Updated on 2023/05/01

写真a

 
NAKANO, Hideo
 
Organization
Graduate School of Bioagricultural Sciences Department of Applied Biosciences Professor
Institute of Liberal Arts and Sciences Headquarters Part-time faculty member
Graduate School
Graduate School of Bioagricultural Sciences
Undergraduate School
School of Agricultural Sciences
Title
Professor
Contact information
メールアドレス

Degree 2

  1. 工学博士 ( 1992.12   東京大学 ) 

  2. 工学修士 ( 東京大学 ) 

Research Interests 7

  1. Antibody engineering

  2. Cell-free protein synthesis

  3. Protein engineering

  4. enzyme

  5. Cell-free protein synthesis

  6. antibody

  7. High throughput screening

Research Areas 2

  1. Others / Others  / Bioengineering

  2. Manufacturing Technology (Mechanical Engineering, Electrical and Electronic Engineering, Chemical Engineering) / Biofunction and bioprocess engineering

Current Research Project and SDGs 5

  1. Studies on cell-free protein synthesis

  2. 無細胞蛋白質合成系を用いたヒトB細胞からのモノクローナル抗体合成法の開発と応用

  3. Creation of novel protein using cell-free protein synthesis system

  4. Protein Engineering

  5. Development of nano-structured protein arrangement

Research History 4

  1. Nagoya University   Trustee / Vice President   Presidential Advisor

    2016.8 - 2618.3

  2. 名古屋大学大学院生命農学研究科・教授

    2005.4

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    Country:Japan

  3. 名古屋大学農学部 助教授

    1995.5 - 2005.3

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    Country:Japan

  4. 名古屋大学農学部 助手

    1991.1 - 1995.12

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    Country:Japan

Education 2

  1. The University of Tokyo   Graduate School, Division of Engineering

    - 1991

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    Country: Japan

  2. The University of Tokyo   Faculty of Engineering

    - 1985

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    Country: Japan

Professional Memberships 7

  1. 日本農芸化学会   理事

    2021.5

  2. 日本生物工学会   理事

    2015.5 - 2021.4

  3. 日本農芸化学会   中部支部庶務幹事

    2000.4 - 2003.3

  4. 日本化学工学会   中部支部庶務幹事

    1996.4 - 1999.3

  5. 日本農芸化学会   中部支部庶務幹事

    1996.4 - 1999.3

  6. 日本分子生物学会

  7. 日本農芸化学会   中部支部副支部長

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Awards 5

  1. 生物工学技術賞

    2021.9   公益社団法人日本生物工学会   無細胞タンパク質合成系を利用した迅速抗体スクリーニング技術開発とその実用化

    加藤晃代、中野秀雄、兒島孝明、永井 里美

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    Award type:Award from Japanese society, conference, symposium, etc.  Country:Japan

    無細胞タンパク質合成系を利用した新規な迅速抗体スクリーニング技術開発を実用化し、大学発ベンチャー企業設立に導いた。

  2. 生物工学論文賞

    2011.9   公益社団法人日本生物工学会  

    兒島孝明 、橋本陽子、加藤雅士、小林哲夫、中野秀雄

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    Award type:Honored in official journal of a scientific society, scientific journal  Country:Japan

  3. 日本生物工学会 論文賞

    2004.9   日本生物工学会  

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    Country:Japan

  4. 2002年武田研究奨励賞優秀賞

    2002.11   (財)武田計測先端知財団  

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    Country:Japan

  5. "Terui Award, The Society for Biotechnology, Japan"

    2002.10  

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    Country:Japan

 

Papers 110

  1. Leucine Zipper fused Fab; Enhancement of active Fab formation in E. coli in vitro and in vivo expression systems Reviewed

    Ojima-Kato Teruyo, Fukui Kansuke, Kojima Takaaki, Nakano Hideo

    PROTEIN SCIENCE   Vol. 24   page: 194-194   2015.10

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  2. Ultra-High-Throughput Screening of an In Vitro-Synthesized Horseradish Peroxidase Displayed on Microbeads Using Cell Sorter Reviewed

    Zhu Bo, Mizoguchi Takuro, Kojima Takaaki, Nakano Hideo

    PLOS ONE   Vol. 10 ( 5 )   2015.5

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    DOI: 10.1371/journal.pone.0127479

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  3. Immobilization of proteins onto microbeads using a DNA binding tag for enzymatic assays Reviewed

    Kojima Takaaki, Mizoguchi Takuro, Ota Eri, Hata Jumpei, Homma Keisuke, Zhu Bo, Hitomi Kiyotaka, Nakano Hideo

    JOURNAL OF BIOSCIENCE AND BIOENGINEERING   Vol. 121 ( 2 ) page: 147-153   2016.2

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    DOI: 10.1016/j.jbiosc.2015.06.003

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  4. In vitro generation of rabbit anti-Listeria monocytogenes monoclonal antibody using single cell based RT-PCR linked cell-free expression systems Reviewed

    Ojima-Kato Teruyo, Hashimura Dai, Kojima Takaaki, Minabe Shiori, Nakano Hideo

    JOURNAL OF IMMUNOLOGICAL METHODS   Vol. 427   page: 58-65   2015.12

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    DOI: 10.1016/j.jim.2015.10.001

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  5. Construction of a DNA Library on Microbeads Using Whole Genome Amplification Reviewed

    Kojima Takaaki, Zhu Bo, Nakano Hideo

    WHOLE GENOME AMPLIFICATION: METHODS AND PROTOCOLS   Vol. 1347   page: 87-100   2015

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    DOI: 10.1007/978-1-4939-2990-0_6

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  6. Bio-nanocapsules for oriented immobilization of DNA aptamers on aptasensors Reviewed

    Iijima Masumi, Yamada Yuki, Nakano Hideo, Nakayama Tsutomu, Kuroda Shun'ichi

    ANALYST   Vol. 147 ( 3 ) page: 489 - 495   2022.1

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Analyst  

    The oriented immobilization of sensing molecules (e.g., IgGs, receptors, lectins, and DNA aptamers) on sensor chips is particularly important for maximizing the potential of the sensing molecules, thereby enhancing the sensitivity and target-binding capacity of biosensors. We previously developed ∼30 nm bio-nanocapsules (ZZ-BNCs) consisting of the hepatitis B virus envelope L protein fused with the tandem form of protein A-derived IgG Fc-binding Z domain (ZZ-L protein). ZZ-BNC acts successfully as a scaffold, enhancing both the sensitivity and binding capacity of IgG, a Fc-fused receptor, and Fc-fused lectin to antigens, cytokines, and sugar chains through an oriented immobilization on a biosensor surface. To expand the versatility of ZZ-BNC, we modified ZZ-BNC by replacing the ZZ domain with a DNA-binding single-chain lambda Cro (scCro) domain, thereby developing scCro-BNC. The scCro-BNC was synthesized in yeast cells and homogeneously purified as ∼30 nm sized nanoparticles. In a quartz crystal microbalance, an scCro-BNC-coated sensor chip immobilized with thrombin-binding DNA aptamers showed an ∼5.5-fold higher thrombin-binding capacity and ∼6000-fold higher detection sensitivity than a sensor chip directly coated with DNA aptamers. In addition, the number of bound thrombin molecules per molecule of DNA aptamer increased by ∼7.8-fold with an scCro-BNC coating, consistent with the theoretical thrombin-binding capacity. Collectively, scCro-BNC was shown to perform as an ideal scaffold for maximizing the potential of the DNA aptamer by immobilizing it in an oriented manner. Facilitating a highly sensitive detection of various target molecules, these BNC-based scaffolds are expected to improve a wide range of biosensors while minimizing the number of sensing molecules required. This journal is

    DOI: 10.1039/d1an02278d

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  7. 無細胞タンパク質合成系を利用した迅速抗体スクリーニング技術開発と実用化 Invited

    加藤晃代, 中野秀雄, 兒島孝明, 永井里美

    日本生物工学会会誌   Vol. 99 ( 2 ) page: 62 - 67   2021.2

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    Authorship:Last author   Language:Japanese   Publishing type:Research paper (scientific journal)  

  8. Development of robust and rapid monoclonal antibody screening technology using cell-free protein synthesis

    Ojima-Kato T., Nakano H., Kojima T., Nagai S.

    Seibutsu-kogaku Kaishi   Vol. 99 ( 2 ) page: 62 - 67   2021

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    Language:Japanese   Publisher:Seibutsu-kogaku Kaishi  

    DOI: 10.34565/seibutsukogaku.99.2_62

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  9. Administration of Aspergillus oryzae suppresses DSS-induced colitis. International journal

    Ryo Nomura, Sho Tsuzuki, Takaaki Kojima, Mao Nagasawa, Yusuke Sato, Masayoshi Uefune, Yasunori Baba, Toshiya Hayashi, Hideo Nakano, Masashi Kato, Motoyuki Shimizu

    Food chemistry. Molecular sciences   Vol. 4   page: 100063 - 100063   2022.7

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    Aspergillus oryzae, a filamentous fungus, has long been used for the production of traditional Japanese foods. Here, we analyzed how A. oryzae administration affects the intestinal environment in mice. The results of 16S rRNA gene sequencing of the gut microbiota indicated that after the administration of heat-killed A. oryzae spores, the relative abundance of an anti-inflammatory Bifidobacterium pseudolongum strain became 2.0-fold greater than that of the control. Next, we examined the effect of A. oryzae spore administration on the development of colitis induced by dextran sodium sulfate in mice; we found that colitis was alleviated by not only heat-killed A. oryzae spores, but also the cell wall extracted from the spores. Our findings suggest that A. oryzae holds considerable potential for commercial application in the production of both traditional Japanese fermented foods and new foods with prebiotic functions.

