Updated on 2021/10/06

写真a

 
HITOMI, Kiyotaka
 
Organization
Graduate School of Pharmaceutical Sciences Department of Basic Medicinal Sciences Bioscience Professor
Cellular and Structural Physiology Institute Professor
Graduate School
Graduate School of Pharmaceutical Sciences
Undergraduate School
School of Agricultural Sciences
Title
Professor
Contact information
メールアドレス

Degree 1

  1. 農学博士 ( 1989.3   京都大学 ) 

Research Interests 7

  1. calcium

  2. transglutaminase

  3. Protein cross-linking

  4. 血液凝固

  5. メダカ

  6. keratinocyte

  7. 線維症

Research Areas 3

  1. Others / Others  / Applied Biochemistry

  2. Others / Others  / Applied Molecular Cell Biology

  3. Others / Others  / Functional Biochemistry

Current Research Project and SDGs 5

  1. characterization and appilcation of preferable substrate sequence of protein cross-linking enzyme, transglutaminase

  2. 表皮分化に空気暴露が必要とされる機構の解明と分化促進因子の解析

  3. Activating mechanism of epidermal transglutaminase by limite proteolysis

  4. Characterization of transglutaminase of slime mold upon cellualr injury

  5. fibrosis and transglutaminase, toward drug dicovery

Research History 12

  1. 名古屋大学大学院創薬科学研究科・教授(研究科長)

    2013.4 - 2017.3

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    Country:Japan

  2. Nagoya University   Graduate School of Pharmaceutical Sciences Department of Basic Medicinal Sciences Bioscience

    2012.4

  3. 名古屋大学大学院創薬科学研究科・教授(副研究科長)

    2012.4 - 2013.3

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    Country:Japan

  4. Nagoya University   Graduate School of Pharmaceutical Sciences Department of Basic Medicinal Sciences Bioscience

    2010.4

  5. 名古屋大学大学院生命農学研究科 准教授

    2007.4

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    Country:Japan

  6. ワシントン大学歯学部客員研究員(文部科学省在外研究員)

    2002.7 - 2002.8

  7. ワシントン大学歯学部客員研究員

    2001.6 - 2001.7

  8. 名古屋大学大学院生命農学研究科 助教授

    1999.4

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    Country:Japan

  9. 名古屋大学農学部応用生物科学科 助教授

    1995.11 - 1999.3

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    Country:Japan

  10. 名古屋大学農学部食品工業化学科 助手

    1989.10 - 1995.11

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    Country:Japan

  11. 京都大学教養部非常勤講師

    1989.4 - 1989.9

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    Country:Japan

  12. 日本学術振興会特別研究員

    1988.4 - 1989.9

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    Country:Japan

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Education 2

  1. Kyoto University   Graduate School, Division of Agriculture   Food Science and Chemistry

    1986.4 - 1989.3

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    Country: Japan

  2. Kyoto University   Faculty of Agriculture   Dept. of Food Science and Chemistry

    - 1984

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    Country: Japan

Professional Memberships 7

  1. 日本農芸化学会   理事 中部支部支部長

    2019.6 - 2021.5

  2. 日本生化学会   第95回大会運営幹事

    2016.9

  3. 日本動物細胞工学会

  4. 日本薬学会

    2016.1

  5. 日本免疫学会

    2021.7

  6. Japan Society for Cell Biology

  7. 日本分子生物学会

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Committee Memberships 3

  1. 日本農芸化学会   理事 中部支部支部長  

    2019.6   

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    Committee type:Academic society

  2. 日本生化学会中部支部   例会長(副支部長)  

    2017.8 - 2019.8   

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    Committee type:Academic society

    2019年5月25日 名古屋大学において、中部支部例会第83回例会を主宰した。

  3. トランスグルタミナーゼ研究会   代表世話人  

    2017.12   

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    Committee type:Other

Awards 3

  1. 農芸化学奨励賞

    2002.3   日本農芸化学会  

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    Country:Japan

  2. 天野エンザイム応用酵素シンポジウム奨励賞

    2003.6   天野エンザイム  

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    Country:Japan

  3. 天野エンザイム応用酵素シンポジウム奨励賞

    2010.6   天野エンザイム株式会社  

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    Country:Japan

 

Papers 113

  1. Investigation of mouse amniotic fluid for stimulating ability of keratinocyte differentiation depending on the fetal stage. Invited Reviewed

    Archives Biochemistry Biophysics   Vol. 711   page: 1   2021.10

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    Authorship:Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.abb.2021.109003.

  2. Spatially Resolved Identification of Transglutaminase Substrates by Proteomics in Pulmonary Fibrosis Invited Reviewed

    American Journal of Respiratory Cell Molecular Biology   Vol. 65   page: 319 - 330   2021.9

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1165/rcmb.2021-0012OC

  3. Role of Transglutaminase 2 in Cell Death, Survival, and Fibrosis Invited Reviewed

    Cells   Vol. 10 ( 7 ) page: 1842   2021.7

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.3390/cells10071842

  4. Thrombin-deficient mutant of medaka, a model fish, displays serious retardation in blood coagulation Reviewed

    Watanabe Y., Oguri R., Suzuki R., Ishikawa Y., Tatsukawa H., Hashimoto H., Hitomi K.

    Bioscience Biotechnology Biochemistry   Vol. 85 ( 4 ) page: 824 - 833   2021.4

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    Authorship:Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1093/bbb/zbaa098

  5. Identification and characterization of substrates crosslinked by transglutaminases in liver and kidney fibrosis Reviewed

    Tatsukawa Hideki, Takeuchi Taishu, Shinoda Yoshiki, Hitomi Kiyotaka

    ANALYTICAL BIOCHEMISTRY   Vol. 604   page: 113629   2020.9

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.ab.2020.113629

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  6. Transglutaminase orthologues in medaka fish - biochemical characterization and establishment of gene-deficient mutants Reviewed

    Meng Qi, Watanabe Yuko, Suzuki Risa, Oguri Rina, Tatsukawa Hideki, Hitomi Kiyotaka

    ANALYTICAL BIOCHEMISTRY   Vol. 604   page: 113610   2020.9

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.ab.2020.113610

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  7. Analysis of the expression of transglutaminases in the reconstructed human epidermis using a three-dimensional cell culture Reviewed

    Teshima Hirofumi, Kato Manami, Tatsukawa Hideki, Hitomi Kiyotaka

    ANALYTICAL BIOCHEMISTRY   Vol. 603   page: 113606   2020.8

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    DOI: 10.1016/j.ab.2020.113606

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  8. Gene disruption of medaka (Oryzias latipes) orthologue for mammalian tissue-type transglutaminase (TG2) causes movement retardation. Reviewed

    Watanabe Y., Okuya K., Takada Y., Kinoshita M., Yokoi S., Chisada S, Kamei Y., Tatsukawa H., Yamamoto N., Abe H., Hashimoto H., Hitomi K.

    Journal of biochemistry   Vol. 168 ( 3 ) page: 213 - 222   2020.4

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    Authorship:Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1093/jb/mvaa038

  9. Studies on differentiation-dependent expression and activity of distinct transglutaminases by specific substrate peptides using three-dimensional reconstituted epidermis.

