日本分子生物学会

日本農芸化学会   中部支部副支部長

Bio-nanocapsules for oriented immobilization of DNA aptamers on aptasensors Reviewed

Iijima Masumi, Yamada Yuki, Nakano Hideo, Nakayama Tsutomu, Kuroda Shun'ichi

ANALYST   Vol. 147 ( 3 ) page： 489 - 495   2022.1

無細胞タンパク質合成系を利用した迅速抗体スクリーニング技術開発と実用化 Invited

加藤晃代, 中野秀雄, 兒島孝明, 永井里美

日本生物工学会会誌   Vol. 99 ( 2 ) page： 62 - 67   2021.2

Development of robust and rapid monoclonal antibody screening technology using cell-free protein synthesis

Ojima-Kato T., Nakano H., Kojima T., Nagai S.

Seibutsu-kogaku Kaishi   Vol. 99 ( 2 ) page： 62 - 67   2021

Administration of Aspergillus oryzae suppresses DSS-induced colitis. International journal

Ryo Nomura, Sho Tsuzuki, Takaaki Kojima, Mao Nagasawa, Yusuke Sato, Masayoshi Uefune, Yasunori Baba, Toshiya Hayashi, Hideo Nakano, Masashi Kato, Motoyuki Shimizu

Food chemistry. Molecular sciences   Vol. 4   page： 100063 - 100063   2022.7

Structures of an engineered phospholipase D with specificity for secondary alcohol transphosphatidylation: Insights into plasticity of substrate binding and activation. Invited Reviewed International coauthorship

Samantha, A., Damnjanović, J., Iwasaki, Y., Nakano, H., and Vrielink, A.

Biochemical Journal   Vol. 478   page： 1749 - 1767   2021.5

Increasing cell-free gene expression yields from linear templates inEscherichia coliandVibrio natriegensextracts by using DNA-binding proteins Reviewed International coauthorship

Bo Zhu, Rui Gan, Maria D. Cabezas, Takaaki Kojima, Robert Nicol, Michael C. Jewett, Hideo Nakano

BIOTECHNOLOGY AND BIOENGINEERING   Vol. 117 ( 12 ) page： 3849 - 3857   2020.12

Development of a dual monoclonal antibody sandwich enzyme-linked immunosorbent assay for the detection of swine influenza virus using rabbit monoclonal antibody by Ecobody technology Reviewed

Daorung Sila-on, Phornnaphat Chertchinnapa, Yusuke Shinkai, Takaaki Kojima, Hideo Nakano

JOURNAL OF BIOSCIENCE AND BIOENGINEERING   Vol. 130 ( 2 ) page： 217 - 225   2020.8

A simple, real-time assay of horseradish peroxidase using biolayer interferometry

Takaaki Kojima, Ayako Nakane, Bo Zhu, Almasul Alfi, Hideo Nakano

Bioscience, Biotechnology, and Biochemistry   Vol. 83 ( 10 ) page： 1822 - 1828   2019.10

Production of active manganese peroxidase in Escherichia coli by co-expression of chaperones and in vitro maturation by ATP-dependent chaperone release

Almasul Alfi, Bo Zhu, Jasmina Damnjanović, Takaaki Kojima, Yugo Iwasaki, Hideo Nakano

Journal of Bioscience and Bioengineering   Vol. 128 ( 3 ) page： 290 - 295   2019.9

Comprehensive investigation of the gene expression system regulated by an Aspergillus oryzae transcription factor XlnR using integrated mining of gSELEX-Seq and microarray data. Reviewed

Oka H, Kojima T, Ihara K, Kobayashi T, Nakano H

BMC genomics   Vol. 20 ( 1 ) page： 16   2019.1

Acyl chain that matters: Introducing sn-2 acyl chain preference to a phospholipase D by protein engineering. Reviewed

Jasmina Damnjanović, Hideo Nakano, Yugo Iwasaki

Protein Eng. Des. Sel.   Vol. 32 ( 1 ) page： 1 - 11   2019.1

SKIK-zipbody-alkaline phosphatase, a novel antibody fusion protein expressed in Escherichia coli cytoplasm

Panwad Ritthisan, Teruyo Ojima-Kato, Jasmina Damnjanovic, Takaaki Kojima, Hideo Nakano

JOURNAL OF BIOSCIENCE AND BIOENGINEERING   Vol. 126 ( 6 ) page： 705 - 709   2018.12

Rapid Generation of Monoclonal Antibodies from Single B Cells by Ecobody Technology

Ojima-Kato Teruyo, Morishita Shiomi, Uchida Yoshino, Nagai Satomi, Kojima Takaaki, Nakano Hideo

ANTIBODIES   Vol. 7 ( 4 )   2018.11

Spatial arrangement of proteins using scCro-tag: application for an in situ enzymatic microbead assay Reviewed

Kojima Takaaki, Hata Jumpei, Oka Hiroya, Hayashi Kenta, Hitomi Kiyotaka, Nakano Hideo

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY   Vol. 82 ( 11 ) page： 1911 - 1921   2018.11

Zipbodyzyme: Development of new antibody-enzyme fusion proteins.