    DOI: 10.1016/j.fochms.2021.100063

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  10. Structures of an engineered phospholipase D with specificity for secondary alcohol transphosphatidylation: Insights into plasticity of substrate binding and activation. Invited Reviewed International coauthorship

    Samantha, A., Damnjanović, J., Iwasaki, Y., Nakano, H., and Vrielink, A.

    Biochemical Journal   Vol. 478   page: 1749 - 1767   2021.5

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1042/BCJ20210117.

  11. Increasing cell-free gene expression yields from linear templates inEscherichia coliandVibrio natriegensextracts by using DNA-binding proteins Reviewed International coauthorship

    Bo Zhu, Rui Gan, Maria D. Cabezas, Takaaki Kojima, Robert Nicol, Michael C. Jewett, Hideo Nakano

    BIOTECHNOLOGY AND BIOENGINEERING   Vol. 117 ( 12 ) page: 3849 - 3857   2020.12

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:WILEY  

    In crude extract-based cell-free protein synthesis (CFPS), DNA templates are transcribed and translated into functional proteins. Although linear expression templates (LETs) are less laborious and expensive to generate, plasmid templates are often desired over polymerase chain reaction-generated LETs due to increased stability and protection against exonucleases present in the extract of the reaction. Here we demonstrate that addition of a double stranded DNA-binding protein to the CFPS reaction, termed single-chain Cro protein (scCro), achieves terminal protection of LETs. This CroP-LET (scCro-based protection of LET) method effectively increases superfolder green fluorescent protein (sfGFP) expression levels from LETs inEscherichia coliCFPS reactions by sixfold. Our yields are comparable to other strategies that provide chemical and enzymatic DNA stabilization inE. coliCFPS. Notably, we also report that the CroP-LET method successfully enhanced yields in CFPS platforms derived from nonmodel organisms. Our results show that CroP-LET increased sfGFP yields by 18-fold in theVibrio natriegensCFPS platform. With the fast-expanding applications of CFPS platforms, this method provides a practical and generalizable solution to protect linear expression DNA templates.

    DOI: 10.1002/bit.27538

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  12. Development of a dual monoclonal antibody sandwich enzyme-linked immunosorbent assay for the detection of swine influenza virus using rabbit monoclonal antibody by Ecobody technology Reviewed

    Daorung Sila-on, Phornnaphat Chertchinnapa, Yusuke Shinkai, Takaaki Kojima, Hideo Nakano

    JOURNAL OF BIOSCIENCE AND BIOENGINEERING   Vol. 130 ( 2 ) page: 217 - 225   2020.8

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    Authorship:Corresponding author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:SOC BIOSCIENCE BIOENGINEERING JAPAN  

    A dual monoclonal antibody sandwich enzyme-linked immunosorbent assay (mAb sandwich ELISA) has been developed using rabbit monoclonal antibodies generated by Ecobody technology, which includes the isolation of single B cells binding to a specific antigen, amplification of the heavy and light chains of these immunoglobulins, and expression of the fragment of antigen binding (Fab) by cell-free protein synthesis (CFPS). A rabbit was immunized with swine influenza virus (SIV) vaccine, from which single B cells binding to the antigen were isolated. Then, immunoglobulin mRNA was amplified from single cells by reverse transcription-polymerase chain reaction, followed by the attachment of a T7 promoter, appropriate tags, and a T7 terminator for the expression of the Fab portion by CFPS. By taking advantage of two different peptide tags fused to the same Fab, optimal combinations for coating Fab on assay plates and detecting Fab, both synthesized by CFPS, were investigated for mAb sandwich ELISA. Pairs of Fab detected 0.5 ng SIV in the assay. In summary, this result showed the applicability of Ecobody technology for a variety of immunodetection kits for high throughput analyses. (C) 2020, The Society for Biotechnology, Japan. All rights reserved.

    DOI: 10.1016/j.jbiosc.2020.03.003

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  13. A simple, real-time assay of horseradish peroxidase using biolayer interferometry

    Takaaki Kojima, Ayako Nakane, Bo Zhu, Almasul Alfi, Hideo Nakano

    Bioscience, Biotechnology, and Biochemistry   Vol. 83 ( 10 ) page: 1822 - 1828   2019.10

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    Language:Japanese   Publishing type:Research paper (scientific journal)   Publisher:Informa UK Limited  

    DOI: 10.1080/09168451.2019.1621156

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  14. Production of active manganese peroxidase in Escherichia coli by co-expression of chaperones and in vitro maturation by ATP-dependent chaperone release

    Almasul Alfi, Bo Zhu, Jasmina Damnjanović, Takaaki Kojima, Yugo Iwasaki, Hideo Nakano

    Journal of Bioscience and Bioengineering   Vol. 128 ( 3 ) page: 290 - 295   2019.9

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    Language:Japanese   Publishing type:Research paper (scientific journal)   Publisher:Elsevier BV  

    DOI: 10.1016/j.jbiosc.2019.02.011

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  15. Comprehensive investigation of the gene expression system regulated by an Aspergillus oryzae transcription factor XlnR using integrated mining of gSELEX-Seq and microarray data. Reviewed

    Oka H, Kojima T, Ihara K, Kobayashi T, Nakano H

    BMC genomics   Vol. 20 ( 1 ) page: 16   2019.1

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    Language:Japanese   Publishing type:Research paper (scientific journal)  

    DOI: 10.1186/s12864-018-5375-5

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  16. Acyl chain that matters: Introducing sn-2 acyl chain preference to a phospholipase D by protein engineering. Reviewed

    Jasmina Damnjanović, Hideo Nakano, Yugo Iwasaki

    Protein Eng. Des. Sel.   Vol. 32 ( 1 ) page: 1 - 11   2019.1

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:OXFORD UNIV PRESS  

    Phospholipase D (PLD) is an enzyme widely used for enzymatic synthesis of structured phospholipids (PLs) with modified head groups. These PLs are mainly used as food supplements and liposome ingredients. Still, there is a need for an enzyme that discriminates between PLs and lysoPLs, for specific detection of lysoPLs in various specimens and enzymatic synthesis of certain PLs from a mixed substrate. To meet this demand, we aimed at altering sn-2 acyl chain recognition of a PLD, leading to a variant enzyme preferably reacting on lysoPLs, by protein engineering. Based on the crystal structure of Streptomyces antibioticus PLD, W166 was targeted for saturation mutagenesis due to its strong interaction with the sn-2 acyl chain of the PL. Screening result pointed at W166R and W166K PLDs to selectively react on lysophosphatidylcholine (lysoPC), while not on PC. These variants showed a negative correlation between activity and sn-2 chain length of PL substrates. This behavior was not observed in the wild-type (WT)-PLD. Kinetic analysis revealed that the W166R and W166K variants have 7-10 times higher preference to lysoPC compared to the WT-PLD. Additionally, W166R PLD showed detectable activity toward glycero-3-phosphocholine, unlike the WT-PLD. Applicability of the lysoPC-preferring PLD was demonstrated by detection of lysoPC in the mixed PC/lysoPC sample and by the synthesis of cyclic phosphatidic acid. Structure model analyses supported the experimental findings and provided a basis for the structure model-based hypothesis on the observed behavior of the enzymes.

    DOI: 10.1093/protein/gzz019

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  17. SKIK-zipbody-alkaline phosphatase, a novel antibody fusion protein expressed in Escherichia coli cytoplasm

    Panwad Ritthisan, Teruyo Ojima-Kato, Jasmina Damnjanovic, Takaaki Kojima, Hideo Nakano

    JOURNAL OF BIOSCIENCE AND BIOENGINEERING   Vol. 126 ( 6 ) page: 705 - 709   2018.12

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    Language:Japanese   Publishing type:Research paper (scientific journal)   Publisher:SOC BIOSCIENCE BIOENGINEERING JAPAN  

    Antibody-enzyme fusion proteins have been used for various immunological detection techniques, such as ELISA, Western blotting and so on. The use of genetically-fused antibody-enzyme complexes has advantages over conventional chemical conjugation methods, as they require no complex chemical reactions and allow for the strict control of the number of enzymes fused with antibodies, resulting in a more stable performance of the bifunctional protein. Here, we describe efficient cytoplasmic soluble expression of an antigen-binding fragment (Fab) fused with Escherichia coli alkaline phosphatase (AP), N-terminal Ser-Lys-Ile-Lys (SKIK) tag that can improve the synthesis of the tagged protein, as well as leucine zipper (LZ) to enhance the association of the light chain and the heavy chain of Fab. Our results demonstrated that the SKIK-Fab-LZ-AP fusion was well expressed in E. coli oxidative cytoplasm in soluble form having both antigen-binding and AP activity, and was purified to homogeneity by two step column chromatography, suggesting that the combination of the SKIK tag and AP fusion can greatly increase the productivity and solubility of the Fab enzyme fusion in an E. coli cytoplasmic expression system. (C) 2018, The Society for Biotechnology, Japan. All rights reserved.