    Tanabe Y, Yamane M, Kato M, Teshima H, Kuribayashi M, Tatsukawa H, Takama H, Akiyama M, Hitomi K

    The FEBS journal   Vol. 286 ( 13 ) page: 2536-2548   2019.7

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1111/febs.14832

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  10. Detection and identification of potential transglutaminase 2 substrates in the mouse renal glomeruli

    Ito Yoshimasa, Tatsukawa Hideki, Yamaguchi Hisateru, Takahashi Kazuo, Hitomi Kiyotaka, Yuzawa Yukio

    ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS   Vol. 660   page: 11-19   2018.12

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.abb.2018.10.001

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  11. ISOZYME-SPECIFIC IDENTIFICATION AND CHARACTERIZATION OF SUBSTRATES CROSSLINKED BY TRANSGLUTAMINASES IN LIVER FIBROSIS

    Tatsukawa H., Nakagawa H., Hitomi K.

    ALCOHOLISM-CLINICAL AND EXPERIMENTAL RESEARCH   Vol. 42   page: 33A-33A   2018.8

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  12. Higher susceptibility to osmolality of the medaka (Oryzias latipes) mutants in orthologue genes of mammalian skin transglutaminases. Reviewed

    Watanabe Y., Furukawa E. Tatsukawa H., Hashimoto H., Kamei Y., Taniguchi Y., Hitomi K.,

    Biosci. Biotechnol. Biochem   Vol. 82 ( 7 ) page: 1165-1168   2018.7

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  13. A granular parakeratosis animal model created by topical aluminum showed characteristic keratinocyte differentiation abnormalities including abnormal profilaggrin processing and cornified cell envelop formation

    Fujii M., Kishibe M., Ueda J., Hitomi K., Ishida-Yamamoto A.

    JOURNAL OF INVESTIGATIVE DERMATOLOGY   Vol. 138 ( 5 ) page: S68-S68   2018.5

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  14. Isozyme-specific comprehensive characterization of transglutaminase-crosslinked substrates in kidney fibrosis. Reviewed

    Tatsukawa H, Otsu R, Tani Y, Wakita R, Hitomi K.

    Sci Rep   Vol. 9 ( 8 ) page: 7306   2018.5

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  15. Biochemical characterization of medaka (Oryzias latipes) orthologue for mammalian Factor XIII and establishment of gene-edited mutant. Reviewed

    Horimizu R, Ogawa R, Y Watanabe, Tatsukawa H, Kinoshita M, Hashimoto H, and Hitomi K.

      Vol. 284   page: 2843-2855   2017.9

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  16. Global identification and analysis of isozyme-specific possible substrates crosslinked by transglutaminases using their substrate peptides in mouse liver fibrosis. Reviewed

    Tatsukawa H., Tani Y., Otsu R., Nakagawa H., Hitomi K.

      Vol. 7   page: 45049   2017.3

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  17. Characterization of medaka orthologue of human transglutaminase 2 (OlTGT). Reviewed

    Takada Y., Watanabe Y., Okuya K., Tatsukawa H., Hashimoto H, Hitomi K

    Biosci. Biotechnol. Biochem.   Vol. 81 ( 3 ) page: 469-474   2017.3

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  18. FRET-based detection of isozyme-specific activities of transglutaminases

    Tatsukawa H. Liu HH., Ooba S., Kamiya N., Nakanishi Y., Hitomi K.

    Amino Acids   Vol. 49   page: 615-623   2017.5

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  19. Analysis on transglutaminase 1 and its substrates using specific substrate peptide in cultured keratinocytes. Reviewed

    Yamane M., Sugimura K., Kawasaki H., Tatsukawa H., Hitomi K.

    Biochemical Biophysical Research Comunications   Vol. 478   page: 343-348   2016.9

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    DOI: 10.1016/j.bbrc.2016.07.051

  20. Biochemical characterization of medaka (Oryzias latipes) transglutaminases, OlTGK1 and OlTGK2, as orthologues of human keratinocyte-type transglutaminase.

    A Kikuta, E Furukawa, R Ogawa, N Suganuma, M Saitoh,T Nishimaki, T Katsumura, Hi Oota, T Kawamoto, H Tatsukawa H Hashimoto and K Hitomi

    PLOS One     2015.12

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    DOI: DOI: 10.1371/journal.pone.0144194

  21. Distribution of transglutaminase family members in mouse tissues and whole body sections.

    Tatsukawa H., Abe N., Ohashi S., and Hitomi K.

    Biochem Biphys Res Commun.   Vol. 467   page: 10146-1051   2015.11

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  22. Transglutaminase activity is decreased in large arteries from hypertensive rats, compared to normotensive controls. Reviewed

    Petersen-Jones HG, Johnson KB, Hitomi K, Tykocki NR, Thompson JM, Watts SW.

    Am J Physiol Heart Circ Physiol.   Vol. 308   page: 592-602   2015.3

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  23. Early response as shown by enhancement of transglutaminase 1 expression after cisplatin-induced acute kidney injury. Reviewed

    Furukawa K., Yamane M., Tatsukawa H., Hitomi K.

    Arch Biochem Biophys.   Vol. 586   page: 27-32   2015.9

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  24. Transglutaminase activity is decreased in large arteries from hypertensive rats compared with normotensive controls. Reviewed

    Petersen-Jones HG, Johnson KB, Hitomi K, Tykocki NR, Thompson JM, Watts SW.

    Am J Physiol Heart Circ Physiol.   Vol. 308   page: 592-602   2015.3

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  25. Tissue Transglutaminase Mediated Tumor-Stroma Interaction Promotes Pancreatic Cancer Progression. Reviewed

    Lee J, Condello S., Yakubov B., Emerson R., Caperell-Grant A., Hitomi K., Xie J., Matei D.

    Clin Cancer Res.   Vol. 21   page: 4482-4493   2015.8

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  26. Lamellar ichthyosis with pseudoexon activation in the transglutaminase 1 gene Reviewed

    Suga Y., Tsuda T., Nagai M., Sakaguchi Y., Jitsukawa O., Yamamoto M., Hitomi K., Yamanishi K.

    J. Dermatology   Vol. 42   page: 642-645   2015

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  27. Hypohidrosis plays a crucial role in the vicious circle of bathing suit ichthyosis: a case with summer exacerbation Reviewed

    Washio K, Fukunaga A, Terai M, Hitomi K, Yamanishi K, Nishigori C

    Acta Dermato-Venerelogica   Vol. 94   page: 349-350   2014.5

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  28. Transglutaminase activity arising from Factor XIIIA is required for stabilization and conversion of plasma fibronectin into matrix in osteoblast cultures. Reviewed

    C. Cui, Wang S., Myneni VD, Hitomi K., Kaartinen M.

    Bone   Vol. 59   page: 127-138   2014.2

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  29. Identification of a highly reactive substrate peptudei for transglutaminase 6 and its use in detecting TGase activity in the skin epidermis Reviewed

    Fukui M., Kuramoto K., Yamasaki R., Shimizu Y., Itoh M., Kawamoto T., Hitomi K.

      Vol. 280   page: 1420-1429   2013.1

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  30. Phage-displayed peptide liberary screening for preferred human substrate peptide sequences for transglutaminase 7 Reviewed

    Kuramoto K., Yamasaki R., Shimizu Y., Tatsukawa H., Hitomi K

    Archives Biochemistry and Biochemistry   Vol. 537 ( 1 ) page: 138-143   2013

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    DOI: 0.1016/j.abb.2013.07.010

  31. Variations in both TG1 and TG2 isozyme-specific in situ activities and protein expressions during mouse embryonic development. Reviewed

    Itoh M., Tatsukawa H., Lee E-S, Yamanishi K., Kojima S., and Hitomi K.

