担当区分：筆頭著者,　責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1093/jmicro/dfw017

PubMed

担当区分：筆頭著者,　責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

Actin filaments are polar structures that exhibit a fast growing plus end and a slow growing minus end. According to their organization in cells, in parallel or antiparallel arrays, they can serve, respectively, in protrusions or in contractions. The determination of actin filament polarity in subcellular compartments is therefore required to establish their local function. Myosin binding has previously been the sole method of polarity determination. Here, we report the first direct determination of actin filament polarity in the cell without myosin binding. Negatively stained cytoskeletons of lamellipodia were analyzed by adapting electron tomography and a single particle analysis for filamentous complexes. The results of the stained cytoskeletons confirmed that all actin filament ends facing the cell membrane were the barbed ends. In general, this approach should be applicable to the analysis of actin polarity in tomograms of the actin cytoskeleton.

記述言語：英語   掲載種別：研究論文（学術雑誌）  

Using correlated live-cell imaging and electron tomography we found that actin branch junctions in protruding and treadmilling lamellipodia are not concentrated at the front as previously supposed, but link actin filament subsets in which there is a continuum of distances from a junction to the filament plus ends, for up to at least 1 mum. When branch sites were observed closely spaced on the same filament their separation was commonly a multiple of the actin helical repeat of 36 nm. Image averaging of branch junctions in the tomograms yielded a model for the in vivo branch at 2.9 nm resolution, which was comparable with that derived for the in vitro actin-Arp2/3 complex. Lamellipodium initiation was monitored in an intracellular wound-healing model and was found to involve branching from the sides of actin filaments oriented parallel to the plasmalemma. Many filament plus ends, presumably capped, terminated behind the lamellipodium tip and localized on the dorsal and ventral surfaces of the actin network. These findings reveal how branching events initiate and maintain a network of actin filaments of variable length, and provide the first structural model of the branch junction in vivo. A possible role of filament capping in generating the lamellipodium leaflet is discussed and a mathematical model of protrusion is also presented.

担当区分：筆頭著者,　最終著者,　責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

Actin is one of the most abundant proteins in eukaryote cells, which forms a double stranded filament. The actin filament is not only a main component of the cytoskeleton, but also acts as a motor protein which moves toward one specific end, the barbed end, driven by polymerization at the barbed end and depolymerization at the other end, the pointed end, without any associated proteins. This motor activity is referred to as "treadmilling" and it represents the simplest motor system known, consisting of only one 42 kDa protein, actin. Here we report the minimum requirements of the actin-like motor system elucidated by computer simulations: (1) Nucleotide binding and ATPase activity in the filament; (2) Polarity in the rates of polymerization and depolymerization between the two ends; and (3) The dependence of the subunit-subunit interactions on the bound nucleotide. These requirements are simple and this knowledge should facilitate the development of artificial molecular motor systems in the future.

担当区分：筆頭著者,　責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

The actin filament has clear polarity where one end, the pointed end, has a much slower polymerization and depolymerization rate than the other end, the barbed end. This intrinsic difference of the ends significantly affects all actin dynamics in the cell, which has central roles in a wide spectrum of cellular functions. The detailed mechanism underlying this difference has remained elusive, because high-resolution structures of the filament ends have not been available. Here, we present the structure of the actin filament pointed end obtained using a single particle analysis of cryo-electron micrographs. We determined that the terminal pointed end subunit is tilted towards the penultimate subunit, allowing specific and extra loop-to-loop inter-strand contacts between the two end subunits, which is not possible in other parts of the filament. These specific contacts prevent the end subunit from dissociating. For elongation, the loop-to-loop contacts also inhibit the incorporation of another actin monomer at the pointed end. These observations are likely to account for the less dynamic pointed end.

DOI： doi:10.1038/emboj.2011.48

記述言語：英語   掲載種別：研究論文（学術雑誌）  

Spire is an actin nucleator that initiates actin polymerization at a specific place in the cell. Similar to the Arp2/3 complex, spire was initially considered to bind to the pointed end of the actin filament when it generates a new actin filament. Subsequently, spire was reported to be associated with the barbed end (B-end); thus, there is still no consensus regarding the end with which spire interacts. Here, we report direct evidence that spire binds to the B-end of the actin filament, under conditions where spire accelerates actin polymerization. Using electron microscopy, we visualized the location of spire bound to the filament by gold nanoparticle labeling of the histidine-tagged spire, and the polarity of the actin filament was determined by image analysis. In addition, our results suggest that multiple spires, linked through one gold nanoparticle, enhance the acceleration of actin polymerization. The B-end binding of spire provides the basis for understanding its functional mechanism in the cell.

DOI： doi:10.1016/j.jmb.2010.12.045

担当区分：最終著者,　責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

A large number of actin-binding proteins (ABPs) regulate various kinds of cellular events in which the superstructure of the actin cytoskeleton is dynamically changed. Thus, to understand the actin dynamics in the cell, the mechanisms of actin regulation by ABPs must be elucidated. Moreover, it is particularly important to identify the side, barbed-end or pointed-end ABP binding sites on the actin filament. However, a simple, reliable method to determine the ABP binding sites on the actin filament is missing. Here, a novel electron microscopic method for determining the ABP binding sites is presented. This approach uses a gold nanoparticle that recognizes a histidine tag on an ABP and an image analysis procedure that can determine the polarity of the actin filament. This method will facilitate future study of ABPs.