    DOI: 10.1016/j.jbiosc.2018.06.009

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  18. Rapid Generation of Monoclonal Antibodies from Single B Cells by Ecobody Technology

    Ojima-Kato Teruyo, Morishita Shiomi, Uchida Yoshino, Nagai Satomi, Kojima Takaaki, Nakano Hideo

    ANTIBODIES   Vol. 7 ( 4 )   2018.11

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    DOI: 10.3390/antib7040038

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  19. Spatial arrangement of proteins using scCro-tag: application for an in situ enzymatic microbead assay Reviewed

    Kojima Takaaki, Hata Jumpei, Oka Hiroya, Hayashi Kenta, Hitomi Kiyotaka, Nakano Hideo

    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY   Vol. 82 ( 11 ) page: 1911 - 1921   2018.11

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    Language:Japanese   Publishing type:Research paper (scientific journal)  

    DOI: 10.1080/09168451.2018.1501265

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  20. Zipbodyzyme: Development of new antibody-enzyme fusion proteins.

    Mori A, Ojima-Kato T, Kojima T, Nakano H

    Journal of bioscience and bioengineering     2018.2

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    DOI: 10.1016/j.jbiosc.2017.12.021

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  21. Pilot-scale whole-cell biocatalysis for the hydroxylation of cyclosporine derivative, FR901459, at higher concentrations by Lentzea sp 7887 using soybean flour as a novel substrate dispersant

    Yabutani Tetsuya, Shimizu Shiho, Nakano Hideo

    JOURNAL OF BIOSCIENCE AND BIOENGINEERING   Vol. 123 ( 1 ) page: 56 - 62   2017.1

  22. 'Zipbody' leucine zipper-fused Fab in E-coli in vitro and in vivo expression systems

    Ojima-Kato Teruyo, Fukui Kansuke, Yamamoto Hiroaki, Hashimura Dai, Miyake Shiro, Hirakawa Yuki, Yamasaki Tomomi, Kojima Takaaki, Nakano Hideo

    PROTEIN ENGINEERING DESIGN & SELECTION   Vol. 29 ( 4 ) page: 149-157   2016.4

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    DOI: 10.1093/protein/gzw001

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  23. Handmade microfluidic device for biochemical applications in emulsion

    Murzabaev Marsel, Kojima Takaaki, Mizoguchi Takuro, Kobayashi Isao, DeKosky Brandon J., Georgiou George, Nakano Hideo

    JOURNAL OF BIOSCIENCE AND BIOENGINEERING   Vol. 121 ( 4 ) page: 471-476   2016.4

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    DOI: 10.1016/j.jbiosc.2015.08.001

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  24. Directing positional specificity in enzymatic synthesis of bioactive 1-phosphatidylinositol by protein engineering of a phospholipase D Reviewed

    Damnjanovic Jasmina, Kuroiwa Chisato, Tanaka Hidetoshi, Ishida Ken, Nakano Hideo, Iwasaki Yugo

    BIOTECHNOLOGY AND BIOENGINEERING   Vol. 113 ( 1 ) page: 62-71   2016.1

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    DOI: 10.1002/bit.25697

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  25. Signal peptide optimization tool for the secretion of recombinant protein from Saccharomyces cerevisiae Reviewed

    Mori Akihiro, Hara Shoichi, Sugahara Tomohiro, Kojima Takaaki, Iwasaki Yugo, Kawarasaki Yasuaki, Sahara Takehiko, Ohgiya Satoru, Nakano Hideo

    JOURNAL OF BIOSCIENCE AND BIOENGINEERING   Vol. 120 ( 5 ) page: 518-525   2015.11

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    DOI: 10.1016/j.jbiosc.2015.03.003

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  26. Role of disulfide bond isomerase DsbC, calcium ions, and hemin in cell-free protein synthesis of active manganese peroxidase isolated from Phanerochaete chrysosporium Reviewed

    Ninomiya Ryoko, Zhu Bo, Kojima Takaaki, Iwasaki Yugo, Nakano Hideo

    JOURNAL OF BIOSCIENCE AND BIOENGINEERING   Vol. 117 ( 5 ) page: 652-657   2014.5

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    DOI: 10.1016/j.jbiosc.2013.11.003

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  27. Deletion of a Dynamic Surface Loop Improves Stability and Changes Kinetic Behavior of Phosphatidylinositol-Synthesizing Streptomyces Phospholipase D Reviewed

    Damnjanovic Jasmina, Nakano Hideo, Iwasaki Yugo

    BIOTECHNOLOGY AND BIOENGINEERING   Vol. 111 ( 4 ) page: 674-682   2014.4

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    DOI: 10.1002/bit.25149

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  28. A chromogenic substrate for solid-phase detection of phospholipase A(2) Reviewed

    Eba Chisato, Okano Aoi, Nakano Hideo, Iwasaki Yugo

    ANALYTICAL BIOCHEMISTRY   Vol. 447   page: 43-45   2014.2

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    DOI: 10.1016/j.ab.2013.11.007

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  29. Comprehensive Analysis of the DNA-Binding Specificity of an Aspergillus nidulans Transcription Factor, AmyR, by using a Bead Display System. Reviewed

    Wang, P., Kojima, T., Kobayashi, T. and Nakano, H.

    J. Biosci. Biotechnol. Biochem.   Vol. 76 ( 1 ) page: 1128-1134   2012.6

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  30. Comprehensive Analysis of the DNA-Binding Specificity of an Aspergillus nidulans Transcription Factor, AmyR, Using a Bead Display System

    Wang Panhui, Kojima Takaaki, Kobayashi Tetsuo, Nakano Hideo

    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY   Vol. 76 ( 6 ) page: 1128-1134   2012.6

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    DOI: 10.1271/bbb.110949

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  31. Emulsion Culture: A Miniaturized Library Screening System Based on Micro-droplets in an Emulsified Medium. Reviewed

    Kojima, T., Nagao, N., Ando, D., Ojima, T., Kawarasaki, Y., Kobayashi, I., Nakajima, M. and Nakano, H.

    J. Biosci. Bioeng.   Vol. 112   page: 299-303   2011.6

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  32. GLOBE:Analysis of DNA-Protein Interaction Analysis. Invited

    Kojima, T. and Nakano, H.

    Methods in Molecular Biology,   Vol. 687   page: 307-317   2011

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    DOI: 10.1007/978-1-60761-944-4_22

  33. High-throughput Screening of DNA Binding Sites for Transcription Factor AmyR from Aspergillus nidulans Using DNA Beads Display System. Reviewed

    Kojima, T., Hashimoto, Y., Kato, M., Kobayashi, T. and Nakano, H.

    J. Biosci. Bioeng.   Vol. 109 ( 6 ) page: 519-525.   2010.6

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  34. High-throughput screening of DNA binding sites for transcription factor AmyR from Aspergillus nidulans using DNA beads display system

    Kojima Takaaki, Hashimoto Yoko, Kato Masashi, Kobayashi Tetsuo, Nakano Hideo

    JOURNAL OF BIOSCIENCE AND BIOENGINEERING   Vol. 109 ( 6 ) page: 519-525   2010.6

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    DOI: 10.1016/j.jbiosc.2009.11.024

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  35. (2010) Directed Evolution of Angiotensin II-inhibiting Peptides Using Microbeads Display. Reviewed

    Gan, R., Furuzawa, S., Kojima, T., Kanie, K., Kato, R., Okochi, M., Honda, H. and Nakano, H.

    J. Biosci. Bioeng.   Vol. 109 ( 4 ) page: 411-417   2010.4

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  36. Synthesis of phosphatidylinositols having various inositol stereoisomers by engineered phospholipase D Reviewed

    Akari Ozaki, Atsushi Masayama, Hideo Nakano, Yugo Iwasaki

    J. Biosci. Bioeng.   Vol. 109   page: 337-340   2010.4

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  37. (2010) In vitro generation of anti-hepatitis B monoclonal antibodies from a single plasma cell using single-cell RT-PCR and cell-free protein synthesis. Reviewed

    Sabrina, Y., Ali, M. and Nakano, H.

    J. Biosci. Bioeng.   Vol. 109 ( 1 ) page: 75-82   2010.1

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    Language:English   Publishing type:Research paper (scientific journal)  

  38. *Microbeads display of proteins using emulsion PCR and cell-free protein synthesis. Reviewed

    Gan, R, Yamanaka, Y, Kojima, T, and Nakano, H.

    Biotechnol. Prog.   Vol. 24   page: 1107-1114   2008.1

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  39. Streptomyces phospholipase D mutants with altered substrate specificity capable of phosphatidylinositol synthesis. Reviewed

    Masayama, A., Takahashi, T., Tsukada, K., Nishikawa, S., Takahashi, R., Adachi, M., Koga, K., Suzuki, A., Yamane T., Nakano, H., and Iwasaki, Y.

    Chembiochem   Vol. 9   page: 974-981   2008.1

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  40. Direct separation of monoacylglycerol isomers by enantioselective high-performance liquid chromatography. Reviewed

    Deng, L., Nakano, H. and Iwasaki, Y.

    J. Chromatogr. A.   Vol. 1198-1199   page: 67-72   2008.1

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  41. Direct separation of regioisomers and enantiomers of monoacylglycerols by tandem column high-performance liquid chromatography

    Deng Li, Nakano Hideo, Iwasaki Yugo

    JOURNAL OF CHROMATOGRAPHY A   Vol. 1165 ( 1-2 ) page: 93-99   2007.9

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    DOI: 10.1016/j.chroma.2007.07.073

    Web of Science

  42. 無細胞タンパク質合成系を用いた進化分子工学システムの構築と応用.

