Updated on 2024/04/02

写真a

 
KASHIDA, Hiromu
 
Organization
Graduate School of Engineering Biomolecular Engineering 1 Associate professor
Graduate School
Graduate School of Engineering
Undergraduate School
School of Engineering Chemistry and Biotechnology
Title
Associate professor
Contact information
メールアドレス

Degree 1

  1. 博士(工学) ( 2006.9   東京大学 ) 

Research Areas 1

  1. Others / Others  / Chemistry Related to Living Body

Research History 7

  1. PRESTO, JST

    2022.10 - 2026.3

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    Country:Japan

  2. Visiting Scholar, The Scripps Research Institute

    2019.7 - 2020.1

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    Country:Japan

  3. PRESTO, JST

    2014.10 - 2018.3

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    Country:Japan

  4. 名古屋大学大学院工学研究科准教授

    2013.2

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    Country:Japan

  5. 名古屋大学大学院工学研究科講師

    2011.5 - 2013.1

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    Country:Japan

  6. 名古屋大学大学院工学研究科助教

    2007.4 - 2011.4

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    Country:Japan

  7. 日本学術振興会特別研究員(PD)

    2006.10 - 2007.3

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    Country:Japan

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Education 3

  1. The University of Tokyo   Graduate School, Division of Engineering   Department of Chemistry and Biotechnology

    2004.4 - 2006.9

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    Country: Japan

  2. The University of Tokyo   Graduate School, Division of Engineering   Department of Chemistry and Biotechnology

    2002.4 - 2004.3

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    Country: Japan

  3. The University of Tokyo   Faculty of Engineering   Department of Chemistry and Biotechnology

    1998.4 - 2002.3

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    Country: Japan

Professional Memberships 4

  1. The Chemical Society of Japan

  2. The Society of Polymer Science, Japan

  3. 光化学協会

  4. 核酸化学会

Committee Memberships 5

  1. 高分子学会東海支部 東海高分子研究会   代表幹事  

    2020.4 - 2021.3   

  2. 高分子学会東海支部 東海高分子研究会   庶務幹事  

    2016.4 - 2017.3   

  3. 高分子学会 高分子編集委員会   委員  

    2015.6 - 2017.5   

  4. 化学グランプリ   二次選考運営委員  

    2015 - 2016   

  5. 日本化学会生体機能関連化学部会   若手代表幹事  

    2013.4 - 2014.3   

Awards 10

  1. SPSJ Science Award 2022

    2023.5   The Society of Polymer Science, Japan   Development of Supramolecular Materials Based on Artificial Nucleic Acids

    Hiromu Kashida

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    Award type:Award from Japanese society, conference, symposium, etc.  Country:Japan

  2. The Commendation for Science and Technology by the Minister of Education, Culture, Sports, Science and Technology: The Young Scientists' Prize

    2017.4   Ministry of Education, Culture, Sports, Science and Technology  

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    Country:Japan

  3. Award for Encouragement of Research in Polymer Science

    2015.5   The Society of Polymer Science, Japan  

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    Country:Japan

  4. The Chemical Society of Japan Award for Young Chemists

    2014.3   The Chemical Society of Japan  

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    Country:Japan

  5. Outstanding Oral Presentation Award

    2013.11   The 40th International Symposium on Nucleic Acids Chemistry  

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    Country:Japan

  6. 赤崎賞

    2012.3   名古屋大学  

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    Country:Japan

  7. 講演賞

    2011.9   第5回バイオ関連化学シンポジウム  

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    Country:Japan

  8. 若手研究者奨励講演賞

    2011.7   第21回バイオ高分子研究会シンポジウム  

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    Country:Japan

  9. Best Oral Presentation by a Young Scientist

    2011.6   XVth Symposium on Chemistry of Nucleic Acid Components  

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    Country:Czech Republic

  10. PCCP Prize

    2011.3   Royal Society of Chemistry, PCCP (Physical Chemistry Chemical Physics) and Faraday Discussion  

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    Country:Japan

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Papers 101

  1. Photosensitization of TiO2 electrodes immobilized with chiral plasmonic Au nanocolloids by circularly polarized light irradiation Invited Reviewed

    Tatsuya Kameyama, Shuji Isogawa, Kazutaka Akiyoshi, Hiromu Kashida, Hiroyuki Asanuma, Tsukasa Torimoto

    JOURNAL OF CHEMICAL PHYSICS   Vol. 160 ( 9 ) page: 094709   2024.3

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1063/5.0192366

  2. Site-Selective Photo-Crosslinking of Stilbene Pairs in a DNA Duplex Mediated by Ruthenium Photocatalyst Reviewed

    Kashida, H; Azuma, H; Sotome, H; Miyasaka, H; Asanuma, H

    ANGEWANDTE CHEMIE-INTERNATIONAL EDITION   Vol. 63 ( 14 ) page: e202319516   2024.2

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    Authorship:Lead author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1002/anie.202319516

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  3. Orientational Control of Circularly Polarized Luminescence from Pyrene Clusters by Using a DNA Scaffold Reviewed

    Kashida Hiromu, Ito Yuka, Kakuta Takahiro, Ogoshi Tomoki, Asanuma Hiroyuki

    CHEMISTRY-A EUROPEAN JOURNAL   Vol. 29 ( 22 ) page: e202300182   2023.4

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    Authorship:Lead author, Corresponding author   Language:English  

    DOI: 10.1002/chem.202300182

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  4. Methyl group configuration on acyclic threoninol nucleic acids (aTNAs) impacts supramolecular properties Reviewed

    Murayama Keiji, Kashida Hiromu, Asanuma Hiroyuki

    ORGANIC & BIOMOLECULAR CHEMISTRY   Vol. 20 ( 20 ) page: 4115 - 4122   2022.5

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1039/d2ob00266c

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  5. Xeno nucleic acids (XNAs) having non-ribose scaffolds with unique supramolecular properties Reviewed

    Asanuma Hiroyuki, Kamiya Yukiko, Kashida Hiromu, Murayama Keiji

    CHEMICAL COMMUNICATIONS   Vol. 58 ( 25 ) page: 3993 - 4004   2022.3

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1039/d1cc05868a

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  6. Color-Changing Fluorescent Barcode Based on Strand Displacement Reaction Enables Simple Multiplexed Labeling Reviewed

    Makino Koki, Susaki Etsuo A., Endo Motomu, Asanuma Hiroyuki, Kashida Hiromu

    JOURNAL OF THE AMERICAN CHEMICAL SOCIETY   Vol. 144 ( 4 ) page: 1572 - 1579   2022.2

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1021/jacs.1c09844

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  7. Pseudo Base Pairs that Exhibit High Duplex Stability and Orthogonality through Covalent and Non-covalent Interactions Invited Reviewed

    Kashida Hiromu, Asanuma Hiroyuki

    JOURNAL OF SYNTHETIC ORGANIC CHEMISTRY JAPAN   Vol. 79 ( 11 ) page: 1013 - 1019   2021.11

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  8. A Pyrene-Modified Serinol Nucleic Acid Nanostructure Converts the Chirality of Threoninol Nucleic Acids into Circularly Polarized Luminescence Signals Reviewed

    Kashida Hiromu, Nishikawa Keiji, Ito Yuka, Murayama Keiji, Hayashi Ichiyo, Kakuta Takahiro, Ogoshi Tomoki, Asanuma Hiroyuki

    CHEMISTRY-A EUROPEAN JOURNAL   Vol. 27 ( 59 ) page: 14582 - 14585   2021.10

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  9. Perylene-Cy3 FRET System to Analyze Photoactive DNA Structures Reviewed

    Kawai Hayato, Doi Tetsuya, Ito Yuka, Kameyama Tatsuya, Torimoto Tsukasa, Kashida Hiromu, Asanuma Hiroyuki

    CHEMISTRY-A EUROPEAN JOURNAL   Vol. 27 ( 50 ) page: 12845 - 12850   2021.9

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  10. Microheater-integrated zinc oxide nanowire microfluidic device for hybridization-based detection of target single-stranded DNA Reviewed

    Takahashi Hiromi, Yasui Takao, Kashida Hiromu, Makino Koki, Shinjo Keiko, Liu Quanli, Shimada Taisuke, Rahong Sakon, Kaji Noritada, Asanuma Hiroyuki, Baba Yoshinobu

    NANOTECHNOLOGY   Vol. 32 ( 25 )   2021.6

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  11. A helical amplification system composed of artificial nucleic acids Reviewed

    Kashida Hiromu, Nishikawa Keiji, Shi Wenjing, Miyagawa Toshiki, Yamashita Hayato, Abe Masayuki, Asanuma Hiroyuki

    CHEMICAL SCIENCE   Vol. 12 ( 5 )   2021.2

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    Authorship:Lead author, Corresponding author   Language:English  

    DOI: 10.1039/d0sc05245k

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  12. Quantitative Analyses of Forster Resonance Energy Transfer between Identical Pyrene Chromophores (Homo-FRET) In DNA Scaffolds. Reviewed

    Kashida Hiromu, Kawai Hayato, Azuma Hidenori, Araki Yasuyuki, Wada Takehiko, Asanuma Hiroyuki

    CHEMPHOTOCHEM   Vol. 5 ( 2 ) page: 167 - 172   2021.2

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1002/cptc.202000199

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  13. Preparation of artificial hexaplex composed of non-natural nucleic acid Reviewed

    Koki Makino, Yuhei Hattori, Hiromu Kashida, Hiroyuki Asanuma

    CURRENT PROTOCOLS     2021

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    Authorship:Corresponding author   Language:English  

    DOI: 10.1002/cpz1.106

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  14. Intrastrand backbone-nucleobase interactions stabilize unwound right-handed helical structures of heteroduplexes of L-<i>a</i>TNA/RNA and SNA/RNA Reviewed

    Kamiya, Y; Satoh, T; Kodama, A; Suzuki, T; Murayama, K; Kashida, H; Uchiyama, S; Kato, K; Asanuma, H

    COMMUNICATIONS CHEMISTRY   Vol. 3 ( 1 )   2020.11

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  15. Efficient Light-Harvesting Antennae Resulting from the Dense Organization of Dyes into DNA Junctions though d-Threoninol Reviewed