Mori A, Ojima-Kato T, Kojima T, Nakano H

Journal of bioscience and bioengineering     2018.2

Pilot-scale whole-cell biocatalysis for the hydroxylation of cyclosporine derivative, FR901459, at higher concentrations by Lentzea sp 7887 using soybean flour as a novel substrate dispersant

Yabutani Tetsuya, Shimizu Shiho, Nakano Hideo

JOURNAL OF BIOSCIENCE AND BIOENGINEERING   Vol. 123 ( 1 ) page： 56 - 62   2017.1

'Zipbody' leucine zipper-fused Fab in E-coli in vitro and in vivo expression systems

Ojima-Kato Teruyo, Fukui Kansuke, Yamamoto Hiroaki, Hashimura Dai, Miyake Shiro, Hirakawa Yuki, Yamasaki Tomomi, Kojima Takaaki, Nakano Hideo

PROTEIN ENGINEERING DESIGN & SELECTION   Vol. 29 ( 4 ) page： 149-157   2016.4

Handmade microfluidic device for biochemical applications in emulsion

Murzabaev Marsel, Kojima Takaaki, Mizoguchi Takuro, Kobayashi Isao, DeKosky Brandon J., Georgiou George, Nakano Hideo

JOURNAL OF BIOSCIENCE AND BIOENGINEERING   Vol. 121 ( 4 ) page： 471-476   2016.4

Directing positional specificity in enzymatic synthesis of bioactive 1-phosphatidylinositol by protein engineering of a phospholipase D Reviewed

Damnjanovic Jasmina, Kuroiwa Chisato, Tanaka Hidetoshi, Ishida Ken, Nakano Hideo, Iwasaki Yugo

BIOTECHNOLOGY AND BIOENGINEERING   Vol. 113 ( 1 ) page： 62-71   2016.1

Signal peptide optimization tool for the secretion of recombinant protein from Saccharomyces cerevisiae Reviewed

Mori Akihiro, Hara Shoichi, Sugahara Tomohiro, Kojima Takaaki, Iwasaki Yugo, Kawarasaki Yasuaki, Sahara Takehiko, Ohgiya Satoru, Nakano Hideo

JOURNAL OF BIOSCIENCE AND BIOENGINEERING   Vol. 120 ( 5 ) page： 518-525   2015.11

Role of disulfide bond isomerase DsbC, calcium ions, and hemin in cell-free protein synthesis of active manganese peroxidase isolated from Phanerochaete chrysosporium Reviewed

Ninomiya Ryoko, Zhu Bo, Kojima Takaaki, Iwasaki Yugo, Nakano Hideo

JOURNAL OF BIOSCIENCE AND BIOENGINEERING   Vol. 117 ( 5 ) page： 652-657   2014.5

Deletion of a Dynamic Surface Loop Improves Stability and Changes Kinetic Behavior of Phosphatidylinositol-Synthesizing Streptomyces Phospholipase D Reviewed

Damnjanovic Jasmina, Nakano Hideo, Iwasaki Yugo

BIOTECHNOLOGY AND BIOENGINEERING   Vol. 111 ( 4 ) page： 674-682   2014.4

A chromogenic substrate for solid-phase detection of phospholipase A(2) Reviewed

Eba Chisato, Okano Aoi, Nakano Hideo, Iwasaki Yugo

ANALYTICAL BIOCHEMISTRY   Vol. 447   page： 43-45   2014.2

Comprehensive Analysis of the DNA-Binding Specificity of an Aspergillus nidulans Transcription Factor, AmyR, by using a Bead Display System. Reviewed

Wang, P., Kojima, T., Kobayashi, T. and Nakano, H.

J. Biosci. Biotechnol. Biochem.   Vol. 76 ( 1 ) page： 1128-1134   2012.6

Comprehensive Analysis of the DNA-Binding Specificity of an Aspergillus nidulans Transcription Factor, AmyR, Using a Bead Display System

Wang Panhui, Kojima Takaaki, Kobayashi Tetsuo, Nakano Hideo

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY   Vol. 76 ( 6 ) page： 1128-1134   2012.6

Emulsion Culture: A Miniaturized Library Screening System Based on Micro-droplets in an Emulsified Medium. Reviewed

Kojima, T., Nagao, N., Ando, D., Ojima, T., Kawarasaki, Y., Kobayashi, I., Nakajima, M. and Nakano, H.

J. Biosci. Bioeng.   Vol. 112   page： 299-303   2011.6

GLOBE:Analysis of DNA-Protein Interaction Analysis. Invited

Kojima, T. and Nakano, H.

Methods in Molecular Biology,   Vol. 687   page： 307-317   2011

High-throughput Screening of DNA Binding Sites for Transcription Factor AmyR from Aspergillus nidulans Using DNA Beads Display System. Reviewed

Kojima, T., Hashimoto, Y., Kato, M., Kobayashi, T. and Nakano, H.

J. Biosci. Bioeng.   Vol. 109 ( 6 ) page： 519-525.   2010.6

High-throughput screening of DNA binding sites for transcription factor AmyR from Aspergillus nidulans using DNA beads display system

Kojima Takaaki, Hashimoto Yoko, Kato Masashi, Kobayashi Tetsuo, Nakano Hideo

JOURNAL OF BIOSCIENCE AND BIOENGINEERING   Vol. 109 ( 6 ) page： 519-525   2010.6

(2010) Directed Evolution of Angiotensin II-inhibiting Peptides Using Microbeads Display. Reviewed

Gan, R., Furuzawa, S., Kojima, T., Kanie, K., Kato, R., Okochi, M., Honda, H. and Nakano, H.

