Updated on 2022/04/22

写真a

 
NISHIJIMA, Ken-ichi
 
Organization
Graduate School of Bioagricultural Sciences Avian Bioscience Research Center Professor
Graduate School
Graduate School of Engineering
Graduate School of Bioagricultural Sciences
Undergraduate School
School of Agricultural Sciences Department of Bioresource Sciences
Title
Professor
Contact information
メールアドレス

Degree 2

  1. 博士(農学) ( 1997.3   東京大学 ) 

  2. 修士(農学) ( 1992.3   東京大学 ) 

Research Interests 3

  1. Genetic engineering

  2. Animal cell technology

  3. 遺伝子改変鳥類

Research Areas 2

  1. Manufacturing Technology (Mechanical Engineering, Electrical and Electronic Engineering, Chemical Engineering) / Biofunction and bioprocess engineering  / Engineering@Process Engineering@Bioengineering

  2. Life Science / Applied molecular and cellular biology

Current Research Project and SDGs 4

  1. Regulation of immune responses by cellular lectins

  2. Production of Transgenic birds

  3. CHO細胞を用いた物質生産プロセスの効率化

  4. 遺伝子改変ニワトリを用いたワクチン及び医薬品タンパク質生産システムの開発

Research History 3

  1. Nagoya University   Professor

    2020.4

  2. Nagoya University   Associate professor

    2012.4 - 2020.3

  3. Department of Biotechnology, Nagoya University   Assistant

    1994.8

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    Country:Japan

Education 2

  1. The University of Tokyo   Graduate School, Division of Agricultural Science   Applied Biological Chemistry

    - 1992

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    Country: Japan

  2. The University of Tokyo   Faculty of Agriculture   Department of Applied Biological Chemistry

    - 1990

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    Country: Japan

Professional Memberships 8

  1. 日本動物細胞工学会   庶務幹事

    2011.6

  2. 日本生物工学会

  3. 日本農芸化学会

  4. 日本生化学会

  5. 分子生物学会

  6. 日本免疫学会

  7. 化学工学会

  8. 日本糖質学会

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Committee Memberships 1

  1. 生物遺伝資源委員会   委員  

    2020.4   

Awards 5

  1. 日本農芸化学会2018年度大会トピックス賞

    2018.3   日本農芸化学会   培養始原生殖細胞の樹立とCRISPR/Cas9によるeGFPノックインニワトリの作製

    萩原遥太、奥嵜雄也、金岡英徳、飯島信司、西島謙一

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    Award type:Award from Japanese society, conference, symposium, etc.  Country:Japan

  2. Wiley Blackwell Prize 2014

    2014  

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    Country:Japan

  3. 第17回生物工学論文賞

    2009   日本生物工学会  

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    Country:Japan

    Production of Recombinant Tumor Necrosis Factor Receptor/Fc Fusion Protein by Genetically Manipulated Chickens

  4. 第15回生物工学論文賞

    2007.9   社団法人日本生物工学会  

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    Country:Japan

  5. 日本動物細胞工学会奨励賞

    2006.9   日本動物細胞工学会  

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    Country:Japan

    動物細胞機能の有効利用に関する細胞工学的研究

 

Papers 60

  1. Siglec-9 enhances IL-10 production in macrophages via tyrosine-based motifs Reviewed

    Munetoshi Ando, Wenjie Tu, Ken-ichi Nishijima, and Shinji Iijima

    Biochem. Biophys. Res. Commun.   Vol. 369   page: 878-883   2008

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    Authorship:Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.bbrc.2008.02.111

  2. Regulatory mechanism of chicken lysozyme gene expression in oviducts examined using transgenic technology

    Kojima Yusuke, Okuzaki Yuya, Nishijima Ken-ichi, Moriwaki Shuichiro, Asai Seiya, Kaneoka Hidenori, Iijima Shinji

    JOURNAL OF BIOSCIENCE AND BIOENGINEERING   Vol. 131 ( 4 ) page: 453 - 459   2021.4

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  3. Primordial germ cell-specific expression ofeGFPin transgenic chickens Reviewed

    Hagihara Yota, Okuzaki Yuya, Matsubayashi Kazuma, Kaneoka Hidenori, Suzuki Takayuki, Iijima Shinji, Nishijima Ken-ichi

    GENESIS   Vol. 58 ( 8 )   2020.8

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    Language:Japanese   Publishing type:Research paper (scientific journal)  

    DOI: 10.1002/dvg.23388

    Web of Science

  4. Siglec-F is induced by granulocyte-macrophage colony-stimulating factor and enhances interleukin-4-induced expression of arginase-1 in mouse macrophages Reviewed

    Tateyama Hiroyuki, Murase Yusuke, Higuchi Hiroshi, Inasaka Yui, Kaneoka Hidenori, Iijima Shinji, Nishijima Ken-ichi

    IMMUNOLOGY   Vol. 158 ( 4 ) page: 340 - 352   2019.12

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    Language:Japanese   Publishing type:Research paper (scientific journal)  

    DOI: 10.1111/imm.13121

    Web of Science

  5. PRDM14 and BLIMP1 control the development of chicken primordial germ cells Reviewed

    Okuzaki Yuya, Kaneoka Hidenori, Suzuki Takayuki, Hagihara Yota, Nakayama Yuki, Murakami Seitaro, Murase Yusuke, Kuroiwa Atsushi, Iijima Shinji, Nishijima Ken-ichi

    DEVELOPMENTAL BIOLOGY   Vol. 455 ( 1 ) page: 32 - 41   2019.11

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    Language:Japanese   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.ydbio.2019.06.018

    Web of Science

  6. Identification of transgene integration site and anatomical properties of fluorescence intensity in a EGFP transgenic chicken line Reviewed

    Tsujino Kaori, Okuzaki Yuya, Hibino Nobuyuki, Kawamura Kazuki, Saito Seiji, Ozaki Yumi, Ishishita Satoshi, Kuroiwa Atsushi, Iijima Shinji, Matsuda Yoichi, Nishijima Kenichi, Suzuki Takayuki

