Integrin β4 is a major target antigen in pure ocular mucous membrane pemphigoid. Reviewed

Li X, Qian H, Sogame R, Hirako Y, Tsuruta D, Ishii N, Koga H, Tsuchisaka A, Jin Z, Tsubota K, Fukumoto A, Sotozono C, Kinoshita S, Hashimoto T.

Eur J Dermatol.   Vol. 26 ( 3 ) page： 247-253   2016.1

Linear IgA/IgG bullous dermatosis reacts with multiple laminins and integrins. Reviewed

Li X, Tsuchisaka A, Qian H, Teye K, Ishii N, Sogame R, Harada K, Nakagomi D, Shimada S, Tateishi C, Hirako Y, Hashimoto T.

Eur J Dermatol   Vol. 25 ( 5 ) page： 418-423   2015.10

A Case of Subepidermal Blistering Disease with Autoantibodies to Multiple Laminin Subunits who Developed Later Autoantibodies to Alpha-5 Chain of Type IV Collagen Associated with Membranous Glomerulonephropathy. Reviewed

Sueki H, Sato Y, Ohtoshi S, Nakada T, Yoshimura A, Tateishi C, Borza DB, Fader W, Ghohestani RF, Hirako Y, Koga H, Ishii N, Tsuchisaka A, Qian H, Li X, Hashimoto T

Acta Derm Venereol   Vol. 95 ( 7 ) page： 826-829   2015.9

Pure ocular mucous membrane pemphigoid reactive with both integrin beta-4 and BP180 C-terminal domain. Reviewed

Solano-López G, Concha-Garzón MJ, Sánchez-Pérez J, Hirako Y, Li X, Ishii N, Hashimoto T, Daudén E.

Br J Dermatol.     2015.2

N-linked glycosylation on laminin γ1 influences recognition of anti-laminin γ1 pemphigoid autoantibodies. Reviewed

Li X, Qian H, Takizawa M, Koga H, Tsuchisaka A, Ishii N, Hayakawa T, Ohara K, Sitaru C, Zillikens D, Sekiguchi K, Hirako Y, Hashimoto T.

J Dermatol Sci   Vol. 77 ( 2 ) page： 125-129   2015.2

A case of oral mucous membrane pemphigoid with IgG antibodies to integrin α6β4. Reviewed

Miyamoto S, Chikazu D, Yasuda T, Enomoto A, Oh-I T, Hirako Y, Tsuchisaka A, Yasukochi A, Sogame R, Teye K, Koga H, Ishii N, Qian H, Li X, Hashimoto T.

Br J Dermatol.     2014.12

A case of concurrent antilaminin γ1 pemphigoid and antilaminin-332-type mucous membrane pemphigoid. Reviewed

Li X, Qian H, Ishii N, Yamaya M, Fukuda H, Mukai H, Hirako Y, Hashimoto T.

Br J Dermatol     2014.11

Major cleavage-dependent epitopes for linear IgA bullous dermatosis are formed at the boundary between the non-collagenous 16A and collagenous 15 domains of BP180. Reviewed

Yamauchi T, Matsushita S, Hashimoto T, Hirako Y.

J Dermatol Sci.   Vol. 76 ( 1 ) page： 25-33   2014.10

Isolation of a hemidesmosome-rich fraction from a human squamous cell carcinoma cell line. Reviewed

Hirako Y, Yonemoto Y, Yamauchi T, Nishizawa Y, Kawamoto Y, Owaribe K.

Exp Cell Res.   Vol. 324 ( 2 ) page： 172-182   2014.7

眼型粘膜類天疱瘡

十亀良介、石井文人、古賀浩嗣、平子善章、橋本隆

Visual Dermatology   Vol. 12 ( 2 ) page： 168-169   2013.1

Plectin deficiency leads to both muscular dystrophy and pyloric atresia in epidermolysis bullosa simplex. Reviewed

Natsuga K, Nishie W, Shinkuma S, Arita K, Nakamura H, Ohyama M, Osaka H, Kambara T, Hirako Y, Shimizu H.

