Faculty Profiles - HONDA, Hiroyuki

Papers - HONDA, Hiroyuki

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High celldensity culture of Rhodococcus erythropolis KA2-5-1 for in situ induction of dibenzothiophene degrading activity Reviewed

Hibiki Matsushita, Takahiro Sugiyama, Hiroyuki Honda and Takeshi Kobayashi

Journal of the Chinese Institute of Chemical Engineers Vol. 32 ( 3 ) page： 219-225 2001

Language：English Publishing type：Research paper (scientific journal)

To obtain cells with high dibenzothiophene (DBT) degrading activity, two phase fed-batch culture of Rhodococcus erythropolis KA 2-5-1 was carried out, which consisted of growth phase in the medium containing sulfate ion as a sulfur source and induction phase for expression of DBT degrading enzyme by DBT addition. Cell concentration of 24.3 g-dry cells/L was obtained after 30 h culture and the maximum specific degrading activity of 18.2 mmol/kg-dry cells/h was achieved at 10 h after induction. In order to simplify the system and lower an operational cost, next, in situ induction of the enzyme activity was conducted by means of sulfur limitation since the activity of the strain can only be induced under sulfur starvation. The strain was cultivated in fed-batch mode in the modified A medium containing 96 mg/L of sulfate ion. At 22 h, sulfate concentration decreased below 10 mg/L and approximately 10 g/L of cell concentration was obtained. When 125 mg/L of DBT was added at 22 h, the enzyme activity increased rapidly and the maximum specific activity of 14.3 mmol/kg-dry cells/h was obtained at 28 h. However, the DBT degrading activity decreased rapidly thereafter. From the assay of remaining concentration of medium components in the culture broth, it was found that Fe and Mg ions were exhausted at the end of cultivation. Fed-batch culture with feeding of concentrated solution of Fe and Mg ions was then conducted. After 23 h, 125 mg/L of DBT was added, and the enzyme activity increased rapidly. In this case, the activity did not decrease, and the maximum specific degrading activity of 18.5 mmol/kg-dry cells/h and cell concentration of 41.7 g-dry cells/L were obtained at 34 h.

Determination of operating conditions in activated sludge process using fuzzy neural network and genetic algorithm Reviewed

Hiroyuki Yoshikawa, Taizo Hanai, Shuta Tomida, Hiroyuki Honda, and Takeshi Kobayashi

Journal of Chemical Engineering of Japan Vol. 34 ( 8 ) page： 1033-1039 2001

Fussy neural network model for assessment of Alzheimer-type dementia Reviewed

Shin Hibino, Taizo Hanai, Erika Nagata, Michitaka Matsubara, Kazutoshi Fukagawa, Tatsuaki Shirataki, Hiroyuki Honda, and Takeshi Kobayashi

Journal of Chemical Engineering of Japan Vol. 34 ( 7 ) page： 936-942 2001

Enhanced and prolonged production of regenerated plantlet from carrot callus in viscous additive-supplemented medium Reviewed

Eiji Nagamori, Mariko Omote, Hiroyuki Honda and Takeshi Kobayashi

Journal of Bioscience and Bioengineering Vol. 91 ( 3 ) page： 283-287 2001

Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)

To reduce the hydrodynamic stress in plant cell culture and enhance the production of regenerated plantlets, a liquid medium containing a viscous additive was newly designed and plantlet production from embryogenic carrot callus cultivated in the medium was examined. Na-Alginate or carboxymethyl cellulose (CMC) was used as the viscous additive. The viscosity of the medium increased with increasing additive content and the number of regenerated plantlets also increased. When carrot calli were cultivated in the medium containing 0.4% CMC, designated as N medium (viscosity, 3 mPa・s), the maximum enhancement of plantlet regeneration, approximately 2.5 times higher than that in the control medium, was obtained. Enlargement of callus size observed in N medium is considered to be the main reason for the enhanced plantlet regeneration. Regeneration enhancement was sufficiently induced after calli were cultivated once in N medium, but this regenerarion ability rapidly disappeared after once cultivation in the conventional medium. In repeated batch culture using N medium, plantlet production continued at a high level for 18 batches (250 d) with no significant decrease, while in the control culture without CMC the number of plantlets produced dropped to almost zero by the sixth batch (84 d).

