Updated on 2024/10/18

写真a

 
ITO Akira
 
Organization
Graduate School of Engineering Chemical Systems Engineering 1 Professor
Graduate School
Graduate School of Engineering
Undergraduate School
School of Engineering Materials Science and Engineering
Title
Professor
Contact information
メールアドレス
Homepage
https://www.material.nagoya-u.ac.jp/ItoLab/index-e.html
External link

Degree 1

  1. Dr. of Engineering （ Nagoya University ） 

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Research Interests 20

  1. 遺伝子治療

  2. 合成生物学

  3. 細胞凍結保存

  4. 骨格筋

  5. ティッシュエンジニアリング

  6. 磁性ナノ粒子

  7. 再生医療

  8. バイオプロセス

  9. ナノメディシン

  10. がん治療

  11. iPS細胞

  12. 膵島

  13. 組織培養

  14. 細胞培養

  15. 細胞凍結保存

  16. 骨格筋

  17. 遺伝子治療

  18. 磁性ナノ粒子

  19. 合成生物学

  20. ティッシュエンジニアリング

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Research Areas 2

  1. Others / Others  / Bioengineering

  2. Manufacturing Technology (Mechanical Engineering, Electrical and Electronic Engineering, Chemical Engineering) / Biofunction and bioprocess engineering

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Current Research Project and SDGs 2

  1. Tissue engineering using magnetite nanoparticles

  2. Hyperthermia Using Magnetic Nanoparticles

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Research History 7

  1. Nagoya University   Graduate School of Engineering Chemical Systems Engineering 1   Professor

    2019.5

  2. Nagoya University   School of Engineering   Professor

    2019.5

  3. Kyushu University   Faculty of Engineering   Associate professor

    2006.3 - 2019.4

  4. Nagoya University   Graduate School of Engineering Department of Applied Chemistry, Chemical Engineering and Biotechnology   Assistant

    2006.3

  5. Nagoya University   School of Engineering   Assistant Professor

    2002.2 - 2006.2

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    Country：Japan

  6. Research Fellow of the Japan Society for the Promotion of Science PD

    2002.1 - 2002.2

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    Country：Japan

  7. Research Fellow of the Japan Society for the Promotion of Science DC1

    2000.4 - 2001.12

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    Country：Japan

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Education 2

  1. Nagoya University   Graduate School, Division of Engineering   Department of Biotechnology

    2000.4 - 2001.12

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    Country： Japan

  2. Nagoya University   Faculty of Engineering   Department of Biotechnology

    - 1998

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    Country： Japan

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Professional Memberships 4

  1. 日本癌学会

  2. 日本生物工学会   理事

  3. 日本ハイパーサーミア学会

  4. 化学工学会

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Committee Memberships 2

  1. 日本学術会議   連携会員  

    2013.9   

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    Committee type：Government

  2. 日本学術会議   連携会員  

    2013.9   

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    Committee type：Government

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Awards 18

  1. 読売テクノフォーラム ゴールドメダル賞

    2013   読売新聞グループ本社   磁性微粒子を用いた医療技術の開発

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    Award type：Award from publisher, newspaper, foundation, etc.  Country：Japan

  2. 科学技術分野の文部科学大臣表彰「若手科学者賞」

    2014   文部科学省   磁性ナノ粒子を用いた医療技術に関する研究

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    Country：Japan

  3. 学術賞

    2024.3   公益財団法人永井科学技術財団   機能性磁性ナノ粒子を用いたがん治療法の開発

    井藤　彰

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    Award type：Award from publisher, newspaper, foundation, etc. 

  4. 生物工学奨励賞「照井賞」

    2018   日本生物工学会   機能性磁性ナノ粒子の開発と医療技術への応用に関する生物工学的研究

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    Award type：Award from Japanese society, conference, symposium, etc.  Country：Japan

  5. 化学工学会研究奨励賞（内藤雅喜記念賞）

    2008   化学工学会   機能性磁性ナノ粒子を用いたティッシュエンジニアリング技術の開発

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    Award type：Award from Japanese society, conference, symposium, etc.  Country：Japan

  6. 第４０回東海化学工業会賞

    2005   東海化学工業会  

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    Country：Japan

  7. 平成１６年度日本ハイパーサーミア学会奨励賞

    2004   日本ハイパーサーミア学会  

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    Country：Japan

  8. 第32回生物工学論文賞

    2024.9   日本生物工学会  

    金子 真大・佐藤 愛梨・綾野 賢・藤田 明士・小林 悟朗・井藤 彰

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    Award type：Award from Japanese society, conference, symposium, etc. 

  9. Best Poster Award

    2018   International Conference on the Scientific and Clinical Applications of Magnetic Carriers   Magnetic Force-Based Tissue Engineering of Skeletal Muscle

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    Award type：International academic award (Japan or overseas)  Country：Denmark

  10. 生物工学奨励賞「照井賞」

    2018   日本生物工学会   機能性磁性ナノ粒子の開発と医療技術への応用に関する生物工学的研究

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    Award type：Award from international society, conference, symposium, etc.  Country：Japan

  11. 日本生物工学会論文賞

    2016   日本生物工学会  

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    Award type：Honored in official journal of a scientific society, scientific journal  Country：Japan

  12. Top Reviewer in 2011

    2012   Elsevier  

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    Award type：Honored in official journal of a scientific society, scientific journal  Country：Netherlands

  13. 日本生物工学会論文賞

    2008   日本生物工学会  

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    Award type：Honored in official journal of a scientific society, scientific journal  Country：Japan

  14. Outstanding Paper Award of 2007

    2008   Incorporation of capillary-like structures into dermal cell sheets constructed by magnetic force-based tissue engineering

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    Award type：Honored in official journal of a scientific society, scientific journal  Country：Japan

  15. 日本ハイパーサーミア学会優秀論文賞

    2006   日本ハイパーサーミア学会  

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    Award type：Honored in official journal of a scientific society, scientific journal  Country：Japan

  16. 化学工学会バイオ部会優秀ポスター賞

    2005   化学工学会バイオ部会  

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    Country：Japan

  17. 平成１６年度日本生物工学会論文賞

    2004   日本生物工学会  

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    Country：Japan

  18. 化学工学会バイオ部会優秀ポスター賞

    2003   化学工学会バイオ部会  

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    Country：Japan

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Papers 207

  1. Medical Application of Functionalized Magnetic Nanoparticles Invited Reviewed

    Akira Ito, Masashige Shinkai, Hiroyuki Honda, Takeshi Kobayashi

    Journal of Bioscience and Bioengineering   Vol. 100 ( 1 ) page： 1-11   2005

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    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

  2. Cancer immunotherapy based on intracellular hyperthermia using magnetite nanoparticles: a novel concept of heat-controlled necrosis with heat shock protein expression Invited Reviewed

    Akira Ito, Hiroyuki Honda, Takeshi Kobayashi

    Cancer Immunology Immunotherapy   Vol. 55 ( 3 ) page： 320-328   2005

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    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

  3. In vitro drug testing based on contractile activity of C2C12 cells in an epigenetic drug model Reviewed

    Kazushi Ikeda, Akira Ito, Ryusuke Imada, Masanori Sato, Yoshinori Kawabe, Masamichi Kamihira

    SCIENTIFIC REPORTS   Vol. 7   page： 44570   2017.3

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：NATURE PUBLISHING GROUP  

    Skeletal muscle tissue engineering holds great promise for pharmacological studies. Herein, we demonstrated an in vitro drug testing system using tissue-engineered skeletal muscle constructs. In response to epigenetic drugs, myotube differentiation of C2C12 myoblast cells was promoted in two-dimensional cell cultures, but the levels of contractile force generation of tissue-engineered skeletal muscle constructs prepared by three-dimensional cell cultures were not correlated with the levels of myotube differentiation in two-dimensional cell cultures. In contrast, sarcomere formation and contractile activity in two-dimensional cell cultures were highly correlated with contractile force generation of tissue-engineered skeletal muscle constructs. Among the epigenetic drugs tested, trichostatin A significantly improved contractile force generation of tissue-engineered skeletal muscle constructs. Follistatin expression was also enhanced by trichostatin A treatment, suggesting the importance of follistatin in sarcomere formation of muscular tissues. These observations indicate that contractility data are indispensable for in vitro drug screening.

    DOI： 10.1038/srep44570

    Web of Science

    PubMed

  4. Genetically engineered angiogenic cell sheets using magnetic force-based gene delivery and tissue fabrication techniques. Reviewed

    Hirokazu Akiyama, Akira Ito, Yoshinori Kawabe, Masamichi Kamihira

    Biomaterials   Vol. 31 ( 6 ) page： 1251-1259   2010

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    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

  5. Tissue engineering using magnetite nanoparticles and magnetic force: Heterotypic layers of co-cultured hepatocytes and endothelial cells. Reviewed

    Akira Ito, Yohei Takizawa, Hiroyuki Honda, Kenichiro Hata, Hideaki Kagami, Minoru Ueda, Takeshi Kobayashi

    Tissue Engineering   Vol. 10 ( 5-6 ) page： 833-840   2004

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    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

  6. Magnetically triggered transgene expression in mammalian cells by localized cellular heating of magnetic nanoparticles. Reviewed

    Akira Ito, Ryoji Teranishi, Kazuki Kamei, Masaki Yamaguchi, Akihiko Ono, Shinya Masumoto, Yuto Sonoda, Masanobu Horie, Yoshinori Kawabe, Masamichi Kamihira

    Journal of bioscience and bioengineering   Vol. 128 ( 3 ) page： 355 - 364   2019.9

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    Language：English   Publishing type：Research paper (scientific journal)  

    To develop a remote control system of transgene expression through localized cellular heating of magnetic nanoparticles, a heat-inducible transgene expression system was introduced into mammalian cells. Cells were labeled with magnetic nanoparticles and exposed to an alternating magnetic field. The magnetically labeled cells expressed the transgene in a monolayer and multilayered cell sheets in which cells were heated around the magnetic nanoparticles without an apparent temperature increase in the culture medium. Magnetic cells were also generated by genetically engineering with a ferritin gene, and transgene expression could be induced by exposure to an alternating magnetic field. This approach may be applicable to the development of novel gene therapies in cell-based medicine.

    DOI： 10.1016/j.jbiosc.2019.03.008

    PubMed

  7. Neural differentiation of mouse induced pluripotent stem cells using cadherin gene-engineered PA6 feeder cells. Reviewed

    Paerwen Paerhati, Akira Ito, Kantaro Yoshioka, Kaori Iwamoto, Sho Fujiwara, Masanobu Horie, Yoshinori Kawabe, Masamichi Kamihira

    Journal of bioscience and bioengineering   Vol. 127 ( 5 ) page： 633 - 640   2019.5

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    Language：English   Publishing type：Research paper (scientific journal)  

    Investigating neural differentiation of pluripotent stem cells, including induced pluripotent stem (iPS) cells, is of importance for studying early neural development and providing a potential source of cells for nerve regeneration. Stromal cell-derived inducing activity (SDIA) using PA6 stromal cells promotes neural differentiation of iPS cells. Thus, we hypothesized that cadherin gene-engineered PA6 feeder cells will enhance the performance of SDIA by facilitating cell-cell interactions. Consequently, we created cadherin gene-engineered PA6 cells. Efficiency of neural differentiation from mouse iPS cells on PA6 feeder cells overexpressing E-cadherin gene (46%) or N-cadherin gene (27%) was significantly higher compared with parental PA6 feeder cells (19%). In addition, efficiency of motor neuron differentiation from mouse iPS cells on cadherin-gene engineered feeder cells (E-cadherin, 7.4%; N-cadherin, 11%) was significantly higher compared with parental PA6 feeder cells (4.1%). Altogether, these results indicate that cadherin gene-engineered feeder cells are a potent tool for promoting neural differentiation of pluripotent stem cells.

    DOI： 10.1016/j.jbiosc.2018.10.009

    PubMed

  8. Fabricating Muscle-Neuron Constructs with Improved Contractile Force Generation. Reviewed

    Arifuzzaman M, Ito A, Ikeda K, Kawabe Y, Kamihira M

    Tissue engineering. Part A   Vol. 25 ( 7-8 ) page： 563-574   2019.4

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1089/ten.TEA.2018.0165

    PubMed

  9. Induction of functional tissue-engineered skeletal muscle constructs by defined electrical stimulation. Reviewed

    Akira Ito, Yasunori Yamamoto, Masanori Sato, Kazushi Ikeda, Masahiro Yamamoto, Hideaki Fujita, Eiji Nagamori, Yoshinori Kawabe, Masamichi Kamihira

    Scientific Reports   Vol. 4   page： 4781   2014

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    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1038/srep04781

  10. Improved recombinant antibody production by CHO cells using a production enhancer DNA element with repeated transgene integration at a predetermined chromosomal site Reviewed

    Yoshinori Kawabe, Takanori Inao, Shodai Komatsu, Guan Huang, Akira Ito, Takeshi Omasa, Masamichi Kamihira

    JOURNAL OF BIOSCIENCE AND BIOENGINEERING   Vol. 123 ( 3 ) page： 390 - 397   2017.3

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：SOC BIOSCIENCE BIOENGINEERING JAPAN  

    Chinese hamster ovary (CHO) cells are one of the most useful host cell lines for the production of biopharmaceutical proteins. Although a series of production processes have been refined to improve protein productivity and cost performance, establishing producer cells is still time-consuming and labor-intensive. Recombinase-mediated site-specific gene integration into a predetermined chromosomal locus may enable predictable protein expression, reducing the laborious process of cell screening. We previously developed an accumulative site-specific gene integration system (AGIS) using Cre recombinase and mutated loxP sites for transgene integration and amplification in the CHO cell genome. Epigenetic modifier elements such as insulators are effective DNA cis-regulatory elements for stabilizing transgene expression. Here, we attempted to enhance transgene expression in recombinant CHO cells generated by AGIS using a production enhancer DNA element (PE) derived from the CHO genome. The PE was introduced into an expression unit for a recombinant scFv-Fc antibody. The effect on scFv-Fc productivity of PE position and orientation within the transgene was evaluated, while keeping the background chromosomal structure constant. For the optimal PE arrangement, scFv-Fc productivity was enhanced 2.6-fold compared with an expression unit without a PE. The enhancing effect of the PE on transgene expression was also observed when two or three PE-flanked expression units were inserted as tandem repeats. These results indicate that AGIS using the PE-flanked expression unit is a promising approach for establishing producer cell lines for biopharmaceutical protein production. (C) 2016, The Society for Biotechnology, Japan. All rights reserved.

    DOI： 10.1016/j.jbiosc.2016.10.011

    Web of Science

    PubMed

  11. Novel therapy for pancreatic fistula using adipose-derived stem cell sheets treated with mannose. Reviewed

    Hirokazu Kaneko, Toshio Kokuryo, Yukihiro Yokoyama, Junpei Yamaguchi, Tokunori Yamamoto, Rei Shibata, Momokazu Gotoh, Toyoaki Murohara, Akira Ito, Masato Nagino

    Surgery   Vol. 161 ( 6 ) page： 1561–1569   2017

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    Language：English   Publishing type：Research paper (scientific journal)  

  12. Targeted transgene insertion into the CHO cell genome using Cre recombinase-incorporating integrase-defective retroviral vectors. Reviewed

    Yoshinori Kawabe, Takuya Shimomura, Shuohao Huang, Suguru Imanishi, Akira Ito, Masamichi Kamihira

    Biotechnology and Bioengineering   Vol. 113 ( 7 ) page： 1600-1610   2016

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    Language：English   Publishing type：Research paper (scientific journal)  

  13. Improved contractile force generation of tissue-engineered skeletal muscle constructs by IGF-I and Bcl-2 gene transfer with electrical pulse stimulation. Reviewed

    Kazushi Ikeda, Akira Ito, Masanori Sato, Yoshinori Kawabe, Masamichi Kamihira

    Regenerative Therapy   Vol. 3   page： 38-44   2016

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    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

  14. A new in vitro co-culture model using magnetic force-based nanotechnology. Reviewed

    Hiroki Takanari, Keiko Miwa, XianMing Fu, Jun-ichi Nakai, Akira Ito, Kousuke Ino, Hiroyuki Honda, Jun Kunishida, Satoru Konishi, Tobias Opthof, Marcel AG van der Heyden, Itsuo Kodama, Jong-Kook Lee

    Journal of Cellular Physiology   Vol. 231 ( 10 ) page： 2249-2256   2016

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    Language：English   Publishing type：Research paper (scientific journal)  

  15. DNA damage-responsive transgene expression mediated by the p53 promoter with transcriptional amplification. Reviewed

    Akihiko Ono, Akira Ito, Taiga Suzuki, Masaki Yamaguchi, Yoshinori Kawabe, Masamichi Kamihira

    Journal of Bioscience and Bioengineering   Vol. 120 ( 4 ) page： 463-466   2015

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    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

  16. Effects of type IV collagen on myogenic characteristics of IGF-I gene-engineered myoblast cells. Reviewed

    Akira Ito, Masahiro Yamamoto, Kazushi Ikeda, Masanori Sato, Yoshinori Kawabe, Masamichi Kamihira

    Journal of Bioscience and Bioengineering.   Vol. 119 ( 5 ) page： 596-603   2015

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    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

  17. Magnetic-labeled feeder system for mouse pluripotent stem cells. Reviewed

    Masanobu Horie, Akira Ito, Takeshi Maki, Yoshinori Kawabe, Masamichi Kamihira

    Journal of Bioscience and Bioengineering.   Vol. 119 ( 5 ) page： 614-616   2015

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    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

  18. Improved transgene integration into the CHO cell genome using the Cre-loxP system. Reviewed

    Takanori Inao, Yoshinori Kawabe, Takuro Yamashiro, Yujiro Kameyama, Xue Wang, Akira Ito, Masamichi Kamihira

    Journal of Bioscience and Bioengineering.   Vol. 120 ( 1 ) page： 99-106   2015

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    Language：English   Publishing type：Research paper (scientific journal)  

  19. Fabrication of 3D tissue-like structure using magnetite nanoparticles and magnetic force.

    Akira Ito, Kosuke Ino, Kazunori Shimizu, Hiroyuki Honda, Masamichi Kamihira

    2006 IEEE International Symposium on Micro-Nano Mechanical and Human Science     2006

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    Authorship：Lead author   Language：English   Publishing type：Research paper (bulletin of university, research institution)  

    DOI： MHS4110394. 5

  20. 磁性ナノ粒子を用いた組織工学手法の開発 Invited

    井藤　彰、本多裕之

    再生医療－日本再生医療学会雑誌   Vol. 5 ( 1 ) page： 84-90   2006

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    Authorship：Lead author   Language：Japanese  

  21. 再生医療への応用 Invited

    井藤　彰、本多裕之

    磁性ビーズのバイオ・環境技術への応用展開     page： 64-74   2006

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    Authorship：Lead author   Language：Japanese  

  22. Magnetic manipulation of a retroviral vector using magnetite cationic liposomes.

    Akira Ito, Tetsuya Takahashi, Yujiro Kameyama, Yoshinori Kawabe, Masamichi Kamihira

    Proceeding of MHS2008     page： 367-371   2008

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    Authorship：Lead author   Language：English   Publishing type：Research paper (bulletin of university, research institution)  

  23. Screening of Cytokines to Enhance Vaccine Effects of Heat Shock Protein 70-Rich Tumor Cell Lysate Reviewed

    Akira Ito, Masatake Fujioka, Kouji Tanaka, Takeshi Kobayashi, Hiroyuki Honda

    Journal of Bioscience and Bioengineering   Vol. 100 ( 1 ) page： 36-42   2005

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    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

  24. Complete Regression of Hereditary Melanoma in a Mouse Model by Repeated Hyperthermia Using Magnetite Cationic Liposomes Reviewed

    Akira Ito, Yoko Nakahara, Masatake Fujioka, Takeshi Kobayashi, Kozue Takeda, Izumi Nakashima, Hiroyuki Honda

    Japanese Journal of Hyperthermic Oncology   Vol. 21 ( 3 ) page： 179-189   2005

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    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

  25. The effect of RGD peptide-conjugated magnetite cationic liposomes on cell growth and cell sheet harvesting Reviewed

    Akira Ito, Kousuke Ino, Takeshi Kobayashi, Hiroyuki Honda

    Biomaterials   Vol. 26 ( 31 ) page： 6185-6193   2005

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    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

  26. Ehancement of hepatocyte function through heterotypic cell-cell interactions using E-cadherin-expressing NIH3T3 cells. Reviewed

    Akira Ito, Takehiko Kiyohara, Yoshinori Kawabe, Hiroyuki Ijima, Masamichi Kamihira

    Proceeding of the 20th JAACT meeting     page： 159-164   2008

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    Authorship：Lead author   Language：English   Publishing type：Research paper (bulletin of university, research institution)  

  27. Heat Immunotherapy Using Magnetic Nanoparticles and Dendritic Cells for T-Lymphoma Reviewed

    Kouji Tanaka, Akira ITO, Takeshi Kobayashi, Tatsuyoshi Kawamura, Shinji Shimada, Kazuhiko Matsumoto, Toshiaki Saida, Hiroyuki Honda

    Journal of Bioscience and Bioengineering   Vol. 100 ( 1 ) page： 112-115   2005

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    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

  28. Magnetic Force-Based Mesenchymal Stem Cell Expansion Using Antibody-Conjugated Magnetoliposomes Reviewed

    Akira Ito, Eri Hibino, Kazunori Shimizu, Takeshi Kobayashi, Yoichi Yamada, Hideharu Hibi, Minoru Ueda, Hiroyuki Honda

    Journal of Biomedical Materials Research   Vol. 75 ( 2 ) page： 320-327   2005

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    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

  29. Intratumoral Injection of Immature Dendritic Cells Enhances Antitumor Effect of Hyperthermia Using Magnetic Nanoparticles Reviewed

    Kouji Tanaka, Akira Ito, Takeshi Kobayashi, Tatsuyoshi Kawamura, Shinji Shimada, Kazuhiko Matsumoto, Toshiaki Saida, Hiroyuki Honda

    International Journal of Cancer   Vol. 116 ( 4 ) page： 624-633   2005

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    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

  30. Anticancer effect of hyperthermia on prostate cancer mediated by magnetite cationic liposomes and immune-response induction in transplanted syngeneic rats. Reviewed

    Noriyasu Kawai, Akira Ito, Yoko Nakahara, Atsuya Hikosaka, Mitsuru Hutakuchi, Tomoyuki Shirai, Hiroyuki Honda, Takeshi Kobayashi, Kenjiro Kohri

    The Prostate   Vol. 64 ( 4 ) page： 373-381   2005

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    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

  31. A Mechanism of Antitumor Immunity Induced by Hyperthermia Invited Reviewed

    Akira Ito, Takeshi Kobayashi, Hiroyuki Honda

    Japanese Journal of Hyperthermic Oncology   Vol. 21 ( 1 ) page： 1-11   2005

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    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

  32. Heat induction of reporter gene expression via the gadd 153 promoter and its possible application to hyperthermia treatment of cancer. Reviewed

    Isabelle Anne Bouhon, Akira Ito, Masashige Shinkai, Hiroyuki Honda, Takeshi Kobayashi

    Cytotechnology   Vol. 33 ( 1 ) page： 131-137   2000

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    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

  33. 磁性ナノ粒子を用いた管状組織の構築

    伊野浩介、井藤 彰、林田真生、小林 猛、松沼 寛、各務秀明、上田 実、本多裕之

    化学工学シンポジウムシリーズ「診断・治療システムと化学工学」   Vol. 79   page： 63-67   2005

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    Authorship：Lead author   Language：Japanese   Publishing type：Research paper (scientific journal)  

  34. 抗体結合型マグネトリポソームを用いた間葉系幹細胞の濃縮培養法

    清水一憲、井藤彰、日比野恵里、小林猛、山田陽一、日比英晴、上田実、本多裕之

    化学工学シンポジウムシリーズ「診断・治療システムと化学工学」   Vol. 79   page： 68-72   2005

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    Authorship：Lead author   Language：Japanese  

  35. 磁性ナノ粒子を利用した再生医療技術"Mag-TE"の開発 Invited

    井藤　彰

    東海化学工業会会報   Vol. 247   page： 14-16   2005

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    Authorship：Lead author   Language：Japanese  

  36. 磁性ビーズの医療への応用

    井藤　彰、本多裕之

    日本応用磁気学会　第141回研究会資料     page： 9-14   2005

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    Authorship：Lead author   Language：Japanese   Publishing type：Research paper (bulletin of university, research institution)  

  37. ナノ磁性微粒子を用いた生体組織構築 Invited

    井藤　彰、本多裕之

    ケミカルエンジニヤリング   Vol. 50 ( 5 ) page： 13-18   2005

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    Authorship：Lead author   Language：Japanese  

  38. メラノーマに対するHeat Immunotherapyの開発 Invited

    井藤　彰、小林　猛、本多裕之

      Vol. 19 ( 3 ) page： 298-305   2005

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    Authorship：Lead author   Language：Japanese  

  39. 免疫誘導型ハイパーサイミア“Heat Immunotherapy”の開発 Invited

    井藤　彰、小林　猛、本多裕之

    癌の臨床   Vol. 50 ( 13 ) page： 1127-1131   2005

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    Authorship：Lead author   Language：Japanese   Publishing type：Research paper (scientific journal)  

  40. Functional magnetic particles for medical application. Invited Reviewed

    Shinkai M, Ito A.