    兒島孝明,中野秀雄

    生化学   Vol. 79   page: 239 -246   2007.4

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  43. Development and application of evolutionary molecular engineering systems using cell-free protein synthesis

    Kojima Takaaki, Nakano Hideo

    SEIKAGAKU   Vol. 79 ( 3 ) page: 239-246   2007.3

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    Web of Science

  44. Direct separation of regioisomers and enantiomers of monoacylglycerols by tandem column high-performance liquid chromatography. Reviewed

    Deng, L., Nakano, H. and Iwasaki, Y.

    J. Chromatogr.   Vol. 1198-1199   page: 93-99   2007.1

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  45. Creation of novel enantioselective lipases by SIMPLEX. Invited

    Koga, Y., Yamane, T. and Nakano, H.

    Methods Mol. Biol.   Vol. 375   page: 165-182   2007.1

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  46. SIMPLEX: single-molecule PCR-linked in vitro expression: a novel method for high-throughput construction and screening of protein libraries. Invited

    Rungpragayphan, S., Yamane, T. and Nakano, H.

    Methods Mol. Biol.   Vol. 375   page: 79-94   2007.1

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  47. High-speed and high sensitive immunochemical detection using pillar arrays Reviewed

      Vol. 126 ( 8 ) page: 486-491   2006.8

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  48. In vitro selection of DNA binding sites for transcription factor, PhaR, from Paracoccus denitrificans using genetic library on microbeads and flow cytometry

    Kojima Takaaki, Yamane Tsuneo, Nakano Hideo

    JOURNAL OF BIOSCIENCE AND BIOENGINEERING   Vol. 101 ( 5 ) page: 440-444   2006.5

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    DOI: 10.1263/jbb.101.440

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  49. Generation of Monoclonal Antibodies Using Simplified Single-Cell Reverse Transcription-Polymerase Chain Reaction and Cell-Free Protein Synthesis. Reviewed

    Muhamad Ali, Hitomi, K. and Nakano, H.

    Journal of Bioscience and Bioengineering,   Vol. 101,   page: 284-286   2006.4

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  50. *A Novel Strategy for Generation of Monoclonal Antibodies from Single B Cells Using RT-PCR Technique and in Vitro Expression. Reviewed

    XiuPing Jiang, Suzuki, H., Hanai, Y., Wada, F., Hitomi, K., Yamane, T. and Nakano, H.

    Biotechnol. Prog.   Vol. 22   page: 979-988   2006.4

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  51. In vitro selection of DNA binding sites for transcription factor, PhaR, from Paracoccus denitrificans using genetic library on microbeads and flow cytometry. Reviewed

    Kojima, T., Yamane, T. and Nakano, H.

    Journal of Bioscience and Bioengineering   Vol. 101   page: 440-444.   2006.3

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  52. Improvements in the cell-free production of functional antibodies using cell extract from protease-deficient Escherichia coli mutant. Reviewed

    Muhamad Ali, Suzuki, H., Fukuba, T., Jiang, X., Nakano, H. and Yamane, T.

    Journal of Bioscience and Bioengneering   Vol. 99   page: 181-186.   2005

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  53. Novel Strategy for Protein Exploration: High-throughput Screening Assisted with Fuzzy Neural Network, Reviewed

    R. Kato, H. Nakano, H. Konishi, K. Kato, Y. Koga, T. Yamane, T. Kobayashi and H. Honda

    Journal of Molecular Biology   Vol. 35 ( 3 ) page: 683-692   2005

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  54. *PCR amplification from single DNA molecules on magnetic beads in emulsion: application for high-throughput screening of transcription factor targets, Reviewed

    T. Kojima, Y. Takei, M. Ohtsuka, Y. Kawarasaki, T. Yamane, and H. Nakano,

    Nucleic Acids Research   Vol. 33   page: e15   2005

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  55. Mass enhancement cell-free protein synthesis using S30 extract from Escherichia coli growing fast at 42 degrees on amino acid-enriched medium. Reviewed

    Yamane, T., Ikeda, Y., Nagasaka, T. and Nakano, H.

    Biotechnology Progress   Vol. 21   page: 608-613.   2005

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  56. 無細胞タンパク質合成系を用いたハイスループットスクリーニング技術の開発と応用

    日本農芸化学会誌   Vol. 78 ( 5 ) page: 483-486   2004

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  57. A picoliter chamber array for cell-free protein synthesis Reviewed

    Takeshi Kinpara, Ryota Mizuno, Yuji Murakami, Masaki Kobayashi, Shouhei Yamamura, Quamrul Hasan, Yasutaka Morita, Hideo Nakano, Tsuneo Yamane, Eiichi Tamiya

    Journal of Biochemistry   Vol. 136 ( 2 ) page: 149-153   2004

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  58. SIMPLEX法によるリパーゼのタンパク質工学

    山根恒夫、中野秀雄

    日本農芸化学会誌   Vol. 78   page: 751-753   2004

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  59. Rapid screening for affinity-improved scFvs by means of single-molecule-PCR-linked in vitro expression. Reviewed

    Rungpragayphan, S., Haba, M., Nakano, H. and Yamane, T.

    Journal of Molecular Catalysis B: Enzymatic,   Vol. 28   page: 223-228   2004

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  60. Cell-free protein synthesis systems: Increasing their performance and applications.

    Nakano, H., Kawarasaki, Y., Yamane, T.

    Advances in Biochemical Engineering/Biotechnology   Vol. 90   page: 135-149   2004

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  61. SIMPLEX法によるタンパク質ライブラリーの構築 Invited

    科学と工業   Vol. 78 ( 6 ) page: 316-321   2004

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  62. *Inverting enantioselectivity of Burkholderia cepacia KWI-56 lipase by combinatorial mutation and high-throughput screening using single-molecule PCR and in vitro expression. Reviewed

    Journal of Molecular Biology   Vol. 331 ( 3 ) page: 585-592   2003.1

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  63. Improvement of H 2O2stability of manganese peroxidase by combinatorial mutagenesis and high-throughput screening using in vitro expression with protein disulfide isomerase. Reviewed

    Protein Engineering   Vol. 16 ( 6 ) page: 423-428   2003

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  64. PCR-linked in vitro expression: a novel system for high-throughput construction and screening of protein libraries. Reviewed

    FEBS Lett   Vol. 540   page: 147-150   2003

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  65. Cloning and in vitro and in vivo expression of plant glutathione S-transferase zeta class gene. Reviewed

    Journal of Bioscience and Bioengineering   Vol. 95 ( 6 ) page: 594-600   2003

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  66. Stabilization of Affinity-Tagged Recombinant Protein during/after Its Production in a Cell-Free System Using Wheat-Germ Extract. Reviewed

    Journal of Bioscience and Bioengineering   Vol. 95 ( 3 ) page: 209-214   2003

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  67. *無細胞タンパク質合成系の高度化と応用 Invited

    生物工学会誌   Vol. 81 ( 2 ) page: 71-76   2003

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  68. High-throughput, cloning-independent protein library construction by combining single-molecule DNA amplification with in vitro expression. Reviewed

    Journal of Molecular Biology   Vol. 318   page: 395-405   2002

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  69. In vitro combinatorial mutagenesis of the 65th and 22nd positions of the green fluorescent protein of Aequarea victoria. Reviewed

    Biotechnology and Bioprocess Engineering   Vol. 7   page: 1-6   2002

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  70. Modifying the chain length selectivity of the lipase from Burkholderia cepacia KWI-56 through in vitro combinatorial mutagenesis in the substrate binding site. Reviewed

    Protein Engineering   Vol. 15   page: 147-152   2002

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  71. Reduction of protein degradation by use of protease-deficient mutants in cell-free protein synthesis system of Escherichia coli. Reviewed

    Journal of Bioscience and Bioengineering   Vol. 93 ( 2 ) page: 151-156   2002

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  72. In vitro construction and screening of Burkholderia cepacia lipase library using single-molecule PCR and cell-free protein synthesis. Reviewed

    Journal of Bioscience and Bioengineering   Vol. 94 ( 1 ) page: 84-86   2002

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  73. Expression of Fab fragment of catalytic antibody 6D9 in an Escherichia coli in vitro coupled transcription/translation system. Reviewed

    FEBS Letters,   Vol. 514   page: 290-294   2002

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  74. 無細胞蛋白質合成系による新機能分子の創出

    化学と生物   Vol. 40 ( 8 ) page: 533-538   2002

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    Language:Japanese  

  75. 無細胞タンパク質合成系の進歩と応用

    現代化学   Vol. 370   page: 66-72   2002

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  76. 無細胞系によるタンパク質創製システム

    バイオインダストリー   Vol. 18 ( 6 ) page: 42-48   2001

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  77. PhaR, a protein of unknown function conserved among short-chain-length polyhydroxyalkanoic acids producing bacteria, is a DNA-binding protein and represses Paracoccus denitrificans phaP expression in vitro. Reviewed

    FEMS Microbiol Lett.   Vol. 200 ( 1 ) page: 9-15   2001

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  78. Dosage effect of minor arginyl- and isoleucyl-tRNA on protein synthesis in an E.coli in vitro coupled transcription/translation system. Reviewed

    Journal of Bioscience and Bioengineering   Vol. 91 ( 1 ) page: 53-57   2001

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  79. "Good-bye“大腸菌"":無細胞タンパク質合成系の挑戦"

    中野秀雄

    バイオサイエンスとインダストリー   Vol. 58   page: 11-15   2000.1

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  80. A trimmed virul cap-independent translation enhancing sequence for rapid in vitro gene expression.

