Updated on 2022/08/09

写真a

 
KURIHARA Daisuke
 
Organization
Institute of Transformative Bio-Molecules Designated associate professor
Title
Designated associate professor

Degree 1

  1. 博士(工学) ( 2009.3   大阪大学 ) 

Research Interests 6

  1. 植物胚発生

  2. 細胞分裂

  3. 細胞運命決定

  4. ライブイメージング

  5. 二光子顕微鏡

  6. 光顕微操作

Research Areas 3

  1. Life Science / Morphology and anatomical structure

  2. Life Science / Plant molecular biology and physiology

  3. Life Science / Cell biology

Current Research Project and SDGs 1

  1. 植物における細胞運命制御機構の解明

Research History 10

  1. Nagoya University   Institute of Transformative Bio-Molecules   Designated associate professor

    2022.4

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    Country:Japan

  2. Nagoya University

    2022.4

  3. Nagoya University   Institute of Transformative Bio-Molecules   Designated lecturer

    2019.4 - 2022.3

  4. JST   PRESTO   PRESTO Researcher

    2018.10 - 2022.3

  5. Nagoya University   Institute of Transformative Bio-Molecules   Visiting Scholar

    2018.10 - 2019.3

  6. Nagoya University   Graduate School of Science   Designated lecturer

    2017.4 - 2018.9

  7. Nagoya University   Graduate School of Science   Designated assistant professor

    2011.1 - 2017.3

  8. JST   ERATO Higashiyama Live-holonics project   Optical Technology Group Group Leader

    2011.1 - 2017.3

  9. 日本学術振興会特別研究員PD

    2009.4 - 2010.12

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    Country:Japan

  10. 日本学術振興会特別研究員DC1

    2006.4 - 2009.3

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    Country:Japan

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Education 3

  1. Osaka University   Graduate School, Division of Engineering

    2006.4 - 2009.3

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    Country: Japan

  2. Osaka University   Graduate School, Division of Engineering

    2004.4 - 2006.3

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    Country: Japan

  3. Osaka University   Faculty of Engineering

    2000.4 - 2004.3

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    Country: Japan

Professional Memberships 3

  1. 日本植物生理学会

  2. 日本植物形態学会

  3. 日本植物学会

Committee Memberships 1

  1. 日本植物形態学会   広報委員長  

    2020.1   

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    Committee type:Academic society

Awards 6

  1. 2016年度JPR論文賞 Best Paper賞

    2016.9   日本植物学会   The carboxyl-terminal tail of the stalk of Arabidopsis NACK1/HINKEL kinesin is required for its localization to the cell plate formation site

    Sasabe M, Ishibashi N, Haruta T, Minami A, Kurihara D, Higashiyama T, Nishihama R, Ito M, Machida Y

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    Award type:Award from Japanese society, conference, symposium, etc.  Country:Japan

  2. 日本植物形態学会 平瀬賞

    2016.9   日本植物形態学会   ClearSee: a rapid optical clearing reagent for whole-plant fluorescence imaging

    Kurihara D., Mizuta Y., Sato Y., Higashiyama T.

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    Award type:Award from Japanese society, conference, symposium, etc.  Country:Japan

  3. Nikon Small World in Motion Competition Honorable Mentions

    2015.12   Nikon Instruments Inc.   Thale cress plant (Arabidopsis thaliana) embryogenesis (30x)

    Daisuke Kurihara

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    Country:United States

  4. 日本植物形態学会 平瀬賞

    2015.9   日本植物形態学会   Rapid Elimination of the Persistent Synergid through a Cell Fusion Mechanism

    Maruyama D., Volz R., Takeuchi H., Mori T., Igawa T., Kurihara D., Kawashima T., Ueda M., Ito M., Umeda M., Nishikawa S., Gross-Hardt R., Higashiyama T.

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    Award type:Award from Japanese society, conference, symposium, etc.  Country:Japan

  5. ITbM Research Award

    2013.10   名古屋大学ITbM   Discovery of New Molecules that Control the Cell Cycle; Understanding the Mechanism of Animal and Plant

    Kurihara D., Kuwata K., Nambo M., Ohkawa T., Ueda M.

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    Country:Japan

  6. 日本植物形態学会奨励賞

    2010.9   日本植物形態学会  

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    Country:Japan

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Papers 58

  1. Dynamics of the cell fate specifications during female gametophyte development in Arabidopsis Reviewed

    Daichi Susaki, Takamasa Suzuki, Daisuke Maruyama, Minako Ueda, Tetsuya Higashiyama, Daisuke Kurihara

    PLOS Biology   Vol. 19 ( 3 ) page: e3001123 - e3001123   2021.3

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    Authorship:Last author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:Public Library of Science (PLoS)  

    The female gametophytes of angiosperms contain cells with distinct functions, such as those that enable reproduction via pollen tube attraction and fertilization. Although the female gametophyte undergoes unique developmental processes, such as several rounds of nuclear division without cell plate formation and final cellularization, it remains unknown when and how the cell fate is determined during development. Here, we visualized the living dynamics of female gametophyte development and performed transcriptome analysis of individual cell types to assess the cell fate specifications in <italic>Arabidopsis thaliana</italic>. We recorded time lapses of the nuclear dynamics and cell plate formation from the 1-nucleate stage to the 7-cell stage after cellularization using an in vitro ovule culture system. The movies showed that the nuclear division occurred along the micropylar–chalazal (distal–proximal) axis. During cellularization, the polar nuclei migrated while associating with the forming edge of the cell plate, and then, migrated toward each other to fuse linearly. We also tracked the gene expression dynamics and identified that the expression of <italic>MYB98pro</italic>::<italic>GFP–MYB98</italic>, a synergid-specific marker, was initiated just after cellularization in the synergid, egg, and central cells and was then restricted to the synergid cells. This indicated that cell fates are determined immediately after cellularization. Transcriptome analysis of the female gametophyte cells of the wild-type and <italic>myb98</italic> mutant revealed that the <italic>myb98</italic> synergid cells had egg cell–like gene expression profiles. Although in <italic>myb98</italic>, egg cell–specific gene expression was properly initiated in the egg cells only after cellularization, but subsequently expressed ectopically in one of the 2 synergid cells. These results, together with the various initiation timings of the egg cell–specific genes, suggest complex regulation of the individual gametophyte cells, such as cellularization-triggered fate initiation, MYB98-dependent fate maintenance, cell morphogenesis, and organelle positioning. Our system of live-cell imaging and cell type–specific gene expression analysis provides insights into the dynamics and mechanisms of cell fate specifications in the development of female gametophytes in plants.

    DOI: 10.1371/journal.pbio.3001123

  2. ClearSeeAlpha: Advanced Optical Clearing for Whole-Plant Imaging Reviewed

    Daisuke Kurihara, Yoko Mizuta, Shiori Nagahara, Tetsuya Higashiyama

    Plant and Cell Physiology     2021.2

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    Authorship:Lead author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:Oxford University Press (OUP)  

    DOI: 10.1093/pcp/pcab033

  3. ClearSee: a rapid optical clearing reagent for whole-plant fluorescence imaging Reviewed

    Kurihara D, Mizuta Y, Sato Y, Higashiyama T

    Development   Vol. 142 ( 23 ) page: 4168-4179   2015.12

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1242/dev.127613

  4. Live-cell imaging and optical manipulation of Arabidopsis early embryogenesis Reviewed

    Gooh K., Ueda M., Aruga K., Park J., Arata H., Higashiyama T., Kurihara D.

    Dev. Cell   Vol. 34   page: 242-251   2015.7

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.devcel.2015.06.008

  5. Chemical screen of Arabidopsis zygote and proteomics in tobacco BY-2 cells identify general plant cell division inhibitors

    Yusuke Kimata, Moé Yamada, Takashi Murata, Keiko Kuwata, Ayato Sato, Takamasa Suzuki, Daisuke Kurihara, Mitsuyasu Hasebe, Tetsuya Higashiyama, Minako Ueda

    bioRxiv     2022.4

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Cold Spring Harbor Laboratory  

    DOI: 10.1101/2022.04.28.489799

  6. Cellular dynamics of endosperm development in Arabidopsis thaliana

    Mohammad Foteh Ali, Ji-Min Shin, Umma Fatema, Daisuke Kurihara, Frédéric Berger, Ling Yuan, Tomokazu Kawashima

    bioRxiv     2022.4

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  7. Optical clearing of plant tissues for fluorescence imaging Invited Reviewed

    Kurihara D, Mizuta Y, Nagahara S, Sato Y, Higashiyama T

    Journal of Visualized Experiments   Vol. 179   2022.1

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    Authorship:Lead author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: doi.org/10.3791/63428

  8. Optical clearing of plant tissues for fluorescence imaging Invited Reviewed

    Kurihara D, Mizuta Y, Nagahara S, Sato Y, Higashiyama T

    Journal of Visualized Experiments   Vol. 179 ( 179 )   2022.1

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    Authorship:Lead author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:MyJove Corporation  

    DOI: 10.3791/63428

  9. A Novel Live Imaging-Based Assay for Visualising Species-Specific Interactions in Gametic Adhesion Molecules

    Kohdai P. Nakajima, Clari Valansi, Daisuke Kurihara, Narie Sasaki, Benjamin Podbilewicz, Tetsuya Higashiyama

    Research Square     2021.12

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    Publisher:Research Square Platform LLC  

    Abstract

    Successful gametic fusion requires species-specific membrane adhesion. However, the interaction of adhesion molecules in gametes is difficult to study in real time through low-throughput microscopic observation. Therefore, we developed a novel live imaging-based adhesion molecule (LIAM) assay to study gametic adhesion molecule interactions in cultured cells. First, we modified a fusion assay previously established for fusogens introduced into cultured cells, and confirmed that our live imaging technique could visualise cell-to-cell fusion in the modified fusion assay. Next, instead of fusogen, we introduced adhesion molecules including a mammalian gametic adhesion molecule pair, IZUMO and JUNO, and detected their temporal accumulation at the contact interfaces of adjacent cells. Accumulated IZUMO or JUNO was translocated to the opposite cells; the mutation in amino acids required for their interaction impaired accumulation and translocation. By using the novel LIAM assay, we investigated the species specificity of IZUMO and JUNO of mouse, human, hamster, and pig in all combinations. IZUMO and JUNO accumulation and translocation were observed in conspecific, and some interspecific, combinations, suggesting potentially interchangeable combinations of IZUMO and JUNO from different species.

