Graduate School of Bioagricultural Sciences

Updated on 2023/04/03
博士(農学) ( 1997.3 東京大学 )
修士(農学) ( 1992.3 東京大学 )
Genetic engineering
Animal cell technology
遺伝子改変鳥類
Manufacturing Technology (Mechanical Engineering, Electrical and Electronic Engineering, Chemical Engineering) / Biofunction and bioprocess engineering / Engineering@Process Engineering@Bioengineering
Life Science / Applied molecular and cellular biology
CHO細胞を用いた物質生産プロセスの効率化
Regulation of immune responses by cellular lectins
Production of Transgenic birds
遺伝子改変ニワトリを用いたワクチン及び医薬品タンパク質生産システムの開発
Nagoya University Professor
2020.4
Nagoya University Associate professor
2012.4 - 2020.3
Department of Biotechnology, Nagoya University Assistant
1994.8
Country:Japan
The University of Tokyo Graduate School, Division of Agricultural Science Applied Biological Chemistry
- 1992
Country: Japan
The University of Tokyo Faculty of Agriculture Department of Applied Biological Chemistry
- 1990
Country: Japan
日本動物細胞工学会 庶務幹事
2011.6
日本生物工学会
日本農芸化学会
分子生物学会
日本免疫学会
化学工学会
日本生化学会
日本糖質学会
生物遺伝資源委員会 委員
2020.4
日本農芸化学会2018年度大会トピックス賞
2018.3 日本農芸化学会 培養始原生殖細胞の樹立とCRISPR/Cas9によるeGFPノックインニワトリの作製
萩原遥太、奥嵜雄也、金岡英徳、飯島信司、西島謙一
Wiley Blackwell Prize 2014
2014
第17回生物工学論文賞
2009 日本生物工学会
第15回生物工学論文賞
2007.9 社団法人日本生物工学会
日本動物細胞工学会奨励賞
2006.9 日本動物細胞工学会
Siglec-9 enhances IL-10 production in macrophages via tyrosine-based motifs Reviewed
Munetoshi Ando, Wenjie Tu, Ken-ichi Nishijima, and Shinji Iijima
Biochem. Biophys. Res. Commun. Vol. 369 page: 878-883 2008
New insights into the role of microheterogeneity of ZP3 during structural maturation of the avian equivalent of mammalian zona pellucida Reviewed
Hiroki Okumura, Ayaka Mizuno, Eri Iwamoto, Rio Sakuma, Shunsuke Nishio, Ken- ichi Nishijima, Tsukasa Matsuda, Minoru Ujita
PLOS ONE Vol. 18 ( 3 ) page: e0283087 2023.3
Regulatory mechanism of chicken lysozyme gene expression in oviducts examined using transgenic technology
Kojima Yusuke, Okuzaki Yuya, Nishijima Ken-ichi, Moriwaki Shuichiro, Asai Seiya, Kaneoka Hidenori, Iijima Shinji
JOURNAL OF BIOSCIENCE AND BIOENGINEERING Vol. 131 ( 4 ) page: 453 - 459 2021.4
Primordial germ cell-specific expression ofeGFPin transgenic chickens Reviewed
Hagihara Yota, Okuzaki Yuya, Matsubayashi Kazuma, Kaneoka Hidenori, Suzuki Takayuki, Iijima Shinji, Nishijima Ken-ichi
GENESIS Vol. 58 ( 8 ) 2020.8
Siglec-F is induced by granulocyte-macrophage colony-stimulating factor and enhances interleukin-4-induced expression of arginase-1 in mouse macrophages Reviewed
Tateyama Hiroyuki, Murase Yusuke, Higuchi Hiroshi, Inasaka Yui, Kaneoka Hidenori, Iijima Shinji, Nishijima Ken-ichi
IMMUNOLOGY Vol. 158 ( 4 ) page: 340 - 352 2019.12
PRDM14 and BLIMP1 control the development of chicken primordial germ cells Reviewed
Okuzaki Yuya, Kaneoka Hidenori, Suzuki Takayuki, Hagihara Yota, Nakayama Yuki, Murakami Seitaro, Murase Yusuke, Kuroiwa Atsushi, Iijima Shinji, Nishijima Ken-ichi
DEVELOPMENTAL BIOLOGY Vol. 455 ( 1 ) page: 32 - 41 2019.11
Identification of transgene integration site and anatomical properties of fluorescence intensity in a EGFP transgenic chicken line Reviewed
Tsujino Kaori, Okuzaki Yuya, Hibino Nobuyuki, Kawamura Kazuki, Saito Seiji, Ozaki Yumi, Ishishita Satoshi, Kuroiwa Atsushi, Iijima Shinji, Matsuda Yoichi, Nishijima Kenichi, Suzuki Takayuki
DEVELOPMENT GROWTH & DIFFERENTIATION Vol. 61 ( 7-8 ) page: 393 - 401 2019.9
Molecular cloning of chicken TET family genes and role of chicken TET1 in erythropoiesis Reviewed
Yuya Okuzaki, Hidenori Kaneoka, Ken-ichi Nishijima, Seitaro Murakami, Yuki Ozawa, Shinji Iijima
Biochemical and Biophysical Research Communications Vol. 490 ( 3 ) page: 753 - 759 2017.8
Expression of interferon-inducible transmembrane proteins in the chicken and possible role in prevention of viral infections Reviewed
Shunsuke Kidani, Yuya Okuzaki, Hidenori Kaneoka, Seiya Asai, Seitaro Murakami, Yusuke Murase, Shinji Iijima, Ken-ichi Nishijima
Cytotechnology Vol. 69 ( 3 ) page: 477 - 484 2017.6
Characterization of chicken interferon-inducible transmembrane protein-10 Reviewed
Yuya Okuzaki, Shunsuke Kidani, Hidenori Kaneoka, Shinji Iijima and Ken-ichi Nishijima
