoh, K., Kato, T., Toyoda, K., Yoshioka, H., Kim, H.-M., Ichinose, Y., Tahara, M. and Oku, H.

    Plant and Cell Physiology   Vol. 35 ( 8 ) page: 1107-1119   1994

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  101. Rapid accumulation of phenylalanine ammonia-lyase mRNA and 3-hydroxy-3-methylglutaryl CoA reductase mRNA by protein synthesis inhibitors. Reviewed

    Yoshioka, H. and Doke, N.

    Plant and Cell Physiology   Vol. 35 ( 8 ) page: 1257-1260   1994

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  102. Race-cultivar specificity in plant fungal disease and its molecular mechanisms. Invited

    Plant Cell Technology   Vol. 5 ( 1 ) page: 19-27   1993

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  103. An endogenous suppressor of the defense response in Pisum sativum. Reviewed

    Nasu, K., Shiraishi, T., Yoshioka, H., Ichinose, Y., Yamada, T. and Oku, H.

    Plant and Cell Physiology   Vol. 33 ( 5 ) page: 617-626   1992

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  104. Regulation of polyphosphoinositide metabolism in ea plasma membranes by elicitor and suppressor from a pea pathogen, Mycosphaerella pinodes. Reviewed

    Toyoda, K., Shiraishi, T., Yoshioka, H., Yamada, T., Ichinose, Y. and Oku, H.

    Plant and Cell Physiology   Vol. 33 ( 4 ) page: 445-452   1992

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  105. Preparation of the infection court by Erysiphe graminis f. sp. hordei : Cutinase is a component of the conidial exudate. Reviewed

    Pascholati, S.F., Yoshioka, H., Kunoh, H. and Nicholson, R.L.

    Physiological and Molecular Plant Pathology   Vol. 41   page: 53-59   1992

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  106. Suppression of the activation of chitinase and β-1,3-glucanase in pea epicotyls by orthonadate and a suppressor from Mycosphaerella pinodes. Reviewed

    Yoshioka, H., Shiraishi, T., Yamada, T., Nasu, K., Ichinose, Y. and Oku, H.

    Annals of the Phytopathological Society of Japan   Vol. 58 ( 3 ) page: 405-410   1992

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  107. Orthovanadate suppresses accumulation of phenylalanine ammonia-lyase mRNA and chalcone synthase mRNA in pea epicotyls induced by elicitor from Mycosphaerella pinodes. Reviewed

    Yoshioka, H., Shiraishi, T., Kawamata, S., Nasu, K., Yamada, T., Ichinose, Y. and Oku, H.

    Plant and Cell Physiology   Vol. 33 ( 2 ) page: 201-204   1992

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  108. Inhibition of ATPase activity in pea plasma membranes in situ by a suppressor from a pea pathogen, Mycosphaerella pinodes. Reviewed

    Shiraishi, T., Araki, M., Yoshioka, H., Kobayashi, I., Yamada, T., Ichinose, Y., Kunoh, H. and Oku, H.

    Plant and Cell Physiology   Vol. 32 ( 7 ) page: 1067-1075   1991

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  109. エンドウ動的遺伝子の発現機構. Invited

    山田哲治・白石友紀・一瀬勇規・奥 八郎・川又伸治・田中良和・Spirasertsak, P.・安 成才・吉岡博文・橋本忠明

    生物相互間の認識機構   Vol. 27   page: 56-65   1991

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  110. Suppression of pisatin production and ATPase activity in pea plasma membranes by orthovanadate, verapamil and a suppressor from Mycosphaerella pinodes. Reviewed

    Yoshioka, H., Shiraishi, T., Yamada, T., Ichinose, Y. and Oku, H.

    Plant and Cell Physiology   Vol. 31 ( 8 ) page: 1139-1146   1990

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  111. Preparation of the infection court by Erysiphe graminis : Degradation of the host cuticle. Reviewed

    Kunoh, H., Nicholson, R.L., Yoshioka, H., Yamaoka, N. and Kobayashi, I.

    Physiological and Molecular Plant Pathology   Vol. 36   page: 397-407   1990

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  112. Preparation of the infection court by Erysiphe graminis. II. Release of esterase enzyme from conidia in response to a contact stimulus. Reviewed

    Nicholson, R.L., Yoshioka, H., Yamaoka, N. and Kunoh, H.

    Experimental Mycology   Vol. 12   page: 336-349   1988

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  113. Preparation of the infection court by Erysiphe graminis. I. Contact-mediated changes in morphology of the conidium surface. Reviewed

    Kunoh, H., Yamaoka, N., Yoshioka, H. and Nicholson, R.L.

    Experimental Mycology   Vol. 12   page: 325-335   1988

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  114. Dynamics of plant immune MAPK activity and ROS signaling in response to invaders

    Hirofumi Yoshioka, Yuta Hino, Keiichiro Iwata, Takaya Ogawa, Miki Yoshioka, Nobuaki Ishihama, Hiroaki Adachi

    Physiological and Molecular Plant Pathology   Vol. 125   page: 102000 - 102000   2023.5

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    DOI: 10.1016/j.pmpp.2023.102000

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  115. Nab-Paclitaxel for Previously Treated Advanced Non-Small Cell Lung Cancer: Analysis of Safety and Efficacy for Patients With Renal Impairment

    Yoneshima, Y; Morita, S; Ando, M; Nakamura, A; Iwasawa, S; Yoshioka, H; Goto, Y; Takeshita, M; Harada, T; Hirano, K; Oguri, T; Kondo, M; Miura, S; Hosomi, Y; Kato, T; Kubo, T; Kishimoto, J; Yamamoto, N; Nakanishi, Y; Okamoto, I

    CLINICAL LUNG CANCER   Vol. 23 ( 7 ) page: 585 - 592   2022.11

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    DOI: 10.1016/j.cllc.2022.08.011

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  116. Development of plant activator loaded nano-particles Reviewed

    Yamaguchi Kento, Kitagawa Takahiro, Tobinaga Kyouhei, Saito Takao, Yoshioka Hirofumi, Narusaka Yoshihiro

    Japanese Journal of Pesticide Science   Vol. 47 ( 2 ) page: 65 - 68   2022.8

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    DOI: 10.1584/jpestics.w22-22

  117. Multicentre, prospective, observational study investigating the most appropriate surgical option that can prevent the recurrence of primary spontaneous pneumothorax after surgery: the PATCH study, protocol

    Kadomatsu, Y; Yoshioka, H; Shigemitsu, K; Nomata, Y; Mori, S; Hijiya, K; Motoyama, H; Ichikawa, Y; Sueyoshi, K; Okasaka, T; Miyamoto, E; Kobayashi, M; Takahashi, M; Fujinaga, T; Takechi, H; Yamagishi, H; Takuwa, T; Kobayashi, J; Sakamoto, J; Taniguchi, T; Hanaoka, N; Kubo, Y; Chen-Yoshikawa, TF

    BMJ OPEN   Vol. 11 ( 12 ) page: e052045   2021.12

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  118. Senescent cell removal via JAG1-NOTCH1 signalling in the epidermis

    Yoshioka, H; Yamada, T; Hasegawa, S; Miyachi, K; Ishii, Y; Hasebe, Y; Inoue, Y; Tanaka, H; Iwata, Y; Arima, M; Sugiura, K; Akamatsu, H

    EXPERIMENTAL DERMATOLOGY   Vol. 30 ( 9 ) page: 1268 - 1278   2021.9

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  119. Prognostic impact of geriatric assessment in elderly patients with non-small cell lung cancer: an integrated analysis of two randomized phase III trials (JCOG1115-A)

    Katayama, H; Mizusawa, J; Fukuda, H; Nakamura, S; Nakamura, K; Saijo, N; Yokoyama, A; Ohe, Y; Shinkai, T; Nakagawa, K; Abe, T; Mitsuoka, S; Okamoto, H; Yamamoto, N; Yoshioka, H; Ando, M; Tamura, T; Takeda, K

    JAPANESE JOURNAL OF CLINICAL ONCOLOGY   Vol. 51 ( 5 ) page: 685 - 692   2021.5

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  120. Oral RNAi of<i>diap1</i>results in rapid reduction of damage to potatoes in<i>Henosepilachna vigintioctopunctata</i>

    Chikami, Y; Kawaguchi, H; Suzuki, T; Yoshioka, H; Sato, Y; Yaginuma, T; Niimi, T

    JOURNAL OF PEST SCIENCE   Vol. 94 ( 2 ) page: 505 - 515   2021.3

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    DOI: 10.1007/s10340-020-01276-w

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  121. Phase III Study Comparing Nab-Paclitaxel With Docetaxel in Patients With Previously Treated Advanced Non-Small-Cell Lung Cancer

    Nakamura, A; Yoneshima, Y; Morita, S; Ando, M; Iwasawa, S; Yoshioka, H; Goto, Y; Takeshita, M; Harada, T; Hirano, K; Oguri, T; Kondo, M; Miura, S; Hosomi, Y; Kato, T; Kubo, T; Kishimoto, J; Yamamoto, N; Nakanishi, Y; Okamoto, I

    JOURNAL OF THORACIC ONCOLOGY   Vol. 16 ( 3 ) page: S107 - S108   2021.3

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  122. Role of salicylic acid glucosyltransferase in balancing growth and defence for optimum plant fitness. Reviewed International journal

    Yudai Kobayashi, Noriho Fukuzawa, Ayaka Hyodo, Hangil Kim, Shota Mashiyama, Tsuyoshi Ogihara, Hirofumi Yoshioka, Hideyuki Matsuura, Chikara Masuta, Takeshi Matsumura, Minoru Takeshita

    Molecular plant pathology   Vol. 21 ( 3 ) page: 429 - 442   2020.3

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    Salicylic acid (SA), an essential secondary messenger for plant defence responses, plays a role in maintaining a balance (trade-off) between plant growth and resistance induction, but the detailed mechanism has not been explored. Because the SA mimic benzothiadiazole (BTH) is a more stable inducer of plant defence than SA after exogenous application, we analysed expression profiles of defence genes after BTH treatment to better understand SA-mediated immune induction. Transcript levels of the salicylic acid glucosyltransferase (SAGT) gene were significantly lower in BTH-treated Nicotiana tabacum (Nt) plants than in SA-treated Nt control plants, suggesting that SAGT may play an important role in SA-related host defence responses. Treatment with BTH followed by SA suppressed SAGT transcription, indicating that the inhibitory effect of BTH is not reversible. In addition, in BTH-treated Nt and Nicotiana benthamiana (Nb) plants, an early high accumulation of SA and SA 2-O-β-d-glucoside was only transient compared to the control. This observation agreed well with the finding that SAGT-overexpressing (OE) Nb lines contained less SA and jasmonic acid (JA) than in the Nb plants. When inoculated with a virus, the OE Nb plants showed more severe symptoms and accumulated higher levels of virus, while resistance increased in SAGT-silenced (IR) Nb plants. In addition, the IR plants restricted bacterial spread to the inoculated leaves. After the BTH treatment, OE Nb plants were slightly larger than the Nb plants. These results together indicate that SAGT has a pivotal role in the balance between plant growth and SA/JA-mediated defence for optimum plant fitness.

    DOI: 10.1111/mpp.12906

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  123. 植物を元気にして病気を防ぐ―植物活力剤によるイチゴの病害抑制技術― Invited

    鳴坂義弘, 鳴坂真理, 吉岡博文, 吉岡美樹, 谷口伸治, 石川美友紀, 紀岡雄三, 野口勝憲

    植物防疫   Vol. 73 ( 11 ) page: 684-688   2019.11

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  124. Ph3 Study of Maintenance Therapy with S-1 vs BSC After Induction Therapy with Carboplatin + S-1 for Advanced Squamous Cell Lung Cancer (WJOG7512L)

    Tanaka, K; Morita, S; Ando, M; Yokoyama, T; Nakamura, A; Yoshioka, H; Ishiguro, T; Miura, S; Toyozawa, R; Oguri, T; Daga, H; Ko, R; Bessho, A; Tachihara, M; Iwamoto, Y; Hirano, K; Nakanishi, Y; Nakagawa, K; Yamamoto, N; Okamoto, I

    JOURNAL OF THORACIC ONCOLOGY   Vol. 14 ( 10 ) page: S302 - S302   2019.10

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    DOI: 10.1016/j.jtho.2019.08.605

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  125. Randomized phase 3 study of maintenance therapy with S-1 plus best supportive care (BSC) versus BSC alone after induction therapy with carboplatin plus S-1 for advanced or relapsed squamous cell lung carcinoma (WJOG7512L).

    Tanaka, K; Morita, S; Ando, M; Yokoyama, T; Nakamura, A; Yoshioka, H; Ishiguro, T; Miura, S; Toyozawa, R; Oguri, T; Daga, H; Ko, R; Bessho, A; Tachihara, M; Iwamoto, Y; Hirano, K; Nakanishi, Y; Nakagawa, K; Yamamoto, N; Okamoto, I

    JOURNAL OF CLINICAL ONCOLOGY   Vol. 37 ( 15 )   2019.5

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    DOI: 10.1200/JCO.2019.37.15_suppl.e20531

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  126. WRKYs phosphorylated by MAPK regulate chloroplast-mediated ROS burst in plant immunity

    Yoshioka, H; Adachi, H; Yoshioka, M

    PHYTOPATHOLOGY   Vol. 108 ( 10 )   2018.10

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  127. Phase Competition, Solitons, and Domain Walls in Neutral-Ionic Transition Systems

    Tsuchiizu, M; Yoshioka, H; Seo, H

    JOURNAL OF THE PHYSICAL SOCIETY OF JAPAN   Vol. 85 ( 10 )   2016.10

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    DOI: 10.7566/JPSJ.85.104705

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  128. *WRKY transcription factors phosphorylated by MAPK regulate a plant immune NADPH oxidase in <i>Nicotiana benthamiana</i>. Reviewed

    Adachi, H, Nakano, T, Miyagawa, N, Ishihama, N, Yoshioka, M, Katou, Y, Yaeno, T, Shirasu, K, Yoshioka, H

    The Plant Cell   Vol. 27 ( 9 ) page: 2645-2663.   2015.10

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  129. リン酸化反応が制御するROSバーストの分子機構 Invited Reviewed

    吉岡博文, 安達広明, 石濱伸明, 中野孝明, 白石佑太郎, 宮川典子, 野村裕也, 吉岡美樹, 浅井秀太

    日本植物病理学会報   Vol. 81   page: 1-8.   2015.2

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  130. SEC14 Phospholipid Transfer Protein Is Involved in Lipid Signaling-Mediated Plant Immune Responses in Nicotiana benthamiana Reviewed

    Akinori Kiba, Ivan Galis, Yuko Hojo, Kouhei Ohnishi, Hirofumi Yoshioka, Yasufumi Hikichi

    PLOS ONE   Vol. 9 ( 5 ) page: e98150   2014.5

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    We previously identified a gene related to the SEC14-gene phospholipid transfer protein superfamily that is induced in Nicotiana benthamiana (NbSEC14) in response to infection with Ralstonia solanacearum. We here report that NbSEC14 plays a role in plant immune responses via phospholipid-turnover. NbSEC14-silencing compromised expression of defense-related PR-4 and accumulation of jasmonic acid (JA) and its derivative JA-Ile. Transient expression of NbSEC14 induced PR-4 gene expression. Activities of diacylglycerol kinase, phospholipase C and D, and the synthesis of diacylglycerol and phosphatidic acid elicited by avirulent R. solanacearum were reduced in NbSEC14-silenced plants. Accumulation of signaling lipids and activation of diacylglycerol kinase and phospholipases were enhanced by transient expression of NbSEC14. These results suggest that the NbSEC14 protein plays a role at the interface between lipid signaling-metabolism and plant innate immune responses.

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  131. In Vivo Phosphorylation of WRKY Transcription Factor by MAPK

    Nobuaki Ishihama, Hiroaki Adachi, Miki Yoshioka, Hirofumi Yoshioka

    Methods in Molecular Biology   Vol. 1171   page: 171 - 181   2014

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    DOI: 10.1007/978-1-4939-0922-3_14

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  132. Suppression of DS1 Phosphatidic Acid Phosphatase Confirms Resistance to Ralstonia solanacearum in Nicotiana benthamiana Reviewed

    Masahito Nakano, Masahiro Nishihara, Hirofumi Yoshioka, Hirotaka Takahashi, Tatsuya Sawasaki, Kouhei Ohnishi, Yasufumi Hikichi, Akinori Kiba

    PLOS ONE   Vol. 8 ( 9 ) page: e75124.   2013.9

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    Nicotiana benthamiana is susceptible to Ralstonia solanacearum. To analyze molecular mechanisms for disease susceptibility, we screened a gene-silenced plant showing resistance to R. solanacearum, designated as DS1 (Disease suppression 1). The deduced amino acid sequence of DS1 cDNA encoded a phosphatidic acid phosphatase (PAP) 2. DS1 expression was induced by infection with a virulent strain of R. solanacearum in an hrp-gene-dependent manner. DS1 rescued growth defects of the temperature-sensitive Delta lpp1 Delta dpp1 Delta pah1 mutant yeast. Recombinant DS1 protein showed Mg2+-independent PAP activity. DS1 plants showed reduced PAP activity and increased phosphatidic acid (PA) content. After inoculation with R. solanacearum, DS1 plants showed accelerated cell death, over-accumulation of reactive oxygen species (ROS), and hyper-induction of PR-4 expression. In contrast, DS1-overexpressing tobacco plants showed reduced PA content, greater susceptibility to R. solanacearum, and reduced ROS production and PR-4 expression. The DS1 phenotype was partially compromised in the plants in which both DS1 and NbCoi1 or DS1 and NbrbohB were silenced. These results show that DS1 PAP may affect plant immune responses related to ROS and JA cascades via regulation of PA levels. Suppression of DS1 function or DS1 expression could rapidly activate plant defenses to achieve effective resistance against Ralstonia solanacearum.

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  133. A translationally controlled tumor protein negatively regulates the hypersensitive response in <i>Nicotiana benthamiana</i>. Reviewed

    Gupta, M, Yoshioka, H, Ohnishi, K, Mizumoto, H, Hikichi, Y, Kiba, A

    Plant and Cell Physiology   Vol. 54 ( 8 ) page: 1403-1414.   2013.8

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  134. タンパク質のリン酸化によるROSバーストの分子機構 Invited

    吉岡博文, 安達広明, 石濱伸明, 中野孝明, 白石佑太郎, 宮川典子, 野村裕也, 吉岡美樹, 浅井秀太

    植物−微生物相互作用とビオトロフ感染   Vol. 48   page: 105-114.   2013.8

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  135. Ethylene responsive AP2/ERF transcription factor MACD1 participates in phytotoxin-triggered programmed cell death. Reviewed

    Mase, K, Ishihama, N, Mori, H, Takahashi, H, Kaminaka, H, Kodama, M, Yoshioka, H

    Molecular Plant-Microbe Interactions   Vol. 26   page: 868-879.   2013.7

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  136. *The variable domain of a plant calcium-dependent protein kinase (CDPK) confers subcellular localization and substrate recognition for NADPH oxidase. Reviewed

    Asai, S, Ichikawa, T, Nomura, H, Kobayashi, M, Kamiyoshihara, Y, Mori, H, Kadota, Y, Zipfel, C, Jones, J.D.G, Yoshioka, H

    The Journal of Biological Chemistry   Vol. 288   page: 14332-14340.   2013.5

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  137. The Variable Domain of a Plant Calcium-dependent Protein Kinase (CDPK) Confers Subcellular Localization and Substrate Recognition for NADPH Oxidase

    Asai, S; Ichikawa, T; Nomura, H; Kobayashi, M; Kamiyoshihara, Y; Mori, H; Kadota, Y; Zipfel, C; Jones, JDG; Yoshioka, H

    JOURNAL OF BIOLOGICAL CHEMISTRY   Vol. 288 ( 20 ) page: 14332 - 14340   2013.5

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    DOI: 10.1074/jbc.M112.448910

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  138. Nitric Oxide as a Mediator for Defense Responses

    Bellin, D; Asai, S; Delledonne, M; Yoshioka, H

    MOLECULAR PLANT-MICROBE INTERACTIONS   Vol. 26 ( 3 ) page: 271 - 277   2013.3

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    DOI: 10.1094/MPMI-09-12-0214-CR

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  139. *StCDPK5 confers resistance to late blight pathogen but increases susceptibility to early blight pathogen in potato via reactive oxygen species burst. Reviewed

    Kobayashi, M, Yoshioka, M, Asai, S, Nomura, H, Kuchimura, K, Mori, H, Doke, N, Yoshioka, H

    New Phytologist   Vol. 196   page: 223–237.   2012.10

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  140. StCDPK5 confers resistance to late blight pathogen but increases susceptibility to early blight pathogen in potato via reactive oxygen species burst

    Kobayashi, M; Yoshioka, M; Asai, S; Nomura, H; Kuchimura, K; Mori, H; Doke, N; Yoshioka, H

    NEW PHYTOLOGIST   Vol. 196 ( 1 ) page: 223 - 237   2012.10

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    DOI: 10.1111/j.1469-8137.2012.04226.x

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  141. Ethylene Signaling Pathway and MAPK Cascades Are Required for AAL Toxin-Induced Programmed Cell Death

    Mase, K; Mizuno, T; Ishihama, N; Fujii, T; Mori, H; Kodama, M; Yoshioka, H

    MOLECULAR PLANT-MICROBE INTERACTIONS   Vol. 25 ( 8 ) page: 1015 - 1025   2012.8

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    DOI: 10.1094/MPMI-02-12-0036-R

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  142. Post-translational regulation of WRKY transcription factors in plant immunity Invited Reviewed

    Nobuaki Ishihama, Hirofumi Yoshioka

    CURRENT OPINION IN PLANT BIOLOGY   Vol. 15 ( 4 ) page: 431 - 437   2012.8

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    Plants have evolved immune system to protect themselves against invading pathogens. Recent research has illustrated that signaling networks, after perception of diverse pathogen-derived signals, facilitate transcriptional reprogramming through mitogen-activated protein kinase (MAPK) cascades. WRKY proteins, which comprise a large family of plant transcription factors, are key players in plant immune responses. WRKY transcription factors participate in the control of defense-related genes either as positive or as negative regulators, and essentially are regulated at the transcriptional level. Emerging evidence emphasizes that group I WRKY transcription factors, which contain a conserved motif in the N-terminal region, are also activated by MAPK-dependent phosphorylation, underlining their importance in plant immunity.

    DOI: 10.1016/j.pbi.2012.02.003

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  143. Cell Death of <i>Nicotiana benthamiana</i> Is Induced by Secreted Protein NIS1 of <i>Colletotrichum orbiculare</i> and Is Suppressed by a Homologue of CgDN3

    Yoshino, K; Irieda, H; Sugimoto, F; Yoshioka, H; Okuno, T; Takano, Y

    MOLECULAR PLANT-MICROBE INTERACTIONS   Vol. 25 ( 5 ) page: 625 - 636   2012.5

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    DOI: 10.1094/MPMI-12-11-0316

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  144. Observation of Riemannian geometric effects on electronic states

    Onoe, J; Ito, T; Shima, H; Yoshioka, H; Kimura, S

    EPL   Vol. 98 ( 2 )   2012.4

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    DOI: 10.1209/0295-5075/98/27001

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  145. Isolation and characterization of an asparagine-rich protein that regulates hypersensitive cell death-mediated resistance in Nicotiana plants Reviewed

    Daisuke Komori, Masahiro Nishihara, Akiko Takahashi, Meenu Gupta, Hirofumi Yoshioka, Hiroyuki Mizumoto, Kouhei Ohnishi, Yusufumi Hikichi, Akinori Kiba

    PLANT BIOTECHNOLOGY   Vol. 29 ( 3 ) page: 293 - 300   2012

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    We isolated and characterized Ralstonia solanacearum-responsive genes (RsRGs) related to the hypersensitive response (HR) by virus-induced gene silencing using Nicotiana benthamiana and the potato virus x vector system. We focused on RsRGM10, because induction of HR was delayed in RsRGM10-silenced plants challenged with incompatible R. solanacearum 8107, which induces the HR in, N. benthamiana. The amino acid sequence deduced from the full-length RsRGM10 cDNA showed approximately 90% identity with rolB overexpressed 1 (ROX1) from N. tabacum, and contained an asparagine-rich sequence. We designated this protein NbARP (N. benthamiana asparagine-rich protein). Expression of HR-related hin1 and onset of the oxidative burst were delayed in NbARP-silenced plants, and induction of myelin basic kinase activity was reduced. Growth of R. solanacearum 8107 was accelerated in NbARP-silenced plants. Induction of the HR by Pseudomonas cichorii and Pseudomonas syringae pv. syringae was also delayed in NbARP-silenced plants. Silencing of NbARP reduced induction of HR-cell death by Agrobacterium tumefaciens-mediated transient expression of HR elicitors and a constitutively active form of mitogen activated protein kinase kinase. The population of R. solanacearum 8107 was decreased in NbARP-overexpressing plants. These results suggest that NbARP is closely related to the HR.

    DOI: 10.5511/plantbiotechnology.12.0213b

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  146. SHAPE OPTIMIZATION OF THERMOELASTIC FIELDS FOR STIFFNESS MAXIMIZATION

    Katamine, E; Yoshioka, H; Azegami, H

    PROCEEDINGS OF THE ASME INTERNATIONAL DESIGN ENGINEERING TECHNICAL CONFERENCES AND COMPUTERS AND INFORMATION IN ENGINEERING CONFERENCE 2012, VOL 2, PTS A AND B     page: 853 - +   2012

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  147. NINJAはコリプレッサーTOPLESSとジャスモン酸シグナルをつなぐ―植物ホルモンの受容による新しい転写制御機構の役割は? Invited

    野村裕也, 吉岡博文

    化学と生物   Vol. 49   page: 85-87   2011.2

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  148. Nitric oxide and reactive oxygen species in plant defense responses Invited

    Yoshioka H, Asai S, Yoshioka M, Kobayashi M, Doke N

    In Genome-Enabled Integration of Research in Plant Pathogen Systems. (Wolpert, T., Shiraishi, T., Allen, C., Glazebrook, J. and Akimitsu, K. eds), APS Press     page: 47 - 55   2011

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  149. Analyses of the cis-Regulatory Regions Responsible for the Transcriptional Activation of the N Resistance Gene by Tobacco mosaic virus Reviewed

    Michie Kobayashi, Nobuaki Ishihama, Hirofumi Yoshioka, Reona Takabatake, Shinya Tsuda, Shigemi Seo, Yuko Ohashi, Ichiro Mitsuhara

    JOURNAL OF PHYTOPATHOLOGY   Vol. 158 ( 11-12 ) page: 826 - 828   2010.12

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    Although the tobacco N gene, which confers resistance to Tobacco mosaic virus (TMV), is transcriptionally induced by infection with TMV, the regulatory mechanism has not previously been elucidated. We found that a 5&apos;-flanking region of the N gene exhibits TMV-inducible promoter activity and that the region from -290 to -271 includes a cis-acting element in response to infection. The activity required N protein and signal components of N-mediated resistance. These results suggest that transcription of the N gene is positively regulated by N-mediated resistance signalling.

    DOI: 10.1111/j.1439-0434.2010.01686.x

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  150. Regulation of Arabidopsis defense responses against Spodoptera littoralis by CPK-mediated calcium signaling Reviewed

    Chidananda Nagamangala Kanchiswamy, Hirotaka Takahashi, Stefano Quadro, Massimo E. Maffei, Simone Bossi, Cinzia Bertea, Simon Atsbaha Zebelo, Atsushi Muroi, Nobuaki Ishihama, Hirofumi Yoshioka, Wilhelm Boland, Junji Takabayashi, Yaeta Endo, Tatsuya Sawasaki, Gen-ichiro Arimura

    BMC PLANT BIOLOGY   Vol. 10   page: 97   2010.5

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    Background: Plant Ca(2+) signals are involved in a wide array of intracellular signalling pathways after pest invasion. Ca(2+)-binding sensory proteins such as Ca(2+)-dependent protein kinases (CPKs) have been predicted to mediate the signaling following Ca(2+) influx after insect herbivory. However, until now this prediction was not testable.
    Results: To investigate the roles CPKs play in a herbivore response-signaling pathway, we screened the characteristics of Arabidopsis CPK mutants damaged by a feeding generalist herbivore, Spodoptera littoralis. Following insect attack, the cpk3 and cpk13 mutants showed lower transcript levels of plant defensin gene PDF1.2 compared to wild-type plants. The CPK cascade was not directly linked to the herbivory-induced signaling pathways that were mediated by defense-related phytohormones such as jasmonic acid and ethylene. CPK3 was also suggested to be involved in a negative feedback regulation of the cytosolic Ca(2+) levels after herbivory and wounding damage. In vitro kinase assays of CPK3 protein with a suite of substrates demonstrated that the protein phosphorylates transcription factors (including ERF1, HsfB2a and CZF1/ZFAR1) in the presence of Ca(2+). CPK13 strongly phosphorylated only HsfB2a, irrespective of the presence of Ca(2+). Furthermore, in vivo agroinfiltration assays showed that CPK3-or CPK13-derived phosphorylation of a heat shock factor (HsfB2a) promotes PDF1.2 transcriptional activation in the defense response.
    Conclusions: These results reveal the involvement of two Arabidopsis CPKs (CPK3 and CPK13) in the herbivory-induced signaling network via HsfB2a-mediated regulation of the defense-related transcriptional machinery. This cascade is not involved in the phytohormone-related signaling pathways, but rather directly impacts transcription factors for defense responses.