    J. Histochem. Cytochem.   Vol. 61   page: 793-801   2013.11

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    DOI: 10.1369/0022155413501676

  32. Spatial arrangement of proteins using scCro-tag: application for an in situ enzymatic microbead assay

    Kojima Takaaki, Hata Jumpei, Oka Hiroya, Hayashi Kenta, Hitomi Kiyotaka, Nakano Hideo

    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY   Vol. 82 ( 11 ) page: 1911-1921   2018.11

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    DOI: 10.1080/09168451.2018.1501265

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  33. Targeting transglutaminase 2 partially restores extracellular matrix structure but not alveolar architecture in experimental bronchopulmonary dysplasia

    Mizikova Ivana, Pfeffer Tilman, Nardiello Claudio, Solaligue David E. Surate, Steenbock Heiko, Tatsukawa Hideki, Silva Diogo M., Vadasz Istvan, Herold Susanne, Pease Richard J., Iismaa Siiri E., Hitomi Kiyotaka, Seeger Werner, Brinckmann Juergen, Morty Rory E.

    FEBS JOURNAL   Vol. 285 ( 16 ) page: 3056-3076   2018.8

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1111/febs.14596

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  34. Detyrosinated Glu-tubulin is a substrate for cellular Factor XIIIA transglutaminase in differentiating osteoblast. Reviewed

    Wang, S., Cui C., Hitomi K., Kaartinen M.

    Amino Acids   Vol. 46   page: 1513-1526   2014

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  35. Factor XIII-A transglutaminase acts as a switch between preadipocyte proliferation and differentiation. Reviewed

    Myneni VD, Hitomi K., Kaartinen M.

    Blood   Vol. 124   page: 1344-1353   2014

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  36. Distinct protein expression and activity of transglutaminases found in different epidermal tumors. Reviewed

    Karashima T., Furumura M., Ishii N., Ohyama B., Saruta H., Natsuaki Y., Ohata C., Tsuruta D., Hitomi K., and Hashimoto T.

    Exp. Dermatol.   Vol. 23   page: 433-435   2014

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  37. タンパク質架橋化酵素の高反応性基質の探索と活用 Invited

    人見清隆

    バイオサイエンスとバイオインダストリー   Vol. 70 ( 6 ) page: 442-447   2012.11

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    Language:Japanese  

  38. A distinct effect of transient and sustained upregulation of cellular factor XIII in the goldfish and optic nerve on optic nerve regeneration Reviewed

    Sugitani, K., Ogai K., Hitomi, K., Nakamura-Yonehara K., Shintani T., Noda M., Kato S.

    Neurochem. Int.   Vol. 61   page: 423   2012.8

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  39. Vena cava and aortic smooth muscle cells express transglutaminase 1 and 4 in addition to tranglutaminase 2 Reviewed

    Johnson KB, Hitomi K, Itoh M, Bakker EN, , Watts SW et al.

    American Journal of Physiology (circulation physiology)   Vol. 302 ( 7 ) page: H1355-1366   2012.4

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    DOI: 10.1152/ajpheart.00918.2011

  40. Knocking-in the R142C mutation in transglutaminase 1 disrupts the stratum corneum barrier and postnatal survival of mice Reviewed

    Nakagawa N., Yamamoto M., Imai Y., Sakaguchi Y., Takizawa T., Ohta N., Yagi N., Hatta I., Hitomi K., Takizawa T., Takeda J., Tsuda T., Matsuki M. Yamanishi K.

    Journal of Dermatological Sciences   Vol. 65 ( 3 ) page: 196-206   2012.3

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    DOI: 10.1016/j.jdermsci.2011.12.011

  41. Bathing suit ichthyosis with summer exacerbation: a temperature-sensitive case Reviewed

    Yamamoto M., Sakaguchi Y., Itoh M., Nakagawa N., Fukunaga A., Hitomi K., Yamanishi K.

    Biritish Journal of Dermatology   Vol. 166 ( 3 ) page: 671-693   2012.3

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    DOI: 10.1111/j.1365-2133.2011.10594.x

  42. Hornerin is a component of the epidermal cornified cell envelopes Reviewed

    Henry J, Hsu C-Y, Haftek, M., Nachat R.,de Koning HD., Gardina-Galera I, Hitomi K. Balica, S., Jean-Decoster, C., Schmitt, A-M., Serre, G., SimonM

    FASEB Journal   ( 25 ) page: 1567-1576   2011.5

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    DOI: 1567-1576

  43. Screening of substrate peptide sequences for tissue-type transglutaminase (TGase 2) Using T7 Phage cDNA Library. Reviewed

    Sugimura Y., Yamashita H, Hitomi K.

    Cytotechnology   ( 63 ) page: 111-118   2011.3

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    DOI: 111-118

  44. In situ detection of active transglutaminases for keratinocyte-type (TGase 1) and tissue-type (TGase 2) using fluorescence-labeled highly reactive substrate peptides. Reviewed

    Itoh M., Kawamoto T., Tatsukawa H., Kojima S., Yamanishi, K., Hitomi, K.

    Journal of Histochemistry and Cytochemistry   ( 59 ) page: 180-187   2011.2

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    DOI: 180-187

  45. Identification of a preferred substrate peptide for transglutaminase 3 and detection of in situ activity in skin and hair follicles Reviewed

    Yamane, A., Fukui, M., Sugimura Y., Itoh, M., Prez Alea P., Thomas V., El Alaoui S., Akiyama M., Hitomi K.

    FEBS Journal   Vol. 277   page: 3564–3574   2010.9

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  46. The cystatin M/E-cathepsin L balance is essential for tissue homeostasis in epidermis, hair follicles, and cornea. Reviewed

    Zeeuwen PM., van Vlijmen-Willems I., Cheng, T., Rodijk-Olthuis1, D., Hitomi K., Hara-Nishimura I., John S. Smyth, N., Reinheckel T., Hendriks WJ., and Schalkwijk J.

    FASEB J.   Vol. 24   page: 3744-3755   2010.5

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  47. Transglutaminase1 Preferred Substrate Peptide K5 Is an Efficient Tool in Diagnosis of Lamellar Ichthyosis. Reviewed

    Masashi Akiyama, Kaori Sakai, K., Teruki Yanagi, T., Fukushima, S., Ihn H., Hitomi K., and Hiroshi Shimizu, H.

    Am. J. Pathol.   Vol. 129   page: 2306-2309   2010.4

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  48. *A specific colorimetric assay for measuring teranglutamianse 1 and factor XIII activities. Reviewed

    Analytical Biochemistry   Vol. 394   page: 281-283   2009.11

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  49. A biochemical pathway that controls skin barrier formation: expression of its key components in inflammatory skin diseases and in reconstructed skin. Reviewed

    T. Cheng, G.S. Tjabringa, I.M.J.J. van Vlijmen-Willems, K. Hitomi*, P.E.J. van Erp, J.Schalkwijk, and P.L.J.M. Zeeuwen

    Br. J. Dermatol.   Vol. 161   page: 253-264   2009.8

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  50. Development of an isoenzyme-specific colorimetric assay for tissue transglutaminase 2 cross-linking activity Reviewed

    Perez Alea M., Kitamura, M., Martin, G., Thomas, V., Hitomi, K., El Alaoui,S.