    DEVELOPMENT GROWTH & DIFFERENTIATION   Vol. 61 ( 7-8 ) page: 393 - 401   2019.9

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1111/dgd.12631

    Web of Science

  7. Molecular cloning of chicken TET family genes and role of chicken TET1 in erythropoiesis Reviewed

    Yuya Okuzaki, Hidenori Kaneoka, Ken-ichi Nishijima, Seitaro Murakami, Yuki Ozawa, Shinji Iijima

    Biochemical and Biophysical Research Communications   Vol. 490 ( 3 ) page: 753 - 759   2017.8

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.bbrc.2017.06.113

    Web of Science

  8. Expression of interferon-inducible transmembrane proteins in the chicken and possible role in prevention of viral infections Reviewed

    Shunsuke Kidani, Yuya Okuzaki, Hidenori Kaneoka, Seiya Asai, Seitaro Murakami, Yusuke Murase, Shinji Iijima, Ken-ichi Nishijima

    Cytotechnology   Vol. 69 ( 3 ) page: 477 - 484   2017.6

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    Authorship:Last author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1007/s10616-016-9958-1

    Web of Science

  9. Characterization of chicken interferon-inducible transmembrane protein-10 Reviewed

    Yuya Okuzaki, Shunsuke Kidani, Hidenori Kaneoka, Shinji Iijima and Ken-ichi Nishijima

    Bioscience, Biotechnology and Biochemistry   Vol. 81 ( 5 ) page: 914 - 921   2017.5

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    Authorship:Last author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1080/09168451.2016.1274639

    Web of Science

  10. Constitutively expressed Siglec-9 inhibits LPS-induced CCR7, but enhances IL-4-induced CD200R expression in human macrophages Reviewed

    Hiroshi Higuchi, Toru Shoji, Shinji Iijima, Ken-ichi Nishijima

    Bioscience, Biotechnology and Biochemistry   Vol. 80 ( 6 ) page: 1141-1148   2016.6

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  11. Analyses of chicken sialyltransferases related to O-glycosylation Reviewed

    Shunsuke Kidani, Yuya Okuzaki, Hidenori Kaneoka, Seiya Asai, Seitaro Murakami, Ken-ichi Nishijima, Shinji Iijima,

    Journal of Bioscience and Bioengineering     2016.4

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.jbiosc.2016.03.017

  12. Siglec-9 modulated IL-4 responses in the macrophage cell line RAW264. Reviewed

    Higuchi H, Shoji T, Murase Y, Iijima S, Nishijima K

    Bioscience, Biotechnology and Biochemistry   Vol. 80 ( 3 ) page: 501–509   2016.3

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  13. A soluble form of Siglec-9 provides a resistance against Group B Streptococcus (GBS) infection in transgenic mice Reviewed

    Mitsumasa Saito, Sayo Yamamoto, Kinuyo Ozaki, Yukiko Tomioka, Haruka Suyama, Masami Morimatsu, Ken-ichi Nishijima, Shin-ichi Yoshida, Etsuro Ono

    Microbial Pathogenesis   Vol. 99   page: 106-110   2016

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  14. Analyses of chicken sialyltransferases related to N-glycosylation Reviewed

    Yusuke Kojima, Akifumi Mizutani, Yuya Okuzaki, Ken-ichi Nishijima, Hidenori Kaneoka, Takako Sasamoto, Katsuhide Miyake, Shinji Iijima

    Journal of Bioscience and Bioengineering   Vol. 119 ( 6 ) page: 623-628   2015.6

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    DOI: doi:10.1016/j.jbiosc.2014.11.009

  15. Lectin-dependent localization of cell surface sialic acid-binding lectin Siglec-9 Reviewed

    Munetoshi Ando, Toru Shoji, Wenjie Tu, Hiroshi Higuchi, Ken-ichi Nishijima, and Shinji Iijima

    Cytotechnology   Vol. 67   page: 601-608   2015

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    Authorship:Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1007/s10616-014-9691-6

  16. Enhanced lentiviral vector production in 293FT cells expressing Siglec-9 Reviewed

    Toru Shoji, Hiroshi Higuchi, Yoshinori Zaitsu, Ken-ichi Nishijima and Shinji Iijima

    Cytotechnology   Vol. 67   page: 593-600   2015

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    DOI: 10.1007/s10616-013-9679-7

  17. Effects of Siglec on the expression of IL-10 in the macrophage cell line RAW264 Reviewed

    Toru Shoji, Hiroshi Higuchi, Ken-ichi Nishijima and Shinji Iijima

    Cytotechnology   Vol. 67   page: 633-639   2015

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    DOI: 10.1007/s10616-014-9717-0

  18. Siglec-9 modulated IL-4 responses in the macrophage cell line RAW264 Reviewed

    Hiroshi Higuchi, Toru Shoji, Yusuke Murase, Shinji Iijima, Ken-ichi Nishijima

    Bioscience, Biotechnology and Biochemistry     page: in press   2015

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  19. A soluble form of Siglec-9 provides an antitumor benefit against mammary tumor cells expressing MUC1 in transgenic mice Reviewed

    Yukiko Tomioka, Masami Morimatsu, Ken-ichi Nishijima, Sayo Yamamoto, Haruka Suyama, Kinuyo Ozaki, Etsuro Ono

    Biochem Biophys Res Commun   Vol. 450 ( 1 ) page: 532-537   2014.6

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    DOI: 10.1016/j.bbrc.2014.06.009

  20. Recombinant proteins produced into yolk of genetically manipulated chickens are partly sialylated in N-glycan Reviewed

    Kazuhiro Yoshida, Yuya Okuzaki, Ken-ichi Nishijima, Kenji Kyogoku, Takashi Yamashita, Yoshinori Kawabe, Makoto Motono, Masamichi Kamihira, and Shinji Iijima

    Cytotechnology     2013

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    DOI: 10.1007/s10616-013-9613-z

  21. Galactosylation of human erythropoietin produced by chimeric chickens expressing galactosyltransferase Reviewed

    Yusuke Kojima, Jun Wakita, Yujin Inayoshi, Ryosuke Suzuki, Yuki Yamada, Hidenori Kaneoka, Ken-ichi Nishijima, Shinji Iijima