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  151. S-Glycoprotein-Like Protein Regulates Defense Responses in Nicotiana Plants against Ralstonia solanacearum Reviewed

    Milimo Maimbo, Kouhei Ohnishi, Yasufumi Hikichi, Hirofumi Yoshioka, Akinori Kiba

    PLANT PHYSIOLOGY   Vol. 152 ( 4 ) page: 2023 - 2035   2010.4

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    RsRGA4 (for Ralstonia solanacearum-responsive gene A4) encodes a polypeptide similar to S-locus glycoprotein (SGP) from Brassica rapa and SGP-like proteins from Ipomoea trifida and Medicago truncatula. Therefore, we designated RsRGA4 as NtSGLP (for Nicotiana tabacum SGP-like protein) and NbSGLP (its Nicotiana benthamiana ortholog). NbSGLP is expressed in root, leaf, petal, gynoecium, and stamen. Expression of NbSGLP was strongly induced by inoculation with an avirulent strain of R. solanacearum (Rs8107) and slightly enhanced by inoculation with virulent R. solanacearum (RsOE1-1). Expression of NbSGLP was induced by inoculation with an hrpY-deficient mutant of RsOE1-1 and Rs8107. Expression was also induced by aminocyclopropane carboxylic acid and salicylic acid. Virus-induced gene silencing of NbSGLP enhanced the growth of Rs8107. Growth of RsOE1-1 and appearance of wilt symptoms were also accelerated in silenced plants. Expression of PR-1a and EREBP was reduced, and markers for basal defense, such as callose deposition and reduced vascular flow, were compromised in NbSGLP-silenced plants. Moreover, growth of Pseudomonas cichorii, Pseudomonas syringae pv tabaci, and P. syringae pv mellea was also enhanced in the silenced plants. On the other hand, silencing of NbSGLP did not interfere with the appearance of the hypersensitive response. NbSGLP was secreted in a signal peptide-dependent manner. Agrobacterium tumefaciens-mediated expression of NbSGLP induced PR-1a and EREBP expression, callose deposition, and reduced vascular flow. NbSGLP-induced callose deposition and reduced vascular flow were not observed in salicylic acid-deficient N. benthamiana NahG plants. Taken together, SGLP might have a role in the induction of basal defense in Nicotiana plants.

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  152. Ca2+-dependent protein kinases and their substrate HsfB2a are differently involved in the heat response signaling pathway in Arabidopsis Reviewed

    Chidananda Nagamangala Kanchiswamy, Atsushi Muroi, Massimo E. Maffei, Hirofumi Yoshioka, Tatsuya Sawasaki, Gen-ichiro Arimura

    PLANT BIOTECHNOLOGY   Vol. 27 ( 5 ) page: 469 - 473   2010

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    Little is known about the mechanisms by which Ca2+-binding sensory proteins direct the plant heat shock (HS) response. Since two Ca2+-dependent protein kinases (CPK3 and CPK13) were recently shown to phosphorylate the heat shock transcription factor HsfB2a, we assessed in the current study whether these kinases are also involved in HS signal transduction, by monitoring the transcriptional profile of HS protein (Hsp) family genes in Arabidopsis Col-0 plants (WT) and the corresponding mutants. Both with and without HS, the gene transcript levels of Hsp70, Hsp101, Hsp17.4-CIII and Hsp15.7-CI were found to be lower in cpk3 and cpk13 mutants compared to WT, resulting in the impairment of basal thermotolerance in the mutants. To determine the in vivo function of CPKs, CPK3/13 and their substrate HsfB2a (heat shock transcription factor) were co-expressed as cofactors for the transient expression of a reporter (GUS) gene under the control of heat shock element (HSE) in Nicotiana benthamiana leaves. However, CPK3/13-phosphorylated HsfB2a did not function in the suppression/activation of HSE-promoted expression in the transient expression system. Implications for possible signal trafficking via CPKs and Hsfs are discussed.

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  153. Remotely operable compact instruments for measuring atmospheric CO<sub>2</sub> and CH<sub>4</sub> column densities at surface monitoring sites

    Kobayashi, N; Inoue, G; Kawasaki, M; Yoshioka, H; Minomura, M; Murata, I; Nagahama, T; Matsumi, Y; Tanaka, T; Morino, I; Ibuki, T

    ATMOSPHERIC MEASUREMENT TECHNIQUES   Vol. 3 ( 4 ) page: 1103 - 1112   2010

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  154. Role of nitric oxide and reactive oxygen species in disease resistance to necrotrophic pathogens. Invited Reviewed

    Asai, S, Mase, K, Yoshioka, H

    Plant Signaling and Behavior   Vol. 5   page: 872-874   2010

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  155. Riboflavin synthesis participates in plant immune responses in Nicotiana benthamiana Invited

    Asai S, Mase K, Yoshioka H

    In Biology of Plant-Microbe Interactions Vol. 7. Chapter 28, Proceedings of the 14th International Congress on Molecular Plant-Microbe Interactions. (Antoun, H., Avis, T., Brisson, L., Prevost, D. and Trepanier, M. eds), IS-MPMI Press, MN     2010

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  156. 病害抵抗性・感受性決定に関わる植物の感染応答機構 Invited

    木場章範, 中野真人, 伊藤 慎, Maimbo, M, 大西浩平, 吉岡博文, 水本祐之, 曵地康史

    農業現場の問題解決に向けた感染生理学   Vol. 46   page: 1-8   2010

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  157. Molecular mechanisms of generation for nitric oxide and reactive oxygen species, and role of the radical burst in plant immunity Invited Reviewed

    Hirofumi Yoshioka, Shuta Asai, Miki Yoshioka, Michie Kobayashi

    MOLECULES AND CELLS   Vol. 28 ( 4 ) page: 321 - 329   2009.10

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    Rapid production of nitric oxide (NO) and reactive oxygen species (ROS) has been implicated in the regulation of innate immunity in plants. A potato calcium-dependent protein kinase (StCDPK5) activates an NADPH oxidase StRBOHA to D by direct phosphorylation of N-terminal regions, and heterologous expression of StCDPK5 and StRBOHs in Nicotiana benthamiana results in oxidative burst. The transgenic potato plants that carry a constitutively active StCDPK5 driven by a pathogen-inducible promoter of the potato showed high resistance to late blight pathogen Phytophthora infestans accompanied by HR-like cell death and H(2)O(2) accumulation in the attacked cells. In contrast, these plants showed high susceptibility to early blight necrotrophic pathogen Alternaria solani, suggesting that oxidative burst confers high resistance to biotrophic pathogen, but high susceptibility to necrotrophic pathogen. NO and ROS synergistically function in defense responses. Two MAPK cascades, MEK2-SIPK and cytokinesis-related MEK1-NTF6, are involved in the induction of NbRBOHB gene in N. benthamiana. On the other hand, NO burst is regulated by the MEK2-SIPK cascade. Conditional activation of SIPK in potato plants induces oxidative and NO bursts, and confers resistance to both biotrophic and necrotrophic pathogens, indicating the plants may have obtained during evolution the signaling pathway which regulates both NO and ROS production to adapt to wide-spectrum pathogens.

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  158. INF1 Elicitin Activates Jasmonic Acid- and Ethylene-mediated Signalling Pathways and Induces Resistance to Bacterial Wilt Disease in Tomato Reviewed

    Yoko Kawamura, Shu Hase, Shigehito Takenaka, Yoshinori Kanayama, Hirofumi Yoshioka, Sophien Kamoun, Hideki Takahashi

    JOURNAL OF PHYTOPATHOLOGY   Vol. 157 ( 5 ) page: 287 - 297   2009.5

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    Phytophthora infestans, the cause of potato and tomato late blight disease, produces INF1 elicitin, a 10 kDa extracellular protein. INF1 induces a hypersensitive response (HR) and systemic acquired resistance in species of the Nicotiana genus and a few other genera. We analysed the response of tomato to INF1 and INF1S3, which has a Cys to Ser substitution at position 3 of the processed protein and therefore lacks HR induction activity in tobacco. No HR cell death was induced in either INF1- or INF1S3-treated tomato leaves. The expression of salicylic acid (SA)-responsive PR-1a(P6) and PR-2a genes was not induced by treatment with either INF1 or INF1S3. However, the expression of jasmonic acid (JA)-responsive PR-6 encoding proteinase inhibitor II, LeATL6 encoding ubiquitin ligase E3, and LOX-E encoding lipoxygenase, was up-regulated in tomato leaves treated with INF1 but not in those treated with INF1S3. Their induction was completely compromised in INF1-treated jai1-1 mutant tomato, in which the JA signalling pathway is impaired. The accumulation of ethylene (ET) and the expression of ET-responsive genes were also induced in tomato by INF1 but not INF1S3 treatment. The activation of JA and ET-mediated signals but not the SA-mediated signalling in INF1-treated tomato was also demonstrated by global gene expression analysis. INF1-treated tomatoes, but not those treated with INF1S3, exhibited resistance to bacterial wilt disease caused by Ralstonia solanacearum. Thus, INF1 seems to induce resistance to bacterial wilt disease in tomato and activate JA- and ET-mediated signalling pathways without development of HR cell death.

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  159. Molecular mechanisms of the radical burst in plant immunity Invited

    Yoshioka H, Asai S, Miyagawa N, Ichikawa T, Yoshioka M, Kobayashi M

    In Molecular Plant-Microbe Interactions. (Bouarab, K., Brisson, N. and Daayf, F. eds), CAB International, Oxfordshire, UK     page: 59 - 74   2009

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  160. Nitric oxide as a partner of reactive oxygen species participates in disease resistance to necrotrophic pathogen <i>Botrytis cinerea</i> in <i>Nicotiana benthamiana</i>. Reviewed

    Asai, S, Yoshioka, H

    Molecular Plant-Microbe Interactions   Vol. 22   page: 619-629   2009

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  161. Molecular Mechanisms of the Radical Burst in Plant Immunity

    Yoshioka, H; Asai, S; Miyagawa, N; Ichikawa, T; Yoshioka, M; Kobayashi, M

    MOLECULAR PLANT-MICROBE INTERACTIONS     page: 59 - 74   2009

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    DOI: 10.1079/9781845935740.0059

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  162. MAPK signaling regulates nitric oxide and NADPH oxidase-dependent oxidative bursts in <i>Nicotiana benthamiana</i>

    Asai, S; Ohta, K; Yoshioka, H

    PLANT CELL   Vol. 20 ( 5 ) page: 1390 - 1406   2008.5

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  163. Involvement of <i>NbNOA1</i> in NO production and defense responses in INF1-treated <i>Nicotiana benthamiana</i>

    Kato, H; Asai, S; Yamamoto-Katou, A; Yoshioka, H; Doke, N; Kawakita, K

    JOURNAL OF GENERAL PLANT PATHOLOGY   Vol. 74 ( 1 ) page: 15 - 23   2008.2

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    DOI: 10.1007/s10327-007-0054-4

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  164. *MAPK signaling regulates nitric oxide and NADPH oxidase-dependent oxidative bursts in <i>Nicotiana benthamiana</i>. Reviewed

    Asai, S, Ohta, K, Yoshioka, H

    The Plant Cell   Vol. 20   page: 1390-1406   2008

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  165. Discovery of oxidative burst in the field of plant immunity: Looking back at the early pioneering works and towards the future development. Reviewed

    Yoshioka, H, Bouteau, F, Kawano, T

    Plant Signaling and Behavior   Vol. 3   page: 153-155   2008

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  166. The role of radical burst via MAPK signaling in plant immunity. Invited Reviewed

    Asai, S, Yoshioka, H

    Plant Signaling and Behavior   Vol. 3   page: 920-922   2008

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  167. Induction of a small heat shock protein and its functional roles in Nicotiana plants in the defense response against Ralstonia solanacearum(1[W]) Reviewed

    Milimo Maimbo, Kouhei Ohnishi, Yasufumi Hikichi, Hirofumi Yoshioka, Akinori Kiba

    PLANT PHYSIOLOGY   Vol. 145 ( 4 ) page: 1588 - 1599   2007.12

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    In tobacco (Nicotiana tabacum), Ralstonia solanacearum OE1-1 (RsOE1-1) is pathogenic, whereas R. solanacearum 8107 (Rs8107) is nonpathogenic and induces the hypersensitive response (HR). To elucidate the molecular mechanisms of plant-R. solanacearum interactions, we used differential display to isolate a cDNA fragment, A6, regulated in tobacco by inoculation with RsOE1-1. The deduced amino acid sequence predicted from full-length A6-cDNA showed similarity to small heat shock proteins from Arabidopsis (Arabidopsis thaliana; hypothetical protein), Medicago truncatula, and Cucumis melo; we therefore designated A6 to correspond to Ntshsp17 (for tobacco small heat shock protein 17). Recombinant Ntshsp17 overproduced in Escherichia coli exhibited molecular chaperone function. Expression of Ntshsp17 was increased in tobacco leaves inoculated with both RsOE1-1 and Rs8107. Expression was induced by heat treatment and by treatment with aminocyclopropane carboxylic acid, hydrogen peroxide, methyl jasmonate, and salicylic acid. Ntshsp17 expression was induced by inoculation with a HR and pathogenicity gene mutant of Rs8107 that does not induce the HR, but not by Agrobacterium-mediated transient expression of INF1, an HR elicitor. In Nbshsp17-silenced plants (an Ntshsp17 ortholog in Nicotiana benthamiana), expression of ETHYLENE-RESPONSE ELEMENT-BINDING PROTEIN, PATHOGENESIS-RELATED1a (PR1a), and PR4 genes was compromised, but expression of ELONGATION FACTOR1 alpha was scarcely affected. Appearance of the HR was not affected in the silenced plants. In the silenced plants, growth of Rs8107 was accelerated. Bacterial growth and wilt symptoms elicited by RsOE1-1 were also accelerated in the silenced plants. These results indicate that this small heat shock protein might have a role in HR-independent defenses in Nicotiana plants.

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  168. Regulation of rice NADPH oxidase by binding of Rac GTPase to its N-terminal extension Reviewed

    Hann Ling Wong, Reinhard Pinontoan, Kokoro Hayashi, Ryo Tabata, Takashi Yaeno, Kana Hasegawa, Chojiro Kojima, Hirofumi Yoshioka, Koh Iba, Tsutomu Kawasaki, Ko Shimamoto

    PLANT CELL   Vol. 19 ( 12 ) page: 4022 - 4034   2007.12

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    Reactive oxygen species (ROS) produced by NADPH oxidase play critical roles in various cellular activities, including plant innate immunity response. In contrast with the large multiprotein NADPH oxidase complex of phagocytes, in plants, only the homologs of the catalytic subunit gp91(phox) and the cytosolic regulator small GTPase Rac are found. Plant homologs of the gp91phox subunit are known as Rboh (for respiratory burst oxidase homolog). Although numerous Rboh have been isolated in plants, the regulation of enzymatic activity remains unknown. All rboh genes identified to date possess a conserved N-terminal extension that contains two Ca2+ binding EF-hand motifs. Previously, we ascertained that a small GTPase Rac (Os Rac1) enhanced pathogen-associated molecular pattern-induced ROS production and resistance to pathogens in rice (Oryza sativa). In this study, using yeast two-hybrid assay, we found that interaction between Rac GTPases and the N-terminal extension is ubiquitous and that a substantial part of the N-terminal region of Rboh, including the two EF-hand motifs, is required for the interaction. The direct Rac-Rboh interaction was supported by further studies using in vitro pull-down assay, a nuclear magnetic resonance titration experiment, and in vivo fluorescence resonance energy transfer (FRET) microscopy. The FRET analysis also suggests that cytosolic Ca2+ concentration may regulate Rac-Rboh interaction in a dynamic manner. Furthermore, transient coexpression of Os Rac1 and rbohB enhanced ROS production in Nicotiana benthamiana, suggesting that direct Rac-Rboh interaction may activate NADPH oxidase activity in plants. Taken together, the results suggest that cytosolic Ca2+ concentration may modulate NADPH oxidase activity by regulating the interaction between Rac GTPase and Rboh.

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  169. Involvement of mitogen-activated protein kinase in the induction of StrbohC and StrbohD genes in response to pathogen signals in potato Reviewed

    Chihiro Yamamizo, Noriyuki Doke, Hirofumi Yoshioka, Kazuhito Kawakita

    Journal of General Plant Pathology   Vol. 73 ( 5 ) page: 304 - 313   2007.10

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    The respiratory burst oxidase homolog (rboh), a plant homolog of the catalytic subunit of phagocyte NADPH oxidase (gp91phox ), has been implicated in O2- generation. In the previous study, we reported that StrbohA is constitutively expressed at a low level, whereas StrbohB is upregulated after pathogen signals are perceived in potato tubers. To gain a better understanding about the role of rboh in the defense response in potato leaves, we investigated the regulatory mechanisms of the gene. StrbohB gene was activated in response to an avirulent race of Phytophthora infestans or to hyphal wall components (HWC) from the pathogen
    however, levels of expression were very low. We isolated two novel rboh cDNAs, StrbohC and StrbohD, by virtue of the sequence information from the tomato whitefly-induced gene 1 (LeWfi1) and tomato TC121349, respectively. The expression of StrbohC and StrbohD was drastically upregulated in the potato leaves inoculated with P. infestans or treated with HWC elicitor. Mitogen-activated protein kinase (MAPK) is known to play a pivotal role in the oxidative burst. The transcript accumulation induced by HWC treatment was blocked by U0126, which is a specific inhibitor of MAPK kinase (MAPKK). Conversely, the StrbohC and StrbohD genes were upregulated, driven by a pathogen-inducible promoter, in transgenic potato leaves that carry a constitutively active mutant of the MAPKK, StMEK1 DD, that activates defense-related MAPKs. These results suggest that a MAPK cascade is involved in the process leading to the expression of the StrbohC and StrbohD genes. © 2007 The Phytopathological Society of Japan and Springer.

    DOI: 10.1007/s10327-007-0020-1

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  170. Calcium-dependent protein kinases regulate the production of reactive oxygen species by potato NADPH oxidase Reviewed

    Michie Kobayashi, Ikuko Ohura, Kazuhito Kawakita, Naohiko Yokota, Masayuki Fujiwara, Ko Shimamoto, Noriyuki Doke, Hirofumi Yoshioka

    PLANT CELL   Vol. 19 ( 3 ) page: 1065 - 1080   2007.3

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    Reactive oxygen species (ROS) are implicated in plant innate immunity. NADPH oxidase (RBOH; for Respiratory Burst Oxidase Homolog) plays a central role in the oxidative burst, and EF-hand motifs in the N terminus of this protein suggest possible regulation by Ca2+. However, regulatory mechanisms are largely unknown. We identified Ser-82 and Ser-97 in the N terminus of potato (Solanum tuberosum) St RBOHB as potential phosphorylation sites. An anti-phosphopeptide antibody (pSer82) indicated that Ser-82 was phosphorylated by pathogen signals in planta. We cloned two potato calcium-dependent protein kinases, St CDPK4 and St CDPK5, and mass spectrometry analyses showed that these CDPKs phosphorylated only Ser-82 and Ser-97 in the N terminus of St RBOHB in a calcium-dependent manner. Ectopic expression of the constitutively active mutant of St CDPK5, St CDPK5VK, provoked ROS production in Nicotiana benthamiana leaves. The CDPK-mediated ROS production was disrupted by knockdown of Nb RBOHB in N. benthamiana. The loss of function was complemented by heterologous expression of wild-type potato St RBOHB but not by a mutant (S82A/S97A). Furthermore, the heterologous expression of St CDPK5VK phosphorylated Ser-82 of St RBOHB in N. benthamiana. These results suggest that St CDPK5 induces the phosphorylation of St RBOHB and regulates the oxidative burst.

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  171. Non-destructive method to detect the cycle of lamination in sedimentary rocks: rhythmite sequence in Neoproterozoic Cap carbonates

    Katsuta, N; Tojo, B; Takano, M; Yoshioka, H; Kawakami, S; Ohno, T; Kumazawa, M

    RISE AND FALL OF THE EDIACARAN BIOTA   Vol. 286   page: 27 - 34   2007

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    DOI: 10.1144/SP286.3

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  172. 植物免疫におけるMAPキナーゼとラジカルバースト.植物における環境と生物ストレスに対する応答 Invited

    吉岡博文, 浅井秀太

    蛋白質核酸酵素 増刊号   Vol. 52   page: 667-672   2007

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  173. 植物病原細菌の感染を制御する宿主応答機構~ <i>Ralstonia solanacearum- Nicotiana</i>属植物相互作用の解析~ Invited

    木場章範, Maimbo, M, 小森大輔, 張 宛果, 富山裕美, 高橋彰子, 大西浩平, 吉岡博文, 曳地康史

    植物-病原微生物の相互作用のダイナミズム   Vol. 43   page: 55-65   2007

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  174. 植物免疫におけるラジカルバーストの分子機構 Invited

    吉岡博文, 浅井秀太, 間瀬圭介, 市川達士, 小林光智衣, 小八重善裕

    植物-病原微生物の相互作用のダイナミズム   Vol. 43   page: 37-47   2007

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  175. Nitrate reductase is responsible for elicitin-induced nitric oxide production in nicotiana benthamiana Reviewed

    Ayako Yamamoto-Katou, Shinpei Katou, Hirofumi Yoshioka, Noriyuki Doke, Kazuhito Kawakita

    PLANT AND CELL PHYSIOLOGY   Vol. 47 ( 6 ) page: 726 - 735   2006.6

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    Recent works have established a key role for nitric oxide (NO) in activating disease resistance in plants. Nitrate reductase (NR) is one of the enzymes that are capable of producing NO in plants. In a previous study, we reported that pathogen signals induce expression of NR genes in potato, suggesting the involvement of NR in NO production induced by pathogen signals. In this study, we cloned NR genes from Nicotiana benthamiana and investigated their involvement in NO production induced by INF1, a major elicitin secreted by Phytophthora infestans. Treatment of protoplasts prepared from N. benthamiana leaves with INF1 elevated NO production to a maximum level 1-3 h after treatment. INF1-induced NO generation was suppressed completely by an NO-specific scavenger, but partially by a nitric oxide synthase inhibitor. To investigate the involvement of NR in INF1-induced NO production, NR genes were silenced by virus-induced gene silencing. The NR-silenced plants showed yellowish leaves which resemble the characteristic of Arabidopsis NR double mutants. Silencing of NR genes significantly decreased both NO2--producing activity and INF1-induced NO production, indicating that NR is involved in INF1-induced NO production. In contrast, overexpression of NbNR1 encoding N. benthamiana NR by Agrobacterium-mediated transient expression elevated NO2--producing activity nine times over the control; however, INF1-induced NO production in protoplasts overexpressing NbNR1 was comparable with that in control protoplasts. These results suggest that NR is involved in INF1-induced NO production, and post-translational modification of NR or availability of substrate NO2- may be a rate-limiting step of NO production by NR.

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  176. Peroxynitrite generation and tyrosine nitration in defense responses in tobacco BY-2 cells

    Syuhei Saito, Ayako Yamamoto-Katou, Hirofumi Yoshioka, Noriyuki Doke, Kazuhito Kawakita

    PLANT AND CELL PHYSIOLOGY   Vol. 47 ( 6 ) page: 689 - 697   2006.6

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    Peroxynitrite (ONOO-) is a compound formed by reaction of superoxide (O-2 (-)) with nitric oxide (NO) and is expected to possess characteristics of both O-2 (-) reactivity and NO mobility in order to function as a signal molecule. Although there are several reports that describe the role of ONOO- in defense responses in plants, it has been very difficult to detect ONOO- in bioimaging due to its short half-life or paucity of methods for ONOO--specific detection among reactive oxygen species or free radicals. Aminophenyl fluorescein (APF), a recently developed novel fluorophore for direct detection of ONOO- in bioimaging, was used for intracellular ONOO- detection. ONOO- generation in tobacco BY-2 cells treated with INF1, the major elicitin secreted by the late blight pathogen Phytophthora infestans, occurred within 1 h and reached a maximum level at 6-12 h after INF1 treatment. Urate, a ONOO- scavenger, abolished INF1-induced ONOO- generation. It is well known that ONOO- reacts with tyrosine residues in proteins to form nitrotyrosine in a nitration reaction as an ONOO--specific reaction. Western blot analysis using anti-nitrotyrosine antibodies recognized nitrotyrosine-containing proteins in 20 and 50 kDa bands in BY-2 protein extract containing SIN-1 [3-(4-morpholinyl) sydnonimine hydrochloride; an ONOO- donor]. These bands were also recognized in INF1-treated BY-2 cells and were found to be slightly suppressed by urate. Our study is the first to report ONOO- detection and tyrosine nitration in defense responses in plants.

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  177. Loss of AtPDR8, a plasma membrane ABC transporter of Arabidopsis thaliana, causes hypersensitive cell death upon pathogen infection Reviewed

    Y Kobae, T Sekino, H Yoshioka, T Nakagawa, E Martinoia, M Maeshima

    PLANT AND CELL PHYSIOLOGY   Vol. 47 ( 3 ) page: 309 - 318   2006.3

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    Plants contain a large number of ATP-binding cassette (ABC) transporters belonging to different subclasses. AtPDR8 is the only member of the pleiotropic drug resistance (PDR) ABC transporter subclass in Arabidopsis that is constitutively highly expressed. In transgenic Arabidopsis plants harboring the AtPDR8 promoter fused to beta-glucuronidase (GUS), reporter expression was shown to he strong in the stomata and hydathode. In the stomata, transcripts of AtPDR8 were particularly frequent in the cells surrounding air spaces. Subcellular fractionation and immunochemical analysis showed that AtPDR8 was localized in the plasma membrane. When a knockout mutant of AtPDR8 (atpdr8) was infected with bacterial and oomycete pathogens, the plants exhibited chlorotic lesions and a hypersensitive response (HR)-like cell death. Cell death was detected in the atpdr8 mutants within 10 h of infection with the virulent bacterial pathogen, Pseudomonas syringae. As a result, the growth of P syringae in the leaves of the atpdr8 mutant was reduced to 1% of that in the wild type. The defense response genes, PR-1, PR-2, PR-5, VPE gamma, AtrbohD and AtrbohF were highly expressed when the mutant plants were grown under non-sterile conditions. The expression of the AtPDR8 gene was enhanced by infection of virulent and avirulent bacterial pathogens. Our results indicate that AtPDR8 is a key factor controlling the extent of cell death in the defense response and suggest that AtPDR8 transports some substance(s) which is closely related to the response of plants to pathogens.

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  178. Rewiring mitogen-activated protein kinase cascade by positive feedback confers potato blight resistance Reviewed

    C Yamamizo, K Kuchimura, A Kobayashi, S Katou, K Kawakita, JDG Jones, N Doke, H Yoshioka

    PLANT PHYSIOLOGY   Vol. 140 ( 2 ) page: 681 - 692   2006.2

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    Late blight, caused by the notorious pathogen Phytophthora infestans, is a devastating disease of potato (Solanum tuberosum) and tomato (Solanum lycopersicum), and during the 1840s caused the Irish potato famine and over one million fatalities. Currently, grown potato cultivars lack adequate blight tolerance. Earlier cultivars bred for resistance used disease resistance genes that confer immunity only to some strains of the pathogen harboring corresponding avirulence gene. Specific resistance gene-mediated immunity and chemical controls are rapidly overcome in the field when new pathogen races arise through mutation, recombination, or migration from elsewhere. A mitogen-activated protein kinase (MAPK) cascade plays a pivotal role in plant innate immunity. Here we show that the transgenic potato plants that carry a constitutively active form of MAPK kinase driven by a pathogen-inducible promoter of potato showed high resistance to early blight pathogen Alternaria solani as well as P. infestans. The pathogen attack provoked defense-related MAPK activation followed by induction of NADPH oxidase gene expression, which is implicated in reactive oxygen species production, and resulted in hypersensitive response-like phenotype. We propose that enhancing disease resistance through altered regulation of plant defense mechanisms should be more durable and publicly acceptable than engineering overexpression of antimicrobial proteins.

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  179. Disruption of AtPDR8, a plasma membrane ABC transporter of <i>Arabidopsis</i>, exhibits hypersensitive cell death by pathogen infection. Reviewed

    Kobae, Y, Yoshioka, H, Sekino, T, Nakanishi, Y, Martinoia, E, Maeshima, M

    Plant & Cell Physiology   Vol. 47   page: 309-318   2006

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  180. Subcellular localization of Strboh proteins and NADPH-dependent O 2--generating activity in potato tuber tissues Reviewed

    Michie Kobayashi, Kazuhito Kawakita, Masayoshi Maeshima, Noriyuki Doke, Hirofumi Yoshioka

    Journal of Experimental Botany   Vol. 57 ( 6 ) page: 1373 - 1379   2006

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    Rapid generation of reactive oxygen species (ROS) at the cell surface has been implicated in plant defence responses. Genetic evidence indicates that a plant NADPH oxidase (Rboh
    respiratory burst oxidase homologue) is associated with oxidative burst. However, there is not enough physiological evidence of Rboh localization available yet. Isozyme-specific antibodies against potato StrbohA and StrbohB (St
    Solanum tuberosum) were prepared to investigate the localization of these proteins. Immunoblot analyses using potato microsomal proteins revealed that StrbohA was expressed constitutively at a low level, whereas the accumulation of StrbohB protein was induced by the cell wall elicitor of the potato pathogen Phytophthora infestans. It is demonstrated here that StrbohA and StrbohB are distributed in plasma membrane fractions which have been separated by sucrose density-gradient centrifugation using their specific antibodies. Green fluorescent protein-tagged Strboh proteins were also located on the plasma membrane by transient expression assay in onion epidermal cells. Additionally, NADPH-dependent O2--generating activities in plasma membrane fractions were diphenylene iodonium-sensitive and NaN 3-insensitive. These data suggest that StrbohA and StrbohB are predominantly localized on the plasma membrane and regulate ROS production in defence signalling. © The Author [2006]. Published by Oxford University Press [on behalf of the Society for Experimental Biology]. All rights reserved.