    "Kawarasaki,Y. Kasahara,S., Kodera,N., Shinbata,T., Sekiguchi,S., Nakano, H.,and Yamane"

    Biotechnology Progress   Vol. 16   page: 517-521   2000.1

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  81. Importance of disulfide bridge formation on folding of phospholipase D from Streptomyces antibioticus. Reviewed

    Journal of Bioscience and Bioengineering   Vol. 89 ( 5 ) page: 506-508   2000

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  82. Single-step Single-Molecule PCR of DNA with a homo-priming sequence using a single primer and hot-startable DNA polymerase Reviewed

    Journal of Bioscience and Bioengineering   Vol. 90   page: 456   2000

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  83. In vitro analysis of roles of a disulfied bridge and a calcium binding site in activation of Pseudomonas sp. strain KWI-56 lipase. Reviewed

    Journal of Bacteriology   Vol. 182   page: 295-302   2000

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  84. Amalyses of a polybydroxy alkanoic acid granule-associated 16-kilodalton protein and its patatire regulator in the pha locus of Paracoccus denitrificans. Reviewed

    Journal of Bacteriology   Vol. 121 ( 9 ) page: 2914-2921   1999

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  85. Efficient coupled transcrption /trornslation for PCR template by a hollow fiber membra in bioreactar. Reviewed

    Biotechnology and Bioengineering   Vol. 64 ( 2 ) page: 194-199   1999

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  86. Modulator-mediated synthesis of active lipase of Pseudomonas sp. 109 by Escherichia coli cell-free coupled transcription/translation system. Reviewed

    Journal of Bioscience and Bioengineering   Vol. 88   page: 605-609   1999

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  87. Purification, characterization and gene cloning of 6-hydroxy nicotinate 3-monooyygenase from Pseadomonas flaovescene 7N5. Reviewed

    European Journal of Biachemistry   Vol. 260   page: 120-126   1999

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  88. 無細胞たん白質合成

    山根恒夫、中野秀雄、河原崎泰昌

    ケミカル・エンジニアリング   Vol. 43   page: 198-206   1998.1

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  89. Two distinct phosphatidylinositol-specific phospholipase Cs from Streptomyces antibioticus.

    "Iwasaki, Y., Tsubouchi, Y., Ichihashi, A., Nakano, H., Kobayashi, T., Ikezawa, H., and Yamane, T.,"

    Biochemica Biophysica Acta   Vol. 1391   page: 52-66   1998.1

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  90. In vitro method for the generation of protein libraries using PCR amplification of a single DNA molecule and coupled transciptin /translation. Reviewed

    Nucleic Acids Research   Vol. 26 ( 19 ) page: 4339-4346   1998

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  91. Cell-free protein synthesis systems.

    Biotechnolosy Advances   Vol. 16 ( 2 ) page: 367-384   1998

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  92. Phosphatase-immunodepleted cell-free protein synthesis system. Reviewed

    Journal of Biotechnology   Vol. 61   page: 199-202   1998

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  93. Insertion of stabilizing loci in vectors of T7 RNA polymerase-mediated scherichia coliexpression systems: A case study on the plasmids involving foreign phospholipase D gene

    "Mishima, N., Mizumoto, K., Iwasaki, Y., Nakano, H. and Yamane, T.,"

    Biotechnology Progress   Vol. 13   page: 864-868   1997.1

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  94. Accumulation of translational inhibitors during multi-hour cell-free protein synthesis reaction using rabbit reticalocy of lysate. Reviewed

    Journal of Fermentation and Bioengineering   Vol. 83 ( 5 ) page: 470-473   1997

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  95. Recent advances in cell-free protein synthesis towards a protein biosynthesizer Invited Reviewed

    "Nakano, H., Kawarasaki, Y., and Yamane, T."

    Enzyme Engineering XIII (The Annals of the New York Academy of Sciences)   Vol. 799   page: 406-412   1996.1

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  96. Highly productive cell-free protein synthesis system using coensed wheat-germ extract

    "Nakano, H., Tanaka, T., Kawarasaki, Y. and Yamane, T.,"

    Journal of Biotechnology   Vol. 46   page: 275-282   1996.1

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  97. Highly productive cell-free protein synthesis system using condensed wheat-germ extract. Reviewed

    Journal of Biotechnolosy   Vol. 46   page: 275-282   1996

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  98. Purification and some properties of serm acid phosphatases. Reviewed

    Plant Science   Vol. 119   page: 67-77   1996

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  99. 無細胞系での蛋白質大量合成に向けて. ―─ 遺伝子翻訳機の構築 ――

    中野秀雄、山根恒夫

    バイオサイエンスとインダストリー   Vol. 53   page: 967-969   1995.1

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  100. A long-lived batch reaction system of cell-free protein synthesis

    "Kawarasaki, Y., Kawai, T., Nakano, H. and Yamane, T.,"

    Analytical Biochemistry   Vol. 226   page: 320-324   1995.1

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  101. n VitroProtein Biosynthesis Using Ribosome and Foreign mRNA - An Approach to Construct a Protein Biosynthesizer -

    "Yamane, T., Kawarasaki, Y. and Nakano, H."

    Enzyme Engineering XII (The Annals of the New York Academy of Sciences)   Vol. 750   page: 146-157   1995.1

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  102. A long-lived batch reaction system of cell free protein synthsis. Reviewed

    Analytical Biochemistry   Vol. 226 ( 2 ) page: 320-324   1995

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  103. In Vitro Protein Biosynthesis Using Ribosome and Foreign mRNA An Approach to Construct a Protein Biosynthesizer. Reviewed

    Enzyme Engineering XII   Vol. 750   page: 146-157   1995

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  104. Extracellular production of phospholipase D of Streptmyces antibioticus by recombinant Escherichia coli. Reviewed

    Journal of Fermentation and Bioengineering   Vol. 79 ( 5 ) page: 417-421   1995

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  105. High speed polymerase chain reaction in Constant flow. Reviewed

    Bioscience, Biotechnology and Biochemistry   Vol. 58 ( 2 ) page: 349-352   1994

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  106. An increased rate of cell-free protein synthesis by condenleing wheat-gem extuet with ultrafiltration menbranes. Reviewed

    Bioscience, Biotechnology and Biochemistry   Vol. 58 ( 4 ) page: 631-634   1994

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  107. Role of cystein residues in esterose from Bacillus stearothermophilus and increasing its thermostability by the replacement of cysteine. Reviewed

    Applied Microbiology and Biotechnology   Vol. 40 ( 3 ) page: 664-668   1994

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  108. PCRの高速化

    中野秀雄、山根恒夫

    蛋白質 核酸 酵素   Vol. 39   page: 2113-2120   1994

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  109. Secretion of a Cytoplasmic Protein Using Escherichia coli α-Hemolysin Membrane Transport System Reviewed

      Vol. 17   page: 3   1991

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  110. シグナル配列によらないタンパク質の膜透過系

    BIOmedica   Vol. 6   page: 186-190   1991

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Books 13

  1. 新版生物反応工学

    中野秀雄、岩崎雄吾、山根恒夫、河原崎泰昌、加藤雅士、志水元亭( Role: Joint author)

    産業図書株式会社  2016.9  ( ISBN:978-4-7828-2617-1 C

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    Total pages:275   Language:Japanese Book type:Textbook, survey, introduction

  2. Creation of Novel Enantioselective Lipases by SIMPLEX. In Vitro Transcription and Translation Protocols

    Koga, Y., Yamane, T. and Nakano, H.( Role: Joint author)

    Humana Press,  2007.4 

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    Language:English

  3. SIMPLEX: Single-Molecule PCR-Linked In Vitro Expression. In Vitro Transcription and Translation Protocols

    Rungpragayphan, S., Yamane, T. and Nakano, H.( Role: Joint author)

    Humana Press  2007.4 

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    Language:English

  4. 酵素の光学選択性に関与する基質認識部位の網羅的解析

    中野 秀雄( Role: Joint author)

    [出版者不明]  2007 

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  5. バイオプロダクション

    化学工学会バイオ部会編( Role: Joint author)

    コロナ社  2006.5 

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    Language:Japanese

  6. SIMPLEX法の開発と進化分子工学への応用. コンビナトリアル・バイオエンジニアリングの最前線 監修植田充美

    今村千絵、中野秀雄( Role: Joint author)

    シーエムシー出版  2004 

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    Language:Japanese

  7. In vitro expression of proteins with disulfide bridges and its application for high-throughput screening system. Cell-free protein expression ed. James R. Swartz

    Hideo Nakano and Tsuneo Yamane( Role: Joint author)

    Springer  2003 

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    Language:English

  8. マイクロリアクタアレイによる超高密度タンパク質・ペプチド分子ライブラリの構築

    中野 秀雄( Role: Joint author)

    [出版者不明]  2003 

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  9. 無細胞タンパク質合成系による蛋白質工学. 化学フロンティア「コンビナトリアル・バイオエンジニアリング」( 編:植田充美,近藤昭彦)"

    中野秀雄,古賀雄一( Role: Joint author)

    化学同人  2002 

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    Language:Japanese

    無細胞タンパク質合成系によるタンパク質工学

  10. 無細胞タンパク質合成系の最近の進歩 化学フロンティア『コンビナトリアル・バイオエンジニアリング』植田、近藤編

    中野秀雄( Role: Joint author)

    化学同人  2002 

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    Language:Japanese

    無細胞蛋白質合成系の最近のシンポ

  11. 日本生化学会編基礎生化学実験法

    中野秀雄( 編:)( Role: Joint author)