J. Biosci. Bioeng.   Vol. 109 ( 4 ) page： 411-417   2010.4

Synthesis of phosphatidylinositols having various inositol stereoisomers by engineered phospholipase D Reviewed

Akari Ozaki, Atsushi Masayama, Hideo Nakano, Yugo Iwasaki

J. Biosci. Bioeng.   Vol. 109   page： 337-340   2010.4

(2010) In vitro generation of anti-hepatitis B monoclonal antibodies from a single plasma cell using single-cell RT-PCR and cell-free protein synthesis. Reviewed

Sabrina, Y., Ali, M. and Nakano, H.

J. Biosci. Bioeng.   Vol. 109 ( 1 ) page： 75-82   2010.1

*Microbeads display of proteins using emulsion PCR and cell-free protein synthesis. Reviewed

Gan, R, Yamanaka, Y, Kojima, T, and Nakano, H.

Biotechnol. Prog.   Vol. 24   page： 1107-1114   2008.1

Streptomyces phospholipase D mutants with altered substrate specificity capable of phosphatidylinositol synthesis. Reviewed

Masayama, A., Takahashi, T., Tsukada, K., Nishikawa, S., Takahashi, R., Adachi, M., Koga, K., Suzuki, A., Yamane T., Nakano, H., and Iwasaki, Y.

Chembiochem   Vol. 9   page： 974-981   2008.1

Direct separation of monoacylglycerol isomers by enantioselective high-performance liquid chromatography. Reviewed

Deng, L., Nakano, H. and Iwasaki, Y.

J. Chromatogr. A.   Vol. 1198-1199   page： 67-72   2008.1

Direct separation of regioisomers and enantiomers of monoacylglycerols by tandem column high-performance liquid chromatography

Deng Li, Nakano Hideo, Iwasaki Yugo

JOURNAL OF CHROMATOGRAPHY A   Vol. 1165 ( 1-2 ) page： 93-99   2007.9

無細胞タンパク質合成系を用いた進化分子工学システムの構築と応用．

兒島孝明，中野秀雄

生化学   Vol. 79   page： 239 -246   2007.4

Development and application of evolutionary molecular engineering systems using cell-free protein synthesis

Kojima Takaaki, Nakano Hideo

SEIKAGAKU   Vol. 79 ( 3 ) page： 239-246   2007.3

Direct separation of regioisomers and enantiomers of monoacylglycerols by tandem column high-performance liquid chromatography. Reviewed

Deng, L., Nakano, H. and Iwasaki, Y.

J. Chromatogr.   Vol. 1198-1199   page： 93-99   2007.1

Creation of novel enantioselective lipases by SIMPLEX. Invited

Koga, Y., Yamane, T. and Nakano, H.

Methods Mol. Biol.   Vol. 375   page： 165-182   2007.1

SIMPLEX: single-molecule PCR-linked in vitro expression: a novel method for high-throughput construction and screening of protein libraries. Invited

Rungpragayphan, S., Yamane, T. and Nakano, H.

Methods Mol. Biol.   Vol. 375   page： 79-94   2007.1

High-speed and high sensitive immunochemical detection using pillar arrays Reviewed

  Vol. 126 ( 8 ) page： 486-491   2006.8

In vitro selection of DNA binding sites for transcription factor, PhaR, from Paracoccus denitrificans using genetic library on microbeads and flow cytometry

Kojima Takaaki, Yamane Tsuneo, Nakano Hideo

JOURNAL OF BIOSCIENCE AND BIOENGINEERING   Vol. 101 ( 5 ) page： 440-444   2006.5

Generation of Monoclonal Antibodies Using Simplified Single-Cell Reverse Transcription-Polymerase Chain Reaction and Cell-Free Protein Synthesis. Reviewed

Muhamad Ali, Hitomi, K. and Nakano, H.

Journal of Bioscience and Bioengineering,   Vol. 101,   page： 284-286   2006.4

*A Novel Strategy for Generation of Monoclonal Antibodies from Single B Cells Using RT-PCR Technique and in Vitro Expression. Reviewed

XiuPing Jiang, Suzuki, H., Hanai, Y., Wada, F., Hitomi, K., Yamane, T. and Nakano, H.

Biotechnol. Prog.   Vol. 22   page： 979-988   2006.4

In vitro selection of DNA binding sites for transcription factor, PhaR, from Paracoccus denitrificans using genetic library on microbeads and flow cytometry. Reviewed

Kojima, T., Yamane, T. and Nakano, H.

Journal of Bioscience and Bioengineering   Vol. 101   page： 440-444.   2006.3

Improvements in the cell-free production of functional antibodies using cell extract from protease-deficient Escherichia coli mutant. Reviewed

Muhamad Ali, Suzuki, H., Fukuba, T., Jiang, X., Nakano, H. and Yamane, T.