    Adv Biochem Eng Biotechnol   Vol. 91   page： 191-220   2004

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    Authorship：Lead author   Language：English  

  41. Local hyperthermia enhances thrombosis in aneurysms containing platinum coils Reviewed

    Suzuki O, Miyachi S, Okamoto T, Ito A, Shinkai M, Honda H, Kobayashi T, Negoro M, Yoshida J

    Interventional Neuroradiology   Vol. 10 ( 3 ) page： 203-211   2004

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    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

  42. Introduction of TNF-alfa gene expression by heat inducible promoter gadd153. Reviewed

    Akira Ito, Masashige Shinkai, Isabelle Anne Bouhon, Masashige Shinkai, Hiroyuki Honda, Takeshi Kobayashi

    Japanese Journal of Hyperthermic Oncology   Vol. 16 ( 2 ) page： 91-98   2000

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    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

  43. Tissue engineering using magnetite nanoparticles and magnetic force: heterotypic layers of co-cultured hepatocytes and endothelial cells. Reviewed

    Akira Ito, Yohei Takizawa, Hiroyuki Honda, Ken-ichiro Hata, Hideaki Kagami, Minoru Ueda and Takeshi Kobayashi

    Tissue Engineering   Vol. 10 ( 5/6 ) page： 833-840   2004

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    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

  44. A new methodology of mesenchymal stem cell expansion using magnetic nanoparticles Reviewed

    Akira Ito, Eri Hibino, Hiroyuki Honda, Ken-ichiro Hata, Hideaki Kagami, Minoru Ueda and Takeshi Kobayashi

    Biochemical Engineering Journal   Vol. 20   page： 119-125   2004

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    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

  45. Radiation-inducible TNF-alpha gene expression under stress-inducible promoter gadd153 for cancer therapy. Reviewed

    Akira Ito, Masashige Shinkai, Kazumi Hakamada, Hiroyuki Honda, Takeshi Kobayashi

    Journal of Bioscience and Bioengineering   Vol. 92 ( 6 ) page： 598-601   2001

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    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

  46. 磁性微粒子を用いた治療技術開発 Invited

    井藤　彰、本多裕之、小林　猛

    バイオインダストリー   Vol. 21 ( 8 ) page： 48-54   2004

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    Authorship：Lead author   Language：Japanese  

  47. 磁性微粒子を用いたTissue Engineeringの開発 Invited

    井藤　彰、本多裕之

    ケミカルエンジニアリング   Vol. 49 ( 6 ) page： 6-12   2004

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    Authorship：Lead author   Language：Japanese  

  48. 磁性ナノ微粒子を用いたTissue Engineering –Mag-TEの開発- Invited

    井藤　彰、本多裕之、小林　猛

    化学工学会バイオ部会Newsletter   Vol. 10 ( 3 ) page： 1   2004

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    Authorship：Lead author   Language：Japanese  

  49. Construction of Multilayerd Cell Sheets Using Magnetite Nanoparticles and Magnetic Force

    Akira Ito, Hiroyuki Honda, Ken-ichiro Hata, Hideaki Kagami, Minoru Ueda, Takeshi Kobayashi

    Proceding of YABEC'04     page： 142   2004

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  50. Skeletal Muscle Tissue Engineering Using Functional Magnetite Nanoparticles.

    Akira Ito, Hirokazu Akiyama, Yasunori Yamamoto, Yoshinori Kawabe, Masamichi Kamihira

    Proceeding of MHS2009     page： 379-382   2009

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    Authorship：Lead author   Language：English   Publishing type：Research paper (bulletin of university, research institution)  

  51. Heat shock protein 70 gene therapy combined with hyperthermia using magnetic nanoparticles Reviewed

    Akira Ito, Fumiko Matsuoka, Hiroyuki Honda and Takeshi Kobayashi

    Cancer Gene Therapy   Vol. 10 ( 12 ) page： 918-925   2003

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    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

  52. Heat shock protein 70 expression induces an antitumor immunity during intracellular hyperthermia using magnetite nanoparticles Reviewed

    Akira Ito, Masashige Shinkai, Hiroyuki Honda, Kazuhiro Yoshikawa, Shinsuke Saga, Toshihiko Wakabayashi, Jun Yoshida and Takeshi Kobayashi

    Cancer Immunology Immunotherapy   Vol. 52 ( 2 ) page： 80-88   2003

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    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

  53. Transglutaminase-mediated gelatin matrices incorporating cell adhesion factors as a biomaterial for tissue engineering Reviewed

    Akira Ito, Atsunori Mase, Yohei Takizawa, Masashige Shinkai, Hiroyuki Honda, Ken-ichiro Hata, Minoru Ueda and Takeshi Kobayashi

    Journal of Bioscience and Bioengineering   Vol. 95 ( 2 ) page： 196-199   2003

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  54. Tumor regression by combined immunotherapy and hyperthermia using magnetic nanoparticles in an experimental subcutaneous murine melanoma Reviewed

    Akira Ito, Kouji Tanaka, Kazuyoshi Kondo, Masashige Shinkai, Hiroyuki Honda, Kazuhiko Matsumoto, Toshiaki Saida and Takeshi Kobayashi

    Cancer Science   Vol. 94 ( 3 ) page： 308-313   2003

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  55. Proliferation and stratification of keratinocyte on cultured amniotic epithelial cells for tissue engineering Reviewed

    Akira Ito, Yohei Takizawa, Masashige Shinkai, Hiroyuki Honda, Ken-Ichiro Hata, Minoru Ueda, Naohiko Kuno, Atsuo Itakura and Takeshi Kobayashi

    Journal of Bioscience and Bioengineering   Vol. 95 ( 6 ) page： 589-593   2003

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    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

  56. Complete regression of mouse mammary carcinoma with a size greater than 15 mm by frequent repeated hyperthermia using magnetic nanoparticles Reviewed

    Akira Ito, Kouji Tanaka, Hiroyuki Honda, Shigeru Abe, Hideyo Yamaguchi and Takeshi Kobayashi

    Journal of Bioscience and Bioengineering   Vol. 96 ( 4 ) page： 360-363   2003

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    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

  57. Time course of biodistribution and heat generation of magnetite cationic liposomes in mouse model. Reviewed

    Akira Ito, Yoko Nakahara, Kouji Tanaka, Yuko Kuga, Hiroyuki Honda and Takeshi Kobayashi

    Japanese Journal of Hyperthermic Oncology   Vol. 19 ( 3 ) page： 151-159   2003

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    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

  58. Selective hyperthermia using magnetoliposomes to target cervical lymph node metastasis in a rabbit tongue tumor model. Reviewed

    Shigeaki Hamaguchi, Iwai Tohnai, Akira Ito, Kenji Mitsudo, Toshio Shigetomi, Masafumi Ito, Hiroyuki Honda, Takeshi Kobayashi and Minoru Ueda

    Cancer Science   Vol. 94 ( 9 ) page： 834-839   2003

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  59. 磁性微粒子を活用した特異的な診断・治療システム Invited

    井藤　彰、本多裕之、小林　猛

    化学工学   Vol. 67 ( 12 ) page： 692-695   2003

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    Authorship：Lead author   Language：Japanese  

  60. Transglutaminase-mediated gelatin matrices incorporating cell adhesion factors as a biomaterial for tissue engineering. Reviewed

    Akira Ito, Atsuhiko Mase, Yohei Takizawa, Masashige Shinkai, Hiroyuki Honda, Kenichiro Hata, Minoru Ueda, Takeshi Kobayashi

    Journal of Bioscience and Bioengineering   Vol. 95 ( 2 ) page： 196-199   2003

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    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

  61. Bystander-killing effect and cyclic induction of TNF-alfa gene under heat inducible promoter gadd153. Reviewed

    Akira Ito, Masashige Shinkai, Hiroyuki Honda, Takeshi Kobayashi

    Journal of Bioscience and Bioengineering   Vol. 90 ( 4 ) page： 437-441   2000

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    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

  62. Heat-inducible TNF-alpha gene therapy combined with hyperthermia using magnetic nanoparticles as a novel tumor-targeted therapy. Reviewed

    Akira Ito, Masashige Shinkai, Hiroyuki Honda, Takeshi Kobayashi

    Cancer Gene Therapy   Vol. 8 ( 9 ) page： 649-654   2001

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    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

  63. Effective solitary hyperthermia treatment of malignant glioma using stick type CMC-magnetite. In vivo study Reviewed

    Ohno T, Wakabayashi T, Takemura A, Yoshida J, Ito A, Shinkai M, Honda H and Kobayashi T

    Journal of Neuro-Oncology   Vol. 56 ( 3 ) page： 233   2002

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  64. バイオターゲティングのための生体分子デザイン

    井藤　彰、本多裕之、小林　猛

    未来材料   Vol. 2 ( 8 ) page： 5   2002

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    Authorship：Lead author   Language：Japanese  

  65. リポソームを用いた腫瘍への薬剤ターゲティング

    井藤　彰、本多裕之、小林　猛

      Vol. 17 ( 4 ) page： 347   2002

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  66. Radiation-inducible TNF-a gene expression under stress-inducible promoter gadd153 for cancer therapy Reviewed

    Akira Ito, Masashige Shinkai, Kazumi Hakamada, Hiroyuki Honda, Takeshi Kobayashi

    Journal of Bioscience and Bioengineering   Vol. 92 ( 6 ) page： 598   2001

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    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

  67. Heat-inducible TNF-alpha gene therapy combined with hyperthermia using magnetic nanoparticles as a novel tumor-targeted therapy Reviewed

    Akira Ito, Masashige Shinkai, Hiroyuki Honda, Takeshi Kobayashi

    Cancer Gene Therapy   Vol. 8 ( 9 ) page： 649   2001

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    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

  68. Augmentation of MHC class I antigen presentation via heat shock protein expression by hyperthermia Reviewed

    Akira Ito, Masashige Shinkai, Hiroyuki Honda, Toshihiko Wakabayashi, Jun Yoshida, Takeshi Kobayashi

    Cancer Immunology Immunotherapy   Vol. 50 ( 10 ) page： 515   2001

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    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

  69. Bystander-killing effect and cyclic induction of TNF-α gene under heat inducible promoter gadd153 Reviewed

    Akira Ito, Masashige Shinkai, Isabelle Anne Bouhon, Hiroyuki Honda, Takeshi Kobayashi

    Journal of Bioscience and Bioengineering   Vol. 90 ( 4 ) page： 437   2000

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    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

  70. Heat induction of reporter gene expression via the gadd 153 promoter and its possible application to hyperthermia treatment of cancer Reviewed

    Isabelle Anne Bouhon, Akira Ito, Masashige Shinkai, Hiroyuki Honda, Takeshi Kobayashi

    Cytotechnology   Vol. 33 ( 1 ) page： 131   2000

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    Language：English   Publishing type：Research paper (scientific journal)  

  71. Introduction of TNF-α gene expression by heat inducible promoter gadd153 Reviewed

    Akira Ito, Masashige Shinkai, Isabelle Anne Bouhon, Hiroyuki Honda, Takeshi Kobayashi

    Japanese Journal of Hyperthermic Oncology   Vol. 16 ( 2 ) page： 91   2000

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    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

  72. Effective solitary hyperthermia treatment of malignant glioma using stick type CMC-magnetite. In vivo study. Reviewed

    Takanari Ohno, Toshihiko Wakabayashi, Atsuhito Takemura, Jun Yoshida, Akira Ito, Masashige Shinkai, Hiroyuki Honda, Takeshi Kobayashi

    Journal of Neuro-Oncology   Vol. 56 ( 5 ) page： 233-239   2000

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  73. Complete regression of mouse mammary carcinoma with a size greater than 15 mm by frequent repeated hyperthermia using magnetic nanoparticles. Reviewed

    Akira Ito, Koji Tanaka, Hiroyuki Honda, Shigeru Abe, Hideyo Yamaguchi, Takeshi Kobayashi

    Journal of Bioscience and Bioengineering   Vol. 96 ( 4 ) page： 360-363   2003

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    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

  74. ガン治療を目的とした遺伝子発現の誘導

    新海政重、井藤　彰、イザベル　ブホン、本多裕之、小林　猛

    ケミカルエンジニヤリング   Vol. 44   page： 9   1999

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    Language：Japanese  

  75. Bone morphogenic protein signaling inhibitor improves differentiation and function of 3D muscle construct fabricated using C2C12 Reviewed

    Ran Ding, Yuan Xi, Akira Ito, Kazunori Shimizu, Eiji Nagamori, Hideaki Fujita, Takuo Kawamoto, Masanobu Horie

    Journal of Bioscience and Bioengineering   Vol. 137 ( 6 ) page： 480 - 486   2024

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  76. Transplantable cell-encapsulation device using a semipermeable ethylene-vinyl alcohol copolymer membrane in a mouse diabetic model

    Kaneko, M; Moriguchi, H; Futatsubashi, R; Ayano, S; Kobayashi, G; Ito, A

    JOURNAL OF BIOSCIENCE AND BIOENGINEERING   Vol. 136 ( 5 ) page： 415 - 422   2023.11

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    Cell-based therapy is an attractive approach, and encapsulation of therapeutic cells is a promising strategy because it prevents immune responses and allows transplanted cells to be retrieved in case of dysfunction. Bioartificial pancreas, in which insulin-secreting cells are encapsulated in a semipermeable membrane bag, is a new class of medical device for treating type-I diabetes. In this study, we developed a macroencapsulation device in which the pancreatic beta cell line MIN6 was encapsulated in a semipermeable bag made of an ethylene-vinyl alcohol copolymer membrane. In vitro evaluation of ATP and insulin levels revealed that MIN6 cells grown in Matrigel within the device secreted insulin in response to glucose levels. Transplantation of the device lowered blood glucose levels for 30 days in diabetic mice. Histological observation revealed that MIN6 cells formed spheroids in Matrigel, and no host cells were detected within the device. Blood levels of inflammatory cytokines in the transplanted mice were similar to those in non-transplanted mice, and antibody levels in the device were lower than those in the intraperitoneal fluid. These results suggest that the semipermeable ethylene-vinyl alcohol copolymer membrane developed in this study is useful for cell encapsulation in cell-based therapies, including beta-cell macroencapsulation for type-1 diabetes.

    DOI： 10.1016/j.jbiosc.2023.09.001

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  77. A transplantable cell-encapsulation device using a semipermeable ethylene-vinyl alcohol membrane in a mouse diabetic model Reviewed

    Masahiro Kaneko, Hiroaki Moriguchi, Ryo Futatsubashi, Satoru Ayano, Goro Kobayashi, Akira Ito

        2023.9

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  78. 機能性磁性ナノ粒子を用いた再生医療プロセスの開発 Invited

    井藤　彰

    セラミックス   Vol. 58 ( 9 ) page： 584 - 587   2023.9

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    Authorship：Lead author,　Last author,　Corresponding author   Language：Japanese   Publishing type：Research paper (conference, symposium, etc.)  

  79. Expansion of human mesenchymal stem cells on poly(vinyl alcohol) microcarriers Reviewed

    Masahiro Kaneko, Airi Sato, Satoru Ayano, Akio Fujita, Goro Kobayashi, Akira Ito

        2023.8

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    Authorship：Last author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

    DOI： https://doi.org/10.1016/j.jbiosc.2023.08.003

  80. Effective magnetic hyperthermia induced by mitochondria-targeted nanoparticles modified with triphenylphosphonium-containing phospholipid polymers. Reviewed

    Masahiro Kaneko, Hiroto Yamazaki, Takahiro Ono, Masanobu Horie, Akira Ito

    Cancer Science   Vol. 114   page： 3750 - 3758   2023.7

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    Authorship：Last author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1111/cas.15895

  81. Nanowarming of vitrified pancreatic islets as a cryopreservation technology for transplantation

    Wakabayashi, T; Kaneko, M; Nakai, T; Horie, M; Fujimoto, H; Takahashi, M; Tanoue, S; Ito, A

    BIOENGINEERING & TRANSLATIONAL MEDICINE   Vol. 8 ( 4 ) page： e10416   2023.7

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    Language：English   Publisher：Bioengineering and Translational Medicine  

    Biobanking of pancreatic islets for transplantation could solve the shortage of donors, and cryopreservation of vitrified islets is a possible approach. However, a technological barrier is rewarming of large volumes both uniformly and rapidly to prevent ice formation due to devitrification. Here, we describe successful recovery of islets from the vitrified state using a volumetric rewarming technology called “nanowarming,” which is inductive heating of magnetic nanoparticles under an alternating magnetic field. Convective warming using a 37°C water bath as the gold standard for rewarming of vitrified samples resulted in a decrease in the viability of mouse islets in large volumes (>1 ml) owing to devitrification caused by slow warming. Nanowarming showed uniform and rapid rewarming of vitrified islets in large volumes. The viability of nanowarmed islets was significantly improved and islets transplanted into streptozotocin-induced diabetic mice successfully lowered serum glucose. The results suggest that nanowarming will lead to a breakthrough in biobanking of islets for transplantation.

    DOI： 10.1002/btm2.10416

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  82. A Sulfur Containing Melanogenesis Substrate, N-Pr-4-S-CAP as a Potential Source for Selective Chemoimmunotherapy of Malignant Melanoma Invited Reviewed

    Yasuaki Tamura, Akira Ito, Kazumasa Wakamatsu, Toshihiko Torigoe, Hiroyuki Honda, Shosuke Ito, Kowichi Jimbow

    International Journal of Molecular Sciences   Vol. 24 ( 6 ) page： 5235   2023.3

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    DOI： doi.org/10.3390/ijms24065235

  83. Novel nano-thermal ablation therapy using functionalized heat-generating nanoparticles for solid cancer treatment Invited Reviewed

    Tomio Morino, Noriyasu Kawai, Akira Ito, Takeshi Kobayashi, Takahiro Yasui

    World Journal of Cancer and Oncology Research   Vol. 2 ( 1 ) page： 29 - 46   2023.2

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： DOI: 10.31586/wjcor.2023.592

  84. Combination of magnetic hyperthermia and immunomodulators to drive complete tumor regression of poorly immunogenic melanoma

    Nishikawa Ami, Suzuki Yutaro, Kaneko Masahiro, Ito Akira

    CANCER IMMUNOLOGY IMMUNOTHERAPY     2022.12

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    Hyperthermia using magnetic nanoparticles enables tumor-specific heating and can destroy tumor tissues. This approach works as in situ vaccination with tumor antigens released from dying tumor cells. However, in situ vaccination caused by magnetic hyperthermia is often insufficient to induce complete regression of poorly immunogenic tumors surrounded by an immunosuppressive microenvironment. In this study, we explored a novel strategy for immunotherapy using magnetic hyperthermia to regress poorly immunogenic melanoma. Magnetic hyperthermia induced tumor cell death in a B16-F10 melanoma mouse model. After hyperthermia treatment, we found elevated levels of HMGB1, which is known to be released from dying cells to promote inflammation, and the proinflammatory cytokine TNF-α was increased in serum of the mice. Systemic administration of glycyrrhizin, an HMGB1 inhibitor, reduced the levels of TNF-α in serum and successfully delayed the regrowth of tumors after magnetic hyperthermia. To achieve complete tumor regression, TLR9 activation by intratumor injection of CpG was combined with systemic administration of anti-PD-1 antibody and anti-CTLA-4 antibody. The combination therapy of magnetic hyperthermia at 46°C with the immunomodulators (glycyrrhizin+CpG+anti-PD-1+anti-CTLA-4) achieved complete tumor regression in 80% of growing 5-mm B16-F10 tumors. These findings have important implications for the development of novel cancer immunotherapy using magnetic hyperthermia for poorly immunogenic tumors.