    DOI: 10.21203/rs.3.rs-1174354/v1

    Other Link: https://www.researchsquare.com/article/rs-1174354/v1.html

  10. Quantitative Analysis of Plasmodesmata Permeability using Cultured Tobacco BY-2 Cells Entrapped in Microfluidic Chips

    Kazunori Shimizu, Masahiro Kikkawa, Ryo Tabata, Daisuke Kurihara, Ken-ichi Kurotani, Hiroyuki Honda, Michitaka Notaguchi

    bioRxiv     2021.2

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    Publisher:Cold Spring Harbor Laboratory  

    Abstract

    Plasmodesmata are unique channel structures in plants that link the fluid cytoplasm between adjacent cells. Plants have evolved these microchannels to allow trafficking of nutritious substances as well as signaling molecules for intercellular communication. However, tracking the behavior of plasmodesmata in real time is difficult because they are located inside tissues. Hence, we developed a microfluidic device that traps cultured cells and fixes their positions to allow testing of plasmodesmata permeability. The device has 112 tandemly aligned trap zones in the flow channel. Cells of the tobacco line BY-2 were cultured for 7 days and filtered using a sieve and a cell strainer before use to isolate short cell clusters consisting of only a few cells. The isolated cells were introduced into the flow channel, resulting in entrapment of cell clusters at 25 out of 112 trap zones (22.3%). Plasmodesmata permeability was tested from 1 to 4 days after trapping the cells. During this period, the cell numbers increased through cell division. Fluorescence recovery after photobleaching experiments using a transgenic marker line expressing nuclear-localized H2B-GFP demonstrated that cell-to-cell movement of H2B-GFP protein occurred within 200 min of photobleaching. The transport of H2B-GFP protein was not observed when sodium chloride, a compound known to cause plasmodesmata closure, was present in the microfluid channel. Thus, this microfluidic device and one-dimensional plant cell samples allowed us to observe plasmodesmata behavior in real time under controllable conditions.

    DOI: 10.1101/2021.02.19.431975

  11. Mitochondrial dynamics and segregation during the asymmetric division of Arabidopsis zygotes

    Yusuke Kimata, Takumi Higaki, Daisuke Kurihara, Naoe Ando, Hikari Matsumoto, Tetsuya Higashiyama, Minako Ueda

    Quantitative Plant Biology   Vol. 1   2020.11

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Cambridge University Press (CUP)  

    <title>Abstract</title>
    The zygote is the first cell of a multicellular organism. In most angiosperms, the zygote divides asymmetrically to produce an embryo-precursor apical cell and a supporting basal cell. Zygotic division should properly segregate symbiotic organelles, because they cannot be synthesized <italic>de novo</italic>. In this study, we revealed the real-time dynamics of the principle source of ATP biogenesis, mitochondria, in <italic>Arabidopsis thaliana</italic> zygotes using live-cell observations and image quantifications. In the zygote, the mitochondria formed the extended structure associated with the longitudinal array of actin filaments (F-actins) and were polarly distributed along the apical–basal axis. The mitochondria were then temporally fragmented during zygotic division, and the resulting apical cells inherited mitochondria at higher concentration compared to the basal cells. Further observation of postembryonic organs showed that these mitochondrial behaviours are characteristic of the zygote. Overall, our results showed that the zygote has spatiotemporal regulation that unequally distributes the mitochondria.

    DOI: 10.1017/qpb.2020.4

  12. A Peptide Pair Coordinates Regular Ovule Initiation Patterns with Seed Number and Fruit Size Reviewed International journal

    Nozomi Kawamoto, Dunia Pino, Del Carpio, Alexander Hofmann, Yoko Mizuta, Daisuke Kurihara, Tetsuya Higashiyama, Naoyuki Uchida, Keiko U. Torii, Lucia Colombo, Georg Groth, Rüdiger Simon

    Current Biology   Vol. 30 ( 22 ) page: 4352 - 4361.e4   2020.11

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Elsevier BV  

    Ovule development in Arabidopsis thaliana involves pattern formation, which ensures that ovules are regularly arranged in the pistils to reduce competition for nutrients and space. Mechanisms underlying pattern formation in plants, such as phyllotaxis, flower morphogenesis, or lateral root initiation, have been extensively studied, and genes controlling the initiation of ovules have been identified. However, the fundamental patterning mechanism that determines the spacing of ovule anlagen within the placenta remained unexplored. Using natural variation analysis combined with quantitative trait locus analysis, we found that the spacing of ovules in the developing gynoecium and fruits is controlled by two secreted peptides, EPFL2 and EPFL9 (also known as Stomagen), and their receptors from the ERECTA (ER) family that act from the carpel wall and the placental tissue. We found that a signaling pathway controlled by EPFL9 acting from the carpel wall through the LRR-receptor kinases ER, ERL1, and ERL2 promotes fruit growth. Regular spacing of ovules depends on EPFL2 expression in the carpel wall and in the inter-ovule spaces, where it acts through ERL1 and ERL2. Loss of EPFL2 signaling results in shorter gynoecia and fruits and irregular spacing of ovules or even ovule twinning. We propose that the EPFL2 signaling module evolved to control the initiation and regular, equidistant spacing of ovule primordia, which may serve to minimize competition between seeds or facilitate equal resource allocation. Together, EPFL2 and EPFL9 help to coordinate ovule patterning and thereby seed number with gynoecium and fruit growth through a set of shared receptors.

    DOI: 10.1016/j.cub.2020.08.050

    PubMed

  13. Arabidopsis GEX1 Is a Nuclear Membrane Protein of Gametes Required for Nuclear Fusion During Reproduction Reviewed

    Shuh-ichi Nishikawa, Yuki Yamaguchi, Chiharu Suzuki, Ayaka Yabe, Yuzuru Sato, Daisuke Kurihara, Yoshikatsu Sato, Daichi Susaki, Tetsuya Higashiyama, Daisuke Maruyama

    Frontiers in Plant Science   Vol. 11   2020.10

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Frontiers Media SA  

    During the life cycle of flowering plants, nuclear fusion, or karyogamy, occurs three times: once during female gametogenesis, when the two polar nuclei fuse in the central cell, and twice during double fertilization. In Arabidopsis thaliana, nuclear fusion events during sexual reproduction proceed without the breakdown of the nuclear envelope, indicating that nuclear membrane fusion is essential for the completion of this process. Arabidopsis gamete expressed 1 (GEX1) is a membrane protein that is conserved among plant species. GEX1 shares homology with the yeast karyogamy protein Kar5, which is primarily expressed in the nuclear membrane. The GEX1 family represents a putative karyogamy factor. Herein, we show that GEX1 is required for the nuclear fusion events in Arabidopsis reproduction. GEX1-deficient mature female gametophytes were found to contain two unfused polar nuclei in close proximity within the central cell. Electron microscopy showed that the outer membrane of the polar nuclei was connected via the endoplasmic reticulum, whereas the inner membrane remained unfused. These results indicate that GEX1 is involved in polar nuclear membrane fusion following the fusion of the outer nuclear membrane. Furthermore, sperm nuclear fusion events were defective in the fertilized egg and central cell following plasmogamy in the fertilization of gex1-1 female gametophytes by gex1-1 pollen. An analysis of GEX1 localization in the female gametophyte using a transgenic line expressing GFP-tagged GEX1 driven by the GEX1 promoter showed that GEX1 is a nuclear membrane protein in the egg and central cell. Time-lapse live-cell imaging showed that in developing female gametophytes, the nuclear GFP-GEX1 signal was first detectable in the central cell shortly before the polar nuclei came in close contact, and then in the egg cell. Thus, we suggest that the GEX1-family proteins are nuclear membrane proteins involved in karyogamy in the reproduction of eukaryotes including flowering plants.

    DOI: 10.3389/fpls.2020.548032

    Web of Science

  14. The formation of perinucleolar bodies is important for normal leaf development and requires the zinc-finger DNA-binding motif in Arabidopsis ASYMMETRIC LEAVES2. Reviewed International journal

    Lilan Luo, Sayuri Ando, Yuki Sakamoto, Takanori Suzuki, Hiro Takahashi, Nanako Ishibashi, Shoko Kojima, Daisuke Kurihara, Tetsuya Higashiyama, Kotaro T, Yamamoto, Sachihiro Matsunaga, Chiyoko Machida, Michiko Sasabe, Yasunori Machida

    Plant Journal   Vol. 101 ( 5 ) page: 1118 - 1134   2020.3

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    In Arabidopsis, the ASYMMETRIC LEAVES2 (AS2) protein plays a key role in the formation of flat symmetric leaves via direct repression of the abaxial gene ETT/ARF3. AS2 encodes a plant-specific nuclear protein that contains the AS2/LOB domain, which includes a zinc-finger (ZF) motif that is conserved in the AS2/LOB family. We have shown that AS2 binds to the coding DNA of ETT/ARF3, which requires the ZF motif. AS2 is co-localized with AS1 in perinucleolar bodies (AS2 bodies). To identify the amino acid signals in AS2 required for formation of AS2 bodies and function(s) in leaf formation, we constructed recombinant DNAs that encoded mutant AS2 proteins fused to yellow fluorescent protein. We examined the subcellular localization of these proteins in cells of cotyledons and leaf primordia of transgenic plants and cultured cells. The amino acid signals essential for formation of AS2 bodies were located within and adjacent to the ZF motif. Mutant AS2 that failed to form AS2 bodies also failed to rescue the as2-1 mutation. Our results suggest the importance of the formation of AS2 bodies and the nature of interactions of AS2 with its target DNA and nucleolar factors including NUCLEOLIN1. The partial overlap of AS2 bodies with perinucleolar chromocenters with condensed ribosomal RNA genes implies a correlation between AS2 bodies and the chromatin state. Patterns of AS2 bodies in cells during interphase and mitosis in leaf primordia were distinct from those in cultured cells, suggesting that the formation and distribution of AS2 bodies are developmentally modulated in plants.

    DOI: 10.1111/tpj.14579

    PubMed

  15. Live-Cell Imaging of Zygotic Intracellular Structures and Early Embryo Pattern Formation in Arabidopsis thaliana. Reviewed International journal

    Minako Ueda, Yusuke Kimata, Daisuke Kurihara

    Methods in Molecular Biology   Vol. 2122   page: 37 - 47   2020

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    Authorship:Last author, Corresponding author   Language:English   Publishing type:Part of collection (book)  

    Plant embryogenesis begins with fertilization and ends with the generation of the basic body plan of the future plant. Despite its importance, the dynamics of flowering plant ontogeny have long been a mystery, because the embryo develops deep in the maternal tissue. Recently, an embryonic live-cell imaging system was established in Arabidopsis thaliana by developing an in vitro ovule cultivation method and utilizing two-photon excitation microscopy (2PEM), which is suitable for deep imaging. This system enabled us to visualize intracellular dynamics during zygote polarization and monitor the cell division pattern during embryogenesis from the zygote until organ formation. In this chapter, we describe a method that allows for high-resolution imaging of cytoskeletal rearrangements in the zygote and long-term tracing of embryo patterning.