    Journal of Bioscience and Bioengineering     2013

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    DOI: 10.1016/j.jbiosc.2013.11.010

  22. Resistance to influenza A virus infection in transformed cell lines expressing an anti-PB2 monoclonal antibody Reviewed

    Yoshikazu Fujimoto, Kinuyo Ozaki, Masahiro Maeda, Ken-ichi Nishijima, Hiroki Takakuwa, Koichi Otsuki, Hiroshi Kida, Etsuro Ono

    Veterinary Journal   Vol. 198 ( 2 ) page: 487-493   2013

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  23. Genetic modification of a chicken expression system for the galactosylation of therapeutic proteins produced in egg white Reviewed

    Akifumi Mizutani, Hiroyuki Tsunashima, Ken-ichi Nishijima, Takako Sasamoto, Yuki Yamada, Yasuhiro Kojima, Makoto Motono, Jun Kojima, Yujin Inayoshi, Katsuhide Miyake, Enoch Y. Park and Shinji Iijima

    Transgenic Research   Vol. 21   page: 63-75   2012

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    DOI: 10.1007/s11248-011-9511-0

  24. Chicken oviduct-specific expression of transgene by a hybrid ovalbumin enhancer and the Tet expression system Reviewed

    Daisuke Kodama, Daisuke Nishimiya, Ken-ichi Nishijima, Yuki Okino, Yujin Inayoshi, Yasuhiro Kojima, Ken-ichiro Ono, Makoto Motono, Katsuhide Miyake, Yoshinori Kawabe, Kenji Kyogoku, Takashi Yamashita, Masamichi Kamihira and Shinji Iijima

    Journal of Bioscience and Bioengineering   Vol. 113   page: 146-153   2012

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  25. Moloney murine leukemia virus integrase and reverse transcriptase interact with PML proteins Reviewed

    Yuuki Okino, Yujin Inayoshi, Yusuke Kojima, Shunsuke Kidani, Hidenori Kaneoka, Akiko Honkawa, Hiroshi Higuchi, Ken-ichi Nishijima, Katsuhide Miyake, and Shinji Iijima

    Journal of Biochemistry     2012

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    DOI: 10.1093/jb/mvs063

  26. Production of Transgenic Chickens from Purified Primordial Germ Cells Infected with a Lentiviral Vector Reviewed

    Makoto Motono, Yuki Yamada, Yuki Hattori, Ryo Nakagawa, Ken-ichi Nishijima, and Shinji Iijima

    Journal of Bioscience and Bioengineering   Vol. 109 ( 4 ) page: 315-321   2010

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  27. Transcription factor YY1 interacts with retroviral integrases and facilitates integration of Moloney murine leukemia virus cDNA into the host chromosomes Reviewed

    Yujin Inayoshi, Yuuki Okino, Katsuhide Miyake, Akifumi Mizutani, Junko Yamamoto-Kishikawa, Yuya Kinoshita, Yusuke Morimoto, Kazuhito Imamura, Mahboob Morshed, Ken Kono, Toshinari Itoh, Ken-ichi Nishijima, and Shinji Iijima

    Journal of Virology   Vol. 84 ( 16 ) page: 8250-8261   2010

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  28. Production of chimeric monoclonal antibodies by genetically manipulated chickens Reviewed

    Masamichi Kamihira, Yoshinori Kawabe, Takuya Shindo, Ken-ichiro Ono, Kazuhisa Esaka, Takashi Yamashita, Ken-ichi Nishijima, and Shinji Iijima

    Journal of Biotechnology   Vol. 141   page: 18-25   2009

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  29. Production of human erythropoietin by chimeric chickens Reviewed

    Daisuke Kodama, Daisuke Nishimiya, Ken-ichi Iwata, Kazuhisa Yamaguchi, Kazuhiro Yoshida, Yoshinori Kawabe, Makoto Motono, Hiroyuki Watanabe, Takashi Yamashita, Ken-ichi Nishijima, Masamichi Kamihira, and Shinji Iijima

    Biochem. Biophys. Res. Commun.   Vol. 367   page: 834-839   2008

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  30. Retroviral gene transduction into chicken embryo gonads through blood circulation Reviewed

    Yoshinori Kawabe, Tsutomu Naka, Hiroyuki Komatsu, Ken-ichi Nishijima, Shinji Iijima, and Masamichi Kamihira

    Journal of Bioscience and Bioengineering   Vol. 106 ( 6 ) page: 598-601   2008

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  31. Analysis of chicken primordial germ cells Reviewed

    Makoto Motono, Takuya Ohashi, Ken-ichi Nishijima, and Shinji Iijima

    Cytotechnology   Vol. 57   page: 199-205   2008

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  32. Production of Recombinant TNFR/Fc Fusion Protein by Genetically Manipulated Chickens Reviewed

    Kenji Kyogoku, Kazuhiro Yoshida, Hiroyuki Watanabe, Takashi Yamashita, Yoshinori Kawabe, Makoto Motono, Ken-ichi Nishijima, Masamichi Kamihira, and Shinji Iijima

    J. Biosci. Bioeng.   Vol. 105   page: 454-459   2008

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  33. Chicken Ovalbumin Promoter is Demethylated upon Expression in the Regions Specifically Involved in Estrogen-Responsiveness Reviewed

    Mahboob Morshed, Shusuke Sano, Daisuke Nishimiya, Munetoshi Ando, Ken-ichi Nishijima, and Shinji Iijima

    Biosci. Biotech. Biochem   Vol. 70 ( 6 ) page: 1438-1446   2006

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  34. Transport of human immunoglobulin G and Fc-fusion proteins to chicken egg yolk Reviewed

    Yoshinori Kawabe, Tsutomu Naka, Naoko Ando-Noumi, Hiroyuki Matsumoto, Ken-ichiro Ono, Ken-ichi Nishijima, Masamichi Kamihira, and Shinji Iijima

    J. Biosci. Bioeng.   Vol. 102 ( 6 ) page: 518-523   2006

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  35. Production of scFv-Fc Fusion Protein by Genetically Manipulated Quails Reviewed