    東京化学同人  2001 

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    Language:Japanese

    試験管内での発現

  12. 高効率小麦胚芽タンパク質生合成系,

    遺伝子発現研究法(学会出版センター)  2000 

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    Language:Japanese

  13. 無細胞分子進化システムの構築

    中野 秀雄( Role: Joint author)

    [出版者不明]  1999 

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MISC 9

  1. タンパク質生産量を増大させるN末端SKIKペプチドタグ配列に関する研究

    古川裕貴, 加藤晃代, 中野秀雄, 中野秀雄

    日本農芸化学会中部支部例会講演要旨集(Web)   Vol. 187th   2020

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  2. タンパク質生産量を増大させるN末端SKIKペプチドタグ配列に関する研究

    古川裕貴, 加藤晃代, 加藤晃代, 中野秀雄, 中野秀雄

    日本生物工学会大会講演要旨集   Vol. 71st   2019

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  3. モノクローナル抗体迅速探索プラットフォーム技術の社会実装

    中野秀雄, 中野秀雄, 加藤晃代, 大内将司, 兒島孝明

    日本生物工学会大会講演要旨集   Vol. 71st   2019

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  4. 再構成型無細胞タンパク質合成系を用いた抗体-酵素融合タンパク質(Zipbodyzyme)の効率的合成

    伊藤玲奈, ALMASL Alfi, 加藤晃代, 兒島孝明, 中野秀雄

    日本生物工学会大会講演要旨集   Vol. 71st   2019

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  5. 麹菌摂取による宿主腸内細菌叢の改善および大腸炎の緩和

    兒島孝明, 志水元亨, 馬場保徳, 中野秀雄, 加藤雅士

    日本生物工学会大会講演要旨集   Vol. 71st   2019

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  6. シングルB細胞由来モノクローナル抗体の迅速取得と大腸菌による生産

    加藤晃代, 兒島孝明, 中野秀雄

    日本農芸化学会大会講演要旨集(Web)   Vol. 2018   2018

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  7. シングルB細胞由来モノクローナル抗体の迅速取得と大腸菌による生産

    加藤晃代, 兒島孝明, 田村廣人, 中野秀雄

    化学工学会秋季大会研究発表講演要旨集(CD-ROM)   Vol. 49th   2017

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  8. モノクローナル抗体取得技術の新展開 より良い抗体をより早く

    加藤晃代, 加藤晃代, 中野秀雄

    化学と生物   Vol. 55 ( 7 )   2017

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  9. 大腸菌発現システムを用いたシングルヒトB細胞からの抗乳酸菌ヒトモノクローナル抗体取得

    内田由乃, 加藤晃代, 兒島孝明, 中野秀雄

    日本農芸化学会大会講演要旨集(Web)   Vol. 2017   2017

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Presentations 43

  1. 無細胞タンパク質合成系を用いたモノクローナル抗体ハイスループットスクリーニング技術の開発と社会実装 Invited

    Hideo Nakano

    日本ビタミン学会第72回大会  2020.9.1  日本ビタミン学会

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    Event date: 2020.9

    Language:Japanese   Presentation type:Symposium, workshop panel (nominated)  

    Venue:web   Country:Japan  

  2. In vitro generation of Hepatitis B Monoclonal Antibody From Single Plasma Cells.

    Yunita Sabrina, Muhamad Ali, and Nakano, H

    日本農芸化学会2007年度大会 

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    Event date: 2007.3

    Language:English   Presentation type:Oral presentation (general)  

    Country:Japan  

  3. 特定蛋白質間相互作用の選択的破壊法の開発.

    池内暁紀,神谷拓摩,山根恒夫,中野秀雄,河原崎泰昌

    日本農芸化学会2007年度大会 

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    Event date: 2007.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  4. Direct separation of regio-and enantiomeric isomers of monoglycerides by a tandem column HPLC.

    Deng Li, Iwasaki, Y., and Nakano, H.

    日本農芸化学会2007年度大会 

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    Event date: 2007.3

    Language:English   Presentation type:Oral presentation (general)  

    Country:Japan  

  5. Microbeads Display of Proteins Using Emulsion PCR and Cell-free Protein Synthesis.

    Gan, R, Yamanaka, Y., and Nakano, H.

    日本農芸化学会2007年度大会 

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    Event date: 2007.3

    Language:English   Presentation type:Oral presentation (general)  

    Country:Japan  

  6. マイクロチャネル(MC)乳化法を用いたエマルジョンPCRによるマイクロビーズへのDNAライブラリーの構築.

    杉浦一輝,小林 功,中嶋光敏,中野秀雄:

    日本農芸化学会2007年度大会 

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    Event date: 2007.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  7. Altering substrate specificity of phospholipase D by directed evolution. International conference

    The Japan-Italy Symposium of New Trends in Enzyme Science and Technology, 

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    Event date: 2006.11

    Language:English   Presentation type:Poster presentation  

    Country:Japan  

  8. Protein engineering of lipase using cell-free protein synthesis system and information technology. International conference

    Japan-Italy Symposium of New Trends in Enzyme Science and Technology, 

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    Event date: 2006.11

    Language:English   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  9. Direct separation of regio-and enantiomeric isomers of monoglycerides by a tandem column HPLC.

    Deng Li, Iwasaki, Y., and Nakano, H.:

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    Event date: 2006.10

    Language:English   Presentation type:Poster presentation  

    Country:Japan  

  10. マイクロビーズ上での分子ディスプレイ法を用いたDNA-転写因子複合体検出法.

    橋本陽子,兒島孝明,加藤雅士,小林哲夫,中野秀雄

    日本農芸化学会中部支部例会 

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    Event date: 2006.10

    Language:Japanese   Presentation type:Poster presentation  

    Country:Japan  

  11. 糸状菌β―ガラクトシダーゼをレポーターとする半定量的酵母2ハイブリッド系の開発

    小島晃代,神谷拓摩,池内暁紀,中野秀雄,河原崎泰昌

    日本農芸化学会中部支部例会 

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    Event date: 2006.10

    Language:Japanese   Presentation type:Poster presentation  

    Country:Japan  

  12. Exploring novel enantio-selective lipases by high-throughput screening with the aid of fuzzy neural network analysis International conference

    The Ninth Japan-China-Korea Joint Symposium on Enzyme Engineering 

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    Event date: 2006.10

    Language:English   Presentation type:Oral presentation (general)  

    Country:Japan  

  13. Altering substrate specificity of phospholipase D by directed evolution. International conference

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    Event date: 2006.10

    Language:English   Presentation type:Poster presentation  

    Country:Japan  

  14. A Novel Compact Disk-like Microfluidic Device with Micropillars for Enzyme-linked Immunosorbent Assay.

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    Event date: 2006.9

    Language:English   Presentation type:Oral presentation (general)  

    Country:Japan  

  15. 無細胞蛋白質合成系を用いたリパーゼの蛋白質工学

    日本生物工学会平成18年度大会, 

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    Event date: 2006.9

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

    中野秀雄,山根恒夫

  16. 位置特異的アミノ酸飽和変異による2-デオキシリボース-5-リン酸アルドラーゼの基質特異性改変.

    木村昌博,則武智哉,河原崎泰昌,岩崎雄吾,吉田洋一,中野秀雄

    日本生物工学会平成18年度大会 

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    Event date: 2006.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  17. 特定蛋白質間相互作用の選択的破壊法の開発.

    池内暁紀,神谷拓摩,山根恒夫,中野秀雄,河原崎泰昌

    日本生物工学会平成18年度大会 

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    Event date: 2006.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  18. フローサイトメトリーを用いた転写因子AmyR-DNA複合体の検出法。

    橋本陽子、兒島孝明、加藤雅士、小林哲夫、中野秀雄

    日本農芸化学会2006年度大会 

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    Event date: 2006.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  19. 無細胞タンパク質合成系の高度化と応用

    中野秀雄

    日本農芸化学会2006年度大会 

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    Event date: 2006.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  20. ホスファチジルイノシトール合成活性を有する変異型ホスホリパーゼDの創製。

    高橋哲也、山根恒夫、中野秀雄、岩崎雄吾

    日本農芸化学会2006年度大会 

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    Event date: 2006.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  21. エマルジョンPCRを用いたマイクロビーズ上での分子ディスプレイ法の開発

    山中友美子、兒島孝明、山根恒夫、中野秀雄

    日本農芸化学会2006年度大会 

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    Event date: 2006.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  22. Protein-protein interaction network in Yeast kinetochore.

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    Event date: 2006.3

    Language:English   Presentation type:Oral presentation (general)  

    Country:Japan  

  23. 糸状菌β-ガラクトシダーゼを相互作用レポーターとする半定量的酵母2ハイブリッド系の開発。

    小島晃代、河原崎泰昌、神谷拓摩、池内暁紀、中野秀雄

    日本農芸化学会2006年度大会 

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    Event date: 2006.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  24. :Exhaustive identification of interaction domains using a novel high-throughput method: The first step toward interaction targeting. International conference

    PACIFICHEM 2005 

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    Event date: 2005.12

    Language:English   Presentation type:Poster presentation  

  25. Novel techniques using PCR and cell-free protein synthesis systems for combinatorial bioengineering. International conference

    PACIFICHEM 2005 

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    Event date: 2005.12

    Language:English   Presentation type:Oral presentation (general)  

  26. PCR amplification from single DNA molecule on magnetic bead in emulsion: Application for high-throughput screening of transcription factor targets. International conference

    PACIFICHEM 2005 

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    Event date: 2005.12

    Language:English   Presentation type:Poster presentation  

  27. Enzymatic preparation of enantiomerically pure2,3-diacylglycerol.

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    Event date: 2005.11

    Language:English   Presentation type:Oral presentation (general)  

    Country:Japan  

  28. 構造脂質の酵素合成

    岩崎雄吾、ピヤティラウォンウィーラ、中野秀雄、山根恒夫:

    日本生物工学会平成17年度大会 

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    Event date: 2005.11

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  29. 進化工学的手法を用いた出芽酵母キネトコアDam1複合体のサブユニット間相互作用領域の同定

    神谷拓摩、池内暁紀、河原崎泰昌、中野秀雄、山根恒夫

    日本生物工学会平成17年度大会 

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    Event date: 2005.11

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  30. Generation and Screening of Monoclonal Antibodies Using Single-Step Single Cell RT-PCR linked in vitro Expression

    Muhamad Ali, Hitomi, K., and Nakano, H.