Hum Mutat   Vol. 31 ( 10 ) page： 1687-1698   2010.10

Plectin expression patterns determine two distinct subtypes of epidermolysis bullosa simplex. Reviewed

Natsuga K, Nishie W, Akiyama M, Nakamura H, Shinkuma S, McMillan JR, Nagasaki A, Has C, Ouchi T, Ishiko A, Hirako Y, Owaribe K, Sawamura D, Bruckner-Tuderman L, Shimizu H.

Hum Mutat   Vol. 31 ( 3 ) page： 308-316   2010.3

IgG from patients with bullous pemphigoid depletes cultured keratinocytes of the 180-kDa bullous pemphigoid antigen (type XVII collagen) and weakens cell attachment. Reviewed

Iwata H, Kamio N, Aoyama Y, Yamamoto Y, Hirako Y, Owaribe K, Kitajima Y.

J Invest Dermatol   Vol. 129 ( 4 ) page： 919-926   2009.4

Cell adhesion and the cytoskeleton Invited

  Vol. 80 ( 3 ) page： 184-193   2008.3

Hepatic stellate cells express synemin, a protein bridging intermediate filaments to focal adhesions. Reviewed

Uyama N, Zhao L, Van Rossen E, Hirako Y, Reynaert H, Adams DH, Xue Z, Li Z, Robson R, Pekny M, Geerts A.

Gut   Vol. 55   page： 1276-1289   2006

*Both type-I hemidesmosomes and adherens-type junctions contribute to the cell-substratum adhesion system in myoepithelial cells. Reviewed

Uematsu J, Nishizawa Y, Hirako Y, Kitamura K, Usukura J, Miyata T, Owaribe K.

Eur J Cell Biol   Vol. 84   page： 407-415   2005

IgG autoantibodies to type VII collagen and an exclusive IgG3 reactivity to the laminin alpha3 chain in a patient with an autoimmune subepidermal blistering disease. Reviewed

Baican A, Hirako Y, Lazarova Z, Yancey KB, Zillikens D, Sitaru C.

J Am Acad Dermatol   Vol. 53 ( 2 ) page： 517-522   2005

Anti-p200 pemphigoid--a new bullous autoimmune dermatosis Invited Reviewed

Shimanovich I, Rose C, Hirako Y, Butt-Dorje E, Zillikens D.

J Dtsch Dermatol Ges   Vol. 2 ( 1 ) page： 7-14   2004

*The 97-kDa (LABD97) and 120-kDa (LAD-1) fragments of bullous pemphigoid antigen 180/type XVII collagen have different N-termini. Reviewed

Hirako Y, Nishizawa Y, Sitaru C, Opitz A, Marcus K, Meyer HE, Butt E, Owaribe K, Zillikens D.

J Invest Dermatol   Vol. 121 ( 6 ) page： 1554-1556   2003

Extracellular cleavage of bullous pemphigoid antigen 180/type XVII collagen and its involvement in hemidesmosomal disassembly. Reviewed

Hirako Y, Yoshino K, Zillikens D, Owaribe K.

J Biochem   Vol. 133 ( 2 ) page： 197-206   2003

Characterization of mammalian synemin, an intermediate filament protein present in all four classes of muscle cells and some neuroglial cells: co-localization and interaction with type III intermediate filament proteins and keratins. Reviewed

Hirako Y, Yamakawa H, Tsujimura Y, Nishizawa Y, Okumura M, Usukura J, Matsumoto H, Jackson KW, Owaribe K, Ohara O.

Cell Tissue Res   Vol. 313 ( 2 ) page： 195-207   2003

Molecular architecture of hemidesmosomes and desmosomes. Invited

Hirako Y, Owaribe K.

Seikagaku   Vol. 71 ( 12 ) page： 1417-1431   1999

Hemidesmosomes and their unique transmembrane protein BP180. Invited Reviewed

Hirako Y, Owaribe K.

Microsc Res Tech   Vol. 43 ( 3 ) page： 207-217   1998

*Cleavage of BP180, a 180-kDa bullous pemphigoid antigen, yields a 120-kDa collagenous extracellular polypeptide. Reviewed

Hirako Y, Usukura J, Uematsu J, Hashimoto T, Kitajima Y, Owaribe K.