Bioscience, Biotechnology and Biochemistry Vol. 81 ( 5 ) page: 914 - 921 2017.5
Constitutively expressed Siglec-9 inhibits LPS-induced CCR7, but enhances IL-4-induced CD200R expression in human macrophages Reviewed
Hiroshi Higuchi, Toru Shoji, Shinji Iijima, Ken-ichi Nishijima
Bioscience, Biotechnology and Biochemistry Vol. 80 ( 6 ) page: 1141-1148 2016.6
Analyses of chicken sialyltransferases related to O-glycosylation Reviewed
Shunsuke Kidani, Yuya Okuzaki, Hidenori Kaneoka, Seiya Asai, Seitaro Murakami, Ken-ichi Nishijima, Shinji Iijima,
Journal of Bioscience and Bioengineering 2016.4
Siglec-9 modulated IL-4 responses in the macrophage cell line RAW264. Reviewed
Higuchi H, Shoji T, Murase Y, Iijima S, Nishijima K
Bioscience, Biotechnology and Biochemistry Vol. 80 ( 3 ) page: 501–509 2016.3
A soluble form of Siglec-9 provides a resistance against Group B Streptococcus (GBS) infection in transgenic mice Reviewed
Mitsumasa Saito, Sayo Yamamoto, Kinuyo Ozaki, Yukiko Tomioka, Haruka Suyama, Masami Morimatsu, Ken-ichi Nishijima, Shin-ichi Yoshida, Etsuro Ono
Microbial Pathogenesis Vol. 99 page: 106-110 2016
Analyses of chicken sialyltransferases related to N-glycosylation Reviewed
Yusuke Kojima, Akifumi Mizutani, Yuya Okuzaki, Ken-ichi Nishijima, Hidenori Kaneoka, Takako Sasamoto, Katsuhide Miyake, Shinji Iijima
Journal of Bioscience and Bioengineering Vol. 119 ( 6 ) page: 623-628 2015.6
Lectin-dependent localization of cell surface sialic acid-binding lectin Siglec-9 Reviewed
Munetoshi Ando, Toru Shoji, Wenjie Tu, Hiroshi Higuchi, Ken-ichi Nishijima, and Shinji Iijima
Cytotechnology Vol. 67 page: 601-608 2015
Enhanced lentiviral vector production in 293FT cells expressing Siglec-9 Reviewed
Toru Shoji, Hiroshi Higuchi, Yoshinori Zaitsu, Ken-ichi Nishijima and Shinji Iijima
Cytotechnology Vol. 67 page: 593-600 2015
Effects of Siglec on the expression of IL-10 in the macrophage cell line RAW264 Reviewed
Toru Shoji, Hiroshi Higuchi, Ken-ichi Nishijima and Shinji Iijima
Cytotechnology Vol. 67 page: 633-639 2015
Siglec-9 modulated IL-4 responses in the macrophage cell line RAW264 Reviewed
Hiroshi Higuchi, Toru Shoji, Yusuke Murase, Shinji Iijima, Ken-ichi Nishijima
Bioscience, Biotechnology and Biochemistry page: in press 2015
A soluble form of Siglec-9 provides an antitumor benefit against mammary tumor cells expressing MUC1 in transgenic mice Reviewed
Yukiko Tomioka, Masami Morimatsu, Ken-ichi Nishijima, Sayo Yamamoto, Haruka Suyama, Kinuyo Ozaki, Etsuro Ono
Biochem Biophys Res Commun Vol. 450 ( 1 ) page: 532-537 2014.6
Recombinant proteins produced into yolk of genetically manipulated chickens are partly sialylated in N-glycan Reviewed
Kazuhiro Yoshida, Yuya Okuzaki, Ken-ichi Nishijima, Kenji Kyogoku, Takashi Yamashita, Yoshinori Kawabe, Makoto Motono, Masamichi Kamihira, and Shinji Iijima
Cytotechnology 2013
Resistance to influenza A virus infection in transformed cell lines expressing an anti-PB2 monoclonal antibody Reviewed
Yoshikazu Fujimoto, Kinuyo Ozaki, Masahiro Maeda, Ken-ichi Nishijima, Hiroki Takakuwa, Koichi Otsuki, Hiroshi Kida, Etsuro Ono
Veterinary Journal Vol. 198 ( 2 ) page: 487-493 2013
Galactosylation of human erythropoietin produced by chimeric chickens expressing galactosyltransferase Reviewed
Yusuke Kojima, Jun Wakita, Yujin Inayoshi, Ryosuke Suzuki, Yuki Yamada, Hidenori Kaneoka, Ken-ichi Nishijima, Shinji Iijima
Journal of Bioscience and Bioengineering 2013
Chicken oviduct-specific expression of transgene by a hybrid ovalbumin enhancer and the Tet expression system Reviewed
Daisuke Kodama, Daisuke Nishimiya, Ken-ichi Nishijima, Yuki Okino, Yujin Inayoshi, Yasuhiro Kojima, Ken-ichiro Ono, Makoto Motono, Katsuhide Miyake, Yoshinori Kawabe, Kenji Kyogoku, Takashi Yamashita, Masamichi Kamihira and Shinji Iijima
Journal of Bioscience and Bioengineering Vol. 113 page: 146-153 2012
Genetic modification of a chicken expression system for the galactosylation of therapeutic proteins produced in egg white Reviewed