    Kashida Hiromu, Azuma Hidenori, Maruyama Ryoko, Araki Yasuyuki, Wada Takehiko, Asanuma Hiroyuki

    ANGEWANDTE CHEMIE-INTERNATIONAL EDITION   Vol. 59 ( 28 ) page: 11360 - 11363   2020.7

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    Language:Japanese   Publishing type:Research paper (scientific journal)  

    DOI: 10.1002/anie.202004221

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  16. A triplex-forming linear probe for sequence-specific detection of duplex DNA with high sensitivity and affinity Reviewed

    Chen Yanglingzhi, Murayama Keiji, Kashida Hiromu, Kamiya Yukiko, Asanuma Hiroyuki

    CHEMICAL COMMUNICATIONS   Vol. 56 ( 40 ) page: 5358 - 5361   2020.5

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    Language:Japanese   Publishing type:Research paper (scientific journal)  

    DOI: 10.1039/d0cc01865a

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  17. Nanometer Accuracy in Cryogenic Far-Field Localization Microscopy of Individual Molecules Reviewed

    Furubayashi Taku, Ishida Keita, Kashida Hiromu, Nakata Eiji, Morii Takashi, Matsushita Michio, Fujiyoshi Satoru

    JOURNAL OF PHYSICAL CHEMISTRY LETTERS   Vol. 10 ( 19 ) page: 5841 - 5846   2019.10

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1021/acs.jpclett.9b02184

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  18. Selective binding of nucleosides to gapped DNA duplex revealed by orientation and distance dependence of FRET Reviewed

    Kashida Hiromu, Kokubo Yuta, Makino Koki, Asanuma Hiroyuki

    ORGANIC & BIOMOLECULAR CHEMISTRY   Vol. 17 ( 28 ) page: 6786 - 6789   2019.7

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1039/c9ob00946a

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  19. N-Benzoyl-protected Peptide Nucleic Acid (PNA) Monomers Expand the Range of Nucleobases Available for PNA-DNA Chimera Reviewed

    Inagaki Masahito, Uematsu Ryohei, Mizutani Tatsuya, Unabara Daisuke, Araki Yasuyuki, Sakamoto Seiji, Kashida Hiromu, Nishijima Masaki, Asanuma Hiroyuki, Inoue Yoshihisa, Wada Takehiko

    CHEMISTRY LETTERS   Vol. 48 ( 4 ) page: 341 - 344   2019.4

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    Language:Japanese   Publishing type:Research paper (scientific journal)  

    DOI: 10.1246/cl.181048

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  20. In-stem molecular beacon targeted to a 5 '-region of tRNA inclusive of the D arm that detects mature tRNA with high sensitivity Reviewed

    Miyoshi Yuichi, Ohtsuki Takashi, Kashida Hiromu, Asanuma Hiroyuki, Watanabe Kazunori

    PLOS ONE   Vol. 14 ( 1 )   2019.1

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1371/journal.pone.0211505

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  21. Photo-regulatable DNA isothermal amplification by template-mediated ligation Reviewed

    Cheng Bohao, Kashida Hiromu, Shimada Naohiko, Maruyama Atsushi, Asanuma Hiroyuki

    CHEMICAL COMMUNICATIONS   Vol. 55 ( 8 ) page: 1080 - 1083   2019.1

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1039/c8cc09218d

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  22. The Use of Serinol Nucleic Acids as Ultrasensitive Molecular Beacons

    Murayama Keiji, Kashida Hiromu, Asanuma Hiroyuki

    NON-NATURAL NUCLEIC ACIDS: METHODS AND PROTOCOLS   Vol. 1973   page: 261 - 279   2019

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1007/978-1-4939-9216-4_17

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  23. Quantitative evaluation of energy migration between identical chromophores enabled by breaking symmetry Reviewed

    Kashida Hiromu, Kawai Hayato, Maruyama Ryoko, Kokubo Yuta, Araki Yasuyuki, Wada Takehiko, Asanuma Hiroyuki

    COMMUNICATIONS CHEMISTRY   Vol. 1   2018.12

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1038/s42004-018-0093-0

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  24. The DNA Duplex as an Aqueous One-Dimensional Soft Crystal Scaffold for Photochemistry Invited Reviewed

    Asanuma Hiroyuki, Murayama Keiji, Kamiya Yukiko, Kashida Hiromu

    BULLETIN OF THE CHEMICAL SOCIETY OF JAPAN   Vol. 91 ( 12 ) page: 1739 - 1748   2018.12

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    Language:English  

    DOI: 10.1246/bcsj.20180278

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  25. Bifacial nucleobases for hexaplex formation in aqueous solution Reviewed

    H. Kashida, Y. Hattori, K. Tazoe, T. Inoue, K. Nishikawa, K. Ishii, S. Uchiyama, H. Yamashita, M. Abe, Y. Kamiya, H. Asanuma

    J. Am. Chem. Soc.   Vol. 140 ( 27 ) page: 8456 - 8462   2018.7

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    Language:Japanese   Publishing type:Research paper (scientific journal)  

    DOI: 10.1021/jacs.8b02807

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  26. Chaperone polymer-assisted, photo-driven DNA strand displacement Reviewed

    B. Cheng, H. Kashida, N. Shimada, A. Maruyama, H. Asanuma

    ChemBioChem   Vol. 18 ( 16 ) page: 1568 - 1572   2017.8

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    Language:Japanese   Publishing type:Research paper (scientific journal)  

    DOI: 10.1002/cbic.201700202

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  27. Orientation-dependent FRET system reveals differences in structures and flexibilities of nicked and gapped DNA duplexes Reviewed

    H. Kashida, A. Kurihara, H. Kawai, H. Asanuma

    Nucleic Acids Res.   Vol. 45 ( 11 ) page: e105   2017.6

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    Language:Japanese   Publishing type:Research paper (scientific journal)  

    DOI: 10.1093/nar/gkx200

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  28. Development of Pseudo Base-Pairs on d-Threoninol which Exhibit Various Functions Invited Reviewed

    H. Kashida, H. Asanuma

    Bull. Chem. Soc. Jpn.   Vol. 90 ( 5 ) page: 475 - 484   2017.5

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    DOI: 10.1246/bcsj.20160371

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  29. Design of photofunctional oligonucleotides by copolymerization of natural nucleobases with base surrogates prepared from acyclic scaffolds Invited Reviewed

    H. Asanuma, K. Murayama, Y. Kamiya, H. Kashida

    Polymer J.   Vol. 49 ( 3 ) page: 279 - 289   2017.3

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    Language:Japanese  

    DOI: 10.1038/pj.2016.120

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  30. DNA二重鎖による色素会合体の精密位置配向制御と光化学への展開 Invited

    浅沼浩之、樫田啓

    固体物理   Vol. 52 ( 3 ) page: 149-158   2017

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    Language:Japanese  

  31. Development of Pseudo Base Pairs Which Show High DNA Duplex Stabilities and Orthogonality Invited Reviewed

    Kashida, H; Asanuma, H

    KOBUNSHI RONBUNSHU   Vol. 74 ( 4 ) page: 257 - 264   2017

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    Language:Japanese  

    DOI: 10.1295/koron.2017-0009

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  32. Strand-invading linear probe combined with unmodified PNA Reviewed

    H. Asanuma, R. Niwa, M. Akahane, K. Murayama, H. Kashida, Y. Kamiya

    Bioorg. Med. Chem.   Vol. 24   page: 4129-4137   2016.9

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.bmc.2016.06.055

  33. Faint electric treatment-induced rapid endosomal escape for efficient delivery of functional macromolecules into the cytoplasm Reviewed

    M. Hasan, A. Nishimoto, T. Ohgita, S. Hama, H. Kashida, H. Asanuma, K. Kogure

    J. Control Release   Vol. 228   page: 20-25   2016

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  34. Acyclic artificial nucleic acids with phosphodiester bonds exhibit unique functions" Reviewed

    H. Kashida, K. Murayama, H. Asanuma

    Polymer J.   Vol. 48   page: 781-786   2016

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  35. Visible Light-Triggered Crosslinking of DNA Duplex by Reversible [2+2] Photocycloaddition of Styrylpyrene Reviewed

    T. Doi, H. Kawai, K. Murayama, H. Kashida, H. Asanuma

    Chem. Eur. J.   Vol. 22   page: 10533-10538   2016

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  36. A stem-less probe using spontaneous pairing between Cy3 and quencher for RNA detection Reviewed

    H. Kashida, K. Morimoto, H. Asanuma

    Sci. Technol. Adv. Mater.   Vol. 17   page: 267-273   2016

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  37. 新規人工核酸SNAを用いたRNAイメージング Invited

    樫田啓、村山恵司、浅沼浩之

    生体の科学   Vol. 66 ( 2 ) page: 145-150   2015

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  38. Ultrasensitive Molecular Beacon Designed with Totally Serinol Nucleic Acid (SNA) for Monitoring mRNA in Cells Reviewed

    K. Murayama, Y. Kamiya, H. Kashida, H. Asanuma

    ChemBioChem   Vol. 16 ( 9 ) page: 1298-1301   2015

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  39. Efficiency of [2+2] photodimerization of various stilbene derivatives within the DNA duplex scaffold Reviewed

    T. Doi, H. Kashida, H. Asanuma

    Org. Biomol. Chem.   Vol. 13 ( 15 ) page: 4430-4437   2015

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  40. Acyclic L-threoninol nucleic acid (L-aTNA) with suitable structural rigidity cross-pairs with DNA and RNA Reviewed

    K. Murayama, H. Kashida, H. Asanuma

    Chem. Commun.   Vol. 51 ( 30 ) page: 6500-6503   2015

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  41. Molecular design of Cy3 derivative for highly sensitive in-stem molecular beacon and its application to the wash-free FISH Reviewed

    H. Kashida, T. Osawa, K. Morimoto, Y. Kamiya, H. Asanuma

    Bioorg. Med. Chem.   Vol. 23 ( 8 ) page: 1758-1762   2015

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  42. Highly Sensitive and Robust Linear Probe for Detection of mRNA in Cells Reviewed