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  181. 植物の感染応答因子としてのNO. Invited

    川北一人, 山本(加藤, 文子, 齋藤修平, 加藤新平, 加藤大明, 浅井秀太, 樹神博士, 吉岡博文, 道家紀志

    病原体の寄生戦略と植物の応答   Vol. 42   page: 11-22   2006

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  182. Involvement of PPS3 phosphorylated by elicitor-responsive mitogen-activated protein kinases in the regulation of plant cell death Reviewed

    S Katou, H Yoshioka, K Kawakita, O Rowland, JDG Jones, H Mori, N Doke

    PLANT PHYSIOLOGY   Vol. 139 ( 4 ) page: 1914 - 1926   2005.12

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    Mitogen-activated protein kinase (MAPK) cascades play pivotal roles in plant innate immunity. Overexpression of StMEK1(DD), a constitutively active MAPK kinase that activates salicylic acid-induced protein kinase (SIPK) and wound-induced protein kinase (WIPK), provokes hypersensitive response-like cell death in Nicotiana benthamiana. Here we purified a 51-kD MAPK, which was activated in potato (Solanum tuberosum) tubers treated with hyphal wall elicitor of a plant pathogen, and isolated the cDNA designated StMPK1. The deduced amino acid sequence of the StMPK1 showed strong similarity to stress-responsive MAPKs, such as tobacco (Nicotiana tabacum) SIPK and Arabidopsis (Arabidopsis thaliana) AtMPK6. To investigate the downstream signaling of StMPK1, we identified several proteins phosphorylated by StMPK1 (PPSs) using an in vitro expression cloning method. To dissect the biological function of PPSs in the plant defense, we employed virus-induced gene silencing (VIGS) in N. benthamiana. VIGS of NbPPS3 significantly delayed cell death induced by the transient expression of StMEK1(DD) and treatment with hyphal wall elicitor. Furthermore, the mobility shift of NbPPS3 on SDS-polyacrylamide gel was induced by transient expression of StMEK1(DD). The mobility shift of NbPPS3 induced by StMEK1(DD) was not compromised by VIGS of WIPK or SIPK alone, but drastically reduced by the silencing of both WIPK and SIPK. This work strongly supports the idea that PPS3 is a physiological substrate of StMPK1 and is involved in cell death activated by a MAPK cascade.

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  183. 抵抗性遺伝子を介した防御応答におけるシグナル伝達機構. Invited

    吉岡博文, 山溝千尋, 小林光智衣, 水野貴仁, 川北一人, 道家紀志

    植物・病原体相互反応における特異性決定機構   Vol. 41   page: 13-22   2005.8

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  184. The Arabidopsis gene CAD1 controls programmed cell death in the plant immune system and encodes a protein containing a MACPF domain Reviewed

    C Morita-Yamamuro, T Tsutsui, M Sato, H Yoshioka, M Tamaoki, D Ogawa, H Matsuura, T Yoshihara, A Ikeda, Uyeda, I, J Yamaguchi

    PLANT AND CELL PHYSIOLOGY   Vol. 46 ( 6 ) page: 902 - 912   2005.6

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    To clarify the processes involved in plant immunity, we have isolated and characterized a single recessive Arabidopsis mutant, cad1 (constitutively activated cell death 1), which shows a phenotype that mimics the lesions seen in the hypersensitive response (HR). This mutant shows spontaneously activated expression of pathogenesis-related (PR) genes, and leading to a 32-fold increase in salicylic acid (SA). Inoculation of cad1 mutant plants with Pseudomonas syringae pv tomato DC3000 shows that the cad1 mutation results in the restriction of bacterial growth. Cloning of CAD1 reveals that this gene encodes a protein containing a domain with significant homology to the MACPF (membrane attack complex and perforin) domain of complement components and perforin proteins that are involved in innate immunity in animals. Furthermore, cell death is suppressed in transgenic cad1 plants expressing nahG, which encodes an SA-degrading enzyme. We therefore conclude that the CAD1 protein negatively controls the SA-mediated pathway of programmed cell death in plant immunity.

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  185. Primary lung adenocarcinoma with heterotopic bone formation.

    Usami N, Yoshioka H, Mori S, Imaizumi M, Nagasaka T, Ueda Y

    The Japanese journal of thoracic and cardiovascular surgery : official publication of the Japanese Association for Thoracic Surgery = Nihon Kyobu Geka Gakkai zasshi   Vol. 53 ( 2 ) page: 102 - 5   2005.2

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    DOI: 10.1007/s11748-005-0010-1

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  186. Functional analysis of Avr9/Cf-9 rapidly elicited genes identifies a protein kinase, ACIK1, that is essential for full Cf-9-dependent disease resistance in tomato Reviewed

    O Rowland, AA Ludwig, CJ Merrick, F Baillieul, FE Tracy, WE Durrant, L Fritz-Laylin, Nekrasov, V, K Sjolander, H Yoshioka, JDG Jones

    PLANT CELL   Vol. 17 ( 1 ) page: 295 - 310   2005.1

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    Tomato (Lycopersicon esculentum) Cf genes confer resistance to the fungal pathogen Cladospodium fulvum through recognition of secreted avirulence (Avr) peptides. Plant defense responses, including rapid alterations in gene expression, are immediately activated upon perception of the pathogen. Previously, we identified a collection of Avr9/Cf-9 rapidly (15 to 30 min) elicited (ACRE) genes from tobacco (Nicotiana tabacum). Many of the ACRE genes encode putative signaling components and thus may play pivotal roles in the initial development of the defense response. To assess the requirement of 42 of these genes in the hypersensitive response (HR) induced by Cf-9/Avr9 or by Cf-4/Avr4, we used virus-induced gene silencing (VIGS) in N. benthamiana. Three genes were identified that when silenced compromised the Cf-mediated HR. We further characterized one of these genes, which encodes a Ser/Thr protein kinase called Avr9/Cf-9 induced kinase 1 (ACIK1). ACIK1 mRNA was rapidly upregulated in tobacco and tomato upon elicitation by Avr9 and by wounding. Silencing of ACIK1 in tobacco resulted in a reduced HR that correlated with loss of ACIK1 transcript. Importantly, ACIK1 was found to be required for Cf-9/Avr9- and Cf-4/Avr4-mediated HRs but not for the HR or resistance mediated by other resistance/Avr systems, such as Pto/AvrPto, Rx/Potato virus X, or N/Tobacco mosaic virus. Moreover, VIGS of LeACIK1 in tomato decreased Cf-9-mediated resistance to C. fulvum, showing the importance of ACIK1 in disease resistance.

    DOI: 10.1105/tpc.104.026013

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  187. Role of calcium ion in plant innate immunity

    Yoshioka, H; Ooura, I; Kawakita, K; Doke, N

    PLANT AND CELL PHYSIOLOGY   Vol. 46   page: S23 - S23   2005

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  188. 植物における活性酸素の役割とその誘導.活性酸素,-生産と防御のメカニズム-. Invited

    吉岡博文, 山溝千尋

    蛋白質 核酸 酵素   Vol. 50   page: 310-315   2005

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  189. Involvement of nitric oxide generation in hypersensitive cell death induced by elicitin in tobacco cell suspension culture.

    Yamamoto, A, Katou, S, Yoshioka, H, Doke, N, Kawakita, K

    Journal of Genaral Plant Pathology   Vol. 70   page: 85-92   2004.1

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  190. Cytosolic HSP90 and HSP70 are essential components of INF1-mediated hypersensitive response and non-host resistance to <i>Pseudomonas cichorii</i> in <i>Nicotiana benthamiana</i>

    Kanzaki, H; Saitoh, H; Ito, A; Fujisawa, S; Kamoun, S; Katou, S; Yoshioka, H; Terauchi, R

    MOLECULAR PLANT PATHOLOGY   Vol. 4 ( 5 ) page: 383 - 391   2003.9

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    DOI: 10.1046/J.1364-3703.2003.00186.X

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  191. Disease stress-inducible genes of tobacco: expression profile of elicitor-responsive genes isolated by subtractive hybridization

    Takemoto, D; Yoshioka, H; Doke, N; Kawakita, K

    PHYSIOLOGIA PLANTARUM   Vol. 118 ( 4 ) page: 545 - 553   2003.8

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    DOI: 10.1034/j.1399-3054.2003.00145.x

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  192. Commensurate-incommensurate transition in a one-dimensional dimerized electron system close to quarter-filling

    Yoshioka, H; Tsuchiizu, M; Suzumura, Y

    SYNTHETIC METALS   Vol. 133   page: 49 - 50   2003.3

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    DOI: 10.1016/S0379-6779(02)00252-7

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  193. Elicitation of primary and secondary metabolism during defense in the potato. Reviewed

    Nakane, E, Kawakita, K, Doke, N, Yoshioka, H

    Journal of Genaral Plant Pathology   Vol. 69   page: 378-384   2003.1

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  194. Nitrate reductase, a nitric oxide-producing enzyme: induction by pathogen signals. Reviewed

    Yamamoto, A, Katou, S, Yoshioka, H, Doke, N, Kawakita, K

    Journal of Genaral Plant Pathology   Vol. 69   page: 218-229   2003.1

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  195. Salmonella pericarditis in a patient with primary idiopathic chylopericardium.

    Yoshioka H, Shigemitsu K, Takeuchi M, Mori S, Imaizumi M, Ueda Y

    The Japanese journal of thoracic and cardiovascular surgery : official publication of the Japanese Association for Thoracic Surgery = Nihon Kyobu Geka Gakkai zasshi   Vol. 51 ( 1 ) page: 16 - 7   2003.1

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    DOI: 10.1007/s11748-003-0059-7

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  196. Direct interaction of OsRac with NADPH oxidase regulates ROS production in defense signaling of rice

    Pinontoan, R; Yoshioka, H; Kawasaki, T; Shimamoto, K

    PLANT AND CELL PHYSIOLOGY   Vol. 44   page: S178 - S178   2003

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  197. Functional analysis of potato mitogen-activated protein kinase kinase, StMEK1. Reviewed

    Katou, S, Yamamoto, A, Yoshioka. H, Kawakita, K, Doke, N

    Journal of General Plant Pathology   Vol. 69 ( 3 ) page: 161-168 - 168   2003

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  198. 宿主植物の抵抗性制御と糸状菌病に対する耐病性強化の展望. Invited

    吉岡博文, 口村和男, 山溝千尋, 池田直希, 加藤新平, 川北一人, 小林 晃, 道家紀志

    作物の耐病性強化戦略と植物-病原体相互作用の分子機構研究   Vol. 39   page: 101-110   2003

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  199. 植物免疫としてのオキシダティブバーストを探る,-MAPKカスケードが活性酸素の生成と細胞死を制御する-. Invited

    吉岡博文

    化学と生物   Vol. 41   page: 639-641   2003

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  200. Perception of pathogen signals and molecular mechanisms for the oxidative burst

    Yoshioka, H

    PLANT AND CELL PHYSIOLOGY   Vol. 43   page: S10 - S10   2002

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  201. Induction of plant gp91 <i>phox</i> homolog by fungal cell wall, arachidonic acid, and salicylic acid in potato. Reviewed

    Yoshioka, H, Sugie, K, Park, H.-J, Maeda, H, Tsuda, N, Kawakita, K, Doke, N

    Molecular Plant-Microbe Interactions   Vol. 14   page: 725-736   2001.1

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  202. The oxidative burst in plants: Mechanism and function in induced resistance

    Doke, N; Park, HJ; Katou, S; Komatsubara, H; Makino, T; Ban, S; Sugie, K; Yoshioka, H; Kawakita, K

    DELIVERY AND PERCEPTION OF PATHOGEN SIGNALS IN PLANTS     page: 184 - 193   2001

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  203. Transport properties of disordered carbon nanotubes with long-range Coulomb interaction

    Yoshioka, H

    PHYSICAL REVIEW B   Vol. 61 ( 11 ) page: 7316 - 7319   2000.3

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    DOI: 10.1103/PhysRevB.61.7316

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  204. CDNA cloning of sesquiterpene cyclase and squalene synthase, and expression of the genes in potato tuber infected with Phytophthora infestans Reviewed

    H Yoshioka, N Yamada, N Doke

    PLANT AND CELL PHYSIOLOGY   Vol. 40 ( 9 ) page: 993 - 998   1999.9

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    Sesquiterpene cyclase and squalene synthase are key branch point enzymes in isoprenoid pathway for the synthesis of sesquiterpenoid phytoalexins and sterols/steroid glycoalkaloids, respectively. cDNA clones encoding these enzymes were isolated from potato, A phylogenetic tree showed that the sesquiterpene cyclase is vetispiradiene synthase. Infection of Phytophthora infestans with potato tubers caused transient increases in the transcript level of vetispiradiene synthase in a compatible and an incompatible interactions. On the other hand, wound-induced expression of the squalene synthase was suppressed in favor of the expression of vetispiradiene synthase regardless of inoculated races.

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  205. A 51 kDa protein kinase of potato activated with hyphal wall components from <i>Phytophthora infestans</i>

    Katou, S; Senda, K; Yoshioka, H; Doke, N; Kawakita, K

    PLANT AND CELL PHYSIOLOGY   Vol. 40 ( 8 ) page: 825 - 831   1999.8

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    DOI: 10.1093/oxfordjournals.pcp.a029611

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  206. Resistance of carbon nanotubes connected to reservoirs

    Yoshioka, H; Odintsov, AA

    SYNTHETIC METALS   Vol. 103 ( 1-3 ) page: 2527 - 2528   1999.6

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    DOI: 10.1016/S0379-6779(98)01091-1

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  207. Theoretical study on the charge gap of organic conductor - Bechgaard salts

    Tsuchiizu, M; Yoshioka, H; Suzumura, Y

    JOURNAL OF THE PHYSICAL SOCIETY OF JAPAN   Vol. 68 ( 6 ) page: 1809 - 1812   1999.6

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    DOI: 10.1143/JPSJ.68.1809

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  208. Aspartate aminotransferase in alfalfa nodules: Localization of mRNA during effective and ineffective nodule development and promoter analysis

    Yoshioka, H; Gregerson, RG; Samac, DA; Hoevens, KCM; Trepp, G; Gantt, JS; Vance, CP

    MOLECULAR PLANT-MICROBE INTERACTIONS   Vol. 12 ( 4 ) page: 263 - 274   1999.4

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    DOI: 10.1094/MPMI.1999.12.4.263

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  209. Persistent current and correlation effects in carbon nanotubes

    Odintsov, AA; Smit, W; Yoshioka, H

    EUROPHYSICS LETTERS   Vol. 45 ( 5 ) page: 598 - 604   1999.3

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    DOI: 10.1209/epl/i1999-00209-3

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  210. NADH-glutamate synthase in alfalfa root nodules. Genetic regulation and cellular expression

    Trepp, GB; van de Mortel, M; Yoshioka, H; Miller, SS; Samac, DA; Gantt, JS; Vance, CP

    PLANT PHYSIOLOGY   Vol. 119 ( 3 ) page: 817 - 828   1999.3

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    DOI: 10.1104/pp.119.3.817

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  211. Effect of cytochalasin D on defense responses of potato tuber discs treated with hyphal wall components of <i>Phytophthora infestans</i>

    Takemoto, D; Maeda, H; Yoshioka, H; Doke, N; Kawakita, K

    PLANT SCIENCE   Vol. 141 ( 2 ) page: 219 - 226   1999.2

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    DOI: 10.1016/S0168-9452(98)00238-6

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  212. Aspartate aminotransferase in alfalfa nodules: Localization of mRNA during effective and ineffective nodules development and promoter analysis. Reviewed

    Yoshioka, H, Gregerson, R.G, Samac, D.A, Hoevens, K.C.M, Trepp, G, Gantt, J.S, Vance, C.P

    Molecular Plant-Microbe Interactions   Vol. 12   page: 263-274   1999.1

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  213. NADH-glutamate synthase in alfalfa root nodules: genetic regulation and cellular expression. Reviewed

    Trepp, G, van de Mortel, M, Yoshioka, H, Miller, S.S, Samac, D.A, Gantt, J.S, Vance, C.P

    Plant Physiology   Vol. 119   page: 817-828   1999.1

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  214. Plasma membrane perturbation in association with calcium ion movement followed by fungal elicitor-stimulated oxidative burst and defense gene activation in potato tuber. Reviewed

    Miura, Y, Yoshioka, H, Park, H.-J, Kawakita, K, Doke, N

    Annals of the Phytopathological Society of Japan   Vol. 65 ( 4 ) page: 447-453 - 453   1999.1

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    Chlortetracycline (CTC) is known to fluoresce when interacting with Ca^<2+> in hydrophobic environments such as membranes. CTC fluorescence in potato tubers was rapidly reduced by hyphal wall component-elicitor (HWC-elicitor) from Phytophthora infestans in a dose-dependent manner, suggesting that Ca^<2+> in the plasma membrane was released by the elicitor. Pre-treatments of the tuber slices with verapamil and EGTA, a Ca^<2+> channel blocker and an extracellular Ca^<2+> chelator, respectively, suppressed the reduction of CTC fluorescence induced by the HWC-elicitor. The reduced fluorescence was preceded by an oxidative burst observed in the tuber tissues loaded with luminol after stimulation with HWC-elicitor. Application with HWC-elicitor also immediately reduced fluorescence of the CTC-loaded, Ca^<2+>-bound plasma membrane fraction in vitro. Moreover, RNA get blot analysis showed that verapamil and EGTA dramatically suppressed the accumulation of transcript for phenylalanine ammonia-lyase induced by the elicitor. These results demonstrate that extracellular Ca^<2+> is rapidly released from the plasma membrane by the treatment with HWC-elicitor and plays a key role in signal transduction leading to the oxidative burst and the activation of a defense gene.

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  215. Response functions of two-coupled chains of Tomonaga-Luttinger liquids

    Yoshioka, H; Suzumura, Y

    PHYSICA C   Vol. 309 ( 1-2 ) page: 151 - 160   1998.12

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    DOI: 10.1016/S0921-4534(98)00518-8

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  216. ジャガイモ塊茎における防御遺伝子発現とそのシグナル伝達. Invited

    吉岡博文, 伊藤夢子, 杉江顕一, 三浦由雄, 川北一人, 道家紀志

    植物と病原体の情報応答機構     page: 49-108   1998

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  217. Analyses of phosphoenolpyruvate carboxylase gene structure and expression in alfalfa nodules

    Pathirana, MS; Samac, DA; Roeven, R; Yoshioka, H; Vance, CP; Gantt, JS

    PLANT JOURNAL   Vol. 12 ( 2 ) page: 293 - 304   1997.8

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    DOI: 10.1046/j.1365-313X.1997.12020293.x

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  218. Nitrogen assimilation in alfalfa: Isolation and characterization of an asparagine synthetase gene showing enhanced expression in root nodules and dark-adapted leaves

    Shi, LF; Twary, SN; Yoshioka, H; Gregerson, RG; Miller, SS; Samac, DA; Gantt, JS; Unkefer, PJ; Vance, CP

    PLANT CELL   Vol. 9 ( 8 ) page: 1339 - 1356   1997.8

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    DOI: 10.1105/tpc.9.8.1339

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  219. Properties of fluctuations in two coupled chains of Luttinger liquids

    Yoshioka, H; Suzumura, Y

    JOURNAL OF LOW TEMPERATURE PHYSICS   Vol. 106 ( 1-2 ) page: 49 - 72   1997.1

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    DOI: 10.1007/BF02403915

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  220. Topological dislocations and mixed state of charge density waves

    Hayashi, M; Yoshioka, H

    PHYSICAL REVIEW LETTERS   Vol. 77 ( 16 ) page: 3403 - 3406   1996.10

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1103/PhysRevLett.77.3403

    Web of Science

    PubMed

  221. A cytosolic phospholipase A<sub>2</sub> from potato tissues appears to be patatin. Reviewed

    Senda, K, Yoshioka, H, Doke, N, Kawakita, K

    Plant and Cell Physiology   Vol. 37   page: 347-353   1996.1

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    Language:English   Publishing type:Research paper (scientific journal)  

  222. ROLES OF INTERCHAIN HOPPING IN 2 COUPLED CHAINS OF TOMONAGA MODEL

    YOSHIOKA, H; SUZUMURA, Y

    JOURNAL OF THE PHYSICAL SOCIETY OF JAPAN   Vol. 64 ( 10 ) page: 3811 - 3825   1995.10

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    DOI: 10.1143/JPSJ.64.3811

    Web of Science

  223. Suppression of the activation of chitinase and β-1,3-glucanase in pea epicotyls by eogenous suppressor from <i>Pisam sativum</i>. Reviewed

    Nasu, K, Yoshioka, H, Ichinose, Y, Yamada, T, Oku, H, Shiraishi, T

    Annals of the Phytopathological Society of Japan   Vol. 61   page: 13-17   1995.1

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    Language:English   Publishing type:Research paper (scientific journal)  

  224. Suppression of phenylalanine ammonia-lyase mRNA accumulation by suppressors from <i>Phytophthora infestans</i>. Reviewed

    Yoshioka, H, Hayakawa, Y, Doke, N

    Annals of the Phytopathological Society of Japan   Vol. 61 ( 1 ) page: 7-12   1995

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    Language:English   Publishing type:Research paper (scientific journal)  

  225. THE X-RAY TELESCOPE ON BOARD ASCA

    SERLEMITSOS, PJ; JALOTA, L; SOONG, Y; KUNIEDA, H; TAWARA, Y; TSUSAKA, Y; SUZUKI, H; SAKIMA, Y; YAMAZAKI, T; YOSHIOKA, H; FURUZAWA, A; YAMASHITA, K; AWAKI, H; ITOH, M; OGASAKA, Y; HONDA, H; UCHIBORI, Y

    PUBLICATIONS OF THE ASTRONOMICAL SOCIETY OF JAPAN   Vol. 47 ( 1 ) page: 105 - 114   1995

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    Language:English   Publishing type:Research paper (scientific journal)  

    Web of Science

  226. SUPPRESSORS - DETERMINANTS OF SPECIFICITY PRODUCED BY PLANT-PATHOGENS Invited Reviewed

    T SHIRAISHI, T YAMADA, K SAITOH, T KATO, K TOYODA, H YOSHIOKA, HM KIM, Y ICHINOSE, M TAHARA, H OKU

    PLANT AND CELL PHYSIOLOGY   Vol. 35 ( 8 ) page: 1107 - 1119   1994.12

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:JAPANESE SOC PLANT PHYSIOLOGISTS  

    Plant pathogens secrete suppressors that delay or prevent the host defense responses, with resultant conditioning of host cells such that they become susceptible even to avirulent or nonpathogenic microorganisms. Suppressors have been characterized as glycoproteins, glycopeptides, peptides and anionic and nonanionic glucans. A suppressor itself is non-toxic to plant cells and, thus, it can be distinguished from host-specific toxins produced by certain pathogens. Suppressors disturb fundamental functions of host plasma membranes. For example, the suppressor from a pea pathogen, Mycosphaerella pinodes, inhibits both the ATPase activity and polyphosphoinositide metabolism in pea plasma membranes, causing the temporary suppression of the signal-transduction pathway that leads to the expression of defense genes, which encode key enzymes in the biosynthetic pathway to phytoalexin. In this review, evidence for the role of suppressors in the determination of plant host-parasite specificity is summarized.

    Web of Science

  227. Race-cultivar specificity in plant fungal disease and its molecular mechanisms. Invited

    Plant Cell Technology   Vol. 5 ( 1 ) page: 19-27   1993

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    Language:Japanese   Publishing type:Research paper (scientific journal)  

  228. AN ENDOGENOUS SUPPRESSOR OF THE DEFENSE RESPONSE IN PISUM-SATIVUM Reviewed

    K NASU, T SHIRAISHI, H YOSHIOKA, N HORI, Y ICHINOSE, T YAMADA, H OKU

    PLANT AND CELL PHYSIOLOGY   Vol. 33 ( 5 ) page: 617 - 626   1992.7

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    Healthy pea plants contain a substance, tentatively called "endogenous suppressor", which specifically suppresses the accumulation of pisatin in pea plants that is induced by treatment with CuCl2 or an elicitor from Mycosphaerella pinodes. This suppressor elicits the accumulation of phytoalexins in other legumes, such as kidney bean, soybean and cowpea. The endogenous suppressor functions to delay the accumulation of pisatin, the activation of phenylalanine ammonialyase (PAL) and the accumulation of mRNAs for PAL and chalcone synthase induced by the elicitor from M. pinodes. The substance specifically induces susceptibility to nonpathogens, such as Mycosphaerella ligulicola and M. melonis, in pea out of four species of legume tested, but the effect is not cultivar-specific. Thus, the endogenous suppressor in healthy pea plants suppresses a series of self-defense reactions and induces susceptibility in pea plants in a species-specific manner, being similar to the exogenous fungal suppressor from the pea pathogen, M. pinodes.

    Web of Science

  229. PREPARATION OF THE INFECTION COURT BY ERYSIPHE-GRAMINIS F-SP HORDEI - CUTINASE IS A COMPONENT OF THE CONIDIAL EXUDATE Reviewed

    SF PASCHOLATI, H YOSHIOKA, H KUNOH, RL NICHOLSON

    PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY   Vol. 41 ( 1 ) page: 53 - 59   1992.7

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:ACADEMIC PRESS LTD  

    Web of Science

  230. REGULATION OF POLYPHOSPHOINOSITIDE METABOLISM IN PEA PLASMA-MEMBRANES BY ELICITOR AND SUPPRESSOR FROM A PEA PATHOGEN, MYCOSPHAERELLA-PINODES Reviewed

    K TOYODA, T SHIRAISHI, H YOSHIOKA, T YAMADA, Y ICHINOSE, H OKU

    PLANT AND CELL PHYSIOLOGY   Vol. 33 ( 4 ) page: 445 - 452   1992.6

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:OXFORD UNIV PRESS  

    Effects of the elicitor and the suppressor from a pea pathogen, Mycosphaerella pinodes, on polyphosphoinositide metabolism in pea plasma membranes were examined in vitro. Lipid phosphorylation in the isolated pea plasma membrane was drastically stimulated by the elicitor, but markedly inhibited by the suppressor. A similar inhibitory effect was observed by the treatment with orthovanadate or K-252a that blocked pisatin production induced by the elicitor. Neomycin, an aminoglycoside antibiotic that interacts with the polyphosphoinositide metabolism, also affected the lipid phosphorylation in vitro and blocked the elicitor-induced accumulation of pisatin in vivo. These results suggest that rapid changes of polyphosphoinositide metabolism in pea plasma membranes is one of indispensable processes during the elicitation of defense responses.

    Web of Science

  231. ORTHOVANADATE SUPPRESSES ACCUMULATION OF PHENYLALANINE AMMONIA-LYASE MESSENGER-RNA AND CHALCONE SYNTHASE MESSENGER-RNA IN PEA EPICOTYLS INDUCED BY ELICITOR FROM MYCOSPHAERELLA-PINODES Reviewed

    H YOSHIOKA, T SHIRAISHI, S KAWAMATA, K NASU, T YAMADA, Y ICHINOSE, H OKU

    PLANT AND CELL PHYSIOLOGY   Vol. 33 ( 2 ) page: 201 - 204   1992.3

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    Orthovanadate delayed accumulation of mRNAs encoding phenylalanine ammonia-lyase and chalcone synthase in pea epicotyls induced by an elicitor from Mycosphaerella pinodes. However, accumulation of mRNA for a putative P-type ATPase was not affected. The relationship between the ATPase and defense responses is discussed.

    Web of Science

  232. Suppression of the activation of chitinase and β-1,3-glucanase in pea epicotyls by orthonadate and a suppressor from <i>Mycosphaerella pinodes</i>. Reviewed

    Yoshioka, H, Shiraishi, T, Yamada, T, Nasu, K, Ichinose, Y, Oku, H

    Annals of the Phytopathological Society of Japan   Vol. 58 ( 3 ) page: 405-410   1992

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  233. SUPPRESSOR PRODUCTION AS A KEY FACTOR FOR FUNGAL PATHOGENESIS Reviewed

    T SHIRAISHI, T YAMADA, H OKU, H YOSHIOKA

    MOLECULAR STRATEGIES OF PATHOGENS AND HOST PLANTS     page: 151 - 162   1991

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    Language:English   Publishing type:Research paper (international conference proceedings)   Publisher:SPRINGER-VERLAG  

    Web of Science

  234. エンドウ動的遺伝子の発現機構. Invited

    山田哲治, 白石友紀, 一瀬勇規, 奥 八郎, 川又伸治, 田中良和, Spirasertsak, P, 安 成才, 吉岡博文, 橋本忠明

    生物相互間の認識機構   Vol. 27   page: 56-65   1991

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    Language:Japanese   Publishing type:Research paper (scientific journal)  

  235. SUPPRESSION OF PISATIN PRODUCTION AND ATPASE ACTIVITY IN PEA PLASMA-MEMBRANES BY ORTHOVANADATE, VERAPAMIL AND A SUPPRESSOR FROM MYCOSPHAERELLA-PINODES Reviewed

    H YOSHIOKA, T SHIRAISHI, T YAMADA, Y ICHINOSE, H OKU

    PLANT AND CELL PHYSIOLOGY   Vol. 31 ( 8 ) page: 1139 - 1146   1990.12

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:JAPANESE SOC PLANT PHYSIOLOGISTS  

    A pea pathogen, Mycosphaerella pinodes, secretes both an elicitor and a suppressor for the accumulation of pisatin, a major phytoalexin of pea, into the spore germination fluid. The effects of elicitor and the suppressor on the ATPase activity in pea plasma membranes was examined. The ATPase was sensitive to orthovanadate and dicyclohexylcarbodiimide but insensitive to nitrate and azide; it was unaffected by the elicitor but was markedly inhibited by the suppressor (50-mu-g.ml-1, bovine serum albumin equivalents) or verapamil (100-mu-M). The accumulation of pisatin induced by the elicitor was delayed for 3 to 6 h in the presence of orthovanadate or verapamil to an extent similar to that in the presence of the suppressor. The relationship between the inhibition of plasma membrane ATPase activity and the suppression of the active defense reaction that involves the production of pisatin in the pea plant is discussed.

