ワシントン大学歯学部客員研究員（文部科学省在外研究員）

2002.7 - 2002.8

ワシントン大学歯学部客員研究員

2001.6 - 2001.7

名古屋大学大学院生命農学研究科　助教授

1999.4

名古屋大学農学部応用生物科学科　助教授

1995.11 - 1999.3

名古屋大学農学部食品工業化学科　助手

1989.10 - 1995.11

京都大学教養部非常勤講師

1989.4 - 1989.9

日本学術振興会特別研究員

1988.4 - 1989.9

Japan Society for Cell Biology

日本分子生物学会

Biochemical characterization of medaka (Oryzias latipes) fibrinogen gamma and its gene disruption resulting in anemia as a model fish Reviewed

Meng Qi, Yuko Watanabe, Hideki Tatsukawa, Hisashi Hashimoto, Kiyotaka Hitomi

Journal of Biochemistry   Vol. 172 ( 5 ) page： 293 - 302   2022.10

Investigation of mouse amniotic fluid for stimulating ability of keratinocyte differentiation depending on the fetal stage. Invited Reviewed

Archives Biochemistry Biophysics   Vol. 711   page： 1   2021.10

Spatially Resolved Identification of Transglutaminase Substrates by Proteomics in Pulmonary Fibrosis Invited Reviewed

American Journal of Respiratory Cell Molecular Biology   Vol. 65   page： 319 - 330   2021.9

Role of Transglutaminase 2 in Cell Death, Survival, and Fibrosis Invited Reviewed

Cells   Vol. 10 ( 7 ) page： 1842   2021.7

Thrombin-deficient mutant of medaka, a model fish, displays serious retardation in blood coagulation Reviewed

Watanabe Y., Oguri R., Suzuki R., Ishikawa Y., Tatsukawa H., Hashimoto H., Hitomi K.

Bioscience Biotechnology Biochemistry   Vol. 85 ( 4 ) page： 824 - 833   2021.4

Identification and characterization of substrates crosslinked by transglutaminases in liver and kidney fibrosis Reviewed

Tatsukawa Hideki, Takeuchi Taishu, Shinoda Yoshiki, Hitomi Kiyotaka

ANALYTICAL BIOCHEMISTRY   Vol. 604   page： 113629   2020.9

Transglutaminase orthologues in medaka fish - biochemical characterization and establishment of gene-deficient mutants Reviewed

Meng Qi, Watanabe Yuko, Suzuki Risa, Oguri Rina, Tatsukawa Hideki, Hitomi Kiyotaka

ANALYTICAL BIOCHEMISTRY   Vol. 604   page： 113610   2020.9

Analysis of the expression of transglutaminases in the reconstructed human epidermis using a three-dimensional cell culture Reviewed

Teshima Hirofumi, Kato Manami, Tatsukawa Hideki, Hitomi Kiyotaka

ANALYTICAL BIOCHEMISTRY   Vol. 603   page： 113606   2020.8

Gene disruption of medaka (Oryzias latipes) orthologue for mammalian tissue-type transglutaminase (TG2) causes movement retardation. Reviewed

Watanabe Y., Okuya K., Takada Y., Kinoshita M., Yokoi S., Chisada S, Kamei Y., Tatsukawa H., Yamamoto N., Abe H., Hashimoto H., Hitomi K.

Journal of biochemistry   Vol. 168 ( 3 ) page： 213 - 222   2020.4

Studies on differentiation-dependent expression and activity of distinct transglutaminases by specific substrate peptides using three-dimensional reconstituted epidermis.

Tanabe Y, Yamane M, Kato M, Teshima H, Kuribayashi M, Tatsukawa H, Takama H, Akiyama M, Hitomi K

The FEBS journal   Vol. 286 ( 13 ) page： 2536-2548   2019.7

Detection and identification of potential transglutaminase 2 substrates in the mouse renal glomeruli

Ito Yoshimasa, Tatsukawa Hideki, Yamaguchi Hisateru, Takahashi Kazuo, Hitomi Kiyotaka, Yuzawa Yukio

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS   Vol. 660   page： 11-19   2018.12

ISOZYME-SPECIFIC IDENTIFICATION AND CHARACTERIZATION OF SUBSTRATES CROSSLINKED BY TRANSGLUTAMINASES IN LIVER FIBROSIS

Tatsukawa H., Nakagawa H., Hitomi K.

ALCOHOLISM-CLINICAL AND EXPERIMENTAL RESEARCH   Vol. 42   page： 33A-33A   2018.8

Higher susceptibility to osmolality of the medaka (Oryzias latipes) mutants in orthologue genes of mammalian skin transglutaminases. Reviewed

Watanabe Y., Furukawa E. Tatsukawa H., Hashimoto H., Kamei Y., Taniguchi Y., Hitomi K.,

Biosci. Biotechnol. Biochem   Vol. 82 ( 7 ) page： 1165-1168   2018.7

A granular parakeratosis animal model created by topical aluminum showed characteristic keratinocyte differentiation abnormalities including abnormal profilaggrin processing and cornified cell envelop formation

Fujii M., Kishibe M., Ueda J., Hitomi K., Ishida-Yamamoto A.