    Anal. Biochem.   Vol. 389   page: 150-156   2009.6

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  51. Colocalization of cystatin M/E and its target proteases suggests a role in terminal differentiation of human hair follicle and nail Reviewed

    Cheng, T., van Vlijmen-Willems, IM, Hitomi, K., Schlkwijk, J. Zeeuwen, P.

    J. Invest. Dermatol.   Vol. 129 ( 5 ) page: 12132-1242   2009.5

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  52. *Identification of preferred substrate sequences for transglutaminase 1: development of a novel peptide that can efficiently detect cross-linking enzyme activity in the skin. Reviewed

    Sugimura Y, Hosono M, Kitamura M, Tsuda T, Yamanishi K, Maki M and Hitomi K.

    FEBS Journal   Vol. 275   page: 5667-5677   2008.11

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  53. Identification of the preferred substrate sequences of microbial transglutaminase from Streptomyces mobaraensis using a phage-displayed peptide library. Reviewed

    Sugimura Y., Yokoyama K., Nio N., Maki M., and Hitomi K.

    Arch. Biophys. Biochem.   Vol. 477   page: 379-383   2008.6

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  54. Transglutaminase 2 activity promotes membrane resealing after mechanical damage in the lung cancer cell line A549 Reviewed

    Kawai Y. Wada F., Sugimura Y., Maki M., and Hitomi K

    Cell Biol. Int.   Vol. 32   page: 928-934   2008.4

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  55. Gene expression signatures that classify the mode of invasion of primary oral squamous cell carcinomas. Reviewed

    Kondoh N, Ishikawa T, Ohkura S, Arai M, Hada A, Yamazaki Y, Kitagawa Y, Shindoh M, Takahashi M, Ando T, Sato Y, Izumo T, Hitomi K, Yamamoto M.

    Mol. Carcinogen.   Vol. 47   page: 744-756   2008.4

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  56. Human calpain 7/PalBH associates with a subset of ESCRT-III-related proteins in its N-terminal region and partly localizes to endocytic membrane compartments Reviewed

    Yorikawa C., Takaya E., Ohsako Y., Terasawa, Y., Hamakubo T., Mochizuki Y., Iwanari H., Kodama T., Maeda T., Hitomi K., Shibata H., Maki M.

    J. Biochem.   Vol. 143   page: 731-735   2008.3

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  57. Novel site-specific immobilization of a functional protein using a preferred substrate sequence for transglutaminase 2 Reviewed

    Y. Sugimura, H. Ueda, M. Maki, and K. Hitomi

    Journal of Biotechnology   Vol. 131   page: 121-127   2007.8

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  58. *Identification of substrates for mammalian-type transglutaminase in Physarum polycephalum, an acellular slime mold, upon cellular mechanical damage Reviewed

    Wada F., Hasegawa H., Hanai Y., Nakamura A., Sugimura Y. , Kawai Y., Sasaki N., Shibata H., Maki M. and Hitomi K

    FEBS Journal   Vol. 274 ( 11 ) page: 2766-2744   2007.5

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  59. A novel strategy for generation of monoclonal antibodies from single B cells using rt-PCR technique and in vitro expression Reviewed

    Jiang, X., Suzuki, H., Hanai, Y., Wada F., Hitomi, K., Yamane, T., Nakano, H

    Biotechnol. Prog.   Vol. 22   page: 979-988   2006.7

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  60. Cystatin M/E is a high affinity inhibitor of cathepsin V and cathepsin L by a reactive site that is distinct from the legumain-binding site Reviewed

    Cheng, T., Hitomi, K., Vlijmen-Willems, J. J., Jongh, G., J., Yamamoto K., Nihsi, K., Watts, C., Reinhecke, T., Schalkwijk, J. and P. Zeeuwen

    The Journal of Biological Chemistry   Vol. 281 ( 23 ) page: 15893-15899   2006.6

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  61. *Screening for the preferred substrate sequence of transglutaminase using a phage-displayed peptide library Reviewed

    Sugimura, Y., Hosono, M., Yoshimura, T., Maki, M., and Hitomi, K.

    The Journal of Biological Chemistry   Vol. 281 ( 26 ) page: 17699-17706   2006.6

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  62. Generation of monoclonal antibodies using simplified single-cell reverse transcription-polymerase chain reaction and cell-free protein synthesis. Reviewed

    Ali, M., Hitomi, K., and Nakano, H.

    J. Biosci. Bioeng.   Vol. 101   page: 284-286   2006.3

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  63. Reevaluation of the predicted gene structure of dictyostelium cystatin A3 (cpiC) by nucleotide sequence determination of its cDNA and its phylogenetic position in the systatin superfamily Reviewed

    El-halawany MS, Shibata, H., Hitomi, K., and Maki, M.

    Molecular Biology Reports   Vol. 32 ( 4 ) page: 257-264   2005.12

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  64. Transglutaminase in the skin epidermis Invited Reviewed

      Vol. 15   page: 313-319   2005.9

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  65. "Analyses of expression and localization of two mammalian-type transglutaminases in Physarum Polycephalum, an acellular slime mold." Reviewed

    "Wada, F., Ogawa, A., Hanai, Y., Nakamura, A. Maki, M., and Hitomi, K."

    J. Biochem.   Vol. 136   page: 665-672   2004.11

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  66. "DdAlix, an Alix/AIP1 homolog in Dictyostelium discoideum, is required for multicellular development under low Ca2+ conditions." Reviewed

    "Ohkouchi, S., Medhat, El-H., Maeda, M., Shibata, H., Hitomi, K., and Maki, M."

    Gene   Vol. 337   page: 131-139   2004.10

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  67. Evidence that unrestricted legumain activity is involved in disturbed epidermal cornifcation in cystatin M/E deficient mice. Reviewed

    "Zeeuwen, P.M., van Vlijmen-Willems, I., Olthuis1, D., Johansen, H.T., Hitomi, K., Hara-Nishimura, I., Powers, J. C., James, K. E., Camp, H. J., Lemmens1, R., and Schalkwijk1, J."

    Hum. Mol. Genet.   Vol. 13   page: 1069-1079   2004.10

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  68. Identification of cysteine protease inhibitors that belong to cystatin family 1 in the cellular slime mold Dictyostelium discoideum. Reviewed

    "Medhat, El-H., Ohkouti, S., Shibata, H., Hitomi, K., and Maki, M."

    Biol. Chem.   Vol. 385   page: 547-550   2004.6

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  69. Immunological detection of proteolytically activated epidermal-type transglutaminase (TGase 3) using cleavage site specific antibody Reviewed

    "Hitomi, K., Ikeda, N., and Maki, M."

    Biosci. Biotechnol. Biochem.   Vol. 67   page: 2492-2494   2003.11

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  70. Analysis of epidermal-type transglutaminase in human stratified epithelia and cultured keratinocytes using monospecific antibodies Reviewed

    "Hitomi, K., Nakayama, T., Presland, R.B., Fleckman, P., Dale, B. A., and Maki , M."

    J. Dermatol. Sci.   Vol. 32   page: 95-103   2003.7

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  71. Molecular Evolution of Transglutaminase: from bacteria to animal Invited

    "Hitomi, K."

    Recent Research Developmemts in Biophysics and Biochemistry   Vol. 3   page: 223-234   2003.1

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  72. Identification of Mammalian-type Transglutaminase in Physarum polycephalum: Evidences from cDNA sequence and involvement of GTP in the regulation of transamidating activity Reviewed

    "Wada, F., Nakamura, Masutani, T., A., Ikura, K., Maki, M., and Hitomi K."