DOI： 10.1016/j.jmb.2011.01.054

記述言語：英語   掲載種別：研究論文（学術雑誌）  

Actin plays crucial parts in cell motility through a dynamic process driven by polymerization and depolymerization, that is, the globular (G) to fibrous (F) actin transition. Although our knowledge about the actin-based cellular functions and the molecules that regulate the G- to F-actin transition is growing, the structural aspects of the transition remain enigmatic. We created a model of F-actin using X-ray fibre diffraction intensities obtained from well oriented sols of rabbit skeletal muscle F-actin to 3.3 A in the radial direction and 5.6 A along the equator. Here we show that the G- to F-actin conformational transition is a simple relative rotation of the two major domains by about 20 degrees. As a result of the domain rotation, the actin molecule in the filament is flat. The flat form is essential for the formation of stable, helical F-actin. Our F-actin structure model provides the basis for understanding actin polymerization as well as its molecular interactions with actin-binding proteins.

記述言語：英語   掲載種別：研究論文（学術雑誌）  

ParM is a prokaryotic actin homologue, which ensures even plasmid segregation before bacterial cell division. In vivo, ParM forms a labile filament bundle that is reminiscent of the more complex spindle formed by microtubules partitioning chromosomes in eukaryotic cells. However, little is known about the underlying structural mechanism of DNA segregation by ParM filaments and the accompanying dynamic instability. Our biochemical, TIRF microscopy and high-pressure SAX observations indicate that polymerization and disintegration of ParM filaments is driven by GTP rather than ATP and that ParM acts as a GTP-driven molecular switch similar to a G protein. Image analysis of electron micrographs reveals that the ParM filament is a left-handed helix, opposed to the right-handed actin polymer. Nevertheless, the intersubunit contacts are similar to those of actin. Our atomic model of the ParM-GMPPNP filament, which also fits well to X-ray fibre diffraction patterns from oriented gels, can explain why after nucleotide release, large conformational changes of the protomer lead to a breakage of intra- and interstrand interactions, and thus to the observed disintegration of the ParM filament after DNA segregation.

記述言語：英語   掲載種別：研究論文（学術雑誌）  

Cytoplasmic dynein is a large, microtubule-dependent molecular motor (1.2 MDa). Although the structure of dynein by itself has been characterized, its conformation in complex with microtubules is still unknown. Here, we used cryoelectron microscopy (cryo-EM) to visualize the interaction between dynein and microtubules. Most dynein molecules in the nucleotide-free state are bound to the microtubule in a defined conformation and orientation. A 3D image reconstruction revealed that dynein's head domain, formed by a ring-like arrangement of AAA+ domains, is located approximately 280 A away from the center of the microtubule. The order of the AAA+ domains in the ring was determined by using recombinant markers. Furthermore, a 3D helical image reconstruction of microtubules with a dynein's microtubule binding domain [dynein stalk (DS)] revealed that the stalk extends perpendicular to the microtubule. By combining the 3D maps of the dynein-microtubule and DS-microtubule complexes, we present a model for how dynein in the nucleotide-free state binds to microtubules and discuss models for dynein's power stroke.

担当区分：筆頭著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

Dynein is a minus-end-directed microtubule (MT) motor that is responsible for the wide range of MT-based motility in eukaryotic cells. Detailed mechanism of the dynein chemomechanical conversion is still unknown, partly because the structure of dynein is not studied at high resolution. To address this problem and reconstruct the dynein-MT complex at higher resolution, we have developed new procedures based on single particle analysis. To accurately determine the orientation of the dynein-MT complex, we introduced a "dynein track model" to restrict the possible dynein positions on the images. We tested our procedures by reconstructing structures from simulated dynein-MT complex images. Starting from the simulated noisy images generated using three different models of the dynein-MT complex, we have successfully recovered the original three-dimensional (3-D) structure. We also showed that our procedure is robust against fluctuation of the dynein molecules and can determine the structure even when the dynein position fluctuates to a certain extent. Convergence of the final 3-D structure can be tested with a "two-dimensional (2-D) agreement value," which we introduced to see whether the final structure is a result of overfit from fluctuating dynein or not. When the procedures did not work well due to the fluctuation, we could recognize the failure by this 2-D agreement value. Finally, the actual structure of the dynein-MT complex was determined from actual cryoelectron micrographs of Dictyostelium cytoplasmic dynein-MT complex. This method has revealed the detailed 3-D structures of the dynein-MT complex and will shed light on the motor mechanism of the dynein molecule.

担当区分：筆頭著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

In eukaryotic cells, actin filaments play various crucial roles by altering their spatial and temporal distributions in the cell. The distribution of actin filaments is regulated by the binding of end-binding proteins, including capping protein (CapZ in muscle), the Arp2/3 complex, gelsolin, formin and tropomodulin, to the end of the actin filament. In order to determine the nature of these regulations, structural elucidations of actin filament-end-binding protein complexes are crucially important. Here, we have developed new procedures on the basis of single-particle analysis to determine the structure of the end of actin filaments from electron micrographs. In these procedures, the polarity of the actin filament image, as well as the azimuth orientation and the axial position of each actin protomer within a short stretch near the filament end, were determined accurately. This improved both the stability and accuracy of the structural determination dramatically. We tested our procedures by reconstructing structures from simulated filament images, which were obtained from 24 model structures for the actin-CapZ complex. These model structures were generated by random docking of the atomic structure of CapZ to the barbed end of an atomic model of the actin filament. Of the 24 model structures, 23 were recovered correctly by the present procedures. We found that our analysis was robust against local aberrations of the helical twist near the end of the actin filament. Finally, the procedures were applied successfully to determine the structure of the actin-CapZ complex from real cryo-electron micrographs of the complex. This is the first method for elucidating the detailed 3D structures at the end of the actin filament.