    H. Asanuma, M. Akahane, R. Niwa, H. Kashida, Y. Kamiya

    Angew. Chem. Int. Ed.   Vol. 54 ( 14 ) page: 4315-4319   2015

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  43. Hetero-Selective DNA-like Duplex Stabilized by Donor-Acceptor Interaction Reviewed

    T. Doi, T. Sakibara, H. Kashida, Y. Araki, T. Wada, H. Asanuma

    Chem. Eur. J.   Vol. 21   page: 15974-15980   2015

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  44. Terminus-free siRNA prepared by photo-crosslinking activated via slicing by Ago2 Reviewed

    Y. Kamiya, K. Iishiba, T. Doi, K. Tsuda, H. Kashida, H. Asanuma

    Biomater. Sci.   Vol. 3   page: 1534-1538   2015

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  45. DNA二重鎖を足場とした配向依存的FRET Invited

    浅沼浩之、栗原綾子、樫田啓

    光化学   Vol. 45   page: 146-147   2014

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  46. De Novo Design of Functional Oligonucleotides with Acyclic Scaffolds Reviewed

    H. Asanuma, H. Kashida, Y. Kamiya

    Chem. Rec.   Vol. 14 ( 6 ) page: 1055-1069   2014

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  47. Enhancement of Stability and Activity of siRNA by Terminal Substitution with Serinol Nucleic Acid (SNA) Reviewed

    Y. Kamiya, J. Takai, H. Ito, K. Murayama, H. Kashida, H. Asanuma

    ChemBioChem   Vol. 15 ( 17 ) page: 2549-2555   2014

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  48. Development of a robust model system of FRET using base-surrogates tethering fluorophores for strict control of their position and orientation within DNA duplex Reviewed

    T. Kato, H. Kashida, H. Kishida, H. Yada, H. Okamoto, H. Asanuma

    J. Am. Chem. Soc.   Vol. 135   page: 741-750   2013

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  49. Selective labeling of mature RISC using siRNA carrying fluorophore-quencher pair Invited Reviewed

    Y. Kamiya, A. Ito, H. Ito, M. Urushihara, J. Takai, T. Fujii, X.G. Liang, H. Kashida, H. Asanuma

    Chem. Sci.   Vol. 4   page: 4016-4021   2013

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  50. The "sugar-deficient" G-quadruplex: incorporation of aTNA in quadruplex structures Invited Reviewed

    J. Zhou, K. Murayama, S. Amrane, H. Kashida, A. Bourdoncle, H. Asanuma, J. L. Mergny

    Chem. Sci.   Vol. 4   page: 3693-3698   2013

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  51. Highly stable duplex formation by artificial nucleic acids aTNA and SNA with acyclic scaffolds Invited Reviewed

    K. Murayama, Y. Tanaka, T. Toda, H. Kashida, H. Asanuma

    Chem. Eur. J.   Vol. 19   page: 14151-14158   2013

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

  52. p-Stilbazole moieties as artificial base pairs for photocrosslinking of DNA duplex Reviewed

    H. Kashida, T. Doi, T. Sakakibara, T. Hayashi, H. Asanuma

    J. Am. Chem. Soc.   Vol. 135   page: 7960-7966   2013

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  53. Evaluation of intrinsic spectroscopic properties of chromophore assemblies by shielding with cyclohexyl base pairs within a DNA duplex Invited Reviewed

    H. Kashida, N. Higashiyama, T. Kato, H. Asanuma

    Bioorg. Med. Chem.   Vol. 21 ( 20 ) page: 6191-6197   2013

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

  54. Highly Stable Duplex Formation by Artificial Nucleic Acids Acyclic Threoninol Nucleic Acid (aTNA) and Serinol Nucleic Acid (SNA) with Acyclic Scaffolds Reviewed

    K. Murayama, Y. Tanaka, T. Toda, H. Kashida, H. Asanuma

    Chem. Eur. J.   Vol. 19 ( 42 ) page: 14151-14158   2013

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  55. SNA:配列設計でらせん構造が制御可能な核酸アナログ

    浅沼浩之、村山恵司、樫田啓

    高分子   Vol. 62   page: 513-514   2013

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    Language:Japanese  

  56. Design of an Artificial Functional Nanomaterial with High Recognition Ability Reviewed

    X.G. Liang, T. Mochizuki, T. Fujii, H. Kashida, H. Asanuma

    Natural Computing   Vol. 11   page: 231-238   2012

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  57. Reversed assembling of dyes in RNA duplex compared with those in DNA Reviewed

    T. Fujii, M. Urushihara, H. Kashida, H. Ito, X.G. Liang, M. Yagi-Utsumi, K. Kato, H. Asanuma

    Chem. Eur. J.   Vol. 18   page: 13304-13313   2012

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  58. Bulge-like asymmetric hetero dye-clustering in DNA duplex results in efficient quenching of background emission based on the maximized excitonic interaction Reviewed

    T. Fujii, Y. Hara, T. Osawa, H. Kashida, X.G. Liang, Y. Yoshida, H. Asanuma

    Chem. Eur. J.   Vol. 18   page: 10865-10872   2012

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  59. Quencher-free molecular beacon tethering 7-hydroxycoumarin detects targets through protonation/deprotonation Reviewed

    H. Kashida, K. Yamaguchi, Y. Hara, H. Asanuma

    Bioorg. Med. Chem.   Vol. 20   page: 4310-4315   2012

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  60. Preparation of supramolecular chromophoric assemblies using a DNA duplex Reviewed

    H. Kashida, H. Asanuma

    Phys. Chem. Chem. Phys.   Vol. 14   page: 7196-7204   2012

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  61. Quencher-free linear probe with multiple fluorophores on acyclic scaffold Reviewed

    H. Asanuma, M. Akahane, N. Kondo, T. Osawa, T. Kato, H. Kashida

    Chem. Sci.     page: accepted.   2012

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  62. Coherent Interactions of Dyes Assembled on DNA Invited Reviewed

    H. Asanuma, T. Fujii, T. Kato, H. Kashida

    J. Photochem. Photobiol. C   Vol. 13   page: 1065-1084   2012

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  63. Polycation-chaperoned in-stem molecular beacon system Reviewed

    H. Asanuma, T. Osawa, H. Kashida, T. Fujii, X.G. Liang, K. Niwa, Y. Yoshida, N. Shimada, A. Maruyama

    Chem. Commun.   Vol. 48   page: 1760-1762   2012

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  64. Design of a Functional Nanomaterial with Recognition Ability for Constructing Light-Driven Nanodevices Reviewed

    X. G. Liang, T. Mochizuki, T. Fujii, H. Kashida, H. Asanuma

    LCNS   Vol. 6518   page: 112-122   2011

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  65. Detection of three-base deletion by exciplex formation with perylene derivatives Reviewed

    H. Kashida, N. Kondo, K. Sekiguchi, H. Asanuma

    Chem. Commun.   Vol. 47   page: 6404-6406   2011

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  66. Cationic dye-triplet as unique glue that can connect full-matched termini of the duplexes Reviewed

    H. Kashida, T. Hayashi, T. Fujii, H. Asanuma

    Chem. Eur. J.   Vol. 17   page: 2614-2622   2011

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  67. Control of the Chirality and Helicity of Oligomers of Serinol Nucleic Acid (SNA) by Sequence Design." Reviewed

    H. Kashida, K. Murayama, T. Toda, H. Asanuma

    Angew. Chem. Int. Ed.   Vol. 50   page: 1285-1288   2011

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  68. Cyclohexyl base pairs stabilize duplexes and intensify pyrene fluorescence by shielding it from natural base pairs Reviewed

    H. Kashida, K. Sekiguchi, N. Higashiyama, T. Kato, H. Asanuma

    Org. Biomol. Chem.   Vol. 9   page: 8313-8320   2011

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    DOI: 10.1039/c1ob06325a

  69. Insulator base pairs for lighting-up perylenediimide in a DNA duplex. Reviewed

    H. Kashida, K. Sekiguchi, H. Asanuma.

    Chem. Eur. J.   Vol. 16   page: 11554-11557   2010

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  70. Coherent Quenching of a Fluorophore for the Design of a Highly Sensitive In-Stem Molecular Beacon Reviewed

    Y. Hara, T. Fujii, H. Kashida, K. Sekiguchi, X. G. Liang, K. Niwa, T. Takase, Y. Yoshida, H. Asanuma.

    Angew. Chem. Int. Ed.   Vol. 49   page: 5502-5506   2010

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  71. Accumulation of fluorophores into DNA duplexes to mimic the properties of quantum dots Reviewed

    J. Am. Chem. Soc.   Vol. 132   page: 6223-6230   2010

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  72. An efficient FRET between pyrene and perylene assembled in a DNA duplex and its potential for discriminating single base changes. Reviewed

    H. Kashida, T. Takatsu, K. Sekiguchi, H. Asanuma

    Chem. Eur. J.   Vol. 16   page: 2479-2486   2010

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    Fluorescence resonance energy transfer between the donor (pyrene) and acceptor (perylene) was observed within the DNA double helix. In our design, chromophores were arranged in a pre-determined order and position. Furthermore, a one-base deletion was successfully detected with a large apparent Stokes' shift.

  73. Unexpectedly stable artificial duplex from flexible acyclic threoninol Reviewed

    H. Asanuma, T. Toda, K. Murayama, X. G. Liang, H. Kashida

    J. Am. Chem. Soc.   Vol. 132   page: 14702-14703   2010

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  74. Rational design of functional DNA with a non-ribose acyclic scaffold Reviewed

    H. Kashida, X. G. Liang, H. Asanuma

    Curr. Org. Chem.   Vol. 13   page: 1065-1084   2009.7

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  75. Modulation of pKa of Brooker's Merocyanine by DNA Hybridization Reviewed

    H. Kashida, K. Sano, Y. Hara, H. Asanuma.

    Bioconjugate Chem.   Vol. 20   page: 258-265   2009.2

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    Brooker's merocyanine (BM), which changes its emission and absorption maxima upon protonation, was introduced into ODN via D-threoninol. The pK(a) of BM in the modified ODN increased from 9.5 to 10.1 upon hybridization. Ratiometric detection of hybridization was also possible by simultaneous excitation of both protonated and deprotonated BMs. Furthermore, we could also modulate its pK(a) by the antiparallel stacking of two BM molecules in the duplex. Thus, control of the microenvironment around the BM molecule allowed modulation of its pK(a), which is applicable to the sequence-specific recognition of target DNA.