    DOI： 10.1007/s00262-022-03345-8

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  85. Molecular events in the melanogenesis cascade as novel melanoma-targeted small molecules: Principle and development Invited Reviewed

    Kazumasa Wakamatsu, Akira Ito, Yasuaki Tamura, Tokimasa Hida, Takafumi Kamiya, Toshihiko Torigoe, Hiroyuki Honda, Shosuke Ito, Kowichi Jimbow

    Cancers   Vol. 14 ( 22 ) page： 5588   2022.11

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    DOI： doi: 10.3390/cancers14225588

  86. ハイパーサーミアにおける酸化鉄ナノフラワーの開発 －ナノ構造と発熱能の関係－ Invited Reviewed

    金子真大，井藤　彰

      Vol. 38 ( 3 ) page： 63 - 64   2022.10

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  87. Induction of immunogenic cell death in murine colon cancer cells by ferrocene‐containing redox phospholipid polymers Reviewed

    Masahiro Kaneko, Akio Yamaguchi, Akira Ito

    Cancer Science   Vol. 113 ( 10 ) page： 3558 - 3565   2022.10

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  88. Nanowarming of vitrified pancreatic islets as a cryopreservation technology for transplantation Reviewed

    Taisei Wakabayashi, Masahiro Kaneko, Tomoki Nakai, Masanobu Horie, Hiroyuki Fujimoto, Maazumi Takahashi, Shota Tanoue, Akira Ito

    Bioengineering & Translational Medicine     2022.9

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    DOI： https://doi.org/10.1002/btm2.10416

  89. Immunomodulation of melanoma by chemo-thermo-immunotherapy using conjugates of melanogenesis Substrate NPrCAP and magnetite nanoparticles: a review Invited Reviewed

    Yasuaki Tamura, Akira Ito, Kazumasa Wakamatsu, Takafumi Kamiya, Toshihiko Torigoe, Hiroyuki Honda, Toshiharu Yamashita, Hisashi Uhara, Shosuke Ito, Kowichi Jimbow

    International Journal of Molecular Sciences   Vol. 23 ( 12 ) page： 6457   2022.6

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    DOI： https://doi.org/10.3390/ijms23126457

  90. Hypoxia-responsive expression of vascular endothelial growth factor for induction of angiogenesis in artificial three-dimensional tissues. Reviewed

    Shinya Masumoto, Akihiko Ono, Akira Ito, Yoshinori Kawabe, Masamichi Kamihira

    Journal of Bioscience and Bioengineering   Vol. 132 ( 4 ) page： 399 - 407   2021.10

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  91. Contractile activity of myotubes derived from human induced pluripotent stem cells: A model of duchenne muscular dystrophy Reviewed International journal

    Kantaro Yoshioka, Akira Ito, Masanobu Horie, Kazushi Ikeda, Sho Kataoka, Keiichiro Sato, Taichi Yoshigai, Hidetoshi Sakurai, Akitsu Hotta, Yoshinori Kawabe, Masamichi Kamihira

    Cells   Vol. 10 ( 10 ) page： 2556   2021.10

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    Duchenne muscular dystrophy (DMD) is a genetic disorder that results from deficiency of the dystrophin protein. In recent years, DMD pathological models have been created using induced pluripotent stem (iPS) cells derived from DMD patients. In addition, gene therapy using CRISPR-Cas9 technology to repair the dystrophin gene has been proposed as a new treatment method for DMD. However, it is not known whether the contractile function of myotubes derived from gene-repaired iPS cells can be restored. We therefore investigated the maturation of myotubes in electrical pulse stimulation culture and examined the effect of gene repair by observing the contractile behaviour of myotubes. The contraction activity of myotubes derived from dystrophin-gene repaired iPS cells was improved by electrical pulse stimulation culture. The iPS cell method used in this study for evaluating muscle contractile activity is a useful technique for analysing the mechanism of hereditary muscular disease pathogenesis and for evaluating the efficacy of new drugs and gene therapy.

    DOI： 10.3390/cells10102556

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  92. Development of a genetically modified hepatoma cell line with heat-inducible high liver function. Reviewed International journal

    Hiroyuki Kitano, Yuki Nagae, Yoshinori Kawabe, Akira Ito, Masamichi Kamihira

    Cytotechnology   Vol. 73 ( 3 ) page： 353 - 362   2021.6

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    Hepatoma cells are a promising cell source for the construction of bioartificial liver (BAL) systems owing to their high proliferative capability. However, their low liver function compared with primary hepatocytes is a major problem. In a previous study, we established a genetically modified hepatoma cell line, Hepa/8F5, in which eight liver-enriched transcription factor (LETF) genes were transduced into mouse hepatoma Hepa1-6 cells using a drug-inducible transactivator system. These cells proliferate actively under normal culture conditions, meaning that large quantities can be prepared easily. When the overexpression of the LETFs is induced by the addition of an inducer drug, cell growth stops and cell morphology changes with concomitant high expression of liver functions. However, the liver functions largely depend on the presence of the inducer drug, which must be continuously added to maintain these enhanced functions. In the present study, we attempted to modify the method of induction of LETF overexpression in Hepa/8F5 cells to remove the requirement for continual drug addition. To this end, we constructed a system in which the artificial transactivator was transcribed and amplified under the control of a heat-shock protein promoter, and introduced the system into the genome of Hepa/8F5 cells. In our modified cell line, heat-triggered LETF expression was confirmed to induce high liver function. After drug-screening of transfected cells, we established a hepatoma cell line (Hepa/HS), which exhibited high, heat-inducible liver functions. The Hepa/HS cells may represent a new cell source for hepatic studies such as the construction of BAL systems. Supplementary Information: The online version of this article (10.1007/s10616-021-00457-4) contains supplementary material, which is available to authorized users.

    DOI： 10.1007/s10616-021-00457-4

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  93. Miniaturized skeletal muscle tissue fabrication for measuring contractile activity Reviewed

    Journal of Bioscience and Bioengineering   Vol. 131 ( 4 ) page： 434 - 441   2021.4

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Journal of Bioscience and Bioengineering  

    DOI： 10.1016/j.jbiosc.2020.11.014

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  94. Magnetically triggered transgene expression system using magnetic nanoparticles Invited Reviewed

    Akira Ito

    Drug Delivery System     2021

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  95. LINE-1 vectors mediate recombinant antibody gene transfer by retrotransposition in Chinese hamster ovary cells. Reviewed

    Feiyang Zheng, Yoshinori Kawabe, Mai Murakami, Mamika Takahashi, Kyoka Nishihata, Souichiro Yoshida, Akira Ito, Masamichi Kamihira

    Biotechnology Journal   Vol. 16 ( 7 ) page： 2000620   2021

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  96. Intramyocardial Transplantation ofHuman iPS Cell–Derived CardiacSpheroids Improves Cardiac Function inHeart Failure Animals. Reviewed

    JACC: Basic to Translational Science   Vol. 36 ( 4 ) page： 101 - 108   2021

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  97. Heat dose based large tumor treatment with multiple site injections of heat-generating nanoparticles dispersible within tumor tissue Reviewed

    2. Tomio Morino, Akira Ito, Toshiki Etani, Taku Naiki, Noriyasu Kawai, Takahiro Yasui

    Thermal Medicine   Vol. 36 ( 4 ) page： 104 - 108   2020.12

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  98. Heat Dose Based Large Tumor Treatment with Multiple Site Injections of Heat-generating Nanoparticles Dispersible within Tumor Tissue

    MORINO TOMIO, ITO AKIRA, ETANI TOSHIKI, NAIKI TAKU, KAWAI NORIYASU, YASUI TAKAHIRO

    Thermal Medicine   Vol. 36 ( 4 ) page： 101 - 108   2020.12

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    Language：English   Publisher：Japanese Society for Thermal Medicine  

    <p>Solid cancer therapy based on necrosis induction with heat-generating nanoparticles has been developed in Japan. Heat was induced from intratumorally injected magnetite cationic lipid composite particles (MCL particles) by alternating magnetic field irradiations to kill cancer cells nearby located. In our previous report we have showed importance of heat dose index <i>in vivo</i> (J/cm3 tumor volume) for tumor regression when 45 mg MCL particles were injected at a single site of 1.36 cm3 tumor. Purpose of this study is to show rationale and utility of multiple site injections of MCL particles for treatment of large tumors more than 1.36 cm3.</p><p>Rat mammary tumors were induced by 7,12-dimethylbenz［a］anthracene (DMBA) and tumors in range of 2.19 ～ 3.81 cm3 were applied to treatment experiment. Treatment condition was designed to reproduce the heat generation condition of the 1.36 cm3 tumor treatment at every multiple injection site in rat mammary tumors. Tumor volume divided by number of injection sites (cm3/site) was set to close to 1.36 cm3/site and 45 mg MCL particles were administered at multiple sites to keep even spaces among injection sites. Three irradiation conditions were set to give close heat dose <i>in vivo</i> (J/cm3) of the 1.36 cm3 tumor treatment. Treatment under designed conditions resulted in complete regression of rat tumors at 21 days after the treatment, showing theoretical validity of design procedures for the multiple site injections. Novel concept of necrosed tumor volume from an injection site (cm3/site) and its actual value under a standard injection condition of 45 mg-MCL/site were described and its use in clinic was discussed.</p>

    DOI： 10.3191/thermalmed.36.101

    CiNii Research

  99. ハイパーサーミアの新展開 -基礎医学・臨床応用に関する最近の進歩- Reviewed

    近藤隆、鈴木文男、水上達治、斎藤淳一、河合憲康、井藤　彰

    放射線生物研究   Vol. 55 ( 3 ) page： 210 - 228   2020.9

  100. Magnetic heating of nanoparticles as a scalable cryopreservation technology for human induced pluripotent stem cells

    Ito Akira, Yoshioka Kantaro, Masumoto Shinya, Sato Keiichiro, Hatae Yuki, Nakai Tomoki, Yamazaki Takashi, Takahashi Masazumi, Tanoue Shota, Horie Masanobu

    SCIENTIFIC REPORTS   Vol. 10 ( 1 )   2020.8

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    DOI： 10.1038/s41598-020-70707-6

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  101. Heat Dose Index in vivo for Cancer Therapy Using Heat-generating Nanoparticles Named Magnetite Cationic Liposomes and Alternating Magnetic Field Irradiator

    MORINO TOMIO, TANOUE SHOTA, MIYATA SHUICHIRO, HIRAYAMA KOTARO, ITO AKIRA, ETANI TOSHIKI, NAIKI TAKU, KAWAI NORIYASU, YASUI TAKAHIRO

    Thermal Medicine   Vol. 36 ( 2 ) page： 47 - 58   2020.7

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    Language：English   Publisher：Japanese Society for Thermal Medicine  

    <p>Cancer clinical research using heat-generating nanoparticles named magnetite cationic liposomes (MCL) and alternating magnetic field (AMF) irradiator has been conducted. Heat generation from intratumorally injected MCL particles was triggered by AMF irradiation to kill cancer cells nearby located. Tumor temperature was monitored as index to control treatment condition but efficacy was variable from complete regression to ineffective. In order to improve efficacy, we have proposed novel index of heat dose <i>in vivo</i> (J/cm3 tumor volume). Purpose of this study was to reveal actual heat dose <i>in vivo </i>and discuss its utility as index.</p><p>In order to enable to estimate heat dose, heat generation activity of MCL particles (J/g-MCL・min) was measured under various AMF irradiation conditions by changing output power (kW) and distance from irradiation surface (mm). Treatment condition for complete regression of animal tumors with 7 mm diameter was reproduced and heat dose <i>in vivo</i> (J/cm3) was calculated by multiplying heat generation activity (J/g-MCL・min) with MCL dosage (g-MCL/cm3) and irradiation time (min). Heat dose for tumor regression was revealed around 700-850 J/cm3 in every thrice AMF irradiations of a course. Since temperature-based treatments of large tumors were reported to fall into insufficiency, revealed heat dose was applied to design treatment condition of large tumor with 13-16 mm diameter, and complete regression was achieved by a course treatment. MCL dosage of temperature-based condition was found far lower than that of heat dose. Low MCL dosages (g-MCL/cm3) would cause shortage of heat dose (J/cm3) and insufficient anticancer activity, although tumor temperature could be raised by heat transfer to monitoring sites. These results showed utility of heat dose <i>in vivo</i> as index to ensure clinical efficacy and concomitantly to make useless invasive probe for temperature monitoring. Procedure to design treatment condition and required performance of AMF irradiator were described.</p>

    DOI： 10.3191/thermalmed.36.47

    CiNii Research

  102. 若手化学工学者としての私の履歴書 Invited Reviewed

    井藤　彰

    学術の動向   Vol. 25 ( 7 ) page： 98 - 102   2020.7

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    Authorship：Lead author,　Last author,　Corresponding author   Language：Japanese  

  103. A Curriculum Vitae of Mine as a Chemical Engineer

    ITO Akira

    TRENDS IN THE SCIENCES   Vol. 25 ( 7 ) page： 7_98 - 7_102   2020.7

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    Language：Japanese   Publisher：Japan Science Support Foundation  

    DOI： 10.5363/tits.25.7_98

    CiNii Research

  104. Heat dose index in vivo for cancer therapy using heat-generating nanoparticles named magnetite cationic liposomes and alternating magnetic field irradiator Reviewed

    Tomio Morino, Shota Tanoue, Shuichiro Miyata, Kotaro Hirayama, Akira Ito, Toshiki Etani, Taku Naiki, Noriyasu Kawai, Takahiro Yasui

      Vol. 36 ( 2 ) page： 47 - 58   2020.7

  105. Novel neuromuscular junction model in 2D and 3D myotubes co-cultured with induced pluripotent stem cell-derived motor neurons. Reviewed

    Kantaro Yoshioka, Akira Ito, Yoshinori Kawabe, Masamichi Kamihira

    Journal of bioscience and bioengineering   Vol. 129 ( 4 ) page： 486 - 493   2020.4

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    Motor neurons differentiated from induced pluripotent stem (iPS) cells have attracted attention for use in the construction of drug screening systems for neuronal diseases, such as amyotrophic lateral sclerosis. However, conventional drug screening systems using 2-dimensional (2D) cultures of iPS cell-derived motor neurons often evaluate the cell survival rate, morphological changes in the cells and/or gene expression analysis, and these parameters do not always reflect the actual functions of motor neurons, i.e., the induction of muscle contractions. In the present study, we developed a neuromuscular junction model comprising motor neurons and myotubes, which were differentiated from iPS cells and C2C12 myoblasts, respectively. Using this model, the contractile activity and force generation of the myotubes via the neuromuscular junction were successfully measured in both two- and three-dimensional (3D) cell culture systems. The results suggested that this neuromuscular junction model can be used to construct a drug candidate screening system for neuronal diseases.

    DOI： 10.1016/j.jbiosc.2019.10.004

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    PubMed

  106. Targeted knock-in into the OVA locus of chicken cells using CRISPR/Cas9 system with homology-independent targeted integration. Reviewed

    Ming Shi, Yoshinori Kawabe, Akira Ito, Masamichi Kamihira

    Journal of bioscience and bioengineering   Vol. 129 ( 3 ) page： 363 - 370   2020.3

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    It is anticipated that transgenic avian species will be used as living bioreactors for the production of biopharmaceutical proteins. Precise tissue-specific expression of exogenous genes is a major challenge for the development of avian bioreactors. No robust vector is currently available for highly efficient and specific expression. In recent years, genome-editing techniques such as the CRISPR/Cas9 system have emerged as efficient and user-friendly genetic modification tools. Here, to apply the CRISPR/Cas9 system for the development of transgenic chickens, guide RNA sequences (gRNAs) of the CRISPR/Cas9 system for the ovalbumin (OVA) locus were evaluated for the oviduct-specific expression of exogenous genes. An EGFP gene expression cassette was introduced into the OVA locus of chicken DF-1 and embryonic fibroblasts using the CRISPR/Cas9 system mediated by homology-independent targeted integration. For the knock-in cells, EGFP expression was successfully induced by activation of the endogenous OVA promoter using the dCas9-VPR transactivation system. The combination of gRNAs designed around the OVA TATA box was important to induce endogenous OVA gene expression with high efficiency. These methods provide a useful tool for studies on the creation of transgenic chicken bioreactors and the activation of tissue-specific promoters.

    DOI： 10.1016/j.jbiosc.2019.09.011

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    PubMed

  107. Medium temperature independent cytotoxicity of cell-adhesive heat-generating nanoparticles named magnetite cationic liposomes and its therapeutic use Reviewed

    Tomio Morino, Hiroshi Takase, Toshiki Etani, Taku Naiki, Noriyasu Kawai, Akira Ito, Takahiro Yasui

    Thermal Medicine   Vol. 36 ( 1 ) page： 25 - 34   2020.3

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  108. Cre-Mediated Transgene Integration in Chinese Hamster Ovary Cells Using Minicircle DNA Vectors. Reviewed International journal

    Wang X, Kawabe Y, Hada T, Ito A, Kamihira M

    Biotechnology journal   Vol. 13 ( 7 ) page： e1800063   2018.7

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    Bacterial backbone sequences of conventional plasmid vectors have been reported to exhibit negative effects on transgene expression in mammalian cells, such as cytotoxicity and gene silencing. Minicircle DNA vectors can be employed to overcome these issues and to improve the transfection efficiency because of their smaller size. In this study, transgenes are integrated into the hypoxanthine phosphoribosyltransferase (hprt) locus of Chinese hamster ovary (CHO) cells by the Cre-loxP system using minicircle DNA vectors as transgene donors. The targeted transgene integration efficiency is improved 2-3-fold (≈1.4%) using minicircle DNA vectors compared with conventional plasmid vectors. Moreover, clones with expected structures after transgene integration are obtained with a high frequency. When a transgene together with bacterial sequences derived from a plasmid vector is integrated into the hprt locus, the cell growth rate and antibody titer decrease. These results indicate that minicircle DNA vectors are more suitable than conventional plasmid vectors for transgene delivery in recombinant protein production using CHO cells.

    DOI： 10.1002/biot.201800063

    PubMed

  109. Targeted knock-in of an scFv-Fc antibody gene into the hprt locus of Chinese hamster ovary cells using CRISPR/Cas9 and CRIS-PITCh systems Reviewed

    Yoshinori Kawabe, Shinya Komatsu, Shodai Komatsu, Mai Murakami, Akira Ito, Tetsushi Sakuma, Takahiro Nakamura, Takashi Yamamoto, Masamichi Kamihira

    Journal of Bioscience and Bioengineering   Vol. 125 ( 5 ) page： 599 - 605   2018.5

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Elsevier B.V.  

    Chinese hamster ovary (CHO) cells have been used as host cells for the production of pharmaceutical proteins. For the high and stable production of target proteins, the transgene should be integrated into a suitable genomic locus of host cells. Here, we generated knock-in CHO cells, in which transgene cassettes without a vector backbone sequence were integrated into the hprt locus of the CHO genome using clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 and CRISPR-mediated precise integration into target chromosome (CRIS-PITCh) systems. We investigated the efficiency of targeted knock-in of transgenes using these systems. As a practical example, we generated knock-in CHO cells producing an scFv-Fc antibody using the CRIS-PITCh system mediated by microhomology sequences for targeting. We found that the CRIS-PITCh system can facilitate targeted knock-in for CHO cell engineering.

    DOI： 10.1016/j.jbiosc.2017.12.003

    Scopus

    PubMed

  110. Characterization of genetically engineered mouse hepatoma cells with inducible liver functions by overexpression of liver-enriched transcription factors Reviewed

    Hideaki Yamamoto, Jane Marie Tonello, Takanori Sambuichi, Yoshinori Kawabe, Akira Ito, Masamichi Kamihira

    Journal of Bioscience and Bioengineering   Vol. 125 ( 1 ) page： 131 - 139   2018.1

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Elsevier B.V.  

    New cell sources for the research and therapy of organ failure could significantly alleviate the shortage of donor livers that are available to patients who suffer from liver disease. Liver carcinoma derived cells, or hepatoma cells, are the ideal cells for developing bioartificial liver systems. Such cancerous liver cells are easy to prepare in large quantities and can be maintained over long periods under standard culture conditions, unlike primary hepatocytes. However, hepatoma cells possess only a fraction of the functions of primary hepatocytes. In a previous study, by transducing cells with liver-enriched transcription factors that could be inducibly overexpressed—hepatocyte nuclear factor (HNF)1α, HNF1β, HNF3β [FOXA2], HNF4α, HNF6, CCAAT/enhancer binding protein (C/EBP)α, C/EBPβ and C/EBPγ—we created mouse hepatoma cells with high liver-specific gene expression called the Hepa/8F5 cell line. In the present study, we performed functional and genetic analyses to characterize the Hepa/8F5 cell line. Further, in three-dimensional cultures, the function of these cells improved significantly compared to parental cells. Ultimately, these cells might become a new resource that can be used in basic and applied hepatic research.

    DOI： 10.1016/j.jbiosc.2017.07.011

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  111. 温熱誘導型遺伝子発現システムの開発と応用 Invited Reviewed

    井藤　彰

    放射線生物研究   Vol. 53 ( 2 ) page： 104-114   2018

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    Authorship：Lead author   Language：Japanese  

  112. ハイパーサーミアに伴う免疫賦活のメカニズム Invited Reviewed

    井藤 彰

    放射線生物研究   Vol. 53 ( 2 ) page： 104-114   2018

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    Language：Japanese   Publishing type：Research paper (scientific journal)  

  113. Three-dimensional culture of a genetically modified hepatoma cell line using macroporous gelatin beads Reviewed

    Jane Marie Tonello, Saori Kawashima, Kazuki Sato, Yoshinori Kawabe, Akira Ito, Masamichi Kamihira

    CYTOTECHNOLOGY   Vol. 69 ( 6 ) page： 925 - 931   2017.12

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：SPRINGER  

    Hepatoma cells are a candidate cell source for bio-artificial livers. However, they exhibit reduced liver functions compared with primary hepatocytes. In our previous study, genetically engineered mouse hepatoma cells were created by transduction with vectors mediating inducible overexpression of eight liver-enriched transcription factors. Upon the induction of the liver-enriched transcription factors transduced, the cells expressed both phenotypic and genotypic liver functions at high levels. In the present study, we performed three-dimensional culture of these cells using macroporous gelatin beads. When immobilized on the macroporous gelatin beads, these cells exhibited further enhancement in liver functionality, including increased albumin secretion, ammonia removal and cytochrome P450 activity. The levels of these functions were significantly enhanced compared to monolayer culture. The method is simple and scalable, and provides highly functional cells that can be used in basic and applied fields of hepatic research.