担当区分：筆頭著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

The intracellular distribution and migration of many protein complexes and organelles is regulated by the dynamics of the actin filament. Many actin filament end-binding proteins play crucial roles in actin dynamics, since polymerization and depolymerization of actin protomers occur only at the filament ends. We present here an EM structure of the complex of the actin filament and hetero-dimeric capping protein (CP) bound to the barbed-end at 23 A resolution, by applying a newly developed methods of image analysis to cryo-electron micrographs. This structure was fitted by the crystal structure of CP and the proposed actin filament structure, allowing us to construct a model that depicts two major binding regions between CP and the barbed-end. This binding scheme accounted for the results of newly performed and previously published mutation experiments, and led us to propose a two-step binding model. This is the first determination of an actin filament end structure.

担当区分：筆頭著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

Muscle contraction is regulated by the intracellular Ca(2+ )concentration. In vertebrate striated muscle, troponin and tropomyosin on actin filaments comprise a Ca(2+)-sensitive switch that controls contraction. Ca(2+ )binds to troponin and triggers a series of changes in actin-containing filaments that lead to cyclic interactions with myosin that generate contraction. However, the precise location of troponin relative to actin and tropomyosin and how its structure changes with Ca(2+ )have been not determined. To understand the regulatory mechanism, we visualized the location of troponin by determining the three-dimensional structure of thin filaments from electron cryo-micrographs without imposing helical symmetry to approximately 35 A resolution. With Ca(2+), the globular domain of troponin was gourd-shaped and was located over the inner domain of actin. Without Ca(2+), the main body of troponin was shifted by approximately 30 A towards the outer domain and bifurcated, with a horizontal branch (troponin arm) covering the N and C-terminal regions of actin. The C-terminal one-third of tropomyosin shifted towards the outer domain of actin by approximately 35 A supporting the steric blocking model, however it is surprising that the N-terminal half of tropomyosin shifted less than approximately 12 A. Therefore tropomyosin shifted differentially without Ca(2+). With Ca(2+), tropomyosin was located entirely over the inner domain thereby allowing greater access of myosin for force generation. The interpretation of three-dimensional maps was facilitated by determining the three-dimensional positions of fluorophores labelled on specific sites of troponin or tropomyosin by applying probabilistic distance geometry to data from fluorescence resonance energy transfer measurements.

記述言語：英語  

DOI： 10.1016/j.jmb.2023.168295

Web of Science

PubMed

記述言語：英語  

DOI： 10.3389/fcell.2023.1105460

Web of Science

PubMed

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： https://doi.org/10.1098/rsob.220083

記述言語：英語  

DOI： 10.1098/rsob.220083

Web of Science

PubMed

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1126/sciadv.abm2225

Web of Science

PubMed

担当区分：筆頭著者,　最終著者,　責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： https://doi.org/10.33611/trs.2022-008

記述言語：英語   掲載種別：研究論文（学術雑誌）  

記述言語：英語  

DOI： 10.1021/acs.biomac.1c00533

Web of Science

PubMed

記述言語：英語   掲載種別：研究論文（学術雑誌）  

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： https://doi.org/10.1016/j.jmb.2021.166891

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1107/S2053230X20016829

Web of Science

PubMed

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1093/jmicro/dfaa028

Web of Science

PubMed

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1016/j.abb.2020.108446

Web of Science

PubMed

担当区分：筆頭著者,　最終著者,　責任著者   記述言語：日本語   掲載種別：研究論文（学術雑誌）  

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.3390/biom10050736

Web of Science

PubMed

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1371/journal.pgen.1008664

Web of Science

PubMed

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： https://doi.org/10.1007/s10974-019-09571-5

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1016/j.jmb.2019.05.047

Web of Science

PubMed

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1016/j.jmb.2019.05.048

Web of Science

PubMed

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1038/s41467-019-10779-9

Web of Science

PubMed

担当区分：筆頭著者,　責任著者   記述言語：日本語   掲載種別：研究論文（学術雑誌）  

DOI： doi:10.14952/SEIKAGAKU.2019.910109

記述言語：英語  

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1002/1873-3468.13218

Web of Science

担当区分：筆頭著者,　最終著者,　責任著者   記述言語：日本語  

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1002/bies.201700213

Web of Science

記述言語：英語   掲載種別：研究論文（国際会議プロシーディングス）  

Web of Science

担当区分：最終著者,　責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1002/cm.21384

Web of Science

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1038/s41598-017-06942-1

PubMed

記述言語：英語   掲載種別：研究論文（学術雑誌）  

Web of Science

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1093/jmicro/dfw037

PubMed

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1080/19420889.2016.1242538

PubMed

担当区分：筆頭著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1038/srep27472

PubMed

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1016/j.ab.2015.12.021

PubMed

担当区分：筆頭著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1073/pnas.1600129113

PubMed

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： doi/10.1371/journal.pbio.1001765