    Eur. J. Biochem.   Vol. 269   page: 3451-3460   2002.9

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  73. Identification and Characterization of Two Penta-EF-Hand Ca(2+)-Binding Proteins in Dictyostelium discoideum Reviewed

    "Ohkouchi S., Nishio K., Maeda, M., Hitomi K., Adachi H., and Maki M."

    J. Biochem (Tokyo).   Vol. 130   page: 207-215   2001.8

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  74. Analysis of Epidermal Transglutaminase (TGase3) Expression in Mouse Tissues and Cell Lines Reviewed

    "Hitomi, K., Horio, Y., Ikura, K., Yamanishi, K., and Maki, M."

    Int. J. Biochem. & Cell Biol   Vol. 33   page: 491-498   2001.6

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  75. "Peflin and ALG-2, Members of the Penta-EF-Hand Protein Family, Form a Heterodimer that Dissociates in a Ca2+-Dependent Manner"

    "Kitaura, Y., Matumoto S., Satoh,H., Hitomi, K., and Maki, M."

    J. Biol. Chem   Vol. 276   page: 14503-14508   2001.4

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  76. Characterization of human recombinant transglutaminase 1 purified from baculovirus-infected insect cells Reviewed

    "Hitomi K., Ymagiwa, Y., Ikura, K., Yamanishi, K., and Maki, M."

    Biosci. Biochem. Biochem.   Vol. 64   page: 2128-2137   2000.10

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  77. Four types of calpastatin isoforms with distinct amino-terminal sequences are specified by alternative first exons and differentially expressed in mouse tissues

    "Takano, J., Watanabe, M., Hitomi, K., and Maki, M."

    Journal of Biochemistry   Vol. 128   page: 83-92   2000.6

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  78. "GTP, an Inhibitor of Transglutaminases, is Hydrolyzed by Tissue-type Transglutaminase(TGase 2)"

    "Hitomi, K., Ikura, K., and Maki, M."

    Biochem. Biosci. Biotechnol.   Vol. 64   page: 657-659   2000.4

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  79. "Peflin, a novel member of the five-EF-hand-protein family, is similar to the Apoptosis-linked-gene 2 (ALG-2) protein but possesses nonapeptide repeats in the N-terminal hydrophobic region."

    "Kitaura, Y., Sato, H., Watanabe, M., Kawai, T., Hitomi, K., and Maki, M."

    Biochem. Biophys. Res. Commun   Vol. 263   page: 68-75   1999.10

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  80. Purification of recombinant calpastatin in Escherichia coli

    "Maki, M. and Hitomi, K"

    Methods in Molecular Biology   Vol. 144   page: 85-94   1999.1

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  81. Preparation of calpastatin Samples for Western blotting

    "Maki, M. and Hitomi, K."

    Methods in Molecular Biology   Vol. 144   page: 95-98   1999.1

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  82. Characterization of recombinant mouse epidermal-type transglutaminase (TGase 3):Regulation of its activity by proteolysis and guanine nucleotides. Reviewed

    "Hitomi, K., Kanehiro, S., Ikura, K., and Maki, M."

    Journal of Biochemistry   Vol. 125   page: 1048-1054   1999.1

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  83. Structure of mouse calpastatin isoforms:Implication of species-common and species-specific alternative splicing Reviewed

    "Takano J., Kawamura T., Murase, M., Hitomi, K., and Maki, M."

    Biochemcal and Biophysical Research Communication   Vol. 260   page: 339-345   1999.1

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  84. Purification of recombinant calpastatin in Escherichia coli Invited

    Methods in Molecular Biology   Vol. 144   page: 85   1999

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  85. Preparation of calpastatin samples for Western blotting Invited

    Methods in Molecular Biology   Vol. 144   page: 95   1999

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  86. Regulation of matrix metalloproteinase-9 a inhibition of tumor invasion by the membrane-anchored glycoprotein RECK. Reviewed

    "Takahashi C., Sheng, Z., Horan, T. P., Kitayama, H., Maki, M., Hitomi, K., Kitaura, Y., Takai, S., Sasahara, R.M., Horimoto, R., Ikawa, Y., Ratzkin, B., Arakawa, T., and Noda, M.,"

    Proceedings of the National Academy of Sciences of the U.S.A.   Vol. 95   page: 13221-13226   1998.10

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  87. Expression of biologically active human calpastatin in baculovirus-infected insect cells a in Ecsherichia coli. Reviewed

    "Hitomi, K. , Yokoyama, A., and Maki, M.,"

    "Bioscience, Biotechnology, a Biochemistry"   Vol. 62   page: 136-141   1998.1

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  88. Purification a characterization of the active-site-mutated recombinant human μ-calpain in baculovirus-infected Insect cells.

    "Hitomi, K., Uchiyama, Y., Ohkubo, I., Kunimatsu, M., Sasaki, M., and Maki, M.,"

    Biochemcal a Biophysical Research Communication   Vol. 246   page: 681-685   1998.1

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  89. Ascorbate-depeent enhancement of nitric oxide formation in activated macrophages

    "Mizutani, A., Maki, H., Torii, Y., Hitomi, K., and Tsukagoshi, N.,"

    Nitric Oxide   Vol. 2   page: 235-241   1998.1

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  90. Calcium-iuced exposure of hydrophobic surface on mouse ALG-2 which is a member of the penta-EF-ha protein family.

    "Maki, M., Yamaguchi, K., Kitaura, Y., Sato, H., and Hitomi, K.,"

    Journal of Biochemistry (Tokyo)   Vol. 124   page: 1170-1177   1998.1

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  91. A growing family of the calcium-binding proteins with five EF-hand motifs

    "Maki, M., Narayam, A.V.L., and Hitomi, K"

    Biochem. J   Vol. 328   page: 718-720   1997.1

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  92. Ascorbate-dependent expression of ubiquitin genes in guinea pigs. Reviewed

    "Mizutani, A., Nagkagawa, N., Hitomi, K., and Tsukagoshi, N.,"

    International Journal of Biochemistry a Cell Biology   Vol. 29   page: 575-582   1997.1

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  93. Functional properties of recombinant calpain I a of mutants lacking domains III a IV of the catalytic subunit.

    "Vilei, E.M. , Calderara, S., Anagli, J., Berardi, S., Hitomi, K., Maki, M., and Carafoli, E.,"

    Journal of Biological Chemistry   Vol. 272   page: 25802-25808   1997.1

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  94. Functional properties of reconbinant calpain I and of mutants lacking domains III and IV of the catalyfic subunit(共著) Reviewed

    J. Biol. Chem.   Vol. 272   page: 25802   1997

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  95. Ascorbic acid:Role of Ascorbic Acid on Modulation of Gene Expression

    "Hitomi, K. and Tsukagoshi, N."

    Subcellular Biochemistry (Plenum Publishing Co.)   Vol. 25   page: 41-56   1996.1

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  96. Synergistic effect of BMP-2 a ascorbate on the phenotypic expression of osteoblastic MC3T3- E1. Reviewed

    "Torii Y., Hitomi K., and Tsukagoshi N.,"

    Molecular and Cellular Biochemistry   Vol. 165   page: 25-29   1996.1

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  97. Demonstration of alkaline phosphatase participation in the mineralization of osteoblasts by antisense RNA approach.

    "Torii Y., Hitomi K., Yamagishi Y., and Tsukagoshi N.,"

    Cell Biology International   Vol. 20   page: 459-464   1996.1

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  98. 4-hydroxy-2-nonenal cytotoxitciy in renal proximal tubular cells: Protein modification a redox alteration.