Journal of Bioscience and Bioengneering   Vol. 99   page： 181-186.   2005

Novel Strategy for Protein Exploration: High-throughput Screening Assisted with Fuzzy Neural Network, Reviewed

R. Kato, H. Nakano, H. Konishi, K. Kato, Y. Koga, T. Yamane, T. Kobayashi and H. Honda

Journal of Molecular Biology   Vol. 35 ( 3 ) page： 683-692   2005

*PCR amplification from single DNA molecules on magnetic beads in emulsion: application for high-throughput screening of transcription factor targets, Reviewed

T. Kojima, Y. Takei, M. Ohtsuka, Y. Kawarasaki, T. Yamane, and H. Nakano,

Nucleic Acids Research   Vol. 33   page： e15   2005

Mass enhancement cell-free protein synthesis using S30 extract from Escherichia coli growing fast at 42 degrees on amino acid-enriched medium. Reviewed

Yamane, T., Ikeda, Y., Nagasaka, T. and Nakano, H.

Biotechnology Progress   Vol. 21   page： 608-613.   2005

無細胞タンパク質合成系を用いたハイスループットスクリーニング技術の開発と応用

日本農芸化学会誌   Vol. 78 ( 5 ) page： 483-486   2004

A picoliter chamber array for cell-free protein synthesis Reviewed

Takeshi Kinpara, Ryota Mizuno, Yuji Murakami, Masaki Kobayashi, Shouhei Yamamura, Quamrul Hasan, Yasutaka Morita, Hideo Nakano, Tsuneo Yamane, Eiichi Tamiya

Journal of Biochemistry   Vol. 136 ( 2 ) page： 149-153   2004

SIMPLEX法によるリパーゼのタンパク質工学

山根恒夫、中野秀雄

日本農芸化学会誌   Vol. 78   page： 751-753   2004

Rapid screening for affinity-improved scFvs by means of single-molecule-PCR-linked in vitro expression. Reviewed

Rungpragayphan, S., Haba, M., Nakano, H. and Yamane, T.

Journal of Molecular Catalysis B: Enzymatic,   Vol. 28   page： 223-228   2004

Cell-free protein synthesis systems: Increasing their performance and applications.

Nakano, H., Kawarasaki, Y., Yamane, T.

Advances in Biochemical Engineering/Biotechnology   Vol. 90   page： 135-149   2004

SIMPLEX法によるタンパク質ライブラリーの構築 Invited

科学と工業   Vol. 78 ( 6 ) page： 316-321   2004

*Inverting enantioselectivity of Burkholderia cepacia KWI-56 lipase by combinatorial mutation and high-throughput screening using single-molecule PCR and in vitro expression. Reviewed

Journal of Molecular Biology   Vol. 331 ( 3 ) page： 585-592   2003.1

Improvement of H 2O2stability of manganese peroxidase by combinatorial mutagenesis and high-throughput screening using in vitro expression with protein disulfide isomerase. Reviewed

Protein Engineering   Vol. 16 ( 6 ) page： 423-428   2003

PCR-linked in vitro expression: a novel system for high-throughput construction and screening of protein libraries. Reviewed

FEBS Lett   Vol. 540   page： 147-150   2003

Cloning and in vitro and in vivo expression of plant glutathione S-transferase zeta class gene. Reviewed

Journal of Bioscience and Bioengineering   Vol. 95 ( 6 ) page： 594-600   2003

Stabilization of Affinity-Tagged Recombinant Protein during/after Its Production in a Cell-Free System Using Wheat-Germ Extract. Reviewed

Journal of Bioscience and Bioengineering   Vol. 95 ( 3 ) page： 209-214   2003

*無細胞タンパク質合成系の高度化と応用 Invited

生物工学会誌   Vol. 81 ( 2 ) page： 71-76   2003

High-throughput, cloning-independent protein library construction by combining single-molecule DNA amplification with in vitro expression. Reviewed

Journal of Molecular Biology   Vol. 318   page： 395-405   2002

In vitro combinatorial mutagenesis of the 65th and 22nd positions of the green fluorescent protein of Aequarea victoria. Reviewed

Biotechnology and Bioprocess Engineering   Vol. 7   page： 1-6   2002

Modifying the chain length selectivity of the lipase from Burkholderia cepacia KWI-56 through in vitro combinatorial mutagenesis in the substrate binding site. Reviewed

Protein Engineering   Vol. 15   page： 147-152   2002

Reduction of protein degradation by use of protease-deficient mutants in cell-free protein synthesis system of Escherichia coli. Reviewed

Journal of Bioscience and Bioengineering   Vol. 93 ( 2 ) page： 151-156   2002

In vitro construction and screening of Burkholderia cepacia lipase library using single-molecule PCR and cell-free protein synthesis. Reviewed

Journal of Bioscience and Bioengineering   Vol. 94 ( 1 ) page： 84-86   2002

Expression of Fab fragment of catalytic antibody 6D9 in an Escherichia coli in vitro coupled transcription/translation system. Reviewed

FEBS Letters,   Vol. 514   page： 290-294   2002

無細胞蛋白質合成系による新機能分子の創出

化学と生物   Vol. 40 ( 8 ) page： 533-538   2002

無細胞タンパク質合成系の進歩と応用

現代化学   Vol. 370   page： 66-72   2002

無細胞系によるタンパク質創製システム

バイオインダストリー   Vol. 18 ( 6 ) page： 42-48   2001

PhaR, a protein of unknown function conserved among short-chain-length polyhydroxyalkanoic acids producing bacteria, is a DNA-binding protein and represses Paracoccus denitrificans phaP expression in vitro. Reviewed

FEMS Microbiol Lett.   Vol. 200 ( 1 ) page： 9-15   2001

Dosage effect of minor arginyl- and isoleucyl-tRNA on protein synthesis in an E.coli in vitro coupled transcription/translation system. Reviewed

Journal of Bioscience and Bioengineering   Vol. 91 ( １ ) page： 53-57   2001

"Good-bye“大腸菌"":無細胞タンパク質合成系の挑戦"

中野秀雄

バイオサイエンスとインダストリー   Vol. 58   page： 11-15   2000.1

A trimmed virul cap-independent translation enhancing sequence for rapid in vitro gene expression.