  76. *Positively charged base surrogate for highly stable “base-pairing" through electrostatic and stacking interactions Reviewed

    H. Kashida, H. Ito, T. Fujii, T. Hayashi, H. Asanuma

    J. Am. Chem. Soc.   Vol. 131   page: 9928-9930   2009

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    "Base pairs" of cationic dyes (p-methylstilbazole) were incorporated into oligodeoxyribonucleotides (ODNs). This "base pair" greatly stabilized the duplex through electrostatic and stacking interactions. The melting temperature of modified ODN was higher than those of neutral dyes and native base pairs. Further stabilization of the duplex was observed when the number of cationic dyes increased.

  77. Analysis of Coherent Heteroclustering of Different Dyes by Use of Threoninol-Nucleotides for Comparison with the Molecular Exciton Theory Reviewed

    T. Fujii, H. Kashida, H. Asanuma

    Chem. Eur. J.   Vol. 15   page: 10092-10102   2009

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  78. *In-Stem Molecular Beacon containing a Pseudo Base Pair of Threoninol Nucleotides for Removal of Background Emission. Reviewed

    H. Kashida, T. Takatsu, T. Fujii, K. Sekiguchi, X. G. Liang, K. Niwa, T. Takase, Y. Yoshida, H. Asanuma.

    Angew. Chem. Int. Ed.   Vol. 48   page: 7044-7047   2009

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    An in-stem molecular beacon (IS-MB), which tethers perylene and anthraquinone in the stem region via D-threoninol, effectively detected target sequences. This design of IS-MB is also applicable to the discrimination of a one-base deletion mutant from wild type (full-match) without background emission.

  79. 第3の塩基対をつくる

    樫田啓

    化学   Vol. 11   page: 70-71   2009

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  80. Efficient energy transfer from pyrene to perylene assembled inside DNA duplex

    H. Kashida, T. Takatsu, H. Asanuma

    Nucleic Acids Symp. Ser.   Vol. 53   page: 29-30   2009

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  81. Postsynthetic modification of DNA via threoninol on a solid support by means of allylic protection. Reviewed

    H. Asanuma, Y. Hara, A. Noguchi, K. Sano, H. Kashida

    Tetrahedron Lett.   Vol. 49   page: 5144-5146   2008.7

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  82. Threoninol as a Scaffold of Dyes (Threoninol-nucleotide) and Their Stable Interstrand Clustering in Duplexes. Reviewed

    H. Kashida, T. Fujii, H. Asanuma

    Org. Biomol. Chem.   Vol. 6   page: 2892-2899   2008.6

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    Functional molecules such as dyes (Methyl Red, azobenzene, and Naphthyl Red) were tethered on D-threoninol as base surrogates (threoninol-nucleotide), which were consecutively incorporated at the center of natural oligodeoxyribonucleoticles (ODNs). Hybridization of two ODNs involving threoninol-nucleotides allowed interstrand clustering of the dyes on D-threoninol and greatly stabilized the duplex. Clustering of Methyl Reds induced both hypsochromicity and narrowing of the band, demonstrating that Methyl Reds were axially stacked relative to each other (H-aggregation). Alternate hetero dye clusters could also be prepared only by hybridization of two ODNs with different threoninol-nucleotides, such as Methyl Red-azobenzene and Methyl Red-Naphthyl Red combinations. Thus, D-threoninol can be a versatile scaffold for introducing functional molecules into DNA for their ordered clustering.

  83. Preparation of coherent hetero clusters with threoninol scaffold

    T. Fujii, H. Kashida, H. Asanuma

    Nucleic Acids Symp. Ser.   Vol. 52   page: 699-700   2008

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  84. Incorporation of cationic dyes into DNA for distinct stabilization of duplex

    H. Kashida, H. Itoh, T. Fujii, H. Asanuma.

    Nucleic Acids Symp. Ser.   Vol. 52   page: 701-702   2008

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  85. Detection of genetic polymorphisms with high sensitivityby DNA–perylene conjugate Reviewed

    H. Kashida, T. Takatsu, H. Asanuma

    Tetrahedron Lett.   Vol. 48   page: 6759-6762   2007.7

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  86. Development of high-sensitive DNA probe by using perylene.

    H. Kashida, T. Takatsu, H. Asanuma

    Nucleic Acids Symp. Ser.   Vol. 51   page: 279-280   2007

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  87. Preparation of "comb-type" hetero aggregates by DNA-dye conjugation.

    T. Fujii, H. Kashida, H. Asanuma

    Nucleic Acids Symp. Ser.   Vol. 51   page: 277-278   2007

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  88. Construction of novel dye aggregates based on "comb-type" sequence.

    H. Kashida, T. Fujii, H. Asanuma

    Nucleic Acids Symp. Ser.   Vol. 51   page: 11-12   2007

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  89. 2',6'-Dimethylazobenzene as an efficient and thermo-stable photo-regulator for the photoregulation of DNA hybridization. Reviewed

    H. Nishioka, X. G. Liang, H. Kashida, H. Asanuma

    Chem. Commun.     page: 4354-4356   2007

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  90. Exciplex formation between pyrene and N,N-dimethylaniline in DNA for the detection of one-base deletion Reviewed

    H. Kashida, M. Komiyama, H. Asanuma

    Chem. Lett.   Vol. 35 ( 8 ) page: 934-035   2006.8

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  91. Covalent incorporation of methyl red dyes into double-stranded DNA for their ordered clustering Reviewed

    H. Kashida, M. Tanaka, S. Baba, T. Sakamoto, G. Kawai, H. Asanuma, M. Komiyama

    Chem. --Eur. J.   Vol. 12 ( 3 ) page: 777-784   2006.1

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  92. Insertion of two pyrene moieties into oligodeoxyribonucleotides for the efficient detection of deletion polymorphisms Reviewed

    H. Kashida, H. Asanuma, M. Komiyama

    Chem. Commun.   ( 26 ) page: 2768-2770   2006.1

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    For the detection of deletion polymorphisms, two pyrene moieties are tethered to oligodeoxyribonucleotide (ODN) on both sides of the intervening base. When this probe ODN was hybridized with wild type ODN, both pyrenes intercalated between base pairs and only monomer emission was observed. In contrast, strong excimer emission was generated by hybridization with one-base deletion mutant. Similarly, two-base deletion was also detected.

  93. Enhancement of RNA cleavage activity of 10-23 DNAzyme by covalently introduced intercalator Reviewed

    H. Asanuma, H. Hayashi, J. Zhao, X. G. Liang, A. Yamazawa, T. Kuramochi, D. Matsunaga, Y. Aiba, H. Kashida, M. Komiyama

    Chem. Commun.   ( 48 ) page: 5062-5064   2006

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  94. Incorporation of methyl group on azobenzene for the effective photo-regulation of hybridization and suppression of thermal isomerization

    H. Nishioka, H. Kashida, M. Komiyama, X. G. Liang, H. Asanuma

    Nucleic Acids Symp. Ser.   Vol. 50   page: 85-86   2006

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  95. Interstrand H-aggregation of cationic dyes for narrowing the absorption spectra and stabilizing the duplex Reviewed

    H. Kashida, H. Asanuma, M. Komiyama

    Supramol. Chem.   Vol. 16 ( 6 ) page: 459-464   2004.9

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  96. Alternating hetero H aggregation of different dyes by interstrand stacking from two DNA-dye conjugates

    H. Kashida, H. Asanuma, M. Komiyama

    Angew. Chem. Int. Ed.   Vol. 43 ( 47 ) page: 6522-6525   2004

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    Heteroaggregates in which two dyes are stacked alternately can be prepared by interstrand stacking from two DNA–dye conjugates. Even different dyes can exhibit an H band as a result of aggregation. The present method is applicable to other functional molecules, and various novel materials can be produced by the attachment of dyes to DNA.

  97. 光機能性DNAの設計と応用

    浅沼浩之、樫田啓

    高分子加工   Vol. 53 ( 1 ) page: 37-43   2004

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  98. NMR study on the photoresponsive DNA tethering an azobenzene. Assignment of the absolute configuration of two diastereomers and structure determination of their duplexes in the trans-form Reviewed

    X. G. Liang, H. Asanuma, H. Kashida, A. Takasu, T. Sakamoto, G. Kawai, M. Komiyama

    J. Am. Chem. Soc.   Vol. 125 ( 52 ) page: 16408-16415   2003.12

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  99. DNA-dye conjugates for controllable H* aggregation Reviewed

    H. Asanuma, K. Shirasuka, T. Takarada, H. Kashida, M. Komiyama

    J. Am. Chem. Soc.   Vol. 125 ( 8 ) page: 2217-2223   2003.12

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  100. DNA-Naphthyl Red conjugate as a visualizing probe of DNA hybridization Reviewed

    H. Asanuma, H. Kashida, X. G. Liang, M. Komiyama

    Chem. Commun.   ( 13 ) page: 1536-1537   2003

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  101. Development of a probe DNA which accompanies color change on hybridization

    H. Kashida, H. Asanuma, M. Komiyama

    Nucleic Acids Res. Suppl.   Vol. 3   page: 143-144   2003

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▼display all

Books 5

  1. シングルセル解析でなにがわかるか

    樫田啓、浅沼浩之( Role: Joint author)

    化学同人  2020.7 

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    Language:Japanese Book type:Scholarly book

  2. 生体材料化学

    浅沼浩之、樫田啓、神谷由紀子( Role: Joint author)

    コロナ社  2015.12 

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    Language:Japanese

  3. 化学便覧応用化学編第7版「DNA工学」

    樫田啓( Role: Sole author)