    Web of Science

  236. PREPARATION OF THE INFECTION COURT BY ERYSIPHE-GRAMINIS - DEGRADATION OF THE HOST CUTICLE Reviewed

    H KUNOH, RL NICHOLSON, H YOSIOKA, N YAMAOKA, KOBAYASHI, I

    PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY   Vol. 36 ( 5 ) page: 397 - 407   1990.5

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:ACADEMIC PRESS LTD  

    Web of Science

  237. PREPARATION OF THE INFECTION COURT BY ERYSIPHE-GRAMINIS .1. CONTACT-MEDIATED CHANGES IN MORPHOLOGY OF THE CONIDIUM SURFACE Reviewed

    H KUNOH, N YAMAOKA, H YOSHIOKA, RL NICHOLSON

    EXPERIMENTAL MYCOLOGY   Vol. 12 ( 4 ) page: 325 - 335   1988.12

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS  

    Web of Science

  238. PREPARATION OF THE INFECTION COURT BY ERYSIPHE-GRAMINIS .2. RELEASE OF ESTERASE ENZYME FROM CONIDIA IN RESPONSE TO A CONTACT STIMULUS Reviewed

    RL NICHOLSON, H YOSHIOKA, N YAMAOKA, H KUNOH

    EXPERIMENTAL MYCOLOGY   Vol. 12 ( 4 ) page: 336 - 349   1988.12

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS  

    Web of Science

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Books 40

  1. 活性酸素シグナルによる植物免疫応答の分子機構

    吉岡博文・日野雄太・岡本渓太・稲田太一・小川尊也・安達広明・吉岡美樹( Role: Joint author)

    日本植物病理学会  2023 

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    Total pages:11   Language:Japanese Book type:Scholarly book

  2. 抵抗性誘導剤を内包したナノ粒子の開発

    山口賢人・北川隆啓・飛永恭兵・斉藤太香雄・吉岡博文・鳴坂義弘( Role: Joint author)

    日本農薬学会誌  2022 

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    Total pages:4   Language:Japanese Book type:Scholarly book

  3. ナノ粒子による植物免疫システムの誘導と病害防除戦略

    吉岡博文・吉岡美樹・鳴坂義弘・鳴坂真理・大高剛史・上田真澄( Role: Joint author)

    アグリバイオ,北隆館  2021.3 

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    Total pages:5   Responsible for pages:75-79   Language:Japanese Book type:Textbook, survey, introduction

  4. 植物病理学 第2版

    吉岡博文( Role: Joint author)

    文永堂出版  2020.3 

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    Language:Japanese Book type:Textbook, survey, introduction

  5. 植物を元気にして病気を防ぐ―植物活力剤によるイチゴの病害抑制技術―

    鳴坂義弘・鳴坂真理・吉岡博文・吉岡美樹・谷口伸治・石川美友紀・紀岡雄三・野口勝憲( Role: Joint author)

    植物防疫,日本植物防疫協会  2019 

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    Total pages:5   Responsible for pages:684-688   Language:Japanese Book type:Textbook, survey, introduction

  6. In vivo phosphorylation of WRKY transcription factor by MAPK. "Plant MAP Kinases: Methods and Protocols", In Methods in Molecular Biology

    Ishihama, N., Adachi, H., Yoshioka, M. and Yoshioka, H.( Role: Joint author)

    Springer/Humana Press  2014.6 

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    Language:English

  7. Tracer, -植物免疫を応用した耐病性作物の作出をめざして-

    吉岡博文( Role: Sole author)

    名古屋大学アイソトープ総合センター  2012.10 

     More details

    Language:Japanese

  8. Nitric oxide and reactive oxygen species in plant defense responses. In Genome-Enabled Analysis of Plant-Pathogen Interactions.

    Yoshioka, H., Asai, S., Yoshioka, M., Kobayashi, M. and Doke, N.( Role: Joint author)

    APS Press  2011 

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    Language:English

  9. Riboflavin synthesis participates in plant immune responses in Nicotiana benthamiana. In Biology of Plant-Microbe Interactions Vol. 7. Chapter 28, Proceedings of the 14th International Congress on Molecular Plant-Microbe Interactions.

    Asai, S. Mase, K. and Yoshioka, H.( Role: Joint author)

    IS-MPMI Press  2010 

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    Language:English

  10. 植物病理学

    吉岡博文( Role: Joint author)

    文永堂出版  2010.2 

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    Language:Japanese Book type:Textbook, survey, introduction

  11. Molecular mechanisms of the radical burst in plant immunity. In Molecular Plant-Microbe Interactions.

    Yoshioka, H., Asai, S., Miyagawa, N., Ichikawa, T., Yoshioka, M. and Kobayashi, M.( Role: Joint author)

    CAB International  2009 

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    Language:English

  12. 植物ゲノム科学辞典

    ( Role: Joint author)

    朝倉書店  2009 

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    Language:Japanese

  13. エリシターの受容とオキシダティブバーストの分子機構とその機能.微生物の病原性と植物の防御応答.

    道家紀志・川北一人・吉岡博文( Role: Joint author)

    北海道大学出版会  2007 

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    Language:Japanese

  14. The superoxide-generating NADPH oxidase in plants: its molecular basis and role in induction of local and systemic induced resistance against pathogens. In Genomic and Genetic Analysis of Plant Parasitism and Defense

    Doke, N., Yoshioka, H., Sugie, K., Numata, N., Nakajima, K., Park, H.-J., Sunazaki, K., Yamashita, M., Kawakita K. and Katou, S.( Role: Joint author)

    APS press  2005 

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    Language:English

  15. 感染植物のオキシダティブバースト:その機構・機能と耐病性化への応用.

    道家紀志・吉岡博文・川北一人( Role: Joint author)

    「植物病の探求」出版会  2004 

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    Language:Japanese

  16. 新版 分子レベルからみた植物の耐病性,-ポストゲノム時代の植物免疫研究-,植物の生体防御機構.

    吉岡博文・川北一人・道家紀志( Role: Joint author)

    秀潤社  2004 

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    Language:Japanese

  17. Mechanism of local and systemic oxidative bursts induced by infection or elicitor in potato. In Biology of Plant-Microbe Interactions Vol. 3

    Doke, N., Yoshioka, H., Kawakita, K., Sugie, K., Sunazaki, K. and Park, H.-J.( Role: Joint author)

    IS-MPMI Press  2002 

     More details

    Language:English

  18. The oxidative burst in plants: mechanism and function in induced resistance. In Delivery and Perception of Pathogen Signals in Plants.

    Doke, N., Park, H.-J., Katou, S., Komatsubara, H., Makino, T., Ban, S., Sugie, K., Yoshioka, H. and Kawakita, K.( Role: Joint author)

    APS press  2001 

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    Language:English

  19. Molecular cloning, expression, and characterization of the cDNA and gene for sesquiterpene cyclase in potato. In Plant Diseases and Their Control: The Selected Papers of The First Asian Conference on Plant Patholgy.

    Yoshioka, H., Kuchimura, K. and Doke, N.( Role: Joint author)

    China Agricultural Scientech Press  2001 

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    Language:English

  20. The oxidative burst system in plants : a strategic signal transduction system for triggering active defense and for parasites to overcome. In Molecular Genetics of Host-Specific Toxins in Plant Diseases.

    Doke, N., Sanchez, L.M., Yoshioka, H., Kawakita, K., Miura, Y. and Park, H.-J.( Role: Joint author)

    Kluwer Academic Publishers  1998 

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    Language:English

  21. Plant immunization in potato culture: its basis and application. In Proceedings of the Fourth APA Triennial Conference Vol. 2

    Doke, N., Hikota, T., Kawamura, H., Miura, Y., Kawakita, K., Yoshioka, H. and Umemura, Y.( Role: Joint author)

    APA press  1994 

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    Language:English

  22. Signal transduction and hypersensitive reaction in Phytophthora-solanaceae plant pathosystem: superoxide generation reaction. In Host-Specific Toxin Biosynthesis, Receptor and Molecular Biology.

    Doke, N., Miura, Y., Sanchez, L.M., Yoshioka, H. and Kawakita, K.( Role: Joint author)

    Tottori University press  1994 

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    Language:English

  23. Suppressor production as a key factor for fungal pathogenesis. In Molecular Strategies of Pathogens and Host Plants.

    Shiraishi, T., Yamada, T., Oku, H. and Yoshioka, H.( Role: Joint author)

    Springer-Verlag  1991 

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    Language:English

  24. 植物病理学 第2版

    吉岡博文( Role: Joint author)

    文永堂出版  2020.3 

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    Responsible for pages:306-307.   Language:Japanese Book type:Textbook, survey, introduction

  25. In vivo phosphorylation of WRKY transcription factor by MAPK. "Plant MAP Kinases: Methods and Protocols", <i>In</i> Methods in Molecular Biology

    Ishihama, N, Adachi, H, Yoshioka, M, Yoshioka, H( Role: Joint author)

    Springer/Humana Press  2014.6 

     More details

    Responsible for pages:171-181.   Language:English

  26. Tracer, -植物免疫を応用した耐病性作物の作出をめざして-

    吉岡博文( Role: Sole author)

    名古屋大学アイソトープ総合センター  2012.10 

     More details

    Responsible for pages:3-7.   Language:Japanese

  27. Nitric oxide and reactive oxygen species in plant defense responses. <i>In</i> Genome-Enabled Analysis of Plant-Pathogen Interactions.

    Yoshioka, H, Asai, S, Yoshioka, M, Kobayashi, M, Doke, N( Role: Joint author)

    APS Press  2011 

     More details

    Responsible for pages:47-55.   Language:English

  28. 植物病理学

    吉岡博文( Role: Joint author)

    文永堂出版  2010.2 

     More details

    Responsible for pages:222-224.   Language:Japanese Book type:Textbook, survey, introduction

  29. Riboflavin synthesis participates in plant immune responses in <i>Nicotiana benthamiana</i>. <i>In</i> Biology of Plant-Microbe Interactions Vol. 7. Chapter 28, Proceedings of the 14th International Congress on Molecular Plant-Microbe Interactions.

    Asai, S. Mase, Yoshioka, H( Role: Joint author)

    IS-MPMI Press  2010 

     More details

    Responsible for pages:Chapter 28   Language:English

  30. Molecular mechanisms of the radical burst in plant immunity. <i>In</i> Molecular Plant-Microbe Interactions.

    Yoshioka, H, Asai, S, Miyagawa, N, Ichikawa, T, Yoshioka, M, Kobayashi, M( Role: Joint author)

    CAB International  2009 

     More details

    Responsible for pages:59-74   Language:English

  31. 植物ゲノム科学辞典

    ( Role: Joint author)

    朝倉書店  2009 

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    Responsible for pages:25,43,70,375   Language:Japanese

  32. エリシターの受容とオキシダティブバーストの分子機構とその機能.微生物の病原性と植物の防御応答.

    道家紀志, 川北一人, 吉岡博文( Role: Joint author)

    北海道大学出版会  2007 

     More details

    Responsible for pages:32-43   Language:Japanese

  33. The superoxide-generating NADPH oxidase in plants: its molecular basis and role in induction of local and systemic induced resistance against pathogens. <i>In</i> Genomic and Genetic Analysis of Plant Parasitism and Defense

    Doke, N, Yoshioka, H, Sugie, K, Numata, N, Nakajima, K, Park, H.-J, Sunazaki, K, Yamashita, M, Kawakita K, Katou, S( Role: Joint author)

    APS press  2005 

     More details

    Responsible for pages:195-206   Language:English

  34. 感染植物のオキシダティブバースト:その機構・機能と耐病性化への応用.

    道家紀志, 吉岡博文, 川北一人( Role: Joint author)

    「植物病の探求」出版会  2004 

     More details

    Responsible for pages:28-34   Language:Japanese

  35. 新版 分子レベルからみた植物の耐病性,-ポストゲノム時代の植物免疫研究-,植物の生体防御機構.

    吉岡博文, 川北一人, 道家紀志( Role: Joint author)

    秀潤社  2004 

     More details

    Responsible for pages:103-110   Language:Japanese

  36. Mechanism of local and systemic oxidative bursts induced by infection or elicitor in potato. <i>In</i> Biology of Plant-Microbe Interactions Vol. 3

    Doke, N, Yoshioka, H, Kawakita, K, Sugie, K, Sunazaki, K, Park, H.-J( Role: Joint author)

    IS-MPMI Press  2002 

     More details

    Responsible for pages:113-117   Language:English

  37. Molecular cloning, expression, and characterization of the cDNA and gene for sesquiterpene cyclase in potato. <i>In</i> Plant Diseases and Their Control: The Selected Papers of The First Asian Conference on Plant Patholgy.

    Yoshioka, H, Kuchimura, K, Doke, N( Role: Joint author)

    China Agricultural Scientech Press  2001 

     More details

    Responsible for pages:36-40   Language:English

  38. The oxidative burst system in plants : a strategic signal transduction system for triggering active defense and for parasites to overcome. <i>In</i> Molecular Genetics of Host-Specific Toxins in Plant Diseases.

    Doke, N, Sanchez, L.M, Yoshioka, H, Kawakita, K, Miura, Y, Park, H.-J( Role: Joint author)

    Kluwer Academic Publishers  1998 

     More details

    Responsible for pages:331-341   Language:English

  39. Plant immunization in potato culture: its basis and application. <i>In</i> Proceedings of the Fourth APA Triennial Conference Vol. 2

    Doke, N, Hikota, T, Kawamura, H, Miura, Y, Kawakita, K, Yoshioka, H, Umemura, Y( Role: Joint author)

    APA press  1994 

     More details

    Responsible for pages:29-36   Language:English

  40. Signal transduction and hypersensitive reaction in <i>Phytophthora</i>-solanaceae plant pathosystem: superoxide generation reaction. <i>In</i> Host-Specific Toxin Biosynthesis, Receptor and Molecular Biology.

    Doke, N, Miura, Y, Sanchez, L.M, Yoshioka, H, Kawakita, K( Role: Joint author)

    Tottori University press  1994 

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    Responsible for pages:153-167   Language:English

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MISC 143

  1. ナノ粒子による植物免疫システムの誘導と病害防除戦略 Invited

    吉岡博文・吉岡美樹・鳴坂義弘・鳴坂真理・大高剛史・上田真澄

    アグリバイオ   Vol. 3 ( 5 ) page: 75 - 79   2021.3

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    Authorship:Lead author   Language:Japanese  

  2. 植物を元気にして病気を防ぐ ―植物活力剤によるイチゴの病害抑制技術― Invited Reviewed

    鳴坂義弘・鳴坂真理・吉岡博文・吉岡美樹・谷口伸治・石川美友紀・紀岡雄三・野口勝憲

    植物防疫   Vol. 73 ( 11 ) page: 684-688   2019.11

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  3. 植物免疫における活性酸素生成誘導のしくみ—植物はいかにして病原菌から身を護るのか? Invited

    安達広明・吉岡博文

    化学と生物   Vol. 55 ( 9 ) page: 20 - 26   2017

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  4. Spatiotemporal dynamics of SA and JA signalings in response to various pathogens in Nicotiana benthamiana.

    吉岡美樹, 荒川花子, 西内沙希, 鳴坂義弘, 鳴坂真理, 北川隆啓, 斉藤太香雄, 安達広明, 吉岡博文

    日本植物病理学会報   Vol. 88 ( 1 )   2022

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  5. Functional analysis of Ralstonia solanacearum effector clone99 which suppresses HR-like cell death by MEK2<sup>DD</sup>.

    伊藤悠揮, 大路美咲, 枝木美樹, 北村理人, 田口義人, 吉岡博文, 秋光和也, 市村和也

    日本植物病理学会報   Vol. 87 ( 1 )   2021

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  6. Research for development of biostimulant to protect agricultural crops.

    鳴坂真理, 高野義孝, 谷口伸治, 藤澤英司, 野口勝憲, 吉岡美樹, 吉岡博文, 鳴坂義弘

    日本植物病理学会報   Vol. 87 ( 1 )   2021

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  7. Ralstonia solanacearum effector with ankyrin repeat suppressed MEK2<sup>DD</sup>-induced hypersensitive response-like cell death in Nicotiana benthamiana.

    土屋慧宜, 枝木美樹, 北村理人, 田口義人, 吉岡博文, 秋光和也, 市村和也

    日本植物病理学会報   Vol. 87 ( 1 )   2021

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  8. Proteome analyses of ROS sensor proteins mediating ROS immune signaling.

    岡本渓太, 小川尊也, 稲田太一, 安達広明, 吉岡美樹, 森仁志, 吉岡博文

    日本植物病理学会報   Vol. 86 ( 1 )   2020

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  9. Chloroplastic ROS signaling is involved in chloroplasts clustering around the nucleolus in plant immune responses.

    小川尊也, 岡本渓太, 稲田太一, 安達広明, 吉岡美樹, 杉浦大輔, 吉岡博文

    日本植物病理学会報   Vol. 86 ( 1 )   2020

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  10. ROS sensor proteins mediating plant immune signaling positively regulate defense responses

    岡本渓太, 小川尊也, 稲田太一, 安達広明, 吉岡美樹, 森仁志, 吉岡博文

    日本植物病理学会大会プログラム・講演要旨予稿集   Vol. 2020   2020

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  11. Pharmacological studies on spatiotemporal dynamics of plant immune MAPK activity induced by feeding of 28-spotted ladybug.

    岩田啓一朗, 高橋来人, 千賀紀尚, 安達広明, 石濱伸明, 吉岡美樹, 近藤竜彦, 吉岡博文

    日本植物病理学会報   Vol. 86 ( 1 )   2020

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  12. Dynamics of plant immune MAPK activity: spatiotemporal imaging using Arabidopsis expressing a MAPK biosensor.

    吉岡美樹, 安達広明, 石濱伸明, 鳴坂真理, 鳴坂義弘, 高野義孝, 吉岡博文

    日本植物病理学会報   Vol. 86 ( 1 )   2020

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  13. Biosensor for salicylate and jasmonate signalings reflecting their antagonistic responses

    田中達己, 安達広明, 吉岡美樹, 荒川花子, 別役重之, 別役重之, 多田安臣, 鳴坂真理, 鳴坂義弘, 吉岡博文

    日本植物病理学会大会プログラム・講演要旨予稿集   Vol. 2020   2020

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  14. 低分子化合物および未利用資源による植物保護剤の開発研究

    鳴坂義弘, 山次康幸, 吉岡美樹, 吉岡博文, 裏地美杉, 畑中唯史, 鳴坂真理

    日本植物病理学会報   Vol. 84 ( 1 ) page: 18(J‐STAGE)   2018

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  15. 免疫応答におけるROSセンサータンパク質の関与

    稲田太一, 安達広明, 吉岡美樹, 吉岡博文

    日本植物病理学会報   Vol. 84 ( 1 )   2018

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  16. 免疫応答においてMAPK活性を可視化するバイオセンサーの開発

    安達広明, 石濱伸明, 吉美樹, 鳴坂真理, 鳴坂義弘, 吉岡博文

    日本植物病理学会大会プログラム・講演要旨予稿集   Vol. 2017   page: 47   2017.4

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  17. 低分子化合物および未利用資源によるバイオスティミュラントの開発研究

    鳴坂義弘, 山次康幸, 吉岡美樹, 吉岡博文, 裏地美杉, 畑中唯史, 鳴坂真理

    日本生化学会大会(Web)   Vol. 90th   page: ROMBUNNO.3P‐1256 (WEB ONLY)   2017

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  18. 植物免疫における活性酸素生成誘導のしくみ 植物はいかにして病原菌から身を護るのか?

    安達広明, 吉岡博文

    化学と生物   Vol. 55 ( 9 )   2017

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  19. 植物の過敏感反応様細胞死を抑制する青枯病菌アンキリンリピートエフェクターの解析

    枝木美樹, 北村理人, 田口義人, 吉岡博文, 秋光和也, 市村和也

    日本植物病理学会報   Vol. 83 ( 1 )   2017

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  20. 環境にやさしい病害防除剤プラントアクティベーターの開発研究

    鳴坂義弘, 山次康幸, 大原利章, 吉岡博文, 鳴坂真理

    日本植物細胞分子生物学会大会・シンポジウム講演要旨集   Vol. 34th   page: 144   2016.8

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  21. MAPKの基質となるWRKY型転写因子の葉緑体ROSバーストへの関与

    安達広明, 波多江健太, 佐藤昌直, 吉岡博文

    日本植物病理学会大会プログラム・講演要旨予稿集   Vol. 2016   2016

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  22. 葉緑体による活性酸素生成を介した植物免疫機構

    吉岡博文, 安達広明

    日本生体防御学会学術総会講演抄録集   Vol. 27th   2016

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  23. イネOsRLCK185によるNADPHオキシダーゼのリン酸化活性化機構

    吉岡美樹, 山口公志, 藤原正幸, 吉村智美, 川崎努, 吉岡博文

    日本植物病理学会大会プログラム・講演要旨予稿集   Vol. 2015   page: 96   2015.3

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  24. Kinase-mediated orchestration of NADPH oxidase in plant immunity. Invited Reviewed

    Adachi, H, Yoshioka, H

    Briefings in Functional Genomics   Vol. 14 ( 4 ) page: 253-259.   2015.3

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  25. PAMPsに応答したWRKY8転写因子のリン酸化動向

    波多江健太, 安達広明, 吉岡美樹, 吉岡博文

    日本植物病理学会大会プログラム・講演要旨予稿集   Vol. 2015   2015

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  26. RNA-seqによるMAPK-WRKY経路下流の植物免疫関連遺伝子の探索

    安達広明, 佐藤昌直, 吉岡博文

    日本植物病理学会大会プログラム・講演要旨予稿集   Vol. 2015   2015

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  27. RNA-seqによるMAPK-WRKY経路下流の免疫応答誘導機構の解析

    安達広明, 佐藤昌直, 吉岡博文

    日本植物生理学会年会要旨集   Vol. 56th   2015

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  28. CDPKおよびRLCKがNADPHオキシダーゼを介したROSバーストを制御する

    白石佑太郎, 吉岡美樹, 安達広明, 山口公志, 川崎努, 吉岡博文

    日本植物病理学会大会プログラム・講演要旨予稿集   Vol. 2014   page: 87   2014.5

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  29. MAPK‐WRKY経路は抵抗性遺伝子に依存したNbRBOHBの転写活性化に関与する

    安達広明, 石濱伸明, 中野孝明, 宮川典子, 吉岡美樹, 八丈野孝, 白須賢, 吉岡博文

    日本植物病理学会大会プログラム・講演要旨予稿集   Vol. 2014   page: 87   2014.5

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  30. エフェクター認識後のNbRBOHBプロモーター活性化に複数のWRKY型転写因子が関与する

    安達広明, 石濱伸明, 中野孝明, 宮川典子, 吉岡美樹, 八丈野孝, 白須賢, 吉岡博文

    日本植物生理学会年会要旨集   Vol. 55th   page: 171   2014.3

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  31. イネのOsRLCK185はNADPHオキシダーゼであるOsRBOHIを介して活性酸素生成を誘導する

    吉岡美樹, 白石佑太郎, 山口公志, 川崎努, 吉岡博文

    日本植物病理学会報   Vol. 80 ( 1 ) page: 28 - 29   2014.2

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  32. 複数のWRKY型転写因子は抵抗性遺伝子に依存した活性酸素生産に関与する

    安達広明, 石濱伸明, 中野孝明, 宮川典子, 吉岡美樹, 八丈野孝, 白須賢, 吉岡博文

    日本植物病理学会報   Vol. 80 ( 1 ) page: 28   2014.2

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  33. ベンサミアナタバコのアミノアシラーゼはジャガイモ疫病菌に対する基礎的抵抗性を負に制御する

    中野真人, 西原昌宏, 吉岡博文, 木場章範

    日本植物学会大会研究発表記録   Vol. 78th   2014

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  34. 植物の免疫応答に関与するMAPキナーゼの恒常活性型変異体の作出

    波多江健太, 安達広明, 吉岡美樹, 吉岡博文

    日本植物病理学会大会プログラム・講演要旨予稿集   Vol. 2014   2014

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  35. リン酸化修飾によるNADPHオキシダーゼの活性化には複数のCDPKが関与する

    白石佑太郎, 吉岡美樹, 安達広明, 吉岡博文

    日本植物病理学会報   Vol. 80 ( 1 )   2014

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  36. WRKY型転写因子は病害応答性MAPKの下流で誘導されるROS生産の重要な制御因子である

    安達広明, 石濱伸明, 吉岡美樹, 加藤有里, 八丈野孝, 白須賢, 吉岡博文

    日本植物病理学会大会プログラム・講演要旨予稿集   Vol. 2013   page: 49   2013.3

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  37. WRKY型転写因子が病害応答性MAPKに依存したROS生産に関与する

    安達広明, 石濱伸明, 吉岡美樹, 加藤有里, 八丈野孝, 白須賢, 吉岡博文

    日本植物生理学会年会要旨集   Vol. 54th   page: 287   2013.3

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  38. 病害応答性MAPKの下流で複数のWRKY型転写因子が防御応答の制御に関与する

    安達広明, 石濱伸明, 吉岡美樹, 加藤有里, 八丈野孝, 白須賢, 吉岡博文

    日本植物病理学会報   Vol. 79 ( 1 ) page: 44   2013.2

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  39. StCDPK5のVドメインは細胞内局在およびNADPHオキシダーゼの認識・活性化に寄与する

    浅井秀太, 市川達士, 野村裕也, 小林光智衣, 上吉原裕介, 森仁志, 門田康弘, ZIPFEL Cyril, JONES Jonathan, 吉岡博文

    日本植物病理学会大会プログラム・講演要旨予稿集   Vol. 2013   2013

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  40. リン酸化による植物NADPHオキシダーゼの制御機構

    吉岡博文, 安達広明, 白石佑太郎, 中野孝明, 宮川典子, 浅井秀太, 川崎努, 吉岡美樹

    日本生体防御学会学術総会講演抄録集   Vol. 24th   2013

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  41. ベンサミアナタバコのNADPHオキシダーゼであるNbRBOHBのプロモーター調節にはWRKY型転写因子が関与する

    中野孝明, 安達広明, 宮川典子, 吉岡美樹, 石濱伸明, 吉岡博文

    日本植物病理学会大会プログラム・講演要旨予稿集   Vol. 2013   2013

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  42. WRKY8転写因子を導入した組換えジャガイモは疫病菌および夏疫病菌に抵抗性を示す

    吉岡美樹, 石濱伸明, 金原佳子, 安達広明, 高野義孝, 吉岡博文

    日本植物病理学会報   Vol. 79 ( 1 )   2013

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  43. 新規WRKY型転写因子のMAPKに依存した細胞死誘導への関与

    安達広明, 石濱伸明, 加藤有里, 八丈野孝, 白須賢, 吉岡博文

    日本植物病理学会大会プログラム・講演要旨予稿集   Vol. 2012   page: 84   2012.3

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  44. 病原菌エフェクターの標的であるOsRLCKを介したキチンシグナル伝達機構の解明

    山口公志, 石川和也, 山田健太, 石濱信明, 濱田聡, 津下誠治, 吉岡博文, 島本功, 川崎努

    日本植物生理学会年会要旨集   Vol. 53rd   page: 176   2012.3

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  45. 病害応答性MAPKに依存した細胞死を制御する新規WRKY型転写因子の単離

    安達広明, 石濱伸明, 加藤有里, 八丈野孝, 白須賢, 吉岡博文

    日本植物生理学会年会要旨集   Vol. 53rd   page: 150   2012.3

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  46. 白葉枯病菌エフェクターの新奇標的因子OsRLCKの解析

    山口公志, 石川和也, 石濱信明, 古谷綾子, 落合弘和, 津下誠治, 島本功, 吉岡博文, 川崎努

    日本植物病理学会報   Vol. 78 ( 1 ) page: 46   2012.2

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  47. Regulatory mechanisms of NO and ROS generation and role in plant immunity

    Hirofumi Yoshioka, Keisuke Mase, Miki Yoshioka, Michie Kobayashi, Shuta Asai

    NITRIC OXIDE-BIOLOGY AND CHEMISTRY   Vol. 22   page: S10 - S10   2010.6

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    DOI: 10.1016/j.niox.2010.05.023

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  48. (124) Transgenic Potato Plants Expressing Constitutively Active StCDPK5 Show Late Blight Resistance, but Enhanced Early Blight Susceptibility(Abstracts of the Papers Presented at the Annual Meeting of the Society)

    Yoshioka M., Kobayashi M., Asai S., Yoshioka H.