"Kawarasaki,Y. Kasahara,S., Kodera,N., Shinbata,T., Sekiguchi,S., Nakano, H.,and Yamane"

Biotechnology Progress   Vol. 16   page： 517-521   2000.1

Importance of disulfide bridge formation on folding of phospholipase D from Streptomyces antibioticus. Reviewed

Journal of Bioscience and Bioengineering   Vol. 89 ( 5 ) page： 506-508   2000

Single-step Single-Molecule PCR of DNA with a homo-priming sequence using a single primer and hot-startable DNA polymerase Reviewed

Journal of Bioscience and Bioengineering   Vol. 90   page： 456   2000

In vitro analysis of roles of a disulfied bridge and a calcium binding site in activation of Pseudomonas sp. strain KWI-56 lipase. Reviewed

Journal of Bacteriology   Vol. 182   page： 295-302   2000

Amalyses of a polybydroxy alkanoic acid granule-associated 16-kilodalton protein and its patatire regulator in the pha locus of Paracoccus denitrificans. Reviewed

Journal of Bacteriology   Vol. 121 ( 9 ) page： 2914-2921   1999

Efficient coupled transcrption /trornslation for PCR template by a hollow fiber membra in bioreactar. Reviewed

Biotechnology and Bioengineering   Vol. 64 ( 2 ) page： 194-199   1999

Modulator-mediated synthesis of active lipase of Pseudomonas sp. 109 by Escherichia coli cell-free coupled transcription/translation system. Reviewed

Journal of Bioscience and Bioengineering   Vol. 88   page： 605-609   1999

Purification, characterization and gene cloning of 6-hydroxy nicotinate 3-monooyygenase from Pseadomonas flaovescene 7N5. Reviewed

European Journal of Biachemistry   Vol. 260   page： 120-126   1999

無細胞たん白質合成

山根恒夫、中野秀雄、河原崎泰昌

ケミカル・エンジニアリング   Vol. 43   page： 198-206   1998.1

Two distinct phosphatidylinositol-specific phospholipase Cs from Streptomyces antibioticus.

"Iwasaki, Y., Tsubouchi, Y., Ichihashi, A., Nakano, H., Kobayashi, T., Ikezawa, H., and Yamane, T.,"

Biochemica Biophysica Acta   Vol. 1391   page： 52-66   1998.1

In vitro method for the generation of protein libraries using PCR amplification of a single DNA molecule and coupled transciptin /translation. Reviewed

Nucleic Acids Research   Vol. 26 ( 19 ) page： 4339-4346   1998

Cell-free protein synthesis systems.

Biotechnolosy Advances   Vol. 16 ( 2 ) page： 367-384   1998

Phosphatase-immunodepleted cell-free protein synthesis system. Reviewed

Journal of Biotechnology   Vol. 61   page： 199-202   1998

Insertion of stabilizing loci in vectors of T7 RNA polymerase-mediated scherichia coliexpression systems: A case study on the plasmids involving foreign phospholipase D gene

"Mishima, N., Mizumoto, K., Iwasaki, Y., Nakano, H. and Yamane, T.,"

Biotechnology Progress   Vol. 13   page： 864-868   1997.1

Accumulation of translational inhibitors during multi-hour cell-free protein synthesis reaction using rabbit reticalocy of lysate. Reviewed

Journal of Fermentation and Bioengineering   Vol. 83 ( 5 ) page： 470-473   1997

Recent advances in cell-free protein synthesis towards a protein biosynthesizer Invited Reviewed

"Nakano, H., Kawarasaki, Y., and Yamane, T."

Enzyme Engineering XIII (The Annals of the New York Academy of Sciences)   Vol. 799   page： 406-412   1996.1

Highly productive cell-free protein synthesis system using coensed wheat-germ extract

"Nakano, H., Tanaka, T., Kawarasaki, Y. and Yamane, T.,"

Journal of Biotechnology   Vol. 46   page： 275-282   1996.1

Highly productive cell-free protein synthesis system using condensed wheat-germ extract. Reviewed

Journal of Biotechnolosy   Vol. 46   page： 275-282   1996

Purification and some properties of serm acid phosphatases. Reviewed

Plant Science   Vol. 119   page： 67-77   1996

無細胞系での蛋白質大量合成に向けて. ―─ 遺伝子翻訳機の構築 ――

中野秀雄、山根恒夫

バイオサイエンスとインダストリー   Vol. 53   page： 967-969   1995.1

A long-lived batch reaction system of cell-free protein synthesis

"Kawarasaki, Y., Kawai, T., Nakano, H. and Yamane, T.,"

Analytical Biochemistry   Vol. 226   page： 320-324   1995.1

n VitroProtein Biosynthesis Using Ribosome and Foreign mRNA - An Approach to Construct a Protein Biosynthesizer -

"Yamane, T., Kawarasaki, Y. and Nakano, H."