JOURNAL OF INVESTIGATIVE DERMATOLOGY   Vol. 138 ( 5 ) page： S68-S68   2018.5

Isozyme-specific comprehensive characterization of transglutaminase-crosslinked substrates in kidney fibrosis. Reviewed

Tatsukawa H, Otsu R, Tani Y, Wakita R, Hitomi K.

Sci Rep   Vol. 9 ( 8 ) page： 7306   2018.5

Biochemical characterization of medaka (Oryzias latipes) orthologue for mammalian Factor XIII and establishment of gene-edited mutant. Reviewed

Horimizu R, Ogawa R, Y Watanabe, Tatsukawa H, Kinoshita M, Hashimoto H, and Hitomi K.

  Vol. 284   page： 2843-2855   2017.9

Global identification and analysis of isozyme-specific possible substrates crosslinked by transglutaminases using their substrate peptides in mouse liver fibrosis. Reviewed

Tatsukawa H., Tani Y., Otsu R., Nakagawa H., Hitomi K.

  Vol. 7   page： 45049   2017.3

Characterization of medaka orthologue of human transglutaminase 2 (OlTGT). Reviewed

Takada Y., Watanabe Y., Okuya K., Tatsukawa H., Hashimoto H, Hitomi K

Biosci. Biotechnol. Biochem.   Vol. 81 ( 3 ) page： 469-474   2017.3

FRET-based detection of isozyme-specific activities of transglutaminases

Tatsukawa H. Liu HH., Ooba S., Kamiya N., Nakanishi Y., Hitomi K.

Amino Acids   Vol. 49   page： 615-623   2017.5

Analysis on transglutaminase 1 and its substrates using specific substrate peptide in cultured keratinocytes. Reviewed

Yamane M., Sugimura K., Kawasaki H., Tatsukawa H., Hitomi K.

Biochemical Biophysical Research Comunications   Vol. 478   page： 343-348   2016.9

Biochemical characterization of medaka (Oryzias latipes) transglutaminases, OlTGK1 and OlTGK2, as orthologues of human keratinocyte-type transglutaminase.

A Kikuta, E Furukawa, R Ogawa, N Suganuma, M Saitoh,T Nishimaki, T Katsumura, Hi Oota, T Kawamoto, H Tatsukawa H Hashimoto and K Hitomi

PLOS One     2015.12

Distribution of transglutaminase family members in mouse tissues and whole body sections.

Tatsukawa H., Abe N., Ohashi S., and Hitomi K.

Biochem Biphys Res Commun.   Vol. 467   page： 10146-1051   2015.11

Transglutaminase activity is decreased in large arteries from hypertensive rats, compared to normotensive controls. Reviewed

Petersen-Jones HG, Johnson KB, Hitomi K, Tykocki NR, Thompson JM, Watts SW.

Am J Physiol Heart Circ Physiol.   Vol. 308   page： 592-602   2015.3

Early response as shown by enhancement of transglutaminase 1 expression after cisplatin-induced acute kidney injury. Reviewed

Furukawa K., Yamane M., Tatsukawa H., Hitomi K.

Arch Biochem Biophys.   Vol. 586   page： 27-32   2015.9

Transglutaminase activity is decreased in large arteries from hypertensive rats compared with normotensive controls. Reviewed

Petersen-Jones HG, Johnson KB, Hitomi K, Tykocki NR, Thompson JM, Watts SW.

Am J Physiol Heart Circ Physiol.   Vol. 308   page： 592-602   2015.3

Tissue Transglutaminase Mediated Tumor-Stroma Interaction Promotes Pancreatic Cancer Progression. Reviewed

Lee J, Condello S., Yakubov B., Emerson R., Caperell-Grant A., Hitomi K., Xie J., Matei D.

Clin Cancer Res.   Vol. 21   page： 4482-4493   2015.8

Lamellar ichthyosis with pseudoexon activation in the transglutaminase 1 gene Reviewed

Suga Y., Tsuda T., Nagai M., Sakaguchi Y., Jitsukawa O., Yamamoto M., Hitomi K., Yamanishi K.

J. Dermatology   Vol. 42   page： 642-645   2015

Hypohidrosis plays a crucial role in the vicious circle of bathing suit ichthyosis: a case with summer exacerbation Reviewed

Washio K, Fukunaga A, Terai M, Hitomi K, Yamanishi K, Nishigori C

Acta Dermato-Venerelogica   Vol. 94   page： 349-350   2014.5

Transglutaminase activity arising from Factor XIIIA is required for stabilization and conversion of plasma fibronectin into matrix in osteoblast cultures. Reviewed

C. Cui, Wang S., Myneni VD, Hitomi K., Kaartinen M.

Bone   Vol. 59   page： 127-138   2014.2

Identification of a highly reactive substrate peptudei for transglutaminase 6 and its use in detecting TGase activity in the skin epidermis Reviewed

Fukui M., Kuramoto K., Yamasaki R., Shimizu Y., Itoh M., Kawamoto T., Hitomi K.

  Vol. 280   page： 1420-1429   2013.1

Phage-displayed peptide liberary screening for preferred human substrate peptide sequences for transglutaminase 7 Reviewed

Kuramoto K., Yamasaki R., Shimizu Y., Tatsukawa H., Hitomi K

Archives Biochemistry and Biochemistry   Vol. 537 ( 1 ) page： 138-143   2013

Variations in both TG1 and TG2 isozyme-specific in situ activities and protein expressions during mouse embryonic development. Reviewed

Itoh M., Tatsukawa H., Lee E-S, Yamanishi K., Kojima S., and Hitomi K.