    丸善出版  2014 

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    Language:Japanese

  4. DNA Conjugates and Sensors

    H. Kashida, H. Asanuma( Role: Joint author)

    RSC Publishing  2012 

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    Language:English

  5. バイオチップ実用化ハンドブック・人工核酸を利用したプローブDNAの調製と遺伝子多型検出

    樫田啓、浅沼浩之( Role: Joint author)

    エヌ・ティー・エス  2010 

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    Language:Japanese

Presentations 104

  1. 人工核酸を基盤とする超分子材料の開発 Invited

    樫田啓

    第72回高分子年次大会  2023.5.26 

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    Event date: 2023.5

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Venue:Gメッセ群馬   Country:Japan  

  2. 人工核酸を利用した機能性材料の開発 Invited

    樫田啓

    第4回発動分子科学サロン 

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    Event date: 2023.1

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Venue:東京工業大学   Country:Japan  

  3. 核酸の多様化を⽬指した⼈⼯核酸の開発 Invited

    樫田啓

    第9回バイオ関連化学シンポジウム若手フォーラム 

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    Event date: 2022.9

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Venue:名古屋大学   Country:Japan  

  4. Development of fluorescent probes detecting RNA Invited International conference

    H. Kashida

    Pacifichem2021  2021.12.21 

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    Event date: 2021.12

    Language:English   Presentation type:Oral presentation (invited, special)  

    Venue:Online   Country:United States  

  5. Precise control of dye interaction by using D-threoninol scaffold Invited International conference

    H. Kashida

    11th International Conference on Porphyrins & Phthalocyanines (ICPP-11)  2021.7.3 

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    Event date: 2021.6 - 2021.7

    Language:English   Presentation type:Oral presentation (invited, special)  

    Venue:Online   Country:United States  

  6. 細胞イメージングに向けた人工核酸の開発 Invited

    樫田啓

    第8回卓越・先端・次世代研究シンポジウム  2021.6.23  名古屋大学高等研究院

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    Event date: 2021.6

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Venue:オンライン   Country:Japan  

  7. 核酸の化学修飾とその応用 Invited

    樫田啓

    第69回高分子討論会 

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    Event date: 2020.9

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Venue:オンライン   Country:Japan  

  8. 単純な構造を持つ核酸の開発及びその応⽤ Invited

    樫田啓

    第5回生体分子科学シンポジウム 

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    Event date: 2019.7

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Venue:京都大学   Country:Japan  

  9. 単純な核酸をつくる・つかう Invited

    樫田啓

    第18回FIT-MEセミナー 

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    Event date: 2019.1

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Venue:福岡工業大学   Country:Japan  

  10. 人工核酸を利用した新規機能性材料・超分子の開発 Invited

    樫田啓

    生体機能関連化学部会若手の会 第30回サマースクール 

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    Event date: 2018.7

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Venue:宮崎   Country:Japan  

  11. 新規人工核酸SNAを用いた生細胞内RNAイメージング Invited

    樫田啓

    日本化学会第98春季年会 

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    Event date: 2018.3

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Venue:日本大学   Country:Japan  

  12. Orientation Dependent FRET as a Tool to Analyze DNA Structures Invited International conference

    H. Kashida

    INTERNATIONAL CONGRESS ON PURE & APPLIED CHEMISTRY 2018 (ICPAC2018) 

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    Event date: 2018.3

    Language:English   Presentation type:Oral presentation (invited, special)  

    Venue:Siem Reap, Cambodia   Country:Cambodia  

  13. DNAはどこまで化学的に入れ換え可能か? Invited

    樫田啓

    東京大学化学生命工学科2017年度談話会 

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    Event date: 2017.12

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Venue:東京大学   Country:Japan  

  14. Analysis of energy transfer between dyes by using DNA scaffold Invited

    Hiromu Kashida

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    Event date: 2017.9

    Language:English   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  15. Chemical synthesis of artificial DNA for functional materials Invited

    Hiromu Kashida

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    Event date: 2017.8

    Language:English   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  16. Molecular design of pseudo base pairs for functionalization of DNA Invited International conference

    H. Kashida

    Annual Spring Meeting of Polymer Society of Korea 

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    Event date: 2017.4

    Language:English   Presentation type:Oral presentation (invited, special)  

    Venue:Daejon   Country:Korea, Republic of  

  17. Development of functional DNA materials by using acyclic scaffolds Invited International conference

    H. Kashida

    CBC SEMINAR 

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    Event date: 2016.12

    Language:English   Presentation type:Oral presentation (invited, special)  

    Venue:Nanyang Technological University   Country:Singapore  

  18. Synthesis of Serinol Nucleic Acid (SNA) Exhibiting Unique Functions Invited

    H. Kashida

    10th Anniversary International Symposium on Nanomedicine (ISNM2016) 

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    Event date: 2016.11

    Language:English   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  19. 非環状骨格を持つ機能性疑似塩基対の開発 Invited

    樫田啓

    第47回中部化学関係学協会支部連合秋季大会 

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    Event date: 2016.11

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Venue:豊橋技術科学大学   Country:Japan  

  20. 新規人工核酸SNAを用いた生細胞内RNAイメージング

    樫田 啓

    日本化学会第96春季年会 

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    Event date: 2016.3

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Venue:同志社大学   Country:Japan  

  21. Development of a robust FRET system for structural analyses of DNA duplexes International conference

    H. Kashida, A. Kurihara, H. Asanuma

    BIT's 6th World Gene Convention-2015 

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    Event date: 2015.11

    Language:English   Presentation type:Oral presentation (invited, special)  

    Country:China  

  22. Design of functional DNA by using pseudo base-pair

    Hiromu Kashida

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    Event date: 2015.9

    Language:English   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  23. Orientation-dependent FRET system for structural analyses of nucleic acids

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    Event date: 2015.7

    Language:English   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  24. Highly-sensitive RNA detection by using In Stem Molecular Beacon International conference

    H. Kashida

    China/Japan Young Chemists Forum 

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    Event date: 2014.8

    Language:English   Presentation type:Oral presentation (invited, special)  

    Country:China  

  25. 機能性核酸材料の創製を目指した人工塩基対の開発

    樫田啓

    第63回高分子学会年次大会 

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    Event date: 2014.5

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Venue:名古屋国際会議場   Country:Japan  

  26. 非天然分子による疑似塩基対を利用した機能性核酸の創製

    樫田啓

    日本化学会第94春季年会 

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    Event date: 2014.3

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Venue:名古屋大学   Country:Japan  

  27. 人工塩基対の開発による機能性核酸の創製

    樫田啓

    九州地区高分子若手研究会・夏の講演会 

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    Event date: 2013.7

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  28. Preparation of novel artificial base pairs for functionalization of DNA

    H. Kashida

    The First International Symposium on Biofunctional Chemistry (ISBC2012) 

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    Event date: 2012.11

    Language:English   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  29. Ru錯体を利用した部位特異的DNA光架橋法の開発

    樫田啓, 東秀憲, 五月女光, 宮坂博, 浅沼浩之

    日本化学会第104春季年会  2024.3.18  日本化学会

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    Event date: 2024.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:日本大学理工学部船橋キャンパス   Country:Japan  

  30. セリノール核酸によるナノ構造体形成を利用したキラ ル増幅

    樫田啓, 西川慧史, 浅沼浩之

    第72回高分子討論会  高分子学会

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    Event date: 2023.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:香川大学幸町キャンパス   Country:Japan  

  31. 二重鎖形成を利用した人工核酸の配列解析

    樫田啓, 香川恵未莉, 清水優香, 愛場雄一郎,荘司長三, 浅沼浩之

    第17回バイオ関連化学シンポジウム  日本化学会

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    Event date: 2023.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:東京理科大学野田キャンパス   Country:Japan  

  32. ペリレンーCy3間FRETを利用したDNA構造解析

    樫田啓, 河合隼人, 浅沼浩之

    2023年光化学討論会  光化学協会

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    Event date: 2023.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:広島国際会議場   Country:Japan  

  33. 人工核酸ナノ構造体を利用したキロプティカルシグナル発現

    樫田啓, 西川慧史, 浅沼浩之

    第33回バイオ・高分子シンポジウム  高分子学会バイオ・高分子研究会

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    Event date: 2023.7

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:東京工業大学大岡山キャンパス   Country:Japan  

  34. 二重鎖形成を利用した人工核酸配列解析法の開発

    樫田啓, 香川恵未莉, 清水優香, 戸谷恵介,愛場雄一郎,荘司長三, 浅沼浩之

    日本化学会第103春季年会  日本化学会

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    Event date: 2023.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:東京理科大学野田キャンパス   Country:Japan  

  35. 人工核酸SNAナノ構造体を利用したキラル増幅

    樫田 啓, 西川慧史, 角田貴洋, 生越友樹, 浅沼浩之

    第14回バイオ関連化学シンポジウム 

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    Event date: 2020.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:オンライン開催   Country:Japan  

  36. 人工核酸を利用した六重鎖の開発

    樫田 啓, 服部悠平, 浅沼浩之

    第30回バイオ・高分子シンポジウム 

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    Event date: 2020.7

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:オンライン開催   Country:Japan  

  37. 人工核酸を利用したキラル増幅系の開発

    樫田 啓, 西川慧史, 林 一陽, 角田貴洋, 生越友樹, 浅沼浩之

    第69回高分子学会年次大会 

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    Event date: 2020.5

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:福岡国際会議場[開催中止]   Country:Japan  

  38. Hexaplex formation in aqueous solution

    Hiromu Kashida, Yuhei Hattori, Hiroyuki Asanuma

    The 12th SPSJ International Polymer Conference (IPC2018) 

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    Event date: 2018.12

    Language:English   Presentation type:Oral presentation (general)  

    Country:Japan  

  39. Hexaplex formation by artificial nucleic acid tethering bifacial nucleobases

    Hiromu Kashida, Yuhei Hattori, Kentaro Ishii, Susumu Uchiyama, Hiroyuki Asanuma

    ISNAC2018 

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    Event date: 2018.11

    Language:English   Presentation type:Oral presentation (general)  

    Country:Japan  

  40. RNA detection by a stem-less probe using pairing between fluorophores and quenchers International conference

    Hiromu Kashida, Kazuhiro Morimoto Hiroyuki Asanuma

    23rd International Roundtable on Nucleosides, Nucleotide and Nucleic Acids (IRT2018) 