    DOI: 10.1007/978-1-0716-0342-0_4

    PubMed

  16. Polar vacuolar distribution is essential for accurate asymmetric division of Arabidopsis zygotes. Reviewed

    Kimata Y, Kato T, Higaki T, Kurihara D, Yamada T, Segami S, Morita MT, Maeshima M, Hasezawa S, Higashiyama T, Tasaka M, Ueda M

    Proc Natl Acad Sci U S A.   Vol. 116 ( 6 ) page: 2338 - 2343   2019.1

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  17. Spatiotemporal deep imaging of syncytium induced by the soybean cyst nematode Heterodera glycines Reviewed

    Mina Ohtsu, Yoshikatsu Sato, Daisuke Kurihara, Takuya Suzaki, Masayoshi Kawaguchi, Daisuke Maruyama, Tetsuya Higashiyama

    PROTOPLASMA   Vol. 254 ( 6 ) page: 2107 - 2115   2017.11

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:SPRINGER WIEN  

    Parasite infections cause dramatic anatomical and ultrastructural changes in host plants. Cyst nematodes are parasites that invade host roots and induce a specific feeding structure called a syncytium. A syncytium is a large multinucleate cell formed by cell wall dissolution-mediated cell fusion. The soybean cyst nematode (SCN), Heterodera glycines, is a major soybean pathogen. To investigate SCN infection and the syncytium structure, we established an in planta deep imaging system using a clearing solution ClearSee and two-photon excitation microscopy (2PEM). Using this system, we found that several cells were incorporated into the syncytium; the nuclei increased in size and the cell wall openings began to be visible at 2 days after inoculation (DAI). Moreover, at 14 DAI, in the syncytium developed in the cortex, there were thickened concave cell wall pillars that resembled "Parthenon pillars." In contrast, there were many thick board-like cell walls and rarely Parthenon pillars in the syncytium developed in the stele. We revealed that the syncytia were classified into two types based on the pattern of the cell wall structures, which appeared to be determined by the position of the syncytium inside roots. Our results provide new insights into the developmental process of syncytium induced by cyst nematode and a better understanding of the three-dimensional structure of the syncytium in host roots.

    DOI: 10.1007/s00709-017-1105-0

    Web of Science

  18. In Vitro Ovule Cultivation for Live-cell Imaging of Zygote Polarization and Embryo Patterning in Arabidopsis thaliana Invited Reviewed

    Kurihara D, Kimata Y, Higashiyama T, Ueda M

    Journal of Visualized Experiments   Vol. 11 ( 127 )   2017.9

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:JOURNAL OF VISUALIZED EXPERIMENTS  

    In most flowering plants, the zygote and embryo are hidden deep in the mother tissue, and thus it has long been a mystery of how they develop dynamically; for example, how the zygote polarizes to establish the body axis and how the embryo specifies various cell fates during organ formation. This manuscript describes an in vitro ovule culture method to perform live-cell imaging of developing zygotes and embryos of Arabidopsis thaliana. The optimized cultivation medium allows zygotes or early embryos to grow into fertile plants. By combining it with a poly(dimethylsiloxane) (PDMS) micropillar array device, the ovule is held in the liquid medium in the same position. This fixation is crucial to observe the same ovule under a microscope for several days from the zygotic division to the late embryo stage. The resulting live-cell imaging can be used to monitor the real-time dynamics of zygote polarization, such as nuclear migration and cytoskeleton rearrangement, and also the cell division timing and cell fate specification during embryo patterning. Furthermore, this ovule cultivation system can be combined with inhibitor treatments to analyze the effects of various factors on embryo development, and with optical manipulations such as laser disruption to examine the role of cell-cell communication.

    DOI: 10.3791/55975.

    Web of Science

  19. Plant tissue clearing for fluorescence imaging Invited Reviewed

    Plant Morphology   Vol. 29 ( 1 ) page: 81‐86(J‐STAGE) - 86   2017.7

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    Authorship:Lead author, Corresponding author   Language:Japanese  

    DOI: 10.5685/plmorphol.29.81

    J-GLOBAL

  20. イメージング技術を駆使して植物寄生性線虫の感染を捉える Invited Reviewed

    大津美奈, 栗原大輔, 東山哲也

    BSJ-Review   Vol. 8   page: 22 - 28   2017.7

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    Language:Japanese  

  21. Fluorescent Labeling of the Cyst Nematode Heterodera glycines in Deep-Tissue Live Imaging Reviewed

    Ohtsu M, Kurihara D, Sato Y, Suzaki T, Kawaguchi M, Maruyama D, Higashiyama T

    Cytologia   Vol. 82 ( 3 ) page: 251-259   2017.6

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  22. Fluorescent Labeling of the Cyst Nematode Heterodera glycines in Deep-Tissue Live Imaging Reviewed

    Mina Ohtsu, Daisuke Kurihara, Yoshikatsu Sato, Takuya Suzaki, Masayoshi Kawaguchi, Daisuke Maruyama, Tetsuya Higashiyama

    CYTOLOGIA   Vol. 82 ( 3 ) page: 251 - 259   2017.6

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:UNIV TOKYO CYTOLOGIA  

    Nematode infection of plant roots is a paradigm of host parasite interactions. Although nematodes can be labeled with fluorescent dyes, migration of the worms into the deep regions of host roots makes them difficult to track. Here we report the use of two fluorescent dyes, FM4-64 and SYBR green I, to intensely label the soybean cyst nematode (SCN) Heterodera glycines for one week in host plants. Continuous monitoring of the labeled SCN juveniles was achieved with two-photon microscopy. Additionally, we developed a transient transformation system consisting of the non-model leguminous plant (fabaceous) roots, Astragalus sinicus and Agrobacterium rhizogenes to observe the cellular structures of the plant during SCN infection. By the combined use of fluorescent dyes and two-photon microscopy, clear images of infecting SCNs were obtained even in deep regions of A. sinicus roots. The fluorescent labeling described herein can also be used in detailed monitoring of the infection processes of other non-model nematodes, as well as the associated morphological changes in the host plant roots.

    DOI: 10.1508/cytologia.82.251

    Web of Science

  23. Spatiotemporal deep imaging of syncytium induced by the soybean cyst nematode Heterodera glycines Reviewed

    Ohtsu M, Sato Y, Kurihara D, Suzaki T, Kawaguchi M, Maruyama D, Higashiyama T

    Protoplasma     page: .   2017.3

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  24. Cytoskeleton dynamics control the first asymmetric cell division in Arabidopsis zygote Reviewed

    Kimata Y, Higaki T, Kawashima T, Kurihara D, Sato Y, Yamada T, Hasezawa S, Berger F, Higashiyama T, Ueda M

    Proceedings of the National Academy of Sciences   Vol. 113 ( 49 ) page: 14157-14162   2016.12

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  25. Plant Aurora kinases interact with and phosphorylate transcription factors Reviewed

    Takagi M, Sakamoto T, Suzuki R, Nemoto K, Obayashi T, Hirakawa T, Matsunaga TM, Kurihara D, Nariai Y, Urano T, Sawasaki T, Matsunaga S

    J Plant Res   Vol. 129 ( 6 ) page: 1165-1178   2016.11

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    Publishing type:Research paper (scientific journal)  

  26. Combination of Synthetic Chemistry and Live-Cell Imaging Identified a Rapid Cell Division Inhibitor in Tobacco and Arabidopsis thaliana. Reviewed

    Nambo M, Kurihara D, Yamada T, Nishiwaki-Ohkawa T, Kadofusa N, Kimata Y, Kuwata K, Umeda M, Ueda M

    Plant Cell Physiol.   Vol. 57 ( 11 ) page: 2255-2268   2016.11

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  27. Cytokinesis defect in BY-2 cells caused by ATP-competitive kinase inhibitors. Invited Reviewed

    Kozgunova E, Higashiyama T, Kurihara D

    Plant Signal Behav.   Vol. 11 ( 10 ) page: e1238547   2016.10

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  28. Haspin has Multiple Functions in the Plant Cell Division Regulatory Network Reviewed

    Kozgunova E, Suzuki T, Ito M, Higashiyama T, Kurihara D

    Plant Cell Physiol.     page: in press   2016.2

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    DOI: in press

  29. Visualization of Plant Sexual Reproduction in the Whole-mount Pistil by ClearSee Reviewed

    Yoko Mizuta, Daisuke Kurihara, Tetsuya Higashiyama

    Cytologia   Vol. 81 ( 1 ) page: 1-2   2016.1

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  30. Rapid elimination of the persistent synergid through a cell fusion mechanism Reviewed

    Maruyama D, Völz R, Takeuchi H, Mori T, Igawa T, Kurihara D, Kawashima T, Ueda M, Ito M, Umeda M, Nishikawa S, Groß-Hardt R, Higashiyama T

    Cell   Vol. 161   page: 907-918   2015.5

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    DOI: 10.1016/j.cell.2015.03.018

  31. Live imaging and laser disruption reveal the dynamics and cell-cell communication during Torenia fournieri female gametophyte development Reviewed

    Susaki D, Takeuchi H, Tsutsui H, Kurihara D, Higashiyama T

    Plant Cell Physiol   Vol. 56   page: 1031-1041   2015.5

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    DOI: 10.1093/pcp/pcv031

  32. Two-photon imaging with longer wavelength excitation in intact Arabidopsis tissues Reviewed

    Mizuta Y, Kurihara D, Higashiyama T.

    Protoplasma     page: 1-10   2015.1

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  33. The carboxyl-terminal tail of the stalk of Arabidopsis NACK1/HINKEL kinesin is required for its localization to the cell plate formation site Reviewed

    Sasabe M, Ishibashi N, Haruta T, Minami A, Kurihara D, Higashiyama T, Nishihama R, Ito M, Machida Y

    Journal of Plant Research     page: 1-10   2014.12

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  34. Increase in invaginated vacuolar membrane structure caused by plant cell expansion by genotoxic stress induced by DNA double-strand breaks Reviewed

    Hasegawa J, Higaki T, Hamamura Y, Kurihara D, Kutsuna N, Higashiyama T, Hasezawa S, Matsunaga S

    Cytologia   Vol. 79 ( 4 ) page: 467-474   2014.12

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  35. Live imaging of calcium spikes during double fertilization in Arabidopsis. Reviewed

    Hamamura Y, Nishimaki M, Takeuchi H, Geitmann A, Kurihara D, Higashiyama T.

    Nat Commun.   Vol. 5   page: 4722   2014.8

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    Ca(2+) waves and oscillation are key signalling elements during the fertilization process of animals, and are involved, for example, in egg activation. In the unique double fertilization process in flowering plants, both the egg cell and the neighbouring central cell fuse with a sperm cell each. Here we succeeded in imaging cytosolic Ca(2+) in these two cells, and in the two synergid cells that accompany the gametes during semi-in vivo double fertilization. Following pollen tube discharge and plasmogamy, the egg and central cells displayed transient Ca(2+) spikes, but not oscillations. Only the events in the egg cell correlated with the plasmogamy. In contrast, the synergid cells displayed Ca(2+) oscillations on pollen tube arrival. The two synergid cells showed distinct Ca(2+) dynamics depending on their respective roles in tube reception. These Ca(2+) dynamics in the female gametophyte seem to represent highly specific signatures that coordinate successful double fertilization in the flowering plants.

    DOI: 10.1038/ncomms5722.

  36. Fabrication of microcage arrays to fix plant ovules for long-term live imaging and observation Reviewed

    Park J., Kurihara D., Higashiyama T., Arata H.