J. Histochem. Cytochem.   Vol. 61   page： 793-801   2013.11

Spatial arrangement of proteins using scCro-tag: application for an in situ enzymatic microbead assay

Kojima Takaaki, Hata Jumpei, Oka Hiroya, Hayashi Kenta, Hitomi Kiyotaka, Nakano Hideo

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY   Vol. 82 ( 11 ) page： 1911-1921   2018.11

Targeting transglutaminase 2 partially restores extracellular matrix structure but not alveolar architecture in experimental bronchopulmonary dysplasia

Mizikova Ivana, Pfeffer Tilman, Nardiello Claudio, Solaligue David E. Surate, Steenbock Heiko, Tatsukawa Hideki, Silva Diogo M., Vadasz Istvan, Herold Susanne, Pease Richard J., Iismaa Siiri E., Hitomi Kiyotaka, Seeger Werner, Brinckmann Juergen, Morty Rory E.

FEBS JOURNAL   Vol. 285 ( 16 ) page： 3056-3076   2018.8

Factor XIII-A transglutaminase acts as a switch between preadipocyte proliferation and differentiation. Reviewed

Myneni VD, Hitomi K., Kaartinen M.

Blood   Vol. 124   page： 1344-1353   2014

Distinct protein expression and activity of transglutaminases found in different epidermal tumors. Reviewed

Karashima T., Furumura M., Ishii N., Ohyama B., Saruta H., Natsuaki Y., Ohata C., Tsuruta D., Hitomi K., and Hashimoto T.

Exp. Dermatol.   Vol. 23   page： 433-435   2014

Detyrosinated Glu-tubulin is a substrate for cellular Factor XIIIA transglutaminase in differentiating osteoblast. Reviewed

Wang, S., Cui C., Hitomi K., Kaartinen M.

Amino Acids   Vol. 46   page： 1513-1526   2014

タンパク質架橋化酵素の高反応性基質の探索と活用 Invited

人見清隆

バイオサイエンスとバイオインダストリー   Vol. 70 ( 6 ) page： 442-447   2012.11

A distinct effect of transient and sustained upregulation of cellular factor XIII in the goldfish and optic nerve on optic nerve regeneration Reviewed

Sugitani, K., Ogai K., Hitomi, K., Nakamura-Yonehara K., Shintani T., Noda M., Kato S.

Neurochem. Int.   Vol. 61   page： 423   2012.8

Vena cava and aortic smooth muscle cells express transglutaminase 1 and 4 in addition to tranglutaminase 2 Reviewed

Johnson KB, Hitomi K, Itoh M, Bakker EN, , Watts SW et al.

American Journal of Physiology (circulation physiology)   Vol. 302 ( 7 ) page： H1355-1366   2012.4

Knocking-in the R142C mutation in transglutaminase 1 disrupts the stratum corneum barrier and postnatal survival of mice Reviewed

Nakagawa N., Yamamoto M., Imai Y., Sakaguchi Y., Takizawa T., Ohta N., Yagi N., Hatta I., Hitomi K., Takizawa T., Takeda J., Tsuda T., Matsuki M. Yamanishi K.

Journal of Dermatological Sciences   Vol. 65 ( 3 ) page： 196-206   2012.3

Bathing suit ichthyosis with summer exacerbation: a temperature-sensitive case Reviewed

Yamamoto M., Sakaguchi Y., Itoh M., Nakagawa N., Fukunaga A., Hitomi K., Yamanishi K.

Biritish Journal of Dermatology   Vol. 166 ( 3 ) page： 671-693   2012.3

Hornerin is a component of the epidermal cornified cell envelopes Reviewed

Henry J, Hsu C-Y, Haftek, M., Nachat R.,de Koning HD., Gardina-Galera I, Hitomi K. Balica, S., Jean-Decoster, C., Schmitt, A-M., Serre, G., SimonM

FASEB Journal   ( 25 ) page： 1567-1576   2011.5

Screening of substrate peptide sequences for tissue-type transglutaminase (TGase 2) Using T7 Phage cDNA Library. Reviewed

Sugimura Y., Yamashita H, Hitomi K.

Cytotechnology   ( 63 ) page： 111-118   2011.3

In situ detection of active transglutaminases for keratinocyte-type (TGase 1) and tissue-type (TGase 2) using fluorescence-labeled highly reactive substrate peptides. Reviewed

Itoh M., Kawamoto T., Tatsukawa H., Kojima S., Yamanishi, K., Hitomi, K.

Journal of Histochemistry and Cytochemistry   ( 59 ) page： 180-187   2011.2

Identification of a preferred substrate peptide for transglutaminase 3 and detection of in situ activity in skin and hair follicles Reviewed

Yamane, A., Fukui, M., Sugimura Y., Itoh, M., Prez Alea P., Thomas V., El Alaoui S., Akiyama M., Hitomi K.

FEBS Journal   Vol. 277   page： 3564–3574   2010.9

The cystatin M/E-cathepsin L balance is essential for tissue homeostasis in epidermis, hair follicles, and cornea. Reviewed

Zeeuwen PM., van Vlijmen-Willems I., Cheng, T., Rodijk-Olthuis1, D., Hitomi K., Hara-Nishimura I., John S. Smyth, N., Reinheckel T., Hendriks WJ., and Schalkwijk J.

