Papers - NAKANO, Hideo
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Improvements in the cell-free production of functional antibodies using cell extract from protease-deficient Escherichia coli mutant. Reviewed
Muhamad Ali, Suzuki, H., Fukuba, T., Jiang, X., Nakano, H. and Yamane, T.
Journal of Bioscience and Bioengneering Vol. 99 page： 181-186. 2005
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Novel Strategy for Protein Exploration: High-throughput Screening Assisted with Fuzzy Neural Network, Reviewed
R. Kato, H. Nakano, H. Konishi, K. Kato, Y. Koga, T. Yamane, T. Kobayashi and H. Honda
Journal of Molecular Biology Vol. 35 ( 3 ) page： 683-692 2005
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*PCR amplification from single DNA molecules on magnetic beads in emulsion: application for high-throughput screening of transcription factor targets, Reviewed
T. Kojima, Y. Takei, M. Ohtsuka, Y. Kawarasaki, T. Yamane, and H. Nakano,
Nucleic Acids Research Vol. 33 page： e15 2005
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Mass enhancement cell-free protein synthesis using S30 extract from Escherichia coli growing fast at 42 degrees on amino acid-enriched medium. Reviewed
Yamane, T., Ikeda, Y., Nagasaka, T. and Nakano, H.
Biotechnology Progress Vol. 21 page： 608-613. 2005
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SIMPLEX法によるタンパク質ライブラリーの構築 Invited
科学と工業 Vol. 78 ( 6 ) page： 316-321 2004
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無細胞タンパク質合成系を用いたハイスループットスクリーニング技術の開発と応用
日本農芸化学会誌 Vol. 78 ( 5 ) page： 483-486 2004
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Cell-free protein synthesis systems: Increasing their performance and applications.
Nakano, H., Kawarasaki, Y., Yamane, T.
Advances in Biochemical Engineering/Biotechnology Vol. 90 page： 135-149 2004
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A picoliter chamber array for cell-free protein synthesis Reviewed
Takeshi Kinpara, Ryota Mizuno, Yuji Murakami, Masaki Kobayashi, Shouhei Yamamura, Quamrul Hasan, Yasutaka Morita, Hideo Nakano, Tsuneo Yamane, Eiichi Tamiya
Journal of Biochemistry Vol. 136 ( 2 ) page： 149-153 2004
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SIMPLEX法によるリパーゼのタンパク質工学
山根恒夫、中野秀雄
日本農芸化学会誌 Vol. 78 page： 751-753 2004
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Rapid screening for affinity-improved scFvs by means of single-molecule-PCR-linked in vitro expression. Reviewed
Rungpragayphan, S., Haba, M., Nakano, H. and Yamane, T.
Journal of Molecular Catalysis B: Enzymatic, Vol. 28 page： 223-228 2004
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*Inverting enantioselectivity of Burkholderia cepacia KWI-56 lipase by combinatorial mutation and high-throughput screening using single-molecule PCR and in vitro expression. Reviewed
Journal of Molecular Biology Vol. 331 ( 3 ) page： 585-592 2003.1
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Improvement of H 2O2stability of manganese peroxidase by combinatorial mutagenesis and high-throughput screening using in vitro expression with protein disulfide isomerase. Reviewed
Protein Engineering Vol. 16 ( 6 ) page： 423-428 2003
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PCR-linked in vitro expression: a novel system for high-throughput construction and screening of protein libraries. Reviewed
FEBS Lett Vol. 540 page： 147-150 2003
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Cloning and in vitro and in vivo expression of plant glutathione S-transferase zeta class gene. Reviewed
Journal of Bioscience and Bioengineering Vol. 95 ( 6 ) page： 594-600 2003
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Stabilization of Affinity-Tagged Recombinant Protein during/after Its Production in a Cell-Free System Using Wheat-Germ Extract. Reviewed
Journal of Bioscience and Bioengineering Vol. 95 ( 3 ) page： 209-214 2003
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*無細胞タンパク質合成系の高度化と応用 Invited
生物工学会誌 Vol. 81 ( 2 ) page： 71-76 2003
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High-throughput, cloning-independent protein library construction by combining single-molecule DNA amplification with in vitro expression. Reviewed
Journal of Molecular Biology Vol. 318 page： 395-405 2002
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In vitro combinatorial mutagenesis of the 65th and 22nd positions of the green fluorescent protein of Aequarea victoria. Reviewed
Biotechnology and Bioprocess Engineering Vol. 7 page： 1-6 2002
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Modifying the chain length selectivity of the lipase from Burkholderia cepacia KWI-56 through in vitro combinatorial mutagenesis in the substrate binding site. Reviewed
Protein Engineering Vol. 15 page： 147-152 2002
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Reduction of protein degradation by use of protease-deficient mutants in cell-free protein synthesis system of Escherichia coli. Reviewed
Journal of Bioscience and Bioengineering Vol. 93 ( 2 ) page： 151-156 2002