    Annals of the Phytopathological Society of Japan   Vol. 74 ( 3 ) page: 199 - 200   2008.8

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  49. (314) Involvement of Asparaginerich Protein in Plant Responses to Infection of Bacterial Wilt Pathogen Ralstonia solanacearum(Abstracts of the Papers Presented at the Annual Meeting of the Society)

    Komori D., Ohnishi K., Hikichi Y., Yoshioka H., Kiba A.

    Annals of the Phytopathological Society of Japan   Vol. 74 ( 3 ) page: 250 - 251   2008.8

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  50. (130) The Activation of SIPK and WIPK in Nicotiana benthamiana Is Involved in the Failure of Infection by the cossd1 Mutant of Colletotrichum orbiculare(Abstracts of the Papers Presented at the Annual Meeting of the Society)

    Tanaka S., Ishihama N., Yoshioka H., Tsuji G., Tsuge S., Kubo Y.

    Annals of the Phytopathological Society of Japan   Vol. 74 ( 3 ) page: 201 - 201   2008.8

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  51. (126) Riboflavin Synthesis Participates in Plant Immunity(Abstracts of the Papers Presented at the Annual Meeting of the Society)

    Asai S., Mase K., Yoshioka H.

    Annals of the Phytopathological Society of Japan   Vol. 74 ( 3 ) page: 200 - 200   2008.8

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  52. (125) Functional Regulation of PPS8/StWRKY4 by Phosphorylation(Abstracts of the Papers Presented at the Annual Meeting of the Society)

    Ishihama N., Yoshioka H.

    Annals of the Phytopathological Society of Japan   Vol. 74 ( 3 ) page: 200 - 200   2008.8

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  53. (15)Involvement of NO production-associating Factor NbNOA1 in Defense Responses in Nicotiana benthamiana(Abstracts Presented at the Meeting of the Kansai Division,Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Japan, 2007)

    Kato H., Asai S., Yamamoto-Katou A., Yoshioka H., Doke N., Takemoto D., Kawakita K.

    Annals of the Phytopathological Society of Japan   Vol. 74 ( 1 ) page: 60 - 60   2008.2

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  54. (74)Induction of a Gene Similar to S-locus Glycoprotein and Its Implication in Defense Response of Nicotiana Plants to Ralstonia solanacearum(Abstracts Presented at the Meeting of the Kansai Division,Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Japan, 2007)

    Maimbo M., Ohnishi K., Hikichi Y., Yoshioka H., Kiba A.

    Annals of the Phytopathological Society of Japan   Vol. 74 ( 1 ) page: 75 - 76   2008.2

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  55. (16)NITRIC OXIDE ASSOCIATED1-Mediated NO and NADPH Oxidase-Dependent Oxidative Bursts Play Distinct Roles in Disease Resistance against Phytophthora infestans and Colletotrichum lagenarium(Abstracts Presented at the Meeting of the Kansai Division,Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Japan, 2007)

    Asai S., Yoshioka H.

    Annals of the Phytopathological Society of Japan   Vol. 74 ( 1 ) page: 60 - 60   2008.2

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  56. (124) Peroxinitrate Generation in INF1-Ehcitor Treated Leaves of Nicotiana benthamiana(Abstracts of the Papers Presented at the Annual Meeting of the Society)

    Kodama H., Saito S., Kato H., Yoshioka H., Doke N., Kawakita K.

    Annals of the Phytopathological Society of Japan   Vol. 73 ( 3 ) page: 207 - 208   2007.8

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  57. (56) Superoxide Anion and Nitric Oxide Producing Activities of Ceramidephosphorylethanolamine-Relative Compounds(Abstracts of the Papers Presented at the Annual Meeting of the Society)

    Murakami T., Itou Y., Nozawa T., Ojika H., Yoshioka H., Doke N., Kawakita K.

    Annals of the Phytopathological Society of Japan   Vol. 73 ( 3 ) page: 189 - 189   2007.8

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  58. (355) Silencing of Small Heat Shock Protein Results in Suppression of Defense-related Responses to Bacterial Wilt Pathogen Ralstonia solanacearum(Abstracts of the Papers Presented at the Annual Meeting of the Society)

    Maimbo M., Ohnishi K., Hikichi Y., Yoshioka H., Kiba A.

    Annals of the Phytopathological Society of Japan   Vol. 73 ( 3 ) page: 270 - 270   2007.8

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  59. (135) SIPK and WIPK of Nicotiana benthamiana are Involved in the Failure of Infection by classd1 Mutant of Colletotrichum lagenarium(Abstracts of the Papers Presented at the Annual Meeting of the Society)

    Tanaka S., Ishihama N., Yoshioka H., Tsuji G., Tsuge S., Kubo Y.

    Annals of the Phytopathological Society of Japan   Vol. 73 ( 3 ) page: 210 - 211   2007.8

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  60. (130) Upstream and Downstream Signaling Pathways of Ethylene Are Required for AAL-Toxin-Induced Programmed Cell Death(Abstracts of the Papers Presented at the Annual Meeting of the Society)

    Mizuno T., Mase K., Kawakita K., Doke N., Mori H., Kodama M., Yoshioka H.

    Annals of the Phytopathological Society of Japan   Vol. 73 ( 3 ) page: 209 - 209   2007.8

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  61. (129) Transgenic Potato Plants Expressing Constitutively Active StCDPK5 Show Late Blight Resistance(Abstracts of the Papers Presented at the Annual Meeting of the Society)

    Kobayashi M., Yoshioka M., Kawakita K., Doke N., Yoshioka H.

    Annals of the Phytopathological Society of Japan   Vol. 73 ( 3 ) page: 209 - 209   2007.8

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  62. (128) Fat Modification Region of CDPK Is Involved in StrbohB-Dependent ROS Generation(Abstracts of the Papers Presented at the Annual Meeting of the Society)

    Ichikawa T., Kobayashi M., Kobae Y., Kawakita K., Yoshioka H.

    Annals of the Phytopathological Society of Japan   Vol. 73 ( 3 ) page: 208 - 209   2007.8

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  63. (127) Silencing of NbPPSS Enhances Susceptibility to Phytophthora infestans in Nicotiana benthamiana(Abstracts of the Papers Presented at the Annual Meeting of the Society)

    Ishihama N., Kawakita K., Doke N., Yoshioka H.

    Annals of the Phytopathological Society of Japan   Vol. 73 ( 3 ) page: 208 - 208   2007.8

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  64. (126) Functional Analysis of the Gene Promoter for NADPH Oxidase in Potato(Abstracts of the Papers Presented at the Annual Meeting of the Society)

    Miyagawa N., Yamamizo C., Ishihama N., Kawakita K., Yoshioka H.

    Annals of the Phytopathological Society of Japan   Vol. 73 ( 3 ) page: 208 - 208   2007.8

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  65. (125) MAPK Cascades Regulate NOA1 (NOS1)-Mediated Nitric Oxide and NADPH Oxidase-Mediated Reactive Oxygen Species Generation(Abstracts of the Papers Presented at the Annual Meeting of the Society)

    Asai S., Ohta K., Kato H., Kawakita K., Yoshioka H.

    Annals of the Phytopathological Society of Japan   Vol. 73 ( 3 ) page: 208 - 208   2007.8

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  66. MAPK Cascades Regulate Reactive Oxygen Species and Nitric Oxide Generation(Abstracts Presented at the Meeting of the Kansai Division,Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Japan, 2006)

    Asai S., Ohta K., Kato H., Kawakita K., Yoshioka H.

    Annals of the Phytopathological Society of Japan   Vol. 73 ( 1 ) page: 53 - 53   2007.2

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  67. Involvement of NbNOS1 in NO Production in Nicotiana benthamiana(Abstracts Presented at the Meeting of the Kansai Division,Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Japan, 2006)

    Kato H., Asai S., Kato F., Yoshioka H., Kawakita K.

    Annals of the Phytopathological Society of Japan   Vol. 73 ( 1 ) page: 53 - 53   2007.2

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  68. Screening of Target Genes for PPS8(Abstracts Presented at the Meeting of the Kansai Division,Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Japan, 2006)

    Ishihama N., Oka M., Kawakita K., Doke N., Yoshioka H.

    Annals of the Phytopathological Society of Japan   Vol. 73 ( 1 ) page: 53 - 53   2007.2

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  69. (111) Functional Analysis of Gene for Phytoalexin Synthesis by RNAi in Potato(Abstract of the Paper Presented at the 2006 Annual Meeting in Sapporo)

    Adachi A., Yamamizo C., Yoshioka M., Kawakita K., Doke N., Yoshioka H.

    Annals of the Phytopathological Society of Japan   Vol. 72 ( 4 ) page: 231 - 231   2006.11

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  70. (113) Constitutive Active Mutant of CDPK Induces NbrbohB-dependent ROS Generation(Abstract of the Paper Presented at the 2006 Annual Meeting in Sapporo)

    Kobayashi M., Ohura I., Kawakita K., Doke N., Yoshioka H.

    Annals of the Phytopathological Society of Japan   Vol. 72 ( 4 ) page: 231 - 232   2006.11

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  71. (112) Peroxynitrite Generation in Potato Tuber Infected with Incompatible Race of Phytopthora infestans(Abstract of the Paper Presented at the 2006 Annual Meeting in Sapporo)

    Saito S., Yoshioka H., Doke N., Kawakita K.

    Annals of the Phytopathological Society of Japan   Vol. 72 ( 4 ) page: 231 - 231   2006.11

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  72. (123) Knockdown of MIGST Compromised MEK^<DD> -mediated Cell Death(Abstract of the Paper Presented at the 2006 Annual Meeting in Sapporo)

    Ishihama N., Oka M., Kawakita K., Doke N., Yoshioka H.

    Annals of the Phytopathological Society of Japan   Vol. 72 ( 4 ) page: 234 - 234   2006.11

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  73. (99) Forward Genetic Screening of Genes Involved in Signaling for AAL-toxin by Gene Silencing(Abstract of the Paper Presented at the 2006 Annual Meeting in Sapporo)

    Mase K., Mizuno T., Kawakita K., Doke N., Kodama M., Yoshioka H.

    Annals of the Phytopathological Society of Japan   Vol. 72 ( 4 ) page: 228 - 228   2006.11

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  74. Peroxynitrite Generation in INF1-elicitor Treated Tobacco BY-2 Cells (Abstracts Presented at the Meeting of the Kansai Division,Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Japan 2005)

    Saito S., Yamamoto-Katou A., Yoshioka H., Doke N., Kawakita K.

    Annals of the Phytopathological Society of Japan   Vol. 72 ( 1 ) page: 67 - 67   2006.2

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  75. Functional Analysis of C2H2 type Zn Finger Protein Gene Promoter Responsive to Systemic Oxidative Burst (Abstracts Presented at the Meeting of the Kansai Division,Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Japan 2005)

    Ishihama N., Yoshioka H., Kawakita K., Doke N.

    Annals of the Phytopathological Society of Japan   Vol. 72 ( 1 ) page: 67 - 67   2006.2

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  76. Expression Screening of StrbohB Kinase (Abstracts Presented at the Meeting of the Kansai Division,Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Japan 2005)

    Kobayashi M., Ohura L., Yoshioka H., Kawakita K., Doke N.

    Annals of the Phytopathological Society of Japan   Vol. 72 ( 1 ) page: 67 - 67   2006.2

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  77. Screenig of Target Genes for PPS3, a Substrate of StMPK1 MAPK (Abstracts Presented at the Meeting of the Kansai Division,Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Japan 2005)

    Oka M., Katou S., Kawakita K., Doke N., Yoshioka H.

    Annals of the Phytopathological Society of Japan   Vol. 72 ( 1 ) page: 66 - 66   2006.2

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  78. Role of MAPK Cascade in Disease Resistance to Phytophthora infestans in Potato (Abstracts Presented at the Meeting of the Kansai Division,Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Japan 2005)

    Yamamizo C., Yoshioka M., Hirano M., Kawakita K., Doke N., Yoshioka H.

    Annals of the Phytopathological Society of Japan   Vol. 72 ( 1 ) page: 66 - 67   2006.2

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  79. ABC transporter, AtPDR8 is implicated in pathogen resistance

    Y Kobae, T Sekino, H Yoshioka, T Nakagawa, E Martinoia, M Maeshima

    PLANT AND CELL PHYSIOLOGY   Vol. 47   page: S47 - S47   2006

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  80. (7) in vivo Phosphorylation of N-terminal Region of StrbohB(Abstracts of the Papers Presented at the 2005 Annual Meeting in Shizuoka)

    Kobayashi M., Ohura I., Yoshioka H., Kawakita K., Doke N.

    Annals of the Phytopathological Society of Japan   Vol. 71 ( 3 ) page: 187 - 187   2005.8

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  81. (9) Analysis of Signalling Mechanism of AAL-toxin by Virusinduced Gene Silencing in Tomato(Abstracts of the Papers Presented at the 2005 Annual Meeting in Shizuoka)

    Mizuno T., Ohta K., Kawakita K., Doke N., Kodama M., Yoshioka H.

    Annals of the Phytopathological Society of Japan   Vol. 71 ( 3 ) page: 187 - 187   2005.8

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  82. (8) Production and Analyses of Disease Resistance Transgenic Potato Plants using Potato MAPK, StMPK1(Abstracts of the Papers Presented at the 2005 Annual Meeting in Shizuoka)

    Yamamizo C., Yoshioka M., Kawakita K., Doke N., Yoshioka H.

    Annals of the Phytopathological Society of Japan   Vol. 71 ( 3 ) page: 187 - 187   2005.8

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  83. (6) Characterization of Potato Protein Phosphatase Inactivating MAP kinase (StMPK1)(Abstracts Presented at the Meeting of the Kansai Division, Matsuyama, October 9-10, 2004, Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Japan, 2003)

    Deguchi T., Katou S., Yoshioka H., Doke N., Kawakita K.

    Annals of the Phytopathological Society of Japan   Vol. 71 ( 1 ) page: 53 - 53   2005.2

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  84. (8) Intracellular Localization of Strboh Proteins in Potato Tuber Tissues(Abstracts Presented at the Meeting of the Kansai Division, Matsuyama, October 9-10, 2004, Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Japan, 2003)

    Kobayashi M., Yoshioka H., Kawakita K., Doke N.

    Annals of the Phytopathological Society of Japan   Vol. 71 ( 1 ) page: 54 - 54   2005.2

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  85. (7) Analyses of Transgenic Potato Plants Showing Resistance to Phytophthora infestans(Abstracts Presented at the Meeting of the Kansai Division, Matsuyama, October 9-10, 2004, Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Japan, 2003)

    Yamamizo C., Kuchimura K., Katou S., Kawakita K., Kobayashi A., Doke N., Yoshioka H.

    Annals of the Phytopathological Society of Japan   Vol. 71 ( 1 ) page: 53 - 54   2005.2

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  86. (36) Purification and Characterization of Elicitors from Phytophthora infestans, Responsible for Active Oxygen Species Production(Abstracts of the Papers Presented at the Annual Meeting of the Society, Fukuoka, March 28-30, 2004)

    Ito Y., Yoshioka H., Ojika M., Doke N., Kawakita K.

    Annals of the Phytopathological Society of Japan   Vol. 70 ( 3 ) page: 199 - 200   2004.8

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  87. (65) Expression Analysis of Phospholipase A_2 Genes of Arabidopsis thaliana during Defense Response(Abstracts of the Papers Presented at the Annual Meeting of the Society, Fukuoka, March 28-30, 2004)

    Katayama H., Yoshioka H., Doke N., Kawakita K.

    Annals of the Phytopathological Society of Japan   Vol. 70 ( 3 ) page: 207 - 208   2004.8

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  88. (39) Involvement of Nitrate Reductase in INF1 Elicitor-induced Nitric Oxide Production in Nicotiana benthamiana(Abstracts of the Papers Presented at the Annual Meeting of the Society, Fukuoka, March 28-30, 2004)

    Yamamoto A., Yoshioka H., Doke N., Kawakita K.

    Annals of the Phytopathological Society of Japan   Vol. 70 ( 3 ) page: 200 - 200   2004.8

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  89. (38) Relationship between G6PDH and Defense Responses in Nicotiana benthamiana(Abstracts of the Papers Presented at the Annual Meeting of the Society, Fukuoka, March 28-30, 2004)

    Tone C., Nakajima K., Kawakita K., Doke N., Yoshioka H.

    Annals of the Phytopathological Society of Japan   Vol. 70 ( 3 ) page: 200 - 200   2004.8

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  90. (37) Production of Transgenic Potato Plants Showing Resistance to Phytophtora infestans(Abstracts of the Papers Presented at the Annual Meeting of the Society, Fukuoka, March 28-30, 2004)

    Yamamizo C., Kuchimura K., Katou S., Kawakita K., Doke N., Kobayashi A., Yoshioka H.

    Annals of the Phytopathological Society of Japan   Vol. 70 ( 3 ) page: 200 - 200   2004.8

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  91. (10) Nitric Oxide Profusion and Its Action in Defense Responses in Tobacco Suspension Cells (Abstracts Presented at the Meeting of the Kansai Division) (Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Japan, 2003)

    Yamamoto A., Katou S., Yoshioka H., Doke N., Kawakita K.

    Annals of the Phytopathological Society of Japan   Vol. 70 ( 1 ) page: 58 - 58   2004.2

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  92. (9) Purification of Elicitor(s) from Phytophythora infestans, Responsible for Active Oxygen Species Production (Abstracts Presented at the Meeting of the Kansai Division) (Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Japan, 2003)

    Ito Y., Yoshioka H., Ojika M., Doke N., Kawakita K.

    Annals of the Phytopathological Society of Japan   Vol. 70 ( 1 ) page: 58 - 58   2004.2

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  93. Functional Analysi of PPS Genes by Virus-Induced Gene Silencing(Abstracts of the Papers Presented at the 2003 Annual Meeting in Tokyo)

    Katou S., Yoshioka H., Kawakita K., Doke N.

    Annals of the Phytopathological Society of Japan   Vol. 69 ( 3 ) page: 248 - 248   2003.8

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  94. Elicitor-responsive 36 kDa Protein Kinase Phosphorylates N-terminal Region of StrbohB Proteins(Abstracts of the Papers Presented at the 2003 Annual Meeting in Tokyo)

    Ohura I., Kawakita K., Doke N., Yoshioka H.

    Annals of the Phytopathological Society of Japan   Vol. 69 ( 3 ) page: 249 - 249   2003.8

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  95. Involbement of MAPK Cascade in Hypersensitive Cell Death in Nicotiana benthamiana(Abstracts of the Papers Presented at the 2003 Annual Meeting in Tokyo)

    Ikeda N., Katou S., Kawakita K., Doke N., Yoshioka H.

    Annals of the Phytopathological Society of Japan   Vol. 69 ( 3 ) page: 248 - 249   2003.8

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  96. Functional Analysis of G6PDH by Virus-induced Gene Silencing in Nicotiana benthamiana(Abstracts of the Papers Presented at the 2003 Annual Meeting in Tokyo)

    Tone C., Nakajima K., Katou S., Kawakita K., Doke N., Yoshioka H.

    Annals of the Phytopathological Society of Japan   Vol. 69 ( 3 ) page: 248 - 248   2003.8

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  97. Regulation of MAP Kinase (StMPK1) Activity by Dephosphorylation in Potato Plants(Abstracts of the Papers Presented at the 2003 Annual Meeting in Tokyo)

    Deguchi T., Katou S., Yoshioka H., Doke N., Kawakita K.

    Annals of the Phytopathological Society of Japan   Vol. 69 ( 3 ) page: 248 - 248   2003.8

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  98. Detection of Nitric Oxide Production in Potato Suspension Cells Treated with Elicitor

    Yamamoto A., Katou S., Yoshioka H., Doke N., Kawakita K.

    Annals of the Phytopathological Society of Japan   Vol. 69 ( 1 ) page: 55 - 55   2003.2

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  99. Association of Reactive Oxygen Species with Induction of SAR in Nicotiana benthamiana

    Yamashita M., Numata N., Yoshioka H., Kawakita K., Doke N.

    Annals of the Phytopathological Society of Japan   Vol. 69 ( 1 ) page: 55 - 56   2003.2

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  100. (36)Isolation of PhoM (Protein Phosphorylated by MAP Kinase) 1〜7 Genes and their Expression Analysis

    Katou S., Yamamoto A., Yoshioka H., Kawakita K., Doke N.

    Annals of the Phytopathological Society of Japan   Vol. 68 ( 2 ) page: 165 - 166   2002.8

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  101. (47)Responses to Disease Stresses in Transgenic Tobacco Plants Overexpressing Elicitor Inducible LRR Receptor-like Protein (EILP) Gene

    Katayama H., Takemoto D., Hayashi M., Nishimura M., Yoshioka H., Doke N., Kawakita K.

    Annals of the Phytopathological Society of Japan   Vol. 68 ( 2 ) page: 168 - 169   2002.8

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  102. (37)Studies on Phosphorylation Sites of Strboh Protein by a 36 kDa-Strboh Kinase in Potato Tubers

    Ohura I., Yoshioka H., Katou S., Kawakita K., Doke N.

    Annals of the Phytopathological Society of Japan   Vol. 68 ( 2 ) page: 166 - 166   2002.8

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  103. Mechanism of Signal Transduction for Induction of Systemic Oxidative Burst by Salicylic Acid in Potato Tuber Tissues(Abstracts Presented at the Meeting of the Kansai Division)

    Yamashita M., Sunazaki K., Yoshioka H., Kawakita K., Doke N.

    Annals of the Phytopathological Society of Japan   Vol. 68 ( 1 ) page: 92 - 92   2002.4

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  104. Isolation of Protein Phosphorylated by Elicitor-Responsive MAP Kinase StMPK1 in vitro(Abstracts Presented at the Meeting of the Kansai Division)

    Katou S., Yoshioka H., Kawakita K., Doke N.

    Annals of the Phytopathological Society of Japan   Vol. 68 ( 1 ) page: 91 - 91   2002.4

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  105. Characterization of the Elicitor-Responsive Nitrate Reductase in Potato Plants(Abstracts Presented at the Meeting of the Kansai Division)

    Yamamoto A., Yoshioka H., Doke N., Kawakita K.

    Annals of the Phytopathological Society of Japan   Vol. 68 ( 1 ) page: 91 - 92   2002.4

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  106. Mechanism of Signal Transduction for Induction of Systemic Oxidative Burst in Potato Tuber Tissues

    Sunazaki K., Yoshioka H., Kawakita K., Doke N.

    Annals of the Phytopathological Society of Japan   Vol. 67 ( 2 ) page: 126 - 126   2001.8

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  107. Protein Kinases for StrobohA and StrbohB in Potato Tubers

    Ohura I., Yoshioka H., Katou S., Kawakita K., Doke N.

    Annals of the Phytopathological Society of Japan   Vol. 67 ( 2 ) page: 127 - 127   2001.8

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  108. Molecular Cloning of Nbrboh cDNA from Nicotiana benthamiana

    Numata N., Yoshioka H., Kawakita K., Doke N.

    Annals of the Phytopathological Society of Japan   Vol. 67 ( 2 ) page: 127 - 127   2001.8

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  109. Isolation of the Elicitor-responsive Nitrate Reductase Genes in Potato Plants

    Yamamoto A., Yoshioka H., Doke N., Kawakita K.

    Annals of the Phytopathological Society of Japan   Vol. 67 ( 2 ) page: 126 - 126   2001.8

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  110. Elicitor-responsive Protein Kinase in Potato Plants is MAP Kinase

    Katou S., Senda K., Yoshioka H., Kawakita K., Doke N.

    Annals of the Phytopathological Society of Japan   Vol. 67 ( 2 ) page: 126 - 126   2001.8

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  111. Isolation and Expression Analysis of Elicitor-responsive Genes in Potato by Subtraction(Abstracts Presented at the Meeting of the Kansai Division)

    Ido K., Takemoto D., Yoshioka H., Doke N., Kawakita K.

    Annals of the Phytopathological Society of Japan   Vol. 66 ( 3 ) page: 287 - 287   2000.12

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  112. Involvement of Nitric Oxide in Defense Responses Induced in Potato Plant(Abstracts Presented at the Meeting of the Kansai Division)

    Yamamoto A., Yoshioka H., Doke N., Kawakita K.

    Annals of the Phytopathological Society of Japan   Vol. 66 ( 3 ) page: 286 - 287   2000.12

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  113. Partial Purification of Elicitor-responsive Protein Kinases in Potato Plants(Abstracts Presented at the Meeting of the Kansai Division)

    Katou S., Senda K., Yoshioka H., Kawakita K., Doke N.

    Annals of the Phytopathological Society of Japan   Vol. 66 ( 3 ) page: 287 - 287   2000.12

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  114. Cloning of Strboh cDNA and Expression of the Gene in Potato Tuber

    Sugie K., Yoshioka H., Kawakita K., Doke N.

    Annals of the Phytopathological Society of Japan   Vol. 66 ( 2 ) page: 110 - 110   2000.8

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  115. Signal Mechanisms for Induction of Systemic Oxidative Burst in Plant Tissues:Involvement of Calcium Ion

    Sunazaki K., Yoshioka H., Kawakita K., Doke N.

    Annals of the Phytopathological Society of Japan   Vol. 66 ( 2 ) page: 110 - 110   2000.8

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  116. Cloning and Characterization of Sesquiterpene Cyclase Genes in Potato

    Kuchimura K., Yoshioka H., Yamada N., Doke N.

    Annals of the Phytopathological Society of Japan   Vol. 66 ( 2 ) page: 109 - 110   2000.8

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  117. Induction of Local and Systemic Oxidative Bursts in Plants by Elicitors and Plant Activators (Abstracts Presented at the Meeting of the Kansai Division)

    Sunazaki K., Ban S., Park H. J., Yoshioka H., Kawakita K., Doke N.

    Annals of the Phytopathological Society of Japan   Vol. 65 ( 6 ) page: 690 - 691   1999.12

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  118. Assay of O_2- Generated on Elicitortreated potato Tuber Slices by Electron Spin Resonance (Abstracts Presented at the Meeting of the Kansai Division)

    Park H. J., Yoshioka H., Kawakita K., Doke N.

    Annals of the Phytopathological Society of Japan   Vol. 65 ( 6 ) page: 691 - 691   1999.12

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  119. Tertiary Analysis of Development of Tissue Cells to an Activated State with Oxidative Burst Function in Plant Tissues after Wounding and Infection

    Makino T., Hong S. -K., Park H. -J., Yoshioka H., Kawakita K., Nomura S., Doke N.

    Annals of the Phytopathological Society of Japan   Vol. 65 ( 3 ) page: 323 - 323   1999.6

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  120. Cloning of Sesquiterpene Cyclase and Squalene Synthetase cDNAs in Potato

    Yamada N., Yoshioka H., Doke N.

    Annals of the Phytopathological Society of Japan   Vol. 65 ( 3 ) page: 327 - 327   1999.6

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  121. The Elicitor-responsive Protein Kinase in Potato Plants and Its Characteristics

    Katou S., Senda K., Yoshioka H., Kawakita K., Doke N.

    Annals of the Phytopathological Society of Japan   Vol. 65 ( 3 ) page: 323 - 323   1999.6

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    Language:Japanese   Publisher:The Phytopathological Society of Japan (PSJ)  

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  122. 誘導抵抗性関連研究の最前線. Invited

    吉岡博文

    日植病報バイオコントロール研究会レポート   Vol. 6   page: 19-28   1999

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  123. (1) Induction of Sub-systemic Acquired Resistance and Expression Pattern of GST Gene in Elicitor-treated Potato Tuber Slices (Abstracts Presented at the Meeting of the Kansai Division, 1998)

    Park H.-J., Yoshioka H., Kawakita K., Doke N.

    Annals of the Phytopathological Society of Japan   Vol. 64 ( 6 ) page: 615 - 615   1998.12

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  124. (10) A Novel Signal Transduction for Systemic Acquired Resistance : Induction of a Systemic Oxidative Burst in Tobacco Plants by Treatment with an Elicitor (Abstracts Presented at the Meeting of the Kansai Division, 1998)

    Helmi A., Park H.-J., Yoshioka H., Kawakita K., Doke N.

    Annals of the Phytopathological Society of Japan   Vol. 64 ( 6 ) page: 617 - 617   1998.12

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  125. (2) Cloning of the gp91^<phox>Homologues in Potato (Abstracts Presented at the Meeting of the Kansai Division, 1998)

    Sugie K., Yoshioka H., Tsuda N., Maeda H., Kawakita K., Doke N.

    Annals of the Phytopathological Society of Japan   Vol. 64 ( 6 ) page: 615 - 615   1998.12

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  126. (114) Aspects of Systemic Induction of Oxidative Burst in Fresh and Aged Sliced Surface of Potato Tuber Slices by Treatment with H_2O_2

    Park H.-J., Komatsubara H., Kawakita K., Yoshioka H., Doke N.

    Annals of the Phytopathological Society of Japan   Vol. 64 ( 4 ) page: 355 - 355   1998.8

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  127. (116) Enzymatic Characterization and Partial Purification of O_2-Generating NADPH Oxidase from Plasma Membrane of Potato Tuber Tissues

    Katou S., Tsuda N., Yoshioka H., Kawakita K., Doke N.

    Annals of the Phytopathological Society of Japan   Vol. 64 ( 4 ) page: 356 - 356   1998.8

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  128. (115) Activation and Development of Cells with Oxidative Burst Function in Sliced Tissue and Protoplasts of Potato Tubers

    Komatsubara H., Maeda H., Park H.-J., Yoshioka H., Kawakita K., Doke N.