Enzyme Engineering XII (The Annals of the New York Academy of Sciences)   Vol. 750   page： 146-157   1995.1

A long-lived batch reaction system of cell free protein synthsis. Reviewed

Analytical Biochemistry   Vol. 226 ( 2 ) page： 320-324   1995

In Vitro Protein Biosynthesis Using Ribosome and Foreign mRNA An Approach to Construct a Protein Biosynthesizer. Reviewed

Enzyme Engineering XII   Vol. 750   page： 146-157   1995

Extracellular production of phospholipase D of Streptmyces antibioticus by recombinant Escherichia coli. Reviewed

Journal of Fermentation and Bioengineering   Vol. 79 ( 5 ) page： 417-421   1995

High speed polymerase chain reaction in Constant flow. Reviewed

Bioscience, Biotechnology and Biochemistry   Vol. 58 ( 2 ) page： 349-352   1994

An increased rate of cell-free protein synthesis by condenleing wheat-gem extuet with ultrafiltration menbranes. Reviewed

Bioscience, Biotechnology and Biochemistry   Vol. 58 ( 4 ) page： 631-634   1994

Role of cystein residues in esterose from Bacillus stearothermophilus and increasing its thermostability by the replacement of cysteine. Reviewed

Applied Microbiology and Biotechnology   Vol. 40 ( 3 ) page： 664-668   1994

PCRの高速化

中野秀雄、山根恒夫

蛋白質　核酸　酵素   Vol. 39   page： 2113-2120   1994

Secretion of a Cytoplasmic Protein Using Escherichia coli α-Hemolysin Membrane Transport System Reviewed

  Vol. 17   page： 3   1991

シグナル配列によらないタンパク質の膜透過系

ＢＩＯｍｅｄiｃａ   Vol. 6   page： 186-190   1991

SIMPLEX法の開発と進化分子工学への応用.　コンビナトリアル・バイオエンジニアリングの最前線　監修植田充美

今村千絵、中野秀雄（ Role： Joint author）

シーエムシー出版  2004 

In vitro expression of proteins with disulfide bridges and its application for high-throughput screening system. Cell-free protein expression ed. James R. Swartz

Hideo Nakano and Tsuneo Yamane（ Role： Joint author）

Springer  2003 

マイクロリアクタアレイによる超高密度タンパク質・ペプチド分子ライブラリの構築

中野 秀雄（ Role： Joint author）

[出版者不明]  2003 

無細胞タンパク質合成系による蛋白質工学. 化学フロンティア「コンビナトリアル・バイオエンジニアリング」（ 編：植田充美,近藤昭彦）"

中野秀雄,古賀雄一（ Role： Joint author）

化学同人  2002 

無細胞タンパク質合成系の最近の進歩 化学フロンティア『コンビナトリアル・バイオエンジニアリング』植田、近藤編

中野秀雄（ Role： Joint author）

化学同人  2002 

日本生化学会編基礎生化学実験法

中野秀雄（ 編：）（ Role： Joint author）

東京化学同人  2001 

高効率小麦胚芽タンパク質生合成系，

遺伝子発現研究法（学会出版センター）  2000 

無細胞分子進化システムの構築

中野 秀雄（ Role： Joint author）

[出版者不明]  1999 

シングルB細胞由来モノクローナル抗体の迅速取得と大腸菌による生産

加藤晃代, 兒島孝明, 中野秀雄

日本農芸化学会大会講演要旨集(Web)   Vol. 2018   2018

シングルB細胞由来モノクローナル抗体の迅速取得と大腸菌による生産

加藤晃代, 兒島孝明, 田村廣人, 中野秀雄

化学工学会秋季大会研究発表講演要旨集(CD-ROM)   Vol. 49th   2017

モノクローナル抗体取得技術の新展開 より良い抗体をより早く

加藤晃代, 加藤晃代, 中野秀雄

化学と生物   Vol. 55 ( 7 )   2017

大腸菌発現システムを用いたシングルヒトB細胞からの抗乳酸菌ヒトモノクローナル抗体取得

内田由乃, 加藤晃代, 兒島孝明, 中野秀雄

日本農芸化学会大会講演要旨集(Web)   Vol. 2017   2017

マイクロチャネル(MC)乳化法を用いたエマルジョンPCRによるマイクロビーズへのDNAライブラリーの構築．

杉浦一輝，小林　功，中嶋光敏，中野秀雄：

日本農芸化学会2007年度大会 

Altering substrate specificity of phospholipase D by directed evolution. International conference

The Japan-Italy Symposium of New Trends in Enzyme Science and Technology, 

Protein engineering of lipase using cell-free protein synthesis system and information technology. International conference

Japan-Italy Symposium of New Trends in Enzyme Science and Technology, 

Direct separation of regio-and enantiomeric isomers of monoglycerides by a tandem column HPLC.