Akifumi Mizutani, Hiroyuki Tsunashima, Ken-ichi Nishijima, Takako Sasamoto, Yuki Yamada, Yasuhiro Kojima, Makoto Motono, Jun Kojima, Yujin Inayoshi, Katsuhide Miyake, Enoch Y. Park and Shinji Iijima
Transgenic Research Vol. 21 page: 63-75 2012
Moloney murine leukemia virus integrase and reverse transcriptase interact with PML proteins Reviewed
Yuuki Okino, Yujin Inayoshi, Yusuke Kojima, Shunsuke Kidani, Hidenori Kaneoka, Akiko Honkawa, Hiroshi Higuchi, Ken-ichi Nishijima, Katsuhide Miyake, and Shinji Iijima
Journal of Biochemistry 2012
Production of Transgenic Chickens from Purified Primordial Germ Cells Infected with a Lentiviral Vector Reviewed
Makoto Motono, Yuki Yamada, Yuki Hattori, Ryo Nakagawa, Ken-ichi Nishijima, and Shinji Iijima
Journal of Bioscience and Bioengineering Vol. 109 ( 4 ) page: 315-321 2010
Transcription factor YY1 interacts with retroviral integrases and facilitates integration of Moloney murine leukemia virus cDNA into the host chromosomes Reviewed
Yujin Inayoshi, Yuuki Okino, Katsuhide Miyake, Akifumi Mizutani, Junko Yamamoto-Kishikawa, Yuya Kinoshita, Yusuke Morimoto, Kazuhito Imamura, Mahboob Morshed, Ken Kono, Toshinari Itoh, Ken-ichi Nishijima, and Shinji Iijima
Journal of Virology Vol. 84 ( 16 ) page: 8250-8261 2010
Production of chimeric monoclonal antibodies by genetically manipulated chickens Reviewed
Masamichi Kamihira, Yoshinori Kawabe, Takuya Shindo, Ken-ichiro Ono, Kazuhisa Esaka, Takashi Yamashita, Ken-ichi Nishijima, and Shinji Iijima
Journal of Biotechnology Vol. 141 page: 18-25 2009
Production of human erythropoietin by chimeric chickens Reviewed
Daisuke Kodama, Daisuke Nishimiya, Ken-ichi Iwata, Kazuhisa Yamaguchi, Kazuhiro Yoshida, Yoshinori Kawabe, Makoto Motono, Hiroyuki Watanabe, Takashi Yamashita, Ken-ichi Nishijima, Masamichi Kamihira, and Shinji Iijima
Biochem. Biophys. Res. Commun. Vol. 367 page: 834-839 2008
Retroviral gene transduction into chicken embryo gonads through blood circulation Reviewed
Yoshinori Kawabe, Tsutomu Naka, Hiroyuki Komatsu, Ken-ichi Nishijima, Shinji Iijima, and Masamichi Kamihira
Journal of Bioscience and Bioengineering Vol. 106 ( 6 ) page: 598-601 2008
Analysis of chicken primordial germ cells Reviewed
Makoto Motono, Takuya Ohashi, Ken-ichi Nishijima, and Shinji Iijima
Cytotechnology Vol. 57 page: 199-205 2008
Production of Recombinant TNFR/Fc Fusion Protein by Genetically Manipulated Chickens Reviewed
Kenji Kyogoku, Kazuhiro Yoshida, Hiroyuki Watanabe, Takashi Yamashita, Yoshinori Kawabe, Makoto Motono, Ken-ichi Nishijima, Masamichi Kamihira, and Shinji Iijima
J. Biosci. Bioeng. Vol. 105 page: 454-459 2008
Chicken Ovalbumin Promoter is Demethylated upon Expression in the Regions Specifically Involved in Estrogen-Responsiveness Reviewed
Mahboob Morshed, Shusuke Sano, Daisuke Nishimiya, Munetoshi Ando, Ken-ichi Nishijima, and Shinji Iijima
Biosci. Biotech. Biochem Vol. 70 ( 6 ) page: 1438-1446 2006
Transport of human immunoglobulin G and Fc-fusion proteins to chicken egg yolk Reviewed
Yoshinori Kawabe, Tsutomu Naka, Naoko Ando-Noumi, Hiroyuki Matsumoto, Ken-ichiro Ono, Ken-ichi Nishijima, Masamichi Kamihira, and Shinji Iijima
J. Biosci. Bioeng. Vol. 102 ( 6 ) page: 518-523 2006
Production of scFv-Fc Fusion Protein by Genetically Manipulated Quails Reviewed
Yoshinori Kawabe, Masamichi Kamihira, Ken-ichiro Ono, Kenji Kyogoku, Ken-ichi Nishijima, and Shinji Iijima
J. Biosci. Bioeng. Vol. 102 ( 4 ) page: 297-303 2006
YY1 binds to regulatory element of chicken lysozyme and ovalbumin promoters Reviewed
Mahboob Morshed, Munetoshi Ando, Junko Yamamoto, Akitsu Hotta, Hidenori Kaneoka, Jun Kojima, Ken-ichi Nishijima, Masamichi Kamihira and Shinji Iijima
Cytotechnology Vol. 10.1007/s10616-006-9017-4 2006
Characterization of transient expression system for retroviral vector production Reviewed
Akitsu Hotta, Yoshikazu Saito, Kenji Kyogoku, Yoshinori Kawabe, Ken-ichi Nishijima, Masamichi Kamihira, Shinji Iijima
J. Biosci. Bioeng Vol. 101 ( 4 ) page: 361-368 2006
High level expression of single-chain Fv-Fc fusion protein in serum and egg white of geneticallu manipulated chickens using a retroviral vector Reviewed