J Biol Chem   Vol. 273 ( 16 ) page： 9711-9717   1998

*Demonstration of the molecular shape of BP180, a 180-kDa bullous pemphigoid antigen and its potential for trimer formation. Reviewed

Hirako Y, Usukura J, Nishizawa Y, Owaribe K.

J Biol Chem   Vol. 271 ( 23 ) page： 13739-137345   1996

Hemidesmosomes contribute to the mechanical strength of epithelial cell sheets adhering to the substrate

2022.6.28 

Hemidesmosomes contribute to the mechanical strength of epithelial cell sheets adhering to the substrate

Kou Hashimoto, Yoshiaki Hirako

2021.6.26 

XVII型コラーゲン欠失培養ラット表皮角化細胞株の作製とヘミデスモソーム形成への影響の解析

藤岡光、平子善章

第52回日本結合組織学会学術大会  2020.9.19 

基底膜タンパク質ラミニン332の沈着パターン形成におけるプレクチンの機能の検証

浅倉亮佑、平子善章

第52回日本結合組織学会学術大会  2020.9.19 

Generation of cultured human cells deficient in dermal-epidermal adhesion apparatus by genome editing International conference

Yoshiaki Hirako, Ryotaro Kamo and Tatsuya Hiraki.

Japan - Singapore International Skin Conference 2019 

Distinct roles of two plakin proteins in type I hemidesmosomes

ヘミデスモソーム構造における2種類のプラーキンタンパク質の役割の解析

近藤擁, 加茂遼太郎, 平子善章

第49回日本結合組織学会学術大会 

Knockout of type XVII collagen in DJM-1 cells affects structure of type I hemidesmosomes and deposition pattern of laminin-332 matrix

The 27th CDB meeting "Body Surface Tactics" 

XVII型コラーゲンを欠失したDJM-1細胞は、I型ヘミデスモソーム形成とラミニン332マトリックス沈着パターンに異常を示す

山内友恵、加茂遼太郎、平子善章

第68回日本細胞生物学会大会 

DJM-1 cells deficient in BP180/type XVII collagen exhibit rudiment hemidesmosomes and abnormal pattern of laminin-332 matrix

Yoshiaki Hirako, Ryotaro Kamo , Minori Kaneda

DJM-1細胞を用いたヘミデスモソームの基礎研究 Invited

平子善章

第３７回水疱症研究会 

Type XVII collagen plays an important role in the formation of type I hemidesmosomes and the laminin-332 matrix assembly

Major cleavage-dependent epitopes for linear IgA bullous dermatosis are formed at the boundary between the NC16A and C15 domains of BP180

A low calcium culture condition promotes deposition of laminin-332 and formation of hemidesmosomes in a squamous carcinoma cell line

Yoshiaki Hirako, Yuki Yonemoto, Tomoe Yamauchi, Yuji Nishizawa, Yoshiyuki Kawamoto, Katsushi Owaribe

Ectodomain shedding of type XVII collagen induces formation of cleavage-dependent autoantigenic epitopes

Tomoe Yamauchi, Satoshi Matsushita, Takashi Hashimoto, Yoshiaki Hirako

基礎研究者の視点から見たヘミデスモソームと自己免疫性皮膚疾患 Invited

平子善章

第365回日本皮膚科学会福岡地方会 

A muscle specific isoform of LMO7 localizes to costamere, a cell-substrate adhesion site in striated muscle

An epithelial isoform of LMO7 interacts with actin cytoskeleton through a novel functional region at its N-terminus International conference

Preparation of the hemidesmosome-rich fraction from a human squamous cell carcinoma line DJM-1

Yoshiaki Hirako, Yuki Yonemoto, Katsushi Owaribe

Large scale study defined human β4 integrin as the major autoantigen for pure ocular mucous membrane pemphigoid

