Updated on 2024/03/23

写真a

 
MINO, Hiroyuki
 
Organization
Graduate School of Science Associate professor
Graduate School
Graduate School of Science
Undergraduate School
School of Science
Title
Associate professor
Contact information
メールアドレス
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Degree 1

  1. 博士(理学) ( 1995.11   関西学院大学 ) 

Research Interests 9

  1. 生体エネルギー

  2. 磁気緩和

  3. 磁気共鳴

  4. 生物物理

  5. パルスESR

  6. ESR

  7. 光合成

  8. パルスEPR

  9. EPR

Research Areas 5

  1. Others / Others  / Chemistry Related to Living Body

  2. Others / Others  / Physical Chemistry

  3. Others / Others  / Physical Properties II

  4. Others / Others  / Living Organism Physics/Chemical Physics

  5. Others / Others  / Living Organism Molecular Science

Current Research Project and SDGs 2

  1. 電子スピン共鳴法(ESR)による酸素発生系の研究

  2. パルスESR法によるタンパク質の構造解析

Research History 6

  1. 名古屋大学大学院理学研究科(物質理学専攻)准教授

    2008.4

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    Country:Japan

  2. 名古屋大学大学院理学研究科(物質理学専攻)助教授

    2006.7 - 2007.3

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    Country:Japan

  3. 名古屋大学大学院理学研究科(物質理学専攻)助手

    2001.1 - 2006.7

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    Country:Japan

  4. 理化学研究所 フロンティア研究員(光生物研究チーム)

    1999.10 - 2001.1

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    Country:Japan

  5. 理化学研究所 基礎科学特別研究員(光合成科学研究室、光生物研究チーム)

    1996.10 - 1999.9

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    Country:Japan

  6. 理化学研究所 協力研究員(光合成化学研究室)

    1996.4 - 1996.9

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    Country:Japan

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Education 2

  1. Kwansei Gakuin University   Graduate School, Division of Natural Science   physics

    - 1995

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    Country: Japan

  2. Kwansei Gakuin University   Faculty of Science   Physics

    1986.4 - 1990.3

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    Country: Japan

Professional Memberships 4

  1. 電子スピンサイエンス学会

  2. 日本生物物理学会

  3. 日本物理学会

  4. 日本植物生理学会

Committee Memberships 5

  1. 日本生物物理学会   専門委員  

    2021.1   

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    Committee type:Academic society

  2. 電子スピンサイエンス学会   理事  

    2020.1   

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    Committee type:Academic society

  3. 日本生物物理学会   専門委員  

    2019.1 - 2019.12   

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    Committee type:Academic society

  4. 電子スピンサイエンス学会   理事  

    2018.1 - 2019.12   

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    Committee type:Academic society

  5. 電子スピンサイエンス学会   理事  

    2014.1 - 2015.12   

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    Committee type:Academic society

Awards 1

  1. 電子スピンサイエンス学会奨励賞

    2007.11   電子スピンサイエンス学会  

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    Country:Japan

 

Papers 81

  1. Insights into the protonation state and spin structure for the g = 2 multiline electron paramagnetic resonance signal of the oxygen-evolving complex.

    Saito K, Nishio S, Asada M, Mino H, Ishikita H

    PNAS nexus   Vol. 2 ( 8 ) page: pgad244   2023.8

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1093/pnasnexus/pgad244

    PubMed

  2. Molecular Structure Related to an S =5/2 High-Spin S-2 State Manganese Cluster of Photosystem II Investigated by Q-Band Pulse EPR Spectroscopy

    Kosaki Shinya, Mino Hiroyuki

    JOURNAL OF PHYSICAL CHEMISTRY B   Vol. 127 ( 29 ) page: 6441 - 6448   2023.7

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1021/acs.jpcb.3c01656

    Web of Science

    PubMed

  3. A [4Fe-4S] cluster resides at the active center of phosphomevalonate dehydratase, a key enzyme in the archaeal modified mevalonate pathway

    Komeyama Mutsumi, Kanno Kohsuke, Mino Hiroyuki, Yasuno Yoko, Shinada Tetsuro, Ito Tomokazu, Hemmi Hisashi