    Sensors & Actuators B: Chemical   Vol. 191   page: 178–185   2014.2

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    DOI: 10.1016/j.snb.2013.09.060

  37. 2光子顕微鏡による植物深部のin vivoイメージング Invited

    水多陽子, 栗原大輔, 東山哲也

      Vol. 26   page: 25-30   2014

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  38. Kinetochore and microtubule dynamics during cell division of tobacco BY-2 cells visualized by live-cell imaging

    Kurihara D, Matsunaga S

    Atlas of Plant Cell Structure     page: 16-17   2014

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  39. Live-cell analysis of plant reproduction: live-cell imaging, optical manipulation, and advanced microscopy technologies Invited Reviewed

    Daisuke Kurihara, Yuki Hamamura, Tetsuya Higashiyama

    Dev Growth Differ.   Vol. 55 ( 4 ) page: 462-473   2013.5

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    Sexual reproduction ensures propagation of species and enhances genetic diversity within populations. In flowering plants, sexual reproduction requires complicated and multi-step cell-to-cell communications among male and female cells. However, the confined nature of plant reproduction processes, which occur in the female reproductive organs and several cell layers of the pistil, limits our ability to observe these events in vivo. In this review, we discuss recent live-cell imaging in in vitro systems and the optical manipulation techniques that are used to capture the dynamic mechanisms representing molecular and cellular communications in sexual plant reproduction.

    DOI: 10.1111/dgd.12040

  40. Independent control by each female gamete prevents the attraction of multiple pollen tubes. Reviewed

    Maruyama D, Hamamura Y, Takeuchi H, Susaki D, Nishimaki M, Kurihara D, Kasahara RD, Higashiyama T.

    Dev Cell   Vol. 25 ( 3 ) page: 317-323   2013.5

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    In flowering plants, double fertilization is normally accomplished by the first pollen tube, with the fertilized ovule subsequently inhibiting the attraction of a second pollen tube. However, the mechanism of second-pollen-tube avoidance remains unknown. We discovered that failure to fertilize either the egg cell or the central cell compromised second-pollen-tube avoidance in Arabidopsis thaliana. A similar disturbance was caused by disrupting the fertilization-independent seed (FIS) class polycomb-repressive complex 2 (FIS-PRC2), a central cell- and endosperm-specific chromatin-modifying complex for gene silencing. Therefore, the two female gametes have evolved their own signaling pathways. Intriguingly, second-pollen-tube attraction induced by half-successful fertilization allowed the ovules to complete double fertilization, producing a genetically distinct embryo and endosperm. We thus propose that each female gamete independently determines second-pollen-tube avoidance to maximize reproductive fitness in flowering plants.

    DOI: 10.1016/j.devcel.2013.03.013.

  41. Arabidopsis ASYMMETRIC LEAVES2 protein required for leaf morphogenesis consistently forms speckles during mitosis of tobacco BY-2 cells via signals in its specific sequence Reviewed

    Luo L, Ando S, Sasabe M, Machida C, Kurihara D, Higashiyama T, Machida Y

    J Plant Res.   Vol. 125 ( 5 ) page: 661-668   2012.9

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  42. Live-Cell Imaging of Double Fertilization and Embryogenesis in Plants Reviewed

    Daisuke Kurihara, Yuki Hamamura, Tetsuya Higashiyama

    Conference Proceedings APMC 10 / ICONN 2012 / ACMM 22   Vol. 1055   page: 1-2   2012.2

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  43. Programmed induction of endoreduplication by DNA double-strand breaks in Arabidopsis Reviewed

    Adachi S, Minamisawa K, Okushima Y, Inagaki S, Yoshiyama K, Kondou Y, Kaminuma E, Kawashima M, Toyoda T, Matsui M, Kurihara D, Matsunaga S, Umeda M

    Proc Natl Acad Sci U S A.   Vol. 108 ( 24 ) page: 10004-10009   2011.6

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  44. Live-cell imaging reveals the dynamics of two sperm cells during double fertilization in Arabidopsis thaliana Reviewed

    Hamamura Y, Saito C, Awai C, Kurihara D, Miyawaki A, Nakagawa T, Kanaoka MM, Sasaki N, Nakano A, Berger F, Higashiyama T

    Curr Biol.   Vol. 21 ( 6 ) page: 497-502   2011.5

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  45. 細胞分裂期において分裂期キナーゼが制御する染色体動態 Invited

    栗原大輔

      Vol. 23   page: 81-89   2011.5

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  46. Identification and characterization of plant Haspin kinase as a histone H3 threonine kinase Reviewed

    Kurihara D, Matsunaga S, Omura T, Higashiyama T, Fukui K

    BMC Plant Biol.   Vol. 11   page: 73   2011.4

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  47. Chemical visualization of an attractant peptide, LURE. Reviewed

    Goto H, Okuda S, Mizukami A, Mori H, Sasaki N, Kurihara D, Higashiyama T

    Plant Cell Physiol.   Vol. 52 ( 1 ) page: 49-58   2011.1

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  48. Live cell imaging reveals plant aurora kinase has dual roles during mitosis Reviewed

    Kurihara D, Matsunaga S, Uchiyama S, Fukui K

    Plant Cell Physiol.   Vol. 49 ( 8 ) page: 1256-1261   2008.8

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  49. Visualization of mitotic HeLa cells by advanced polarized light microscopy Reviewed

    Morimoto A, Matsunaga S, Kurihara D, Fukui K

    Micron   Vol. 39 ( 3 ) page: 635-638   2008.6

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  50. The Arabidopsis SDG4 contributes to the regulation of pollen tube growth by methylation of histone H3 lysines 4 and 36 in mature pollen Reviewed

    Cartagena JA, Matsunaga S, Seki M, Kurihara D, Yokoyama M, Shinozaki K, Fujimoto S, Azumi Y, Uchiyama S, Fukui K

    Dev Biol.   Vol. 315 ( 2 ) page: 355-368   2008.5

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  51. Plant Aurora kinase has dual roles on chromosome alignment and segregation during mitosis Reviewed

    Kurihara D, Matsunaga S, Uchiyama S, Fukui K

    Cytologia   Vol. 73   page: 1-2   2008

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  52. PHB2 protects sister-chromatid cohesion in mitosis Reviewed

    Takata H, Matsunaga S, Morimoto A, Ma N, Kurihara D, Ono-Maniwa R, Nakagawa M, Azuma T, Uchiyama S, Fukui K

    Curr Biol.   Vol. 17 ( 15 ) page: 1356-1361   2007.8

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  53. Single-organelle tracking by two-photon conversion Reviewed

    Watanabe W, Shimada T, Matsunaga S, Kurihara D, Fukui K, Shin-Ichi Arimura S, Tsutsumi N, Isobe K, Itoh K

    Opt Express   Vol. 15 ( 5 ) page: 2490-2498   2007.5

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  54. Characterization of a splicing variant of plant Aurora kinase Reviewed

    Kurihara D, Kawabe A, Matsunaga S, Nakagawa K, Fujimoto S, Uchiyama S, Fukui K

    Plant Cell Physiol.   Vol. 48 ( 2 ) page: 369-374   2007.2

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  55. Aurora kinase is required for chromosome segregation in tobacco BY-2 cells Reviewed

    Kurihara D, Matsunaga S, Kawabe A, Fujimoto S, Noda M, Uchiyama S, Fukui K

    Plant J.   Vol. 48 ( 4 ) page: 572-580   2006.11

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  56. Chromosome dynamics in Arabidopsis Invited Reviewed

    Kurihara D, Matsunaga S, Fukui K

    Plant Genome: Biodiversity and Evolution     page: 127-147   2006

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  57. Characterization of plant Aurora kinases during mitosis Reviewed

    Kawabe A, Matsunaga S, Nakagawa K, Kurihara D, Yoneda A, Hasezawa S, Uchiyama S, Fukui K

    Plant Mol. Biol.   Vol. 58 ( 1 ) page: 1-13   2005.5

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  58. Dynamics of the plant Aurora kinase AtAUR3 during mitosis and spindle abnormality induced by AtAUR3 overexpression Reviewed

    Matsunaga S, Kurihara D, Kawabe A, Nakagawa K, Yoneda A, Hasezawa S, Uchiyama S, Fukui K

    Cytologia   Vol. 70   page: 1-2   2005

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    Authorship:Lead author   Language:English  

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Books 2

  1. Plant Embryogenesis

    Minako Ueda, Daisuke Kurihara( Role: Joint editor)

    2021.8  ( ISBN:3036514619

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    Total pages:124  

  2. エッセンシャル遺伝学・ゲノム科学

    栗原大輔, 植田美那子, 水多陽子( Role: Joint translator ,  原著第7版, 第9章)

    化学同人  2021.1  ( ISBN:9784759820485

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    Total pages:xxvi, 523p   Language:Japanese

    CiNii Books

MISC 21

  1. Analyses of aberrant embryo development observed in the Arabidopsis mutant defective in the nuclear fusion during reproduction

    西川周一, 高木祐理, 佐藤譲, 栗原大輔, 栗原大輔, 佐藤良勝, 東山哲也, 東山哲也, 東山哲也, 丸山大輔

    日本植物生理学会年会(Web)   Vol. 62nd   2021

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  2. Spatio-temporal visualization of syncytium formation during cyst nematode infection

    大津美奈, 大津美奈, 佐藤良勝, 佐藤良勝, 栗原大輔, 丸山大輔, 東山哲也, 東山哲也

    Nematological Research   Vol. 51 ( 2 )   2021

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  3. Challenges to Deep Imaging of the Whole Plant Invited Reviewed

    顕微鏡   Vol. 55 ( 3 ) page: 146 - 151   2020

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    Authorship:Lead author, Corresponding author  

    J-GLOBAL

  4. 植物における受精卵の極性化動態のライブイメージング

    植田美那子, 植田美那子, 植田美那子, 木全祐資, 松本光梨, 檜垣匠, 小松大鳳, 田中小百合, 栗原大輔, 栗原大輔, 東山哲也, 東山哲也, 東山哲也

    日本分子生物学会年会プログラム・要旨集(Web)   Vol. 43rd   2020

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  5. シロイヌナズナ新規核膜融合因子Gex1の細胞内動態の解析

    鈴木千晴, 矢部あやか, 栗原大輔, 栗原大輔, 佐藤良勝, 東山哲也, 東山哲也, 東山哲也, 西川周一

    日本植物学会大会研究発表記録   Vol. 83rd   2019

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  6. 光細胞操作を駆使し,受精因子の機能を探る

    中島耕大, 永原史織, 佐々木妙子, VALANSI Clari, 栗原大輔, 佐藤良勝, 佐々木成江, PODBILEWICZ Benjamin, 東山哲也, 東山哲也

    Plant Morphology   Vol. 31 ( 1 )   2019

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  7. 光ピンセットを用いた細胞操作による受精因子解析の試み

    中島耕大, 永原史織, 佐々木妙子, VALANSI Clari, 栗原大輔, 佐藤良勝, 佐々木成江, PODBILEWICZ Benjamin, 東山哲也, 東山哲也

    日本植物学会大会研究発表記録   Vol. 82nd   2018

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  8. イメージング技術を駆使して植物寄生性線虫の感染を捉える Invited Reviewed