FASEB J.   Vol. 24   page： 3744-3755   2010.5

Transglutaminase1 Preferred Substrate Peptide K5 Is an Efficient Tool in Diagnosis of Lamellar Ichthyosis. Reviewed

Masashi Akiyama, Kaori Sakai, K., Teruki Yanagi, T., Fukushima, S., Ihn H., Hitomi K., and Hiroshi Shimizu, H.

Am. J. Pathol.   Vol. 129   page： 2306-2309   2010.4

*A specific colorimetric assay for measuring teranglutamianse 1 and factor XIII activities. Reviewed

Analytical Biochemistry   Vol. 394   page： 281-283   2009.11

A biochemical pathway that controls skin barrier formation: expression of its key components in inflammatory skin diseases and in reconstructed skin. Reviewed

T. Cheng, G.S. Tjabringa, I.M.J.J. van Vlijmen-Willems, K. Hitomi*, P.E.J. van Erp, J.Schalkwijk, and P.L.J.M. Zeeuwen

Br. J. Dermatol.   Vol. 161   page： 253-264   2009.8

Development of an isoenzyme-specific colorimetric assay for tissue transglutaminase 2 cross-linking activity Reviewed

Perez Alea M., Kitamura, M., Martin, G., Thomas, V., Hitomi, K., El Alaoui,S.

Anal. Biochem.   Vol. 389   page： 150-156   2009.6

Colocalization of cystatin M/E and its target proteases suggests a role in terminal differentiation of human hair follicle and nail Reviewed

Cheng, T., van Vlijmen-Willems, IM, Hitomi, K., Schlkwijk, J. Zeeuwen, P.

J. Invest. Dermatol.   Vol. 129 ( 5 ) page： 12132-1242   2009.5

*Identification of preferred substrate sequences for transglutaminase 1: development of a novel peptide that can efficiently detect cross-linking enzyme activity in the skin. Reviewed

Sugimura Y, Hosono M, Kitamura M, Tsuda T, Yamanishi K, Maki M and Hitomi K.

FEBS Journal   Vol. 275   page： 5667-5677   2008.11

Identification of the preferred substrate sequences of microbial transglutaminase from Streptomyces mobaraensis using a phage-displayed peptide library. Reviewed

Sugimura Y., Yokoyama K., Nio N., Maki M., and Hitomi K.

Arch. Biophys. Biochem.   Vol. 477   page： 379-383   2008.6

Transglutaminase 2 activity promotes membrane resealing after mechanical damage in the lung cancer cell line A549 Reviewed

Kawai Y. Wada F., Sugimura Y., Maki M., and Hitomi K

Cell Biol. Int.   Vol. 32   page： 928-934   2008.4

Gene expression signatures that classify the mode of invasion of primary oral squamous cell carcinomas. Reviewed

Kondoh N, Ishikawa T, Ohkura S, Arai M, Hada A, Yamazaki Y, Kitagawa Y, Shindoh M, Takahashi M, Ando T, Sato Y, Izumo T, Hitomi K, Yamamoto M.

Mol. Carcinogen.   Vol. 47   page： 744-756   2008.4

Human calpain 7/PalBH associates with a subset of ESCRT-III-related proteins in its N-terminal region and partly localizes to endocytic membrane compartments Reviewed

Yorikawa C., Takaya E., Ohsako Y., Terasawa, Y., Hamakubo T., Mochizuki Y., Iwanari H., Kodama T., Maeda T., Hitomi K., Shibata H., Maki M.

J. Biochem.   Vol. 143   page： 731-735   2008.3

Novel site-specific immobilization of a functional protein using a preferred substrate sequence for transglutaminase 2 Reviewed

Y. Sugimura, H. Ueda, M. Maki, and K. Hitomi

Journal of Biotechnology   Vol. 131   page： 121-127   2007.8

*Identification of substrates for mammalian-type transglutaminase in Physarum polycephalum, an acellular slime mold, upon cellular mechanical damage Reviewed

Wada F., Hasegawa H., Hanai Y., Nakamura A., Sugimura Y. , Kawai Y., Sasaki N., Shibata H., Maki M. and Hitomi K

FEBS Journal   Vol. 274 ( 11 ) page： 2766-2744   2007.5

A novel strategy for generation of monoclonal antibodies from single B cells using rt-PCR technique and in vitro expression Reviewed

Jiang, X., Suzuki, H., Hanai, Y., Wada F., Hitomi, K., Yamane, T., Nakano, H

Biotechnol. Prog.   Vol. 22   page： 979-988   2006.7

Cystatin M/E is a high affinity inhibitor of cathepsin V and cathepsin L by a reactive site that is distinct from the legumain-binding site Reviewed

Cheng, T., Hitomi, K., Vlijmen-Willems, J. J., Jongh, G., J., Yamamoto K., Nihsi, K., Watts, C., Reinhecke, T., Schalkwijk, J. and P. Zeeuwen

The Journal of Biological Chemistry   Vol. 281 ( 23 ) page： 15893-15899   2006.6

*Screening for the preferred substrate sequence of transglutaminase using a phage-displayed peptide library Reviewed

Sugimura, Y., Hosono, M., Yoshimura, T., Maki, M., and Hitomi, K.