    FRONTIERS IN MICROBIOLOGY   Vol. 14   page: 1150353   2023.3

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.3389/fmicb.2023.1150353

    Web of Science

    PubMed

  4. Protonation structure of the closed-cubane conformation of the O(2)-evolving complex in photosystem II.

    Saito K, Mino H, Nishio S, Ishikita H

    PNAS nexus   Vol. 1 ( 5 ) page: pgac221   2022.11

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1093/pnasnexus/pgac221

    PubMed

  5. Location of two Mn2+ affinity sites in photosystem II detected by pulsed electron-electron double resonance

    Mino Hiroyuki, Asada Mizue

    PHOTOSYNTHESIS RESEARCH   Vol. 152 ( 3 ) page: 289 - 295   2022.6

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1007/s11120-021-00885-5

    Web of Science

    PubMed

  6. Extremely low-frequency pulses of faint magnetic field induce mitophagy to rejuvenate mitochondria

    Toda Takuro, Ito Mikako, Takeda Jun-ichi, Masuda Akio, Mino Hiroyuki, Hattori Nobutaka, Mohri Kaneo, Ohno Kinji

    COMMUNICATIONS BIOLOGY   Vol. 5 ( 1 ) page: 453   2022.5

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    Language:Japanese   Publishing type:Research paper (scientific journal)  

    DOI: 10.1038/s42003-022-03389-7

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    PubMed

  7. Temperature dependence of the formation of the g similar to 5 EPR signal in the oxygen evolving complex of photosystem II

    Mino Hiroyuki

    PHOTOSYNTHESIS RESEARCH   Vol. 152 ( 2 ) page: 207 - 212   2022.5

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    Language:Japanese   Publishing type:Research paper (scientific journal)  

    DOI: 10.1007/s11120-022-00916-9

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    PubMed

  8. The evolutionary conserved iron-sulfur protein TCR controls P700 oxidation in photosystem I

    Trinh Mai Duy Luu, Miyazaki Daichi, Ono Sumire, Nomata Jiro, Kono Masaru, Mino Hiroyuki, Niwa Tatsuya, Okegawa Yuki, Motohashi Ken, Taguchi Hideki, Hisabori Toru, Masuda Shinji

    ISCIENCE   Vol. 24 ( 2 ) page: 102059   2021.2

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    Language:Japanese   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.isci.2021.102059

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    PubMed

  9. Location of two Mn2+ affinity sites in photosystem II detected by pulsed electron–electron double resonance Invited Reviewed

    Mino, H and Asada, M

    photosynthesis research     2021

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    Authorship:Lead author, Corresponding author   Language:English  

  10. Formation of the High-Spin S2 State Related to the Extrinsic Proteins in the Oxygen Evolving Complex of Photosystem II. International journal

    Shota Taguchi, Liangliang Shen, Guangye Han, Yasufumi Umena, Jian-Ren Shen, Takumi Noguchi, Hiroyuki Mino

    The journal of physical chemistry letters   Vol. 11 ( 20 ) page: 8908 - 8913   2020.10

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    Language:English   Publishing type:Research paper (scientific journal)  

    The high-spin S2 state was investigated with photosystem II (PSII) from spinach, Thermosynechococcus vulcanus, and Cyanidioschyzon merolae. In extrinsic protein-depleted PSII, high-spin electron paramagnetic resonance (EPR) signals were not detected in either species, whereas all species showed g ∼ 5 signals in the presence of a high concentration of Ca2+ instead of the multiline signal. In the intact and PsbP/Q-depleted PSII from spinach, the g = 4.1 EPR signal was detected. These results show that formation of the high-spin S2 state of the manganese cluster is regulated by the extrinsic proteins through a charge located near the Mn4 atom in the Mn4CaO5 cluster but is independent of the intrinsic proteins. The shift to the g ∼ 5 state is caused by tilting of the z-axis in the Mn4 coordinates through hydrogen bonds or external divalent cations. The structural modification may allow insertion of an oxygen atom during the S2-to-S3 transition.

    DOI: 10.1021/acs.jpclett.0c02411

    Web of Science

    PubMed

  11. Molecular Structure of the S-2 State with a g=5 Signal in the Oxygen Evolving Complex of Photosystem II

    Taguchi Shota, Noguchi Takumi, Mino Hiroyuki

    JOURNAL OF PHYSICAL CHEMISTRY B   Vol. 124 ( 27 ) page: 5531 - 5537   2020.7

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    Language:Japanese   Publishing type:Research paper (scientific journal)  

    DOI: 10.1021/acs.jpcb.0c02913

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    PubMed

  12. Orientation of Ligand Field for Dangling Manganese in Photosynthetic Oxygen-Evolving Complex of Photosystem II Reviewed

    Hiroyuki Mino, Hiroki Nagashima

    The Journal of Physical Chemistry B   Vol. 124 ( 1 ) page: 128 - 133   2020.1

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:American Chemical Society ({ACS})  

    DOI: 10.1021/acs.jpcb.9b10817

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    PubMed

  13. Flavin-Radical Formation in the Light-Oxygen-Voltage-Sensing Domain of the Photozipper Blue-light Sensor Protein. Reviewed International journal