    Yoshinori Kawabe, Masamichi Kamihira, Ken-ichiro Ono, Kenji Kyogoku, Ken-ichi Nishijima, and Shinji Iijima

    J. Biosci. Bioeng.   Vol. 102 ( 4 ) page: 297-303   2006

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  36. YY1 binds to regulatory element of chicken lysozyme and ovalbumin promoters Reviewed

    Mahboob Morshed, Munetoshi Ando, Junko Yamamoto, Akitsu Hotta, Hidenori Kaneoka, Jun Kojima, Ken-ichi Nishijima, Masamichi Kamihira and Shinji Iijima

    Cytotechnology   Vol. 10.1007/s10616-006-9017-4   2006

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  37. Characterization of transient expression system for retroviral vector production Reviewed

    Akitsu Hotta, Yoshikazu Saito, Kenji Kyogoku, Yoshinori Kawabe, Ken-ichi Nishijima, Masamichi Kamihira, Shinji Iijima

    J. Biosci. Bioeng   Vol. 101 ( 4 ) page: 361-368   2006

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  38. High level expression of single-chain Fv-Fc fusion protein in serum and egg white of geneticallu manipulated chickens using a retroviral vector Reviewed

    Masamichi Kamihira, Ken-ichiro Ono, Kazuhisa Esaka, Ken-ichi Nishijima, Ryoko Kigaku, Hiroyuki Komatsu, Takashi Yamashita, Kenji Kyogoku and Shinji Iijima

    Journal of Virology   Vol. 79 ( 17 ) page: 10864-10874   2005

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  39. Costimulation of T cell proliferation by anti-L-selectin antibody is associated with the reduction of a cdk inhibitor p27 Reviewed

    Ken-ichi Nishijima, Munetoshi Ando, Shusuke Sano, Aiko Hayashi-Ozawa, Yoshinori Kinoshita

    Immunology   Vol. 116   page: 347-353   2005

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  40. Production of anti-CD2 chimeric antibody by recombinant animal cells Reviewed

    Akitsu Hotta, Masamichi Kamihira, Mahboob Morshed, Kanako Itoh, Yoshinori Kawabe, Ken-ichiro Ono, Hiroyuki Matsumoto, Ken-ichi Nishijima and Shinji Iijima

    J. Biosci. Bioeng.   Vol. 98 ( 4 ) page: 298-303   2004

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  41. Production of anti-prion scFv-Fc fusion proteins by recombinant animal cells Reviewed

    Kenichiro Ono, Masamichi Kamihira, Yuko Kuga, Hiroyuki Matsumoto, Akitsu Hotta, Toshinari Itoh, Ken-ichi Nishijima, Naoto Nakamura, Haruo Matsuda and Shinji Iijima

    J. Biosci. Bioeng.   Vol. 95 ( 3 ) page: 231-238   2003

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  42. Simple assay method for endocrine disrupters by in vitro quail embryo culture: nonylphenol acts as a weak estrogen in quail embryos Reviewed

    Ken-ichi Nishijima, Kazuhisa Esaka, Hisayo Ibuki, Kenichiro Ono, Katsuhide Miyake, Masamichi Kamihira and Shinji Iijima

    J. Biosci. Bioeng.   Vol. 95 ( 6 ) page: 612-617   2003

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  43. Production of transgenic quails with high frequency of germ-line transmission using VSV-G pseudotyped retroviral vector Reviewed

    Shinji Mizuarai, Kenichiro Ono, Kazuhisa Yamaguchi, Ken-ichi Nishijima, Masamichi Kamihira and Shinji Iijima

    Biochem. Biophys. Res. Commun.   Vol. 286 ( 3 ) page: 456-463   2001

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  44. The effects of cell adhesion on the growth and protein productivity of animal cells Reviewed

    Ken-ichi Nishijima, Tsukasa Fujiki, Hirotoshi Kojima and Shinji Iijima

    Cytotechnology   Vol. 33   page: 147-155   2000

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  45. Integrase-mediated nonviral gene transfection with enhanced integration efficiency Reviewed

    Journal of Bioscience and Bioengineering   Vol. 88 ( 5 ) page: 461-467   1999

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  46. Amplification of competitive telomere sequence in living animal cells induces chromatin instability Reviewed

    Shinji Mizuarai, Ken-ichi Nishijima and Shinji Iijima

    Cytotechnology   Vol. 31   page: 193-201   1999

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  47. Induction of oral tolerance in splenocyte-reconstituted SCID mice Reviewed

    Hiroko Yoshida, Satoshi Hachimura, Kazuki Hirahara, Tatsuhiro Hisatsune, Ken-ichi Nishijima, Akio Shiraishi and Shuichi Kaminogawa

    Clinical Immunology and Immunopathology   Vol. 87 ( 3 ) page: 282-291   1998

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  48. Oral administration of antigen does not influence the proliferation and IFN-α production of responsive CD8+ T cells but enables to establish T cell clones with different lymphokine production profile Reviewed

    Ken-ichi Nishijima, Tatsuhiro Hisatsune, Hiroko Kato, Masako Kohyama, Masahiro Kakehi, Satoshi Hachimura and Shuichi Kaminogawa

    Cytotechnology   Vol. 25   page: 89-100   1997

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  49. Enhancing effect of interleukin-4 on the secretion of interferon-γ by αs1-casein-specific CD8+ T cells. Reviewed

    Ken-ichi Nishijima, Masako Kohyama, Tatsuhiro Hisatsune, Hiroko Kato, Masahiro Kakehi and Shuichi Kaminogawa

    Bioscience, Biotechnology and Biochemistry   Vol. 61 ( 7 ) page: 1156   1997

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  50. 食餌抗原に応答して抑制性リンフォカインを産生するCD8+T細胞 Invited

    西島謙一、上野川修一

    医学の歩み   Vol. 177 ( 5 ) page: 364-367   1996

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  51. Difference in signal transduction for IL-10 and IFN-r production in a DC8+ T cell clone. Reviewed

    Yuji Minai, Masao Goto, Masako Kohyama, Tatsuhiro Hisatsune, Ken-ichi Nishijima and Shuichi Kaminogawa