Masamichi Kamihira, Ken-ichiro Ono, Kazuhisa Esaka, Ken-ichi Nishijima, Ryoko Kigaku, Hiroyuki Komatsu, Takashi Yamashita, Kenji Kyogoku and Shinji Iijima
Journal of Virology Vol. 79 ( 17 ) page: 10864-10874 2005
Costimulation of T cell proliferation by anti-L-selectin antibody is associated with the reduction of a cdk inhibitor p27 Reviewed
Ken-ichi Nishijima, Munetoshi Ando, Shusuke Sano, Aiko Hayashi-Ozawa, Yoshinori Kinoshita
Immunology Vol. 116 page: 347-353 2005
Production of anti-CD2 chimeric antibody by recombinant animal cells Reviewed
Akitsu Hotta, Masamichi Kamihira, Mahboob Morshed, Kanako Itoh, Yoshinori Kawabe, Ken-ichiro Ono, Hiroyuki Matsumoto, Ken-ichi Nishijima and Shinji Iijima
J. Biosci. Bioeng. Vol. 98 ( 4 ) page: 298-303 2004
Production of anti-prion scFv-Fc fusion proteins by recombinant animal cells Reviewed
Kenichiro Ono, Masamichi Kamihira, Yuko Kuga, Hiroyuki Matsumoto, Akitsu Hotta, Toshinari Itoh, Ken-ichi Nishijima, Naoto Nakamura, Haruo Matsuda and Shinji Iijima
J. Biosci. Bioeng. Vol. 95 ( 3 ) page: 231-238 2003
Simple assay method for endocrine disrupters by in vitro quail embryo culture: nonylphenol acts as a weak estrogen in quail embryos Reviewed
Ken-ichi Nishijima, Kazuhisa Esaka, Hisayo Ibuki, Kenichiro Ono, Katsuhide Miyake, Masamichi Kamihira and Shinji Iijima
J. Biosci. Bioeng. Vol. 95 ( 6 ) page: 612-617 2003
Production of transgenic quails with high frequency of germ-line transmission using VSV-G pseudotyped retroviral vector Reviewed
Shinji Mizuarai, Kenichiro Ono, Kazuhisa Yamaguchi, Ken-ichi Nishijima, Masamichi Kamihira and Shinji Iijima
Biochem. Biophys. Res. Commun. Vol. 286 ( 3 ) page: 456-463 2001
The effects of cell adhesion on the growth and protein productivity of animal cells Reviewed
Ken-ichi Nishijima, Tsukasa Fujiki, Hirotoshi Kojima and Shinji Iijima
Cytotechnology Vol. 33 page: 147-155 2000
Integrase-mediated nonviral gene transfection with enhanced integration efficiency Reviewed
Journal of Bioscience and Bioengineering Vol. 88 ( 5 ) page: 461-467 1999
Amplification of competitive telomere sequence in living animal cells induces chromatin instability Reviewed
Shinji Mizuarai, Ken-ichi Nishijima and Shinji Iijima
Cytotechnology Vol. 31 page: 193-201 1999
Induction of oral tolerance in splenocyte-reconstituted SCID mice Reviewed
Hiroko Yoshida, Satoshi Hachimura, Kazuki Hirahara, Tatsuhiro Hisatsune, Ken-ichi Nishijima, Akio Shiraishi and Shuichi Kaminogawa
Clinical Immunology and Immunopathology Vol. 87 ( 3 ) page: 282-291 1998
Oral administration of antigen does not influence the proliferation and IFN-α production of responsive CD8+ T cells but enables to establish T cell clones with different lymphokine production profile Reviewed
Ken-ichi Nishijima, Tatsuhiro Hisatsune, Hiroko Kato, Masako Kohyama, Masahiro Kakehi, Satoshi Hachimura and Shuichi Kaminogawa
Cytotechnology Vol. 25 page: 89-100 1997
Enhancing effect of interleukin-4 on the secretion of interferon-γ by αs1-casein-specific CD8+ T cells. Reviewed
Ken-ichi Nishijima, Masako Kohyama, Tatsuhiro Hisatsune, Hiroko Kato, Masahiro Kakehi and Shuichi Kaminogawa
Bioscience, Biotechnology and Biochemistry Vol. 61 ( 7 ) page: 1156 1997
食餌抗原に応答して抑制性リンフォカインを産生するCD8+T細胞 Invited
西島謙一、上野川修一
医学の歩み Vol. 177 ( 5 ) page: 364-367 1996
Difference in signal transduction for IL-10 and IFN-r production in a DC8+ T cell clone. Reviewed
Yuji Minai, Masao Goto, Masako Kohyama, Tatsuhiro Hisatsune, Ken-ichi Nishijima and Shuichi Kaminogawa
Cellular Immunology Vol. 172 ( 2 ) page: 200 1996
CD8+ T cells specific to the exogenous antigen. Mode of antigen recognition and possible implicationin immunosuppression Reviewed
Tatsuhiro Hisatsune, Ken-ichi Nishijima, Masako Kohyama, Hiroko Kato and Shuichi Kaminogawa
The Journal ofImmunology Vol. 154 page: 88 1995
αS1-casein-specific CD8+ T cell clone that inhibits its own interferon-gamma production by producing interleukin-10 Reviewed
Ken-ichi Nishijima, Tatsuhiro Hisatsune, Hiroko Kato, Osamu Shiho and Shuichi Kaminogawa
Bioscience, Biotechnology, and Biochemistry Vol. 59 ( 12 ) page: 2274-2276 1995