Deng Li, Iwasaki, Y., and Nakano, H.:

マイクロビーズ上での分子ディスプレイ法を用いたDNA-転写因子複合体検出法．

橋本陽子，兒島孝明，加藤雅士，小林哲夫，中野秀雄

日本農芸化学会中部支部例会 

糸状菌β―ガラクトシダーゼをレポーターとする半定量的酵母２ハイブリッド系の開発

小島晃代，神谷拓摩，池内暁紀，中野秀雄，河原崎泰昌

日本農芸化学会中部支部例会 

Exploring novel enantio-selective lipases by high-throughput screening with the aid of fuzzy neural network analysis International conference

The Ninth Japan-China-Korea Joint Symposium on Enzyme Engineering 

Altering substrate specificity of phospholipase D by directed evolution. International conference

A Novel Compact Disk-like Microfluidic Device with Micropillars for Enzyme-linked Immunosorbent Assay.

無細胞蛋白質合成系を用いたリパーゼの蛋白質工学

日本生物工学会平成18年度大会， 

位置特異的アミノ酸飽和変異による2-デオキシリボース-5-リン酸アルドラーゼの基質特異性改変．

木村昌博，則武智哉，河原崎泰昌，岩崎雄吾，吉田洋一，中野秀雄

日本生物工学会平成18年度大会 

特定蛋白質間相互作用の選択的破壊法の開発．

池内暁紀，神谷拓摩，山根恒夫，中野秀雄，河原崎泰昌

日本生物工学会平成18年度大会 

フローサイトメトリーを用いた転写因子AmyR-DNA複合体の検出法。

橋本陽子、兒島孝明、加藤雅士、小林哲夫、中野秀雄

日本農芸化学会2006年度大会 

無細胞タンパク質合成系の高度化と応用

中野秀雄

日本農芸化学会2006年度大会 

ホスファチジルイノシトール合成活性を有する変異型ホスホリパーゼDの創製。

高橋哲也、山根恒夫、中野秀雄、岩崎雄吾

日本農芸化学会2006年度大会 

エマルジョンPCRを用いたマイクロビーズ上での分子ディスプレイ法の開発

山中友美子、兒島孝明、山根恒夫、中野秀雄

日本農芸化学会2006年度大会 

Protein-protein interaction network in Yeast kinetochore.

糸状菌β－ガラクトシダーゼを相互作用レポーターとする半定量的酵母２ハイブリッド系の開発。

小島晃代、河原崎泰昌、神谷拓摩、池内暁紀、中野秀雄

日本農芸化学会2006年度大会 

:Exhaustive identification of interaction domains using a novel high-throughput method: The first step toward interaction targeting. International conference

PACIFICHEM 2005 

Novel techniques using PCR and cell-free protein synthesis systems for combinatorial bioengineering. International conference

PACIFICHEM 2005 

PCR amplification from single DNA molecule on magnetic bead in emulsion: Application for high-throughput screening of transcription factor targets. International conference

PACIFICHEM 2005 

Enzymatic preparation of enantiomerically pure2,3-diacylglycerol.

構造脂質の酵素合成

岩崎雄吾、ピヤティラウォンウィーラ、中野秀雄、山根恒夫：

日本生物工学会平成17年度大会 

進化工学的手法を用いた出芽酵母キネトコアDam1複合体のサブユニット間相互作用領域の同定

神谷拓摩、池内暁紀、河原崎泰昌、中野秀雄、山根恒夫

日本生物工学会平成17年度大会 

Generation and Screening of Monoclonal Antibodies Using Single-Step Single Cell RT-PCR linked in vitro Expression

Muhamad Ali, Hitomi, K., and Nakano, H.

日本生物工学会平成17年度大会 

W/Oエマルジョン内での固相1分子PCR無細胞蛋白質合成系を用いた蛋白質間相互作用検出法の開発。

山中友美子、兒島孝明、山根恒夫、中野秀雄

日本生物工学会平成17年度大会 

マイクロビーズ上での遺伝子ライブラリーを用いたDNA 結合タンパク質の結合DNA領域のハイスループットスクリーニング法の開発。

兒島孝明、武井義明、山根恒夫、中野秀雄

日本生物工学会平成17年度大会 

位置特異的アミノ酸飽和変異による2-デオキシリボース-5-リン酸アルドラーゼの基質特異性改変(2)。

木村昌博、則武智哉、河原崎泰昌、岩崎雄吾、吉田洋一、中野秀雄

日本生物工学会平成17年度大会 

: Generation and Screening of Monoclonal Antibodies Using Single-Step Single Cell RT-PCR linked in vitro Expression.

A novel approach for generation and screening of monoclonal antibody by single cell RT-PCR and in vitro expression.