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    Event date: 2018.8

    Language:English   Presentation type:Poster presentation  

    Venue:San Diego   Country:Japan  

  41. Observation of orientation dependence of FRET by using DNA scaffold International conference

    Hiromu Kashida, Ayako Kurihara, Tomohiro Kato, Hiroyuki Asanuma

    International Symposium on Photochemistry (27th PhotoIUPAC) 

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    Event date: 2018.7

    Language:English   Presentation type:Oral presentation (general)  

    Venue:Dublin   Country:Japan  

  42. 人工核酸を利用した六重鎖の調製

    樫田 啓, 服部悠平, 中村健人, 浅沼 浩之

    日本化学会第99春季年会 

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    Event date: 2018.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:甲南大学   Country:Japan  

  43. DNAを光反応場として利用した同種色素間エネ ルギー移動の解析

    樫田啓, 河合隼人, 荒木保幸, 和田健彦, 浅沼浩之

    第11回バイオ関連化学シンポジウム 

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    Event date: 2017.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:東京大学   Country:Japan  

  44. DNA骨格を利用した同種色素間エネルギー移動の詳細な解析

    樫田 啓, 河合隼人, 荒木保幸, 和田健彦, 浅沼 浩之

    2017年光化学討論会 

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    Event date: 2017.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:東北大学   Country:Japan  

  45. 安定な色素ペアを利用した高感度RNA検出

    樫田 啓, 森本一弘, 浅沼 浩之

    日本化学会第97春季年会 

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    Event date: 2017.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:慶応義塾大学   Country:Japan  

  46. Accumulation of dyes into DNA for efficient light-harvesting

    Hiromu Kashida, Ryoko Maruyama, Hayato Kawai, Yasuyuki Araki, Takehiko Wada, Hiroyuki Asanuma

    The 11th SPSJ International Polymer Conference (IPC 2016) 

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    Event date: 2016.12

    Language:English   Presentation type:Oral presentation (general)  

    Country:Japan  

  47. Development of Artificial Nucleic Acids for Sensitive Detection of RNA in Cell International conference

    H. Kashida

    NUS-JST(PRESTO) Workshop on BioImaging and Single Cell Analysis 

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    Event date: 2016.12

    Language:English   Presentation type:Oral presentation (general)  

    Country:Singapore  

  48. Structural analyses of nicked and gapped DNA duplexes by using orientation dependence of FRET

    Hiromu Kashida, Ayako Kurihara, Hiroyuki Asanuma

    The 43rd International Symposium on Nucleic Acid Chemistry (ISNAC2016) 

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    Event date: 2016.9

    Language:English   Presentation type:Oral presentation (general)  

    Country:Japan  

  49. Development of a linear probe tethering Cy3 for RNA detection in cell

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    Event date: 2016.9

    Language:English   Presentation type:Oral presentation (general)  

    Country:Japan  

  50. ステム構造不要なCy3導入リニアプローブによるRNA検出

    樫田 啓, 森本一弘, 浅沼 浩之

    第10回バイオ関連化学シンポジウム 

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    Event date: 2016.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:石川県立音楽堂   Country:Japan  

  51. DNA骨格を利用したエネルギーマイグレーションの検討

    樫田 啓, 丸山 諒子, 河合隼人, 荒木保幸, 和田健彦, 浅沼 浩之

    2016年光化学討論会 

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    Event date: 2016.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:東京大学   Country:Japan  

  52. FRETの距離・配向依存性を利用した核酸構造解析法の開発

    樫田 啓, 栗原 綾子, 浅沼 浩之

    第26回バイオ・高分子シンポジウム 

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    Event date: 2016.7

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:東京工業大学   Country:Japan  

  53. 高効率光捕集系の構築に向けた蛍光色素集積化DNAの調製

    樫田 啓, 丸山 諒子, 栗原 綾子, 浅沼 浩之

    第65回高分子学会年次大会 

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    Event date: 2016.5

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:神戸   Country:Japan  

  54. DEVELOPMENT OF A ROBUST FRET SYSTEM BY USING D-THREONINOL SCAFOLD International conference

    H. Kashida

    26th IUPAC International Symposium on Photochemistry 

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    Event date: 2016.4

    Language:English   Presentation type:Oral presentation (general)  

    Country:Japan  

  55. FRETの配向依存性を利用したDNA二重鎖中のGap構造解析

    樫田 啓, 栗原綾子, 浅沼浩之

    日本化学会第96春季年会 

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    Event date: 2016.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:同志社大学   Country:Japan  

  56. Development of orientation-dependent FRET system for structural analyses of nucleic acids International conference

    H. Kashida, A. Kurihara, H. Asanuma

    Pacifichem2015 

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    Event date: 2015.12

    Language:English   Presentation type:Poster presentation  

    Country:United States  

  57. Structural analysis of gapped DNA duplex by using orientation dependent FRET system

    Hiromu Kashida, Ayako Kurihara, Hiroyuki Asanuma

    The 42nd International Symposium on Nucleic Acid Chemistry 

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    Event date: 2015.9

    Language:English   Presentation type:Poster presentation  

    Country:Japan  

  58. 電荷移動相互作用を利用したヘテロ選択的人工二重鎖形成

    樫田 啓、榊原拓海、土居哲也、荒木保幸、和田健彦、浅沼浩之

    第64回高分子討論会 

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    Event date: 2015.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:東北大学   Country:Japan  

  59. 配向依存型FRETを利用したDNAのGap構造解析

    樫田 啓、栗原綾子、浅沼浩之

    第9回バイオ関連化学シンポジウム 

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    Event date: 2015.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:熊本大学   Country:Japan  

  60. DNA structural analysis by using orientation-dependent FRET system

    H. Kashida, A. Kurihara, H. Asanuma

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    Event date: 2015.5

    Language:English   Presentation type:Oral presentation (general)  

    Country:Japan  

  61. Structural analysis of dye-modified DNA duplex by using FRET

    H. Kashida, A. Kurihara, H. Asanuma

    The 41st International Symposium on Nucleic Acid Chemistry (ISNAC2014) 

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    Event date: 2014.11

    Language:English   Presentation type:Oral presentation (general)  

    Country:Japan  

  62. DNA二重鎖を利用した配向依存型FRETシステムの開発

    樫田啓,加藤智博,栗原綾子,浅沼浩之

    第8回バイオ関連化学シンポジウム 

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    Event date: 2014.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:岡山大学   Country:Japan  

  63. Synthesis of Serinol Nucleic Acid (SNA) with unique properties International conference

    H. Kashida, K. Murayama, H. Asanuma

    The 40th International Symposium on Nucleic Acid Chemistry (ISNAC2013) 

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    Event date: 2013.11

    Language:English   Presentation type:Oral presentation (general)  

    Country:Japan  

  64. Cy3 複数導入インステムモレキュラービーコンによる高感度核酸検出

    樫田啓,大澤卓矢,神谷由紀子,浅沼浩之

    第23回バイオ・高分子シンポジウム 

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    Event date: 2013.7 - 2013.8

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  65. Development of a robust model system of FRET within DNA duplex International conference

    H. Kashida, T. Kato, H. Asanuma

    26th International Conference on Photochemistry (ICP 2013) 

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    Event date: 2013.7

    Language:English   Presentation type:Oral presentation (general)  

    Country:Belgium  

  66. A Study on FRET System Between Dyes Introduced within DNA Duplex

    H. Kashida, T. Kato, H. Kishida, H. Yada, H. Okamoto, H. Asanuma

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    Event date: 2013.5

    Language:English   Presentation type:Oral presentation (general)  

    Country:Japan  

  67. DNA二重鎖内部へ付加的に導入された色素間のFRET に関する研究

    加藤智博,樫田啓,岸田英夫,矢田祐之,岡本博,浅沼浩之

    日本化学会第93春季年会 

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    Event date: 2013.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  68. DNA二重鎖を利用した色素会合体のらせん制御

    樫田啓、土居哲也、藤井大雅、浅沼浩之

    第61回高分子討論会 

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    Event date: 2012.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  69. 励起子相互作用を利用した高感度モレキュラービーコンの開発

    樫田啓、藤井大雅、原雄一、吉田安子、浅沼浩之

    2012年光化学討論会 

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    Event date: 2012.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:東京工業大学   Country:Japan  

  70. リン酸アニオンを認識する蛍光性核酸プローブの開発

    樫田啓、土居哲也、藤井大雅、浅沼浩之

    第6回バイオ関連化学シンポジウム 

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    Event date: 2012.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:北海道大学   Country:Japan  

  71. Design of novel artificial base pairs for the suppression of electron transfer in DNA duplex International conference

    H. Kashida, K. Sekiguchi, T. Kato, H. Asanuma

    XXIVth IUPAC Symposium on Photochemistry 

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    Event date: 2012.7

    Language:English   Presentation type:Oral presentation (general)  

    Country:Portugal  

  72. エキシプレックス発光を利用した三塩基欠失検出モレキュラービーコンの開発

    樫田啓,関口康司,浅沼浩之

    第22回バイオ・高分子シンポジウム 

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    Event date: 2012.6

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  73. Effect of chirality of threoninol linker on the stability of DNA duplex International conference

    H. Kashida, T. Doi, T. Fujii, H. Asanuma

    The 24th International Symposium on Chirality  

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    Event date: 2012.6

    Language:English   Presentation type:Oral presentation (general)  

    Country:United States  

  74. Design of novel artificial base pairs for the enhancement of fluorescence

    H. Kashida, K. Sekiguchi, T. Kato, H. Asanuma

    The 38th International Symposium on Nucleic Acid Chemistry 

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    Event date: 2011.11

    Language:English   Presentation type:Oral presentation (general)  

    Country:Japan  

  75. カチオン性色素会合を利用した光機能性DNA グルーの開発

    樫田啓,林威光,土居哲也,浅沼浩之

    第5回バイオ関連化学シンポジウム 

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    Event date: 2011.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:つくば   Country:Japan  