    Tsukuno H, Ozeki K, Kobayashi I, Hisatomi O, Mino H

    The journal of physical chemistry. B   Vol. 122 ( 38 ) page: 8819 - 8823   2018.9

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    Language:Japanese   Publishing type:Research paper (scientific journal)  

    Formation of the neutral flavin radical in the light-oxygen-voltage-sensing (LOV-sensing) domain of photozipper, based on VfAUREO1, was investigated by electron paramagnetic resonance spectroscopy. The flavin radical was observed in the presence of dithiothreitol by illumination of a LOV-domain mutant (C254S), in which a photoactive cysteine residue in close proximity to flavin was replaced with a serine. The radical did not form under low initial protein-concentration conditions (less than 20 μM). The flavin radicals accumulated with logistic time-dependent kinetics when the protein concentrations were higher than 30 μM. These results indicate that the radical is produced by concerted reactions involving protein interactions and that the radical is formed from the LOV dimer but not the LOV monomer. In contrast, logistic time dependencies were not observed for the sample adapted to the dark following radical formation by illumination, indicating that initialization of the proton pathway is essential for this fast sensing reaction.

    DOI: 10.1021/acs.jpcb.8b05808

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    PubMed

  14. Local Structural Modification of Ca2+-Depleted Photosystem II Detected by Proton Matrix ENDOR Reviewed

    Hiroki Nagashima, Hiroyuki Mino

    Applied Magnetic Resonance   Vol. 49 ( 8 ) page: 803 - 812   2018.8

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Springer-Verlag Wien  

    Angular dependence of the proton matrix electron nuclear double resonance (ENDOR) spectra was investigated in the oriented Ca2+-depleted Photosystem II (PS II). Six pairs of the proton signals have been previously detected in the untreated PS II and one of the six pairs has been disappeared in the Ca2+-depleted PS II (Nagashima and Mino, J Biol Chem 290:28166–28174, 2015), where the proton signals with 4 MHz separation were lost and assigned to the proton closest to the terminal Mn, labeled as Mn4 in the Mn cluster. In the oriented Ca2+-depleted PS II, the proton signal with 4 MHz separation was disappeared when the external magnetic field is parallel to the membrane normal n (θ = 0°). A pair of broad ENDOR signals with 1.4–2.0 MHz hyperfine coupling was detected in the oriented Ca2+-depleted PS II at θ = 90°. These results indicate the Ca2+-depletion derives the rearrangement of the hydrogen bonding of the water molecule surrounded Mn cluster.

    DOI: 10.1007/s00723-018-1006-1

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    Scopus

  15. Location of the extrinsic subunit PsbP in photosystem II studied by pulsed electron-electron double resonance

    Asada Mizue, Nishimura Taishi, Ifuku Kentaro, Mino Hiroyuki

    BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS   Vol. 1859 ( 5 ) page: 394-399   2018.5

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.bbabio.2018.03.002

    Web of Science

  16. Dimeric Structure of the Blue Light Sensor Protein Photozipper in the Active State

    Ozeki Kohei, Tsukuno Hiroyuki, Nagashima Hiroki, Hisatomi Osamu, Mino Hiroyuki

    BIOCHEMISTRY   Vol. 57 ( 5 ) page: 494-497   2018.2

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    DOI: 10.1021/acs.biochem.7b01045

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  17. Magnetic Structure of Manganese Cluster in Photosystem II Investigated by Electron Paramagnetic Resonance Invited Reviewed

    Nagashima, H.,Asada, M. , Mino, H.

    Biophys. Physicobiol.   Vol. 15   page: 45-50   2018

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  18. Magnetic structure of manganese cluster in photosystem II investigated by electron paramagnetic resonance

    Nagashima Hiroki, Asada Mizue, Mino Hiroyuki

    Biophysics and Physicobiology   Vol. 15 ( 0 ) page: 45 - 50   2018

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:The Biophysical Society of Japan  

    <p>The electronic structure of manganese (Mn) cluster in photosystem II was investigated by electron paramagnetic resonance (EPR) spectroscopy. In order to determine the spin density distribution in magnetically coupled Mn in the S<sub>2</sub> state Mn cluster, pulsed electron–electron double resonance (PELDOR) measurement was performed. The local environment of the Mn cluster was investigated by electron-nuclear double resonance (ENDOR). Using spin projections determined by PELDOR, ENDOR signals were assigned to the water molecules ligated to the Mn cluster. The location of a high-affinity Mn<sup>2+</sup> site in apo-photosystem II, which is the initial site of photoactivation of the Mn cluster, was determined by PELDOR.</p>