記述言語：英語   掲載種別：研究論文（学術雑誌）  

担当区分：筆頭著者   記述言語：日本語  

近年電子線トモグラフィーにより細胞内構造の可視化が急速に進展している一方で、細胞内蛋白質構造のような微細構造の解析は未だ難しい。主な原因は、１：細胞内構造のS/Nの悪さ、２：ミッシングエッジやミッシングピラミッドなどの、データが存在しない逆空間領域の取り扱いの二つである。筆者らは、細胞の中で膜構造に次いで顕著な構造物である線維状蛋白質構造、特にアクチンフィラメント網構造の解析に的を絞り、共同研究者のJohn Victor Smallらが１の問題を負染色法によって部分的に解決、筆者らが２の問題を回避する方策を考案することによって、ある程度有用な細胞内構造解析技術を構築することができた。本項ではその技術を概説する。

記述言語：英語   掲載種別：研究論文（学術雑誌）  

記述言語：英語   掲載種別：研究論文（学術雑誌）  

In preparation for mammalian cell division, microtubules repeatedly probe the cytoplasm to capture chromosomes and assemble the mitotic spindle. Critical features of this microtubule system are the formation of radial arrays centered at the centrosomes and dynamic instability, leading to persistent cycles of polymerization and depolymerization. Here, we show that actin homolog, ParM-R1 that drives segregation of the R1 multidrug resistance plasmid from Escherichia coli, can also self-organize in vitro into asters, which resemble astral microtubules. ParM-R1 asters grow from centrosome-like structures consisting of interconnected nodes related by a pseudo 8-fold symmetry. In addition, we show that ParM-R1 is able to perform persistent microtubule-like oscillations of assembly and disassembly. In vitro, a whole population of ParM-R1 filaments is synchronized between phases of growth and shrinkage, leading to prolonged synchronous oscillations even at physiological ParM-R1 concentrations. These results imply that the selection pressure to reliably segregate DNA during cell division has led to common mechanisms within diverse segregation machineries.

記述言語：英語   掲載種別：研究論文（学術雑誌）  

Eukaryotic F-actin is constructed from two protofilaments that gently wind around each other to form a helical polymer. Several bacterial actin-like proteins (Alps) are also known to form F-actin-like helical arrangements from two protofilaments, yet with varied helical geometries. Here, we report a unique filament architecture of Alp12 from Clostridium tetani that is constructed from four protofilaments. Through fitting of an Alp12 monomer homology model into the electron microscopy data, the filament was determined to be constructed from two antiparallel strands, each composed of two parallel protofilaments. These four protofilaments form an open helical cylinder separated by a wide cleft. The molecular interactions within single protofilaments are similar to F-actin, yet interactions between protofilaments differ from those in F-actin. The filament structure and assembly and disassembly kinetics suggest Alp12 to be a dynamically unstable force-generating motor involved in segregating the pE88 plasmid, which encodes the lethal tetanus toxin, and thus a potential target for drug design. Alp12 can be repeatedly cycled between states of polymerization and dissociation, making it a novel candidate for incorporation into fuel-propelled nanobiopolymer machines.

担当区分：筆頭著者,　最終著者,　責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

Actin forms a double-stranded filament, and the majority of actin filaments in the cell undergo the dynamic process of polymerization and depolymerization at both ends. Actin dynamics plays numerous important roles in eukaryotic cells. In order to understand actin dynamics, structural elucidation of the actin filament ends is particularly important because polymerization and depolymerization occurs only at the ends. We have developed original image analysis procedures to determine the structures of the actin filament ends from cryo-electron micrographs, and two structures have been determined. The structures revealed that the actin filament takes advantage of its double-stranded form to regulate its dynamics at both ends by a surprisingly simple mechanism.

記述言語：英語   掲載種別：研究論文（学術雑誌）  

The actin capping protein (CP) tightly binds to the barbed end of actin filaments, thus playing a key role in actin-based lamellipodial dynamics. V-1 and CARMIL proteins directly bind to CP and inhibit the filament capping activity of CP. V-1 completely inhibits CP from interacting with the barbed end, whereas CARMIL proteins act on the barbed end-bound CP and facilitate its dissociation from the filament (called uncapping activity). Previous studies have revealed the striking functional differences between the two regulators. However, the molecular mechanisms describing how these proteins inhibit CP remains poorly understood. Here we present the crystal structures of CP complexed with V-1 and with peptides derived from the CP-binding motif of CARMIL proteins (CARMIL, CD2AP, and CKIP-1). V-1 directly interacts with the primary actin binding surface of CP, the C-terminal region of the alpha-subunit. Unexpectedly, the structures clearly revealed the conformational flexibility of CP, which can be attributed to a twisting movement between the two domains. CARMIL peptides in an extended conformation interact simultaneously with the two CP domains. In contrast to V-1, the peptides do not directly compete with the barbed end for the binding surface on CP. Biochemical assays revealed that the peptides suppress the interaction between CP and V-1, despite the two inhibitors not competing for the same binding site on CP. Furthermore, a computational analysis using the elastic network model indicates that the interaction of the peptides alters the intrinsic fluctuations of CP. Our results demonstrate that V-1 completely sequesters CP from the barbed end by simple steric hindrance. By contrast, CARMIL proteins allosterically inhibit CP, which appears to be a prerequisite for the uncapping activity. Our data suggest that CARMIL proteins down-regulate CP by affecting its conformational dynamics. This conceptually new mechanism of CP inhibition provides a structural basis for the regulation of the barbed end elongation in cells.