    Cellular Immunology   Vol. 172 ( 2 ) page: 200   1996

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  52. CD8+ T cells specific to the exogenous antigen. Mode of antigen recognition and possible implicationin immunosuppression Reviewed

    Tatsuhiro Hisatsune, Ken-ichi Nishijima, Masako Kohyama, Hiroko Kato and Shuichi Kaminogawa

    The Journal ofImmunology   Vol. 154   page: 88   1995

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  53. αS1-casein-specific CD8+ T cell clone that inhibits its own interferon-gamma production by producing interleukin-10 Reviewed

    Ken-ichi Nishijima, Tatsuhiro Hisatsune, Hiroko Kato, Osamu Shiho and Shuichi Kaminogawa

    Bioscience, Biotechnology, and Biochemistry   Vol. 59 ( 12 ) page: 2274-2276   1995

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  54. CD4+ T cells anergized by high-dose feeding establish oral tolerance to antibody responses when transfected in SCID and nude mice Reviewed

    Kazuki Hirahara, Tatsuhiro Hisatsune, Ken-ichi Nishijima, Hiroko Kato, Osamu Shiho and Shuichi Kaminogawa

    Journal of Immunology   Vol. 154   page: 6238-6245   1995

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  55. Anti-IL-10 antibodyenhances the proliferation of CD8+ Tcell clones : autoregulatory role of murineIL-10 in CD8+ T cells Reviewed

    Ken-ichi Nishijima, Tatsuhiro Hisatsune, Yuji Minai, Masako Kohyama and Shuichi Kaminogawa

    Cellular Immunology   Vol. 154   page: 193   1994

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

  56. Activation via TCR of IL-2 receptor of a CD8+ suppressor T cell clone: Effect on IL-10 production and on proliferation of suppressor T cell Reviewed

    Yuji Minai, Tatsuhiro Hisatsune, Ken-ichi Nishijima, Atsushi Enomoto and Shuichi Kaminogawa

    Cytotechnology   Vol. 14   page: 81-87   1994

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  57. Autoreactive CD8+ T cell clones producing immunesuppressive lymphokines IL-10 andinterferon-γ Reviewed

    Tatsuhiro Hisatsune, Ken-ichi Nishijima, Yuji Minai, Masako Kohyama and Shuichi Kaminogawa

    Cellular Immunology   Vol. 154   page: 181   1994

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  58. A suppressive lymphokine derived from Ts clone 13G2 is IL-10 Reviewed

    Tatsuhiro Hisatsune, Yuji Minai, Ken-ichi Nishijima, Atsushi Enomoto, Kevin W. Moore, Takashi Yokota, Ken-ichi Arai and Shuichi Kaminogawa

    Lymphokine and Cytokine Research   Vol. 11   page: 87   1992

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  59. Strong influence ofthe processing of the antigen on negativeeffects on T cell activation by regions outsidethe determinant area of bovine αS1-casein Reviewed

    Tatsuhiro Hisatsune, Akio Ametani, Ken-ichi Nishijima, Atsushi Enomoto and Shuichi Kaminogawa

    Bioscience, Biotechnology Biochemistry   Vol. 56   page: 1616   1992

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  60. CD8+ suppressorT cell clone capable of inhibiting the antigen- and anti-T cell receptor-induced proliferation of Th clones without cytolytic activity Reviewed

    Tatsuhiro Hisatsune, Atushi Enomoto, Ken-ichi Nishijima, Yuji Minai, Yoshihiro Asano, Tomio Tada and Shuichi Kaminogawa,

    The Journal ofImmunology   Vol. 145   page: 2421   1990

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Books 25

  1. Transgenic chickens Reviewed

    Ken-ichi Nishijima and Shinji Iijima( Role: Joint author)

    Development, Growth & Differentiation   2013 

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    Language:English Book type:Textbook, survey, introduction

  2. シグレックと免疫制御 Reviewed

    西島謙一( Role: Contributor)

    糖鎖生物学. 北島健、佐藤ちひろ、門松健治、加藤晃一編  名古屋大学出版会  2020.2 

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    Total pages:297   Responsible for pages:237-238   Language:Japanese Book type:Textbook, survey, introduction

  3. 糖鎖から見たインフルエンザの適応変異 Reviewed

    西島謙一( Role: Contributor)

    化学と生物  2019.3 

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    Language:Japanese

  4. 動物細胞がin vitro培養可能になったころ Reviewed

    西島謙一( Role: Sole author ,  続・生物工学基礎講座 バイオよもやま話)

    日本生物工学会誌  2016.12 

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    Language:Japanese Book type:Textbook, survey, introduction

  5. シアル酸による生体機能調節

    西島謙一( Role: Sole author)

    JAACT Newsletter   2014.4 

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    Language:Japanese Book type:Textbook, survey, introduction

  6. タンパク質の次は糖鎖の時代になるの? Reviewed

    西島謙一( Role: Sole author)

    化学同人   2012.10 

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    Language:Japanese Book type:Textbook, survey, introduction

  7. 遺伝子組換え鳥類の作製と抗体生産技術 Reviewed

    西島謙一( Role: Sole author)

    CMC出版 「バイオ医薬品製造の効率化と生産基材の開発」  2012.4 

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    Language:Japanese Book type:Textbook, survey, introduction

  8. ニワトリ卵白に生産させた医薬用タンパク質の糖鎖制御 Reviewed

    西島謙一( Role: Sole author)

    バイオインダストリー協会  2011 

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  9. レトロウイルスベクターによる遺伝子導入JAACT Newsletter

    西島謙一( Role: Sole author)

    日本動物細胞工学会  2010 

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  10. 遺伝子導入鳥類の卵中への医薬品糖タンパク質生産 Reviewed

    西島謙一( Role: Sole author)

    学会出版センター  2009 

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    化学と生物47, 163-168

  11. Sterilization of chicken primordial germ cells

    Makoto Motono, Hiroyuki Komatsu, Yoshinori Kawabe, Ken-ichi Nishijima, and Shinji Iijima( Role: Joint author)

    Springer Publishers  2008 

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    Language:English

    Animal Cell Technology: Basic & Applied Aspects vol. 15

  12. Immune regulation by sialoglycoconjugate-binding lectins t cell costimulation by L-selectin

    Munetoshi Ando, Ken-ichi Nishijima, Shusuke Sano, Aiko Hayashi, Yoshinori Kinoshita, and Shinji Iijima( Role: Joint author)

    Springer Publishers  2005 

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    Animal Cell Technology: Basic & Applied Aspects vol. 14", (S. Iijima and K. Nishijima, Eds.)