    DOI: 10.2142/biophysico.15.0_45

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    PubMed

    CiNii Research

  19. Mechanism of Photodamage of the Oxygen Evolving Mn Cluster of Photosystem II by Excessive Light Energy

    Zavafer Alonso, Koinuma Wataru, Chow Wah Soon, Cheah Mun Hon, Mino Hiroyuki

    SCIENTIFIC REPORTS   Vol. 7   2017.8

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    DOI: 10.1038/s41598-017-07671-1

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  20. Hyperfine Sublevel Correlation Spectroscopy Studies of Iron-Sulfur Cluster in Rieske Protein from Green Sulfur Bacterium Chlorobaculum tepidum

    Nagashima Hiroki, Kishimoto Hiraku, Mutoh Risa, Terashima Naotaka, Oh-oka Hirozo, Kurisu Genji, Mino Hiroyuki

    JOURNAL OF PHYSICAL CHEMISTRY B   Vol. 121 ( 12 ) page: 2543-2553   2017.3

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    DOI: 10.1021/acs.jpcb.6b12968

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  21. Regulated Electron Tunneling of Photoinduced Primary Charge Separated State in the Photosystem II Reaction Center

    Hasegawa Masashi, Nagashima Hiroki, Minobe Reina, Tachikawa Takashi, Mino Hiroyuki, Kobori Yasuhiro

    JOURNAL OF PHYSICAL CHEMISTRY LETTERS   Vol. 8 ( 6 ) page: 1179-1184   2017.3

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1021/acs.jpclett.7b00044

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  22. Regulated Electron Tunneling of Photoinduced Primary Charge- Separated State in the Photosystem II Reaction Center Reviewed

    Hasegawa,M., Nagashima, H., Minobe, R., Tachikawa,T., Mino,H. and Kobori,Y.

    J. Phys.Chem. Lett.     page: in press   2017.3

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  23. Location of Methanol on the S-2 State Mn Cluster in Photosystem II Studied by Proton Matrix Electron Nuclear Double Resonance

    Nagashima Hiroki, Mino Hiroyuki

    JOURNAL OF PHYSICAL CHEMISTRY LETTERS   Vol. 8 ( 3 ) page: 621-625   2017.2

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    DOI: 10.1021/acs.jpclett.7b00110

    Web of Science

  24. Mechanism of Photodamage of the Oxygen Evolving Mn Cluster of Photosystem II by Excessive Light Energy Reviewed

    Zavafer, A., Koinuma, W., Chow, S. W., Cheah, H. M. and Mino, H.

      Vol. 7   page: 7604   2017

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  25. Hyperfine Sublevel Correlation Spectroscopy Studies of Iron-Sulfur Cluster in Rieske Protein from Green Sulfur Bacterium Chlorobaculum tepidum Reviewed

    Nagashima,H., Kishimoto, H., Mutoh, R., Terashima, N., Oh-oka,H., Kurisu, G.and Mino, H.,

    J. Phys.Chem. B   Vol. 121   page: 2543-2553   2017

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  26. ESR分光法による光化学系IIの酸素発生系Mnクラスターの構造解析 Invited Reviewed

    浅田瑞枝、長嶋宏樹、三野広幸

    生物物理   Vol. 57   page: 1-4   2017

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  27. Electronic Structure of Manganese Cluster in Photosystem II Investigated by ESR

    ASADA Mizue, NAGASHIMA Hiroki, MINO Hiroyuki

    Seibutsu Butsuri   Vol. 57 ( 1 ) page: 011 - 014   2017

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    Language:Japanese   Publishing type:Research paper (scientific journal)   Publisher:The Biophysical Society of Japan General Incorporated Association  

    <p>Electronic structure of Manganese cluster in photosystem II was investigated by Electron Spin Resonance (ESR) spectroscopy. Spin projections on Mn ions in S<sub>2</sub> state manganese cluster were determined by Pulsed Electron-Electron Double Resonance (PELDOR). Protons surrounded manganese cluster were detected by Electron Nuclear Double Resonance (ENDOR). Based on obtained spin projections, ENDOR signals were assigned to the water molecules ligated to manganese cluster. PELDOR technique was applied to the determination of the high affinity Mn<sup>2+</sup> site in apo-photosystem II, which is the key site of photo-activation process of manganese cluster.</p>

    DOI: 10.2142/biophys.57.011

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    CiNii Research

  28. Location of the High-Affinity Mn2+ Site in Photosystem II Detected by PELDOR Reviewed

    Asada, M and Mino, H.