The Journal of Biological Chemistry   Vol. 281 ( 26 ) page： 17699-17706   2006.6

Generation of monoclonal antibodies using simplified single-cell reverse transcription-polymerase chain reaction and cell-free protein synthesis. Reviewed

Ali, M., Hitomi, K., and Nakano, H.

J. Biosci. Bioeng.   Vol. 101   page： 284-286   2006.3

Reevaluation of the predicted gene structure of dictyostelium cystatin A3 (cpiC) by nucleotide sequence determination of its cDNA and its phylogenetic position in the systatin superfamily Reviewed

El-halawany MS, Shibata, H., Hitomi, K., and Maki, M.

Molecular Biology Reports   Vol. 32 ( 4 ) page： 257-264   2005.12

Transglutaminase in the skin epidermis Invited Reviewed

  Vol. 15   page： 313-319   2005.9

"Analyses of expression and localization of two mammalian-type transglutaminases in Physarum Polycephalum, an acellular slime mold." Reviewed

"Wada, F., Ogawa, A., Hanai, Y., Nakamura, A. Maki, M., and Hitomi, K."

J. Biochem.   Vol. 136   page： 665-672   2004.11

"DdAlix, an Alix/AIP1 homolog in Dictyostelium discoideum, is required for multicellular development under low Ca2+ conditions." Reviewed

"Ohkouchi, S., Medhat, El-H., Maeda, M., Shibata, H., Hitomi, K., and Maki, M."

Gene   Vol. 337   page： 131-139   2004.10

Evidence that unrestricted legumain activity is involved in disturbed epidermal cornifcation in cystatin M/E deficient mice. Reviewed

"Zeeuwen, P.M., van Vlijmen-Willems, I., Olthuis1, D., Johansen, H.T., Hitomi, K., Hara-Nishimura, I., Powers, J. C., James, K. E., Camp, H. J., Lemmens1, R., and Schalkwijk1, J."

Hum. Mol. Genet.   Vol. 13   page： 1069-1079   2004.10

Identification of cysteine protease inhibitors that belong to cystatin family 1 in the cellular slime mold Dictyostelium discoideum. Reviewed

"Medhat, El-H., Ohkouti, S., Shibata, H., Hitomi, K., and Maki, M."

Biol. Chem.   Vol. 385   page： 547-550   2004.6

Immunological detection of proteolytically activated epidermal-type transglutaminase (TGase 3) using cleavage site specific antibody Reviewed

"Hitomi, K., Ikeda, N., and Maki, M."

Biosci. Biotechnol. Biochem.   Vol. 67   page： 2492-2494   2003.11

Analysis of epidermal-type transglutaminase in human stratified epithelia and cultured keratinocytes using monospecific antibodies Reviewed

"Hitomi, K., Nakayama, T., Presland, R.B., Fleckman, P., Dale, B. A., and Maki , M."

J. Dermatol. Sci.   Vol. 32   page： 95-103   2003.7

Molecular Evolution of Transglutaminase: from bacteria to animal Invited

"Hitomi, K."

Recent Research Developmemts in Biophysics and Biochemistry   Vol. 3   page： 223-234   2003.1

Identification of Mammalian-type Transglutaminase in Physarum polycephalum: Evidences from cDNA sequence and involvement of GTP in the regulation of transamidating activity Reviewed

"Wada, F., Nakamura, Masutani, T., A., Ikura, K., Maki, M., and Hitomi K."

Eur. J. Biochem.   Vol. 269   page： 3451-3460   2002.9

Identification and Characterization of Two Penta-EF-Hand Ca(2+)-Binding Proteins in Dictyostelium discoideum Reviewed

"Ohkouchi S., Nishio K., Maeda, M., Hitomi K., Adachi H., and Maki M."

J. Biochem (Tokyo).   Vol. 130   page： 207-215   2001.8

Analysis of Epidermal Transglutaminase (TGase3) Expression in Mouse Tissues and Cell Lines Reviewed

"Hitomi, K., Horio, Y., Ikura, K., Yamanishi, K., and Maki, M."

Int. J. Biochem. & Cell Biol   Vol. 33   page： 491-498   2001.6

"Peflin and ALG-2, Members of the Penta-EF-Hand Protein Family, Form a Heterodimer that Dissociates in a Ca2+-Dependent Manner"

"Kitaura, Y., Matumoto S., Satoh,H., Hitomi, K., and Maki, M."

J. Biol. Chem   Vol. 276   page： 14503-14508   2001.4

Characterization of human recombinant transglutaminase 1 purified from baculovirus-infected insect cells Reviewed

"Hitomi K., Ymagiwa, Y., Ikura, K., Yamanishi, K., and Maki, M."

Biosci. Biochem. Biochem.   Vol. 64   page： 2128-2137   2000.10

Four types of calpastatin isoforms with distinct amino-terminal sequences are specified by alternative first exons and differentially expressed in mouse tissues

"Takano, J., Watanabe, M., Hitomi, K., and Maki, M."

Journal of Biochemistry   Vol. 128   page： 83-92   2000.6

"GTP, an Inhibitor of Transglutaminases, is Hydrolyzed by Tissue-type Transglutaminase(TGase 2)"

"Hitomi, K., Ikura, K., and Maki, M."