  76. スチルバゾールの光二量化反応を利用したDNAの光架橋

    樫田啓、林威光、土居哲也、浅沼浩之

    2011年光化学討論会 

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    Event date: 2011.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  77. 蛍光色素の高輝度化を目指した新規人工塩基対の開発

    樫田啓,関口康司,浅沼浩之

    第21回バイオ・高分子シンポジウム 

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    Event date: 2011.7

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  78. Development of Insulator Base Pairs for the Drastic Enhancement of Quantum International conference

    H. Kashida, K. Sekiguchi, H. Asanuma

    XVth Symposium on Chemistry of Nucleic Acid Components 

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    Event date: 2011.6

    Language:English   Presentation type:Oral presentation (general)  

    Country:Czech Republic  

  79. Strong adhesion between full-matched DNA

    H. Kashida, T. Hayashi, T. Fujii, H. Asanuma

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    Event date: 2011.5

    Language:English   Presentation type:Oral presentation (general)  

    Country:Japan  

  80. DNA 二重鎖の強固な連結を目指したカチオン性人工塩基対の開発

    樫田啓・林威光・藤井大雅・浅沼浩之

    日本化学会第91春季年会 

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    Event date: 2011.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:日本化学会第91春季年会(2011)講演予稿集   Country:Japan  

  81. DNA dot: Accumulation of fluorophores in DNA duplex for the enhancement of emission International conference

    H. Kashida; K. Sekiguchi; H. Asanuma

    PACIFICHEM 2010 

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    Event date: 2010.12

    Language:English   Presentation type:Oral presentation (general)  

    Country:United States  

  82. "Base pairing" of cationic dyes for the stabilization of DNA duplex International conference

    H. Kashida; T. Hayashi; T. Fujii; H. Asanuma

    PACIFICHEM 2010 

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    Event date: 2010.12

    Language:English   Presentation type:Poster presentation  

    Country:United States  

  83. Synthesis of novel artificial nucleic acid by using chiral serinol as a linker

    H. Kashida, K. Murayama, T. Toda, H. Asanuma

    The 37th International Symposium on Nucleic Acids Chemistry  

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    Event date: 2010.11

    Language:English   Presentation type:Poster presentation  

    Country:Japan  

  84. 蛍光色素の高輝度化を目指したインスレーター塩基対の開発

    樫田 啓、関口 康司、浅沼 浩之

    第4回バイオ関連化学シンポジウム 

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    Event date: 2010.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  85. In Stem Molecular Beacon (IS-MB): A Novel Molecular Beacon with Low Background Emission International conference

    H. Kashida, T. Takatsu, T. Fujii, K. Sekiguchi, X. G. Liang, Y. Yoshida, H. Asanuma

    XVIII International Roundtable on Nucleosides, Nucleotides and Nucleic Acids 

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    Event date: 2010.8 - 2010.9

    Language:English   Presentation type:Poster presentation  

    Country:France  

  86. カチオン性色素クラスター形成によるDNA二重鎖の連結

    樫田啓・林威光・藤井大雅・浅沼浩之

    第20回バイオ・高分子シンポジウム 

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    Event date: 2010.7

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  87. カチオン性色素のクラスター形成を利用したDNAグルーの開発

    樫田啓・林威光・藤井大雅・浅沼浩之

    第59回高分子年次大会 

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    Event date: 2010.5

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  88. 蛍光色素の量子収率向上を目指した“インスレーター塩基対”の開発(1)高感度蛍光ラベル化剤開発に向けた”インスレーター塩基対”の設計

    樫田啓・関口康司・浅沼浩之

    日本化学会第90春季年会 

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    Event date: 2010.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  89. Efficient energy transfer from pyrene to perylene assembled inside DNA duplex

    The 6th International symposium on Nucleic Acid Chemistry 

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    Event date: 2009.9

    Language:English   Presentation type:Oral presentation (general)  

    Country:Japan  

  90. ステム内部での色素会合を利用した高感度モレキュラービーコンの開発

    樫田啓・高津智彦・梁興国・丹羽孝介・高瀬智和・吉田安子・浅沼浩之

    第58回高分子討論会 

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    Event date: 2009.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  91. ペリレン-アントラキノン塩基対を利用した高感度In-Stem Molecular Beaconの開発

    樫田啓・高津智彦・関口康司・梁興国・丹羽孝介・高瀬智和・吉田安子・浅沼浩之

    第24回生体機能関連化学シンポジウム 

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    Event date: 2009.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  92. DNA内部での蛍光色素会合を利用した高感度ラベル化法の開発

    樫田啓・関口康司・高津智彦・浅沼浩之

    第19回バイオ・高分子シンポジウム 

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    Event date: 2009.7

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  93. Distinct Stabilization of DNA Duplex by IntroducingCationic Dye Cluster International conference

    International Conference on Materials for Advanced Technologies 2009 

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    Event date: 2009.7

    Language:English   Presentation type:Oral presentation (invited, special)  

  94. カチオン性色素による"塩基対"導入によるDNA二重鎖の安定化

    樫田 啓・伊藤 栄紘・藤井 大雅・浅沼 浩之

    日本化学会第89春季年会 

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    Event date: 2009.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  95. ペリレン導入Threoninol nucleotideを活用した高感度モレキュラービーコンの設計

    高津 智彦・丹羽 孝介・高瀬 智和・吉田 安子・梁 興国・樫田 啓・浅沼 浩之

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    Event date: 2009.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  96. カチオン性色素会合を利用したDNA二重鎖の安定化

    樫田啓・伊藤栄紘・藤井大雅・浅沼浩之

    第3回バイオ関連化学合同シンポジウム 

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    Event date: 2008.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  97. Incorporation of cationic dyes into DNA for distinct stabilization of duplex International conference

    Joint Symposium of 18th International Roundtable on Nucleosides, Nucleotides and Nucleic Acids and 35th International Symposium on Nucleic Acids Chemistry 

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    Event date: 2008.9

    Language:English   Presentation type:Poster presentation  

    Country:Japan  

  98. Preparation of dye aggregates by means of DNA scaffolds International conference

    XXII IUPAC SYMPOSIUM ON PHOTOCHEMISTRY 

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    Event date: 2008.7

    Language:English   Presentation type:Poster presentation  

  99. 蛍光色素導入DNAを用いた塩基欠失多型の高感度検出

    第18回バイオ・高分子シンポジウム 

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    Event date: 2008.7

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  100. 色素会合を利用したDNA二重鎖の安定化

    第57回高分子学会年次大会 

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    Event date: 2008.5

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  101. メロシアニン系色素導入DNAを用いた新規蛍光プローブの開発

    樫田啓・佐野香苗・浅沼浩之

    日本化学会第88春季年会 

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    Event date: 2008.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  102. Construction of novel dye aggregates based on"comb-type" sequence International conference

    SNAC2007 5th International Symposium on Nucleic Acids Chemistry 

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    Event date: 2007.11

    Language:English   Presentation type:Oral presentation (general)  

    Country:Japan  

  103. DNA 二重鎖形成に伴う“くし型”会合を利用したホモ色素クラスターの調製

    第22回生体機能関連化学シンポジウム 

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    Event date: 2007.9

    Language:Japanese   Presentation type:Poster presentation  

    Country:Japan  

  104. DNAの二重鎖形成を利用したくし型色素クラスターの調製

    樫田啓・藤井大雅・浅沼浩之

    第17回バイオ・高分子シンポジウム 

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    Event date: 2007.7

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

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Research Project for Joint Research, Competitive Funding, etc. 8

  1. 新規配列解析法を利用した機能性PNAアプタマーの開発

    2022.10 - 2026.3

    科学技術振興機構  さきがけ 

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    Authorship:Principal investigator  Grant type:Competitive

  2. 人工核酸を利用した細胞内タンパク質検出

    2020.4 - 2022.3

    中谷医工計測技術振興財団 奨励研究 

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    Grant type:Competitive

    Grant amount:\2000000 ( Direct Cost: \2000000 )

  3. 核酸の二重鎖形成を利用した生体分子の網羅的解析

    2019.4 - 2021.3

    旭硝子財団 研究奨励 

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    Grant type:Competitive

    Grant amount:\2000000 ( Direct Cost: \2000000 )

  4. 新規人工核酸SNAを用いた生細胞内RNAイメージング

    2014.10 - 2018.3

    さきがけ 

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    Grant type:Competitive

  5. 塩基欠失多型を高感度に検出する蛍光性核酸プローブの開発

    2012.4 - 2014.3

    内藤記念科学奨励金(研究助成) 

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    Grant type:Competitive

  6. 人工塩基対形成を利用した高感度モレキュラービーコンの開発

    2011.9 - 2012.3

    A-STEP FSステージ 探索タイプ 

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    Grant type:Competitive

  7. リン酸アニオンの認識による二重鎖形成検出プローブの開発

    2009.10 - 2010.3

    立松財団研究助成 

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    Grant type:Competitive

  8. エネルギー移動を利用した遺伝子多型検出の高感度化

    2009.4 - 2010.3

    H21年度シーズ発掘試験 

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    Grant type:Competitive

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KAKENHI (Grants-in-Aid for Scientific Research) 13

  1. Meso-Hierarchy Figuration

    Grant number:23H04873  2023.4 - 2028.3

    Grants-in-Aid for Scientific Research  Grant-in-Aid for Transformative Research Areas (A)

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    Authorship:Coinvestigator(s) 

  2. Novel sequencing method for serinol nucleic acid by using hybridization

    Grant number:23H02081  2023.4 - 2026.3

    Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

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    Authorship:Principal investigator 

    Grant amount:\18850000 ( Direct Cost: \14500000 、 Indirect Cost:\4350000 )

  3. 蛍光バーコードを利用したインタラクトームイメージング法の開発

    Grant number:22K19107  2022.6 - 2024.3

    科学研究費助成事業  挑戦的研究(萌芽)

    樫田 啓

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    Authorship:Principal investigator 

    Grant amount:\6500000 ( Direct Cost: \5000000 、 Indirect Cost:\1500000 )