  13. Production of chimeric antibodies by transgenic chicken bioreactors

    Yoshinori Kawabe, Akitsu Hotta, Ken-Ichiro Ono, Kazuhisa Esaka, Ken-Ichi Nishijima, Masamichi Kamihira, and Shinji Iijima( Role: Joint author)

    Springer Publishers  2005 

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    Animal Cell Technology: Basic & Applied Aspects vol. 14", (S. Iijima and K. Nishijima, Eds.)

  14. Biochemical analysis of chicken ovalbumin promoter

    Mahboob Morshed, Junko Yamamoto, Shusuke Sano, Ken-Ichi Nishijima, Masamichi Kamihira, and Shinji Iijima( Role: Joint author)

    Springer Publishers  2005 

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    Animal Cell Technology: Basic & Applied Aspects vol. 14", (S. Iijima and K. Nishijima, Eds.)

  15. Preparation of high-titer retroviral vectors using transient expression system

    Akitsu Hotta, Yoshikazu Saito, Ken-ichi Nishijima, Msamichi Kamihira, and Shinji Iijima( Role: Joint author)

    Springer Publishers  2005 

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    Animal Cell Technology: Basic & Applied Aspects vol. 14", (S. Iijima and K. Nishijima, Eds.)

  16. "Production of pharmaceutical proteins by transgenic avian", in "Animal Cell Technology: Basic & Applied Aspects vol. 13" (Yagasaki, K. et al., Eds.)

    Akitsu Hotta, Ken-ichiro Ono, Shinji Mizuarai, Kazuhisa Yamaguchi, Ken-ichi Nishijima, Masamichi Kamihira and Shinji Iijima,( Role: Joint author)

    Kluwer Academic Publishers  2004 

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    Language:English

  17. "Transgenic birds for the production of recombinant proteins" in Adv Biochem Engin/Biotechnol

    Masamichi Kamihira, Ken-ichi Nishijima, Shinji Iijima( Role: Joint author)

    Springer-Verlag  2004 

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    Language:English

  18. ”金の卵を産むニワトリをつくる トランスジェニック鳥類を使った有用タンパク質の生産”、化学と生物 Reviewed

    上平正道、西島謙一、飯島信司( Role: Joint author)

    学会出版センター  2003 

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  19.  ”サイトカイン”、’食品と体 免疫・アレルギーのしくみ'(上野川修一編集) Reviewed

    西島謙一( Role: Joint author)

    朝倉書店  2003 

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  20. ”敵か味方か?:拒絶と受容を分ける免疫系の鍵分子”、生物工学会誌 Reviewed

    西島謙一( Role: Sole author)

    日本生物工学会  2003 

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  21. ”動物培養細胞”、’生物化学実験法45 組換えタンパク質生産法(塚越規弘編著)’ Reviewed

    飯島信司、上平正道、西島謙一( Role: Joint author)

    学会出版センター  2001 

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  22. Effect of cell adhesion on the growth of animal cells

    Hirotoshi Kojima, Ken-ichi Nishijima and Shinji Iijima( Role: Joint author)

    Recent Developments in Biochemical Engineering  1998 

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  23. Suppressive lympholines produced by CD8+ T cells

    ( Role: Joint author)

    Animal Cell Technology: Basic & Applied Aspects.  1994 

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  24. Mechanism of IL-10 poroduction by a CD8+ T cell clone

    Yuji Minai, Tatsuhiro Hisatsune, Ken-ichi Nishijima, Atsushi Enomoto and Shuichi Kaminogawa( Role: Joint author)

    Animal Cell Technology: Basic & Applied AspectsKluwer Academic Publishers, Netherlands  1993 

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  25. CD8+ suppressor T cell clone 13G2 secretes a suppressive lymphokine, immune suppressive factor-T (ISF-T)

    ( Role: Joint author)

    Animal Cell Culture and Production of Biologicals  1991 

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Presentations 6

  1. Chicken/Quail resources in Avian Bioscience Research Center in Nagoya University Invited International conference

    K. Nishijima

    Animal Genomics and Genome Project  2021.11.25 

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    Event date: 2021.11

    Language:English   Presentation type:Public lecture, seminar, tutorial, course, or other speech  

  2. ニワトリ始原生殖細胞の 遺伝子改変への応用 Invited

    西島謙一

    基盤技術整備プログラム成果発表ワークショップ 

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    Event date: 2021.8

    Language:Japanese   Presentation type:Symposium, workshop panel (nominated)  

  3. Genetically Manipulated Chickens as Animal Bioreactor. Invited International conference

    Nishijima, K.; Kaneoka, H.; Iijima, S

    2016 World Congress on In Vitro Biology 

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    Event date: 2016.6

    Language:English   Presentation type:Oral presentation (general)  

    Venue:San Diego   Country:United States  

  4. Toward the understanding of transgenesis property of chicken PGCs International conference

    Ken-ichi Nishijima

    International Forum on Avian Germplasm 2013 

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    Event date: 2013.10

    Language:English   Presentation type:Oral presentation (general)  

    Country:Korea, Republic of  

  5. Siglec-9 inhibits innate immunity International conference

    Sialoglycoscience 2006, Fifth International Conference 

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    Event date: 2006

    Language:English   Presentation type:Poster presentation  

    Country:Japan  

  6. Production of Antibody by Transgenic Chicken. International conference

    2004 World Congress on In Vitro Biology 

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    Event date: 2004.5

    Language:English   Presentation type:Oral presentation (invited, special)  

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Research Project for Joint Research, Competitive Funding, etc. 9