    Annals of the Phytopathological Society of Japan   Vol. 64 ( 4 ) page: 355 - 356   1998.8

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  129. (171) Changes in Superoxide Generating Response of Potato Tuber Slices to Hyphal Wall Elicitor after Wounding(Abstracts of the Papers Presented at the Annual Meeting of the Society)

    KOMATSUBARA H., PARK H.-J., YOSHIOKA H., KAWAKITA K., DOKE N.

    Annals of the Phytopathological Society of Japan   Vol. 63 ( 3 ) page: 233 - 234   1997.6

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  130. (174) Adhesion and Initial Response of Potato Protoplasts to Cystospores of Phytophthora infestans(Abstracts of the Papers Presented at the Annual Meeting of the Society)

    MAEDA H., TSUDA N., YOSHIOKA H., KAWAKITA K., DOKE N.

    Annals of the Phytopathological Society of Japan   Vol. 63 ( 3 ) page: 234 - 234   1997.6

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  131. (172) A Possible Signal Transduction through a Chain Reaction of Oxidative Burst in Potato Tuber Tissues(Abstracts of the Papers Presented at the Annual Meeting of the Society)

    PARK H.-J., KOMATSUBARA H., KAWAKITA K., YOSHIOKA H., DOKE N.

    Annals of the Phytopathological Society of Japan   Vol. 63 ( 3 ) page: 234 - 234   1997.6

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  132. 植物病原糸状菌のサプレッサーによる宿主防御遺伝子の発現制御機構に関する研究. Invited

    吉岡博文

    日本植物病理学会報   Vol. 63   page: 141   1997.1

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    Language:Japanese   Publishing type:Article, review, commentary, editorial, etc. (scientific journal)  

  133. 植物の感染応答における情報伝達とホスホリパーゼ.植物感染生理学研究の現状と将来展望. Invited

    川北一人, 仙田香織, 鈴木真紀, 吉岡博文, 道家紀志

    植物感染生理学研究の現状と将来展望   Vol. 33   page: 134-142   1997

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  134. Involvement of Inositol Phospholipid-Specific Phospholipase C in Signal Transduction in Potato Suspension Cells Treated with Hyphal Wall Components

    SUZUKI M., YOSHIOKA H., DOKE N., KAWAKITA K.

    Annals of the Phytopathological Society of Japan   Vol. 61 ( 3 ) page: 244 - 244   1995.6

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    Language:Japanese   Publisher:The Phytopathological Society of Japan (PSJ)  

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  135. In vitro Production of Phytoalexin Synthesis Inducing Factor(s) via Lipid Peroxidation in Microsomal Fraction Prepared from Potato Tuber Tissue

    KAWAMURA H., DOKE N., KAWAKITA K., YOSHIOKA H.

    Annals of the Phytopathological Society of Japan   Vol. 61 ( 3 ) page: 245 - 245   1995.6

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    Language:Japanese   Publisher:The Phytopathological Society of Japan (PSJ)  

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  136. Phospholipase A_2 Involved in Induced Resistance Reactions of Potato Tissues

    SENDA K., YOSHIOKA H., DOKE N., KAWAKITA K.

    Annals of the Phytopathological Society of Japan   Vol. 61 ( 3 ) page: 244 - 245   1995.6

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    Language:Japanese   Publisher:The Phytopathological Society of Japan (PSJ)  

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  137. Regulation of Defense Responses in Pea by Fungal Signals, Elicitor and Suppressor from Mycosphaerella pinodes. : (8) In vitro Production of Second Messenger-like Molecule (s)

    KOYAMA M., MIZUKOSHI R., YOSHIOKA H., TOYODA K., ICHINOSE Y., YAMADA T., SHIRAISHI T.

    Annals of the Phytopathological Society of Japan   Vol. 60 ( 3 ) page: 315 - 315   1994.6

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  138. Purification of Cytosolic Phospholipase A_2 from Potato Tuber and Preparation of Antibody against the Enzyme

    SENDA K., YOSHIOKA H., KAWAKITA K., DOKE N.

    Annals of the Phytopathological Society of Japan   Vol. 59 ( 6 ) page: 751 - 752   1993.12

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  139. A Stimulation of Ca^<++>-Movement by the Fungal Elicitor and Its Suppression by Suppressor-Glucans of Phytophthora capsici in Host Suspension-Cultured Cells

    SANCHEZ L.M., DOKE N., MIURA Y., YOSHIOKA H.

    Annals of the Phytopathological Society of Japan   Vol. 59 ( 3 ) page: 267 - 267   1993.6

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  140. Effects of Hyphal Elicitor of Phytophtora infestans on Phospholipid Metabolisms in an Isolated Microsomal Fraction of Potato Tissues

    MIURA Y., YOSHIOKA H., KAWAKITA K., DOKE N.

    Annals of the Phytopathological Society of Japan   Vol. 59 ( 3 ) page: 267 - 268   1993.6

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  141. In vitro Activation of NADPH-O^-_2 Generating Reaction by Elicitor Using Microsomal Fraction from Potato Tuber Tissues

    MIURA Y., KAWAKITA K., YOSHIOKA H., DOKE N.

    Annals of the Phytopathological Society of Japan   Vol. 58 ( 4 ) page: 564 - 564   1992.10

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  142. Effect of AF-toxin I on Phospholipase A_2 Activity in Microsomal Fraction of Strawberry Suspension-cultured Cells

    LEE S.-S., KAWAKITA K., TSUGE T., YOSHIOKA H., DOKE N.

    Annals of the Phytopathological Society of Japan   Vol. 58 ( 4 ) page: 571 - 571   1992.10

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  143. エンドウ動的遺伝子の発現機構. Invited

    山田哲治, 白石友紀, 一瀬勇規, 奥 八郎, 川又伸治, 田中良和, Spirasertsak, P, 安 成才, 吉岡博文, 橋本忠明

    生物相互間の認識機構   Vol. 27   page: 56-65   1991

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Presentations 82

  1. 活性酸素シグナルによる植物免疫応答の分子機構 Invited

    吉岡博文

    植物感染生理学研究の未来  2023  日本植物病理学会

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    Event date: 2023.9

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Venue:岡山   Country:Japan  

  2. Mining of ROS sensor proteins that positively regulate plant immune responses. Invited International conference

    Yoshioka, H., Hino, Y., Okamoto, K., Inada, T., Kondo, T., Mori, H. and Yoshioka, M.

    12th International Conference on the Biology, Chemistly, and Therapeutic Application of Nitric Oxide  2022.10.29 

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    Event date: 2022.10

    Language:English   Presentation type:Oral presentation (invited, special)  

    Venue:Tohoku University   Country:Japan  

  3. Dynamics of plant immune MAPK activity in response to pathogen and insect attacks. Invited International conference

    Yoshioka, H., Hino, Y., Iwata, K., Adachi, H., Ishihama, N. and Yoshioka, M.

    12th Japan-US in Plant Pathology Seminar.  2022.9.2 

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    Event date: 2022.8 - 2022.9

    Language:English   Presentation type:Oral presentation (invited, special)  

    Venue:ornell University, USA   Country:United States  

  4. Plant immunity mediated by MAPK-WRKY phosphorylation pathway Invited International conference

    Hirofumi Yoshioka

    2018 International Symposium on Agricultural Biotechnology 

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    Event date: 2018.11

    Language:English   Presentation type:Oral presentation (invited, special)  

    Venue:Dong-A University   Country:Korea, Republic of  

  5. Plant immune MAPK-WRKY phosphorylation pathway regulates NADPH oxidase and chloroplast-mediated ROS bursts Invited

    Hirofumi Yoshioka

    Stories of oxygen and active molecular species in photosynthetic organisms 

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    Event date: 2018.3

    Language:English   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  6. 植物免疫に関与するMAPキナーゼによる活性酸素生成機構 Invited

    吉岡博文

    第4回新学術領域研究「酸素生物学」公開シンポジウム 

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    Event date: 2017.2

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Venue:九州大学   Country:Japan  

  7. 葉緑体による活性酸素生成を介した植物免疫機構

    吉岡 博文,安達 広明

    「活性酸素が支える生体防御力」第27回 日本生体防御学会学術総会 

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    Event date: 2016.7

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Venue:九州大学   Country:Japan  

  8. Protein kinases hierarchically regulate a plant immune NADPH oxidase. International conference

    Yoshioka, H.

    The 9th International Conference on the Biology, Chemistry, and Therapeutic Applications of Nitric Oxide. 

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    Event date: 2016.5

    Language:English   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  9. 植物免疫に関わるNADPH oxidaseの活性化機構

    吉岡博文

    第38回日本分子生物学会年会第88回日本生化学会大会合同大会 

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    Event date: 2015.12

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Venue:神戸ポートアイランド   Country:Japan  

  10. Protein kinase-mediated regulation of NADPH oxidase in plant immunity. International conference

    Hirofumi Yoshioka, Hiroaki Adachi, Takaaki Nakano, Noriko Miyagawa, Shuta Asai, Nobuaki Ishihama and Miki Yoshioka

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    Event date: 2015.10

    Language:English   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  11. ナス科植物の免疫応答における活性酸素の生成機構

    吉岡博文

    第11回日本ナス科コンソシアム年会 

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    Event date: 2014.10

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Venue: 名古屋大学 坂田・平田ホール   Country:Japan  

  12. Regulatory mechanisms and roles of radical bursts in plant immunity

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    Event date: 2014.5

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  13. Molecular mechanisms of ROS burst conferred by protein phosphorylation

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    Event date: 2013.8

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  14. リン酸化による植物NADPHオキシダーゼの制御機構

    吉岡博文・安達広明・白石佑太郎・中野孝明・宮川典子・浅井秀太・川崎 努・吉岡美樹

    第24回日本生体防御学会学術総会 

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    Event date: 2013.7

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Venue:くまもと森都心プラザ   Country:Japan  

  15. Protein phosphorylation confers ROS burst via NADPH oxidase. International conference

    Yoshioka, H., Asai, S., Nomura, H., Kobayashi, H., Nakano, H., Adachi, H., Ishihama, N. and Yoshioka, M.

    Keystone Symposia "Plant Immunity: Pathways and Translation" 

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    Event date: 2013.4

    Language:English   Presentation type:Oral presentation (invited, special)  

    Country:United States  

  16. 植物免疫における活性酸素生産の分子機構

    吉岡博文

    第3回生体分子研究会 

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    Event date: 2013.3

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Venue:名古屋工業大学   Country:Japan  

  17. Plant immunity.

    Yoshioka, H.

    Symposium on Interdisciplinary Researches in Okayama 

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    Event date: 2012.11

    Language:English   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  18. Molecular mechanisms for generation of NO and ROS in plant immunity. International conference

    Yoshioka, H.

    XV International Congress on Molecular Plant-Microbe Interactions 

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    Event date: 2012.7 - 2012.8

    Language:English   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  19. Phosphorylation of WRKY transcription factor by MAPK and its role in plant immunity. International conference

    Yoshioka, H., Yamada, R., Yoshioka, M. and Ishihama, N.

    SOL & ICuGI 2011, 8th Solanaceae and 2nd Cucurbitaceae Joint Conference 

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    Event date: 2011.11 - 2011.12

    Language:English   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  20. 植物免疫におけるMAPキナーゼカスケードの役割

    吉岡博文

    シンポジウム「動植物における病原体認識と防御」第84回日本生化学会大会 

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    Event date: 2011.9

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Venue:国立京都国際会館,京都   Country:Japan  

  21. 植物免疫応答におけるMAPキナーゼの役割

    吉岡博文

    第3回植物ストレス科学研究シンポジウム並びに第27回資源植物科学シンポジウム 

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    Event date: 2011.3

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Venue:倉敷市芸文館ホール   Country:Japan  

  22. 植物免疫応答における活性酸素・窒素の生成制御機構

    吉岡博文

    「視覚と化学物質による植物の相互作用:発信と受容のメカニズム」第158回生存圏シンポジウム 

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    Event date: 2010.11

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  23. Regulatory mechanisms of NO and ROS generation and role in plant immunity. International conference

    6th International Conference on the Biology, Chemistly, and Therapeutic Application of Nitric Oxide. 

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    Event date: 2010.6

    Language:English   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  24. Regulation of NADPH oxidase-mediated ROS generation and role in plant immunity.

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    Event date: 2010.3

    Language:English   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  25. Nitric oxide and reactive oxygen species in plant defense responses. International conference

    10th Japan-US Seminar: Genome-Enabled Integration of Research in Plant Pathogen Systems. 

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    Event date: 2010.1

    Language:English   Presentation type:Oral presentation (invited, special)  

  26. Molecular mechanisms of generation for nitric oxide and reactive oxygen species, and role of the radical burst in plant immunity. International conference

    The 21st Annual Meeting of the Korean Society for Molecular and Cellular Biology “RNA Biology and Disease" 

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    Event date: 2009.10

    Language:English   Presentation type:Oral presentation (invited, special)  

  27. 活性酸素・活性窒素の生成機構と細胞死における役割

    吉岡博文

    第13回植物バイテクシンポジウム「植物の細胞死-その動態と要因」 

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    Event date: 2009.9

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  28. 植物病害応答におけるCDPKの役割

    吉岡 博文・浅井 秀太・市川 達士・吉岡 美樹・小林 光智衣

    日本植物学会シンポジウム Ca2+シグナルから見た植物細胞の新しい姿 

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    Event date: 2008.9

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  29. Molecular Mechanisms of the Radical Burst in Plant Immunity. International conference

    Molecular Mechanisms of the Radical Burst in Plant Immunity. "Plant Neurobiology 2008", 4th International Symposium on Plant Neurobiology, Fukuoka, Japan 

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    Event date: 2008.6

    Language:English   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  30. Pathogen inducible promoter in plants. International conference

    Technology Licensing and Partnering Forum Life Science Innovations from Chubu-Japan. The Fullerton Hotel, Singapore 

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    Event date: 2007.12

    Language:English   Presentation type:Oral presentation (invited, special)  

  31. MAPKs Signaling Regulates NITRIC OXIDE ASSOCIATED1- Mediated NO and NADPH Oxidase-Dependent Oxidative Bursts in Nicotiana benthamiana

    Hirofumi Yoshioka and Shuta Asai.

    元気な植物作りのためのヒントを探る-植物・微生物相互作用と植物免疫の分子機構- 

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    Event date: 2007.9

    Language:English   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  32. Molecular mechanisms of radical burst in plant immunity

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    Event date: 2007.8

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  33. 病気に負けない作物づくりをめざす -植物がもつ免疫力を利用して- International conference

    吉岡博文

    国際バイオフォーラム 

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    Event date: 2007.6

    Language:Japanese   Presentation type:Poster presentation  

    Country:Japan  

  34. Role of CDPK in plant immunity and its application in the generation of pathogen-resistant potato

    Plant Immunity: From MAMPs/PAMPs Recognition to Gene-for-Gene Resistance 

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    Event date: 2007.3

    Language:English   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  35. 病気に負けない作物づくりをめざす -植物がもつ免疫力を利用して-

    吉岡博文

    生物資源学特別セミナー 

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    Event date: 2007.1

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  36. 植物免疫学で作物を病害から守ることができるのか?

    吉岡博文

    日本分子生物学会2006フォーラム 分子生物学の未来 

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    Event date: 2006.12

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  37. 植物免疫学で作物を病害からまもることができるのか?

    吉岡博文

    先端研究者招へいセミナー 

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    Event date: 2006.11

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  38. 植物免疫に関わるMAPキナーゼの応用と基礎

    吉岡博文

    第3回重点共同利用研究ワークショップ「植物の自己防御と細胞死」 

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    Event date: 2006.3

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  39. Functional mechanism of MAP kinase cascade in plant immunity and its application in the generation of pathogen-resistant potato.

    “Frontiers in Plant Immunity Research"NAIST-Bio COE Symposium, Nara, Japan 

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    Event date: 2006

    Language:English   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  40. MAPK cascade modulates plant innate immunity through enhanced gene expression of rboh gene. International conference

    2nd Asian Conference on Plant Pathology, Singapore 

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    Event date: 2005

    Language:English   Presentation type:Oral presentation (invited, special)  

  41. 抵抗性遺伝子を介した防御応答におけるシグナル伝達機構.

    吉岡博文・山溝千尋・小林光智衣・水野貴仁・川北一人・道家紀志

    植物・病原体相互反応における特異性決定機構.日本植物病理学会,植物感染生理談話会,サンポート高松,高松 

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    Event date: 2005

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  42. Role of calcium ion in plant innate immunity.

    Yoshioka, H., Ohura, I., Kawakita, K. and Doke, N.

    2005年度日本植物生理学会シンポジウム15, 新潟コンベンションセンター朱鷺メッセ, 新潟 

     More details

    Event date: 2005

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  43. Molecular mechanisms of oxidative burst in plant immunity. International conference

    15th International Plant Protection Congress, Beijing, China 

     More details

    Event date: 2004

    Language:English   Presentation type:Oral presentation (invited, special)  

  44. Molecular mechanisms of oxidative burst, a possible role in plant immunity.

    NIAR-COE/BRAIN/CREST Joint International Symposium "Plant Imunity" Signaling to acquired resistance. Tsukuba, Japan 

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    Event date: 2004

    Language:English   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  45. 植物における活性酸素の役割.

    吉岡博文・山溝千尋・口村和男・加藤新平・川北一人・小林 晃・道家紀志

    第15回日本生体防御学会学術総会, 長崎大学医学部ポンペ会館, 長崎市 

     More details

    Event date: 2004

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  46. Plant gp91 phox participate in H2O2 accumulation and resistance of N. benthamiana to P. infestans. International conference

    Yoshioka, H., Numata, N., Nakajima, K., Katou, S., Kawakita, K., Rowland, O., Jones, J.D.G. and Doke, N.

    8th International Congress of Plant Pathology, Christchurch, New Zealand 

     More details

    Event date: 2003

    Language:English   Presentation type:Poster presentation  

  47. 植物の感染防御応答におけるオキシダティブバーストの機構と機能.

    吉岡博文・道家紀志

    活性酸素の機能解明, 木原生物学研究所, 横浜 

     More details

    Event date: 2003

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  48. 宿主植物の抵抗性制御と糸状菌病に対する耐病性強化の展望.

    吉岡博文・口村和男・山溝千尋・池田直希・加藤新平・川北一人・小林 晃・道家紀志

    植物感染生理談話会, 犬山会館, 犬山市 

     More details

    Event date: 2003

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  49. シグナル伝達系酵素遺伝子を利用した耐病性植物の作出.

    吉岡博文

    平成15年度東海植物病研究会, 愛知県農業共済会館, 名古屋市 

     More details

    Event date: 2003

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  50. オキシダティブバ-スト制御による耐病性植物作出に関する研究.

    第2回日本農学進歩賞受賞者講演会, 東京大学農学部弥生講堂, 東京 

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    Event date: 2003

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  51. Nicotiana benthamiana gp91 phox homologs NbrbohA and NbrbohB participate in H2O2 accumulation and resistance to Phytophthora infestans. International conference

    Yoshioka, H., Numata, N., Ikeda, N., Nakajima, K., Katou, S., Kawakita, K., Rowland, O., Jones, J.D.G. and Doke, N.

    Plant Biology 2003, Honolulu, United States 

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    Event date: 2003

    Language:English   Presentation type:Poster presentation  

  52. MAPK cascade regulates gene for plant gp91 phox homologs in Nicotiana benthamiana. International conference

    Yoshioka, H., Numata, N., Ikeda N., Yamamizo, C., Katou, S., Kawakita, K., Rowland, O., Jones, J.D.G. and Doke, N.

    6th conference of Plant Stress, Reactive Oxygen and Antioxidants, Freising-Weihenstephan, Germany, 

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    Event date: 2003

    Language:English   Presentation type:Oral presentation (invited, special)  

  53. Nitrate reductase, a nitric oxide producing enzyme, is induced by pathogen signals in potato plants International conference

    Yamamoto, A., Katou, S., Yoshioka, H., Doke, N. and Kawakita, K.

    11th Congress International Society for Molecular Plant-Microbe Interactions 

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    Event date: 2003.7

    Language:English   Presentation type:Poster presentation  

    St.-Petersburg Russian Federation

  54. Elicitor-stimulated local oxidative burst (OXB) activates a long-distant signaling system involving a cell-to-cell Ca2+ influx depending on H+-ATPase that triggers systemic OXB and systemic acquired resistance (SAR) International conference

    Plant Biology 2003 

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    Event date: 2003.7

    Language:English   Presentation type:Poster presentation  

    Honolulu U.S.A.

  55. 植物の感染防御応答におけるオキシダティブバーストの機構と機能

    吉岡博文・道家紀志

    活性酸素の機能解明 

     More details

    Event date: 2003.3

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

    "木原生物学研究所, 横浜"

  56. Mechanism of dispatching and transduction of early systemic signal for induction of systemic acquired resistance in plants International conference

    8th International Congress of Plant Pathology 

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    Event date: 2003.2

    Language:Japanese   Presentation type:Poster presentation  

    Convection Centre Christchurch New Zealand

  57. 植物のオキシダティブバースト系制御による耐病性強化戦略.

    吉岡博文・口村和男・加藤新平・川北一人・豊田和弘・白石友紀・道家紀志

    見えてきた耐病性植物作出の戦略, 神戸大学, 神戸市 

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    Event date: 2002

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  58. Perception of pathogen signals and molecular mechanisms for the oxidative burst.

    吉岡博文

    Plant Factors Regulating Infection by Pathogen, 2002年度日本植物生理学会第42回シンポジウム, 岡山大学, 岡山市 

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    Event date: 2002

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  59. ナス科植物の過敏感反応における分子制御.

    吉岡博文

    財団法人岩手生物工学研究センター第8回若手研究者セミナー, 岩手県農業研究センター, 北上市 

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    Event date: 2002

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  60. 病原糸状菌ー植物相互作用における宿主オキシダティブバースト系を巡る攻防

    道家紀志・吉岡博文

    第2回糸状菌分子生物学コンファレンス 

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    Event date: 2002.8

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

    "名古屋大学シンポジオン, 名古屋市"

  61. Isolation of the gene for EILP, an elicitor-inducible LRR receptor like protein, from tobacco International conference

    Kawakita, K., Takemoto, D., Katayama, H., Hayashi, M., Yoshioka, H., Doke, N. and Nishimura, M

    10th Congress International Society for Molecular Plant-Microbe Interactions 

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    Event date: 2001.7

    Language:English   Presentation type:Poster presentation  

    University of Wisconsin Madison U.S.A.

  62. Mechanisms of local and systemic oxidative burst induced by infection and elicitor in potato plants International conference

    10th Congress International Society for Molecular Plant-Microbe Interactions 

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    Event date: 2001.7

    Language:English   Presentation type:Oral presentation (invited, special)  

    University of Wisconsin Madison U.S.A.

  63. Purification and characterization of elicitor-responsive MAP kinase in potato plants International conference

    10th Congress International Society for Molecular Plant-Microbe Interactions 

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    Event date: 2001.7

    Language:English   Presentation type:Poster presentation  

    University of Wisconsin Madison U.S.A.

  64. Induction of plant gp91 phox homolog by fungal cell wall, arachidonic acid, and salicylic acid in potato. International conference

    Yoshioka, H., Sugie, K., Park, H.-J., Kawakita, K. and Doke, N.

    10th Congress International Society for Molecular Plant-Microbe Interactions, Madison, United States 

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    Event date: 2001

    Language:English   Presentation type:Poster presentation  

  65. Physiological and molecular mechanisms of oxidative burst in induced resistance in potato plants.

    NIAR-COE/BRAIN/CREST Joint International Symposium "Self-defense signaling pathways in plants". Tsukuba, Japan 

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    Event date: 2000

    Language:English   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  66. 植物の感染防御応答におけるオキシダティブバースト:その機構と機能

    道家紀志・砂崎浩二・杉江顕一・吉岡博文・川北一人

    公開シンポジウム「植物の感染防御における活性酸素とアポトーシス 

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    Event date: 2000.12

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    "名古屋大学シンポジオンホール, 名古屋市"

  67. Involvement of oxidative burst in systemic signaling for induction of systemic acquired resistance in plants International conference

    The first Asian Conference on Plant Pathology 

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    Event date: 2000.8

    Language:English   Presentation type:Poster presentation  

    Beijing China

  68. Involvement of actin filament association in defense responses in potato cells International conference

    The first Asian Conference on Plant Pathology 

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    Event date: 2000.8

    Language:English   Presentation type:Poster presentation  

    Beijing China

  69. 植物の感染・異物に対する認識・防御応答におけるオキシダティブバーストーその現象,機構および機能についてー

    道家紀志・朴海準・小松原肇・三浦由雄・杉江顕一・吉岡博文・川北一人

    2000年度日本農芸化学会大会シンポジウム 

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    Event date: 2000.3

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

    "東京ビッグサイト会議棟, 東京都"

  70. 植物のオキシダティブバーストと感染防御ネットワークの誘導

    道家紀志・朴海準・吉岡博文・川北一人

    植物生理学会第40回シンポジウム 

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    Event date: 2000.3

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

    "椙山女学園, 名古屋市"

  71. Molecular cloning, expression, and characterization of the cDNA and genes for sesquiterpene cyclase in potato. International conference

    The first Asian Conference on Plant Pathology, Beijing, China 

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    Event date: 2000

    Language:English   Presentation type:Oral presentation (general)  

  72. 誘導抵抗性関連研究の最前線.

    吉岡博文

    バイオコントロール研究会, 新潟大学, 新潟市 

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    Event date: 1999

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  73. ジャガイモ塊茎における防御遺伝子発現とそのシグナル伝達.

    吉岡博文・伊藤夢子・杉江顕一・三浦由雄・川北一人・道家紀志

    日本植物病理学会感染生理談話会.植物と病原体の情報応答機構, 神戸大学, 神戸市 

     More details

    Event date: 1998

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  74. Further studies on the oxidative burst in plant defence responses: cell potential and function of the oxidative burst International conference

    7th International Congress of Plant Pathology 

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    Event date: 1998.8

    Language:English   Presentation type:Poster presentation  

    Edinburgh U.K.

  75. The oxidative burst system in plants: a strategic signal transduction system for triggering active defense and for parasites to overcome International conference

    The 3rd International Symposium on Host-Specific Toxin 

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    Event date: 1997.8

    Language:English   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

    Daisen Japan

  76. 植物病原糸状菌のサプレッサーによる宿主防御遺伝子の発現制御機構に関する研究

    吉岡博文

    日本植物病理学会大会奨励賞受賞講演 

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    Event date: 1997.4

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

    国際会議場,名古屋市

  77. Alfalfa aminotransferase-2: in situ expression of mRNA in root nodules and analysis of the putative promoter region of the gene International conference

    The 8th International Symposium on the Molecular Plant-Microbe Interactions 

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    Event date: 1996.8

    Language:English   Presentation type:Poster presentation  

    Tennessee U.S.A.

  78. 植物の感染応答における情報伝達とホスホリパーゼ

    "川北一人, 仙田香織, 鈴木真紀, 吉岡博文, 道家紀志"

    日本植物病理学会感染生理談話会:植物感染生理学研究の現状と将来展望 

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    Event date: 1996.7

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    "蔵王ハイツ, 宮城県"

  79. Oxidative burst as an emergency signal for the active defence in plants against pathogen attacks International conference

    Japan-France Workshop on Signal Transduction in Plants 

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    Event date: 1995.11

    Language:English   Presentation type:Oral presentation (invited, special)  

    Lyon France

  80. Involvement of phospholipases in signal transduction in potato infected with Phytophthora infestans International conference

    "US-Japan Cooperative Science Seminars, Molecular Aspects of Pathogenicity and Resistance: Requirement for Signal Transduction" 

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    Event date: 1995.9

    Language:English   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

    Tsu Japan

  81. The oxidative burst in plants against pathogen attack: aspects, mechanism and role as an emergency signal forplant bio-defence International conference

    Doke, N., Miura, Y., Sanchez, L. M., Park, H.-J., Noritake, T., Yoshioka, H. and Kawakita, K.