    大津美奈, 栗原大輔, 東山哲也

    BSJ-Review   Vol. 8   page: 22 - 28   2017.7

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  9. イメージング技術を駆使して植物寄生性線虫の感染を捉える Invited Reviewed

    大津美奈, 栗原大輔, 東山哲也

    BSJ-Review   Vol. 8   page: 22 - 28   2017.7

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  10. シロイヌナズナ受精卵の極性化動態

    木全祐資, 栗原大輔, 栗原大輔, 栗原大輔, 桧垣匠, 河島友和, 佐藤良勝, 山田朋美, BERGER Frederic, 馳澤盛一郎, 東山哲也, 東山哲也, 東山哲也, 植田美那子, 植田美那子

    Plant Morphology   Vol. 29 ( 1 )   2017

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  11. 受精卵の不等分裂を制御する細胞骨格ダイナミクス

    木全祐資, 檜垣匠, 河島友和, 河島友和, 栗原大輔, 栗原大輔, 佐藤良勝, 山田朋美, 山田朋美, 馳澤盛一郎, BERGER Frederic, 東山哲也, 東山哲也, 東山哲也, 植田美那子, 植田美那子

    日本植物学会大会研究発表記録   Vol. 81st   2017

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  12. シロイヌナズナ花粉管誘引のライブイメージング

    時田公美, 水多陽子, 水多陽子, 栗原大輔, 東山哲也, 東山哲也

    日本植物学会大会研究発表記録   Vol. 81st   2017

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  13. 植物を丸ごと透明化し,中まで蛍光観察する新技術を開発 細胞レベルでの個体全体の観察を目指して Invited Reviewed

    栗原大輔

    化学と生物   Vol. 54 ( 11 ) page: 794 - 796   2016.10

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  14. 植物組織透明化試薬ClearSeeの開発 Invited

    栗原大輔

    和光純薬時報     2016.10

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  15. 植物研究における2光子励起顕微鏡の活用 Invited Reviewed

    栗原大輔

    BSJ-Review   Vol. 7   page: 124 - 130   2016.5

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  16. 植物の透明化試薬「クリアシー」で線虫の侵入状況など観察可能に Invited

    栗原大輔

    ニューカントリー     page: 56 - 57   2016.4

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  17. 透明にすることによって見えてくるもの Invited

    栗原大輔

    現代化学   ( 539 ) page: 43 - 47   2016.2

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  18. 2光子励起顕微鏡を用いた生体深部イメージングと光顕微操作 Invited Reviewed

    水多陽子, 栗原大輔

    植物の生長調節   Vol. 49 ( 2 ) page: 96 - 103   2014.12

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    Authorship:Last author, Corresponding author   Language:Japanese   Publishing type:Article, review, commentary, editorial, etc. (scientific journal)  

  19. 植物染色体の最前線 染色体動態―染色体の動き,動物と植物の共通点と違い Invited

    栗原大輔, 松永幸大

    生物の科学 遺伝   Vol. 63 ( 3 ) page: 55 - 60   2009.5

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  20. 植物のヒストン翻訳後修飾研究の幕開け 分化発生,形態形成,ストレス応答を制御する“第2のコード”の意味とは?

    松永幸大, 栗原大輔, 中園幹生

    化学と生物   Vol. 44 ( 12 ) page: 798 - 700   2006.12

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  21. Chromosome dynamics in Arabidopsis Reviewed

    Kurihara D, Matsunaga S, Fukui K

    Plant Genome: Biodiversity and Evolution     page: 127 - 147   2006

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Presentations 103

  1. Cell fate specifications during female gametophyte development in Arabidopsis Invited

    Daisuke Kurihara

    55th Annual Meeting of JSDB  2022.6.1 

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    Event date: 2022.5 - 2022.6

    Language:English   Presentation type:Oral presentation (invited, special)  

  2. 植物雌性配偶体をモデルとした細胞運命制御機構の解明 Invited

    栗原大輔

    有性生殖における染色体・クロマチン・核動態に関する若手研究者の会  2022.4.15  遺伝研研究会

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    Event date: 2022.4

    Language:Japanese   Presentation type:Public lecture, seminar, tutorial, course, or other speech  

  3. 植物雌性配偶体をモデルとした細胞運命制御機構の解明 Invited

    栗原大輔

    有性生殖における染色体・クロマチン・核動態に関する若手研究者の会  2022.4.15  遺伝研研究会

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    Event date: 2022.4

    Language:Japanese   Presentation type:Public lecture, seminar, tutorial, course, or other speech  

  4. 植物組織のライブイメージング・深部イメージングに向けて Invited

    栗原大輔

    第63回日本植物生理学会年会 ランチョンセミナー  2022.3.23  オリンパス株式会社

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    Event date: 2022.3

    Language:Japanese   Presentation type:Public lecture, seminar, tutorial, course, or other speech  

  5. 植物組織のライブイメージング・深部イメージングに向けて Invited

    栗原大輔

    第63回日本植物生理学会年会 ランチョンセミナー  2022.3.23  オリンパス株式会社

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    Event date: 2022.3

    Language:Japanese   Presentation type:Public lecture, seminar, tutorial, course, or other speech  

  6. 透明化蛍光観察で気をつけること Invited

    栗原大輔

    日本植物学会関連集会 植物イメージングに欠かせない知識と技術3  2021.9.18 

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    Event date: 2021.9

    Presentation type:Oral presentation (invited, special)  

  7. トマト果実におけるGCaMP6用いたカルシウムイメージング

    堀千秋, 栗原大輔, 大村道明, 西山学, 金山喜則, 加藤一幾

    園芸学会令和3年度春季大会  2021.3.27 

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    Event date: 2021.3

    Presentation type:Oral presentation (general)  

  8. シロイヌナズナ核融合欠損株で観察される受精後の胚発生異常の解析

    西川周一, 高木祐理, 佐藤譲, 栗原大輔, 佐藤良勝, 東山哲也, 丸山大輔

    第62回日本植物生理学会年会  2021.3.14 

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    Event date: 2021.3

    Presentation type:Oral presentation (general)  

  9. 植物初期発生過程における細胞運命制御機構の解明 Invited

    栗原大輔

    『配偶子インテグリティの構築』『全能性プログラム』合同公開シンポジウム  2020.12.21 

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    Event date: 2020.12

    Presentation type:Oral presentation (general)  

  10. 葉形成に関与するAS2タンパク質の動態変化と機能の関係

    笹部美知子, 雪森桃花, 吉田みのり, 三石萌, 小島晶子, 栗原大輔, 東山哲也, 町田千代子, 町田泰則

    日本植物学会第84回大会  2020.9.20 

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    Event date: 2020.9

    Presentation type:Oral presentation (general)  

  11. 受精卵の内部動態から迫る植物の体軸形成機構 Invited

    植田 美那子, 木全 祐資, 松本 光梨, 檜垣 匠, 栗原 大輔, 東山 哲也

    日本植物学会第84回大会  2020.9.19 

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    Event date: 2020.9

    Presentation type:Oral presentation (general)  

  12. 花粉不発芽を引き起こすDPL遺伝子の機能解析

    水多陽子, 栗原大輔

    日本植物学会第84回大会  2020.9.19 

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    Event date: 2020.9

    Presentation type:Oral presentation (general)  

  13. 動物培養細胞を用いた動植物の受精因子群の接着性の検証

    中島耕大, Clari Valansi, 栗原大輔, 佐々木成江, Benjamin Podbilewicz, 東山哲也

    日本植物学会第84回大会  2020.9.19 

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    Event date: 2020.9

    Presentation type:Oral presentation (general)  

  14. シロイヌナズナ雌性配偶体細胞における運命決定のダイナミクス

    栗原大輔, 須崎大地, 海老根一生, 鈴木孝征, 丸山大輔, 植田美那子, 東山哲也

    日本植物学会第84回大会  2020.9.19 

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    Event date: 2020.9

    Presentation type:Oral presentation (general)  

  15. シロイヌナズナ雌性配偶体細胞のRNA-seq解析

    須崎大地, 栗原大輔, 鈴木孝征, 丸山大輔, 植田美那子, 東山哲也

    日本植物学会第84回大会  2020.9.21 

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    Event date: 2020.9

    Presentation type:Poster presentation  

  16. GCaMP6を用いたカルシウムイメージングのトマトへの応用

    堀千秋, 伊藤輝, 栗原大輔, 石田宏幸, 大村道明, 金山喜則, 加藤一幾

    園芸学会令和2年度春季大会  2020.3.21 

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    Event date: 2020.3

    Presentation type:Oral presentation (general)  

  17. Live imaging and optical manipulation of plant reproduction at a single cell level Invited

    Daisuke Kurihara

    Live imaging and optical manipulation of plant reproduction at a single cell level  2017.3.18 

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    Event date: 2017.3

    Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  18. ClearSee: a rapid optical clearing reagent for whole-plant fluorescence imaging International conference

    Daisuke Kurihara, Yoko Mizuta, Yoshikatsu Sato, Tetsuya Higashiyama

    The 1st ABiS Symposium - Towards the Future of Advanced Bioimaging for Life Sciences -  2017.2.19 

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    Event date: 2017.2

    Presentation type:Poster presentation  

    Country:Japan  

  19. 植物のからだを覗いてみよう Invited International conference

    栗原大輔

    名古屋大学出前授業in豊橋 

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    Event date: 2016.12

    Country:Japan  

  20. 今、顕微鏡技術で観えるもの Invited International conference

    栗原大輔

    第12回関西創農薬研究会 

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    Event date: 2016.11

    Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  21. 植物深部に侵入した微生物を光学顕微鏡で観察する Invited International conference

    栗原大輔,大津美奈,水多陽子,東山哲也

    日本植物学会第80回大会 

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    Event date: 2016.9

    Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  22. ClearSee: a rapid optical clearing reagent for whole-plant fluorescence imaging Invited International conference

    栗原大輔,水多陽子,佐藤良勝,東山哲也

    日本植物形態学会第28回大会 

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    Event date: 2016.9

    Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  23. Cell fate specification in Arabidopsis female gametophyte development International conference

    Daisuke Kurihara, Daichi Susaki, Tetsuya Higashiyama

    the 24th International Congress on Sexual Plant Reproduction 

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    Event date: 2016.3

    Language:English   Presentation type:Poster presentation  

    Country:United States  

  24. Live cell analysis and deep imaging for plant development International conference

    Daisuke KURIHARA

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    Event date: 2016.3

    Language:English   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  25. 顕微鏡技術で切り拓く植物発生研究

    栗原大輔

    第41回植物バイテクシンポジウム 

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    Event date: 2016.1

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Venue:京都産業大学   Country:Japan  

  26. 深部観察で Whole plant imaging を目指す

    栗原大輔,水多陽子,佐藤良勝,東山哲也

    日本植物学会第79回大会 

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    Event date: 2015.9

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Venue:朱鷺メッセ・新潟コンベンションセンター   Country:Japan  

  27. Live-cell imaging and optical manipulation of Arabidopsis early embryogenesis International conference

    Daisuke Kurihara

    International ERATO Higashiyama Live-Holonics Symposium 2015 

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    Event date: 2015.8

    Language:English   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  28. シロイヌナズナ初期胚における細胞運命決定機構の解析