  1. 遺伝子改変鶏を利用した新規ワクチン生産プラットフォーム

    2021.4 - 2024.3

    善き未来を拓く科学技術 

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    Authorship:Principal investigator  Grant type:Competitive

    Direct Cost: \25000000 )

  2. ニワトリ糖鎖制御技術の開発と炎症調節への展開

    2018.4 - 2019.3

    水谷糖質科学振興財団   水谷糖質科学振興財団 第25回研究助成 

    西島謙一

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    Authorship:Principal investigator  Grant type:Competitive

    Direct Cost: \3000000 )

  3. 新規高機能インフルエンザワクチン生産法の確立

    2012.4 - 2013.3

    公益財団法人豊田理化学研究所 平成24年度豊田理研スカラー 

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    Grant type:Competitive

  4. 炎症を制御するシアル酸認識レクチンの機能解析

    2011.12 - 2012.12

    財団法人テルモ科学技術振興財団 2011年度一般研究助成 

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    Grant type:Competitive

  5. トランスジェニックニワトリを用いた高機能インフルエンザワクチン生産法

    2011.12 - 2012.3

    財団法人中部科学技術センター 学術奨励研究助成金 

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    Grant type:Competitive

  6. シアル酸結合レクチンによる食餌性脂質作用の調節

    2009

    三島海雲記念財団 

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    Grant type:Competitive

  7. トランスジェニック鳥類による糖鎖改変タンパク質の生産

    2007

    財団法人旭硝子財団平成19年度採択研究助成 

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    Grant type:Competitive

  8. 細胞表面シアル酸制御を介した自然免疫調節

    2007

    財団法人武田科学振興財団2007年度一般研究奨励 

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    Grant type:Competitive

  9. 遺伝子導入鳥類の卵中への有用蛋白質生産技術

    2002.10 - 2005.9

    国内共同研究 

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    トランスジェニック鳥類を用いたタンパク質性生理活性物質の大量生産法を技術開発を目指した。その結果、モデルタンパク質で卵一個あたり最大200mgもの高い生産性を得ることが出来た。

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KAKENHI (Grants-in-Aid for Scientific Research) 8

  1. デザイン型育種プロセスの創成とインフルエンザ抵抗性ニワトリ作製への展開

    Grant number:21K18186  2021.7 - 2024.3

    科学研究費助成事業  挑戦的研究(開拓)

    西島 謙一, 西藤 岳彦, 内田 裕子, 佐久間 咲希

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    Authorship:Principal investigator 

    Grant amount:\26000000 ( Direct Cost: \20000000 、 Indirect Cost:\6000000 )

    鳥インフルエンザは世界中に蔓延している。特に、高病原性鳥インフルエンザは高い致死性と強い伝播性から、発生農場の全家禽の殺処分等の防疫措置をとる必要がある。本研究では、鶏が本来持つインフルエンザ耐性遺伝子を新規に同定することを目指す。最終的に、現在の商用鶏の高病原性トリインフルエンザに対する高い感受性を改善した新たな鶏系統を作出することを目指す。発見したインフルエンザ耐性遺伝子をコマーシャル系統に導入することができれば、鳥インフルエンザ蔓延防止と食糧の安定供給に大きく貢献できる。また、鳥インフルエンザは変異ウイルスがヒトのパンデミックを起こしてきた歴史があり、公衆防疫上も波及効果は絶大である。

  2. Development of integrated animal cell engineering system for biopharmaceutical production

    Grant number:20H00322  2020.4 - 2024.3

    Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (A)

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    Authorship:Coinvestigator(s) 

  3. ゲノム編集ニワトリによる高機能インフルエンザワクチンの生産

    Grant number:20H02537  2020.4 - 2024.3

    科学研究費助成事業  基盤研究(B)

    西島 謙一, 小野 悦郎, 金岡 英徳

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    Authorship:Principal investigator 

    Grant amount:\17680000 ( Direct Cost: \13600000 、 Indirect Cost:\4080000 )

    世界的流行の恐れから、インフルエンザワクチン生産法の抜本的改善が急がれている。発育鶏卵による従来法はワクチン生産に適した自動化機器類などが整備されており、今後もワクチンの大部分はこの方法により生産されるものと考えられる。本申請では、これまで開発してきた家禽の遺伝子操作技術を用いて新たなトランスジェニックニワトリを作製し、高力価なヒトインフルエンザワクチンを生産する新たな手法を提供することを目指す。本申請ではこのための基盤技術として精子を持たないニワトリ系統の作製も行う。ニワトリはワクチン生産だけでなく最も身近な家畜でもあり、本研究が完成されれば社会生活に対する影響は大きい。
    発育鶏卵でインフルエンザウイルスの増殖を左右するのは漿尿膜上に存在する糖鎖の結合パターンであり、理論上ニワトリ型の糖鎖をヒト型化できれば、ヒトウイルスを馴化プロセスなしで増やせるようになる。そこで、本申請ではニワトリの遺伝子改変技術を用いてヒト型糖鎖発現ニワトリを作製することを目指す。安全性を確保するために2系統の遺伝子改変ニワトリを作成する計画であるが、本年度はこの2系統の遺伝子改変始原生殖細胞株を作製しレシピエント胚に移植することで生殖腺キメラを作製した。一部は成熟したため、遺伝子改変された次世代のニワトリを得るために交配とスクリーニングを始めている。この過程で、シアル酸転移酵素の発現がニワトリ細胞の増殖にマイナスに作用することを見いだした。増殖抑制の強度はそれほど大きくないため、発現により発生に悪影響を及ぼす可能性は低いものと考えられたが、この点についてはさらに検討を行う必要がある。組織特異的な発現が必要になる可能性もあり、そのためのモデルとしてリゾチームプロモーターの解析を行った。
    また、我々が着目してきたPRDM14遺伝子のニワトリ始原生殖細胞における役割を確認するために、PRDM14を誘導発現可能なコンストラクトを導入する一方、内在性のPRDM14遺伝子を両アレルとも破壊した始原生殖細胞株を作製した。PRDM14の発現量は期待通り制御できたが、調べた範囲で始原生殖細胞の増殖及び遺伝子発現に影響はなかった。実験システムの特性により低レベルのバックグラウンド発現が残るが、この発現により細胞の性質が維持されたものと推定された。
    生殖腺キメラについては概ね計画通り作製できている。新所属における遺伝子改変ニワトリの作製作業が軌道に乗りつつあり、交配個体数をさらに増やすことで計画した系統作製は可能であると考えられる。
    引き続き生殖腺キメラを作製し交配を精力的に進める予定である。また、始原生殖細胞の維持に必要な遺伝子の解析については対象を広げBLIMP1など他の遺伝子についても検討を行う。