W/Oエマルジョン内１分子固相PCR法の開発。

武井義明、兒島孝明、民谷栄一、山根恒夫、中野秀雄

日本農芸化学会2005年度大会 

ビーズディスプレイ法によるDNA結合タンパク質の結合DNA領域のスクリーニング法の開発。

兒島孝明、武井義明、山根恒夫、中野秀雄

日本農芸化学会2005年度大会 

有機鎖修飾メソポーラスシリカに固定化されたリパーゼの活性安定性。

森岡幸、加藤且也、斎藤隆雄、Sindhu Seelan、横川善之、高橋治雄、中野秀雄、山根恒夫

第８回生体触媒化学シンポジウム 

中野秀雄、山根恒夫：SIMPLEX法による新規蛋白質の創製。

第27回日本分子生物学会年会 

Exploring novel enantio-selective lipases by high-throughput screening with the aid of fuzzy neural network analysis. International conference

The 10th APCChe congress 

SIMPLEXを用いた新規単鎖抗体ライブラリーの構築とスクリーニング

山中友美子、Rungpragayphan Suang、円谷　健、藤井郁雄、中野秀雄、山根恒夫

日本生物工学会平成16年度大会 

SIMPLEX法により得られた光学選択性の反転した変異リパーゼ群の解析

加賀哲也、中野秀雄、小西宏幸、古賀雄一、加藤克也、山根恒夫

日本生物工学会平成16年度大会 

Creation of novel proteins by SIMPLEX. International conference

3rd Japan-Korea Workshop on Molecular Display, 

Development of orthgonal liposomal fusion method using artificial phospholipids and its application

Grant number：19K05160  2019.4 - 2022.3

Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

Simultaneous production of monoclonal antibodies against multiple membrane proteins and their evaluations for the applications to therapeutic medicines

Grant number：17H03468  2017.4 - 2021.3

Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

Tomita Masahiro

近赤外光を用いた新規迅速免疫測定法の開発

2014.4 - 2016.3

科学研究費補助金  挑戦的萌芽研究、課題番号：26630425

中野　秀雄

無細胞系ナノ構造化生物機能システムの開発

2014.4 - 2016.3

科学研究費補助金  特定領域研究

中野　秀雄

無細胞系ギガスクリーニング法の開発と応用

2011.4 - 2015.3

科学研究費補助金  基盤研究(B)

中野　秀雄

汎用的シグナルペプチド配列最適化法の開発

2011.4 - 2013.3

科学研究費補助金  挑戦的萌芽研究、課題番号：23656522

中野　秀雄

免疫細胞ネットワークのデジタル解析

2007.4 - 2010.3

科学研究費補助金  特定領域研究（公募，A03），課題番号：19021020

中野　秀雄

無細胞系分子ディスプレイシステムの構築と応用

2007.3 - 2011.3

科学研究費補助金  基盤研究（Ｂ）（一般），課題番号：19360373

中野　秀雄

ヒトモノクローナル抗体のハイスループット取得法の開発

2007.3 - 2009.3

科学研究費補助金  萌芽研究，課題番号：19656218

中野　秀雄

酵素の光学選択性に関与する基質認識部位の網羅的解析

2004.7 - 2007.3

科学研究費補助金  基盤研究（Ｂ），課題番号：16360411

中野秀雄

細胞１個の遺伝情報とリンクしたプロテインアレイシステムの構築

2004.7 - 2005.3

科学研究費補助金  萌芽研究，課題番号：16656256

中野秀雄

マイクロリアクタアレイによる超高密度タンパク質・ペプチド分子ライブラリの構築

2000.7 - 2002.1

科学研究費補助金  基盤研究(B)(1)(一般)

ハイスループットモノクローナル抗体取得法に関する研究

2000.7 - 2001.1

科学研究費補助金  萌芽的研究

バイオ研究支援機器としての無細胞遺伝子翻訳装置の開発

1998.7 - 2002.1

科学研究費補助金  基盤研究(B)(2)(展開)

無細胞分子進化システムの構築

1997.7 - 1999.1

科学研究費補助金  基盤研究(C)(2)(一般)

無細胞蛋白質合成系の効率化に関する研究

1995.1 - 1997.12

科学研究費補助金  一般研究(B)

リガンド親和性複合蛋白質の取得方法、抗体スクリーニング方法、抗体スクリーニング用キット、医療方法、抗体医薬、非免疫反応性抗体医薬、投薬方法

中野秀雄　姜　秀ピン

タンパク質の生産方法、タンパク質のスクリーニング方法、及びタンパク質の機能検索方法

今村 千絵,高橋 治雄,中野 秀雄,山根 恒夫

Method for amplifying nucleic acid molecules and method for synthesizing proteins,"

タンパク質の合成方法

山根 恒夫, 中野 秀雄,河原崎 泰昌,関口 哲

タンパク質の合成方法

関口 哲,新畑 智也,布藤 聡,中野 秀雄,山根 恒夫

核酸分子の増幅方法

山根 恒夫,中野 秀雄,関口 哲

核酸分子の増幅方法及びタンパクの合成方法

山根 恒夫, 中野 秀雄,大内 将司, 奥村 れい子, 関口 哲

タンパクの製造方法

山根 恒夫, 中野 秀雄, 奥村 れい子,

無細胞タンパク合成系によるタンパクの合成方法及び装置

山根恒夫、中野秀雄、田中忠明、関口哲

６－ヒドロキシニコチン酸モノオキシゲナーゼ遺伝子

中野 秀雄, 川合 隆博,山根 恒夫, 長澤 透

無細胞タンパク合成系を用いたタンパクの製造法

関口 哲,船山 綾子,久保田 肇,山根 恒夫,中野 秀雄

無細胞タンパク合成系を用いたタンパクの製造法

山根恒夫、中野秀雄

ＤＮＡ増幅方法及びＤＮＡ増幅装置

中野 秀雄, 山根 恒夫,長棟 輝行,養王田 正文遠藤 勲

First Year Seminar B

2011