    International Conference on Biotechnology Research and Application for Sustainable Development 

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    Event date: 1995.8

    Language:English   Presentation type:Oral presentation (invited, special)  

    Bangkok Thailand

  82. The burst of superoxide generation in active defence response of plants against pathogen attack: mechanism and role International conference

    2nd NIAR/COE Symposium on Defence Response in Plants: from Signal to Gene Expression 

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    Event date: 1995.3

    Language:English   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

    Tsukuba Japan

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Works 7

  1. 植物の「病気撃退法」解明

    2011.3

  2. 病気に強い植物育て

    2011.3

  3. 植物の病原感染防ぐ

    2008.6

  4. 病気に強い植物へ道

    2008.5

  5. 植物の免疫反応を指示ーたんぱく質を特定ー

    2008.5

  6. 疫病耐性イモを解明 GMへの抵抗感、払拭も

    2007.9

  7. ジャガイモ、病気に強く

    2007.6

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Research Project for Joint Research, Competitive Funding, etc. 8

  1. ナノ粒子を用いた農薬送達システムによる革新的植物免疫プライミング技術の開発

    2020.5 - 2023.3

    イノベーション創出強化研究推進事業 

    吉岡博文

      More details

    Grant type:Competitive

  2. バイオセンサーを用いた植物免疫賦与剤のスクリーニング

    2019.6 - 2022.5

    企業からの受託研究 

    吉岡博文

      More details

    Authorship:Principal investigator 

  3. プラントアクチベーター候補剤の病害虫抵抗性試験と作用機構の解明

    2014.11 - 2019.2

    SIP戦略的イノベーション創造プログラム 

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    Grant type:Competitive

  4. イネのウンカ・ヨコバイ類抵抗性遺伝子の単離と抵抗性発現機構の解明

    2013.6 - 2015.2

    農林水産省委託プロジェクト 

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    Grant type:Competitive

  5. 改良型虫害抵抗性遺伝子の導入による耐虫性イネの創出

    2009.7 - 2010.3

    独立行政法人科学技術振興機構 JSTイノベーションプラザ東海 

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    Grant type:Competitive

    シーズ発掘試験

  6. イネのウンカ・ヨコバイ抵抗性遺伝子群の単離と利用

    2008.4 - 2013.3

    新農業展開ゲノムプロジェクト 

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    Grant type:Competitive

  7. 植物免疫シグナル分子を利用した高精度耐病性植物の創生

    2007.7 - 2012.3

    生研センター「新技術・新分野創出のための基礎研究推進事業」 

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    Grant type:Competitive

  8. イネ虫害抵抗性遺伝子の作用機構に関する研究

    2007.4 - 2008.3

    グリーンテクノ計画 

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    Grant type:Competitive

▼display all

KAKENHI (Grants-in-Aid for Scientific Research) 62

  1. 食害昆虫によって誘導される植物免疫シグナルの細胞間移行に関する分子基盤

    2020.7 - 2023.3

    科学研究費補助金  挑戦的研究 (萌芽)

    吉岡博文

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    Authorship:Principal investigator  Grant type:Competitive

    Grant amount:\6500000 ( Direct Cost: \5000000 、 Indirect Cost:\1500000 )

  2. 活性酸素の受容体による柔軟な植物免疫誘導機構の分子基盤

    2020.4 - 2024.3

    科学研究費補助金  基盤研究(B)

    吉岡博文

      More details

    Authorship:Principal investigator  Grant type:Competitive

    Grant amount:\16070000 ( Direct Cost: \11900000 、 Indirect Cost:\4170000 )

  3. Identification of HAMPs from a chewing insect and its roles in plant immunity

    2018.4 - 2020.3

    Grant-in-Aid for Scientific Research  Grant-in-Aid for Publication of Scientific Research Results (Publication of Research Results)

    Yoshioka Hirofumi

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    Authorship:Principal investigator  Grant type:Competitive

    Grant amount:\6240000 ( Direct Cost: \4800000 、 Indirect Cost:\1440000 )

  4. MAPキナーゼセンサーによる植物免疫シグナルの可視化

    2017.4 - 2021.3

    科学研究費補助金  基盤研究(B)

    吉岡博文

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    Authorship:Principal investigator  Grant type:Competitive

    Grant amount:\18720000 ( Direct Cost: \14400000 、 Indirect Cost:\4320000 )

  5. 葉緑体で発生する活性酸素シグナルの調節機構

    2017.4 - 2019.3

    科学研究費補助金  新学術領域研究

    吉岡博文

      More details

    Authorship:Principal investigator  Grant type:Competitive

    Grant amount:\15690000 ( Direct Cost: \12300000 、 Indirect Cost:\3390000 )

  6. NADPHオキシダーゼ活性の時空間的定量法の確立

    2015.4 - 2017.3

    科学研究費補助金  新学術領域研究

    吉岡博文

      More details

    Authorship:Principal investigator  Grant type:Competitive

    Grant amount:\13000000 ( Direct Cost: \10000000 、 Indirect Cost:\3000000 )

  7. MAPキナーゼの基質WRKY型転写因子による植物免疫機構の解明

    2014.4 - 2018.3

    科学研究費補助金  基盤研究(B)

    吉岡博文

      More details

    Authorship:Principal investigator  Grant type:Competitive

    Grant amount:\15990000 ( Direct Cost: \12300000 、 Indirect Cost:\3690000 )

  8. NADPHオキシダーゼ活性の可視化による植物免疫機構の解明

    2013.4 - 2015.3

    科学研究費補助金  挑戦的萌芽研究

    吉岡博文

      More details

    Authorship:Principal investigator  Grant type:Competitive

    Grant amount:\4030000 ( Direct Cost: \3100000 、 Indirect Cost:\930000 )

  9. 植物免疫に関わるMAPキナーゼによる活性酸素の生産制御機構

    2011.4 - 2013.3

    科学研究費補助金  新学術領域研究

    吉岡博文

      More details

    Authorship:Principal investigator  Grant type:Competitive

    Grant amount:\6500000 ( Direct Cost: \5000000 、 Indirect Cost:\1500000 )

  10. 毒素とリシター:病原菌に由来する細胞死誘導因子の機能と病理学的役割の比較研究

    2011.4 - 2014.3

    科学研究費補助金  基盤研究(B)

    児玉基一朗

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    Authorship:Coinvestigator(s)  Grant type:Competitive

    Grant amount:\3900000 ( Direct Cost: \3000000 、 Indirect Cost:\900000 )

  11. 病害応答を制御する葉緑体シグナルの解明

    2010.4 - 2013.3

    科学研究費補助金  基盤研究(B)

    椎名 隆

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    Authorship:Coinvestigator(s)  Grant type:Competitive

    Grant amount:\650000 ( Direct Cost: \500000 、 Indirect Cost:\150000 )

  12. 植物表層における病原糸状菌の分子パターン認識機構とシグナルネットワークの解明

    2009.4 - 2012.3

    科学研究費補助金  基盤研究(B)

    久保康之

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    Authorship:Other 

  13. 毒素vsエリシター:病原菌に由来する細胞死誘導因子の機能と進化に関する比較研 究

    2008.4 - 2011.3

    科学研究費補助金  基盤研究(B)

    児玉基一朗

      More details

    Authorship:Coinvestigator(s) 

  14. 植物の感染防御応答に関わる葉緑体カルシウムシグナル伝達ネットワーク

    2008.4 - 2011.3

    科学研究費補助金 

    中平洋一

      More details

    Authorship:Coinvestigator(s) 

  15. 植物免疫を統御する転写因子の分子機構解明と耐病性化への応用

    2007.4 - 2011.3

    科学研究費補助金  基盤研究(A)

    吉岡博文

      More details

    Authorship:Principal investigator  Grant type:Competitive

    Grant amount:\44850000 ( Direct Cost: \34500000 、 Indirect Cost:\10350000 )

  16. 植物の感染防御応答におけるNOと活性酸素種の協奏的作用機構の解明

    2006.4 - 2008.3

    科学研究費補助金  基盤研究(B)(一般)

    川北一人

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    Authorship:Coinvestigator(s) 

  17. フザリウム病原菌の2種の転写制御因子によって制御される病原性遺伝子群の網羅的同定

    2003.4 - 2007.3

    科学研究費補助金  基盤研究(A)

    柘植尚志

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    Authorship:Coinvestigator(s) 

    科研費

  18. 植物のオキシダティブバーストと生体防御ネットワークの分子生理学的解明と応用

    2002.4 - 2006.3

    科学研究費補助金  基盤研究(S)

    道家紀志

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    Authorship:Coinvestigator(s) 

    科研費

  19. ファイトアレキシン合成系遺伝子のプロモーター解析

    2000.4 - 2002.3

    科学研究費補助金  基盤研究(C)

    吉岡博文

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    Authorship:Principal investigator 

    科研費

  20. ファイトアレキシン合成系遺伝子の構造と機能の解析

    1998.4 - 2000.3

    科学研究費補助金  奨励研究(A)

    吉岡博文

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    Authorship:Principal investigator 

  21. 感染植物のオキシダティブバーストの分子機構と局部・全身的感染防御応答の統御機能

    1999.4 - 2002.3

    科学研究費補助金  基盤研究(A)

    道家紀志

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    Authorship:Coinvestigator(s) 

  22. 植物の新たな感染防御応答因子の探索とその機能解析

    2000.4 - 2003.3

    科学研究費補助金  基盤研究(B)

    川北一人

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    Authorship:Coinvestigator(s) 

    科研費

  23. 生物資源由来物質による植物免疫の誘導とその病害防除への応用試験

    1998.4 - 2000.3

    科学研究費補助金  基盤研究(B)(2)(展開)

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    Authorship:Coinvestigator(s) 

  24. 植物の感染防御応答へのシグナル機能をもつオキシダティブバーストの分子生理機構

    1997.4 - 1999.12

    科学研究費補助金  基盤研究(B)

    道家紀志

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    Authorship:Coinvestigator(s) 

    科研費

  25. 植物のストレス応答における新規な全身的シグナル伝達系の解析

    1997.4 - 1999.3

    科学研究費補助金  萌芽的研究・

    道家紀志

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    Authorship:Coinvestigator(s) 

  26. ジャガイモ塊茎組織におけるファイトアレキシン合成系酵素の活性化制御機構

    1993.4 - 1994.3

    科学研究費補助金  奨励研究(A)

    吉岡博文

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    Authorship:Principal investigator 

  27. ジャガイモ塊茎組織におけるファイトアレキシン合成系遺伝子の発現制御

    1992.4 - 1993.3

    科学研究費補助金  奨励研究(A)

    吉岡博文

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    Authorship:Principal investigator 

  28. Molecular basis for cell-to-cell transfer of plant immune signals induced by feeding insects

    Grant number:20K21310  2020.7 - 2023.3

    Grants-in-Aid for Scientific Research  Grant-in-Aid for Challenging Research (Exploratory)

    Hirofumi Yoshioka

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    Authorship:Principal investigator 

    Grant amount:\6500000 ( Direct Cost: \5000000 、 Indirect Cost:\1500000 )

    Spatio-temporal activation of MAPKs in neighboring cells by 28-spotted ladybug feeding damage could be mimicked by the simultaneous treatment with injury and HAMPs. Using this system, it is possible to search for potato blight pathogen effectors that inhibit MAPK activation in neighboring cells. Nicotiana benthamiana leaves expressing effectors and cytoplasm-localized MAPK sensors were treated with HAMP and injury, then, diffusion of FRET fluorescence from the treated area to surrounding cells was observed. On the other hand, when various effectors were introduced, the diffusion of FRET fluorescence was suppressed in the plots expressing five effectors.

  29. ナノ粒子を用いた農薬送達システムによる革新的植物免疫プライミング技術の開発

    2020.5 - 2023.3

    生物系特定産業技術研究支援センター  イノベーション創出強化研究推進事業 

    吉岡博文

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    Grant type:Competitive

  30. 活性酸素の受容体による柔軟な植物免疫誘導機構の分子基盤

    Grant number:20H02984  2020.4 - 2024.3

    科学研究費助成事業  基盤研究(B)

    吉岡 博文

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    Authorship:Principal investigator 

    Grant amount:\18070000 ( Direct Cost: \13900000 、 Indirect Cost:\4170000 )

    植物免疫応答では、原形質膜に存在するNADPHオキシダーゼによって2相性のROSバーストが誘導される。ROSバーストは第一では弱く、第二ではより激しく起こり、免疫細胞死に重要な役割を果たす。ROSはそのセンサータンパク質を酸化することによって構造を変化させ、様々な細胞応答を引き起こすと考えられている。しかし、植物免疫応答に関与するROSセンサーについては未開拓である。本研究では、植物免疫応答の鍵を握るROSセンサータンパク質を網羅的に探索し、機能を明らかにすることでROSによる免疫統御機構を明らかにすることを目的とする。
    本研究の目的は、植物免疫応答の鍵を握るROSセンサータンパク質を網羅的に探索し、機能を調べることによってROSによる免疫統御機構を明らかにすることである。ROSは、世界中で防御応答のマーカーとして観察されているが、その作用機構について説明することができない。ROSであるH2O2は、そのセンサータンパク質のシステインのチオール基 (-SH) を酸化し (-SOH;スルフェン酸)、分子内または分子間でジスルフィド結合を形成する。あるいは、スルフェン酸が還元型グルタチオン (GSH) と反応してS-グルタチオン化 (-S-SG) することによってROSセンサータンパク質の構造を変化させ、様々な細胞応答を引き起こすことが知られている。
    これまでに、PTIおよびETIにおいてスルフェニル化されると予想される多くのタンパク質を得ることができた。これらの中で、カルシウムチャネルと予想される候補タンパク質を大腸菌で発現させ、リコンビナントタンパク質を得た。リコンビナントタンパク質を過酸化水素によって酸化させ、スルフェン酸と特異的に結合するdimedoneを加えた後、抗システイン-dimedone抗体を用いてウエスタン解析した。その結果、候補タンパク質がin vitroでスルフェニル化されることを確認した。本年度は、さらに、標的システインをアラニンに置換した変異リコンビナントタンパク質は、スルフェニル化されないことを確認した。
    ベンサミアナタバコ葉において候補遺伝子を抑制し、病原菌に対する影響を調べた。その結果、ジャガイモ疫病菌および灰色かび病菌が著しく感染するようになった。さらに、ベンサミアナタバコ葉に発現させ、病害シグナルを与えた後に免疫沈降することで標的タンパク質を回収し、in vivoでスルフェニル化されることを確かめた。しかし、現時点ではLCMS/MS解析の結果は得られていない。
    ROSは、植物免疫応答を局部的、全身的あるいはPTIおよびETIにおいて重要なシグナル分子であると認識され、世界中で防御応答のマーカーとして観察されているが、その作用機構について説明することができない。ROSであるH2O2は、そのセンサータンパク質のシステインのチオール基 (-SH) を酸化し (-SOH;スルフェン酸)、分子内または分子間でジスルフィド結合 (-S-S-) を形成する。あるいは、スルフェン酸が還元型グルタチオン (GSH) と反応してS-グルタチオン化 (-S-SG) することによってROSセンサータンパク質の構造を変化させ、様々な細胞応答を引き起こすことが知られている。
    これまでに、YAP1を用いてROSセンサーを網羅的に探索し、PTIおよびETIにおいてスルフェニル化されると予想される多くのタンパク質を得ることができた。これらの中で、カルシウムチャネルと予想される候補タンパク質を大腸菌で発現させ、リコンビナントタンパク質を得た。リコンビナントタンパク質を過酸化水素によって酸化させ、スルフェン酸と特異的に結合するdimedoneを加えた後、抗システイン-dimedone抗体を用いてウエスタン解析した。その結果、候補タンパク質がin vitroでスルフェニル化されることを確認した。
    本年度は、ベンサミアナタバコ葉において候補遺伝子を抑制し、病原菌に対する影響を調べた。その結果、ジャガイモ疫病菌および灰色かび病菌が著しく感染することが確かめられた。さらに、候補遺伝子を抑制したベンサミアナタバコにおいて、スルフェニル化されるシステインを置換した変異候補遺伝子では、灰色かび病菌に対する抵抗性を相補することができなかった。この結果は、標的システインが抵抗性発現において重要な役割を果たすことを示している。以上のように、おおむね予定通り研究が進展した。
    これまでに、カルシウムチャネルと予想される候補遺伝子のコンビナントタンパク質を過酸化水素によって酸化させ、スルフェン酸と特異的に結合するdimedoneを加えた後、抗システイン-dimedone抗体を用いてウエスタン解析した。その結果、候補タンパク質がin vitroでスルフェニル化されることを確認した。さらに、得られたROSセンサーのスルフェニル化の機能を解析する目的で、dimedoneより強くスルフェニル化されたシステインと結合するBTD (benzothiazine-based probe) で化学ラベルし、コンビナントタンパク質が過酸化水素によって酸化されることを確認した。また、ベンサミアナタバコ葉に候補遺伝子を発現させ、病害シグナルを与えた後に免疫沈降することで標的タンパク質を回収し、in vivoでスルフェニル化されることを確かめた。しかし、現時点ではLCMS/MS解析の結果は得られていない。
    本年度は、dimedoneに比べて100倍以上スルフェニル化されたシステインとの反応性が高いBTDを用いてin vivoにおける候補タンパク質のスルフェニル化を調べる。タグを付加した候補遺伝子をベンサミアナタバコ葉で発現させ、病害シグナルを与えた後に免疫沈降することで標的タンパク質を回収し、in vivoでスルフェニル化されることを確かめる予定である。

  31. バイオセンサーを用いた植物免疫賦与剤のスクリーニング

    2019.6 - 2020.5

    住友化学株式会社  企業からの受託研究 

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    Grant type:Competitive

  32. Identification of HAMPs from a chewing insect and its roles in plant immunity

    Grant number:18K19210  2018.6 - 2020.3

    Grants-in-Aid for Scientific Research  Grant-in-Aid for Challenging Research (Exploratory)

    Yoshioka Hirofumi

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    Authorship:Principal investigator 

    Grant amount:\6240000 ( Direct Cost: \4800000 、 Indirect Cost:\1440000 )

    In this research project, the primary purpose of this study is to purify HAMP (herbivore-associated molecular pattern) from oral secession from Henosepilachna vigintioctopunctata and to determine its structure. We found that there is a substance that induces ROS production in the oral secession of the ladybird. The ROS-inducing active fraction was purified and collected from a large amount of the oral secession using various columns. As a result of LC-MS / MS analysis of the produced active ingredient, it was found that the compound had a molecular weight of 1,000 or less, and that treatment of Nicotiana benthamiana leaves with this fraction transiently induced ROS production and MAPK activity.

  33. Visualization of MAP kinase activity by a biosensor in plant immune responses

    Grant number:17H03772  2017.4 - 2021.3

    Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

    Yoshioka Hirofumi

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    Authorship:Principal investigator 

    Grant amount:\18720000 ( Direct Cost: \14400000 、 Indirect Cost:\4320000 )

    In plant immunity, there are two sequential immune responses, PTI (Pattern-triggered immunity) and ETI (Effector-triggered immunity). In both cases, MAP kinases play an important role in the immune responses. The applicant has started the development of a biosensor that spatiotemporally monitors MAP kinase activity at the cell and tissue levels. The purpose of this study is to understand the actual state of population-coordinated biological defense mechanism, and by visualizing MAP kinase activity in response to pathogen infection, it is possible to discover new signal networks in PTI and ETI, presenting a pioneering model system.

  34. 葉緑体で発生する活性酸素シグナルの調節機構

    Grant number:17H05526  2017.4 - 2019.3

    科学研究費助成事業  新学術領域研究(研究領域提案型)

    吉岡 博文

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    Authorship:Principal investigator 

    Grant amount:\14690000 ( Direct Cost: \11300000 、 Indirect Cost:\3390000 )

    植物の免疫応答では、葉緑体における活性酸素種 (ROS) の生成が誘導されることが知られている。光化学系II (PSII) では一重項酸素が、PSIではO2-が生成される。レトログレードシグナルとしてO2-から派生したH2O2が葉緑体から核へ直接送り込まれる可能性が示されている。葉緑体のH2O2は、葉緑体または核でROSセンサー分子に受容されると予想される。本研究では、葉緑体のROSを介したシグナル伝達機構の解明を目指し、免疫応答、PTI (pattern-triggered immunity) および細胞死を伴うETI (effector-triggered immunity) におけるROSセンサー分子の役割を調べた。
    ROSを介した主なシグナル伝達は、センサー分子のシステイン残基がH2O2と反応してスルフェン酸を形成し、スルフェン酸が修飾を受けることでシグナルが誘導される。スルフェン酸と反応してシグナル伝達を阻害するYAP1を葉緑体に発現させたところ、INF1誘導による細胞死が抑制された。ベンサミアナタバコにPTIとETIをそれぞれ誘導し、クロロフィル蛍光とガス交換速度の測定により光合成活性測定を行った。ETIを誘導するRpi-blb2/AVRblb2は、PSI に連動するCO2固定活性を顕著に抑制した。一方で、PTIを誘導するflg22はCO2固定に影響を与えなかった。さらに、PTIとETIは共にPSII活性に影響を与えなかった。以上の結果から、ETIにおけるCO2固定阻害に起因した過剰なNADPHが、葉緑体でのROS生成に寄与しているものと考えられた。
    以上より、葉緑体のROSシグナルは、細胞死を誘導する因子に関連した複雑なネットワークを形成しているものと思われた。
    平成30年度が最終年度であるため、記入しない。
    平成30年度が最終年度であるため、記入しない。

  35. NADPHオキシダーゼ活性の時空間的定量法の確立

    Grant number:15H01398  2015.4 - 2017.3

    科学研究費助成事業  新学術領域研究(研究領域提案型)

    吉岡 博文

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    Authorship:Principal investigator 

    Grant amount:\13000000 ( Direct Cost: \10000000 、 Indirect Cost:\3000000 )

    ベンサミアナタバコ (Nicotiana benthamiana) においては、NbRBOHBが病害応答性CDPKやRLCKによってリン酸化され、ROS生産を誘導する。本年度は、2種の蛍光タンパク質 (YFP、CFP)、NbRBOHB由来のリン酸化配列を用いて、CDPKまたはRLCKによるリン酸化によって生ずる構造の変化に伴って蛍光共鳴エネルギー移動 (Foster Resonance Energy Transfer: FRET) が起こる2つのセンサーの基本構造を構築し、FRET効率の最適化を図った。
    FRETシステムは、センサーのリン酸化時と非リン酸化時で異なる波長の蛍光として観察されるため、CDPKまたはRLCKの活性化を生細胞で蛍光として観察できる。2遺伝子発現型バイナリーベクターをベースとして、作製したセンサーとエフェクターをそれぞれ35Sプロモーターの下流に挿入した。エフェクターには、病害応答性CDPKの恒常活性型変異体 (NbCDPK5VK) および非活性型変異体 (NbCDPK5VK-KM) を用いた。これらバイナリーベクターを、アグロインフィルタレーション法によってベンサミアナタバコに導入して発現させ、葉組織から粗抽出液を調製し、マルチプレートリーダーを用いてFRET蛍光 (YFP/CFP) の強度を評価した。しかし、現在までに良好なFRET蛍光は得られていない。そこで、NbRBOHB遺伝子の転写を制御するWRKY8転写因子を同定し、この転写因子を活性化するMAPKの活性化を可視化することを可能にしたMAPKバイオセンサーの開発に成功した。このセンサーをベンサミアナタバコに一過的に導入して発現させた結果、病原菌の感染シグナルに応答したFRET蛍光が観察された。
    28年度が最終年度であるため、記入しない。
    28年度が最終年度であるため、記入しない。

  36. プラントアクチベーター候補剤の病害虫抵抗性試験と作用機構の解明

    2014.11 - 2019.2

    農林水産省  SIP戦略的イノベーション創造プログラム 

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    Grant type:Competitive

  37. Mechanism for plant immune responses by WRKY transcription factors, physiological substrates of MAPKs

    Grant number:26292023  2014.4 - 2017.3

    Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

    Yoshioka Hirofumi

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    Authorship:Principal investigator 

    Grant amount:\13650000 ( Direct Cost: \10500000 、 Indirect Cost:\3150000 )

    Multiple WRKY transcription factors function in plant immunity through phosphorylation by MAPKs. Here, we show that plant immune WRKYs downregulate the large number of photosynthesis-related genes and inhibit photosynthetic CO2 fixation. This inhibition results in chloroplastic reactive oxygen species (ROS) burst in Nicotiana benthamiana epidermal cells. Sensing of Phytophthora infestans effector by a cognate receptor also induces the WRKY-dependent chloroplastic ROS burst in epidermal cells, conferring resistance to invasion of P. infestans into epidermal cells. These results suggest that the MAPK-WRKY pathway triggers epidermal cell-specific chloroplastic ROS burst to prevent pathogen invasion in N. benthamiana leaves.

  38. イネのウンカ・ヨコバイ類抵抗性遺伝子の単離と抵抗性発現機構の解明

    2013.6 - 2015.2

    農林水産省  農林水産省委託プロジェクト 

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    Grant type:Competitive

  39. Visualization of NADPH oxidase activity in plant immunity

    Grant number:25660293  2013.4 - 2015.3

    Grants-in-Aid for Scientific Research  Grant-in-Aid for Challenging Exploratory Research

    YOSHIOKA Hirofumi

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    Authorship:Principal investigator 

    Grant amount:\4030000 ( Direct Cost: \3100000 、 Indirect Cost:\930000 )

    Reactive oxygen species (ROS) are important signaling molecules in plant immunity. Plant NADPH oxidase is termed as respiratory burst oxidase homologue (RBOH). Previously, we found that a calcium-dependent protein kinase (CDPK) activates RBOH by direct phosphorylation of the N-terminal region. Here, we tried to optimize a biosensor to patio-temporally monitor phosphorylation of the RBOH by CDPKs. However, we could not obtain fine construct of the sensor to generate enough intensity of fluorescence based on fluorescence resonance energy transfer (FRET).

  40. Toxin and elicitor: a comparative analysis of functions and pathological roles of cell death inducers from plant pathogenic fungi

    Grant number:23380025  2011.4 - 2014.3

    Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

    KODAMA Motoichiro, YOSHIOKA Hirofumi, KAMINAKA Hironori

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    Authorship:Coinvestigator(s) 

    Toxins and elicitors from plant pathogens appear to be functional as virulence and avirulence factors, respectively. While, both factors have a common role as an inducer for programmed cell death in plant cells. In the project, we showed evidence that cell death in a host plant induced by both host-specific toxins and elicitors leads to the compatible reaction in the infection process of necrotrophic plant pathogen. Both the toxin and the elicitor might act as pathogenicity factors in host-necrotrophic pathogen interactions. In addition, the results indicate that cell death cascades triggered by those factors might be mediated by different signaling pathways induced by each factor.

  41. 植物免疫に関わるMAPキナーゼによる活性酸素の生産制御機構

    Grant number:23117707  2011.4 - 2013.3

    科学研究費助成事業  新学術領域研究(研究領域提案型)

    吉岡 博文

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    Authorship:Principal investigator 

    Grant amount:\6500000 ( Direct Cost: \5000000 、 Indirect Cost:\1500000 )

    植物は病原菌の攻撃に応答して急激な活性酸素種の生成を引き起こす。この反応はROSバーストと呼ばれ、植物の抵抗反応や過敏感細胞死において重要な役割を果たす。ROSバーストは、主に細胞膜に存在するNADPHオキシダーゼであるRBOHに依存して引き起こされる。ジャガイモ葉組織においては、StRBOHCがジャガイモ葉組織での病害応答に関与することが示されている。葉組織におけるROSバーストに至るまでのシグナル伝達経路の解明は、植物の防御応答システムを明らかにする上で重要である。本研究では、StRBOHCのベンサミアナタバコにおけるオルソログであるNbRBOHBの転写誘導機構を探ることにより、MAPキナーゼカスケード (MEK2-SIPK) がROSバーストを制御する機構を明らかにすることを目的とする。
    平成24年度は、NbRBOHBプロモーターのMEK2-SIPKに応答するシス配列に結合する転写因子を決定した。これまでに、StRBOHCおよび NbRBOHBプロモーターの転写因子としてW-box配列を含む推定シス配列の11 bp (11W) を同定した。そこで、MAPKの基質として得られているWRKY型転写因子のNbRBOHBプロモーター活性に及ぼす影響について調べた。酵母ワンハイブリッド解析によって、これら基質の中で11Wと結合する4つのWRKY型転写因子が得られた。さらに、11Wを3タンデムに連結した配列を35Sの最小プロモーターに結合したキメラプロモーターは、11Wと結合したWRKY型転写因子により誘導された。これらWRKY遺伝子をノックダウンすると、エリシター誘導によるNbRBOHBの転写が抑制された。以上の結果より、MAPKの基質である複数のWRKY型転写因子は、11Wに結合することでStRBOHCおよび NbRBOHBプロモーターを活性化するものと思われた。
    24年度が最終年度であるため、記入しない。
    24年度が最終年度であるため、記入しない。

  42. ChloroplastSignalsregulatingplantimmuneresponses

    Grant number:22370022  2010 - 2012

    Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

    SHIINA Takashi, YOSHIOKA Hirofumi

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    Salicylic acid acts as an endogenous signal that triggers local and systemic response in the plant immune system. Both reactive oxygen species (ROS) and nitric oxide (NO) may play important roles in regulation of immune cell signal transductions. It should be noted that chloroplasts are involved in the production of theses immune signals. However, a role for chloroplasts in plant immune system remains largely elusive. This study provides novel insights into chloroplastic control of plant immunity through a thylakoid-localized Ca2+ binding protein CAS.

  43. 改良型虫害抵抗性遺伝子の導入による耐虫性イネの創出

    2009.7 - 2010.3

    独立行政法人科学技術振興機構 JSTイノベーションプラザ東海  独立行政法人科学技術振興機構 JSTイノベーションプラザ東海 

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    Grant type:Competitive

    シーズ発掘試験

  44. Analysis on the recognition mechanisms of molecular pattern of plant pathogenic fungi and signaling network

    Grant number:21380031  2009 - 2011

    Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

    KUBO Yasuyuki, MORI Masashi, NISHIUCHI Takumi, KOGA Hironori, YOSHIOKA Hirofumi

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    Authorship:Collaborating Investigator(s) (not designated on Grant-in-Aid) 

    In this research project, to elucidate plant basal resistance mechanisms against plant pathogenic fungi, modification of cell surface structure of pathogen and its involvement of recognition and signaling network leading to plant defense response was analyzed. Especially, using rice-rice blast fungus and Arabidopsis thaliana. anthracnose fungus system, signaling network downstream of pathogen recognition was extensively analyzed based on genome based approach. Manipulation of pivotal transcription factor involving disease resistance enabled us to generate disease resistant transgenic rice plants.