  4. Integrated platform for mammalian cell-based cell and bioprocess engineering

    Grant number:17H06157  2017.5 - 2022.3

    Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (S)

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    Authorship:Coinvestigator(s) 

  5. Avian transgenic technology against influenza

    Grant number:16H04574  2016.4 - 2020.3

    Nishijima Ken-ichi

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    Authorship:Principal investigator 

    Grant amount:\17550000 ( Direct Cost: \13500000 、 Indirect Cost:\4050000 )

    It was shown that chicken IFITM10 slightly reduced the infectivity of VSV-G-pseudotyped virus. In addition, overexpression of Muc13, of which gene size is below the limit to construct an expression vector, did not prevent infectivity of VSV-G-pseudotyped virus. Thus, expression system for large size mucin is under construction.
    We examined the gene transfer and genome editing conditions suitable for the long-term cultured primordial germ cells. Using CRISPR/Cas9, we succeeded in producing a genome-edited chicken in which the EGFP gene was knocked in.

  6. トランスジェニック技術を用いたニワトリ始原生殖細胞形成機構の解析

    Grant number:16H01253  2016.4 - 2018.3

    新学術領域研究(研究領域提案型)

    西島 謙一

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    Authorship:Principal investigator 

    Grant amount:\9880000 ( Direct Cost: \7600000 、 Indirect Cost:\2280000 )

    マウスにおいて始原生殖細胞分化のマスターレギュレーターとされるPrdm14に着目している。ニワトリ培養始原生殖細胞にPrdm14のsiRNAをエレクトロポレーションにより導入したところ、CvhやDazlの発現には影響を与えず、NANOGの発現が若干低下することが認められた。昨年度検討した胚盤葉細胞の過剰発現系とは異なる結果であった。この原因については現在のところ不明であるが、細胞の種類や分化段階などの違いが影響する可能性を考えている。
    FGFから活性化されるMEK/MAPKシグナルを阻害したところ、Prdm14の発現が大きく低下することが示された。一方、Activin下流のSMADの阻害剤添加ではPrdm14の発現低下は12時間よりも遅くゆっくりと起こることが示された。これらのことから、培養始原生殖細胞ではMEK/ERK経路がPrdm14の発現に重要であることが示唆された。
    一方、CRISPR/Cas9によるゲノム編集を試みたところ、培養始原生殖細胞にeGFP遺伝子をノックインすることに成功した。この細胞をレシピエント胚に移植したところ、高効率で生殖腺に定着することが確認された。成熟したオス個体ではeGFPノックイン細胞由来精子が多く存在し、野生型メスとの交配によりeGFPを持つ子孫を得ることに成功した。今後、このニワトリの解析を進める予定である。
    DNA脱メチル化に関わるニワトリTET1を解析した。酵素活性は認められたものの、PGCでの発現は低く、ほ乳類と異なりPGCの初期分化過程においてDNAメチル化に大きな変化はないものと考えられた。一方、胚赤血球においてTET1が高発現していることが示され、赤血球分化に脱メチル化が関わっていることが示唆された。
    29年度が最終年度であるため、記入しない。
    29年度が最終年度であるため、記入しない。

  7. Fundamental research for genome-wide platform of industrial mammalian cells

    Grant number:26249125  2014.4 - 2018.3

    Omasa Takeshi

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    Authorship:Coinvestigator(s) 

    The fundamental research of chromosome stability and selection of targeting region of genome, and analysis of signaling pathway for Chinese hamster ovary cells are necessary for genome-wide platform of industrial mammalian cells. We focused on the chromosome stability and find that BRCA1 repression is effective for the construction of stable and productive cell lines. Moreover, we investigated genome-wide gene expression among the CHO cell lines which have various chromosome numbers and determined the differentially-expressed genes among these cell lines and estimated the functions. Also, we determined the suitable target chromosomal region for site-specific genome integration.

  8. 動物バイオリアクターにおける糖鎖制御工学の展開とエリスロポイエチン生産への応用

    2006.4 - 2008.3

    科学研究費補助金  基盤研究(C)

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    Authorship:Principal investigator 

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Teaching Experience (On-campus) 15

  1. 動物遺伝学特論

    2020

  2. 生物化学1

    2020

  3. Core Biochemistry

    2019

  4. 生命システム工学基礎論

    2019

  5. 化学基礎1

    2019

  6. 生化学5

    2019

  7. 医工連携セミナー

    2018

  8. 化学基礎1

    2018

  9. 遺伝子工学

    2018

  10. 生命分子工学入門

    2018

  11. Core Biochemistry

    2018

  12. 生命分子工学基礎論

    2017

  13. 細胞工学

    2012

  14. 遺伝子工学

    2012

  15. 生物化学2

    2012

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Teaching Experience (Off-campus) 9

  1. 生物学

    2021.4 - 2021.9 Aichi Institute of Technology)

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    Level:Undergraduate (liberal arts) 

  2. 医用細胞工学

    2013.4 - 2014.3 Chubu University)

  3. 生物化学概論

    2012.4 - 2013.3 Aichi Institute of Technology)

  4. 応用生命科学特別講義II

    2012.4 - 2013.3 Ishikawa Prefectural University)

  5. 生物化学概論

    2011.4 - 2012.3 Aichi Institute of Technology)

  6. 医用細胞工学

    2010.4 - 2011.3 Chubu University)

  7. 生物化学概論

    2010.4 - 2011.3 Aichi Institute of Technology)

  8. 医用細胞工学

    2009.4 - 2010.3 Chubu University)

  9. 医用細胞工学

    2008.4 - 2009.3 Chubu University)

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