    DOI： 10.1007/s10616-017-0117-0

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  114. Accumulative scFv-Fc antibody gene integration into the &IThprt&IT chromosomal locus of Chinese hamster ovary cells Reviewed

    Xue Wang, Yoshinori Kawabe, Risa Kato, Takeshi Hada, Akira Ito, Yoshimasa Yamana, Masako Kondo, Masamichi Kamihira

    JOURNAL OF BIOSCIENCE AND BIOENGINEERING   Vol. 124 ( 5 ) page： 583 - 590   2017.11

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：SOC BIOSCIENCE BIOENGINEERING JAPAN  

    We have previously developed an accumulative site-specific gene integration system (AGIS) using Cre-recombinase and mutated loxP sites. AGIS enables repeated transgene integration into a predetermined chromosomal site in mammalian cells. However, the process of establishing cells with multiple integrated copies of the transgene is still time-consuming. In the present study, we describe an improved version of AGIS that facilitates and accelerates the establishment of high-producer Chinese hamster ovary (CHO) cells. Two donor vectors were simultaneously introduced into the cells in a single transfection. Cells with successfully targeted transgene integration were screened based on a change in the color of the reporter fluorescent protein that they express. Repeated rounds of integration allowed the transgene copy number to be increased. As a model, an scFv-Fc antibody gene was integrated into the hprt locus of the CHO cell genome. After three rounds of integration, a high-producer CHO cell clone with six copies of the scFv-Fc gene was successfully established. scFv-Fc productivity was approximately four-fold greater than a control cell line harboring a single copy of the transgene. This newly designed AGIS procedure should facilitate the development of producer cells suitable for biopharmaceutical protein production. (C) 2017, The Society for Biotechnology, Japan. All rights reserved.

    DOI： 10.1016/j.jbiosc.2017.05.017

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  115. Hypoxia-responsive transgene expression system using RTP801 promoter and synthetic transactivator fused with oxygen-dependent degradation domain Reviewed

    Akihiko Ono, Akira Ito, Tomonaga Sato, Masaki Yamaguchi, Taiga Suzuki, Yoshinori Kawabe, Masamichi Kamihira

    JOURNAL OF BIOSCIENCE AND BIOENGINEERING   Vol. 124 ( 1 ) page： 115 - 124   2017.7

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：SOC BIOSCIENCE BIOENGINEERING JAPAN  

    Precise control of gene expression using an artificial gene circuit is a major challenge in the application of synthetic biology. Here, we designed a hypoxia-responsive transgene expression system by combining a hypoxia-inducible RTP801 promoter and a tetracycline-responsive transactivator fused with an oxygen-dependent degradation domain (TA-ODD). The reporter gene expression was highly induced by hypoxia when a transactivator-expression plasmid, pRTP801/TA-ODD, harboring a TA-ODD gene driven by the RTP801 promoter, was cotransfected with a reporter plasmid, pTRE/EGFP, harboring an EGFP gene controlled under the transactivator-responsive promoter. A stable cell line into which the expression units RTP801/TA-ODD and THE/EGFP had been introduced responded to hypoxia and expressed the reporter gene in an oxygen-concentration-dependent manner. Moreover, the cells demonstrated potential as sensors to detect hypoxic conditions in a three-dimensional tissue culture in vitro. These results indicate that the hypoxia-responsive transgene expression system is useful for constructing cell-based hypoxia detection systems. (C) 2017, The Society for Biotechnology, Japan. All rights reserved.

    DOI： 10.1016/j.jbiosc.2017.02.012

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  116. Novel therapy for pancreatic fistula using adipose-derived stem cell sheets treated with mannose Reviewed

    Kaneko Hirokazu, Kokuryo Toshio, Yokoyama Yukihiro, Yamaguchi Junpei, Yamamoto Tokunori, Shibata Rei, Gotoh Momokazu, Murohara Toyoaki, Ito Akira

    SURGERY   Vol. 161 ( 6 ) page： 1561 - 1569   2017.6

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.surg.2016.12.022

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  117. Effects of heat stimulation and L-ascorbic acid 2-phosphate supplementation on myogenic differentiation of artificial skeletal muscle tissue constructs Reviewed

    Kazushi Ikeda, Akira Ito, Masanori Sato, Shota Kanno, Yoshinori Kawabe, Masamichi Kamihira

    JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE   Vol. 11 ( 5 ) page： 1322 - 1331   2017.5

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：WILEY  

    Although skeletal muscle tissue engineering has been extensively studied, the physical forces produced by tissue-engineered skeletal muscles remain to be improved for potential clinical utility. In this study, we examined the effects of mild heat stimulation and supplementation of a L-ascorbic acid derivative, L-ascorbic acid 2-phosphate (AscP), on myoblast differentiation and physical force generation of tissue-engineered skeletal muscles. Compared with control cultures at 37 degrees C, mouse C2C12 myoblast cells cultured at 39 degrees C enhanced myotube diameter (skeletal muscle hypertrophy), whereas mild heat stimulation did not promote myotube formation (differentiation rate). Conversely, AscP supplementation resulted in an increased differentiation rate but did not induce skeletal muscle hypertrophy. Following combined treatment with mild heat stimulation and AscP supplementation, both skeletal muscle hypertrophy and differentiation rate were enhanced. Moreover, the active tension produced by the tissue-engineered skeletal muscles was improved following combined treatment. These findings indicate that tissue culture using mild heat stimulation and AscP supplementation is a promising approach to enhance the function of tissue-engineered skeletal muscles. Copyright (C) 2015 John Wiley & Sons, Ltd.

    DOI： 10.1002/term.2030

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  118. Novel therapy for pancreatic fistula using adipose-derived stem cell sheets treated with mannose. Reviewed

    Hirokazu Kaneko, Toshio Kokuryo, Yukihiro Yokoyama, Junpei Yamaguchi, Tokunori Yamamoto, Rei Shibata, Momokazu Gotoh, Toyoaki Murohara, Akira Ito, Masato Nagino

    Surgery   Vol. 161 ( 6 ) page： 1561–1569   2017

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  119. Multilayered adipose-derived regenerative cell sheets created by a novel magnetite tissue engineering method for myocardial infarction. Reviewed

    Masakazu Ishii, Rei Shibata, Yuuki Shimizu, Takashi Yamamoto, Kazuhisa Kondo, Yoko Inoue, Noriyuki Ouchi, Tohru Tanigawa, Noriyoshi Kanemura, Akira Ito, Honda Honda, Toyoaki Murohara

    International Journal of Cardiology.   Vol. 175 ( 3 ) page： 545-553   2014

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  120. TLR4 and NLRP3 inflammasome activation 1 in monocytes by N-propionyl cysteaminylphenol-maleimid-dextran (NPCMD). Reviewed

    Yu Mizote, Kazumasa Wakamatsu, Shosuke Ito, Akiko Uenaka, Yoshihiro Ohue, Koji Kurose, Midori Isobe, Akira Ito, Yasuaki Tamura, Hiroyuki Honda, Toshiharu Yamashita, Satoshi Nohara, Mikio Oka, Kowichi Jimbow, Eiichi Nakayama

    Journal of Dermatological Science   Vol. 73 ( 3 ) page： 209-215   2014

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  121. Heat-inducible gene expression system by applying alternating magnetic field to magnetic nanoparticles. Reviewed

    Masaki Yamaguchi, Akira Ito, Akihiko Ono, Yoshinori Kawabe, Masamichi Kamihira

    ACS Synthetic Biology   Vol. 3 ( 5 ) page： 273-279   2014

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  122. Enhancement of contractile force generation of artificial skeletal muscle tissues by mild and transient heat treatment. Reviewed

    Masanori Sato, Kazushi Ikeda, Shota Kanno, Akira Ito, Yoshinori Kawabe, Masamichi Kamihira

    Current Pharmaceutical Biotechnology.   Vol. 14 ( 13 ) page： 1083-1087   2014

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  123. T-cell receptor repertoires of tumor-infiltrating lymphocytes after hyperthermia using functionalized magnetite nanoparticles. Reviewed

    Akira Ito, Masaki Yamaguchi, Noriaki Okamoto, Yuji Sanematsu, Yoshinori Kawabe, Kazumasa Wakamatsu, Shosuke Ito, Hiroyuki Honda, Takeshi Kobayashi, Eiichi Nakayama, Yasuaki Tamura, Masae Okura, Toshiharu Yamashita, Kowichi Jimbow, Masamichi Kamihira

    Nanomedicine   Vol. 8 ( 6 ) page： 891-902   2013

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  124. Melanoma-targeted chemo-thermotherapy and in situ peptide immunotherapy through HSP production by using melanogenesis substrate, NPrCAP and magnetite nanoparticles. Reviewed

    Kowichi Jimbow, Yoshie Ishii-Osai, Shosuke Ito, Yasuaki Tamura, Akira Ito, Akihiro Yoneta, Takafumi Kamiya, Toshiharu Yamashita, Hiroyuki Honda, Kazumasa Wakamatsu, Katsutoshi Murase, Satoshi Nohara, Eiichi Nakayama, Takeo Hasegawa, Itsuo Yamamoto, Takeshi Kobayashi.

    Journal of Skin Cancer   Vol. 2013:742925.   2013

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    DOI： 10.1155/2013/742925

  125. Effects of B-cell lymphoma 2 gene transfer to myoblast cells on skeletal muscle tissue formation using magnetic force-based tissue engineering. Reviewed

    Masanori Sato, Akira Ito, Hirokazu Akiyama, Yoshinori Kawabe, Masamichi Kamihira

    Tissue Engineering Part A   Vol. 19 ( 1-2 ) page： 307-315   2013

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  126. Conjugation of magnetite nanoparticles with melanogenesis substrate, NPrCAP provides melanoma targeted, in situ peptide vaccine immunotherapy through HSP production by chemo-thermotherapy. Reviewed

    Kowichi Jimbow, Yasuaki Tamura, Akihiro Yoneta, Takafumi Kamiya, Ichiro Ono, Toshiharu Yamashita, Akira Ito, Hiroyuki Honda, Kazumasa Wakamatsu, Shosuke Ito, Satoshi Nohara, Eiichi Nakayama and Takeshi Kobayashi

    Journal of Biomaterials and Nanobiotechnology   Vol. 3   page： 140-153   2012

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  127. N-propionyl-4-S-cysteaminylphenol induces apoptosis in B16F1 cells and mediates tumor-specific T-cell immune responses in a mouse melanoma model. Reviewed

    Yoshie Ishii-Osai, Toshiharu Yamashita, Yasuaki Tamura, Noriyuki Sato, Akira Ito, Hiroyuki Honda, Kazumasa Wakamatsu, Shosuke Ito, Eiichi Nakayama, Masae Okura, Kowichi Jimbow

    Journal of Dermatological Science   Vol. 67   page： 51-60   2012

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  128. Accumulation gene integration into a pre-determined site using Cre/loxP. Reviewed

    Hirokazu Obayashi, Yoshinori Kawabe, Hirokazu Makitsubo, Ryoko Watanabe, Yujiro Kameyama, Shuohao Huang, Yutaka Takenouchi, Akira Ito, Masamichi Kamihira

    Journal of Bioscience and Bioengineering   Vol. 113 ( 3 ) page： 381-388   2012

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  129. Adeno-associated virus Rep-mediated targeting of integrase-defective retroviral vector DNA circles into human chromosome 19. Reviewed

    Shuohao Huang, Yoshinori Kawabe, Akira Ito, Masamichi Kamihira

    Biochemical and Biophysical Research Communications   Vol. 417 ( 1 ) page： 78-83   2012

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  130. Repeated integration of antibody genes into a pre-selected chromosomal locus of CHO cells using an accumulative site-specific gene integration system. Reviewed

    Yoshinori Kawabe, Hirokatsu Makitsubo, Yujiro Kameyama, Shuohao Huang, Akira Ito, Masamichi Kamihira

    Cytotechnology   Vol. 64 ( 3 ) page： 267-279   2012

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  131. Enhanced liver functions in mouse hepatoma cells by induced overexpression of liver-enriched transcription factors. Reviewed

    Hideaki Yamamoto, Yoshinori Kawabe, Akira Ito, Masamichi Kamihira

    Biochemical Engineering Journal   Vol. 60 ( 15 ) page： 67-73   2012

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  132. Hollow fiber bioreactor perfusion culture system for magnetic force-based skeletal muscle tissue engineering. Reviewed

    Yasunori Yamamoto, Akira Ito, Hideaki Jitsunobu, Katsuya Yamaguchi, Yoshinori Kawabe, Hiroshi Mizumoto, Masamichi Kamihira

    Journal of Chemical Engineering of Japan   Vol. 45 ( 5 ) page： 348-354   2012

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  133. Heat-inducible transgene expression system incorporating a positive feedback loop of transcriptional amplification for hyperthermia-induced gene therapy. Reviewed

    Masaki Yamaguchi, Akira Ito, Noriaki Okamoto, Yoshinori Kawabe, Masamichi Kamihira

    Journal of Bioscience and Bioengineering   Vol. 114 ( 4 ) page： 460-465   2012

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  134. Mechanism of putative neo-antigen formation from N-propionyl-4-S-cysteaminylphenol, a tyrosinase substrate, in melanoma models. Reviewed

    Shosuke Ito, Akira Nishigaki, Yasue Ishii-Osai, Makoto Ojika, Kazumasa Wakamatsu, Toshiharu Yamashita, Yasuaki Tamura, Akira Ito, Hiroyuki Honda, Eiichi Nakayama, Kouwichi Jimbow

    Biochemical Pharmacology   Vol. 84 ( 5 ) page： 646-653   2012

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  135. Heat-inducible transgene expression with transcriptional amplification mediated by a transactivator. Reviewed

    Akira Ito, Noriaki Okamoto, Masaki Yamaguchi, Yoshinori Kawabe, Masamichi Kamihira

    International Journal of Hyperthermia   Vol. 28 ( 8 ) page： 788-798   2012

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  136. Oral immunotherapy for pollen allergy using T-cell epitope-containing egg white derived from genetically manipulated chickens. Reviewed

    Yoshinori Kawabe, Yuuki Hayashida, Kensaku Numata, Shota Harada, Yoshifumi Hayashida, Akira Ito, Masamichi Kamihira

    PLoS One   Vol. 2012;7(10):e48512   2012

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    DOI： 10.1371/journal.pone.0048512

  137. A genetically engineered STO feeder system expressing E-cadherin and leukemia inhibitory factor for mouse pluripotent stem cell culture. Reviewed

    Masanobu Horie, Akira Ito, Yoshinori Kawabe, Masamichi Kamihira

    Journal of Bioprocessing and Biotechniques   Vol. S3:00   2011

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    DOI： 10.4172/2155-9821.S3-001

  138. Magnetic separation of cells from developing embryoid bodies using magnetite cationic liposomes. Reviewed

    Masanobu Horie, Akira Ito, Takeshi Maki, Yoshinori Kawabe, Masamichi Kamihira

    Journal of Bioscience and Bioengineering   Vol. 112 ( 2 ) page： 184-187   2011

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  139. Enhanced contractile force generation by artificial skeletal muscle tissues using IGF-I gene-engineered myoblast cells. Reviewed

    Masanori Sato, Akira Ito, Yoshinori Kawabe, Eiji Nagamori, Masamichi Kamihira

    Journal of Bioscience and Bioengineering   Vol. 112 ( 3 ) page： 273-278   2011

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  140. Enhanced angiogenesis by transplantation of mesenchymal stem cell sheet created by a novel magnetic tissue engineering method. Reviewed

    Masakazu Ishii, Rei Shibata, Yasushi Numaguchi, Tetsutaro Kito, Hirohiko Suzuki, Kazunori Shimizu, Akira Ito, Hiroyuki Honda, Toyoaki Murohara

    Arteriosclerosis Thrombosis and Vascular Biology   Vol. 31 ( 10 ) page： 2210-2215   2011

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  141. Functional evaluation of artificial skeletal muscle tissue constructs fabricated by a magnetic force-based tissue engineering technique. Reviewed

    Yasunori Yamamoto, Akira Ito, Hideaki Fujita, Eiji Nagamori, Yoshinori Kawabe, Masamichi Kamihira

    Tissue Engineering Part A   Vol. 17 ( 1-2 ) page： 107-114   2011

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  142. Melanogenesis exploitation and melanoma nanomedicine: utilization of melanogenesis substrate, NPrCAP for exploiting melanoma-targeting drug and its conjugation with magnetite nanoparticles for developing melanoma chemo-thermo-immunotherapy. Reviewed

    Kowichi Jimbow, Tomoaki Takada, Yoshie Osai, Panakkezhum D Thomas, Makito Sato, Akiko Sato, Takafumi Kamiya, Ichiro Ono, Yasuaki Tamura, Noriyuki Sato, Atsushi Miyamoto, Akira Ito, Hiroyuki Honda, Kazumasa Wakamatsu, Shosuke Ito, Toshiharu Yamashita, Eiichi Nakayama, Takeshi Kobayashi

    The Open Conference Proceedings Journal   Vol. 2   page： 5-16   2011

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  143. Tissue engineering using magnetite nanoparticles. Invited Reviewed

    Akira Ito, Masamichi Kamihira

    Progress in Molecular Biology and Translational Science   Vol. 104   page： 355-395   2011

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  144. Cre recombinase mediated site-specific modification of a cellular genome using an integrase-defective retroviral vector. Reviewed

    Shuohao Huang, Yoshinori Kawabe, Akira Ito, Masamichi Kamihira

    Biotechnology and Bioengineering   Vol. 107 ( 4 ) page： 717-729   2011

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  145. E-cadherin gene-engineered feeder systems for supporting undifferentiated growth of mouse embryonic stem cells. Reviewed

    Masanobu Horie, Akira Ito, Takehiko Kiyohara, Yoshinori Kawabe, Masamichi Kamihira

    Journal of Bioscience and Bioengineering   Vol. 110 ( 5 ) page： 582-587   2010

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  146. Cell-pattering using poly(ethylene glycol)-modified magnetite nanoparticles. Reviewed

    Hirokazu Akiyama, Akira Ito, Yoshinori Kawabe, Masamichi Kamihira

    Journal of Biomedical Materials Research: Part A   Vol. 92A ( 3 ) page： 1123-1130   2010

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  147. Construction of cardiac tissue rings using a magnetic tissue fabrication technique. Reviewed

    Hirokazu Akiyama, Akira Ito, Masanori Sato, Yoshinori Kawabe, Masamichi Kamihira

    International Journal of Molecular Sciences   Vol. 11 ( 8 ) page： 2910-2920   2010

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  148. Production of recombinant human erythropoietin/Fc fusion protein by genetically manipulated chickens. Reviewed

    Carlos Alberto Penno, Yoshinori Kawabe, Akira Ito, Masamichi Kamihira

    Transgenic Research   Vol. 19 ( 2 ) page： 187-195   2010

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  149. An accumulative site-specific gene integration system using Cre recombinase-mediated cassette exchange. Reviewed

    Yujiro Kameyama, Yoshinori Kawabe, Akira Ito, Masamichi Kamihira

    Biotechnology and Bioengineering   Vol. 105 ( 6 ) page： 1106-1114   2010

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  150. Transfer of gene to human retinal pigment epithelial cells using magnetite cationic liposomes. Reviewed

    Yasuki Fujii, Shu Kachi, Tamayo Kawasumi, Hiroyuki Honda, Akira Ito, Hiroko Terasaki

    British Journal of Ophthalmology   Vol. 94 ( 8 ) page： 1074-1077   2010

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  151. Fabrication of scaffold-free contractile skeletal muscle tissue using magnetite-incorporated myogenic C2C12 cells. Reviewed

    Hideaki Fujita, Kazunori Shimizu, Yasunori Yamamoto, Akira Ito, Masamichi Kamihira, Eiji Nagamori

    Journal of Tissue Engineering and Regenerative Medicine   Vol. 4 ( 6 ) page： 437-443   2010

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  152. Melanoma-targeted chemo-thermo-immuno (CTI)-therapy using N-propionyl-4-S-cysteaminylphenol-magnetite nanoparticles elicits CTL response via heat shock protein-peptide complex release. Reviewed

    Akiko Sato, Yasuaki Tamura, Noriyuki Sato, Toshiharu Yamashita, Tomoaki Takada, Makito Sato, Yasue Osai, Masae Ohkura, Ichiro Ono, Akira Ito, Hiroyuki Honda, Kazumasa Wakamatsu, Shosuke Ito, Kowichi Jimbow

    Cancer Science   Vol. 101 ( 9 ) page： 1939-1946   2010

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  153. Preparation of artificial skeletal muscle tissues by a magnetic force-based tissue engineering technique. Reviewed

    Yasunori Yamamoto, Akira Ito, Masahiro Kato, Yoshinori Kawabe, Kazunori Shimizu, Hideaki Fujita, Eiji Nagamori, Masamichi Kamihira

    Journal of Bioscience and Bioengineering   Vol. 108 ( 6 ) page： 538-543   2009

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  154. Magnetic concentration of a retroviral vector using magnetite cationic liposomes. Reviewed

    Akira Ito, Tetsuya Takahashi, Yujiro Kameyama, Yoshinori Kawabe, Masamichi Kamihira

    Tissue Engineering Part C: Methods   Vol. 15 ( 1 ) page： 57-64   2009

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  155. Fabrication of complex three-dimensional tissue architectures using a magnetic force-based cell patterning technique. Reviewed

    Hirokazu Akiyama, Akira Ito, Yoshinori Kawabe, Masamichi Kamihira

    Biomedical Microdevices   Vol. 11 ( 4 ) page： 713-721   2009

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  156. Human beta defensin-3 engineered keratinocyte sheets constructed by a magnetic force-based tissue engineering technique. Reviewed

    Akira Ito, Tetsuya Takahashi, Yoshinori Kawabe, Masamichi Kamihira

    Journal of Bioscience and Bioengineering   Vol. 108 ( 3 ) page： 244-247   2009

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  157. Magnetic separation of cells in co-culture systems using magnetite cationic liposomes. Reviewed

    Akira Ito, Hideaki Jitsunobu, Yoshinori Kawabe, Hiroyuki Ijima, Masamichi Kamihira