    "Fukuda, A., Osawa, T., Hitomi, K., and Uchida, K.,"

    Archives of Biochemistry a Biophysics   Vol. 333   page: 419-426   1996.1

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  99. cDNA sequence for rKST-1, a novel member of the sodium ior dependent glucose cotransporfer family Reviewed

    Biochimica et Biophysica Acta   Vol. 1190 ( 2 ) page: 469   1994

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  100. L-Ascorbic Acid 2-Phosphate promotes osteoblastic differentiation of MC3T3-E1 Mediated by Accumulation of Type I Collagen (共著)

    Journal of Nutritional Science and Vitaminology   Vol. 40   page: 229-238   1994

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  101. Ascorbate-dependent elevation of mRNA levels for cytochrome P450s induced by polychlorinated biphenyls

    Biochemical Pharmacology   Vol. 46 ( 1 ) page: 186   1993

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  102. Increase in the activity of Alkaline Phosphatase by L-Ascorbic Acid 2-phosphate in a Human Oskoblast Cell Line, HuO-3N1

    Journal of Nutritional Science and Uitaminology   Vol. 38   page: 535   1992

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  103. Recombinant Human Erythropoietir and Its Receptor

    Trends in Animal Cell Culture Technology     1990

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    Language:English  

  104. Establishment of an erythroid cell line (JK-1) that spontaneously differentiation to red cells(共著)

    Cancer   Vol. 66   page: 1544-1541   1990

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    Language:English  

  105. Expression of the Erythropoietin Receptor on a Human Myeloma Cell Line

    Biochemical Biophysical Research Communication     page: 170   1990

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  106. Solubilization and Characterization of Erythropoietin Receptor from Transplantable Mouse Erythroblastic Leukemic cell Line.

    Biochemical Biophysical Research Communication     page: 160   1989

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  107. Continuous Production of erythropoietin with Immobilized Animal Cells(共著)

    Applied Microbiology Biotechnology   Vol. 29   page: 544   1988

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  108. Stromal Cell-dependent Growth of Leukemic Cells from Murine Erythroblastic Leukemia(共著)

    Japanese Journal of Cancer Research   Vol. 79   page: 931   1988

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  109. Production of Erythropoietin by BHK Cells Growing on the Microcarriers Trapped in Alginate Cells(共著)

    Cytotechnology   Vol. 2   page: 141-145   1988

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  110. Erythropoietin Receptor of a Human leukemic Cell Line with Erythroid Characteristics

    Biochemical Biophysical Research Communication     page: 79   1988

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  111. 食品・医薬品分野における蛋白質テーラリング(共訳)

    学会出版センター     1988

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    Language:Japanese  

  112. Characterization of Erythropoietin Receptor of Murine Erythroid Cells.

    European Journal of Biochemistry     page: 168   1987

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    Language:English  

  113. Isolation and Characterization of cDNA Encoding Rabbit Reticulocyte 2,3-Bisphospho-glycerate synthase

    Gene     page: 44   1986

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    Language:English  

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Books 17

  1. 食品・バイオにおける最新の酵素利用

    手島裕文、加藤まなみ、辰川英樹、人見清隆( Role: Contributor)

     シーエムシー出版  2019 

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    Language:Japanese Book type:General book, introductory book for general audience

  2. 血液凝固のメカニズムと病態について魚類を用いて解析する

    鈴木里沙、Qi Meng, 渡辺優子、人見清隆( Role: Joint author)

    化学と生物  2018 

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    Language:Japanese Book type:Scholarly book

  3. 生命に内在するタンパク質接着酵素をメダカで研究する

    渡邊優子、人見清隆( Role: Joint author)

    生化学会誌  2018 

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    Language:Japanese

  4. 組織の線維化に伴い架橋される基質タンパク質群の網羅的解析・同定

    辰川英樹、人見清隆( Role: Joint author)

    日本応用酵素協会誌  2017 

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    Language:Japanese

  5. Preferred Substrate Structure of Transglutaminases

    Kiyotaka Hitomi, Hideki Tatsukawa( Role: Sole author)

    Springer  2016.3  ( ISBN:978-4-431-55823-1

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    Language:English

  6. Identification and application of the preferred substrate peptides for transglutaminases

    ( Role: Sole author)

    2009.8 

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    Language:Japanese

  7. *Preferred substrate sequences for transglutaminase 2: screening using a phage-displayed peptide library

    Hitomi, K., Kitamura, M. Sugimura, Y.( Role: Joint author)

    Amino Acids,  2009.4 

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    Language:English

  8. Animal Cell Technology 15:Characterization of highly reactive sequences for transglutaminase 2 and factor XIIIa

    Sugimura Y., Kitamura M., Hosono H., Shibata, H., Maki M. and Hitomi K( Role: Joint author)

    Kluwar Academic Publishers  2008 

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    Language:English

  9. Animal Cell Technology 14: Immunological Analysis of Epidermal-type Transglutaminase (TGase 3) in Epithelium.

    Kiyotaka Hitomi, Kanae Yamamoto, Koji Nishi, Yoshiaki Sugimura, and Masatoshi Maki( Role: Joint author)

    Kluwar Academic Publishers  2006 

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    Language:English

  10. トランスグルタミナーゼによる低分子量Gタンパク質の機能制御

    和田文孝、人見清隆( Role: Joint author)

    化学と生物  2005.10 

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    Language:Japanese

  11. Post translational modification of transglutaminase

    ( Role: Sole author)

    2005.6 

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    Language:Japanese

  12. トランスグルタミナーゼの分子進化と多様性

    人見清隆、和田文孝( Role: Joint author)

    化学と生物  2003.6 

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  13. 皮膚表皮に存在するカルシウム依存性タンパク質架橋化酵素の発現と活性制御機構に関する研究

    人見清隆( Role: Sole author)

    日本農芸化学会誌  2003.2 

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    Language:Japanese

  14. Recent Research Developmemts in Biophysics and Biochemistry: Molecular Evolution of Transglutaminase: from bacteria to animal

    Kiyotaka Hitomi( Role: Sole author)

    Research Signpost  2003 

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  15. Animal Cell Technology:Basic and Applied Aspects

    "Hitomi, K., Ikura, K., and Maki, M.( EDITOR:Shirahata, S.)"( Role: Joint author)

    Kluwer Academic Publishers  2002 

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    Characterization of recombinant transglutaminases 1 and 3 expressed in baculovirus system

  16. Animal Cell Technology:Expression and Characterization of Mouse Epidermal Transglutaminase in Baculovirusinfected Insect Cells. (共著)

    ( Role: Sole author)

    1999 

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  17. Animal Cell Technology: Expression and characerization of human calpain and calpastatin

    "Hitomi K. and Maki, M (EDITOR:Kitagawa Y.)"( Role: Joint author)

    1998 

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    Expression and Characterization of human calpain and calpastatin using baculovirus system

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Presentations 25

  1. 生命に内在するタンパク質架橋化酵素の生理機能と関連病態 Invited

    人見清隆

    名城大学 難治性疾患発症メカニズム研究センター第一回セミナー 

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    Event date: 2020.2

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  2. タンパク質架橋化酵素トランスグルタミナーゼの線維症に伴う活性変動と基質群の解析 Invited

    人見清隆

    日本腎臓学会学術総会 シンポジウム 線維化、リモデリング機序と新規治療 

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    Event date: 2019.6

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:日本腎臓学会   Country:Japan  

  3. ANALYSES OF TRANSGLUTAMINASES ACTIVITIES AND THEIR SUBSTRATES USING SPECIFIC SUBSTRATE PEPTIDES Invited International conference

    Hitomi K.