    我々は核酸の鎖交換反応を利用することで多数の分子を同時にラベル化する蛍光バーコードの開発に成功した。この蛍光バーコードは蛍光色素の種類や蛍光変化回数を増加させることで、ラベルの種類を指数関数的に増大できるという特長がある。そこで、本研究では蛍光バーコードを利用することで生体分子間相互作用を可視化する手法の開発を目指す。
    これまでに人工核酸の鎖交換反応を利用することで、蛍光色が”配列”に従って変化する蛍光バーコードの開発に成功している。本研究では、これを更に発展させることで生体分子間相互作用を可視化することを目指す。具体的には1)蛍光バーコードの拡張、及び2)それを利用した生体分子間相互作用解析について検討を行う予定である。
    本年度は1)の蛍光バーコードの拡張において重要となる人工核酸の鎖交換反応の速度論について重点的に解析を行った。具体的には我々が開発した人工核酸であるトレオニノール核酸(aTNA)及びセリノール核酸(SNA)のtoehold長を変化させ、系統的に鎖交換反応の速度を解析した。また、同様の配列をもつDNAとの比較を行った。その結果、人工核酸の鎖交換反応速度もDNA同様のtoehold長依存性を示すものの、低温においてはDNAと比較して反応が若干遅いことがわかった。これは人工核酸が高い二重鎖安定性を示すことに起因すると考えられる。一方、より高温条件下で同様の測定を行ったところ、人工核酸特にaTNAの鎖交換反応が大幅に高速化することが明らかとなった。このことは、aTNAを利用することで温度駆動型の論理回路を開発できることを示唆している。また、蛍光バーコードの蛍光色変化を高速化するためには室温より高温の温度が望ましいことがわかった。更に、aTNAのキラリティに応じて鎖交換反応の直交性を示すことが明らかとなった。
    これらの成果は現在論文投稿準備中である。
    本研究では2年間の研究期間において、1)蛍光バーコードの拡張、及び2)それを利用した生体分子間相互作用解析について検討を行う予定である。
    本年度は1)蛍光バーコードの拡張において重要となる人工核酸の鎖交換反応の速度論的解析について主に検討した。その結果、人工核酸の鎖交換反応速度が温度に大きく依存することを明らかにした。これは蛍光バーコードを拡張する際の最適温度を探索する上で重要な知見である。
    以上のことから、本研究はおおむね順調に進展していると判断した。
    研究期間最終年度となる次年度は人工核酸の鎖交換回数を増大させることで、蛍光バーコードの更なる拡張を目指す。その際、今年度明らかにした人工核酸の鎖交換反応速度を利用することで、蛍光バーコードの駆動速度を最適化したい。また、蛍光バーコードを抗体に固定化することによる、生体分子間相互作用解析について検討を行う予定である。

  4. Development of Curved Nanowires and Their Application to Nanotechnology

    Grant number:22H00331  2022.4 - 2026.3

    Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (A)

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    Authorship:Coinvestigator(s) 

  5. Detection of biomolecules by using chiral amplification system composed of artificial nucleic acids

    Grant number:20H02858  2020.4 - 2023.3

    Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

    Kashida Hiromu

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    Authorship:Principal investigator 

    Grant amount:\17940000 ( Direct Cost: \13800000 、 Indirect Cost:\4140000 )

    In this study, we aimed to develop a chiral amplification system utilizing serinol nucleic acid (SNA). First, we prepared a one-dimensional nano structure by hybridizing achiral SNA strands. When chiral nucleic acid was added, CD intensity drastically increased. Thus, chiral amplification system triggered by duplex formation was successfully developed for the first time. Then we modified SNA nano structures with pyrene. The structure expressed circularly polarized luminescence (CPL) upon the addition of chiral nucleic acids. We also succeeded to develop a chiral amplification system triggered by DNA.

  6. Development of fluorobarcode that can detect multiple biomolecules simultaneously

    Grant number:19K22250  2019.6 - 2021.3

    Grants-in-Aid for Scientific Research  Grant-in-Aid for Challenging Research (Exploratory)

    Kashida Hiromu

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    Authorship:Principal investigator 

    Grant amount:\6500000 ( Direct Cost: \5000000 、 Indirect Cost:\1500000 )

    Fluorescent imaging is one of the most essential tools in biology. However, the number of detectable molecules was severely restricted due to spectral overlaps of fluorophores. In this study, we aimed to prepare fluorescent barcode, in which emission color changes depending on the color sequence. We found that emission color of fluorescent barcode can change in pre-determined order. We also succeeded in multiplexed imaging of polystyrene beads and proteins in cell.

  7. Investigation of dye interaction and preparation of functional pi-assembly figuration by DNA scaffold

    Grant number:17H05150  2017.4 - 2019.3

    Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

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    Authorship:Principal investigator 

    Grant amount:\6240000 ( Direct Cost: \4800000 、 Indirect Cost:\1440000 )

  8. Novel method for structural analyses of nucleic acids by using orientation dependent FRET system

    Grant number:16H05925  2016.4 - 2020.3

    Kashida Hiromu

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    Authorship:Principal investigator 

    Grant amount:\25350000 ( Direct Cost: \19500000 、 Indirect Cost:\5850000 )

    In this study, we aimed to develop a versatile method to analyze double helical structures of nucleic acids by using orientation dependence of Forster resonance energy transfer (FRET). First, we developed another FRET pair for analyses of larger complexes of nucleic acids. We also found that energy transfer between identical chromophores can also be used for structural analyses. Damaged DNA like gapped DNA, A-tract and RNA structures were successfully analyzed by using the developed method. Furthermore, we succeeded in analyses of the interaction between DNA and small molecules by using orientation dependence of FRET.

  9. Development of artificial nucleic acids forming multi-stranded structures

    Grant number:16K14031  2016.4 - 2018.3

    Kashida Hiromu

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    Authorship:Principal investigator 

    Grant amount:\3770000 ( Direct Cost: \2900000 、 Indirect Cost:\870000 )

    Natural nucleic acids can form a triplex and a quadruplex by forming hydrogen bonds at both sides of purine bases. In this study, we synthesized novel artificial nucleic acids bearing novel nucleobases, which have hydrogen bonding sites at both sides. And we found that a hexaplex was successfully formed by these nucleic acids. The hexaplex was stabilized in the presence of divalent cations and low pH, indicating the potential of the hexaplex as pH and metal ion sensors. Molecular modelling calculation indicated that the hexaplex has a very unique structure with a pore at its center. It could be applied as novel supramolecular motifs and ion channels.

  10. 細胞内イメージングに向けた超高感度核酸プローブの開発

    2014.4 - 2016.3

    科学研究費補助金  新学術領域研究(研究領域提案型・公募研究)

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    Authorship:Principal investigator 

  11. 細胞内イメージングに向けた超高感度核酸プローブの開発

    2012.4 - 2014.3

    科学研究費補助金  新学術領域研究(研究領域提案型・公募研究)

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    Authorship:Principal investigator 

  12. “DNAドット”を活用した高感度ラベル化法の開発

    2010.4 - 2012.3

    科学研究費補助金  若手研究(B)

    樫田啓

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    Authorship:Principal investigator 

  13. 核酸とのコンジュゲーションによる色素会合体調製法の確立

    2007.4 - 2009.3

    科学研究費補助金  若手(スタートアップ)

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    Authorship:Principal investigator 

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Industrial property rights 1

  1. インスレーター及びその利用

    樫田啓、浅沼浩之、関口康司

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    Application no:PCT/JP2011/054555  Date applied:2011.2

    Country of applicant:Domestic  

 

Teaching Experience (On-campus) 45

  1. Supramolecular Biochemistry

    2023

  2. Chemistry and Biotechnology Laboratory 2

    2023

  3. Biomolecular Chemistry 1 with Exercises

    2023

  4. Safety in Laboratory

    2023

  5. Fundamentals of Biomolecular Chemistry

    2022

  6. 化学実験

    2022

  7. Technical Visits in Industrial Plants

    2021

  8. Supramolecular Biochemistry

    2021

  9. Understanding the Material World

    2021

  10. 分子生命化学基礎論

    2020

  11. 化学生命工学実験2

    2020

  12. 生化学2及び演習

    2020

  13. 生命超分子化学

    2019

  14. Core Biochemistry

    2019

  15. 分子生命化学基礎論

    2018

  16. 生物機能工学演習1

    2018

  17. Understanding the Material World

    2018

  18. Understanding the Material World

    2017

  19. ナノバイオ計測化学

    2017

  20. Core Biochemistry

    2017

  21. 生命超分子化学

    2017

  22. 生物機能工学演習1

    2017

  23. 生物機能工学演習1

    2016

  24. Understanding the Material World

    2016

  25. 生体材料工学特論

    2015

  26. 生物機能工学演習1

    2015

  27. 分析化学実験第1

    2015

  28. Understanding the Material World

    2015

  29. 分析化学実験第1

    2014

  30. 生物機能工学演習1

    2014

  31. 生物機能工学実験

    2014

  32. 生物機能工学演習1

    2013

  33. 分析化学実験第1

    2013

  34. 生体材料工学特論

    2013

  35. Understanding the Material World

    2013

  36. 生物機能工学実験

    2013

  37. 生物機能工学実験

    2012

  38. 生物機能工学演習1

    2012

  39. Understanding the Material World

    2012

  40. 分析化学実験第1

    2012

  41. 分析化学実験第1

    2011

  42. 生物機能工学演習1

    2011

  43. 生体材料工学特論

    2011

  44. Understanding the Material World

    2011

  45. 生物機能工学実験

    2011

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Teaching Experience (Off-campus) 5

  1. 生体物質合成化学

    2015.4 - 2016.3 Aichi Institute of Technology)

  2. 生体物質合成化学

    2014.4 - 2015.3 Aichi Institute of Technology)

  3. 生体物質合成化学

    2013.4 - 2014.3 Aichi Institute of Technology)

  4. 生体物質合成化学

    2012.4 - 2013.3 Aichi Institute of Technology)

  5. 生体物質合成化学

    2011.4 - 2012.3 Aichi Institute of Technology)

 

Social Contribution 1

  1. 豊田西高校模擬授業

    Role(s):Lecturer

    豊田西高校  2017.11

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    Audience: High school students

    Type:Visiting lecture