記述言語：英語   掲載種別：研究論文（学術雑誌）  

In vivo fluorescence microscopy studies of bacterial cells have shown that the bacterial shape-determining protein and actin homolog, MreB, forms cable-like structures that spiral around the periphery of the cell. The molecular structure of these cables has yet to be established. Here we show by electron microscopy that Thermatoga maritime MreB forms complex, several mum long multilayered sheets consisting of diagonally interwoven filaments in the presence of either ATP or GTP. This architecture, in agreement with recent rheological measurements on MreB cables, may have superior mechanical properties and could be an important feature for maintaining bacterial cell shape. MreB polymers within the sheets appear to be single-stranded helical filaments rather than the linear protofilaments found in the MreB crystal structure. Sheet assembly occurs over a wide range of pH, ionic strength, and temperature. Polymerization kinetics are consistent with a cooperative assembly mechanism requiring only two steps: monomer activation followed by elongation. Steady-state TIRF microscopy studies of MreB suggest filament treadmilling while high pressure small angle x-ray scattering measurements indicate that the stability of MreB polymers is similar to that of F-actin filaments. In the presence of ADP or GDP, long, thin cables formed in which MreB was arranged in parallel as linear protofilaments. This suggests that the bacterial cell may exploit various nucleotides to generate different filament structures within cables for specific MreB-based functions.

記述言語：英語   掲載種別：研究論文（学術雑誌）  

Tuberculosis causes the most death in humans by any bacterium. Drug targeting of bacterial cytoskeletal proteins requires detailed knowledge of the various filamentous suprastructures and dynamic properties. Here, we have investigated by high resolution electron microscopy the assembly of cell division protein and microtubule homolog FtsZ from Mycobacterium tuberculosis (MtbFtsZ) in vitro in the presence of various monovalent salts, crowding agents and polycations. Supramolecular structures, including two-dimensional rings, three-dimensional toroids, and multistranded helices formed in the presence of molecular crowding, were similar to those observed by fluorescence microscopy in bacteria in vivo. Dynamic properties of MtbFtsZ filaments were visualized by light scattering and real time total internal reflection fluorescence microscopy. Interestingly, MtbFtsZ revealed a form of dynamic instability at steady state. Cation-induced condensation phenomena of bacterial cytomotive polymers have not been investigated in any detail, although it is known that many bacteria can contain high amounts of polycations, which may modulate the prokaryotic cytoskeleton. We find that above a threshold concentration of polycations which varied with the valence of the cation, ionic strength, and pH, MtbFtsZ mainly formed sheets. The general features of these cation-induced condensation phenomena could be explained in the framework of the Manning condensation theory. Chirality and packing defects limited the dimensions of sheets and toroids at steady state as predicted by theoretical models. In first approximation simple physical principles seem to govern the formation of MtbFtsZ suprastructures.

記述言語：英語   掲載種別：研究論文（学術雑誌）  

AlfA is a recently discovered DNA segregation protein from Bacillus subtilis that is distantly related to actin and the bacterial actin homologues ParM and MreB. Here we show that AlfA mostly forms helical 7/3 filaments, with a repeat of about 180 A, that are arranged in three-dimensional bundles. Other polymorphic structures in the form of two-dimensional rafts or paracrystalline nets were also observed. Here AlfA adopted a 16/7 helical symmetry, with a repeat of about 387 A. Thin polymers consisting of several intertwining filaments also formed. Observed helical symmetries of AlfA filaments differed from those of other members of the actin family: F-actin, ParM, or MreB. Both ATP and guanosine 5'-triphosphate are able to promote rapid AlfA filament formation with almost equal efficiencies. The helical structure is only preserved under physiological salt concentrations and at a pH between 6.4 and 7.4, the physiological range of the cytoplasm of B. subtilis. Polymerization kinetics are extremely rapid and compatible with a cooperative assembly mechanism requiring only two steps: monomer activation followed by elongation, making AlfA one of the most efficient polymerizing motors within the actin family. Phosphate release lags behind polymerization, and time-lapse total internal reflection fluorescence images of AlfA bundles are consistent with treadmilling rather than dynamic microtubule-like instability. High-pressure small angle X-ray scattering experiments reveal that the stability of AlfA filaments is intermediate between the stability of ParM and the stability of F-actin. These results emphasize that actin-like polymerizing machineries have diverged to produce a variety of filament geometries with diverse properties that are tailored for specific biological processes.

記述言語：英語   掲載種別：研究論文（学術雑誌）  

記述言語：英語   掲載種別：研究論文（学術雑誌）  

The actin homolog ParM plays a microtubule-like role in segregating DNA prior to bacterial cell division. Fluorescence and cryo-electron microscopy have shown that ParM forms filament bundles between separating DNA plasmids in vivo. Given the lack of ParM bundling proteins it remains unknown how ParM bundles form at the molecular level. Here we show using time-lapse TIRF microscopy, under in vitro molecular crowding conditions, that ParM-bundle formation consists of two distinct phases. At the onset of polymerization bundle thickness and shape are determined in the form of nuclei of short helically disordered filaments arranged in a liquid-like lattice. These nuclei then undergo an elongation phase whereby they rapidly increase in length. At steady state, ParM bundles fuse into one single large aggregate. This behavior had been predicted by theory but has not been observed for any other cytomotive biopolymer, including F-actin. We employed electron micrographs of ParM rafts, which are 2-D analogs of 3-D bundles, to identify the main molecular interfilament contacts within these suprastructures. The interface between filaments is similar for both parallel and anti-parallel orientations and the distribution of filament polarity is random within a bundle. We suggest that the interfilament interactions are not due to the interactions of specific residues but rather to long-range, counter ion mediated, electrostatic attractive forces. A randomly oriented bundle ensures that the assembly is rigid and that DNA may be captured with equal efficiency at both ends of the bundle via the ParR binding protein.