Biochem. Biosci. Biotechnol.   Vol. 64   page： 657-659   2000.4

"Peflin, a novel member of the five-EF-hand-protein family, is similar to the Apoptosis-linked-gene 2 (ALG-2) protein but possesses nonapeptide repeats in the N-terminal hydrophobic region."

"Kitaura, Y., Sato, H., Watanabe, M., Kawai, T., Hitomi, K., and Maki, M."

Biochem. Biophys. Res. Commun   Vol. 263   page： 68-75   1999.10

Purification of recombinant calpastatin in Escherichia coli

"Maki, M. and Hitomi, K"

Methods in Molecular Biology   Vol. 144   page： 85-94   1999.1

Structure of mouse calpastatin isoforms:Implication of species-common and species-specific alternative splicing Reviewed

"Takano J., Kawamura T., Murase, M., Hitomi, K., and Maki, M."

Biochemcal and Biophysical Research Communication   Vol. 260   page： 339-345   1999.1

Characterization of recombinant mouse epidermal-type transglutaminase (TGase 3):Regulation of its activity by proteolysis and guanine nucleotides. Reviewed

"Hitomi, K., Kanehiro, S., Ikura, K., and Maki, M."

Journal of Biochemistry   Vol. 125   page： 1048-1054   1999.1

Preparation of calpastatin Samples for Western blotting

"Maki, M. and Hitomi, K."

Methods in Molecular Biology   Vol. 144   page： 95-98   1999.1

Purification of recombinant calpastatin in Escherichia coli Invited

Methods in Molecular Biology   Vol. 144   page： 85   1999

Preparation of calpastatin samples for Western blotting Invited

Methods in Molecular Biology   Vol. 144   page： 95   1999

Regulation of matrix metalloproteinase-9 a inhibition of tumor invasion by the membrane-anchored glycoprotein RECK. Reviewed

"Takahashi C., Sheng, Z., Horan, T. P., Kitayama, H., Maki, M., Hitomi, K., Kitaura, Y., Takai, S., Sasahara, R.M., Horimoto, R., Ikawa, Y., Ratzkin, B., Arakawa, T., and Noda, M.,"

Proceedings of the National Academy of Sciences of the U.S.A.   Vol. 95   page： 13221-13226   1998.10

Expression of biologically active human calpastatin in baculovirus-infected insect cells a in Ecsherichia coli. Reviewed

"Hitomi, K. , Yokoyama, A., and Maki, M.,"

"Bioscience, Biotechnology, a Biochemistry"   Vol. 62   page： 136-141   1998.1

Purification a characterization of the active-site-mutated recombinant human μ-calpain in baculovirus-infected Insect cells.

"Hitomi, K., Uchiyama, Y., Ohkubo, I., Kunimatsu, M., Sasaki, M., and Maki, M.,"

Biochemcal a Biophysical Research Communication   Vol. 246   page： 681-685   1998.1

Ascorbate-depeent enhancement of nitric oxide formation in activated macrophages

"Mizutani, A., Maki, H., Torii, Y., Hitomi, K., and Tsukagoshi, N.,"

Nitric Oxide   Vol. 2   page： 235-241   1998.1

Calcium-iuced exposure of hydrophobic surface on mouse ALG-2 which is a member of the penta-EF-ha protein family.

"Maki, M., Yamaguchi, K., Kitaura, Y., Sato, H., and Hitomi, K.,"

Journal of Biochemistry (Tokyo)   Vol. 124   page： 1170-1177   1998.1

A growing family of the calcium-binding proteins with five EF-hand motifs

"Maki, M., Narayam, A.V.L., and Hitomi, K"

Biochem. J   Vol. 328   page： 718-720   1997.1

Functional properties of recombinant calpain I a of mutants lacking domains III a IV of the catalytic subunit.

"Vilei, E.M. , Calderara, S., Anagli, J., Berardi, S., Hitomi, K., Maki, M., and Carafoli, E.,"

Journal of Biological Chemistry   Vol. 272   page： 25802-25808   1997.1

Ascorbate-dependent expression of ubiquitin genes in guinea pigs. Reviewed

"Mizutani, A., Nagkagawa, N., Hitomi, K., and Tsukagoshi, N.,"

International Journal of Biochemistry a Cell Biology   Vol. 29   page： 575-582   1997.1

Functional properties of reconbinant calpain I and of mutants lacking domains III and IV of the catalyfic subunit(共著) Reviewed

J. Biol. Chem.   Vol. 272   page： 25802   1997

Ascorbic acid:Role of Ascorbic Acid on Modulation of Gene Expression

"Hitomi, K. and Tsukagoshi, N."

Subcellular Biochemistry (Plenum Publishing Co.)   Vol. 25   page： 41-56   1996.1

Demonstration of alkaline phosphatase participation in the mineralization of osteoblasts by antisense RNA approach.

"Torii Y., Hitomi K., Yamagishi Y., and Tsukagoshi N.,"

Cell Biology International   Vol. 20   page： 459-464   1996.1

4-hydroxy-2-nonenal cytotoxitciy in renal proximal tubular cells: Protein modification a redox alteration.