    Tissue Engineering Part C: Methods   Vol. 15 ( 3 ) page： 413-423   2009

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  158. N-propionyl-cysteaminylphenol-magnetite conjugate (NPrCAP/M) is a novel nanoparticle for the targeted growth suppression of melanoma cells. Reviewed

    Makito Sato, Toshiharu Yamashita, Masae Ohkura, Akiko Sato, Tomoaki Takada, Hidenobu Matsusaka, Ichiro Ono, Yasuaki Tamura, Noriyuki Sato, Yasushi Sasaki, Takashi Tokino, Akira Ito, Hiroyuki Honda, Kazumasa Wakamatsu, Shosuke Ito, Kowichi Jimbow

    Journal of Investigative Dermatology   Vol. 129 ( 9 ) page： 2233-224   2009

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  159. Growth inhibition of re-challenge B16 melanoma transplant by conjugates of melanogenesis substrate and magnetite nanoparticles as the basis for developing melanoma-targeted chemo-thermo-immunotherapy. Reviewed

    Tomoaki Takada, Toshiharu Yamashita, Makito Sato, Akiko Sato, Ichiro Ono, Yasuaki Tamura, Noriyuki Sato, Atsushi Miyamoto, Akira Ito, Hiroyuki Honda, Kazumasa Wakamatsu, Shosuke Ito, Kowichi Jimbow

    Journal of Biomedicine and Biotechnology   Vol. 2009:457936   2009

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    DOI： 10.1155/2009/457936

  160. Antibody-dependent gene transduction using gammaretroviral and lentiviral vectors pseudotyped with chimeric vesicular stomatitis virus glycoprotein. Reviewed

    Yujiro Kameyama, Yoshinori Kawabe, Akira Ito, Masamichi Kamihira

    Journal of Virological Methods   Vol. 153 ( 1 ) page： 49-54   2008

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  161. Construction of 3D tissue-like structure using functional magnetite nanoparticles. Invited Reviewed

    Akira Ito, Hiroyuki Honda, Masamichi Kamihira

    Yakugaku Zassi   Vol. 128 ( 1 ) page： 21-29   2008

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    Authorship：Lead author   Language：Japanese   Publishing type：Research paper (scientific journal)  

  162. Intracellular hyperthermia using magnetic nanoparticles: A novel method for hyperthermia clinical applications. Reviewed

    Akira Ito, Takeshi Kobayashi

    Thermal Medicine   Vol. 24 ( 4 ) page： 113-129   2008

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  163. Cell culture arrays using magnetic force-based cell patterning for dynamic single cell analysis. Reviewed

    Kosuke Ino, Mina Okochi, Nao Konishi, Masahiro Nakatochi, Rentaro Imai, Mitsuhiro Shikida, Akira Ito, Hiroyuki Honda

    Lab on a Chip   Vol. 8 ( 1 ) page： 134-142   2008

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  164. Plasmid DNA transfection using magnetite cationic liposomes for construction of multilayered gene-engineered cell sheet. Reviewed

    Kosuke Ino, Tamayo Kawasumi, Akira Ito, Hiroyuki Honda

    Biotechnology and Bioengineering   Vol. 100 ( 1 ) page： 168-176   2008

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  165. Identification of HLA-A24-restricted epitopes with high affinity to Hsp70 using peptide array. Reviewed

    Mina Okochi, Hiroki Hayashi, Akira Ito, Ryuji Kato, Yasuaki Tamura, Noriyuki Sato, Hiroyuki Honda

    Journal of Bioscience and Bioengineering   Vol. 105 ( 3 ) page： 198-203   2008

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  166. Effects of heat therapy using magnetic nanoparticles conjugated with cationic liposomes on prostate tumor in bone. Reviewed

    Noriyasu Kawai, Mitsuru Futakuchi, Tatsuro Yoshida, Akira Ito, Shinya Sato, Taku Naiki, Hiroyuki Honda, Tomoyuki Shirai, Kenjiro Kohri

    Prostate   Vol. 68 ( 7 ) page： 784-792   2008

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  167. Enhancement of cell function through heterotypic cell-cell interactions using E-cadherin-expressing NIH3T3 cells. Reviewed

    Akira Ito, Takehiko Kiyohara, Yoshinori Kawabe, Hiroyuki Ijima, Masamichi Kamihira

    Journal of Bioscience and Bioengineering   Vol. 105 ( 6 ) page： 679-682   2008

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  168. 4-S-cysteaminylphenol-loaded magnetite cationic liposomes for combination therapy of hyperthermia with chemotherapy against malignant melanoma. Reviewed

    Akira Ito, Masatake Fujioka, Tatsuro Yoshida, Kazumasa Wakamatsu, Shosuke Ito, Toshiharu Yamashita, Kowichi Jimbow, Hiroyuki Honda

    Cancer Science   Vol. 98 ( 3 ) page： 424-430   2007

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  169. Effective cell-seeding using magnetite nanoparticles and magnetic force onto decellularized blood vessels for vascular tissue engineering. Reviewed

    Kazunori Shimizu, Akira Ito, Manabu Arinobe, Yosuke Murase, Yoshihisa Iwata, Yuji Narita, Hideaki Kagami, Minoru Ueda, Hiroyuki Honda

    Journal of Bioscience and Bioengineering   Vol. 103 ( 5 ) page： 472-478   2007

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  170. Cell patterning using magnetite nanoparticles and magnetic force. Reviewed

    Kosuke Ino, Akira Ito, Hiroyuki Honda

    Biotechnology and Bioengineering   Vol. 97 ( 5 ) page： 1309-1317   2007

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  171. Incorporation of capillary-like structures into dermal cell sheets constructed by magnetic force-based tissue engineering. Reviewed

    Kosuke Ino, Akira Ito, Hirohito Kumazawa, Hideaki Kagami, Minoru Ueda, Hiroyuki Honda

    Journal of Chemical Engineering of Japan   Vol. 40 ( 1 ) page： 51-58   2007

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  172. Construction of multilayered cardiomyocyte sheets using magnetite nanoparticles and magnetic force. Reviewed

    Kazunori Shimizu, Akira Ito, Jong-Kook Lee, Tatsuro Yoshida, Keiko Miwa, Hisaaki Ishiguro, Yasushi Numaguchi, Toyoaki Murohara, Itsuo Kodama, Hiroyuki Honda

    Biotechnology and Bioengineering   Vol. 96 ( 4 ) page： 803-809   2007

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  173. Heat immunotherapy with heat shock protein expression by hyperthermia using magnetite nanoparticles. Invited Reviewed

    Akira Ito, Takeshi Kobayashi, Hiroyuki Honda

    Annals of Cancer Research and Therapy   Vol. 15 ( 2 ) page： 27-34   2007

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  174. Bone tissue engineering with human mesenchymal stem cell sheets constructed using magnetite nanoparticles and magnetic force. Reviewed

    Kazunori Shimizu, Akira Ito, Takuro Yoshida, Yoichi Yamada, Minoru Ueda, Hiroyuki Honda

    Journal of Biomedical Materials Research   Vol. 82 ( 2 ) page： 471-480   2007

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  175. Mag-seeding of rat bone marrow cells into porous hydroxyapatite scaffolds for bone tissue engineering. Reviewed

    Kazunori Shimizu, Akira Ito, Hiroyuki Honda

    Journal of Bioscience and Bioengineering   Vol. 104 ( 3 ) page： 171-177   2007

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  176. Magnetic force-based cell patterning using RGD peptide-conjugated magnetite cationic liposomes. Reviewed

    Akira Ito, Hirokazu Akiyama, Yoshinori Kawabe, Masamichi Kamihira

    Journal of Bioscience and Bioengineering   Vol. 104 ( 4 ) page： 288-293   2007

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  177. Construction of heterotypic cell sheets by magnetic force-based 3-D co-culture of HepG2 and NIH3T3 cells. Reviewed

    Akira Ito, Hideaki Jitsunobu, Yoshinori Kawabe, Masamichi Kamihira

    Journal of Bioscience and Bioengineering   Vol. 104 ( 5 ) page： 371-378   2007

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  178. Application of an ultra water-repellent surface to cell culture. Reviewed

    Kosuke Ino, Akira Ito, Yunying Wu, Nagahiro Saito, Eri Hibino, Osamu Takai, Hiroyuki Honda

    Journal of Bioscience and Bioengineering   Vol. 104 ( 5 ) page： 420-423   2007

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  179. Cancer immunotherapy based on intracellular hyperthermia using magnetite nanoparticles: A novel concept of heat-controlled necrosis with heat shock protein expression. Invited Reviewed

    Akira Ito, Hiroyuki Honda, Takeshi Kobayashi

    Cancer Immunology Immunotherapy   Vol. 55 ( 3 ) page： 320-328   2006

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  180. Cluster analysis and gene expression profiles: a cDNA microarray system-based comparison between human dental pulp stem cells (hDPSCs) and human mesenchymal stem cells (hMSCs) for tissue engineering cell therapy. Reviewed

    Yoichi Yamada, Atsushi Fujimoto, Akira Ito, Ryoko Yoshimi, Minoru Ueda

    Biomaterials   Vol. 27 ( 20 ) page： 3766-3781   2006

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  181. Complete regression of experimental prostate cancer in nude mice by repeated hyperthermia using magnetite cationic liposomes and a newly developed solenoid containing a ferrite core. Reviewed

    Noriyasu Kawai, Akira Ito, Yoko Nakahara, Hiroyuki Honda, Takeshi Kobayashi, Mitsuru Futakuchi, T Shirai, K. Tozawa, K. Kohri

    Prostate   Vol. 66 ( 7 ) page： 718-727   2006

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  182. Enhanced cell-seeding into 3D porous scaffolds by use of magnetite nanoparticles. Reviewed

    Kazunori Shimizu, Akira Ito, Hiroyuki Honda

    Journal of Biomedical Materials Research   Vol. 77 ( 2 ) page： 265-272   2006

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  183. Construction and delivery of tissue-engineering human retinal pigment epithelial cell sheets using magnetite nanoparticles and magnetic force. Reviewed

    Akira Ito, Eri Hibino, Hiroyuki Honda, Chiaki Kobayashi, Hiroko Terasaki, Hideaki Kagami, Minoru Ueda, Takeshi Kobayashi

    Tissue Engineering   Vol. 11 ( 3-4 ) page： 489-496   2005

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  184. A mechanism of antitumor immunity induced by hyperthermia Invited Reviewed

    Akira Ito, Takeshi Kobayashi, Hiroyuki Honda

    Japanese Journal of Hyperthermic Oncology   Vol. 21 ( 1 ) page： 1-11   2005

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  185. Medical application of functionalized magnetic nanoparticles. Invited Reviewed

    Akira Ito, Masashige Shinkai, Hiroyuki Honda, Takeshi Kobayashi

    Journal of Bioscience and Bioengineering   Vol. 100 ( 1 ) page： 1-11   2005

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  186. Screening of cytokines to enhance vaccine effects of heat shock protein 70-rich tumor cell lysate. Reviewed

    Akira Ito, Masatake Fujioka, Koji Tanaka, Takeshi Kobayashi, Hiroyuki Honda

    Journal of Bioscience and Bioengineering   Vol. 100 ( 1 ) page： 36-42   2005

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  187. Complete regression of hereditary melanoma in a mouse model by repeated hyperthermia using magnetite cationic liposomes. Reviewed

    Akira Ito, Yoko Nakahara, Masatake Fujioka, Takeshi Kobayashi, Kozue Takeda, Izumi Nakashima, Hiroyuki Honda

    Japanese Journal of Hyperthermic Oncology   Vol. 21 ( 3 ) page： 179-189   2005

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  188. Heat immunotherapy using magnetic nanoparticles and dendritic cells for T-lymphoma. Reviewed

    Koichi Tanaka, Akira Ito, Takeshi Kobayashi, Tatsuyoshi Kawamura, Shinji Shimada, Kazuhiko Matumoto, Toshiaki Saida, Hiroyuki Honda

    Journal of Bioscience and Bioengineering   Vol. 100 ( 1 ) page： 112-115   2005

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  189. Novel methodology for fabrication of tissue-engineered tubular constructs using magnetite nanoparticles and magnetic force. Reviewed

    Akira Ito, Kosuke Ino, Masao Hayashida, Takeshi Kobayashi, Hiroshi Matsumura, Hideaki Kagami, Minoru Ueda, Hiroyuki Honda

    Tissue Engineering   Vol. 11 ( 9-10 ) page： 1553-1561   2005

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  190. Anticancer effect of hyperthermia on prostate cancer mediated by magnetite cationic liposomes and immune-response induction in transplanted syngeneic rats. Reviewed

    Noriyasu Kawai, Akira Ito, Yoko Nakahara, Mitsuru Futakuchi, Tomoyuki Shirai, Hiroyuki Honda, Takeshi Kobayashi, Kenjiro Kohri

    Prostate   Vol. 64 ( 4 ) page： 373-381   2005

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  191. Intratumoral injection of immature dendritic cells enhances antitumor effect of hyperthermia using magnetic nanoparticles. Reviewed

    Koji Tanaka, Akira Ito, Takeshi Kobayashi, Tatsuyoshi Kawamura, Shinji Shimada, Kazuhiko Matsumoto, Toshiaki Saida, Hiroyuki Honda

    International Journal of Cancer   Vol. 116 ( 4 ) page： 624-633   2005

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  192. Magnetic force-based mesenchymal stem cell expansion using antibody-conjugated magnetoliposomes. Reviewed

    Akira Ito, Eri Hibino, Kazunori Shimizu, Takeshi Kobayashi, Yoichi Yamada, Hideharu Hibi, Minoru Ueda, Hiroyuki Honda

    Journal of Biomedical Materials Research   Vol. 75 ( 2 ) page： 320-327   2005

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  193. The effect of RGD peptide-conjugated magnetite cationic liposomes on cell growth and cell sheet harvesting. Reviewed

    Akira Ito, Kosuke Ino, Takeshi Kobayashi, Hiroyuki Honda

    Biomaterials   Vol. 26 ( 31 ) page： 6185-6193   2005

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  194. Functional magnetic particles for medical application. Invited Reviewed

    Masashige Shinkai, Akira Ito

    Advance of Biochemical Engineering and Biotechnology   Vol. 91   page： 191-220   2004

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  195. Construction and harvest of multilayered keratinocyte sheets using magnetite nanoparticles and magnetic force. Reviewed

    Akira Ito, Masao Hayashida, Hiroyuki Honda, Kenichiro Hata, Hideaki Kagami, Minoru Ueda, Takeshi Kobayashi

    Tissue Engineering   Vol. 10 ( 5-6 ) page： 873-880   2004

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  196. A new methodology of mesenchymal stem cell expansion using magnetic nanoparticles. Reviewed

    Akira Ito, Eri Hibino, Hiroyuki Honda, Kenichiro Hata, Hideaki Kagami, Minoru Ueda, Takeshi Kobayashi

    Biochemical Engineering Journal   Vol. 20 ( 2-3 ) page： 119-125   2004

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  197. Local hyperthermia enhances thrombosis in aneurysms containing platinum coils. Reviewed

    Osamu Suzuki, Shigeru Miyachi, Takeshi Okamoto, Akira Ito, Masashige Shinkai, Hiroyuki Honda, Takeshi Kobayashi, Makoto Neguro, Jun Yoshida

    Interventional Neuroradiology   Vol. 10 ( 3 ) page： 203-211   2004

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  198. Magnetite nanoparticle-loaded anti-HER2 immunoliposomes for combination of antibody therapy with hyperthermia. Reviewed

    Akira Ito, Yuko Kuga, Hiroyuki Honda, Hiroyuki Kikkawa, Atsushi Horiuchi, Yuji Watanabe, Takeshi Kobayashi

    Cancer Letters   Vol. 212 ( 2 ) page： 167-175   2004

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  199. Antitumor effects of combined therapy of recombinant heat shock protein 70 and hyperthermia using magnetic nanoparticles in an experimental subcutaneous murine melanoma. Reviewed

    Akira Ito, Fumiko Matsuoka, Hiroyuki Honda, Takeshi Kobayashi

    Cancer Immunology Immunotherapy   Vol. 53 ( 1 ) page： 26-32   2004

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  200. Tumor regression by combined immunotherapy and hyperthermia using magnetic nanoparticles in an experimental subcutaneous murine melanoma. Reviewed

    Akira Ito, Koji Tanaka, Kazuyoshi Kondo, Masashige Shinkai, Hiroyuki Honda, Kazuhiko Matsumoto, Toshiaki Saida, Takeshi Kobayashi

    Cancer Science   Vol. 94 ( 3 ) page： 308-313   2003

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  201. Tumor regression by combined immunotherapy and hyperthermia using magnetic nanoparticles in an experimental subcutaneous murine melanoma. Reviewed

    Akira Ito, Koji Tanaka, Kazuyoshi Kondo, Masashige Shinkai, Hiroyuki Honda, Kazuhiko Matsumoto, Toshiaki Saida, Takeshi Kobayashi

    Cancer Science   Vol. 94 ( 3 ) page： 308-313   2003

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    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

  202. Time course of biodistribution and heat generation of magnetite cationic liposomes in mouse model. Reviewed

    Akira Ito, Yoko Nakahara, Koji Tanaka, Yuko Kuga, Hiroyuki Honda, Takeshi Kobayashi

    Japanese Journal of Hyperthermic Oncology   Vol. 19 ( 3 ) page： 151-159   2003

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    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

  203. Heat shock protein 70 expression induces an antitumor immunity during intracellular hyperthermia using magnetite nanoparticles. Reviewed

    Akira Ito, Masashige Shinkai, Hiroyuki Honda, Kazuhiro Yoshikawa, Shinsuke Saga, Toshihiko Wakabayashi, Jun Yoshida, Takeshi Kobayashi

    Cancer Immunology Immunotherapy   Vol. 52 ( 2 ) page： 80-88   2003

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    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

  204. Proliferation and stratification of keratinocyte on cultured amniotic epithelial cells for tissue engineering. Reviewed

    Akira Ito, Yohei Takizawa, Masashige Shinkai, Hiroyuki Honda, Kenichiro Hata, Minoru Ueda, Naohiko Kuno, Atsuo Itakura, Takeshi Kobayashi

    Journal of Bioscience and Bioengineering   Vol. 95 ( 6 ) page： 589-593   2003

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    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

  205. Selective hyperthermia using magnetoliposomes to target cervical lymph node metastasis in a rabbit tongue tumor model. Reviewed

    Shigeaki Hamaguchi, Iwai Tohnai, Akira Ito, Kenji Mitsudo, Toshio Shigetomi, Masafumi Ito, Hiroyuki Honda, Takeshi Kobayashi, Minoru Ueda

    Cancer Science   Vol. 94 ( 9 ) page： 834-839   2003

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    Language：English   Publishing type：Research paper (scientific journal)  

  206. ハイパーサーミアに伴う免疫賦活のメカニズム Invited Reviewed

    井藤　彰, 本多裕之, 小林　猛

    放射線生物研究   Vol. 38 ( 4 ) page： 405-420   2003

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    Authorship：Lead author   Language：Japanese   Publishing type：Research paper (scientific journal)  

  207. Heat shock protein 70 gene therapy combined with hyperthermia using magnetic nanoparticles. Reviewed

    Akira Ito, Fumiko Matsuoka, Hiroyuki Honda, Takeshi Kobayashi

    Cancer Gene Therapy   Vol. 10 ( 12 ) page： 918-925   2003

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    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

▼display all

To the head of Papers.▲

Books 32

  1. 磁力を用いた細胞操作

    井藤　彰（ Role： Sole author）

    三次元ティッシュエンジニアリング-細胞の培養・操作・組織化から品質管理、脱細胞化まで  2015 

    　More details

    Language：Japanese

  2. 磁力を用いた細胞3次元組織化

    井藤　彰（ Role： Sole author）

    細胞の3次元組織化―その最先端技術と材料技術  2014 

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    Language：Japanese

  3. Construction of functional cardiovascular tissues using magnetic nanoparticles.

    Jong-Kook Lee, Akira Ito, Hiroyuki Honda（ Role： Joint author）

    Cardiac Regeneration Using Stem Cells  2013 

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    Language：English

  4. Tissue engineering using magnetite nanoparticles.

    Akira Ito, Masamichi Kamihira（ Role： Joint author）

    Prog Mol Biol Transl Sci.   2011 

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    Language：English

  5. Magnetic nanoparticles for tissue engineering

    Akira Ito, Hiroyuki Honda（ Role： Joint author）

    Nanotechnologies for Tissue, Cell and Organ Engineering  2006 

    　More details

    Language：English

  6. 磁性微粒子が拓く新しい医療

    井藤　彰、本多裕之（ Role： Joint author）

    エコマテリアルハンドブック  2005 

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    Language：Japanese

  7. リポソームによるガンの温熱療法の開発

    井藤　彰、本多裕之、小林　猛（ Role： Joint author）

    リポソーム応用の新展開～人工細胞の開発に向けて～  2005 

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    Language：Japanese

  8. 機能性磁性ナノ粒子を用いるガンの温熱免疫療法の開発

    井藤　彰、小林　猛、本多裕之（ Role： Joint author）

    ナノ粒子・マイクロ粒子の最先端技術  2005 

    　More details

    Language：Japanese

  9. 磁性微粒子を用いたガンの温熱療法

    井藤　彰、小林　猛、本多裕之（ Role： Joint author）

    最近の化学工学５６　先端医療における化学工学  2004 

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    Language：Japanese

  10. マグネトリポソームを用いた温熱免疫療法

    若林俊彦、中原紀元、吉田　純、井藤　彰、本多裕之、小林　猛（ Role： Joint author）

    先端医療シリーズ18・脳神経外科：脳腫瘍の最新医療  2003 

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    Language：Japanese

  11. 磁性微粒子を用いた細胞内温熱療法の検討

    若林俊彦、中原紀元、国本圭市、藤井正純、波多野寿、大野貴也、吉田純、井藤彰、新海政重、本多裕之、小林猛、竹村篤人（ Role： Joint author）

    ポストシークエンス時代における脳腫瘍の研究と治療  2002 

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    Language：Japanese

  12. 機能性磁性ナノ粒子の開発と医療分野への応用

    金子真大、井藤　彰（ Role： Contributor）

    化学工業社  2021.4 

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    Total pages：7   Responsible for pages：7   Language：Japanese Book type：Scholarly book

  13. 金属ナノ粒子

    金子真大、井藤　彰（ Role： Joint author ,  機能性磁性ナノ粒子の合成と医療分野への応用）

    サイエンス＆テクノロジー  2021 

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    Language：Japanese Book type：Scholarly book