担当区分：筆頭著者   記述言語：日本語  

アクチンフィラメントや微小管などの繊維状タンパ
ク質重合体は，真核生物における細胞骨格，細胞運
動，接着，物質のとりこみ，排出，細胞分裂など非常
に多岐にわたる現象において中心的な存在である．そ
の時間的，空間的分布および機能は数多くの結合タン
パク質によって厳密に制御されており，この制御のあ
り方を知るためには，重合体に結合タンパク質が結合
した状態での3 次元構造を知らなくてはならない．し
かしながら，構造解析が難しいため，繊維状タンパク
質重合体の機能および制御機構はわかっていないこと
が多い．
私たちはこの状況を打破するために，繊維状構造を
決定するための独自の単粒子解析法の開発を進めてき
た．最近この手法は軌道に乗り，さまざまな構造解析
に適用できるようになった．本稿ではこの私たち独自
の単粒子解析法を，その利点と問題点を含めて概説し
たい

記述言語：英語   掲載種別：研究論文（学術雑誌）  

ParM, an actin homolog, forms left-handed two-start helical filaments that segregate DNA in bacteria prior to cell division. Our recent atomic model obtained from electron microscopy (EM) reconstructions of negatively stained ParM filaments implied that two salt bridges (Glu35-Lys258 and Asp63-Arg262) may be key inter-filament contacts that stabilize the left-handed ParM helix. We made mutations of these amino acids and probed the inter-strand interface of our model experimentally by EM and X-ray fiber diffraction. We found that several mutations, such as ParM single mutants Asp258 and Asp262 and double mutant Asp258/Arg262, were incapable of forming straight filaments in aqueous buffers and appeared ragged and unstructured. However, in the presence of crowding agents, straight filaments or filament bundles formed, which allowed us to elucidate the structure of these mutant filaments. Centrifugation of filaments also resulted in a pellet of straightened filaments that could be oriented in glass capillaries and gave detailed X-ray diffraction patterns. Both EM and X-ray diffraction showed that filaments formed from these ParM mutants were not double-stranded helical filaments but single protofilaments, indicating that these residues are important for formation of the ParM helix. Our data also confirm a major prediction of crowding theory, namely that molecular crowding shifts the equilibrium of even severely impaired, unstructured cytoskeletal polymers toward their structured native and functional state. ParM is the first example of a helical actin homolog that can be induced to form protofilaments.

記述言語：英語   掲載種別：研究論文（学術雑誌）  

In vivo cell division protein FtsZ from E. coli forms rings and spirals which have only been observed by low resolution light microscopy. We show that these suprastructures are likely formed by molecular crowding which is a predominant factor in prokaryotic cells and enhances the weak lateral bonds between proto-filaments. Although FtsZ assembles into single proto-filaments in dilute aqueous buffer, with crowding agents above a critical concentration, it forms polymorphic supramolecular structures including rings and toroids (with multiple protofilaments) about 200 nm in diameter, similar in appearance to DNA toroids, and helices with pitches of several hundred nm as well as long, linear bundles. Helices resemble those observed in vivo, whereas the rings and toroids may represent a novel energy minimized state of FtsZ, at a later stage of Z-ring constriction. We shed light on the molecular arrangement of FtsZ filaments within these suprastructures using high resolution electron microscopy.

担当区分：筆頭著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

The actin filament is quite dynamic in the cell. To determine the relationship between the structure and the dynamic properties of the actin filament, experiments using actin mutants are indispensable. We focused on Gln(137) to understand the relationships between two activities: the conformational changes relevant to the G- to F-actin transition and the activation of actin ATPase upon actin polymerization. To elucidate the function of Gln(137) in these activities, we characterized Gln(137) mutants of human cardiac muscle alpha-actin. Although all of the single mutants, Q137E, Q137K, Q137P, and Q137A, as well as the wild type were expressed by a baculovirus-based system, only Q137A and the wild type were purified to high homogeneity. The CD spectrum of Q137A was similar to that of the wild type, and Q137A showed the typical morphology of negatively stained Q137A F-actin images. However, Q137A had an extremely low critical concentration for polymerization. Furthermore, we found that Q137A polymerized 4-fold faster, cleaved the gamma-phosphate group of bound ATP 4-fold slower, and depolymerized 5-fold slower, as compared with the wild-type rates. These results suggest that Gln(137) plays dual roles in actin polymerization, in both the conformational transition of the actin molecule and the mechanism of ATP hydrolysis.

担当区分：筆頭著者   記述言語：日本語  

細胞運動や細胞分裂、細胞骨格など生命現象の根幹にかかわる機能を担うアクチン。
その機能には、アクチンフィラメントの端でおこる重合、脱落が重要な役割を果たしているが、詳しいメカニズムは不明であった。アクチンフィラメント端とアクチンフィラメント端結合タンパク質CPの３次元構造の決定によって、謎の一端が明かされた。

記述言語：英語   掲載種別：研究論文（学術雑誌）  

Using in vitro TIRF- and electron-microscopy, we reinvestigated the dynamics of native ParM, a prokaryotic DNA segregation protein and actin homolog. In contrast to a previous study, which used a cysteine ParM mutant, we find that the polymerization process of wild type ATP-ParM filaments consists of a polymerization phase and a subsequent steady state phase, which is dynamically unstable, like that of microtubules. We find that the apparent bidirectional polymerization of ParM, is not due to the intrinsic nature of this filament, but results from ParM forming randomly oriented bundles in the presence of crowding agents. Our results imply, that in the bacterium, ParM filaments spontaneously form bipolar bundles. Due to their intrinsic dynamic instability, ParM bundles can efficiently "search" the cytoplasmic lumen for DNA, bind it equally well at the bipolar ends and segregate it approximately symmetrically, by the insertion of ParM subunits at either end.