    日本生物工学会平成17年度大会 

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    Event date: 2005.11

    Language:English   Presentation type:Oral presentation (general)  

    Country:Japan  

  31. W/Oエマルジョン内での固相1分子PCR無細胞蛋白質合成系を用いた蛋白質間相互作用検出法の開発。

    山中友美子、兒島孝明、山根恒夫、中野秀雄

    日本生物工学会平成17年度大会 

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    Event date: 2005.11

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  32. マイクロビーズ上での遺伝子ライブラリーを用いたDNA 結合タンパク質の結合DNA領域のハイスループットスクリーニング法の開発。

    兒島孝明、武井義明、山根恒夫、中野秀雄

    日本生物工学会平成17年度大会 

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    Event date: 2005.11

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  33. 位置特異的アミノ酸飽和変異による2-デオキシリボース-5-リン酸アルドラーゼの基質特異性改変(2)。

    木村昌博、則武智哉、河原崎泰昌、岩崎雄吾、吉田洋一、中野秀雄

    日本生物工学会平成17年度大会 

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    Event date: 2005.11

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  34. : Generation and Screening of Monoclonal Antibodies Using Single-Step Single Cell RT-PCR linked in vitro Expression.

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    Event date: 2005.10

    Language:English   Presentation type:Poster presentation  

    Country:Japan  

  35. A novel approach for generation and screening of monoclonal antibody by single cell RT-PCR and in vitro expression.

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    Event date: 2005.3

    Language:English   Presentation type:Poster presentation  

    Country:Japan  

  36. W/Oエマルジョン内1分子固相PCR法の開発。

    武井義明、兒島孝明、民谷栄一、山根恒夫、中野秀雄

    日本農芸化学会2005年度大会 

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    Event date: 2005.3

    Language:Japanese  

  37. ビーズディスプレイ法によるDNA結合タンパク質の結合DNA領域のスクリーニング法の開発。

    兒島孝明、武井義明、山根恒夫、中野秀雄

    日本農芸化学会2005年度大会 

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    Event date: 2005.3

    Language:Japanese   Presentation type:Poster presentation  

    Country:Japan  

  38. 有機鎖修飾メソポーラスシリカに固定化されたリパーゼの活性安定性。

    森岡幸、加藤且也、斎藤隆雄、Sindhu Seelan、横川善之、高橋治雄、中野秀雄、山根恒夫

    第8回生体触媒化学シンポジウム 

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    Event date: 2004.12

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  39. 中野秀雄、山根恒夫:SIMPLEX法による新規蛋白質の創製。

    第27回日本分子生物学会年会 

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    Event date: 2004.12

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  40. Exploring novel enantio-selective lipases by high-throughput screening with the aid of fuzzy neural network analysis. International conference

    The 10th APCChe congress 

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    Event date: 2004.11

    Language:English   Presentation type:Oral presentation (general)  

    Country:Japan  

  41. SIMPLEXを用いた新規単鎖抗体ライブラリーの構築とスクリーニング

    山中友美子、Rungpragayphan Suang、円谷 健、藤井郁雄、中野秀雄、山根恒夫

    日本生物工学会平成16年度大会 

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    Event date: 2004.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  42. SIMPLEX法により得られた光学選択性の反転した変異リパーゼ群の解析

    加賀哲也、中野秀雄、小西宏幸、古賀雄一、加藤克也、山根恒夫

    日本生物工学会平成16年度大会 

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    Event date: 2004.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  43. Creation of novel proteins by SIMPLEX. International conference

    3rd Japan-Korea Workshop on Molecular Display, 

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    Event date: 2004.7

    Language:English   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

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Research Project for Joint Research, Competitive Funding, etc. 5

  1. 免疫学的食中毒菌迅速検出法の開発

    2010.7

    出資金による受託研究 

  2. 酵素リアクターにおけるバイオマス分解酵素の進化分子工学による高機能化研究開発

    2008.4 - 2011.3

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    Grant type:Competitive

    申請者らが独自に開発したビーズディスプレイ法等を用いてセルラーゼの高機能化を目指す。

  3. 進化分子工学によるバイオマス糖化酵素の高機能化

    2006.6 - 2008.3

    企業からの受託研究 

  4. 遺伝子工学的手法を用いた酵素の機能改変

    2005.4 - 2006.3

    企業からの受託研究 

  5. 免疫反応検出用マイクロディバイスの研究開発

    2004.4 - 2005.3

    企業からの受託研究 

KAKENHI (Grants-in-Aid for Scientific Research) 21

  1. 革新的機能的ヒト抗体配列ーエピトープのハイスループット解析技術開発

    Grant number:22K18919  2022.6 - 2025.3

    科学研究費助成事業  挑戦的研究(萌芽)

    中野 秀雄, ダムナニョヴィッチ ヤスミナ, 兒島 孝明

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    Authorship:Principal investigator 

    Grant amount:\6500000 ( Direct Cost: \5000000 、 Indirect Cost:\1500000 )

    ヒト体内に存在するB細胞が産生する抗体分子は、感染防除や予防、がん細胞除去な どを担う一方、自己免疫疾患やがん細胞増殖にも関わるなど、多面的な機能を有することが知られている。抗体の標的分子は可変領域の配列で決まっているのであるが、その配列と標的であるエピトープを網羅的に解析する手法は存在せず、それらの関係性は不明のままである。本研究では、エマルジョン中での極微細反応場や無細胞タンパク質合成系、さらには次世代シーケンスやバイオインフォマティクスを駆使することで、抗体配列とエピトープを迅速安価に解析する新たな方法論を開発する。

  2. 腫瘍微小環境内 B 細胞を用いた転移性脳腫瘍に対する CAR-T 細胞療法の開発

    Grant number:22K09223  2022.4 - 2025.3

    科学研究費助成事業  基盤研究(C)

    大野 真佐輔, 中野 秀雄, 藤田 貢, 山下 公大

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    Authorship:Coinvestigator(s) 

    本研究計画は大きく①転移性脳腫瘍内のTLSおよびTABの検出②新鮮腫瘍組織からのTABのソーティングと抗体情報の抽出③CARの構築と抗腫瘍効果の検証の3つの段階に分かれる。大量の転移性脳腫瘍検体を用いての検索を必要とするため深層学習アルゴリズムを用いたハイスループット画像解析手法を用いてTLSの検出を行い、かつ抗体抽出に最適なTABの選定のための抗体コンビネーションを選定する。続いてEcobody法を用いた新鮮腫瘍組織からのTABの抽出と抗体情報の取得を行う。最後に入手した抗体の情報をもとにCARを構築し、in vitro、動物脳腫瘍モデルを用いた新規CARレパートリーの有効性を検証する

  3. Development and application of an early detection method for postoperative recurrence of colorectal cancer using tears

    Grant number:21K08778  2021.4 - 2024.3

    Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

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    Authorship:Coinvestigator(s) 

  4. Elucidation of the mechanism of maturation of tertiary lymph structure in gastric cancer tumor immune microenvironment and identification of the therapeutic antibodies

    Grant number:20H03752  2020.4 - 2023.3

    Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

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    Authorship:Coinvestigator(s) 

  5. Development of novel monoclonal antibody creation system using AI and single cell technoogy

    Grant number:19H02523  2019.4 - 2023.3

    Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

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    Authorship:Principal investigator 

    Grant amount:\17680000 ( Direct Cost: \13600000 、 Indirect Cost:\4080000 )

  6. Development of orthgonal liposomal fusion method using artificial phospholipids and its application

    Grant number:19K05160  2019.4 - 2022.3

    Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

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    Authorship:Coinvestigator(s) 

  7. Simultaneous production of monoclonal antibodies against multiple membrane proteins and their evaluations for the applications to therapeutic medicines

    Grant number:17H03468  2017.4 - 2021.3

    Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

    Tomita Masahiro

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    Authorship:Coinvestigator(s) 

    (1) Based on stereospecific targeting (SST) technique, we succeeded in producing conformation-specific monoclonal antibodies against membranous proteins with high efficiency, regardless of that it has been known to be quite difficult for generating them. (2) EGFR-recognizing and Swine Influenza Virus-binding rabbit monoclonal antibodies were obtained by using the Ecobody technology, directly from single B cells. In addition, a sand witch detection system for the virus was also constructed.(3) The formation of pairs of the different types of cells and the electrofusion of pairs were achieved by using the novel device with microband electrodes based on dielectrophoresis. (4) We developed the method for preparation of membrane protein-displaying artificial lipid membranes supported on spherical silica microbeads using direct membrane fusion with recombinant baculovirus virions expressing GPCRs like adrenergic receptors.