  45. イネのウンカ・ヨコバイ抵抗性遺伝子群の単離と利用

    2008.4 - 2013.3

    農林水産省  新農業展開ゲノムプロジェクト 

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    Grant type:Competitive

  46. Calcium signal transduction network in plant innate immunity

    Grant number:20200060  2008 - 2010

    Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research on Innovative Areas (Research a proposed research project)

    NAKAHIRA Yoichi, YUKAWA Yasushi, SHIINA Takashi, YOSHIOKA Hirofumi, SUGIURA Masahiro, TOZAWA Yuzuru, KUCHITU Kazuyuki

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    Authorship:Collaborating Investigator(s) (not designated on Grant-in-Aid) 

    Mitochondria and chloroplasts are not only energy source for eukaryotic cells, but also play additional important roles to modulate cellular functions. For example, mammal mitochondria contribute to the initiation of apoptosis, diseases and aging. Plant chloroplast also serves several important functions in plant immunity, including biosynthesis of salicylic acid (SA) and the generation of reactive oxygen species (ROS), key signaling molecules in plant immune response. Interestingly, previous studies have shown that chloroplasts contain proteins involved in plant immune responses. Furthermore, several pathogen effectors target chloroplasts to suppress chloroplast-derived defense signals. Therefore, it is anticipated that chloroplasts play a role in plant innate immunity. However, how chloroplasts sense pathogen signals and control immune signaling network remains unclear. This study illustrates a previously unknown crosstalk between chloroplasts and innate immunity through chloroplast Ca^<2+> signaling in plants.
    Mitochondrial Ca^<2+> homeostasis plays a key role in apoptosis by switching Ca^<2+>-induced release of cytochrome C (caspase cofactor). However, very little is known about Ca^<2+> homeostasis in chloroplasts. Here, we report that pathogen associated molecular pattern (PAMP) signals induce a rapid increase in the stromal Ca^<2+> concentration. Pharmacological studies demonstrate that the cytoplasmic Ca^<2+> signal is transferred to the chloroplasts and induces the stromal Ca^<2+> transient. We further demonstrate that chloroplast-localized putative Ca^<2+> binding protein, CAS is involved in the generation of stromal Ca^<2+> signals. These studies suggest a novel mechanism relaying PAMP signals into chloroplasts at the beginning of the immune process.
    The PAMP-induced stromal Ca^<2+> signaling may control or activate the innate immune response. Thus, we examined the role of CAS in plant innate immunity. Remarkably, CAS deficient plants (cas-1) are compromised in broad range of innate immune responses including PAMP-induced basal defense and R-gene mediated hypersensitive cell death. It is unlikely that the compromised immunity of cas-1 plants is caused by limited cellular resources, since CAS deficient mutant lines exhibit normal phenotype and photosynthetically competent. Furthermore, a comprehensive analysis of plant hormone dynamics demonstrated that CAS is specifically required for PAMP-induced accumulation of SA, a key signal molecule in plant immune responses. These results suggest that chloroplast protein CAS acts as a positive regulator of plant innate immunity through SA-dependent signaling.
    CAS regulates SA biosynthesis most likely at transcriptional level. It is therefore anticipated that chloroplasts release retrograde signals that control defense gene expression during immune responses. PAMP induces expression of numerous defense related genes in nucleus. Using a genome wide transcriptome analysis we show that CAS is indispensable for the global induction of defense gene expression before SA biosynthesis starts, suggesting that CAS is involved in the chloroplast-to-nucleus retrograde signaling and mediates chloroplast control of plant innate immunity. Chloroplast-derived ^1O_2^- signaling has been implicated with the control of stress defense-related gene expression. Here we show that most genes downregulated in CAS deficient plants were enriched for ^1O_2^- responsive genes, providing evidence for a role of CAS in ^1O_2^- signaling to control nuclear-encoded defense genes.
    Collectively this study illustrates previously unknown mechanisms that coordinate chloroplast functions with nuclear-encoded defense gene expression. Upon infection, PAMP signals are relayed to chloroplasts through the cytoplasmic Ca^<2+>-induced stromal Ca^<2+> signaling pathway. This study further implicates a role of ^1O_2^- retrograde signaling as underlying mechanisms of chloroplast control of plant innate immunity. Chloroplast protein CAS plays a critical role in both processes. Identification of the chloroplast-dependent immune pathway may provide a novel strategy for the disease control of plants.

  47. Toxin vs. elicitor : a comparative analysis of function and evolution of cell death inducers from plant pathogenic fungi

    Grant number:20380028  2008 - 2010

    Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

    KODAMA Motoichiro, YOSHIOKA Hirofumi

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    Toxins and elicitors from plant pathogens appear to function as virulence and avirulence factors, respectively. While, both factors have a common role as an inducer for programmed cell death in plant cells. Here we provide evidence that cell death in a host plant induced by both host-specific AAL-toxin and INF1 elicitor leads to the compatible reaction in the infection process of necrotrophic plant pathogen Alternaria alternate. Both the toxin and the elicitor might act as pathogenicity factors in host-necrotrophic pathogen interactions.

  48. 植物免疫シグナル分子を利用した高精度耐病性植物の創生

    2007.7 - 2012.3

    独・農業・食品産業技術総合研究機構  生研センター「新技術・新分野創出のための基礎研究推進事業」 

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    Grant type:Competitive

  49. イネ虫害抵抗性遺伝子の作用機構に関する研究

    2007.4 - 2008.3

    農林水産省  グリーンテクノ計画 

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    Grant type:Competitive

  50. Molecular mechanism of transcription factor controlling plant immunity and its application in the production of pathogen-resistant plants.

    Grant number:19208004  2007 - 2010

    Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (A)

    YOSHIOKA Hirofumi

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    Authorship:Principal investigator 

    Grant amount:\44850000 ( Direct Cost: \34500000 、 Indirect Cost:\10350000 )

    Mitogen-activated protein kinase (MAPK) cascades have pivotal roles in plant innate immunity. However, downstream signaling of plant defense-related MAPKs is not well understood. Here we provide evidence that Nicotiana benthamiana WRKY8 is a physiological substrate of SIPK, NTF4, and WIPK. Phosphorylation of WRKY8 increased its DNA-binding activity. MAPK-mediated phosphorylation of WRKY8 has an important role in the defense response through activation of downstream genes.

  51. Harmonious mode of NO and reactive oxygen species action in defense responses in plants

    Grant number:18380032  2006 - 2008

    Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

    KAWKAKITA Kazuhito, TAKEMOTO Daigo, SONODA Masatoshi

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    Authorship:Coinvestigator(s) 

  52. Identification of pathogenicity genes regulated by two kinds of transcription regulators of the fungal plant pathogen Fusarium oxysporum

    Grant number:15208005  2003 - 2006

    Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (A)

    TSUGE Takashi, YOSHIOKA Hirofumi

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    We previously identified two genes, FOW2 and FOW3, which encode Zn(II)2Cys6-type and His2Cys2-type transcription regulators, respectively, essential for plant infection in the melon wilt pathogen Fusarium oxysporum f. sp. melonis. In this research, we attempted to isolate Fow2-or Fow3-regulated genes.
    We constructed cDNA subtraction libraries, which contained genes with changes in transcription levels caused by mutations in FOW2 and FOW3. Screening of these libraries by cDNA dot-blot differential hybridization and RNA gel blot analyses identified six genes positively regulated by Fow2. We made mutants of these genes and identified a Ren1-regulated pathogenicity gene, named VRF2-1. The vrf2-1 mutant showed reduced virulence on melon plants, although the fow2 mutant completely lost pathogenicity. This result indicates that Fow2 regulates the transcription of multiple pathogenicity genes, including VRF2-1. We sequenced a BAC clone encoding FOW2 and found that VRF2-1 is also encoded by this clone. This clone contains additional 29 genes, of which 10 were found to be possibly regulated by Ren1, suggesting that the Fow2-regulated genes are closely linked with FOW2. Database homology search and PCR analysis revealed that the filamentous ascomycetes species have the FOW2 homologues. This suggests that the FOW2 homologues also control plant infection in other fungi.
    We also identified two genes, REN1 and FoSTUA, which encode different types of transcription regulators essential for conidiation in F. oxysporum. In plant pathogenic fungi, conidia play important roles in the disease cycle as disseminated propagules and primary and secondary inocula. We performed expressed sequence tag analysis during vegetative growth and conidiation and identified 496 genes specifically detected in the conidiation cDNA library. Comparison of the transcription levels of the 496 genes between the wild-type strain and the ren1 mutant or the fostuA mutant identified 35 and three genes regulated by Ren1 and FoStuA, respectively.

  53. Molecular and physiological studies on oxidative burst and active defense mechanism in plant and its application for disease tolerance

    Grant number:14104004  2002 - 2005

    Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (S)

    DOKE Noriyuki, KAWAKITA Kazuhito, YOSHIOKA Hirofumi

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    This project made it clear the followings on molecular mechanisms of oxidative burst (OXB) and active defense-network in Phytophtora infestans-infected potato plants. 1)In the course of the defense response, immediately-responsive OXB and induced OXB, which were dependent on superoxide-generating NADPH oxidase, RbohA and RbohB, were induced and then played a key signal role in the induction of HR-type defense response. 2)RbohA was activated through Ca^<2+> influx and a Ca^<2+>-dependent protein kinase, whereas RbohB was induced under control of MAPK cascade, and then activated by CDPK in relation to the 1st OXB. 3)A transient expression of MEK1^<DD> of MAPKK, that was modified by amino acid exchange to be constitutively active, caused activation of SIPK and WIPK of MAPK, induction of RbohB, HMGR,PVS etc. and then induction of HR-type defense response. 4)Activated MPK1 phosphorylated multiple host proteins (PPS1-8),PPS3 of which regulated expressions of RbohB, HMGR, and PVS. 5)Compatible races also induced activation of MAPK cascade and induction of non-active RbohB,HMGR and PVS, and stimulate infection-specific GUS expression in transgenic potato with PVS3 promoter-linked GUS gene. 6)NO generation was found to be accompanied by the 1st OXB, playing an important role in induction of HR-type response. 7)The NO generation was dependent on inducible-nitrate reductase (NR) and other unknown enzyme. 8)Local OXB stimulated a sequential Ca^<2+> influx among inner tissue cells, and this signal came to stimulate systemic OXB in the surface tissue, following expression of RbohB and PAL for induction of systemic acquired resistance (SAR). 9)Transgenic potato plants with PVS3 promoter-linked MEK^<DD> demonstrated tolerance to virulent P.infestans and Alternaria solani with HR-type defense response.

  54. Investigation of novel plant defense-related genes and the functional analysis

    Grant number:12460022  2000 - 2002

    Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

    KAWAKITA Kazuhiro, YOSHIOKA Hirofumi, DOKE Noriyuki

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    The aim of this study is to screen novel resistance-related genes and analyze the function of these genes in plant defense responses against pathogen attack. We employed differential hybridization, subtraction and differential display methods to isolate elicitor-inducible genes of tobacco and potato using the plant-fungal elicitor from Phytophthora infestans system. Sequence analysis of cDNAs identified by differential hybridization in tobacco revealed similarities to genes for SAR8.2, glycine-rich protein, extensin, acyltransferase. Sequence analysis of cDNAs identified by subtraction in potato revealed similarities to genes for pathogenesis-related (PR) genes, glutathione-S-transferase, peroxidase, catalase, 1-aminocylopropane-1-carboxylic acid oxidase, and wound-inducible genes. Sequence analysis of cDNAs identified by differential hybridization in potato revealed similarities to genes for tyramine hydroxycinnamoyl transferase, glucose 6-phosphate dehydrogenase, pyruvate decarboxylse, and defense related-genes. We analyzed the function of two novel genes isolated by differential display in tobacco. One is elicitor-inducible cytochrome P450 gene designated CYP82E1. There is the possibility that CYP82E1 act in biosynthesis of barrier or antifungal compounds to limit invasion of pathogens. The other gene codes for a leucine-rich repeat (LRR) protein closely related to Cf genes in tomato and designated EILP (Elicitor-Inducible LRR Protein) gene. In transgenic tobacco plants overexpressing EILP, expression of defense-related genes against various elicitors increased and resistance against pathogenic bacteria was activated, implying that the product of EILP acts as a receptor-like protein amplifying signals in plants infected with pathogen and elicit defense responses.

  55. Functional Analysis of the Promoter for Phytoalexin Synthesis Gene

    Grant number:12660043  2000 - 2001

    Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    YOSHIOKA Hirofumi

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    Authorship:Principal investigator 

    Grant amount:\3400000 ( Direct Cost: \3400000 )

    Exposure of potato tuber tissues to incompatible race of Phytophthora infestans, a pathogen of potato late blight, causes multiple defense responses, including the oxidative burst, accumulation of sesquiterpenoid phytoalexins (lubimin and rishitin). All these reactions are also induced in potato tubers by the treatment with a crude elicitor preparation (hyphal wall component ; HWC) from the mycelium of the fungus. Sesquiterpene cyclase is a key enzyme in isoprenoid pathway for the synthesis of sesquiterpenoid phytoalexin. The expression of the gene in plant-pathogen interactions is not well documented. Here we isolated and characterized CDNA encoding potato sesquiterpene cyclase, vetispiradiene synthase (PVS). PVS was encoded by multiple gene family (PVS1-PVS4). As one molecular approach to elucidation of the mechanisms that regulate the phytoalexin synthesis in potato tubers, we investigated changes with time in the transcript levels of PVS. These members were stimulated by an incompatible and a compatible races of P. infestans. Interestingly, only PVS3 was induced in the potato leaves.
    We isolated and characterized the PVS3 genes from a potato genomic library.Transient expression of luciferase (Luc) reporter gene under the control of PVS3 promoter showed that high level of Luc activity was induced by the fungal elicitor. These results indicated that the promoter region contains cis-acting element for defense response.

  56. Molecular mechanism or oxidative burst in infected plants and its control mechanism of networks of local nd systemic defense response to infection

    Grant number:11306004  1999 - 2001

    Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (A)

    DOKE Noriyuki, YOSHIOKA Hirofumi, KAWAKITA Kazuhiko

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    Molecular basis of oxidative burst (OXB) system, which was an initial signal for induction of defense response in plants, regulatory mechanisms for activation of the system, and dynamic phases of molecular and physiological changes associated with local or systemic induced resistance were investigated for understanding of a systematic mechanism of plant active defense under the control by oxidative burst Following results were obtained :
    1 ) Two gp91phox horologes, which may be a subunit of NADPH oxidase, were cloned from potato and tobacco plants, respectively, and they were characterized by membrane fern proteins with binding sites of NADPH and calcium,
    2 ) The OXB system was rapidly activated from inactive state by wounding or infection stimuli and OXB in the potentiated cells was inhibited by Ca^<2+> channel blockers, calmodulin inhibitors or protein kinase inhibitors, suggesting involvement of a C kinase in induction of OXB.
    3 ) Defense-metabolism-related genes, which were stimulated by OXB-inducible elicitors, were cloned by mean of differential display or subtraction,
    4 ) Gene silencing about some of the cloned genes by using a PVX vector demonstrated that genes responsible for OXB or NADPH producer were a crucial role in defense expression against incompatible Phytophthora fungus.
    5 ) Induction of local OXB caused local as well as systemic induced resistance, dispatching a noble systemic signal which caused a sequential Ca^<2+> influx from cells to cells in non potentiated tissues and then induced systemic OXB at the potentiated tissue for triggering induction of systemic acquired resistance.
    6 ) A positive regulation of induction of OXB system by signal dispatching elements was suggested to be applicable for induction of plant immunization against pathogens.

  57. Induction of plant immunization by components of bio-resources and its application for screening of inducers.

    Grant number:10556010  1998 - 2000

    Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B).

    DOKE Noriyuki, YOSHIOKA Hirohumi, KAWAKITA Kazuhito

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    Authorship:Coinvestigator(s) 

    It is known that plants acquire induced resistance around firstly attacked tissue as well as at remote tissues area through a systemic signaling. An observation of systemic oxidative burst in plants which were partly stimulated by elicitor with activity to induce local OXB and systemic acquired resistance (SAR) had made us to plan this projects. The objective of this projects was to analyze the physiological mechanism of dispatching of systemic signals and signal transduction from cells to cells for activation of the systemic OXB which may become a induction signal for SAR.
    Results are as follows : 1. A fungal elicitor and known chemical activators of SAR were confirmed to induce systemic OXB and SAR.2. There was two ways to dispatch the systemic signal for induction of systemic OXB.One was through activation of local OXB and generated hydrogen peroxide in a potentiated tissue cells treated with fungal elicitor, the other through activation of non-potentiated inside cells treated with stimulators such as salicylic acid and plant activators. 3. In the tissue-cells where systemic signals were translated, a chain response of cells with calcium influx and pH increase was monitored by observations of fluorescence indicators. 4. Elicitor-responsible genes were cloned by a differential display from plant tissues following treatment with elicitor, and characterized from a viewpoint of usage 5. Our devised experimental systems for determination of fundamental mechanism of induction of systemic OXB were improved to set as a manual for convenient screening of stimulator or activator of plant immunization. 6. Using the manual, we obtained active systemic OXB inducers with SAR inducing activity from several plants of 16 families and 26 species of tested plants

  58. ファイトアレキシン合成系遺伝子の構造と機能の解析

    Grant number:10760028  1998 - 1999

    科学研究費助成事業  奨励研究(A)

    吉岡 博文

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    Authorship:Principal investigator 

    Grant amount:\2000000 ( Direct Cost: \2000000 )

    本研究は、ジャガイモのファイトアレキシン合成の鍵酵素である3-Hydroxy-3-methylglutary1 CoA reductase (HMGR)遺伝子の推定プロモーター領域について検討したものである。
    病原菌接種およびエリシター処理により誘導されるHMG2およびHMG3遺伝子を単離して塩基配列を決定したところ、両ゲノムクローンとも3つのイントロンに分断される構造であった。これらの転写開始点を調べると、両者において翻訳開始コドンからおよそ60〜70塩基上流に複数の転写開始点が認められた。推定プロモーター領域の全長をルシフェラーゼ発現ベクターに組み込み、エレクトロポレーション法でジャガイモ塊茎プロトプラストに導入してプロモーター活性を調べた。HMG2の推定プロモーターでは、HWC処理区においてルシフェラーゼ活性の増加が認められた。デリーションクローンを構築してプロモーター活性を調べた結果、alternative oxidaseのプロモーター領域に見い出されたHFS boxおよびエリシター誘導性の防御遺伝子であるフェニルアラニンアンモニア・リアーゼのシスエレメントであるBox Aの関与が示唆された。
    同様にHMG3の推定プロモーター活性を調べた結果、エリシター誘導性の防御遺伝子であるカルコン合成酵素のプロモーター領域に見い出されたmyb配列およびフェニルアラニンアンモニア・リアーゼのシスエレメントであるBox Pの関与が浮かび上がってきた。また、デリーションの位置を考慮すると、約300bpの繰り返し配列の一ユニットではHWC誘電能を発揮し、この配列が繰り返されると、逆に抑制効果をもたらすことが考えられる。さらに、この繰り返し配列は、プロモーター活性を全体的に上昇させる機能を有するものと思われた。

  59. 植物のストレス応答における新規な全身的シグナル伝達系の解析

    Grant number:09876092  1997 - 1998

    科学研究費助成事業  萌芽的研究

    道家 紀志, 吉岡 博文

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    微生物の感染、異物の侵入、傷害刺激などを一部に受けた植物個体は、直接刺激を受けた組織のみならず全身的に、代謝変動と物質生産を速やかに起こす。刺激を受けた直後の組織では、その場で活性酸素生成反応(オキシダティブバースト:OXB)を急速に高めるが、この局部的なOXB誘導が全身的なOXBをも誘導する現象を見出した。これは、植物のストレス応答における新規な全身的シグナル伝達現象の存在を示唆した。本研究では、ジャガイモ塊茎組織およびその複葉ならびにタバコ植物の葉および個体を用い、局部的OXB誘導物質(菌体エリシター)の局部的刺激処理により、全身的OXBの発生を2種の活性酸素生成測定方法(ルミノールを介した化学発光測定および組織外シトクロムCのSOD感受性還元活性の測定)を開発・解析し、次の成果を得た。1.切断ジャガイモ組織表面へのエリシター処理で、切断後3時間以内は応答がないが、それ以後徐々にOXB応答が現れ、その二次元的発生の可視化・定量を可能にした。2.ジャガイモ塊茎の下端と上端を切断した加齢スライスで、下端断面へのエリシター処理で局部的OXBを誘導すると、数分のラグタイムを経て、上端切断面にOXBが発生した。3.加齢した縦切断塊茎スライスの側壁の一部をエリシター刺激すると、切断面上に、処理部付近から放射状に刻々とOXBが展開した。4.局部的OXBを担うO_2^-生成NADPH酸化酵素系を各種の阻害剤(Ca^<2+>キレート、Ca^<2+>チャネルブロッカ一など)およびカタラーゼやラジカルスカベンジャーの共存下の誘導刺激では、全身的OXBの誘導はなかった、過酸化水素が二次的刺激と判明した。5.全身的OXBの発生基盤は、O_2^-生成NADPH酸化酵素に依存するものと推察された。6.サリチル酸が全身シグナルと考えられているタバコ植物で、一部葉へのエリシター処理が、同一葉の中のみならず、葉柄を経て茎に、茎から他の葉に、サリチル酸関与以前の早さで、OXBを誘導した。7.これらの新現象から、局部的OXBと全身的OXBの発生をつなぐ全身的シグナルシステムの存在を提唱した。

  60. Molecular and physiological mechanism of oxidative burst in relation to signal transduction for defense response of plant to infection

    Grant number:09460027  1997 - 1998

    Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

    DOKE Noriyuki, YOSHIOKA Hirohumi, KAWAKITA Kazuhito

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    Plants cause induced defense response to pathogens or elicitor. Rapid activation of superoxide generating reaction (Oxidative burst : OXB) is induced at the beginning of induction of the defense response. This reaction system was shown to be dependent on an NADPH oxidase in plasma membrane regulated by signal trasnduction system involving Ca^<2+>, calumodulin, protein kinase, etc. In the present research, isolation and characterization of the NADPH oxidase, signal transduction stimulated by OXB and OXB-induced induction of defense-related genes were investigated with the following results. 1. Time course increase in potential for response with OXB on the sliced surface during aging after slicing of potato tuber. 2. Induction of sub-systemic OXB at aged sliced surface distant from elicitor treated side with local OXB.3. Kinetics of O_2 generating NADPH oxidase in isolated plasma membrane fraction of potato tuber. 4. Various inhibitor for Ca^<2+> signaling or protein kinase inhibited inductions of local and sub-systemic. 5. Solubirization of active 0_2 generating NADPH oxidase from plasma membrane by detergents. 6. Detection of several active bands in polyacril amid gel after electrophoresis of solubilized NADPH oxidase. 7. Cloning of cDNA homologous with gp91p^<phox> of human leukocytes and detection of homology with those from rice and Arabidopsis plants. 8. Detection of signal by antibody against p67^<phax> from human leukocytes in the fraction on SDS-PAGE of soluble protein from potato tuber tissues. 9. Inhibition by Ca^<2+> inhibitor of elicitor-stimulated OXB resulted in little induction of defense genes. lO.OXB stimulated phospholypase A_2 depending on a GTP binding protein. 11. OXB-related cytoplasmic aggregation was dependent on actin molecules with actin-binding proteins. 12. OXB did not necessarily associated with induction of enzymes related with phytoalexin production. 13. Further characterization of 0_2 generating NADPH oxidase was necessary for understanding OXB at molecular level of enzyme and gene.

  61. ジャガイモ塊茎組織におけるファイトアレキシン合成系酵素の活性化制御機構

    Grant number:05760044  1993

    科学研究費助成事業  奨励研究(A)

    吉岡 博文

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    Authorship:Principal investigator 

    Grant amount:\900000 ( Direct Cost: \900000 )

    ジャガイモ疫病では、宿主のジャガイモ品種と疫病菌のレースとの間で寄生性が厳密に分かれている。ジャガイモ塊茎組織では、ファイトアレキシンであるリシチンの蓄積が抵抗反応の主役を演じている。ジャガイモ塊茎に疫病菌の親和性・非親和性レースを接種し、リシチン合成の鍵酵素である3-ヒドロキシ-3-メチルグルタリルCoAリダクターゼ(HMGR)活性を経時的に調べたところ、本酵素活性は後者において顕著に増加した。全RNAを抽出してノーザン解析すると、構成的な蓄積に加え一過的なHMGR mRNAの蓄積増高がみられたが、両菌接種区において顕著な差は認められなかった。さらに、本酵素の活性化は新たなタンパク質の合成を伴うことを考え併せると、HMGRは転写以降から翻訳に至る過程で制御されるものと推定された。この可能性を探るために両菌を接種した塊茎組織から経時的に調製した粗酵素液中のHMGRタンパク質の量的変動を調べることを目的とし、HMGR cDNAよりタンパク質を合成して抗体の作製を試みた。しかしながら、十分な力価を有した抗体を得ることができなかった。そこで、本研究では本菌を接種した塊茎ディスクから経時的にポリソームRNAを調製し、HMGR mRNA量を調べた。その結果、ポリソームを形成したHMGR mRNA量の変動は酵素活性動向と相関し、そのレベルは非親和性菌接種区で顕著に高かった。さらに、12.5〜50%のショ糖密度勾配遠心により接種3時間後の塊茎ディスクからポリソームを分画してHMGR mRNAの分布を調べた。非親和性菌接種区に比べ、親和性菌接種区においてはラージポリゾーム画分でのmRNA量が少ないことが明らかとなった。接種6時間後になると、この傾向はさらに顕著になった。これらの結果より、HMGR活性の特異的経時変化はmRNAがポリソームを形成する過程(翻訳開始過程)で制御される可能性が伺われた。

  62. ジャガイモ塊茎組織におけるファイトアレキシン合成系遺伝子の発現制御

    1992.4 - 1993.3

    科学研究費助成事業  奨励研究(A)

    吉岡博文

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    Authorship:Principal investigator  Grant type:Competitive

    Grant amount:\900000 ( Direct Cost: \900000 )

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Industrial property rights 13

  1. Germ-Responsive Promoter

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    Application no:PCT/JP2003/015310  Date applied:2003.8

    Announcement no:WO 2004/050874 A1 

    Patent/Registration no:特許第4552021号  Date registered:2010.7 

    Country of applicant:Domestic  

  2. Germ-responsive promoter

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    Application no:03812352.7-2406  Date applied:2005.3

    Patent/Registration no:1574571  Date registered:2011.5 

    Country of applicant:Foreign country  

  3. 病原菌応答性プロモータ

    吉岡博文

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    Application no:200380109296.0  Date applied:2005.8

    Patent/Registration no:ZL 200380109296.0  Date registered:2008.10 

    Country of applicant:Foreign country  

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    Application no:10/537,094  Date applied:2006.3

    Announcement no:US-2006-005309-A1 

    Patent/Registration no:US 7,667,094 B2  Date registered:2010.2 

    Country of applicant:Foreign country  

  5. 害虫防除方法

    新美輝幸、吉岡博文、佐藤 豊

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    Application no:特願2009-136701  Date applied:2009.6

    Country of applicant:Domestic  

  6. 害虫防除方法

    新美輝幸、吉岡博文、佐藤 豊

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    Application no:PCT/JP2010/059499  Date applied:2010.6

    Country of applicant:Foreign country  

  7. MAPキナーゼバイオセンサー

    吉岡博文

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    Applicant:国立大学法人名古屋大学

    Application no:2017-038994  Date applied:2017.3

    Announcement no:2018-143119 

    Country of applicant:Domestic  

  8. 形質転換体

    吉岡博文・安達広明

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    Applicant:国立大学法人名古屋大学

    Application no:2020-021831  Date applied:2020.2

    Country of applicant:Domestic  

  9. 形質転換体

    吉岡博文, 安達広明

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    Applicant:国立大学法人名古屋大学

    Application no:2020-021831  Date applied:2020.2

  10. MAPキナーゼバイオセンサー

    吉岡博文

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    Applicant:国立大学法人名古屋大学

    Application no:2017-038994  Date applied:2017.3

    Announcement no:2018-143119 

  11. 害虫防除方法

    新美輝幸, 吉岡博文, 佐藤 豊

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    Application no:PCT/JP2010/059499  Date applied:2010.6

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    Application no:10/537,094  Date applied:2006.3

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    Patent/Registration no:US 7,667,094 B2  Date issued:2010.2

  13. Germ-Responsive Promoter

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    Application no:PCT/JP2003/015310  Date applied:2003.8

    Announcement no:WO 2004/050874 A1 

    Patent/Registration no:特許第4552021号  Date issued:2010.7

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Teaching Experience (On-campus) 24

  1. 生命農学序説

    2023

  2. Fundamentals of Biology II

    2023

  3. 専門セミナー

    2023

  4. Plant Pathology

    2023

  5. Plant Protection

    2023

  6. 卒業論文

    2023

  7. 資源生物科学基盤実験実習

    2023

  8. Advanced Plant Pathology

    2023

  9. 植物免疫学基盤実習

    2023

  10. 植物免疫学基盤講義

    2023

  11. 植物免疫学演習

    2023

  12. 修士論文研究

    2023

  13. 博士論文研究

    2023

  14. 生物学基礎II

    2020

  15. 専門セミナー

    2020

  16. 植物病理学

    2020

  17. 植物保護学

    2020

  18. 卒業論文

    2020

  19. 資源生物科学基盤実験実習

    2020

  20. 植物免疫学基盤実験

    2020

  21. 植物免疫学基礎講義

    2020

  22. 植物免疫学演習

    2020

  23. 植物病理学特論

    2020

  24. バイオダイナミクス輪講III

    2017

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Teaching Experience (Off-campus) 3

  1. 一般生物学

    1992.4 - 1993.3 金城学院大学短期大学部)

  2. 生物情報論

    2006.4 - 2007.3 岡山大学)

  3. 陸圏生物生産学特論

    2008.4 - 2009.3 三重大学)