記述言語：英語   掲載種別：研究論文（学術雑誌）  

The dynactin complex interacts with dynein and numerous other proteins to provide for a wide range of subcellular transport functions. A detailed understanding of the structure and subunit organization of dynactin should yield new insights into its function. In the present study, we used single particle analysis to obtain a two-dimensional averaged image of dynactin isolated from chick embryo brains and visualized by negative stain electron microscopy (EM). Each individual image, consisting of the shoulder/sidearm and the rod, closely resembled the previously published quick-freeze deep-etch rotary-shadow electron micrographs. However, the averaged image revealed novel structural features that may have functional significance. The bulky shoulder complex has a triangular shape and is 13 nm wide and 8 nm high. The rod, with an overall length of 40 nm, consists of clearly defined lobes that are apparently grouped into three parts, the pointed-end complex, the middle segment, and the extra lobes at the barbed end. The pointed-end complex reveals the characteristic protrusions and clefts that were previously observed only in the isolated pointed-end complex. In the middle segment, the seven lobes are fitted to the helical symmetry of F-actin. A narrow but prominent gap separates the previously unidentified extra three lobes at the barbed end from the middle segment. The averaged image we obtained contrasts dramatically with the simple Arp1 polymer that was previously reported by single particle analysis of bovine brain dynactin. These apparent structural differences are probably due to the greater stability and integrity of the chick embryo brain dynactin preparation. We propose a new structural model for dynactin, based on our observations.

開催年月日： 2021年9月

記述言語：日本語   会議種別：シンポジウム・ワークショップ パネル（指名）  

開催地：オンライン  

開催年月日： 2020年12月

記述言語：日本語   会議種別：シンポジウム・ワークショップ パネル（指名）  

開催地：オンライン  

開催年月日： 2020年6月

記述言語：日本語   会議種別：シンポジウム・ワークショップ パネル（指名）  

開催地：オンライン  

開催年月日： 2020年2月

記述言語：日本語   会議種別：シンポジウム・ワークショップ パネル（指名）  

開催地：ウインクあいち   国名：日本国  

開催年月日： 2019年12月

記述言語：英語   会議種別：シンポジウム・ワークショップ パネル（指名）  

国名：日本国  

開催年月日： 2019年9月

記述言語：英語   会議種別：口頭発表（招待・特別）  

開催地：Bristol Univ. England   国名：グレートブリテン・北アイルランド連合王国(英国)  

開催年月日： 2019年6月

記述言語：日本語   会議種別：口頭発表（招待・特別）  

国名：日本国  

開催年月日： 2019年3月

記述言語：日本語   会議種別：シンポジウム・ワークショップ パネル（指名）  

開催地：朱鷺メッセ　新潟   国名：日本国  

開催年月日： 2019年3月

記述言語：日本語   会議種別：シンポジウム・ワークショップ パネル（指名）  

開催地：朱鷺メッセ、新潟   国名：日本国  

開催年月日： 2019年3月

記述言語：日本語   会議種別：シンポジウム・ワークショップ パネル（指名）  

開催地：岐阜大学   国名：日本国  

開催年月日： 2019年1月

記述言語：日本語   会議種別：シンポジウム・ワークショップ パネル（指名）  

開催地：千里ライフサイエンスセンター   国名：日本国  

開催年月日： 2018年9月

記述言語：日本語   会議種別：シンポジウム・ワークショップ パネル（公募）  

開催地：名古屋国際会議場   国名：日本国  

開催年月日： 2018年9月

国名：日本国  

アクチンを中心とした生理学

pythonをつかったプログラミング実習

pythonを使ったプログラミング実習

情報文化むけ生物学概論。

アクチンを中心にした生理学

pythonを用いたプログラミング実習

本講義では、「生物における情報・エネルギー変換機構」を分子生理学的に理解することを目的とする。生物は、外的環境を素早く感知し、適切に情報処理することで環境中を生き抜いている。ここでは、[1] 光受容分子による外部シグナル認識・処理機構、[2] MAPキナーゼによる細胞内シグナル伝達機構、[3] アクチン細胞骨格のエネルギー変換による行動制御機構、に着目する。近年、長らく謎であった分子レベルでの情報・エネルギー変換機構が解明されつつある。講義では分子の発見から分子機構の解明に至るさまざまな研究を紹介する。そこでは、生理学的方法による現象の解析、分子遺伝学的解析による原因分子の同定、生化学的解析による変換機構の解明、生物物理学的手法による計測、構造生物学的手法を用いた可視化などを紹介し、生命現象の理解に向けた様々な方法を理解することを目指す。これらについて、小テストで理解度を深めるとともに期末試験を行う。講義途中・講義後の発言・質問も歓迎する。全１２回のうち、5回を担当

pythonを用いたプログラミング実習

本講義では、「生物における情報・エネルギー変換機構」を分子生理学的に理解することを目的とする。生物は、外的環境を素早く感知し、適切に情報処理することで環境中を生き抜いている。ここでは、[1] 光受容分子による外部シグナル認識・処理機構、[2] MAPキナーゼによる細胞内シグナル伝達機構、[3] アクチン細胞骨格のエネルギー変換による行動制御機構、に着目する。近年、長らく謎であった分子レベルでの情報・エネルギー変換機構が解明されつつある。講義では分子の発見から分子機構の解明に至るさまざまな研究を紹介する。そこでは、生理学的方法による現象の解析、分子遺伝学的解析による原因分子の同定、生化学的解析による変換機構の解明、生物物理学的手法による計測、構造生物学的手法を用いた可視化などを紹介し、生命現象の理解に向けた様々な方法を理解することを目指す。これらについて、小テストで理解度を深めるとともに期末試験を行う。講義途中・講義後の発言・質問も歓迎する。全１２回のうち、5回を担当