Lipase production in two step fed-batch culture of an organic solvent-tolerant Pseudomonas aeruginosa LST-03 Reviewed

Takeshi Ito, Hirokazu Kikuta, Eiji Nagamori, Hiroyuki Honda, Hiroyasu Ogino, Haruo Ishikawa and Takeshi Kobayashi

Journal of Bioscience and Bioengineering Vol. 91 ( 3 ) page： 245-250 2001

Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)

Efficient lipase production by two-step fed-batch culture of an organic solvent-tolerant bacterium, Pseudomonas aeruginosa LST-03, was investigated. When FB synthetic medium was used in flask culture, no lipase activity was detected, whereas lipase was produced at 2.3 I.U./ml in C2 complex medium. However, lipase production was induced in FB medium when a fatty acid was added to the culture broth in the stationary phase. Among fatty acids tested, long chain saturated fatty acids, such as C18 (stearic acid) and C20 (arachidic acid), were found to function as effective inducers for the production of lipase, giving an activity level almost the same as that obtained in C2 medium in flask culture. Two-step lipase production, comprised of a growth phase in fed-batch mode and a production phase in which lipase was induced by the addition of 5 %(v/v) stearic acid, was carried out in a jar-fermentor. In the growth phase, the maximum cell concentration at 16 h was only 20 in terms of the optical density at 660nm (OD660), and a low level of lipase production (8 I.U./ml) was obtained after 167 h. This was considered to be due to the exhaustion of several medium components brought about by the use of an unsuitable medium or feeding solution. After analyzing the contents of the compounds in the culture broth by inductively coupled plasma spectrometry for metal ions and HPLC for anions, a modified FB medium was designed. When this modified FB medium was used in two-step fed-batch culture, the maximum cell concentration reached an OD660 of 55 (30.2 g-dry cells/l) at 16.5 h, and lipase was produced at 96 I.U./ml after 35 h, which is approximately 40 times higher than the production level obtained in flask culture using C2 medium.

Fuzzy neural network-based prediction of the motif for MHC class II binding peptides Reviewed

Hideki Noguchi, Taizo Hanai, Hiroyuki Honda, Leonard C. Harrison and Takeshi Kobayashi

Journal of Biosience and Bioengineering Vol. 92 ( 3 ) page： 227-231 2001

Optimization of bovine cathepsin C production by cultivation of recombinant methylotrophic yeast Candida oidinii

Hiroyuki Kikuta, Kenjiro Kano, Hiroyuki Honda and Takeshi Kobayashi

Jornal of hemical Engineering of Japan Vol. 34 ( 6 ) page： 848-851 2001

Augmentation of MHC class I antigen presentation via heat shock protein expression by hyperthermia Reviewed

Akira Ito, Masashige Shinkai, Hiroyuki Honda, Toshihiko Wakabayashi, Jun Yoshida and Takeshi Kobayashi

Cancer Immmunology and Immunotherapy Vol. 50 page： 515-522 2001

知的推論モデルによる品質の定量化と培養プロセスの管理

本多裕之

生物工学会誌 Vol. 78 ( 3 ) page： 94-100 2000

Fuzzy control of bioprocess in Japan

Biotechnology Annual Review Vol. 5 page： 1-23 2000

Development of chitosan-magnetite aggregates containing Nitrosomonas europaea cells for nitrification enhancement

Journal of Bioscience and Bioengineering Vol. 89 ( 5 ) page： 420-425 2000

Selection of embryogenic sugarcane callus by image analysis

Journal of Bioscience and Bioengineering Vol. 87 ( 5 ) page： 700-702 1999

Recovery of recombinant Escherichia coli by chitosan-conjugated magnetite

Biochemical Engineering Journal Vol. 3 page： 157-160 1999

Model construction for quality of beer and brewing process using FNN

Kagaku Kogaku Ronbunshu Vol. 25 ( 5 ) page： 695-701 1999

Development of novel magnetic sensing for brain lesion using functional magnetic particles Reviewed

Masashige Shinkai, Akira Ohshima, Mitsugu Yanase, Tsuyoshi Uchiyama, Kaneo Mohri, Toshihiko Wakabayashi, Jun Yoshida, Hiroyuki Honda and Takeshi Kobayashi

Kagaku Kougaku Ronbunshu Vol. 24 ( 2 ) page： 174-178 1998

Temperature control of Ginjo sake mashing process by automatic fuzzy modeling using fuzzy networks Reviewed

Hiroyuki Honda, Taizo Hanai, Akemi Katayama, Hisao Tohyama and Takeshi Kobayashi

Journal of Fermentation and Bioengineering Vol. 85 ( 1 ) page： 107-112 1998

High cell density culture of Rhodococcus rhodochrous by pH-stat feeding and dibenzothiophene degradation Reviewed

Hiroyuki Honda, Hiroyasu Sugiyama, Ikuo Saito and Takeshi Kobayashi

Journal of Fermentation and Bioengineering Vol. 85 ( 3 ) page： 334-338 1998

Colominic acid production from Escherichia coli in a fed-batch culture under the control of ammonium ions using an FIA system

Journal of Fermentation and Bioengineering. Vol. 83 ( 1 ) page： 59-63 1997

Production of Fv fragment of monoclonal antibody from recombinant methylotrophic yeast, Pichia pastoris

Journal of Chemical Engineering of Japan Vol. 29 ( 2 ) page： 390-392 1996

Characteristics on heat sterilization of a novel autoclave with a turntable for rotation

Journal of Fermentation and Bioengineering Vol. 81 ( 6 ) page： 570-572 1996

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Papers - HONDA, Hiroyuki