    Univ. of Debrecen (Hungary) Symposium 

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    Event date: 2017.8

    Language:English   Presentation type:Oral presentation (general)  

    Country:Hungary  

  4. Characterization of substrate peptide for skin TGs and applications for detection of activities and substrates International conference

    Gordon Research Conference in Italy Transglutaminases in Human Disease Processes 

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    Event date: 2014.6 - 2014.7

    Language:English   Presentation type:Oral presentation (invited, special)  

    Country:Italy  

  5. Identification and applications of the highly reactive substrate peptides for transglutaminases International conference

    Gordon Research Conference 2012 

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    Event date: 2012.7

    Language:English   Presentation type:Oral presentation (general)  

    Country:Japan  

  6. トランスグルタミナーゼが触媒するタンパク質のポリアミン修飾による生理機能の調節

    人見清隆

    日本農芸化学会大会シンポジウム 

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    Event date: 2012.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  7. タンパク質架橋化酵素トランスグルタミナーゼの高反応性基質配列の探索と多面的活用

    人見清隆

    酵素工学研究会第66回講演会 

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    Event date: 2011.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  8. Identification of preferred substrate sequecens for tranglutaminase and utility for detection of activity in an isozyme-specific manner

    Kiyotaka Hitomi

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    Event date: 2011.9

    Language:English   Presentation type:Oral presentation (general)  

    Country:Japan  

  9. Identification of highly reactive substrate peptides for transglutaminases from random peptide library and their applications International conference

    Gordon Research Conference: Trasnglutaminase in human diesease processes 

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    Event date: 2010.7

    Language:English   Presentation type:Oral presentation (invited, special)  

  10. Identification and Application of Highly Reactive Substrate Peptides for Transglutaminases

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    Event date: 2009.10

    Language:English   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  11. Characterization of the preferred substrate sequence for transglutaminase, an enzyme for protein cross-linking and polyamine-conjugation International conference

    Gordon Research Conference 2009 Polyamines 

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    Event date: 2009.6

    Language:English   Presentation type:Oral presentation (invited, special)  

  12. タンパク質架橋酵素・トランスグルタミナーゼの反応機構解析と応用

    日本農芸化学会2008年度大会シンポジウム 

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    Event date: 2008.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  13. Identification and characterization of the preferred substrate sequences for transglutaminases International conference

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    Event date: 2007.12

    Language:English   Presentation type:Oral presentation (general)  

    Country:Japan  

  14. Screening and identification of the preferred substrate sequences for transglutaminases using phage-displayed peptide library International conference

    The 9th Transglutaminase and cross-linking proteins International Conference 

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    Event date: 2007.9

    Language:English   Presentation type:Oral presentation (general)  

  15. Screening and Identification of the Preferred Substrate Sequences of Factor XIII and Transglutmainase 2 International conference

    The 51th annual meeting society of thrombosis and Haemostasis research/ Factor XIII symposium 

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    Event date: 2007.2

    Language:English   Presentation type:Oral presentation (invited, special)  

  16. トランスグルタミナーゼの最小基質配列の解明とその活用

    ポリアミンと核酸の共進化(第5回シンポジウム) 

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    Event date: 2006.11

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  17. Application of protein cross-linking enzyme, transglutamianse

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    Event date: 2005.11

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  18. トランスグルタミナーゼの傷害応答時の基質および基質認識機構の解析

    人見清隆

    第1回 ポリアミンとトランスグルタミナーゼ合同学術集会 

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    Event date: 2005.10

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  19. Analysis of transglutaminase substrates upon physical damage in slime mold International conference

    The 8th Transglutaminase and cross-linking proteins International Conference 

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    Event date: 2005.9

    Language:English   Presentation type:Oral presentation (general)  

  20. Immunological analysis of epidermal-type transglutaminase (TGase 3) in epithelium

    Japanese Animal and Cell Technology 

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    Event date: 2004.11

    Language:English   Presentation type:Poster presentation  

    Country:Japan  

  21. Characterization of Slime Mold Transglutaminase and Cross-linking in Wound Healing

    人見清隆

    第76回日本生化学会大会シンポジウム 

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    Event date: 2003.10

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

    "パシフィコ横浜, 横浜市"

  22. Molecular cloning and characterization of transglutaminase in slime mold-possible role for wound healing International conference

    the 2nd Japan-Hungary Joint conference on Transglutaminase in Heviz 

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    Event date: 2003.9

    Language:English   Presentation type:Oral presentation (invited, special)  

  23. 皮膚表皮のトランスグルタミナーゼの活性制御機構とその応用

    人見清隆

    第4回酵素応用シンポジウム 

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    Event date: 2003.6

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

    "名鉄ニューグランドホテル, 名古屋"

  24. Molecular cloning and characterization of transglutaminase in slime mold (Physarum polycephalum) International conference

    7th International Conference on Transglutaminase and protein cross-linking reactions 

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    Event date: 2002.9

    Language:English   Presentation type:Oral presentation (general)  

    Ferrara Italy

  25. 皮膚表皮に存在するカルシウム依存性蛋白質架橋化酵素の発現と活性調節機構に関する研究

    人見清隆

    日本農芸化学会関東・中部支部合同例会受賞講演 

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    Event date: 2002.6

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

    "松本文化会館, 松本"

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Works 3

  1. 分子生物学・免疫学キーワード辞典

    2003

  2. 第6回トランスグルタミナーゼとタンパク架橋に関する国際会議に参加して

    2001

  3. 学会見聞記:第10回日本動物細胞工学会国際会議

    1998

Research Project for Joint Research, Competitive Funding, etc. 11

  1. 皮膚の形成と疾患に関わるタンパク質接着反応の可視化システム

    2012.12 - 2013.11

    テルモ科学技術振興財団一般研究助成 

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    Grant type:Competitive

    皮膚形成では、表皮細胞の分化に伴ってケラチン等の様々なタンパク質が接着(架橋)して不溶化し、表皮細胞膜を裏打ちすることにより皮膚の硬度が保たれている。この接着反応を担うのが、タンパク質接着酵素(トランスグルタミナーゼ)で、遺伝子欠損も含め異常な活性レベルは多様な皮膚疾患をもたらす。またこの酵素反応は細胞外マトリクス(ECM)の強化による傷害修復にも寄与している。そのため各種皮膚疾患の再生回復には本酵素の活性上昇が必須である。
     これまで申請者は、接着酵素に対する「高反応性基質ペプチド」をペプチドライブラリから同定して多面的に活用し、蛍光標識した基質ペプチドを用いて、組織での接着反応を可視化するシステムを確立した。
     本研究ではその成果を基に、遺伝的要因による皮膚疾患や皮膚再生における接着反応(産物)の可視化システムを展開・活用し、病的皮膚形成への対処法と創薬シーズ開発のための情報基盤を提供する