CD4+ T cells anergized by high-dose feeding establish oral tolerance to antibody responses when transfected in SCID and nude mice Reviewed
Kazuki Hirahara, Tatsuhiro Hisatsune, Ken-ichi Nishijima, Hiroko Kato, Osamu Shiho and Shuichi Kaminogawa
Journal of Immunology Vol. 154 page: 6238-6245 1995
Anti-IL-10 antibodyenhances the proliferation of CD8+ Tcell clones : autoregulatory role of murineIL-10 in CD8+ T cells Reviewed
Ken-ichi Nishijima, Tatsuhiro Hisatsune, Yuji Minai, Masako Kohyama and Shuichi Kaminogawa
Cellular Immunology Vol. 154 page: 193 1994
Autoreactive CD8+ T cell clones producing immunesuppressive lymphokines IL-10 andinterferon-γ Reviewed
Tatsuhiro Hisatsune, Ken-ichi Nishijima, Yuji Minai, Masako Kohyama and Shuichi Kaminogawa
Cellular Immunology Vol. 154 page: 181 1994
Activation via TCR of IL-2 receptor of a CD8+ suppressor T cell clone: Effect on IL-10 production and on proliferation of suppressor T cell Reviewed
Yuji Minai, Tatsuhiro Hisatsune, Ken-ichi Nishijima, Atsushi Enomoto and Shuichi Kaminogawa
Cytotechnology Vol. 14 page: 81-87 1994
A suppressive lymphokine derived from Ts clone 13G2 is IL-10 Reviewed
Tatsuhiro Hisatsune, Yuji Minai, Ken-ichi Nishijima, Atsushi Enomoto, Kevin W. Moore, Takashi Yokota, Ken-ichi Arai and Shuichi Kaminogawa
Lymphokine and Cytokine Research Vol. 11 page: 87 1992
Strong influence ofthe processing of the antigen on negativeeffects on T cell activation by regions outsidethe determinant area of bovine αS1-casein Reviewed
Tatsuhiro Hisatsune, Akio Ametani, Ken-ichi Nishijima, Atsushi Enomoto and Shuichi Kaminogawa
Bioscience, Biotechnology Biochemistry Vol. 56 page: 1616 1992
CD8+ suppressorT cell clone capable of inhibiting the antigen- and anti-T cell receptor-induced proliferation of Th clones without cytolytic activity Reviewed
Tatsuhiro Hisatsune, Atushi Enomoto, Ken-ichi Nishijima, Yuji Minai, Yoshihiro Asano, Tomio Tada and Shuichi Kaminogawa,
The Journal ofImmunology Vol. 145 page: 2421 1990
Transgenic chickens Reviewed
Ken-ichi Nishijima and Shinji Iijima( Role: Joint author)
Development, Growth & Differentiation 2013
物質生産能の高い育種法の開発と活用
西島謙一( Role: Contributor , 第2章 第7節)
技術情報協会 2022.11 ( ISBN:978-4-86104-904-0 )
シグレックと免疫制御 Reviewed
西島謙一( Role: Contributor)
糖鎖生物学. 北島健、佐藤ちひろ、門松健治、加藤晃一編 名古屋大学出版会 2020.2
糖鎖から見たインフルエンザの適応変異 Reviewed
西島謙一( Role: Contributor)
化学と生物 2019.3
動物細胞がin vitro培養可能になったころ Reviewed
西島謙一( Role: Sole author , 続・生物工学基礎講座 バイオよもやま話)
日本生物工学会誌 2016.12
シアル酸による生体機能調節
西島謙一( Role: Sole author)
JAACT Newsletter 2014.4
タンパク質の次は糖鎖の時代になるの? Reviewed
西島謙一( Role: Sole author)
化学同人 2012.10
遺伝子組換え鳥類の作製と抗体生産技術 Reviewed
西島謙一( Role: Sole author)
CMC出版 「バイオ医薬品製造の効率化と生産基材の開発」 2012.4
ニワトリ卵白に生産させた医薬用タンパク質の糖鎖制御 Reviewed
西島謙一( Role: Sole author)
バイオインダストリー協会 2011
レトロウイルスベクターによる遺伝子導入JAACT Newsletter
西島謙一( Role: Sole author)
日本動物細胞工学会 2010
遺伝子導入鳥類の卵中への医薬品糖タンパク質生産 Reviewed
西島謙一( Role: Sole author)
学会出版センター 2009
Sterilization of chicken primordial germ cells
Makoto Motono, Hiroyuki Komatsu, Yoshinori Kawabe, Ken-ichi Nishijima, and Shinji Iijima( Role: Joint author)
Springer Publishers 2008
Immune regulation by sialoglycoconjugate-binding lectins t cell costimulation by L-selectin
Munetoshi Ando, Ken-ichi Nishijima, Shusuke Sano, Aiko Hayashi, Yoshinori Kinoshita, and Shinji Iijima( Role: Joint author)
Springer Publishers 2005
Production of chimeric antibodies by transgenic chicken bioreactors
Yoshinori Kawabe, Akitsu Hotta, Ken-Ichiro Ono, Kazuhisa Esaka, Ken-Ichi Nishijima, Masamichi Kamihira, and Shinji Iijima( Role: Joint author)
Springer Publishers 2005
Biochemical analysis of chicken ovalbumin promoter
Mahboob Morshed, Junko Yamamoto, Shusuke Sano, Ken-Ichi Nishijima, Masamichi Kamihira, and Shinji Iijima( Role: Joint author)
Springer Publishers 2005
Preparation of high-titer retroviral vectors using transient expression system
Akitsu Hotta, Yoshikazu Saito, Ken-ichi Nishijima, Msamichi Kamihira, and Shinji Iijima( Role: Joint author)
Springer Publishers 2005
"Production of pharmaceutical proteins by transgenic avian", in "Animal Cell Technology: Basic & Applied Aspects vol. 13" (Yagasaki, K. et al., Eds.)