  14. Magnetite nanoparticles: Functionalization and manufacturing of pluripotent stem cells.

    Masanobu Horie, Anuj Tripathi, Akira Ito, Yoshinori Kawabe, Masamichi Kamihira（ Role： Joint author）

    Advanced Structured Materials  2017 

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    Language：English

  15. Magnetite nanoparticles: Functionalization and manufacturing of pluripotent stem cells.

    Masanobu Horie, Anuj Tripathi, Akira Ito, Yoshinori Kawabe, Masamichi Kamihira（ Role： Joint author）

    Advanced Structured Materials  2017 

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    Responsible for pages：363-383   Language：English

  16. 細胞操作

    李 鐘国, 井藤　彰, 本多裕之（ Role： Joint author）

    磁気便覧  2016 

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    Language：Japanese

  17. 磁気細胞操作技術による3次元細胞組織構築

    井藤　彰（ Role： Sole author）

    ３次元細胞システム設計論  2016 

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    Language：Japanese

  18. Chapter 1 Skin

    K. Kito, H. Kagami, C. Kobayashi, H. Terasaki, Y. Miyata, K. Okada, A. Fujimoto, K. Hata, Y. Tomita, A. Ito, M. Hayashida, H. Honda, T. Kobayashi, Y. Sugimura, S. Torii, K. Horie, Y. Hibino, K. Toriyama, Y. Sumi, H. Mizuno, A. Niimi, N. Emi, A. Abe, I. Takahashi, T. Kojima, H. Saito, M. Ueda（ Role： Joint author）

    Applied Tissue Engineering  2011 

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    Language：English

  19. 磁性ナノ粒子を用いた新しいガン治療法の開発

    小林　猛, 井藤　彰, 本多裕之（ Role： Joint author）

    ナノ粒子・マイクロ粒子の調製と応用技術  2010 

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    Language：Japanese

  20. 細胞の磁気操作による三次元組織の構築

    井藤　彰, 本多裕之, 上平正道（ Role： Joint author）

    細胞分離・操作技術の最前線  2008 

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    Language：Japanese

  21. 免疫との併用

    井藤　彰（ Role： Sole author）

    ハイパーサーミア がん温熱療法ガイドブック  2008 

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    Language：Japanese

  22. Functionalized magnetic nanoparticles for tissue engineering

    Akira Ito, Hiroyuki Honda（ Role： Joint author）

    New Research on Biomaterials  2007 

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    Language：English

  23. Magnetic nanoparticles for tissue engineering

    Akira Ito, Hiroyuki Honda（ Role： Joint author）

    Nanotechnologies for Tissue, Cell and Organ Engineering  2006 

    　More details

    Language：English

  24. 磁性微粒子が拓く新しい医療

    井藤　彰, 本多裕之（ Role： Joint author）

    エコマテリアルハンドブック  2006 

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    Language：Japanese

  25. 細胞の磁気ラベル・磁気誘導を用いた組織構築

    本多裕之, 井藤　彰, 清水一憲, 伊野浩介（ Role： Joint author）

    一細胞定量解析の最前線 –ライフサーベイヤ構築に向けて-  2006 

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    Language：Japanese

  26. 再生医療への応用技術

    井藤　彰, 本多裕之（ Role： Joint author）

    磁性ビーズのバイオ・環境技術への応用展開  2006 

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    Language：Japanese

  27. リポソームによるガンの温熱療法の開発

    井藤　彰, 小林　猛, 本多裕之（ Role： Joint author）

    リポソーム応用の新展開～人工細胞の開発に向けて～  2005 

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    Language：Japanese

  28. 抗体結合型マグネトリポソームを用いた間葉系幹細胞の濃縮培養法

    清水一憲, 井藤　彰, 日比野恵里, 小林　猛, 山田陽一, 日比英晴, 上田　実, 本多裕之（ Role： Joint author）

    化学工学シンポジウムシリーズ「診断・治療システムと化学工学」  2005 

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    Language：Japanese

  29. 磁性ナノ粒子を用いた管状組織の構築

    伊野浩介, 井藤　彰, 林田真生, 小林　猛, 松沼　寛, 各務秀明, 上田　実, 本多裕之（ Role： Joint author）

    化学工学シンポジウムシリーズ「診断・治療システムと化学工学」  2005 

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    Language：Japanese

  30. 磁性微粒子を用いたガンの温熱療法

    井藤　彰, 小林　猛, 本多裕之（ Role： Joint author）

    最近の化学工学56　先端医療における化学工学  2004 

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    Language：Japanese

  31. 機能性磁性ナノ粒子を用いるガンの温熱免疫療法の開発

    井藤　彰, 小林　猛, 本多裕之（ Role： Joint author）

    ナノ粒子・マイクロ粒子の最先端技術  2004 

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    Language：Japanese

  32. マグネトリポソームを用いた温熱免疫療法

    若林俊彦, 中原紀元, 吉田　純, 井藤　彰, 本多裕之, 小林　猛（ Role： Joint author）

    先端医療シリーズ18・脳神経外科：脳腫瘍の最新医療、先端医療技術研究所  2003 

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    Language：Japanese

▼display all

To the head of Books.▲

MISC 25

  1. 磁性ナノ粒子が拓く医療技術 Invited

    井藤　彰

    PRESSe   Vol. 48 ( 17 ) page： 17 - 17   2022.12

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    Authorship：Lead author   Language：Japanese   Publishing type：Article, review, commentary, editorial, etc. (bulletin of university, research institution)  

  2. Study on the Relationship between Structure and Function of Iron Oxide Nanoflowers for Efficient Hyperthermia Reviewed

    MASAHIRO KANEKO, AKIRA ITO

    Thermal Medicine   Vol. 38 ( 3 ) page： 68 - 70   2022.10

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    Language：Japanese   Publishing type：Article, review, commentary, editorial, etc. (scientific journal)   Publisher：Japanese Society for Thermal Medicine  

    DOI： 10.3191/thermalmed.38.68

    CiNii Research

  3. 研究室紹介 Invited

    井藤　彰

    化学工学   Vol. 86 ( 8 ) page： 407 - 407   2022

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    Authorship：Lead author   Language：Japanese   Publishing type：Article, review, commentary, editorial, etc. (scientific journal)  

  4. 若手→中堅の生物化学工学者、学会について考える Invited

    井藤　彰

    化学工学会バイオ部会ニュースレター   ( 52 ) page： 1 - 2   2020.12

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    Authorship：Lead author,　Last author,　Corresponding author   Language：Japanese   Publishing type：Article, review, commentary, editorial, etc. (scientific journal)  

  5. 温熱誘導型遺伝子発現システムの開発と応用

    井藤彰

    放射線生物研究   Vol. 53 ( 2 ) page： 104‐115   2018.7

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    Language：Japanese  

    J-GLOBAL

  6. 筋芽細胞と繊維芽細胞との共培養による人工骨格筋組織の作製

    高橋克成, 井藤彰, 吉開太一, 池田一史, 河邉佳典, 上平正道

    化学工学会年会研究発表講演要旨集(CD-ROM)   Vol. 83rd   page： ROMBUNNO.PB224   2018.3

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    J-GLOBAL

  7. レトロトランスポゾンベクターの開発とCHO細胞への遺伝子導入

    高橋真美加, 河邉佳典, 村上舞, 吉田宗一郎, 井藤彰, 上平正道

    化学工学会年会研究発表講演要旨集(CD-ROM)   Vol. 83rd   page： ROMBUNNO.PB205   2018.3

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    Language：Japanese  

    J-GLOBAL

  8. ヒトiPS細胞由来骨格筋細胞分化培養プロセスの開発

    堀江正信, 井藤彰, 上平正道

    化学工学会年会研究発表講演要旨集(CD-ROM)   Vol. 83rd   page： ROMBUNNO.PB225   2018.3

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    J-GLOBAL

  9. 熱ショックによって高肝機能を誘導可能なヒトヘパトーマ細胞の作製

    北野裕之, 永江裕樹, 河邉佳典, 井藤彰, 上平正道

    化学工学会年会研究発表講演要旨集(CD-ROM)   Vol. 83rd   page： ROMBUNNO.PB230   2018.3

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    Language：Japanese  

    J-GLOBAL

  10. 遺伝子導入ニワトリによるTGF‐β1の卵白生産

    上平正道, 大坪嵩征, 小畑玲奈, 河邉佳典, 井藤彰

    日本農芸化学会大会講演要旨集(Web)   Vol. 2018   page： ROMBUNNO.2A24a05 (WEB ONLY)   2018.3

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    Language：Japanese  

    J-GLOBAL

  11. 逐次遺伝子組込みシステムにおけるミニサークルDNAによる遺伝子組込みの効率化

    河邉佳典, 汪雪, 井藤彰, 上平正道

    化学工学会秋季大会研究発表講演要旨集(CD-ROM)   Vol. 49th   page： ROMBUNNO.PB273   2017.9

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    J-GLOBAL

  12. hprt遺伝子座への抗体遺伝子の逐次組込みによる高生産CHO細胞株の構築

    羽田毅, 汪雪, 河邉佳典, 加藤梨沙, 井藤彰, 山名良正, 近藤雅子, 上平正道

    化学工学会秋季大会研究発表講演要旨集(CD-ROM)   Vol. 49th   page： ROMBUNNO.PB274   2017.9

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    J-GLOBAL

  13. 遺伝子改変フィーダー細胞を用いたiPS細胞の運動神経分化誘導

    吉岡貫太郎, 井藤彰, PAERWEN Paerhati, 河邉佳典, 上平正道

    日本生物工学会大会講演要旨集   Vol. 69th   page： 253   2017.8

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    J-GLOBAL

  14. レトロトランスポゾンを用いた遺伝子導入技術の開発

    村上舞, 河邉佳典, 吉田宗一郎, 井藤彰, 上平正道

    日本生物工学会大会講演要旨集   Vol. 69th   page： 158   2017.8

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    J-GLOBAL

  15. 低酸素応答型遺伝子発現システムを導入した細胞センサーの機能評価

    小野章彦, 井藤彰, 佐藤智詠, 山口雅紀, 鈴木大雅, 河邉佳典, 上平正道

    日本生物工学会大会講演要旨集   Vol. 69th   page： 314   2017.8

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    J-GLOBAL

  16. In vitro筋組織形成のための骨格筋細胞と繊維芽細胞との共培養

    高橋克成, 井藤彰, 今田隆介, 池田一史, 河邉佳典, 上平正道

    化学関連支部合同九州大会・外国人研究者交流国際シンポジウム講演予稿集   Vol. 54th   page： 30   2017.7

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    J-GLOBAL

  17. トランスジェニック鳥類作製のためのゲノム操作技術の開発

    前田大樹, 河邉佳典, 椎葉温, SHI Ming, 井藤彰, 上平正道

    化学関連支部合同九州大会・外国人研究者交流国際シンポジウム講演予稿集   Vol. 54th   page： 28   2017.7

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    J-GLOBAL

  18. 合成生物学的手法による高肝機能誘導型ヒトヘパトーマ細胞の作製

    北野裕之, 永江裕樹, 佐藤一輝, JANE Tonello, 河邉佳典, 井藤彰, 上平正道

    化学関連支部合同九州大会・外国人研究者交流国際シンポジウム講演予稿集   Vol. 54th   page： 28   2017.7

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    J-GLOBAL

  19. 生体アクチュエーターを用いた持続可能な動力供給システムの創出

    長森英二, 藤田英明, 森島圭祐, 秋山佳丈, 松崎典弥, 竹内昌治, 森本雄矢, 福田淳二, 古川英光, 蓮沼誠久, 井藤彰, 清水一憲, 櫻井英俊, 境慎司, 山田真澄, 山口哲志, 伊藤大知, 深田宗一朗, 今井祐記, 根建拓, 岸田綱郎, 藤田聡史, 冨田秀太, 加藤竜司, 備瀬竜馬, 上杉薫, 堀江正信

    豊田研究報告   ( 70 ) page： 243‐246   2017.5

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    J-GLOBAL

  20. Cre組込み型レンチウイルスベクターによるCHO細胞への特定ゲノム部位遺伝子導入法の開発

    羽田毅, 河邉佳典, 今西傑, 下村卓矢, 井藤彰, 上平正道

    化学工学会年会研究発表講演要旨集(CD-ROM)   Vol. 82nd   page： ROMBUNNO.PB232   2017.3

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    J-GLOBAL

  21. フェリチン遺伝子導入による細胞磁気標識技術の開発

    亀井一貴, 井藤彰, 小野章彦, 山口雅紀, 河邉佳典, 上平正道

    化学工学会年会研究発表講演要旨集(CD-ROM)   Vol. 82nd   page： ROMBUNNO.PB225   2017.3

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    J-GLOBAL

  22. バイオ人工肝臓システムのための高肝機能誘導型ヘパトーマ細胞株の作製と機能評価

    永江裕樹, 佐藤一輝, TONELLO Jane, 河邉佳典, 井藤彰, 上平正道

    化学工学会年会研究発表講演要旨集(CD-ROM)   Vol. 82nd   page： ROMBUNNO.PB213   2017.3

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    J-GLOBAL

  23. カドヘリン遺伝子導入フィーダー細胞を用いたiPS細胞の運動神経分化誘導

    吉岡貫太郎, PAERHATI Paerwen, 井藤彰, 河邉佳典, 上平正道

    化学工学会年会研究発表講演要旨集(CD-ROM)   Vol. 82nd   page： ROMBUNNO.PB219   2017.3

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    J-GLOBAL

  24. CRIS‐PITCh法を用いて作製したノックインCHO細胞の抗体生産評価

    河邉佳典, 小松眞也, 小松将大, 井藤彰, 佐久間哲史, 中村崇裕, 山本卓, 上平正道

    化学工学会年会研究発表講演要旨集(CD-ROM)   Vol. 82nd   page： ROMBUNNO.C120   2017.3

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    J-GLOBAL

  25. 遺伝子改変ヘパトーマ細胞の肝機能誘導における薬剤添加の影響

    上平正道, 佐藤一輝, TONELLO Jane, 河邉佳典, 井藤彰

    日本農芸化学会大会講演要旨集(Web)   Vol. 2017   page： ROMBUNNO.2J30p15 (WEB ONLY)   2017.3

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    Language：Japanese  

    J-GLOBAL

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To the head of MISC.▲

Presentations 54

  1. Expansion of Human Mesenchymal Stem Cells Using Collagen-modified Polyvinyl Alcohol Microcarriers International conference

    Masahiro Kaneko, Airi Sato, Satoru Ayano, Akio Fujita, Goro Kobayashi, Akira Ito

    JAACT2023  2023.12.1  JAACT

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    Event date： 2023.11 - 2023.12

    Language：English   Presentation type：Oral presentation (general)  

    Venue：Nagoya, Japan  

  2. 細胞外高分子物質の合成に向けた微生物誘導ラジカル重合法の開発

    杉浦健人、井藤彰、金子真大

    化学工学会秋季大会  2023.9.11  化学工学会

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    Event date： 2023.9

    Language：English   Presentation type：Poster presentation  

    Venue：福岡市  

  3. がんの温熱療法のための生体模倣リン脂質ポリマー被覆磁性ナノ粒子の開発

    雪下奏斗、金子真大、堤内要、井藤彰

    化学工学会秋季大会  2023.9.11  化学工学会

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    Event date： 2023.9

    Language：English   Presentation type：Poster presentation  

    Venue：福岡市  

  4. 磁気温熱療法における抗腫瘍免疫を強化するall-in-one磁性ナノ粒子の開発

    西川安美, 齋藤凛太郎, 金子真大, 井藤彰

    化学工学会秋季大会  2023.9.11  化学工学会

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    Event date： 2023.9

    Language：English   Presentation type：Poster presentation  

    Venue：福岡市  

  5. ポリビニルアルコール製マイクロキャリアを用いたヒト間葉系幹細胞の増幅

    佐藤愛梨、金子真大、綾野賢、藤田明士、小林悟朗、井藤彰

    化学工学会秋季大会  2023.9.11  化学工学会

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    Event date： 2023.9

    Language：English   Presentation type：Poster presentation  

    Venue：福岡市  

  6. 肝臓の凍結保存を指向した凍結保護液の開発

    若林大誠、金子真大、井藤彰

    化学工学会秋季大会  2023.9.11  化学工学会

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    Event date： 2023.9

    Language：English   Presentation type：Poster presentation  

    Venue：福岡市  

  7. バイオ人工膵臓のためのリン脂質ポリマー修飾アルギン酸ゲルの開発

    二ツ橋龍、金子真大、井藤彰

    日本生物工学会  2023.9.3  日本生物工学会

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    Event date： 2023.9

    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：名古屋市  

  8. がん治療のためのフェロセン含有リン脂質ポリマーの分子設計

    小河優玖, 金子真大, 井藤彰

    日本生物工学会  2023.9.5  日本生物工学会

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    Event date： 2023.9

    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：名古屋市  

  9. 血中投与によるがんの診断と治療を指向した リン脂質ポリマー被覆磁性ナノ粒子の開発

    大野孝紘、金子真大、井藤彰

    日本生物工学会  2023.9.5  日本生物工学会

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    Event date： 2023.9

    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：名古屋市  

  10. 磁性ナノ粒子を用いた医療技術の開発

    井藤　彰

    名古屋大学オープンキャンパス  2023.8.7  名古屋大学

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    Event date： 2023.8

    Language：Japanese   Presentation type：Public lecture, seminar, tutorial, course, or other speech  

    Venue：名古屋大学  

  11. 磁性ナノ粒子の血中投与を指向したリン脂質ポリマー被覆磁性ナノ粒子の開発

    金子真大、大野孝紘、井藤彰

    中部ハイパーサーミア研究学術集会  2023.7.22  中部ハイパーサーミア研究会

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    Event date： 2023.7

    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：名古屋市  

  12. 磁性ナノ粒子を用いた医療技術の開発

    井藤　彰

    名古屋大学iMIｘ信州大学RISM合同セミナー  2023.3.17  信州大学先端材料研究所

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    Event date： 2023.3

    Language：Japanese   Presentation type：Public lecture, seminar, tutorial, course, or other speech  

    Venue：名古屋大学  

  13. ナノ・ヒーティングによる生体組織凍結保存技術の創出

    井藤　彰

    さきがけ「熱制御」公開シンポジウム  2023.3.7  JSTさきがけ

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    Event date： 2023.3

    Language：Japanese   Presentation type：Symposium, workshop panel (nominated)  

    Venue：AP市ヶ谷  

  14. 機能性磁性ナノ粒子を用いた医療技術の開発 Invited

    井藤　彰

    第３５回ＣＥＳ２１講演会「医薬分野で活躍する化学工学」  2023.1.27  化学工学会関西支部

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    Event date： 2023.1

    Language：Japanese   Presentation type：Public lecture, seminar, tutorial, course, or other speech  

    Venue：大阪市  

  15. 機能性磁性ナノ粒子を用いたガン温熱療法の開発 Invited

    井藤　彰

    第31回日本磁気科学会2022 分科会研究会-高分子・材料プロセス分科会-  2022.12.12 

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    Event date： 2022.12

    Language：Japanese   Presentation type：Oral presentation (invited, special)  

    Venue：日本磁気科学会  

  16. My memmory of JBB Invited International conference

    Akira Ito

    2022.10.20 

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    Event date： 2022.10

    Language：English   Presentation type：Oral presentation (invited, special)  

    Country：Japan  

  17. フェロセン含有リン脂質ポリマーによるがん細胞の免疫原性細胞死誘導

    山口 明生, 金子 真大, 井藤 彰

    創立100周年記念日本生物工学会大会  2022.10.18  日本生物工学会

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    Event date： 2022.10

    Language：Japanese   Presentation type：Poster presentation  

    Venue：大阪市  

  18. 高機能性ビニルアルコール系重合体を用いたバイオ人工膵臓の開発

    森口 浩聡, 金子 真大, 井藤 彰

    創立100周年記念日本生物工学会大会  2022.10.19  日本生物工学会

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    Event date： 2022.10

    Language：Japanese   Presentation type：Poster presentation  

    Venue：大阪市  

  19. ミトコンドリア標的型磁性ナノ粒子によるがん温熱療法

    山﨑 裕永, 金子 真大, 井藤 彰

    創立100周年記念日本生物工学会大会  2022.10.19  日本生物工学会

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    Event date： 2022.10

    Language：Japanese   Presentation type：Poster presentation  

    Venue：大阪市  

  20. 磁性ナノ粒子を用いたナノウォーミングによる膵島の凍結保存

    若林 大誠, 中井 友規, 金子 真大, 井藤 彰

    化学工学会第53回秋季大会  2022.9.14  化学工学会

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    Event date： 2022.9

    Language：Japanese   Presentation type：Poster presentation  

    Venue：長野  

  21. 腫瘍微小環境をターゲットとした温熱免疫療法

    西川 安美, 鈴木 悠太郎, 金子 真大, 井藤 彰

    化学工学会第53回秋季大会  2022.9.14  化学工学会

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    Event date： 2022.9

    Language：Japanese   Presentation type：Poster presentation  

    Venue：長野  

  22. 人工細胞外マトリックスの合成を指向した微生物誘導型ラジカル重合法の開発

    杉浦 健人, 井藤 彰, 金子 真大

    化学工学会第53回秋季大会  2022.9.14  化学工学会

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    Event date： 2022.9

    Language：Japanese   Presentation type：Poster presentation  

    Venue：長野  

  23. Hyperthermia using functional magnetic nanoparticles Invited

    2021.9.1 

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    Event date： 2021.8 - 2021.9

    Presentation type：Symposium, workshop panel (nominated)  

  24. 磁性ナノテクノロジーによるヒトiPS細胞の凍結保存

    井藤　彰

    化学工学会第51回秋季大会  2020.9.26  化学工学会

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    Event date： 2020.9

    Language：Japanese   Presentation type：Symposium, workshop panel (public)  

  25. 磁性ナノ粒子を用いた生体組織の凍結保存

    中井友規、金子真大、堀江正信、井藤　彰

    化学工学会第51回秋季大会  2020.9.25  化学工学会

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    Event date： 2020.9

    Language：Japanese   Presentation type：Symposium, workshop panel (public)  

  26. 磁性ナノ粒子を用いたハイパーサーミア －基礎研究の新展開 Invited

    井藤　彰、金子真大

    日本ハイパーサーミア学会第37回大会  2020.9.12  日本ハイパーサーミア学会第37回大会

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    Event date： 2020.9

    Language：Japanese   Presentation type：Symposium, workshop panel (nominated)  