本講義では、「生物における情報・エネルギー変換機構」を分子生理学的に理解することを目的とする。生物は、外的環境を素早く感知し、適切に情報処理することで環境中を生き抜いている。ここでは、[1] 光受容分子による外部シグナル認識・処理機構、[2] MAPキナーゼによる細胞内シグナル伝達機構、[3] アクチン細胞骨格のエネルギー変換による行動制御機構、に着目する。近年、長らく謎であった分子レベルでの情報・エネルギー変換機構が解明されつつある。講義では分子の発見から分子機構の解明に至るさまざまな研究を紹介する。そこでは、生理学的方法による現象の解析、分子遺伝学的解析による原因分子の同定、生化学的解析による変換機構の解明、生物物理学的手法による計測、構造生物学的手法を用いた可視化などを紹介し、生命現象の理解に向けた様々な方法を理解することを目指す。これらについて、小テストで理解度を深めるとともに期末試験を行う。講義途中・講義後の発言・質問も歓迎する。全１２回のうち、5回を担当

pythonを用いたプログラミング実習

本講義では、「生物における情報・エネルギー変換機構」を分子生理学的に理解することを目的とする。生物は、外的環境を素早く感知し、適切に情報処理することで環境中を生き抜いている。ここでは、[1] 光受容分子による外部シグナル認識・処理機構、[2] MAPキナーゼによる細胞内シグナル伝達機構、[3] アクチン細胞骨格のエネルギー変換による行動制御機構、に着目する。近年、長らく謎であった分子レベルでの情報・エネルギー変換機構が解明されつつある。講義では分子の発見から分子機構の解明に至るさまざまな研究を紹介する。そこでは、生理学的方法による現象の解析、分子遺伝学的解析による原因分子の同定、生化学的解析による変換機構の解明、生物物理学的手法による計測、構造生物学的手法を用いた可視化などを紹介し、生命現象の理解に向けた様々な方法を理解することを目指す。これらについて、小テストで理解度を深めるとともに期末試験を行う。講義途中・講義後の発言・質問も歓迎する。全１２回のうち、5回を担当

本講義では、「生物における情報・エネルギー変換機構」を分子生理学的に理解することを目的とする。生物は、外的環境を素早く感知し、適切に情報処理することで環境中を生き抜いている。ここでは、[1] 光受容分子による外部シグナル認識・処理機構、[2] MAPキナーゼによる細胞内シグナル伝達機構、[3] アクチン細胞骨格のエネルギー変換による行動制御機構、に着目する。近年、長らく謎であった分子レベルでの情報・エネルギー変換機構が解明されつつある。講義では分子の発見から分子機構の解明に至るさまざまな研究を紹介する。そこでは、生理学的方法による現象の解析、分子遺伝学的解析による原因分子の同定、生化学的解析による変換機構の解明、生物物理学的手法による計測、構造生物学的手法を用いた可視化などを紹介し、生命現象の理解に向けた様々な方法を理解することを目指す。これらについて、小テストで理解度を深めるとともに期末試験を行う。講義途中・講義後の発言・質問も歓迎する。全１２回のうち、４回を担当

セミナーのテーマ：ディベートを通じた学問の基礎技術の向上
ディベートとは、ある主題に対して異なる立場に分かれ、それぞれの主張を戦わせるもので、古代ギリシャから教育目的に行われている。ディベートを通じて、資料の収集、解釈、プレゼンテーション、論理的な議論のしかた、有効な反論のしかたなど多くのことを学ぶことができる。本セミナーでは、参加者をグループに分け、遺伝子組み換え、農薬、原発、ネットゲームなど賛否が分かれる主題についてディベートを行い、楽しみながらこれらの基礎技術を学んでもらうことを目標とする。

pythonを用いたプログラミング実習

本講義では、「生物における情報・エネルギー変換機構」を分子生理学的に理解することを目的とする。生物は、外的環境を素早く感知し、適切に情報処理することで環境中を生き抜いている。ここでは、[1] 光受容分子による外部シグナル認識・処理機構、[2] MAPキナーゼによる細胞内シグナル伝達機構、[3] アクチン細胞骨格のエネルギー変換による行動制御機構、に着目する。近年、長らく謎であった分子レベルでの情報・エネルギー変換機構が解明されつつある。講義では分子の発見から分子機構の解明に至るさまざまな研究を紹介する。そこでは、生理学的方法による現象の解析、分子遺伝学的解析による原因分子の同定、生化学的解析による変換機構の解明、生物物理学的手法による計測、構造生物学的手法を用いた可視化などを紹介し、生命現象の理解に向けた様々な方法を理解することを目指す。これらについて、小テストで理解度を深めるとともに期末試験を行う。講義途中・講義後の発言・質問も歓迎する。全１２回のうち、４回を担当

pythonを用いたプログラミング実習

pythonを用いたプログラミング実習

pythonを用いたプログラミング実習

pythonを使ったプログラミング実習

pythonを使ったプログラミング実習