Akitsu Hotta, Ken-ichiro Ono, Shinji Mizuarai, Kazuhisa Yamaguchi, Ken-ichi Nishijima, Masamichi Kamihira and Shinji Iijima,( Role: Joint author)
Kluwer Academic Publishers 2004
"Transgenic birds for the production of recombinant proteins" in Adv Biochem Engin/Biotechnol
Masamichi Kamihira, Ken-ichi Nishijima, Shinji Iijima( Role: Joint author)
Springer-Verlag 2004
”金の卵を産むニワトリをつくる トランスジェニック鳥類を使った有用タンパク質の生産”、化学と生物 Reviewed
上平正道、西島謙一、飯島信司( Role: Joint author)
学会出版センター 2003
”サイトカイン”、’食品と体 免疫・アレルギーのしくみ'(上野川修一編集) Reviewed
西島謙一( Role: Joint author)
朝倉書店 2003
”敵か味方か?:拒絶と受容を分ける免疫系の鍵分子”、生物工学会誌 Reviewed
西島謙一( Role: Sole author)
日本生物工学会 2003
”動物培養細胞”、’生物化学実験法45 組換えタンパク質生産法(塚越規弘編著)’ Reviewed
飯島信司、上平正道、西島謙一( Role: Joint author)
学会出版センター 2001
Effect of cell adhesion on the growth of animal cells
Hirotoshi Kojima, Ken-ichi Nishijima and Shinji Iijima( Role: Joint author)
Recent Developments in Biochemical Engineering 1998
Suppressive lympholines produced by CD8+ T cells
( Role: Joint author)
Animal Cell Technology: Basic & Applied Aspects. 1994
Mechanism of IL-10 poroduction by a CD8+ T cell clone
Yuji Minai, Tatsuhiro Hisatsune, Ken-ichi Nishijima, Atsushi Enomoto and Shuichi Kaminogawa( Role: Joint author)
Animal Cell Technology: Basic & Applied AspectsKluwer Academic Publishers, Netherlands 1993
CD8+ suppressor T cell clone 13G2 secretes a suppressive lymphokine, immune suppressive factor-T (ISF-T)
( Role: Joint author)
Animal Cell Culture and Production of Biologicals 1991
Chicken/Quail resources in Avian Bioscience Research Center in Nagoya University Invited International conference
K. Nishijima
Animal Genomics and Genome Project 2021.11.25
ニワトリ始原生殖細胞の 遺伝子改変への応用 Invited
西島謙一
基盤技術整備プログラム成果発表ワークショップ
Genetically Manipulated Chickens as Animal Bioreactor. Invited International conference
Nishijima, K.; Kaneoka, H.; Iijima, S
2016 World Congress on In Vitro Biology
Toward the understanding of transgenesis property of chicken PGCs International conference
Ken-ichi Nishijima
International Forum on Avian Germplasm 2013
Siglec-9 inhibits innate immunity International conference
Sialoglycoscience 2006, Fifth International Conference
Production of Antibody by Transgenic Chicken. International conference
2004 World Congress on In Vitro Biology
遺伝子改変鶏を利用した新規ワクチン生産プラットフォーム
2021.4 - 2024.3
善き未来を拓く科学技術
Authorship:Principal investigator Grant type:Competitive
( Direct Cost: \25000000 )
ニワトリ糖鎖制御技術の開発と炎症調節への展開
2018.4 - 2019.3
水谷糖質科学振興財団 水谷糖質科学振興財団 第25回研究助成
西島謙一
Authorship:Principal investigator Grant type:Competitive
( Direct Cost: \3000000 )
新規高機能インフルエンザワクチン生産法の確立
2012.4 - 2013.3
公益財団法人豊田理化学研究所 平成24年度豊田理研スカラー
Grant type:Competitive
炎症を制御するシアル酸認識レクチンの機能解析
2011.12 - 2012.12
財団法人テルモ科学技術振興財団 2011年度一般研究助成
Grant type:Competitive
トランスジェニックニワトリを用いた高機能インフルエンザワクチン生産法
2011.12 - 2012.3
財団法人中部科学技術センター 学術奨励研究助成金
Grant type:Competitive
シアル酸結合レクチンによる食餌性脂質作用の調節
2009
三島海雲記念財団
Grant type:Competitive
細胞表面シアル酸制御を介した自然免疫調節
2007
財団法人武田科学振興財団2007年度一般研究奨励
Grant type:Competitive
トランスジェニック鳥類による糖鎖改変タンパク質の生産
2007
財団法人旭硝子財団平成19年度採択研究助成
Grant type:Competitive