  27. Medical application of functional magnetic nanoparticles Invited International conference

    Akira Ito

    International confrence on material and systems for sustainability 2019 

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    Event date： 2019.11

    Language：English   Presentation type：Oral presentation (general)  

    Venue：Nagoya   Country：Japan  

  28. 磁力を用いた三次元筋組織構築とin vitro評価系の開発

    井藤　彰、堀江正信、堀田秋津、櫻井英俊

    骨格筋スマート社会実現コンソーシアム講演会 

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    Event date： 2019.9

    Language：Japanese   Presentation type：Symposium, workshop panel (nominated)  

    Venue：大阪市   Country：Japan  

  29. 機能性磁性ナノ粒子の開発と医療分野への応用 Invited

    井藤　彰

    日本生物工学会中部支部例会 

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    Event date： 2019.8

    Language：Japanese   Presentation type：Oral presentation (invited, special)  

    Venue：岐阜市   Country：Japan  

  30. 機能性磁性ナノ粒子を用いたハイパーサーミア Invited

    井藤　彰

    東海ハイパーサーミア研究会 

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    Event date： 2019.7

    Language：Japanese   Presentation type：Oral presentation (invited, special)  

    Venue：名古屋市   Country：Japan  

  31. 機能性磁性ナノ粒子の開発と医療技術への応用に関する生物工学的研究 Invited

    井藤　彰

    日本生物工学会 

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    Event date： 2018.9

    Language：Japanese   Presentation type：Oral presentation (invited, special)  

    Country：Japan  

  32. 機能性磁性ナノ粒子を用いた生体加温技術 Invited

    井藤　彰

    Biothermology 2017 

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    Event date： 2017.12

    Language：Japanese   Presentation type：Oral presentation (invited, special)  

    Country：Japan  

  33. 日本学術会議若手アカデミー -設置の背景と取り組み- Invited

    井藤　彰

    日本体育学会 

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    Event date： 2017.9

    Language：Japanese   Presentation type：Oral presentation (invited, special)  

    Country：Japan  

  34. Hyperthermia using functional magnetite nanoparticles Invited

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    Event date： 2016.11

    Language：English   Presentation type：Oral presentation (invited, special)  

    Country：Japan  

  35. 磁性ナノ粒子を用いた磁場誘導型遺伝子発現法の開発 Invited

    井藤　彰

    日本ハイパーサーミア学会 

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    Event date： 2016.9

    Language：Japanese   Presentation type：Oral presentation (invited, special)  

    Country：Japan  

  36. 磁性ナノ粒子を用いたティッシュエンジニアリング技術の開発

    井藤　彰

    日本機械学会 第２８回バイオエンジニアリング講演会 

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    Event date： 2016.1

    Language：Japanese   Presentation type：Oral presentation (invited, special)  

    Country：Japan  

  37. 磁気細胞操作によるティッシュエンジニアリング技術の開発 Invited

    井藤　彰

    化学とマイクロ・ナノシステム学会 

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    Event date： 2015.11

    Language：Japanese   Presentation type：Oral presentation (invited, special)  

    Country：Japan  

  38. 機能性磁性ナノ粒子を用いた医療技術の開発 Invited

    井藤　彰

    日本泌尿器学会  

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    Event date： 2014.10

    Language：Japanese   Presentation type：Oral presentation (invited, special)  

    Country：Japan  

  39. Medical Application of Magnetite Nanoparticles Invited

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    Event date： 2014.3

    Language：English   Presentation type：Oral presentation (invited, special)  

    Country：Japan  

  40. 機能性磁性ナノ粒子を用いた磁場誘導型温熱遺伝子治療システムの開発 Invited

    井藤　彰, 山口雅紀, 河邉 佳典, 上平 正道

    日本ハイパーサーミア学会 

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    Event date： 2013.8

    Language：Japanese   Presentation type：Oral presentation (invited, special)  

    Country：Japan  

  41. ナノテクノロジーが創る新しい医療 Invited

    井藤　彰

    2013年度 読売テクノ・フォーラム ゴールド・メダル賞受賞記念講演会 

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    Event date： 2013.7

    Language：Japanese   Presentation type：Oral presentation (invited, special)  

    Country：Japan  

  42. ナノテクノロジーが創る新しい医療 Invited

    井藤　彰

    2013年度 読売テクノ・フォーラム ゴールド・メダル賞受賞記念講演会 

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    Event date： 2013.5

    Language：Japanese   Presentation type：Oral presentation (invited, special)  

    Country：Japan  

  43. Medical Application of Functional Magnetite Nanoparticles Invited International conference

    井藤　彰

    AUMS 

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    Event date： 2012.10

    Language：English   Presentation type：Oral presentation (invited, special)  

    Country：Japan  

  44. Hyperthermia using functional magnetite nanoparticles Invited International conference

    井藤　彰

    BioEM2009  

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    Event date： 2009.6

    Language：English   Presentation type：Oral presentation (keynote)  

    Country：Switzerland  

  45. 機能性磁性ナノ粒子を用いたティッシュエンジニアリング技術の開発 Invited

    井藤　彰

    化学工学会 

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    Event date： 2008.3

    Language：Japanese   Presentation type：Oral presentation (invited, special)  

    Country：Japan  

  46. 機能性磁性ナノ粒子を用いた三次元組織構築技術 Invited

    井藤　彰, 本多裕之, 上平正道

    日本薬学会 

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    Event date： 2007.3

    Language：Japanese   Presentation type：Oral presentation (invited, special)  

    Country：Japan  

  47. 磁性ナノ粒子を用いた組織再生 Invited

    井藤　彰, 本多裕之, 上平正道

    日本バイオマテリアル学会 

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    Event date： 2006.11

    Language：Japanese   Presentation type：Oral presentation (invited, special)  

    Country：Japan  

  48. 磁力を用いた三次元筋組織構築とin vitro評価系の開発 International conference

    井藤 彰, 堀江正信, 堀田秋津, 櫻井英俊

    骨格筋スマート社会実現コンソーシアム講演会  2019.9.19 

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    Language：Japanese   Presentation type：Symposium, workshop panel (nominated)  

    Venue：大阪市  

  49. 機能性磁性ナノ粒子を用いた生体加温技術 Invited International conference

    井藤 彰

    Biothermology 2017  2017.12.25 

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    Language：Japanese   Presentation type：Oral presentation (invited, special)  

  50. 機能性磁性ナノ粒子を用いたハイパーサーミア Invited International conference

    井藤 彰

    東海ハイパーサーミア研究会  2019.7.20  東海ハイパーサーミア研究会

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    Language：Japanese   Presentation type：Oral presentation (invited, special)  

    Venue：名古屋市  

  51. 機能性磁性ナノ粒子の開発と医療技術への応用に関する生物工学的研究 Invited International conference

    井藤 彰

    日本生物工学会  2018.9.6 

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    Language：Japanese   Presentation type：Oral presentation (invited, special)  

  52. 機能性磁性ナノ粒子の開発と医療分野への応用 Invited International conference

    井藤 彰

    日本生物工学会中部支部例会  2019.8.6  日本生物工学会中部支部

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    Language：Japanese   Presentation type：Oral presentation (invited, special)  

    Venue：岐阜市  

  53. 日本学術会議若手アカデミー -設置の背景と取り組み- Invited International conference

    井藤 彰

    日本体育学会  2017.9.8 

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    Language：Japanese   Presentation type：Oral presentation (invited, special)  

  54. Medical application of functional magnetic nanoparticles Invited

    Akira Ito

    International confrence on material and systems for sustainability 2019  2019.11.3 

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    Language：English   Presentation type：Oral presentation (general)  

    Venue：Nagoya  

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To the head of Presentations.▲

Works 2

  1. 報告 若手研究者ネットワークの継続的運用に向けて

    2014.9

  2. 提言 若手アカデミー設置について

    2011.9

To the head of Works.▲

Research Project for Joint Research, Competitive Funding, etc. 13

  1. 免疫隔離デバイスを用いた糖尿病治療に関する研究

    2023.1 - 2023.12

    企業からの受託研究  

    井藤　彰

    　 More details

    Authorship：Principal investigator  Grant type：Collaborative (industry/university)

    Grant amount：\2000000 （ Direct Cost: \1539000 、 Indirect Cost：\461000 ）

  2. 癌の診断と治療を同時に可能にする磁性ナノテクノロジーの創成

    2022.11 - 2024.3

    財団からの研究助成 

    井藤　彰

    　 More details

    Authorship：Principal investigator 

    Grant amount：\2000000 （ Direct Cost: \2000000 ）

  3. 機能性磁性ナノ粒子を用いた磁場誘導型がん温熱療法の開発

    2022.10 - 2023.11

    財団からの研究助成 

    井藤　彰

    　 More details

    Authorship：Principal investigator 

    Grant amount：\3000000 （ Direct Cost: \3000000 ）

  4. 癌の診断と治療を同時に可能にする磁性ナノテクノロジーの創成

    2022.9 - 2024.9

    財団からの研究助成 

    井藤　彰

    　 More details

    Authorship：Principal investigator 

    Grant amount：\3000000 （ Direct Cost: \3000000 ）

  5. 免疫隔離デバイスを用いた糖尿病治療に関する研究

    2022.1 - 2022.12

    企業からの受託研究  

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    Authorship：Principal investigator  Grant type：Collaborative (industry/university)

    Grant amount：\3000000

  6. 30周年記念特別研究助成金

    2021.4 - 2024.3

    財団からの研究助成  磁場誘導加温による生体組織の凍結保存技術の開発

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    Authorship：Principal investigator 

    Grant amount：\5000000 （ Direct Cost: \5000000 ）

  7. 細胞培養担体の機能性評価

    2019.12

    企業からの受託研究 

  8. 高機能性ビニルアルコール系重合体を用いた革新的バイオ人工膵臓の開発

    2019.7 - 2022.3

    AMED 医療分野研究成果展開事業（先端計測分析技術・機器開発プログラム） 

    井藤　彰

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    Grant type：Competitive

    本研究ではヒトiPS細胞から分化誘導した膵島細胞を搭載したエチレン－ビニルアルコール共重合体（EVOH）からなるデバイスを用いた「バイオ人工膵臓」を開発し、「EVOHデバイス」を用いることで重篤な副作用や医療費負担を伴う免疫抑制剤を使用することなく、長期にわたる移植細胞の機能性を保持し、ヒトiPS細胞を用いた細胞移植治療の実現を目指す。

  9. ナノ・ヒーティングによる生体組織凍結保存技術の創出

    2019.5

    企業からの受託研究  

    　 More details

    Authorship：Principal investigator 

  10. 培養を必要としない細胞成型技術のための機器開発

    2019

    企業からの受託研究  

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    Authorship：Coinvestigator(s) 

  11. ナノ・ヒーティングによる生体組織凍結保存技術の創出

    2017.10 - 2021.3

    さきがけ 

    井藤　彰

    　 More details

    Grant type：Competitive

    臓器や組織を冷凍保存できれば、移植ドナー不足を解消できます。また、再生医療において、移植用組織や細胞の凍結保存技術は実用化のために必須です。本研究では、磁性ナノ粒子の交流磁場中における発熱のスペクトル学的理解と制御技術の開発により、従来困難であった生体組織の凍結保存技術を確立することを目的とします。本研究の成果は、ナノテクノロジーを駆使した新しい技術として、国民の健康に寄与すると期待されます。

  12. 筋組織形成促進および筋萎縮抑制効果を有する素材の探索

    2014

    企業からの受託研究 

    　 More details

    独自に開発したティッシュエンジニアリング技術で作製した三次元筋組織を用いて、収縮力を指標にしたサルコペニア抑制素材の検索を行った。

  13. 三次元組織構築

    2007 - 2008

    企業からの受託研究 

    　 More details

    磁性ナノ粒子を用いた新規三次元筋組織構築法の開発を行った。

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To the head of Research Project for Joint Research, Competitive Funding, etc..▲

KAKENHI (Grants-in-Aid for Scientific Research) 23

  1. Development of functional magnetic nanoparticle for cancer theranostics

    Grant number：23H01772  2023.4 - 2026.3

    Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

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    Authorship：Principal investigator  Grant type：Competitive

    Grant amount：\18720000 （ Direct Cost: \14400000 、 Indirect Cost：\4320000 ）

  2. Development of functional magnetic nanoparticle for cancer theranostics

    Grant number：23K26465  2023.4 - 2026.3

    Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

    　 More details

    Authorship：Principal investigator 

    Grant amount：\18720000 （ Direct Cost: \14400000 、 Indirect Cost：\4320000 ）

  3. メカノセンサー標的型磁性ナノ粒子の創製とヒトiPS細胞精密挙動制御技術の開発

    Grant number：2721K04787  2021.4 - 2024.3

    科研費  基盤研究C

    井藤　彰

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    Authorship：Coinvestigator(s)  Grant type：Competitive

    Grant amount：\300000 （ Direct Cost: \300000 、 Indirect Cost：\90000 ）

  4. メカノセンサー標的型磁性ナノ粒子の創製とヒトiPS細胞精密挙動制御技術の開発

    Grant number：21K04787  2021.4 - 2024.3

    日本学術振興会  科学研究費助成事業  基盤研究(C)

    堀江 正信, 井藤 彰

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    Authorship：Coinvestigator(s) 

    申請者らはこれまで工学的な新しいヒトiPS細胞培養技術として、外部物理刺激負荷のみで挙動を自在に制御する技術開発を行ってきた。しかし、細胞外部環境および物理刺激の受容が均一でなく、細胞外物理刺激のみでは挙動制御が困難であった。本研究では、生体適合性の高い酸化鉄マグネタイト (Fe3O4)の磁性ナノ粒子を用いた薬物送達システム（Drug delivery system, DDS）を駆使して細胞内DDSを構築し、細胞骨格や核といった細胞内物理刺激受容体（メカノセンサー）を標的とした物理刺激を細胞内部から直接行い、挙動評価を行うことでメカノセンサーとヒトiPS細胞挙動の関係性を探る。
    本年度はメカノセンサー標的型粒子の設計と開発、および物理刺激がヒトiPS細胞に与える影響評価を主として行なった。具体的には直径約10 nmのマグネタイ ト粒子の表面をアミノシランコートしPEG鎖を導入する。さらにミトコンドリア指向性化合物TPPおよび核移行シグナルペプチドを提示させることによって、それぞれミトコンドリアおよび細胞核への送達を目指した。構築したマグネタイト粒子を正電荷脂質膜で包埋することによって、負の電荷を持つ細胞表面に自身で結合し取り込まれる。リポソームの主成分であるDOPEが膜融合することによってマグネタイト粒子はエンドソーム脱出するものと考えた。
    当該粒子を構築後テストとして、まずマウスKRAS変異大腸がんCT26細胞(BALB/c)に取り込ませ、集積や細胞影響を評価した。コントロールとしてミトコンドリアターゲッティング部位（TPP）有無の2種類を構築し、評価を行った。その結果、細胞への取り込み量、交流磁場照射による温度上昇はTPPの有無に関わらずに同等であった。一方で、細胞生存率に関してはTPP無しの場合、70%程度であったのに対して、TPPありの場合、40%程度まで低下しており、効果的に細胞を殺傷していることが確認できた。さらに、当該粒子を取り込ませた上記細胞を電子顕微鏡によって観察したところ、TPP提示粒子の方が優位にミトコンドリアに集積していることが確認できた。
    ミトコンドリア標的型磁性粒子の構築は完了し、ガン細胞をモデルとして標的性や機能確認を終えることができている。それらの結果を論文としてまとめることができている。
    構築したミトコンドリア標的型磁性ナノ粒子をヒトiPS細胞に取りませ、上記と同様に取り込み量及び交流磁場による発熱、さらに電子顕微鏡を用いたミトコンドリアへの集積を評価する予定である。それらの評価と同時に、ネオジム磁石を用いた細胞内の局所物理刺激などの負荷と細胞応答評価を行っていく。

  5. Development of nanoheaters targeting organellas for cancer hyperthermia

    Grant number：20H02538  2020.4 - 2023.3

    Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

    Ito Akira

    　 More details

    Authorship：Principal investigator  Grant type：Competitive

    Grant amount：\17680000 （ Direct Cost: \13600000 、 Indirect Cost：\4080000 ）

    Advances in nanotechnology have produced nano-sized magnetic particles (nanoheaters) that generate heat in an alternating magnetic field. As a new engineering-based cancer treatment technology, we delivered magnetic nanoparticles of highly biocompatible iron oxide (magnetite) to tumors using a drug delivery system (DDS), and externally irradiated magnetic nanoparticles with an alternating magnetic field. In this study, we constructed a further developed intracellular DDS and performed mitochondria-targeted heating, which resulted in complete tumor regression in 5 out of 6 mice. These results suggest that organelle-targeted nanoheaters will lead to the development of potent cancer hyperthermia.

  6. 高機能性ビニルアルコール系重合体を用いた革新的バイオ人工膵臓の開発

    2019.7 - 2022.3

    AMED  AMED 医療分野研究成果展開事業（先端計測分析技術・機器開発プログラム） 

    井藤 彰, 株式会社クラレ, 国立国際医療研究センター

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    Authorship：Principal investigator  Grant type：Competitive

    Grant amount：\36800000 （ Direct Cost: \16000000 、 Indirect Cost：\20800000 ）

    本研究ではヒトiPS細胞から分化誘導した膵島細胞を搭載したエチレン－ビニルアルコール共重合体（EVOH）からなるデバイスを用いた「バイオ人工膵臓」を開発し、「EVOHデバイス」を用いることで重篤な副作用や医療費負担を伴う免疫抑制剤を使用することなく、長期にわたる移植細胞の機能性を保持し、ヒトiPS細胞を用いた細胞移植治療の実現を目指す。

  7. Cancer heat immunotherapy using magnetic nanoparticles

    Grant number：19K22086  2019.6 - 2022.3

    Grants-in-Aid for Scientific Research  Grant-in-Aid for Challenging Research (Exploratory)

    Ito Akira

    　 More details

    Authorship：Principal investigator  Grant type：Competitive

    Grant amount：\6500000 （ Direct Cost: \5000000 、 Indirect Cost：\1500000 ）

    Immune checkpoint inhibitors induce cytotoxic T lymphocytes but they can kill a limited number of cancer cells, which is difficult to treat solid tumors. We have been developing hyperthermia that destroys cancer by heat by utilizing the property that magnetic nanoparticles generate heat under an alternating magnetic field. Hyperthermia using magnetic nanoparticles 1) can produce / release a large amount of tumor antigen recognized by the immune system in the body, 2) tumor tissue can be destroyed by hyperthermia, and metastatic cancer can be eliminated by immunity, which enables ideal cancer treatment that strongly complements the weak points of immune checkpoint inhibitors. In this study, we have developed a novel Heat Immunotherapy that strongly amplifies the effects of immune checkpoint inhibitors by using magnetic nanotechnology.

  8. ナノ・ヒーティングによる生体組織凍結保存技術の創出

    2017.10 - 2021.3

    JST  さきがけ 

    井藤 彰

    　 More details

    Authorship：Principal investigator  Grant type：Competitive

    臓器や組織を冷凍保存できれば、移植ドナー不足を解消できます。また、再生医療において、移植用組織や細胞の凍結保存技術は実用化のために必須です。本研究では、磁性ナノ粒子の交流磁場中における発熱のスペクトル学的理解と制御技術の開発により、従来困難であった生体組織の凍結保存技術を確立することを目的とします。本研究の成果は、ナノテクノロジーを駆使した新しい技術として、国民の健康に寄与すると期待されます。

  9. Skeletal muscle tissue engineering using human iPS cells

    Grant number：17H03469  2017.4 - 2020.3

    Ito Akira

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    Authorship：Principal investigator  Grant type：Competitive

    Grant amount：\17550000 （ Direct Cost: \13500000 、 Indirect Cost：\4050000 ）

    The most important function of skeletal muscle is to generate force by　contracting. However, by inducing differentiation of human iPS cells, a method of producing mature three-dimensional muscle tissue exhibiting contraction force has not been established. In this study, we developed a process for constructing "contracting" three-dimensional muscle tissue from human iPS cells based on the advanced medical engineering technology "Magnetic Nanotechnology". The three-dimensional muscle tissues developed in this study are considered to be useful for regenerative medicine of skeletal muscle, elucidation of muscle disease mechanisms, and drug screening for muscle dystrophy and age-related muscle dystrophy (sarcopenia).

  10. ヒトiPS細胞を用いた骨格筋ティッシュエンジニアリング技術の開発

    2017.4 - 2020.3

    日本学術振興会  科学研究費助成事業  基盤研究(B)

    井藤 彰, 堀江正信

    　 More details

    Authorship：Principal investigator  Grant type：Competitive

    再生医療の研究において、iPS細胞から生体内の機能的な細胞への分化誘導法の開発が行われており、骨格筋においても筋転写因子の一つであるMyoD遺伝子をiPS細胞に導入することで、筋芽細胞へ分化することが報告されている。さらに、筋疾患患者由来の細胞から筋疾患iPS細胞を作製し、筋疾患に関する遺伝子を高効率かつ高精度に修復できることも示されている。しかしながら、筋肉の最も重要な機能である収縮を指標とした評価はなされていない。本研究では、iPS細胞から「動く」筋組織を作製して、収縮力を指標とした筋機能評価という新しい評価法を提案する。我々オリジナルの磁性ナノテクノロジーを基盤とした三次元組織作製法（Mag-TE法）により、MEMS技術により作製したPDMSの微細デバイスを用いて、iPS細胞から三次元筋組織組織を作製したところ、電気刺激で収縮して力を発生する組織を構築することに成功した。この成果は、骨格筋組織再生や薬剤スクリーニングへの応用に向けた人工筋組織の作製にとって有用な知見となることが期待できる。

  11. Research on innovative cell transplantation device using immunoisolation membrane

    Grant number：16K01362  2016.4 - 2019.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    Ando Yusuke, Nakaji Shuhei, Kobayashi Goro

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    Authorship：Coinvestigator(s)  Grant type：Competitive

    The formation of a semipermeable membrane made of ethylene-vinyl alcohol copolymer to be used for a cell transplantation device was investigated, and it became possible to stably produce membranes with different pore sizes. The permeability of glucose and various proteins was examined using membranes with different pore sizes, and it was clarified that separation of proteins is possible by pore size. We found a membrane impermeable to IgG, indicating the possibility of protecting antibody permeation. Furthermore, it was confirmed that cells did not permeate through any of the membranes. On the other hand, for the evaluation of cells, preparation of pancreatic β cells from human iPS cells was performed, and evaluation was performed to confirm that the prepared pancreatic β cells secrete insulin depending on high concentration of glucose. The cells were enclosed in a device and transplantation to animals was also attempted.