Ryosuke Sogame, Yoshiaki Hirako, Daisuke Tsuruta, Norito Ishii, Teruki Dainichi, Hiroshi Koga, Shunpei Fukuda, Bungo Ohyama, Takahiro Hamada, Tadashi Karashima, Takekuni Nakama, Katsushi Owaribe, Kazuo Tsubota, Shigeru Kinoshita, Takashi Hashimoto

A monoclonal antibody and Linear IgA bullous dermatosis autoantibodies preferentially recognize epitopes within the NC16A domain of BP180 International conference

Satoshi Matsushita, Takashi Hashimoto, Katsushi Owaribe, Yoshiaki Hirako

単層上皮におけるヘミデスモソーム関連タンパク質の分布パターン

富樫宏一、植松淳、尾張部克志、平子善章

第42回日本結合組織学会学術大会・第57回マトリックス研究会大会合同学術集会 

Cell to Extracellular Matrix Adhesion Structures in Stratified and Simple Epithelia Invited

Yoshiaki Hirako

Localization of a cleavage-dependent epitope within the non-collagenous 16A domain of the 180 kDa bullous pemphigoid antigen

Satoshi Matsushita, Katsushi Owaribe, Yoshiaki Hirako

Analyses of hemidesmosome-rich fraction prepared from a human squamous cell carcinoma line

Yuki Yonemoto, Tomoya Katsura, Katsushi Owaribe, Yoshiaki Hirako

Calcium influences Hemidesmosome Formation and Processing of Laminin332

Yoshiaki Hirako, Yuki Yonemoto, Tomoya Katsura, Katsushi Owaribe

8th Pan-Pacific Connective Tissue Society Symposium 

Preparation of the hemidesmosome-rich fraction from a human squamous cell carcinoma cell line

Gastrointestinal distribution of the 180-kDa bullous pemphigoid antigen International conference

Post IID 2008 satellite internaional meeting on autoimmne bullous diseases 

Plasminogen system mediates the C-terminal cleavage of the 120-kDa LAD-1 producing a 97-kDa polypeptide

The 31st annual meeting of the Japanese society for investigative dermatology, Kyoto, 2006, 

Characerization of type XII collagen as an adhesive ligand for keratinocytes

The 31st annual meeting of the Japanese society for investigative dermatology, Kyoto 

Identification of 350-kDa polypeptide of the isolated hemidesmosome fraction as type XII collagen

The 58th annual meeting of Japan society for cell biology 

Serine protease inhibitors suppress the C-terminal cleavage of the soluble ectodomain of type XVII collagen. International conference

Meeting of special priority program1086 -Genetic and molecular analysis of basement membranes and its anchorage-. Kloster Irsee, Germany 

Plasminogen system mediates the C-terminus cleavage of the 120-kDa soluble ectodomain of the type XVII collagen/BP180 resulting in the 97-kDa fragment.

The 57th annual meeting of Japan society for cell biology, Osaka 

Single-molecule tracking/dragging revealed transient actin severing in the membrane skeleton as a mechanism for intercompartmental hot movement of membrane molecules

Annual meeting of the biophysical society (U. S.), Baltimore, MD, USA 

１８０ｋＤ類天疱瘡抗原の細胞外Ｃ末端部位の切断機構の解明および疾患との関連の検証

Grant number：17790762  2005.4 - 2007.3

科学研究費補助金  若手研究(B)

平子　善章

基礎生物学演習I

2018

基礎細胞生物学

2018

生物学実験

2018

基礎生物学演習I

2017

基礎細胞生物学

2017

生物学実験

2017

基礎生物学演習I

2016

基礎細胞生物学

2016

生物学実験

2016

基礎生物学演習I

2015

基礎細胞生物学

2015

生物学実験

2015

基礎生物学演習I

2014

基礎細胞生物学

2014

生物学実験

2014

基礎生物学演習I

2013

生物学実験

2013

生物学基礎I

2013

基礎細胞生物学

2013

生物学実験

2013

基礎セミナー

2013

基礎細胞学

2012

生物学基礎I

2012

生物学実験

2012

基礎生物学演習I

2011

基礎細胞生物学

2011

基礎細胞生物学

2010