遺伝子導入鳥類の卵中への有用蛋白質生産技術
2002.10 - 2005.9
国内共同研究
トランスジェニック鳥類を用いたタンパク質性生理活性物質の大量生産法を技術開発を目指した。その結果、モデルタンパク質で卵一個あたり最大200mgもの高い生産性を得ることが出来た。
Grant number:22H02497 2022.4 - 2026.3
Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)
Authorship:Coinvestigator(s)
デザイン型育種プロセスの創成とインフルエンザ抵抗性ニワトリ作製への展開
Grant number:21K18186 2021.7 - 2024.3
科学研究費助成事業 挑戦的研究(開拓)
西島 謙一, 西藤 岳彦, 内田 裕子, 佐久間 咲希
Authorship:Principal investigator
Grant amount:\26000000 ( Direct Cost: \20000000 、 Indirect Cost:\6000000 )
鳥インフルエンザは世界中に蔓延している。特に、高病原性鳥インフルエンザは高い致死性と強い伝播性から、発生農場の全家禽の殺処分等の防疫措置をとる必要がある。本研究では、鶏が本来持つインフルエンザ耐性遺伝子を新規に同定することを目指す。最終的に、現在の商用鶏の高病原性トリインフルエンザに対する高い感受性を改善した新たな鶏系統を作出することを目指す。発見したインフルエンザ耐性遺伝子をコマーシャル系統に導入することができれば、鳥インフルエンザ蔓延防止と食糧の安定供給に大きく貢献できる。また、鳥インフルエンザは変異ウイルスがヒトのパンデミックを起こしてきた歴史があり、公衆防疫上も波及効果は絶大である。
Development of integrated animal cell engineering system for biopharmaceutical production
Grant number:20H00322 2020.4 - 2024.3
Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (A)
Authorship:Coinvestigator(s)
Grant number:20H02537 2020.4 - 2024.3
科学研究費助成事業 基盤研究(B)
西島 謙一, 小野 悦郎, 金岡 英徳
Authorship:Principal investigator
Grant amount:\17680000 ( Direct Cost: \13600000 、 Indirect Cost:\4080000 )
世界的流行の恐れから、インフルエンザワクチン生産法の抜本的改善が急がれている。発育鶏卵による従来法はワクチン生産に適した自動化機器類などが整備されており、今後もワクチンの大部分はこの方法により生産されるものと考えられる。本申請では、これまで開発してきた家禽の遺伝子操作技術を用いて新たなトランスジェニックニワトリを作製し、高力価なヒトインフルエンザワクチンを生産する新たな手法を提供することを目指す。本申請ではこのための基盤技術として精子を持たないニワトリ系統の作製も行う。ニワトリはワクチン生産だけでなく最も身近な家畜でもあり、本研究が完成されれば社会生活に対する影響は大きい。
発育鶏卵でインフルエンザウイルスの増殖を左右するのは漿尿膜上に存在する糖鎖の結合パターンであり、理論上ニワトリ型の糖鎖をヒト型化できれば、ヒトウイルスを馴化プロセスなしで増やせるようになる。そこで、本申請ではニワトリの遺伝子改変技術を用いてヒト型糖鎖発現ニワトリを作製することを目指す。安全性を確保するために2系統の遺伝子改変ニワトリを作成する計画であるが、本年度はこの2系統の遺伝子改変始原生殖細胞株を作製しレシピエント胚に移植することで生殖腺キメラを作製した。一部は成熟したため、遺伝子改変された次世代のニワトリを得るために交配とスクリーニングを始めている。この過程で、シアル酸転移酵素の発現がニワトリ細胞の増殖にマイナスに作用することを見いだした。増殖抑制の強度はそれほど大きくないため、発現により発生に悪影響を及ぼす可能性は低いものと考えられたが、この点についてはさらに検討を行う必要がある。組織特異的な発現が必要になる可能性もあり、そのためのモデルとしてリゾチームプロモーターの解析を行った。
また、我々が着目してきたPRDM14遺伝子のニワトリ始原生殖細胞における役割を確認するために、PRDM14を誘導発現可能なコンストラクトを導入する一方、内在性のPRDM14遺伝子を両アレルとも破壊した始原生殖細胞株を作製した。PRDM14の発現量は期待通り制御できたが、調べた範囲で始原生殖細胞の増殖及び遺伝子発現に影響はなかった。実験システムの特性により低レベルのバックグラウンド発現が残るが、この発現により細胞の性質が維持されたものと推定された。
生殖腺キメラについては概ね計画通り作製できている。新所属における遺伝子改変ニワトリの作製作業が軌道に乗りつつあり、交配個体数をさらに増やすことで計画した系統作製は可能であると考えられる。
引き続き生殖腺キメラを作製し交配を精力的に進める予定である。また、始原生殖細胞の維持に必要な遺伝子の解析については対象を広げBLIMP1など他の遺伝子についても検討を行う。
Integrated platform for mammalian cell-based cell and bioprocess engineering
Grant number:17H06157 2017.5 - 2022.3
Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (S)
Authorship:Coinvestigator(s)
Avian transgenic technology against influenza
Grant number:16H04574 2016.4 - 2020.3
Nishijima Ken-ichi
Authorship:Principal investigator
Grant amount:\17550000 ( Direct Cost: \13500000 、 Indirect Cost:\4050000 )
It was shown that chicken IFITM10 slightly reduced the infectivity of VSV-G-pseudotyped virus. In addition, overexpression of Muc13, of which gene size is below the limit to construct an expression vector, did not prevent infectivity of VSV-G-pseudotyped virus. Thus, expression system for large size mucin is under construction.