  2. タンパク質接着酵素反応の可視化システムの多面的活用

    2012.11 - 2013.10

    研究成果最適展開支援プログラム(A-STEP) 探索タイプ 

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    Grant type:Competitive

    動物の生体内にはタンパク質を接着させる酵素反応が存在する。これらは血液凝固や皮膚形成、傷害修復など多彩な生命現象に関与し、その異常な活性レベルは疾患症状をもたらす。申請者は本酵素を対象に、天然に存在するタンパク質基質よりも遥かに反応性の高い「高反応性基質ペプチド」をペプチドライブラリから同定してきた。また、これらを蛍光標識したペプチドを用い、組織内の接着反応の可視化に成功している。この成果を基に、1)蛍光共鳴現象(FRET)による細胞内活性の可視化、2)疾患診断への広汎な活用を目指す。これらの高反応性基質ペプチドの可視化システムの活用は、疾患要因の特定や傷害修復における有効な因子探索に寄与する。

  3. コスメトロジー研究振興財団(皮膚表皮形成に関わるタンパク質架橋接着反応の分子基盤)

    2007.12

    コスメトロジー研究振興財団 

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    Grant type:Competitive

    コスメトロジーにおける生体作用、安全性に関する研究分野での研究助成

  4. Transglutaminase substrate preferred sequence and its application of protein immobilization

    2006.9 - 2007.2

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    Grant type:Competitive

  5. Characterization of higher reactivity sequence of transglutaminase for protein cross-linking

    2005.12 - 2007.9

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    Grant type:Competitive

  6. 皮膚表皮におけるタンパク質架橋化酵素の解析と応用

    2003.7 - 2005.6

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    応用酵素シンポジウム奨励賞の副賞として

  7. 武田科学振興財団(皮膚表皮の防御システム構築に関わるカルシウム依存性タンパク質架橋反応)

    2003.6

    武田科学振興財団一般研究助成 

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    Grant type:Competitive

    委任経理金

  8. 国際交流助成(蛋白質架橋化酵素の皮膚表皮分化に伴う活性化機構に関する研究)

    2001.6 - 2001.8

    長瀬科学財団 

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    Grant type:Competitive

    委任経理金

  9. 海外派遣助成(第6回トランスグルタミナーゼ国際会議参加補助)

    2000.9

    名古屋大学学術振興基金 

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    Grant type:Competitive

    委任経理金

  10. 国際会議参加補助金

    2000.9

    農芸化学研究奨励金 

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    Grant type:Competitive

    委任経理金

  11. カルパインサブユニットファミリーの機能解析

    1998.6 - 2002.1

    医薬資源研究奨励金 

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    Grant type:Competitive

    委任経理金

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KAKENHI (Grants-in-Aid for Scientific Research) 15

  1. 表皮培養と胎児皮膚形成の特殊性に着目した分化機構と羊水成分の解明

    Grant number:20K20568  2020.8

    科学研究費補助金  挑戦的研究(開拓)

    人見清隆

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    Authorship:Principal investigator  Grant type:Competitive

    Grant amount:\8710000 ( Direct Cost: \6700000 、 Indirect Cost:\2010000 )

  2. 上皮組織の硬度化を司るタンパク質架橋酵素反応の統合的分子基盤

    Grant number:18H02134  2018.4 - 2021.3

    科学研究費補助金  基盤研究(B)

    人見清隆

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    Authorship:Principal investigator  Grant type:Competitive

    Grant amount:\15020000 ( Direct Cost: \13400000 、 Indirect Cost:\1620000 )

  3. 生体機能を司る蛋白質架橋化とその制御破綻に関する分子基盤

    2014.4 - 2017.3

    科学研究費補助金  基盤研究(B)

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    Authorship:Principal investigator 

  4. 血栓形成と止血剤の研究を支援する血液凝固酵素の高反応性基質ペプチド

    2013.4 - 2015.3

    科学研究費補助金 

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    Authorship:Principal investigator 

  5. Molecular characterization of protein cross-linking reaction for cellular functions

    2011.7 - 2014.3

    Grant-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research(B)

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    Authorship:Principal investigator 

  6. Studies on utitlities of high reactivity substrate peptides for glue-peptide

    2011.7 - 2013.3

    Grant-in-Aid for Scientific Research 

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    Authorship:Principal investigator 

  7. Multiple application of cross-linking enzyme activity in life science

    2008.11 - 2011.3

    Grant-in-Aid for Scientific Research 

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    Authorship:Principal investigator 

  8. タンパク質架橋反応を担う酵素群の最小基質配列の解明と活用

    2007.7 - 2009.3

    科学研究費補助金  基盤研究(C)(一般),課題番号:19580103

    人見 清隆

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    Authorship:Principal investigator 

  9. Molecular basis of formation of corneocyte in skin epidermis

    2005.7 - 2007.2

    Grant-in-Aid for Scientific Research 

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    Authorship:Principal investigator 

  10. 高効率物質生産システムとしての浮遊-付着可変型動物細胞培養系の開発

    1997.7 - 2000.2

    科学研究費補助金  萌芽研究・09876023

    人見清隆

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    Authorship:Principal investigator 

  11. 皮膚表皮細胞内の架橋化反応を制御するカルシウム依存性機能蛋白質に関する研究

    2002.7 - 2004.1

    科学研究費補助金  基盤研究(C)(2)(一般)

    人見

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    Authorship:Principal investigator 

  12. Analysis of activation mechanism of transglutaminase in skin epidermis

    2000.7 - 2002.1

    Grant-in-Aid for Scientific Research 

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    Authorship:Principal investigator 

  13. 食品由来の骨形成調節因子の単離とその構造解析

    1996.7 - 1997.2

    科学研究費補助金  奨励研究(A)・08760131

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    Authorship:Principal investigator 

  14. アスコルビン酸-輸送担体に関する蛋白化学的研究

    1993.7 - 1994.2

    科学研究費補助金  奨励研究A05760107・

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    Authorship:Principal investigator 

  15. アスコルビン酸による骨形成効果の培養細胞系での解

    1992.7 - 1993.2

    科学研究費補助金  奨励研究A・04760061

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    Authorship:Principal investigator 

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Industrial property rights 2

  1. トランスグルタミナーゼ基質反応性を有するペプチド及びその利用(2005-380040に基づく優先権主張に伴う特許出願)

    人見清隆、杉村禎昭、牧正敏

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    Applicant:名古屋大学

    Application no:2006-352448  Date applied:2006.12

    Announcement no:2007-197434 

    Country of applicant:Domestic  

    2005年に出願した特許内容のさらなる追加内容として、固定化酵素への応用可能であることを示すデータを追加した。

  2. トランスグルタミナーゼ基質反応性を有するペプチド及びその利用

    人見清隆、杉村禎昭、牧正敏

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    Applicant:名古屋大学

    Application no:2005-380040  Date applied:2005.12

    Country of applicant:Domestic  

    ペプチドライブラリーを用いた、トランスグルタミナーゼの高反応性配列を検索する実験系を確立し、基質となりやすい様々なペプチド配列を見出した

 

Teaching Experience (On-campus) 8

  1. 先端生物科学

    2012

  2. 細胞生物学3

    2012

  3. 創薬探索科学

    2012

  4. 基盤創薬学概論

    2012

  5. 基礎セミナー

    2012

  6. Biotechnology

    2011

  7. 細胞生物学3

    2009

     詳細を見る

    免疫化学・細胞の分化増殖・細胞外マトリクス

  8. 細胞生物学2

    2009

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Teaching Experience (Off-campus) 3

  1. 食品生物科学特論Ⅱ

    2016.4 - 2017.3 Kyoto University)

  2. 統合生命科学特別講義III

    2012.4 - 2013.3 Kyushu University)

  3. 農学特論

    2011.4 - 2012.3 Ibaraki University)