    栗原大輔,牛王啓太,有賀花奈,植田美那子,東山哲也

    日本植物学会第78回大会 

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    Event date: 2014.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:明治大学生田キャンパス   Country:Japan  

  29. シロイヌナズナ雌性配偶体発生における細胞個性獲得変異体の解析

    栗原 大輔,東山 哲也

    日本植物形態学会第26回総会・大会 

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    Event date: 2014.9

    Language:Japanese   Presentation type:Poster presentation  

    Venue:明治大学生田キャンパス   Country:Japan  

  30. Live-cell analysis of Arabidopsis early embryogenesis International conference

    Daisuke Kurihara

    International ERATO Higashiyama Live-Holonics Symposium 2014 

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    Event date: 2014.9

    Language:English   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  31. Live-cell analysis to reveal the cell fate determination during early embryogenesis in Arabidopsis thaliana International conference

    Daisuke Kurihara, Keita Gooh, Minako Ueda, Tetsuya Higashiyama

    23rd International Congress on Sexual Plant Reproduction 

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    Event date: 2014.7

    Language:English   Presentation type:Poster presentation  

    Country:Portugal  

  32. 光顕微操作で植物胚発生に迫る International conference

    栗原大輔

    シンポジウム「細胞を創る操る」 

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    Event date: 2013.11

    Language:English   Presentation type:Oral presentation (invited, special)  

    Venue:奈良先端科学技術大学院大学   Country:Japan  

  33. マイクロデバイスを用いたシロイヌナズナ胚発生過程のライブイメージング

    栗原大輔,牛王啓太,朴鍾淏,新田英之,東山哲也

    日本植物学会第77回大会 

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    Event date: 2013.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:北海道大学 高等教育推進機構   Country:Japan  

  34. シロイヌナズナ胚発生過程のライブイメージング―マイクロデバイスを用いたアプローチ

    栗原大輔,牛王啓太,朴鍾淏,新田英之,東山哲也

    日本植物形態学会第25回総会・大会 

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    Event date: 2013.9

    Language:Japanese   Presentation type:Poster presentation  

    Venue:北海道大学札幌キャンパス   Country:Japan  

  35. 光顕微操作とライブイメージングで捉える植物胚発生

    栗原大輔

    2013年度細胞周期合同セミナー 

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    Event date: 2013.6

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:静岡・KKR伊豆長岡千歳荘   Country:Japan  

  36. Live-cell analysis of embryogenesis in Arabidopsis thaliana International conference

    Daisuke Kurihara, Keita Gooh, Tetsuya Higashiyama

    the 24th International Conference on Arabidopsis Research 

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    Event date: 2013.2

    Language:English   Presentation type:Poster presentation  

    Country:Australia  

  37. 組織深部のライブイメージングと光細胞操作で花の内部を探る

    東山哲也、栗原大輔

    日本女子大学バイオイメージングセンター第4回公開シンポジウム 

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    Event date: 2012.10

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Venue:日本女子大学八十年館   Country:Japan  

  38. 光顕微操作とライブイメージングで迫るパターン形成

    栗原大輔, 牛王啓太, 東山哲也

    日本植物学会第76回大会 

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    Event date: 2012.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:兵庫県立大学書写キャンパス   Country:Japan  

  39. ホロニックコミュニケーションを担うシグナリング分子の可視化に向けて

    栗原大輔, 東山哲也

    日本植物形態学会第24回総会・大会 

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    Event date: 2012.9

    Language:Japanese   Presentation type:Poster presentation  

    Venue:兵庫県立大学書写キャンパス   Country:Japan  

  40. 光顕微操作とライブイメージングで迫る植物胚発生

    栗原大輔

    第53回日本植物生理学会年会 

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    Event date: 2012.3

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Venue:京都産業大学   Country:Japan  

  41. Live-Cell Imaging of Double Fertilization and Embryogenesis in Plants International conference

    Daisuke Kurihara, Yuki Hamamura, Tetsuya Higashiyama

    the 10th Asia-Pacific Microscopy Conference (APMC 10), the 2012 International Conference on Nanoscience and Nanotechnology (ICONN 2012) and the 22nd Australian Conference on Microscopy and Microanalysis (ACMM 22) 

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    Event date: 2012.2

    Language:English   Presentation type:Oral presentation (invited, special)  

    Country:Australia  

  42. シロイヌナズナ胚発生過程のライブイメージング

    栗原大輔, 牛王啓太, 東山哲也

    日本植物学会第75回大会 

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    Event date: 2011.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:東京大学駒場キャンパス   Country:Japan  

  43. 光操作技術によるシロイヌナズナ胚発生過程の解析

    栗原大輔, 牛王啓太, 東山哲也

    日本植物形態学会第23回総会・大会 

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    Event date: 2011.9

    Language:Japanese   Presentation type:Poster presentation  

    Venue:日本女子大学目白キャンパス   Country:Japan  

  44. Live-cell imaging of embryo development in Arabidopsis thaliana

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    Event date: 2011.6

    Language:Japanese   Presentation type:Poster presentation  

    Country:Japan  

  45. 細胞分裂におけるハスピンキナーゼの解析

    栗原大輔, 松永幸大, 大村知広, 東山哲也, 福井希一

    日本植物学会第74回大会 

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    Event date: 2010.9

    Language:Japanese   Presentation type:Poster presentation  

    Venue:中部大学春日井キャンパス   Country:Japan  

  46. シロイヌナズナの細胞分裂に関わるヒストンH3スレオニンキナーゼの解析

    栗原大輔, 松永幸大, 大村知広, 東山哲也, 福井希一

    日本植物形態学会第22回総会・大会 

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    Event date: 2010.9

    Language:Japanese   Presentation type:Poster presentation  

    Venue:中部大学春日井キャンパス   Country:Japan  

  47. 分裂期キナーゼが制御する染色体動態からみた細胞分裂の研究

    栗原大輔

    日本植物形態学会第22回総会・大会 

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    Event date: 2010.9

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Venue:中部大学春日井キャンパス   Country:Japan  

  48. 細胞分裂期におけるヒストンH3スレオニンキナーゼの同定および解析

    栗原大輔, 松永幸大, 大村知広, 内山進, 福井希一

    日本植物学会第73回大会 

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    Event date: 2009.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:山形大学小白川キャンパス   Country:Japan  

  49. 染色体動態を制御するオーロラキナーゼの機能解析

    栗原大輔, 松永幸大, 池田虎三, 内山進, 福井希一

    日本植物形態学会第21回総会・大会 

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    Event date: 2009.9

    Language:Japanese   Presentation type:Poster presentation  

    Venue:山形大学小白川キャンパス   Country:Japan  

  50. Functional analysis of plant Aurora kinase during mitosis International conference

    Daisuke Kurihara, Susumu Uchiyama, Sachihiro Matsunaga, Kiichi Fukui

    The 3rd Asian Chromosome Colloquium 

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    Event date: 2008.12

    Language:English   Presentation type:Oral presentation (general)  

    Country:Japan  

  51. 体細胞分裂におけるAuroraキナーゼの機能解析

    栗原大輔

    特定領域研究「植物メリステム」若手ワークショップ 

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    Event date: 2008.11

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:奈良県社会教育センター かつらぎ   Country:Japan  

  52. Characterization of a splicing variant of plant Aurora kinase

    Daisuke Kurihara, Akira Kawabe, Katsuyuki Nakagawa, Satoru Fujimoto, Susumu Uchiyama, Sachihiro Matsunaga, Kiichi Fukui

    The 1st International Global COE symposium on Global Education and Bio-Environmental Chemistry(GCOEBEC-1) 

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    Event date: 2008.1

    Language:English   Presentation type:Poster presentation  

    Country:Japan  

  53. Characterization of a splicing variant of plant Aurora kinase

    Daisuke Kurihara, Akira Kawabe, Katsuyuki Nakagawa, Satoru Fujimoto, Susumu Uchiyama, Sachihiro Matsunaga, Kiichi Fukui

    The 1st International Global COE symposium on Global Education and Bio-Environmental Chemistry(GCOEBEC-1) 

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    Event date: 2008.1

    Language:English   Presentation type:Oral presentation (general)  

    Country:Japan  

  54. 可視化技術による植物Auroraキナーゼの機能解析

    栗原大輔, 内山進, 松永幸大, 福井希一

    第30回日本分子生物学会年会・第80回日本生化学会大会 合同大会 

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    Event date: 2007.12

    Language:Japanese   Presentation type:Poster presentation  

    Venue:パシフィコ横浜・ヨコハマグランドインターコンチネンタルホテル   Country:Japan  

  55. 植物において染色体分離を制御するAuroraキナーゼ

    栗原大輔, 野田勝紀, 河邊昭, 藤本聡, 内山進, 松永幸大, 福井希一

    日本遺伝学会第79回大会 

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    Event date: 2007.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:岡山大学理学部・津島キャンパス   Country:Japan  

  56. Functional analysis of plant Aurora kinases in chromosome segregation during mitosis International conference

    Daisuke Kurihara

    Genetisches Seminar 

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    Event date: 2007.8

    Language:English   Presentation type:Oral presentation (invited, special)  

    Country:Germany  

  57. Aurora kinase is required for chromosome segregation in tobacco BY-2 cells International conference

    Daisuke Kurihara, Masanori Noda, Akira Kawabe, Satoru Fujimoto, Susumu Uchiyama, Sachihiro Matsunaga, Kiichi Fukui

    16th International Chromosome Conference 

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    Event date: 2007.8

    Language:English   Presentation type:Poster presentation  

    Country:Netherlands  

  58. 植物においてヒストンH3をリン酸化するAuroraキナーゼ

    栗原大輔, 野田勝紀, 河邊昭, 藤本聡, 内山進, 松永幸大, 福井希一

    第1回日本エピジェネティクス研究会年会 

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    Event date: 2007.6

    Language:Japanese   Presentation type:Poster presentation  

    Venue:大阪大学吹田キャンパス・コンベンションセンター   Country:Japan  

  59. 染色体分離を制御する植物Auroraキナーゼ

    栗原大輔, 野田勝紀, 河邊昭, 藤本聡, 内山進, 松永幸大, 福井希一

    染色体学会2006年度(第57回)年会 

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    Event date: 2006.11

    Language:Japanese   Presentation type:Poster presentation  

    Venue:千葉大学けやき会館   Country:Japan  

  60. 細胞分裂期における植物Auroraキナーゼの機能解析

    栗原大輔, 野田勝紀, 河邊昭, 藤本聡, 内山進, 松永幸大, 福井希一

    「植物の生殖過程におけるゲノム障壁」ワークショップ 

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    Event date: 2006.11

    Language:Japanese   Presentation type:Poster presentation  

    Venue:国立遺伝学研究所   Country:Japan  

  61. 細胞分裂期における植物Auroraキナーゼの機能解析

    栗原大輔, 野田勝紀, 河邊昭, 藤本聡, 内山進, 松永幸大, 福井希一

    日本植物学会第70回大会 

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    Event date: 2006.9

    Language:Japanese   Presentation type:Poster presentation  

    Venue:熊本大学大学教育センター棟   Country:Japan  

  62. 細胞分裂期において植物Auroraキナーゼは染色体分離を制御する

    栗原大輔, 野田勝紀, 河邊昭, 藤本聡, 内山進, 松永幸大, 福井希一

    日本植物形態学会第18回総会・大会 

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    Event date: 2006.9

    Language:Japanese   Presentation type:Poster presentation  

    Venue:熊本大学大学教育センター棟   Country:Japan  

  63. Aurora kinases are required for chromosome segregation in plants International conference

    Daisuke Kurihara, Masanori Noda, Akira Kawabe, Satoru Fujimoto, Susumu Uchiyama, Sachihiro Matsunaga, Kiichi Fukui