We examined the gene transfer and genome editing conditions suitable for the long-term cultured primordial germ cells. Using CRISPR/Cas9, we succeeded in producing a genome-edited chicken in which the EGFP gene was knocked in.
トランスジェニック技術を用いたニワトリ始原生殖細胞形成機構の解析
Grant number:16H01253 2016.4 - 2018.3
新学術領域研究(研究領域提案型)
西島 謙一
Authorship:Principal investigator
Grant amount:\9880000 ( Direct Cost: \7600000 、 Indirect Cost:\2280000 )
マウスにおいて始原生殖細胞分化のマスターレギュレーターとされるPrdm14に着目している。ニワトリ培養始原生殖細胞にPrdm14のsiRNAをエレクトロポレーションにより導入したところ、CvhやDazlの発現には影響を与えず、NANOGの発現が若干低下することが認められた。昨年度検討した胚盤葉細胞の過剰発現系とは異なる結果であった。この原因については現在のところ不明であるが、細胞の種類や分化段階などの違いが影響する可能性を考えている。
FGFから活性化されるMEK/MAPKシグナルを阻害したところ、Prdm14の発現が大きく低下することが示された。一方、Activin下流のSMADの阻害剤添加ではPrdm14の発現低下は12時間よりも遅くゆっくりと起こることが示された。これらのことから、培養始原生殖細胞ではMEK/ERK経路がPrdm14の発現に重要であることが示唆された。
一方、CRISPR/Cas9によるゲノム編集を試みたところ、培養始原生殖細胞にeGFP遺伝子をノックインすることに成功した。この細胞をレシピエント胚に移植したところ、高効率で生殖腺に定着することが確認された。成熟したオス個体ではeGFPノックイン細胞由来精子が多く存在し、野生型メスとの交配によりeGFPを持つ子孫を得ることに成功した。今後、このニワトリの解析を進める予定である。
DNA脱メチル化に関わるニワトリTET1を解析した。酵素活性は認められたものの、PGCでの発現は低く、ほ乳類と異なりPGCの初期分化過程においてDNAメチル化に大きな変化はないものと考えられた。一方、胚赤血球においてTET1が高発現していることが示され、赤血球分化に脱メチル化が関わっていることが示唆された。
29年度が最終年度であるため、記入しない。
29年度が最終年度であるため、記入しない。
Fundamental research for genome-wide platform of industrial mammalian cells
Grant number:26249125 2014.4 - 2018.3
Omasa Takeshi
Authorship:Coinvestigator(s)
The fundamental research of chromosome stability and selection of targeting region of genome, and analysis of signaling pathway for Chinese hamster ovary cells are necessary for genome-wide platform of industrial mammalian cells. We focused on the chromosome stability and find that BRCA1 repression is effective for the construction of stable and productive cell lines. Moreover, we investigated genome-wide gene expression among the CHO cell lines which have various chromosome numbers and determined the differentially-expressed genes among these cell lines and estimated the functions. Also, we determined the suitable target chromosomal region for site-specific genome integration.
動物バイオリアクターにおける糖鎖制御工学の展開とエリスロポイエチン生産への応用
2006.4 - 2008.3
科学研究費補助金 基盤研究(C)
Authorship:Principal investigator
動物遺伝学特論
2020
生物化学1
2020
Core Biochemistry
2019
生命システム工学基礎論
2019
化学基礎1
2019
生化学5
2019
医工連携セミナー
2018
化学基礎1
2018
遺伝子工学
2018
生命分子工学入門
2018
Core Biochemistry
2018
生命分子工学基礎論
2017
細胞工学
2012
遺伝子工学
2012
生物化学2
2012
生物学
2021.4 - 2021.9 (Aichi Institute of Technology)
Level:Undergraduate (liberal arts)
医用細胞工学
2013.4 - 2014.3 (Chubu University)
生物化学概論
2012.4 - 2013.3 (Aichi Institute of Technology)
応用生命科学特別講義II
2012.4 - 2013.3 (Ishikawa Prefectural University)
生物化学概論
2011.4 - 2012.3 (Aichi Institute of Technology)
医用細胞工学
2010.4 - 2011.3 (Chubu University)
生物化学概論
2010.4 - 2011.3 (Aichi Institute of Technology)
医用細胞工学
2009.4 - 2010.3 (Chubu University)
医用細胞工学
2008.4 - 2009.3 (Chubu University)