"Fukuda, A., Osawa, T., Hitomi, K., and Uchida, K.,"

Archives of Biochemistry a Biophysics   Vol. 333   page： 419-426   1996.1

Synergistic effect of BMP-2 a ascorbate on the phenotypic expression of osteoblastic MC3T3- E1. Reviewed

"Torii Y., Hitomi K., and Tsukagoshi N.,"

Molecular and Cellular Biochemistry   Vol. 165   page： 25-29   1996.1

cDNA sequence for rKST-1, a novel member of the sodium ior dependent glucose cotransporfer family Reviewed

Biochimica et Biophysica Acta   Vol. 1190 ( 2 ) page： 469   1994

L-Ascorbic Acid 2-Phosphate promotes osteoblastic differentiation of MC3T3-E1 Mediated by Accumulation of Type I Collagen (共著)

Journal of Nutritional Science and Vitaminology   Vol. 40   page： 229-238   1994

Ascorbate-dependent elevation of mRNA levels for cytochrome P450s induced by polychlorinated biphenyls

Biochemical Pharmacology   Vol. 46 ( 1 ) page： 186   1993

Increase in the activity of Alkaline Phosphatase by L-Ascorbic Acid 2-phosphate in a Human Oskoblast Cell Line, HuO-3N1

Journal of Nutritional Science and Uitaminology   Vol. 38   page： 535   1992

Recombinant Human Erythropoietir and Its Receptor

Trends in Animal Cell Culture Technology     1990

Establishment of an erythroid cell line (JK-1) that spontaneously differentiation to red cells(共著)

Cancer   Vol. 66   page： 1544-1541   1990

Expression of the Erythropoietin Receptor on a Human Myeloma Cell Line

Biochemical Biophysical Research Communication     page： 170   1990

Solubilization and Characterization of Erythropoietin Receptor from Transplantable Mouse Erythroblastic Leukemic cell Line.

Biochemical Biophysical Research Communication     page： 160   1989

Continuous Production of erythropoietin with Immobilized Animal Cells(共著)

Applied Microbiology Biotechnology   Vol. 29   page： 544   1988

Stromal Cell-dependent Growth of Leukemic Cells from Murine Erythroblastic Leukemia(共著)

Japanese Journal of Cancer Research   Vol. 79   page： 931   1988

Production of Erythropoietin by BHK Cells Growing on the Microcarriers Trapped in Alginate Cells(共著)

Cytotechnology   Vol. 2   page： 141-145   1988

Erythropoietin Receptor of a Human leukemic Cell Line with Erythroid Characteristics

Biochemical Biophysical Research Communication     page： 79   1988

食品・医薬品分野における蛋白質テーラリング(共訳)

学会出版センター     1988

Characterization of Erythropoietin Receptor of Murine Erythroid Cells.

European Journal of Biochemistry     page： 168   1987

Isolation and Characterization of cDNA Encoding Rabbit Reticulocyte 2,3-Bisphospho-glycerate synthase

Gene     page： 44   1986

Identification and application of the preferred substrate peptides for transglutaminases

（ Role： Sole author）

2009.8 

*Preferred substrate sequences for transglutaminase 2: screening using a phage-displayed peptide library

Hitomi, K., Kitamura, M. Sugimura, Y.（ Role： Joint author）

Amino Acids,  2009.4 

Animal Cell Technology 15:Characterization of highly reactive sequences for transglutaminase 2 and factor XIIIa

Sugimura Y., Kitamura M., Hosono H., Shibata, H., Maki M. and Hitomi K（ Role： Joint author）

Kluwar Academic Publishers  2008 

Animal Cell Technology 14: Immunological Analysis of Epidermal-type Transglutaminase (TGase 3) in Epithelium.

Kiyotaka Hitomi, Kanae Yamamoto, Koji Nishi, Yoshiaki Sugimura, and Masatoshi Maki（ Role： Joint author）

Kluwar Academic Publishers  2006 

トランスグルタミナーゼによる低分子量Gタンパク質の機能制御

和田文孝、人見清隆（ Role： Joint author）

化学と生物  2005.10 

Post translational modification of transglutaminase

（ Role： Sole author）

2005.6 

トランスグルタミナーゼの分子進化と多様性

人見清隆、和田文孝（ Role： Joint author）

化学と生物  2003.6 

皮膚表皮に存在するカルシウム依存性タンパク質架橋化酵素の発現と活性制御機構に関する研究

人見清隆（ Role： Sole author）

日本農芸化学会誌  2003.2 

Recent Research Developmemts in Biophysics and Biochemistry: Molecular Evolution of Transglutaminase: from bacteria to animal

Kiyotaka Hitomi（ Role： Sole author）

Research Signpost  2003 

Animal Cell Technology:Basic and Applied Aspects

"Hitomi, K., Ikura, K., and Maki, M.( EDITOR:Shirahata, S.)"（ Role： Joint author）

Kluwer Academic Publishers  2002 

Animal Cell Technology:Expression and Characterization of Mouse Epidermal Transglutaminase in Baculovirusinfected Insect Cells. (共著)

（ Role： Sole author）

1999 

Animal Cell Technology: Expression and characerization of human calpain and calpastatin

"Hitomi K. and Maki, M (EDITOR:Kitagawa Y.)"（ Role： Joint author）

1998 

Characterization of substrate peptide for skin TGs and applications for detection of activities and substrates International conference

Gordon Research Conference in Italy Transglutaminases in Human Disease Processes 

Identification and applications of the highly reactive substrate peptides for transglutaminases International conference