  8. 近赤外光を用いた新規迅速免疫測定法の開発

    2014.4 - 2016.3

    科学研究費補助金  挑戦的萌芽研究、課題番号:26630425

    中野 秀雄

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    Authorship:Principal investigator 

  9. 無細胞系ナノ構造化生物機能システムの開発

    2014.4 - 2016.3

    科学研究費補助金  特定領域研究

    中野 秀雄

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    Authorship:Principal investigator 

  10. 無細胞系ギガスクリーニング法の開発と応用

    2011.4 - 2015.3

    科学研究費補助金  基盤研究(B)

    中野 秀雄

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    Authorship:Principal investigator 

  11. 汎用的シグナルペプチド配列最適化法の開発

    2011.4 - 2013.3

    科学研究費補助金  挑戦的萌芽研究、課題番号:23656522

    中野 秀雄

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    Authorship:Principal investigator 

  12. 免疫細胞ネットワークのデジタル解析

    2007.4 - 2010.3

    科学研究費補助金  特定領域研究(公募,A03),課題番号:19021020

    中野 秀雄

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    Authorship:Principal investigator 

  13. 無細胞系分子ディスプレイシステムの構築と応用

    2007.3 - 2011.3

    科学研究費補助金  基盤研究(B)(一般),課題番号:19360373

    中野 秀雄

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    Authorship:Principal investigator 

  14. ヒトモノクローナル抗体のハイスループット取得法の開発

    2007.3 - 2009.3

    科学研究費補助金  萌芽研究,課題番号:19656218

    中野 秀雄

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    Authorship:Principal investigator 

  15. 酵素の光学選択性に関与する基質認識部位の網羅的解析

    2004.7 - 2007.3

    科学研究費補助金  基盤研究(B),課題番号:16360411

    中野秀雄

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    Authorship:Principal investigator 

  16. 細胞1個の遺伝情報とリンクしたプロテインアレイシステムの構築

    2004.7 - 2005.3

    科学研究費補助金  萌芽研究,課題番号:16656256

    中野秀雄

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    Authorship:Principal investigator 

  17. マイクロリアクタアレイによる超高密度タンパク質・ペプチド分子ライブラリの構築

    2000.7 - 2002.1

    科学研究費補助金  基盤研究(B)(1)(一般)

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    Authorship:Principal investigator 

    科研費

  18. ハイスループットモノクローナル抗体取得法に関する研究

    2000.7 - 2001.1

    科学研究費補助金  萌芽的研究

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    Authorship:Principal investigator 

    科研費

  19. バイオ研究支援機器としての無細胞遺伝子翻訳装置の開発

    1998.7 - 2002.1

    科学研究費補助金  基盤研究(B)(2)(展開)

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    Authorship:Coinvestigator(s) 

    科研費

  20. 無細胞分子進化システムの構築

    1997.7 - 1999.1

    科学研究費補助金  基盤研究(C)(2)(一般)

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    Authorship:Principal investigator 

    科研費

  21. 無細胞蛋白質合成系の効率化に関する研究

    1995.1 - 1997.12

    科学研究費補助金  一般研究(B)

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    Authorship:Coinvestigator(s) 

    科研費

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Industrial property rights 18

  1. タグ付抗体

    中野秀雄 兒島孝明 加藤晃代

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    Applicant:名古屋大学

    Application no:特願2014‒122773  Date applied:2014.6

    Announcement no:特開2016‑‒002009 

    Country of applicant:Domestic  

  2. 特定菌検出⽅方法

    加藤晃代 渕真吾 中野秀雄 田村廣人 三宅司郎

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    Applicant:名古屋大学等

    Application no:特願2013‑‒103740  Date applied:2013.5

    Announcement no:特開2014‑‒223033 

    Country of applicant:Domestic  

  3. 分⼦子ディスプレイ法及びその⽤用途

    中野秀雄 古澤聖司

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    Applicant:名古屋大学

    Application no:特願2008‑‒018324  Date applied:2008.1

    Announcement no:特開2009‑‒178067 

    Country of applicant:Domestic  

  4. エマルジョンを利用した核酸増幅法および核酸増幅キット、

    中野秀雄、武井義明、児島孝明

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    Application no:特願2005-2980  Date applied:2005

    Country of applicant:Domestic  

  5. 検査対象受体、検査装置、及び検査方法

    中野秀雄 吉村千里 石鹿孝典

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    Applicant:ブラザー工業株式会社

    Application no:特許出願2004-66206  Date applied:2004

    Announcement no:特許公開2005-257337 

    Country of applicant:Domestic  

  6. リガンド親和性複合蛋白質の取得方法、抗体スクリーニング方法、抗体スクリーニング用キット、医療方法、抗体医薬、非免疫反応性抗体医薬、投薬方法

    中野秀雄 姜 秀ピン

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    Applicant:名古屋大学

    Application no:特願2004-349309  Date applied:2004

    Country of applicant:Domestic  

  7. タンパク質の生産方法、タンパク質のスクリーニング方法、及びタンパク質の機能検索方法

    今村 千絵,高橋 治雄,中野 秀雄,山根 恒夫

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    Applicant:株式会社豊田中央研究所

    Application no:特許出願2002-219146  Date applied:2002.7

    Announcement no:特許公開2003-116590 

    Country of applicant:Domestic  

  8. Method for amplifying nucleic acid molecules and method for synthesizing proteins,"

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    Application no:USA Patent No. US 6,207,378 B1  Date applied:2000

    Country of applicant:Domestic  

  9. タンパク質の合成方法

    山根 恒夫, 中野 秀雄,河原崎 泰昌,関口 哲

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    Applicant:日本製粉株式会社

    Application no:特許出願平11-28142  Date applied:1999

    Announcement no:特許公開2000-224990 

    Country of applicant:Domestic  

  10. タンパク質の合成方法

    関口 哲,新畑 智也,布藤 聡,中野 秀雄,山根 恒夫

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    Applicant:日本製粉株式会社

    Application no:特許出願平11-323141  Date applied:1999

    Announcement no:特許公開2001-136971 

    Country of applicant:Domestic  

  11. 核酸分子の増幅方法

    山根 恒夫,中野 秀雄,関口 哲

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    Applicant:日本製粉株式会社

    Application no:特許出願平11-190800  Date applied:1999

    Announcement no:特許公開2001-17179 

    Country of applicant:Domestic  

  12. 核酸分子の増幅方法及びタンパクの合成方法

    山根 恒夫, 中野 秀雄,大内 将司, 奥村 れい子, 関口 哲

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    Applicant:日本製粉株式会社

    Application no:特許出願平10-81341  Date applied:1998

    Announcement no:特許公開平11-178582 

    Country of applicant:Domestic  

  13. タンパクの製造方法

    山根 恒夫, 中野 秀雄, 奥村 れい子,

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    Applicant:日本製粉株式会社

    Application no:特許出願平9-222371  Date applied:1997

    Announcement no:特許公開平11-56363 

    Country of applicant:Domestic  

  14. 無細胞タンパク合成系によるタンパクの合成方法及び装置

    山根恒夫、中野秀雄、田中忠明、関口哲

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    Applicant:日本製粉株式会社

    Application no:特許出願平9-90685  Date applied:1997

    Announcement no:特開平10-80295 

    Country of applicant:Domestic  

  15. 6-ヒドロキシニコチン酸モノオキシゲナーゼ遺伝子

    中野 秀雄, 川合 隆博,山根 恒夫, 長澤 透

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    Applicant:株式会社コスモ総合研究所 ,コスモ石油株式会社

    Application no:特許出願平7-287441  Date applied:1995

    Announcement no:特許公開平9-121864 

    Country of applicant:Domestic  

  16. 無細胞タンパク合成系を用いたタンパクの製造法

    関口 哲,船山 綾子,久保田 肇,山根 恒夫,中野 秀雄

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    Applicant:日本製粉株式会社

    Application no:特許出願平5-143242  Date applied:1993

    Announcement no:特開平7-194 

    Country of applicant:Domestic  

  17. 無細胞タンパク合成系を用いたタンパクの製造法

    山根恒夫、中野秀雄

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    Applicant:日本製粉株式会社

    Application no:特許出願平5-306194  Date applied:1993

    Announcement no:特開平6-225783 

    Country of applicant:Domestic  

  18. DNA増幅方法及びDNA増幅装置

    中野 秀雄, 山根 恒夫,長棟 輝行,養王田 正文遠藤 勲

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    Applicant:理化学研究所

    Application no:特許出願平4-185118  Date applied:1992

    Announcement no:特許公開平6-30776 

    Country of applicant:Domestic  

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Teaching Experience (On-campus) 6

  1. 生物反応工学

    2022

     詳細を見る

    微生物および酵素を用いたバイオプロセスの概要と基本理念について解説する

  2. Biomolecular Engineering

    2022

  3. 応用生命科学科学生実験

    2022

  4. 応用生命科学科学生実験

    2021

  5. Fundamentals of Chemistry II

    2011

  6. First Year Seminar B

    2011

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Teaching Experience (Off-campus) 5

  1. 合成・生物化学特論A

    2022.1 Kyoto University)

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    Level:Postgraduate 

  2. 無細胞タンパク質合成系の高機能化と応用

    2004.4 - 2005.3 静岡大学)

  3. 微生物工学

    1996.4 - 1997.3 国際工学院専門学校)

  4. 生物と工学

    2018.9 Chubu University)

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    Country:Japan

  5. バイオテクノロジー

    2009.4 - 2010.3 中部大学)