  12. 磁性ナノテクノロジーによる細胞内局所加温技術の創出

    2016.4 - 2018.3

    科学研究費補助金 

    井藤　彰

    　 More details

    Authorship：Principal investigator 


    磁性ナノ粒子は磁石に引き寄せられる性質のみならず、MRIにおける造影効果や、交流磁場中で発熱する性質をもつ。本研究では、酸化鉄の10 nmのマグネタイト表面を正電荷脂質膜で修飾することでマグネタイトカチオニックリポソームを作製し、単一細胞が加温可能な技術を開発した。方法論として、合成生物学的アプローチを導入して、レポーター遺伝子の発現を指標に細胞の熱ストレスを解析するシステムを構築した。HSP70B’プロモーターとTet-Offシステムの融合により構築した熱誘導型人工プロモーターは、熱ショックがかかるとポジティブフィードバックシステムにより、EGFPが高発現し続けることで、温熱ストレスを被った履歴のある細胞が可視的に判別できる。細胞に機能性磁性ナノ粒子を取り込ませて、交流磁場を照射すると、培地の温度上昇は見られないが、目的遺伝子が発現した。このことは、細胞内局所における磁性ナノ粒子の発熱によって、HSP70B’プロモーターが駆動したと考えられる。さらに、磁性ナノ粒子を合成生物学的アプローチで細胞内に生産させる方法を開発した。鉄貯蔵タンパク質であるフェリチンの遺伝子をHeLa細胞に導入して磁性ナノ粒子を細胞内で形成させることによって、遺伝子的に細胞を磁気標識することに成功した。フェリチン遺伝子の発現により磁気標識された細胞は、磁気分離が可能となり、また、MRIの造影効果が得られた。さらに、熱誘導型人工プロモーターを導入したHepG2細胞にフェリチン遺伝子を導入して磁場を照射すると、レポーター遺伝子であるEGFPが発現することから、フェリチン導入細胞が交流磁場で発熱することを見いだした。今後、EGFPを治療遺伝子などの目的遺伝子に交換することで、磁場誘導型の遺伝子治療といった医療への応用が可能であると考えられる。

  13. 磁性ナノテクノロジーによる細胞内局所加温技術の創出

    2016.4 - 2018.3

    科学研究費助成事業  新学術領域研究（研究領域提案型）

    井藤 彰

    　 More details

    Authorship：Principal investigator  Grant type：Competitive

    磁性ナノ粒子は磁石に引き寄せられる性質のみならず、MRIにおける造影効果や、交流磁場中で発熱する性質をもつ。本研究では、酸化鉄の10 nmのマグネタイト表面を正電荷脂質膜で修飾することでマグネタイトカチオニックリポソームを作製し、単一細胞が加温可能な技術を開発した。方法論として、合成生物学的アプローチを導入して、レポーター遺伝子の発現を指標に細胞の熱ストレスを解析するシステムを構築した。HSP70B’プロモーターとTet-Offシステムの融合により構築した熱誘導型人工プロモーターは、熱ショックがかかるとポジティブフィードバックシステムにより、EGFPが高発現し続けることで、温熱ストレスを被った履歴のある細胞が可視的に判別できる。細胞に機能性磁性ナノ粒子を取り込ませて、交流磁場を照射すると、培地の温度上昇は見られないが、目的遺伝子が発現した。このことは、細胞内局所における磁性ナノ粒子の発熱によって、HSP70B’プロモーターが駆動したと考えられる。さらに、磁性ナノ粒子を合成生物学的アプローチで細胞内に生産させる方法を開発した。鉄貯蔵タンパク質であるフェリチンの遺伝子をHeLa細胞に導入して磁性ナノ粒子を細胞内で形成させることによって、遺伝子的に細胞を磁気標識することに成功した。フェリチン遺伝子の発現により磁気標識された細胞は、磁気分離が可能となり、また、MRIの造影効果が得られた。さらに、熱誘導型人工プロモーターを導入したHepG2細胞にフェリチン遺伝子を導入して磁場を照射すると、レポーター遺伝子であるEGFPが発現することから、フェリチン導入細胞が交流磁場で発熱することを見いだした。今後、EGFPを治療遺伝子などの目的遺伝子に交換することで、磁場誘導型の遺伝子治療といった医療への応用が可能であると考えられる。

  14. 磁性ナノテクノロジーによる細胞内局所加温技術の創出

    Grant number：16H01393  2016.4 - 2018.3

    新学術領域研究(研究領域提案型)

    井藤 彰

    　 More details

    Authorship：Principal investigator  Grant type：Competitive

    Grant amount：\8970000 （ Direct Cost: \6900000 、 Indirect Cost：\2070000 ）

    磁性ナノ粒子は磁石に引き寄せられる性質のみならず、MRIにおける造影効果や、交流磁場中で発熱する性質をもつ。本研究では、酸化鉄の10 nmのマグネタイト表面を正電荷脂質膜で修飾することでマグネタイトカチオニックリポソームを作製し、単一細胞が加温可能な技術を開発した。方法論として、合成生物学的アプローチを導入して、レポーター遺伝子の発現を指標に細胞の熱ストレスを解析するシステムを構築した。HSP70B’プロモーターとTet-Offシステムの融合により構築した熱誘導型人工プロモーターは、熱ショックがかかるとポジティブフィードバックシステムにより、EGFPが高発現し続けることで、温熱ストレスを被った履歴のある細胞が可視的に判別できる。細胞に機能性磁性ナノ粒子を取り込ませて、交流磁場を照射すると、培地の温度上昇は見られないが、目的遺伝子が発現した。このことは、細胞内局所における磁性ナノ粒子の発熱によって、HSP70B’プロモーターが駆動したと考えられる。さらに、磁性ナノ粒子を合成生物学的アプローチで細胞内に生産させる方法を開発した。鉄貯蔵タンパク質であるフェリチンの遺伝子をHeLa細胞に導入して磁性ナノ粒子を細胞内で形成させることによって、遺伝子的に細胞を磁気標識することに成功した。フェリチン遺伝子の発現により磁気標識された細胞は、磁気分離が可能となり、また、MRIの造影効果が得られた。さらに、熱誘導型人工プロモーターを導入したHepG2細胞にフェリチン遺伝子を導入して磁場を照射すると、レポーター遺伝子であるEGFPが発現することから、フェリチン導入細胞が交流磁場で発熱することを見いだした。今後、EGFPを治療遺伝子などの目的遺伝子に交換することで、磁場誘導型の遺伝子治療といった医療への応用が可能であると考えられる。
    29年度が最終年度であるため、記入しない。
    29年度が最終年度であるため、記入しない。

  15. 磁性ナノテクノロジーによる骨格筋再生医療の技術基盤の創製

    2014.4 - 2017.3

    科学研究費補助金  基盤研究(B)

    井藤　彰

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    Authorship：Principal investigator 

    本研究では、骨格筋再生医療における「再生治療」と「再生研究」に有用な新規技術基盤の開発を行った。特に、我々がこれまで開発してきた先進医工学技術「磁性ナノテクノロジー」を効果的に利用し、独創的な方法論で研究展開をはかった。骨格筋は組織を形成して初めて本来の機能を発揮する。磁力を用いた組織工学技術により、電気刺激によって「動く」筋組織を作製することで、移植組織の「質」を評価する「再生治療」の評価基盤の確立を行った。また、再生治療研究として、ヒトiPS細胞の筋分化法の検討を行った。さらに、微細加工技術を組み合わせることで筋組織アレイを開発し、新規薬剤の探索を行う「再生研究」の基盤技術の確立を行った。

  16. 磁性ナノテクノロジーによる骨格筋再生医療の技術基盤の創製

    2014.4 - 2017.3

    日本学術振興会  科学研究費助成事業  基盤研究(B)

    井藤 彰, 清水一憲

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    Authorship：Principal investigator  Grant type：Competitive

    本研究では、骨格筋再生医療における「再生治療」と「再生研究」に有用な新規技術基盤の開発を行った。特に、我々がこれまで開発してきた先進医工学技術「磁性ナノテクノロジー」を効果的に利用し、独創的な方法論で研究展開をはかった。骨格筋は組織を形成して初めて本来の機能を発揮する。磁力を用いた組織工学技術により、電気刺激によって「動く」筋組織を作製することで、移植組織の「質」を評価する「再生治療」の評価基盤の確立を行った。また、再生治療研究として、ヒトiPS細胞の筋分化法の検討を行った。さらに、微細加工技術を組み合わせることで筋組織アレイを開発し、新規薬剤の探索を行う「再生研究」の基盤技術の確立を行った。

  17. 磁気細胞操作技術による高速3次元細胞システム構築

    2014.4 - 2016.3

    科学研究費補助金 

    井藤　彰

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    本研究は、磁性ナノ粒子と磁力を用いることで、「遺伝子修飾工程」と「三次元組織構築工程」のプロセスを高速化し、それらをまとめて一連の「磁気細胞操作技術による高速3次元細胞システム」を完成することを目的に行った。
    1)「遺伝子修飾工程」の開発成果：磁力を用いた遺伝子導入法「マグネトフェクション法」の開発を行った。正電荷脂質包埋型磁性ナノ粒子を作製し、これをレトロウイルスベクターに添加して、iPS細胞に磁力で引き寄せることで、磁力なしの場合と比較して、約4倍 遺伝子導入効率が上昇した。また、遺伝子回路構築および遺伝子導入細胞構築において、以下の成果を挙げた。1.交流磁場をトリガーにして遺伝子発現するシステムの構築：熱誘導型HSP70B’プロモーターを用いて、Tet-Onシステムと組み合わせることで、交流磁場による磁性ナノ粒子の発熱をトリガーにして目的遺伝子を持続的に高発現するシステムを構築した。再生医療への用途のため、発熱で細胞死が起こらないレベルの発熱であっても、本システムを使用すると、目的遺伝子が持続的に高発現することを実証した。2.組織を構築した際に低酸素になった部分が遺伝子発現するシステムの構築：低酸素誘導型RTP801プロモーターを用いて、Tet-Onシステムと組み合わせることで、三次元組織内における低酸素環境下で目的遺伝子を高発現するシステムを構築した。
    2）「三次元組織構築工程」の研究成果
    三次元細胞システムの構築として、磁力を用いて遺伝子導入筋組織を作製し、機能する（強い収縮力を示す）三次元筋組織を構築した。筋分化を促進するIGF-I遺伝子と組織内部の低栄養/低酸素状態でのアポトーシスを阻害するBcl-2遺伝子を筋芽細胞に共導入することで、高筋分化かつ高細胞密度の三次元筋組織を作製することに成功した。

  18. 筋組織形成促進および筋萎縮抑制効果を有する素材の探索

    2014

    キリン株式会社 R&D本部 基盤技術研究所  企業からの受託研究 

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    Authorship：Principal investigator  Grant type：Competitive

    独自に開発したティッシュエンジニアリング技術で作製した三次元筋組織を用いて、収縮力を指標にしたサルコペニア抑制素材の検索を行った。

  19. バイオテクノロジーを駆使した人工筋組織による新原理アクチュエータの創製

    2011.4 - 2014.3

    科学研究費補助金  若手研究(A)

    井藤　彰

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    Authorship：Principal investigator 

    生体の筋肉に近い性質および性能をもつ動力素子（アクチュエータ）はロボット工学などの分野に新しい展開を生み出すと考えられる。我々は、磁力を用いた三次元組織構築技術（Mag-TE法）を利用して、高密度で配向した筋芽細胞からなる三次元組織を構築し、さらに遺伝子導入および電気刺激培養により機能を高めて、電気刺激に応じて収縮運動する人工筋組織（バイオアクチュエータ）を構築することに成功した。

  20. 機能性磁性ナノ粒子を用いた筋組織再生技術によるバイオアクチュエーターの開発

    2009.4 - 2011.3

    科学研究費補助金  若手研究(A)

    井藤　彰

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    Authorship：Principal investigator 

    筋肉はきわめて高いエネルギー効率を有する動力素子(アクチュエータ)である。本研究では、磁力を用いた組織工学技術を応用して、高密度の筋芽細胞からなる三次元組織を構築し、電気刺激に応じて収縮運動する人工筋組織を構築することに成功した。さらに、人工筋組織の高機能化を目指して、筋芽細胞への遺伝子導入を行い、機能向上を評価した。

  21. 細胞の磁気操作による三次元複合臓器構造体の構築

    2008.4 - 2010.3

    科学研究費補助金  特定領域研究

    井藤　彰

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    Authorship：Principal investigator 


    次世代の生体組織構築技術におけるキーテクノロジーになると考えられる細胞の操作技術について、磁性ナノ粒子を用いた磁気細胞操作の開発を行い、磁力を用いた再生医工学技術(Mag-TE法)を開発することを目的とした。主に以下の点に関して研究を行った。
    1. 磁力を用いたVEGF遺伝子導入細胞シートの作製と機能評価
    筋芽細胞株C2C12細胞に血管新生を誘導するサイトカインであるVEGFの遺伝子を導入し、さらに正電荷脂質包埋型磁性ナノ粒子(MCL)を用いてC2C12細胞を磁気標識し、磁力によって細胞を積層化することによってVEGF遺伝子導入細胞シートを作製した。作製した細胞シートは、VEGFが分泌されていることをウエスタンブロット法で確認し、さらに導入したVEGFが正常に機能することを、血管内皮細胞の遊走および増殖・毛細血管形成能で確認した。さらに本方法で作製した遺伝子導入細胞シートをヌードマウスの皮下に移植することで、VEGF遺伝子導入の効果について検証した。移植した組織は、VEGF遺伝子を導入しなかった対照群と比較して有意に厚い組織を形成し、また、血管内皮特異的マーカー抗体による染色によって、血管内皮細胞や毛細血管の組織内に占める割合が有意に増加していることが分かった。これらの結果から、VEGF遺伝子導入細胞シートは移植に有用である可能性が示された。
    2. 磁力を用いた抗菌ペプチド遺伝子導入表皮細胞シートの作製と機能評価
    表皮角化細胞株HaCaT細胞に抗菌ペプチドHBD-3の遺伝子を導入し、Mag-TE法で細胞を積層化することによってHBD-3遺伝子導入細胞シートを作製した。作製した細胞シートは大腸菌に対する抗菌活性を示したことから、本アプローチは再生医療に有用である可能性が示された。

  22. ナノバイオ磁性ナノ粒子を用いたマルチスケールバイオマニピュレーション

    2007.4 - 2009.3

    科学研究費補助金  若手研究(A)

    井藤　彰

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    Authorship：Principal investigator 

    0nm程の直径の磁性ナノ粒子を、様々なバイオマテリアルで修飾することで、ナノバイオ磁性ナノ粒子を開発し、磁力を用いて細胞を操作する技術を開発した。具体的には、(1)レトロウイルスベクターを磁気操作する技術、(2)共培養後の標的細胞を磁気分離する技術、(3)細胞を磁力で配置するパターニング技術、(4)細胞を磁力で集積させて高度な三次元組織を構築する技術、といったナノからミリメーターに及ぶマルチスケールバイオマニピュレーションの開発を行った。

  23. 細胞の磁気操作による毛細血管網を含む生体組織の構築

    2006.4 - 2008.3

    科学研究費補助金  特定領域研究

    井藤　彰

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    1.磁力を用いた肝実質細胞一線維芽細胞からなる共培養細胞シートの構築
    線維芽細胞(NIH3T3細胞)と肝癌細胞(HepG2細胞)に磁性ナノ粒子を取り込ませて磁気ラベルし、それぞれの細胞を、磁石を設置した超低接着性培養皿に播種した。播種された細胞は、培養皿底面に接着することができないために、細胞間で結合・接着をはじめて、NIH3丁3細胞とHepG2細胞からなる共培養三次元組織を形成した。この三次元共培養により、HepG2の肝機能の一つであるアルブミン分泌能が向上したことから、我々の開発した「磁力を用いる細胞の磁気操作法」により、機能する三次元組織の構築に成功した。
    2.血管内皮細胞がパターン化された細胞シートの構築
    磁力を用いた細胞のパターニング技術を基盤にして、血管内皮細胞を含む細胞シートの構簗法の検討を行った。具体的には、(1)磁力を用いて線維芽細胞シートおよび筋芽細胞シートを作製し、(2)磁石をマイクロパターン化磁石(幅200μm)に取り替えて、細胞シートの上に磁性ナノ粒子で磁気標識した血管内皮細胞を播種した。結果として、それぞれの細胞シート上に、磁石の形状と同様の200μm線状に血管内皮細胞をパターンすることに成功した。このことから、我々の開発した「磁力を用いる細胞の磁気操作法」により、パターン化された血管内皮細胞を含む三次元組織の構築に成功した。
    以上の結果より、磁力を用いる細胞の磁気操作法は、ティッシュエンジニアリングにおける有用な技術であると考えられる。

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Industrial property rights 9

  1. 凍結保護液及び凍結保存方法

    井藤彰、金子真大、若林大誠

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    Applicant：名古屋大学

    Application no：特願2023-137200   Date applied：2023.8

  2. MAGNETIC-FIELD GENERATOR FOR A CELL SHEET

    Kotaro HIRAYAMA, Hotaka KAYANO, Shota TANOUE, Yuki WATANABE, Rei SHIBATA, Toshio KOKURYO, Akira ITO, Toyoaki MUROHARA

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    Application no：63/011,001  Date applied：2020.4

  3. 多能性幹細胞の融解方法

    井藤　彰

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    Applicant：国立研究開発法人科学技術振興機構

    Application no：特願2020-001413  Date applied：2020.1

    Announcement no：WO2021/140726A1  Date announced：2021.7

    Country of applicant：Domestic  

  4. 細胞培養方法及び培養組織

    井藤彰、本多裕之、小林猛、畠賢一郎、各務秀明、上田実

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    Applicant：本多裕之、J-TEC

    Application no：特願2003-136336  Date applied：2003.5

    Country of applicant：Domestic  

  5. 医用材料

    小林猛、本多裕之、井藤彰、新海政重、間瀬敦則、瀧澤洋平

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    Applicant：（財）名古屋産業科学研究所

    Application no：特願2003-78964  Date applied：2003.3

    Country of applicant：Domestic  

  6. 細胞培養方法及び細胞シート

    井藤彰、本多裕之、小林猛、畠賢一郎、各務秀明、上田実

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    Applicant：本多裕之、J-TEC

    Application no：特願2003-73808  Date applied：2003.3

    Country of applicant：Domestic  

  7. 細胞培養方法

    井藤彰、本多裕之、小林猛、畠賢一郎、各務秀明、上田実

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    Applicant：本多裕之、J-TEC

    Application no：特願2003-46060  Date applied：2003.2

    Country of applicant：Domestic  

  8. Heat therapy agent of tumor comprising cytokine and magnetic particles

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    Application no：特願2003-46060  Date applied：2003

    Country of applicant：Foreign country  

  9. 熱ショックタンパク質と磁性微粒子からなる悪性腫瘍の温熱治療剤

    井藤彰、本多裕之、小林猛

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    Applicant：小林猛、TTC

    Date applied：2003

    Patent/Registration no：WO2004087208A1 

    Country of applicant：Domestic  

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Teaching Experience (On-campus) 16

  1. 流動

    2023

  2. 分離融合工学

    2023

  3. 医工連携セミナー

    2023

  4. 化学工学システム論

    2023

  5. マテリアル工学概論

    2023

  6. マテリアル工学演習

    2023

  7. マテリアル工学演習

    2020

  8. 医工連携セミナー

    2020

  9. 化学工学システム論

    2020

  10. マテリアル工学概論

    2020

  11. 混相流動

    2020

  12. 混相流動

    2019

  13. 流動および演習

    2019

  14. マテリアル工学概論

    2019

  15. 化学工学システム論

    2019

  16. 生物化学工学

    2019

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Teaching Experience (Off-campus) 8

  1. 化学工学

    2020.9 （Meijo University）

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    Level：Undergraduate (specialized) 

  2. マテリアル工学概論

    2019.6 （Nagoya University）

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    Level：Undergraduate (specialized) 

  3. マテリアル工学演習

    （Nagoya University）

  4. 生物化学工学

    （Nagoya University）

  5. 混相流動

    （Nagoya University）

  6. 流動および演習

    （Nagoya University）

  7. 医工連携セミナー

    （Nagoya University）

  8. 化学工学システム論

    （Nagoya University）

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Social Contribution 4

  1. 第1回高等研究院ウェビナー（高等研究院ｘ未来社会創造機構）

    Role(s)：Panelist,　Presenter,　Planner,　Organizing member

    名古屋大学  2020.6

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    Audience： Graduate students,　Teachers,　Researchesrs,　General,　Scientific,　Company

    Type：Seminar, workshop

  2. 第1回高等研究院ウェビナー（高等研究院ｘ未来社会創造機構）

    Role(s)：Panelist,　Presenter,　Planner,　Organizing member

    名古屋大学  2020.6

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    Audience： Graduate students,　Teachers,　Researchesrs,　General,　Scientific,　Company

    Type：Seminar, workshop

  3. 日本学術会議若手アカデミー　公開ワークショップ

    Role(s)：Planner,　Organizing member

    日本学術会議若手アカデミー　  大分県別府市  2020.1

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    Audience： Graduate students,　Teachers,　Researchesrs,　General,　Civic organization,　Governmental agency

    Type：Seminar, workshop

  4. 日本学術会議若手アカデミー 公開ワークショップ

    Role(s)：Planner,　Organizing member

    日本学術会議若手アカデミー  大分県別府市  2020.1

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    Audience： Graduate students,　Teachers,　Researchesrs,　General,　Civic organization,　Governmental agency

    Type：Seminar, workshop

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Media Coverage 2

  1. 永井科学技術財団、研究助成決まる　財団賞13件・奨励金20件 Newspaper, magazine

    日刊工業新聞  2024.3

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    Author：Other 

  2. 奨励金など33件決定　永井科学技術財団が贈呈式 Newspaper, magazine

    中部経済新聞  2024.3

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    Author：Other 

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Academic Activities 1

  1. 日本学術会議連携会員 International contribution

    Role(s)：Planning/Implementing academic research

    日本学術会議  2009.11

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    Type：Scientific advice/Review 

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