Gordon Research Conference 2012 

トランスグルタミナーゼが触媒するタンパク質のポリアミン修飾による生理機能の調節

人見清隆

日本農芸化学会大会シンポジウム 

タンパク質架橋化酵素トランスグルタミナーゼの高反応性基質配列の探索と多面的活用

人見清隆

酵素工学研究会第６６回講演会 

Identification of preferred substrate sequecens for tranglutaminase and utility for detection of activity in an isozyme-specific manner

Kiyotaka Hitomi

Identification of highly reactive substrate peptides for transglutaminases from random peptide library and their applications International conference

Gordon Research Conference: Trasnglutaminase in human diesease processes 

Identification and Application of Highly Reactive Substrate Peptides for Transglutaminases

Characterization of the preferred substrate sequence for transglutaminase, an enzyme for protein cross-linking and polyamine-conjugation International conference

Gordon Research Conference 2009 Polyamines 

タンパク質架橋酵素・トランスグルタミナーゼの反応機構解析と応用

日本農芸化学会２００８年度大会シンポジウム 

Identification and characterization of the preferred substrate sequences for transglutaminases International conference

Screening and identification of the preferred substrate sequences for transglutaminases using phage-displayed peptide library International conference

The 9th Transglutaminase and cross-linking proteins International Conference 

Screening and Identification of the Preferred Substrate Sequences of Factor XIII and Transglutmainase 2 International conference

The 51th annual meeting society of thrombosis and Haemostasis research/ Factor XIII symposium 

トランスグルタミナーゼの最小基質配列の解明とその活用

ポリアミンと核酸の共進化（第5回シンポジウム） 

Application of protein cross-linking enzyme, transglutamianse

トランスグルタミナーゼの傷害応答時の基質および基質認識機構の解析

人見清隆

第１回　ポリアミンとトランスグルタミナーゼ合同学術集会 

Analysis of transglutaminase substrates upon physical damage in slime mold International conference

The 8th Transglutaminase and cross-linking proteins International Conference 

Immunological analysis of epidermal-type transglutaminase (TGase 3) in epithelium

Japanese Animal and Cell Technology 

Characterization of Slime Mold Transglutaminase and Cross-linking in Wound Healing

人見清隆

第76回日本生化学会大会シンポジウム 

Molecular cloning and characterization of transglutaminase in slime mold-possible role for wound healing International conference

the 2nd Japan-Hungary Joint conference on Transglutaminase in Heviz 

皮膚表皮のトランスグルタミナーゼの活性制御機構とその応用

人見清隆

第4回酵素応用シンポジウム 

Molecular cloning and characterization of transglutaminase in slime mold (Physarum polycephalum) International conference

7th International Conference on Transglutaminase and protein cross-linking reactions 

皮膚表皮に存在するカルシウム依存性蛋白質架橋化酵素の発現と活性調節機構に関する研究

人見清隆

日本農芸化学会関東・中部支部合同例会受賞講演 

皮膚表皮におけるタンパク質架橋化酵素の解析と応用

2003.7 - 2005.6

武田科学振興財団（皮膚表皮の防御システム構築に関わるカルシウム依存性タンパク質架橋反応）

2003.6

武田科学振興財団一般研究助成 

国際交流助成（蛋白質架橋化酵素の皮膚表皮分化に伴う活性化機構に関する研究）

2001.6 - 2001.8

長瀬科学財団 

海外派遣助成(第6回トランスグルタミナーゼ国際会議参加補助）

2000.9

名古屋大学学術振興基金 

国際会議参加補助金

2000.9

農芸化学研究奨励金 

カルパインサブユニットファミリーの機能解析

1998.6 - 2002.1

医薬資源研究奨励金 

Molecular characterization of protein cross-linking reaction for cellular functions

2011.7 - 2014.3

Grant-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research(B)

Studies on utitlities of high reactivity substrate peptides for glue-peptide

2011.7 - 2013.3

Grant-in-Aid for Scientific Research 

Multiple application of cross-linking enzyme activity in life science

2008.11 - 2011.3

Grant-in-Aid for Scientific Research 

タンパク質架橋反応を担う酵素群の最小基質配列の解明と活用

2007.7 - 2009.3

科学研究費補助金  基盤研究（Ｃ）（一般），課題番号：19580103

人見　清隆

Molecular basis of formation of corneocyte in skin epidermis

2005.7 - 2007.2

Grant-in-Aid for Scientific Research 

高効率物質生産システムとしての浮遊-付着可変型動物細胞培養系の開発

1997.7 - 2000.2

科学研究費補助金  萌芽研究・09876023

人見清隆

皮膚表皮細胞内の架橋化反応を制御するカルシウム依存性機能蛋白質に関する研究

2002.7 - 2004.1

科学研究費補助金  基盤研究(C)(2)(一般)

人見

Analysis of activation mechanism of transglutaminase in skin epidermis

2000.7 - 2002.1

Grant-in-Aid for Scientific Research 

食品由来の骨形成調節因子の単離とその構造解析

1996.7 - 1997.2

科学研究費補助金  奨励研究（A)・08760131

アスコルビン酸-輸送担体に関する蛋白化学的研究

1993.7 - 1994.2

科学研究費補助金  奨励研究A05760107・

アスコルビン酸による骨形成効果の培養細胞系での解

1992.7 - 1993.2

科学研究費補助金  奨励研究A・04760061

Biotechnology

2011

細胞生物学３

2009

細胞生物学２

2009