    20th IUBMB Congress 

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    Event date: 2006.6

    Language:English   Presentation type:Poster presentation  

    Country:Japan  

  64. Auroraキナーゼ阻害剤は植物のヒストンH3リン酸化を抑制し細胞周期進行を阻害する

    栗原大輔, 野田勝紀, 河邊昭, 藤本聡, 内山進, 松永幸大, 福井希一

    日本分子生物学会第28回年会 

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    Event date: 2005.12

    Language:Japanese   Presentation type:Poster presentation  

    Venue:ヤフードーム(福岡県福岡市)   Country:Japan  

  65. 植物における分裂期特異的なヒストンH3のリン酸化

    栗原大輔, 松永幸大, 藤本聡, 内山進, 福井希一

    イネ・シロイヌナズナ合同ワークショップ 

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    Event date: 2005.7

    Language:Japanese   Presentation type:Poster presentation  

    Venue:奈良県文化会館   Country:Japan  

  66. 植物における分裂期特異的なヒストンH3のリン酸化

    栗原大輔, 内山進, 松永幸大, 福井希一

    第46回日本植物生理学会年会 

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    Event date: 2005.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:新潟コンベンションセンター 朱鷺メッセ   Country:Japan  

  67. In vitro における植物Aurora キナーゼとヒストンH3との相互作用

    栗原大輔, 河邊昭, 中川勝之, 内山進, 松永幸大, 福井希一

    第56回日本生物工学会大会  

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    Event date: 2004.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:名城大学天白キャンパス   Country:Japan  

  68. Interaction of plant Aurora kinases with histone H3 in vitro International conference

    Daisuke Kurihara, Akira Kawabe, Katsuyuki Nakagawa, Masanori Noda, Hiroaki Nakano, Susumu Uchiyama, Sachihiro Matsunaga, Kiichi Fukui

    The 2nd Asian Chromosome Colloquium 

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    Event date: 2004.5

    Language:English   Presentation type:Poster presentation  

    Country:Korea, Republic of  

  69. 受精卵の不等分裂を制御する細胞骨格ダイナミクス

    木全祐資, 檜垣匠, 河島友和, 河島友和, 栗原大輔, 栗原大輔, 佐藤良勝, 山田朋美, 山田朋美, 馳澤盛一郎, BERGER Frederic, 東山哲也, 東山哲也, 東山哲也, 植田美那子, 植田美那子

    日本植物学会大会研究発表記録  2017.9.1 

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  70. 時空間的制御に着目したRNA分解経路の比較解析

    元村一基, 丸山大輔, 栗原大輔, 熊倉直祐, 渡邊雄一郎, 東山哲也

    日本植物学会大会研究発表記録  2017.9.1 

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  71. 植物組織における透明化イメージング Invited

    栗原大輔

    レーザ顕微鏡研究会講演会抄録集  2018.1.19 

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  72. 植物胚発生における細胞間コミュニケーションによる細胞運命制御機構の解明

    栗原大輔, 大谷悠登, 石田喬志, 澤進一郎, 東山哲也

    日本植物学会第83回大会  2019.9.17 

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  73. 植物透明化試薬ClearSeeの改良

    栗原大輔, 水多陽子, 永原史織, 東山哲也

    日本植物学会第82回大会  2018.9.15 

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  74. 深部イメージングで探る植物生殖の謎

    水多陽子, 栗原大輔, 東山哲也

    日本植物学会第83回大会  2019.9.16 

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  75. 生殖段階における個々のRNA分解経路のイメージング解析

    元村一基, 丸山大輔, 栗原大輔, 熊倉直祐, 渡邊雄一郎, 東山哲也, 東山哲也

    日本植物生理学会年会(Web)  2018 

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  76. 花粉への一過的遺伝子導入系による花粉管及び精細胞の動態解析

    永原史織, 栗原大輔, 東山哲也, 水多陽子

    第60回日本植物生理学会年会  2019.3.14 

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  77. 花粉管をベクターとした生殖細胞の遺伝子改変と植物生殖機構の解明

    水多陽子, 永原史織, 栗原大輔, 東山哲也

    第60回日本植物生理学会年会  2019.3.13 

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  78. 雌性配偶体形成過程における細胞運命制御機構の解析

    栗原大輔, 須崎大地, 東山哲也, 東山哲也

    日本植物学会大会研究発表記録  2017.9.9 

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  79. 受精による細胞極性の破壊と再構成~ライブイメージングで迫る受精前後の細胞内変化~

    植田美那子, 木全祐資, 加藤壮英, 檜垣匠, 山田朋美, 栗原大輔, 森田(寺尾)美代, 馳澤盛一郎, 東山哲也, 田坂昌生

    日本植物学会大会研究発表記録  2017.9.1 

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  80. AS2 と協調的に働く核小体局在タンパク質 RNA HELICASE10 の AS2 body の局在における機能の解析

    安藤沙友里, 岩井雅斗, 小島晶子, 栗原大輔, 東山哲也, 町田泰則, 町田千代子

    第61回日本植物生理学会年会  2020.3.21 

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  81. Calcium dynamics of the pollen tube in ovular guidance International conference

    Kumi Matsuura-Tokita, Yoko Mizuta, Daisuke Kurihara, Tetsuya Higashiyama

    the 25th International Congress on Sexual Plant Reproduction  2018.6.13 

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  82. Imaging Analysis of mRNA decay mutants at early plant development International conference

    Kazuki Motomura, Daisuke Maruyama, Daisuke Kurihara, Naoyoshi Kumakura, Yuichiro Watanabe, Tetsuya Higashiyama

    the 25th International Congress on Sexual Plant Reproduction  2018.6.13 

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  83. Intracellular dynamics controlling Arabidopsis zygote polarization International conference

    Yusuke Kimata, Takehide Kato, Takumi Higaki, Daisuke Kurihara, Tomomi Yamada, Shoji Segami, Miyo Terao Morita, Masayoshi Maeshima, Seiichiro Hasezawa, Tetsuya Higashiyama, Masao Tasaka, Minako Ueda

    the 25th International Congress on Sexual Plant Reproduction  2018.6.12 

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  84. Live cell analysis and deep imaging for plant development Invited

    Daisuke Kurihara

    JSOL2019 (第16回日本ナス科コンソーシアム年会)  2019.9.26 

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  85. Live-cell analysis of female gametophyte development in Arabidopsis International conference

    Daisuke Kurihara, Daichi Susaki, Tetsuya Higashiyama

    the 25th International Congress on Sexual Plant Reproduction  2018.6.12 

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  86. Live-cell analysis of female gametophyte development in Arabidopsis International conference

    Daisuke Kurihara, Daichi Susaki, Tetsuya Higashiyama

    Synthetic Morphogenesis: From Gene Circuits to Tissue Architecture  2019.3.18 

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  87. Live-cell imaging of the axis formation in Arabidopsis zygote Invited International conference

    Minako Ueda, Yusuke Kimata, Takehide Kato, Takumi Higaki, Daisuke Kurihara, Tomomi Yamada, Shoji Segami, Miyo Terao Morita, Masayoshi Maeshima, Seiichiro Hasezawa, Tetsuya Higashiyama, Masao Tasaka, Naoe Ando

    the 25th International Congress on Sexual Plant Reproduction  2018.6.14 

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  88. Synthetic biological approach to understand plant gametic interactions by micromanipulation techniques International conference

    Kohdai Nakajima, Taeko Sasaki, Shiori Nagahara, Clari Valansi, Daisuke Kurihara, Yoshikatsu Sato, Narie Sasaki, Benjamin Podbilewicz, Tetsuya Higashiyama

    the 25th International Congress on Sexual Plant Reproduction  2018.6.12 

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  89. Two-photon imaging reveals spatio-temporal regulation on the one-to-one pollen tube guidance International conference

    Yoko Mizuta, Daisuke Kurihara, Shiori Nagahara, Tetsuya Higashiyama

    the 25th International Congress on Sexual Plant Reproduction  2018.6.12 

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  90. さらなる植物透明化へ向けて

    栗原大輔, 水多陽子, 永原史織, 東山哲也

    日本植物形態学会第30回大会  2018.9.13 

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  91. シロイヌナズナにおける初期胚のライブイメージング

    植田美那子, 木全祐資, 田中小百合, 加藤壮英, 桧垣匠, 栗原大輔, 山田朋美, 安藤奈央惠, 森田(寺尾)美代, 瀬上紹嗣, 前島正義, 馳澤盛一郎, 桑田啓子, 佐藤綾人, 鈴木孝征, 東山哲也, 田坂昌生

    第60回日本植物生理学会年会  2019.3.14 

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  92. シロイヌナズナ初期胚の細胞運命制御機構に関わる因子の探索

    栗原大輔, 大谷悠登, 石田喬志, 澤進一郎, 東山哲也

    日本植物形態学会第31回大会  2019.9.14 

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  93. シロイヌナズナ新規核膜融合因子Gex1の細胞内動態の解析

    鈴木 千晴, 矢部 あやか, 栗原 大輔, 佐藤 良勝, 東山 哲也, 西川 周一

    日本植物学会第83回大会  2019.9.17 

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  94. シロイヌナズナ胚のパターン形成におけるHD-ZIP IV転写因子群の機能の解析

    田中小百合, 栗原大輔, 柳沢直樹, 東山哲也, 植田美那子

    日本植物学会第82回大会  2018.9.15 

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  95. シロイヌナズナ花粉管誘引のライブイメージング

    時田公美, 水多陽子, 水多陽子, 栗原大輔, 東山哲也, 東山哲也

    日本植物学会大会研究発表記録  2017.9.1 

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  96. パーティクルボンバードメント法による植物生殖細胞へのCRISPR/Cas9の導入

    永原史織, 栗原大輔, 東山哲也, 水多陽子

    日本生化学会大会(Web)  2017 

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  97. ライブイメージングで迫るシロイヌナズナ受精卵の極性化機構 Invited

    植田美那子, 木全祐資, 田中小百合, 檜垣匠, 栗原大輔, 東山哲也

    日本植物学会第83回大会  2019.9.15 

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  98. 二光子イメージングによる一対一受精機構の解明

    水多陽子, 栗原大輔, 東山哲也

    日本植物形態学会第31回大会  2019.9.14 

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  99. 光ピンセットを用いた細胞操作による受精因子解析の試み

    中島耕大, 永原史織, 佐々木妙子, Clari Valansi, 栗原大輔, 佐藤良勝, 佐々木成江, Benjamin Podbilewicz, 東山哲也

    日本植物学会第82回大会  2018.9.15 

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  100. 光細胞操作技術を用いて,受精因子の機能を探る

    中島耕大, 永原史織, 佐々木妙子, Clari Valansi, 栗原大輔, 佐藤良勝, 佐々木成江, Benjamin Podbilewicz, 東山哲也

    日本植物形態学会第30回大会  2018.9.13 

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  101. 動物培養細胞への導入による動植物受精因子のライブ解析

    中島耕大, Clari Valansi, 栗原大輔, 佐々木成江, Benjamin Podbilewicz, 東山哲也

    日本植物形態学会第31回大会  2019.9.14 

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  102. 動物培養細胞を用いた動植物の受精因子群の機能解析

    中島耕大, Clari Valansi, 栗原大輔, 佐々木 成江, Benjamin Podbilewicz, 東山哲也

    日本植物学会第83回大会  2019.9.16 

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  103. 化合物スクリーニングにより見出された新規細胞分裂阻害剤 Invited

    木全祐資, 佐藤綾人, 桑田啓子, 鈴木孝征, 山田萌恵, 栗原大輔, 山田朋美, 東山哲也, 植田美那子

    日本植物学会第83回大会  2019.9.15 

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Research Project for Joint Research, Competitive Funding, etc. 2

  1. 植物雌性配偶体をモデルとした細胞運命制御機構の解明

    2022.4

    科学技術振興機構 (JST)  創発的研究支援事業 

    栗原大輔

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    Authorship:Principal investigator 

  2. 膜融合による植物への長鎖DNA導入技術の開発

    2018.10 - 2022.3

    科学技術振興機構(JST)  戦略的創造研究推進事業(さきがけ) 

    栗原大輔

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    Authorship:Principal investigator  Grant type:Competitive

    Grant amount:\39000000 ( Direct Cost: \39000000 、 Indirect Cost:\11700000 )

KAKENHI (Grants-in-Aid for Scientific Research) 9

  1. 両性花の可塑性を支える受精分子群の破壊と再構築

    Grant number:22H05178  2022.6 - 2027.3

    科学研究費助成事業  学術変革領域研究(A)

    奥田 哲弘, 越阪部 晃永, 栗原 大輔

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    多くの生物では、卵子と精子におけるリガンド-受容体の1対1関係により自他認識がなされ受精が成立する。しかしながら、着生した環境に制限される植物では、自他認識因子が目まぐるしく変遷していることが明らかになりつつある。本研究では、受精過程の自他認識リガンド-受容体群の新機能創出を担う分子間相互作用を解明すること、双子葉・単子葉植物間、さらに裸子植物とのゲノム横断的解析による受精因子の変遷過程の遡及をおこなう。これにより、受精過程における卵装置による、花粉管の誘引機構の進化的多様性を理解する。さらに、両性花獲得後も多様化してきた雌雄の分子間相互作用の場でもある花粉管誘引の分子実態解明を目指す。

  2. 植物初期胚発生におけるリガンド-受容体を介した胚性再獲得機構の解明

    Grant number:22H04668  2022.4 - 2024.3

    科学研究費助成事業  新学術領域研究(研究領域提案型)

    栗原 大輔

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    Grant amount:\8320000 ( Direct Cost: \6400000 、 Indirect Cost:\1920000 )

    本研究の目的は、我々が確立したin vitro胚発生系を利用し、植物受精胚の「全能性プログラム」制御に関わる因子を明らかにすることである。本研究では、ペプチドホルモンに着目して、リガンド--受容体シグナル経路を介して胚体外細胞が胚性再獲得する分子機構の解明を目指して研究を行い、計画研究班の哺乳動物研究との連携により、生命の根幹をなす仕組みに潜む普遍的なメカニズムの発見に繋げていきたい。

  3. 作物の生理障害の機構解明におけるブレークスルーテクノロジーの開発と検証

    Grant number:21H04721  2021.4 - 2026.3

    科学研究費助成事業  基盤研究(A)

    金山 喜則, 高橋 英樹, 渡部 敏裕, 須川 成利, 栗原 大輔, 黒田 理人

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    環境ストレスによって引き起こされる生理障害は、病虫害と並んで世界・日本で農業被害の双璧である。しかし原因が明確な病害虫と比べてその研究は遅れており、発生機構の全容解明は困難な状況にある。そこで本研究では、世界で最も生産されている野菜であるトマトに甚大な被害を及ぼす尻腐れ果をモデルケースとし、ブレークスルーテクノロジーとして原因イオンのレポータータンパク質によるイメージング、高感度センサ等による障害部位の早期検出や内部構造の可視化、イオンや遺伝子の網羅的解析と遺伝子発現制御によるエビデンスの獲得により尻腐れ果の発生機構を解明するとともに、生理障害の解析プラットフォームを提案する。

  4. 植物胚発生における胚性再獲得と全能性消失機構の解明

    Grant number:20H05358  2020.4 - 2022.3

    日本学術振興会  科学研究費助成事業  新学術領域研究(研究領域提案型)

    栗原 大輔

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    Authorship:Principal investigator 

    Grant amount:\7800000 ( Direct Cost: \6000000 、 Indirect Cost:\1800000 )

    本研究の目的は、我々が確立したin vitro胚発生系を利用し、植物受精胚の「全能性プログラム」制御に関わる因子を明らかにすることである。本研究では、[1]植物ホルモンであるオーキシンが全能性獲得に必要であるか、[2]全能性消失はいつ、どのように起こるのかという二点に着目して研究を行い、計画研究班の哺乳動物研究との連携により、生命の根幹をなす仕組みに潜む普遍的なメカニズムの発見に繋げていきたい。
    今年度は、オーキシンは胚発生における全能性再獲得に寄与するか?、および胚柄細胞における全能性消失時期特定について、オーキシン変異体を用いたレーザー照射実験による全能性再獲得解析を実施した。
    オーキシンは体細胞リプログラミング過程でも全能性獲得に重要な植物ホルモンであると知られているが、受精胚における全能性プログラムにどのように寄与しているかは未だ不明である。受精後、どの時期からオーキシンが受精胚に存在するかはまだ不明であるが、母体組織からのオーキシン供給も報告されているため、実験にはオーキシン生合成遺伝子変異体を用いた。
    また、これまで受精卵が不等分裂した一細胞期胚の頂端細胞を破壊したとき、胚柄細胞が細胞運命を転換することは明らかにしていたが、その後どの時期まで全能性再獲得があるかは不明である。そこで、オーキシン生合成遺伝子変異体を用いて、受精卵分裂直後の一細胞期胚において頂端細胞をレーザーで破壊して、その後の発生を二光子顕微鏡でライブイメージングを行った。
    その結果、オーキシン生合成変異体についても、野生型と同様に頂端細胞が破壊されたときには胚柄細胞の細胞運命転換が見られた。また、一細胞期胚よりも分裂が進んだ初期胚において、同様に胚体細胞を破壊したところ胚柄細胞の細胞運命転換が見られた。このことより、受精卵分裂直後だけではなく、分裂が進んだ時期でも全能性再獲得能があること、またオーキシンは全能性再獲得に関与していないことが示唆された。
    本年度の計画である、オーキシンは胚発生における全能性再獲得に寄与するか?、および胚柄細胞における全能性消失時期特定について、結果を得ることができた。
    オーキシンについては阻害剤を用いて、さらに解析を進める。また、より後期の胚を用いて解析を進め、全能性消失時期を特定し、全能性消失・全能性再獲得に重要な遺伝子群を同定する。

  5. Investigation of the relationship between positional information and cell identity by controlling the nuclear dynamics using optical manipulation

    Grant number:18K19331  2018.6 - 2020.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Challenging Research (Exploratory)

    Kurihara Daisuke

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    Authorship:Principal investigator 

    Grant amount:\6370000 ( Direct Cost: \4900000 、 Indirect Cost:\1470000 )

    In this study, we aimed to clarify the relationship between nuclear positional information and cell fate specification to elucidate the molecular mechanisms of cell fate specification in plants using a simple female gametophyte formation process with a small number of cells. We established the in vitro female gametophyte development system of Arabidopsis and monitored the dynamics of female gametophyte development in real-time. Analysis of the spatiotemporal information of nuclear dynamics and the expression of cell fate marker during the female gametophyte development suggested that the cell fate specification initiated earlier than previously thought. We also clarified the problem to be overcome in the intracellular organelle manipulation technology in the plant cells.

  6. Elucidation of the cell fate regulation by cell-cell communication in plant embryogenesis

    Grant number:17H03697  2017.4 - 2020.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

    Kurihara Daisuke

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    Grant amount:\17680000 ( Direct Cost: \13600000 、 Indirect Cost:\4080000 )

    Embryogenesis is a fundamental process in the construction of the complex structures and functions of higher organisms from a single cell, the zygote. The purpose of this study was to clarify the molecular entities of cell-cell communication involved in the regulation of cell fate during embryogenesis. We discovered a candidate mutant as a result of comprehensive analysis focusing on ligand-receptor pairs as signaling pathways that transport outside the cell. We collected data to identify and analyze the causative genes. We also tried to establish the optical manipulation technique for analyzing the signal pathway transported in the cell and clarified the problems and improvement points.

  7. 光顕微操作技術の開発を基盤とした植物初期胚発生における細胞分裂パターン機構の解析

    Grant number:23870014  2011.4 - 2013.3

    科学研究費補助金  研究活動スタート支援

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    Authorship:Principal investigator 

  8. 光操作技術の開発による植物胚発生におけるヒストンH3バリアントの機能解析

    Grant number:09J05807  2009.4 - 2010.12

    科学研究費補助金 

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    Authorship:Principal investigator 

    Grant amount:\1900000 ( Direct Cost: \1900000 )

  9. ヒストンH3リン酸化の可視化による染色体構造構築メカニズムの解明

    Grant number:06J09144  2006.4 - 2009.3

    科学研究費補助金 

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    Authorship:Principal investigator 

    Grant amount:\2800000 ( Direct Cost: \2800000 )

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Industrial property rights 3

  1. 植物細胞分裂抑制剤

    法人名大

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    Date applied:2016.2

    Announcement no:2017-145218  Date announced:2017.8

    Country of applicant:Domestic   Country of acquisition:Domestic

  2. CLEARSEE

    栗原大輔

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    Applicant:国立大学法人名古屋大学

    Application no:商願2016-006373  Date applied:2016.1

    Patent/Registration no:第5864936号  Date registered:2016.7 

    Country of applicant:Domestic  

  3. 植物組織透明化剤

    東海国立大学機構

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    Date applied:2015.12

    Announcement no:2017-108684  Date announced:2017.6

    Patent/Registration no:06601842 

    Country of applicant:Domestic   Country of acquisition:Domestic

 

Teaching Experience (Off-campus) 1

  1. Seminar in Bioscience B

    2020.8 Chubu University)