    Jounanl of physical chemistry   Vol. 119 ( 32 ) page: 10139-10144   2015

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  29. Generalized approximate spin projection calculations of effective exchange integrals of the CaMn4O5 cluster in the S1 and S3 states of the oxygen evolving complex of photosystem I Reviewed

    Isobe,H., Shoji,M., Yamanaka,S., Mino, H., Umena,Y., Kawakami,K., Kamiya,N., Shen,J.-R. and Yamaguchi, K

    Phys. Chem. Chem. Phys   Vol. 16   page: 11911-11923   2014

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  30. Site-directed spin labeling-electron spin resonance mapping of the residues of cyanobacterial clock protein KaiA that are affected by KaiA-KaiC interaction Reviewed

    Isobe,H., Shoji,M., Yamanaka,S., Mino, H., Umena,Y., Kawakami,K., Kamiya,N., Shen,J.-R. and Yamaguchi, K

    Genes to Cells   Vol. 19   page: 297-324   2014

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  31. Electronic structure of S2 state of the oxygen-evolving complex of photosystem II studied by PELDOR Reviewed

    Mizue Asada, Hiroki Nagashima, Faisal Hammad Mekky Koua, Jian-Ren Shen, Asako Kawamori and Hiroyuki Mino

    Biochimica et Biophysica Acta     page: in press   2013

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    DOI: org/10.1016/j.bbabio.2012.12.011

  32. Highly resolved proton matrix ENDOR of oriented Photosystem II membranes in the S2 state Reviewed

    Nagashima, H. and Mino, H.

    Biochim. Biophys. Acta   Vol. 1827   page: 1165-1173   2013

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    Proton matrix ENDOR was performed to investigate the protons close to the manganese cluster in oriented samples of photosystem II (PS II). Eight pairs of ENDOR signals were detected in oriented PS II membranes. At an angle of theta = 0º between the membrane normal vector n and the external field H0, five pairs of ENDOR signals were exchangeable in D2O medium and three pairs were not exchangeable in D2O medium. The hyperfine splitting of 3.60 MHz attheta =0º increased to 3.80 MHz at theta=90º. The non-exchangeable signals with 1.73 MHz hyperfine splitting at theta =0º, which were assigned to a proton in an amino acid residue, were not detected at theta =90º in oriented PS II or in non-oriented PS II. Highly resolved spectra show that only limited numbers of protons were detected by CW-ENDOR spectra, although many protons were located near the CaMn4O5 cluster. The detected exchangeable protons were proposed to arise from the protons belonging to the water molecules, labeled W1-W4 in the 1.9 Å crystal structure, directly ligated to the CaMn4O5 cluster, and nearby amino-acid residue.

  33. Nonselective excitation of pulsed ELDOR using multi-frequency microwaves Reviewed

    Yuki Asada, Risa Mutoh, Masahiro Ishiura and Hiroyuki Mino

    Journal of Magnetic Resonance   Vol. 213   page: 200-205   2011.12

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  34. Temperature dependence of relaxation time of a stable radical pair in SyPixD investigated by pulsed EPR Reviewed

    Toru Kondo, Shinji Masuda, Kazuhiko Tsutsui and Hiroyuki Mino

    Chemical physics Letter   Vol. 501   page: 528-533   2011

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  35. Pulsed EPR analysis of the photo-induced triplet radical pair in the BLUF protein SyPixD: Determination of the protein-protein distance and orientation in the oligomeric protein Reviewed

    Toru Kondo, Shinji Masuda and Hiroyuki Mino

    Applied Magnetic Resonance     page: in press   2011

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  36. Thermodynamically induced conformational changes of the cyanobacterial circadian clock protein KaiB Reviewed

    Risa Mutoh, Hiroyuki Mino, Reiko Murakami, Tatsuya Uzumaki, Masahiro Ishiura

    Applied Magnetic Resonance     page: in press   2011

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  37. Interaction and Inhibitory Effect of Ammonium Cation in the Oxygen Evolving Center of Photosytem II Reviewed

    Masaya Tsuno, Hiroyuki Suzuki, Toru Kondo, Hiroyuki Mino, and Takumi Noguchi

    Biochemistry   Vol. 50   page: 2506-2514   2011

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  38. Monitoring the Aggregation Processes of Amyloid-ß Using a Spin-Labeled, Fluorescent Nitroxyl Radical Reviewed

    Fumiya Mito, Toshihide Yamasaki,Yuko Ito, Mayumi Yamato,Hiroyuki Mino, Hiromi Sadasue,Chisato Shirahama,Kiyoshi Sakai, Hideo Utsumi and Ken-ichi Yamada

    Chemical Communications   Vol. 47   page: 5070-5072   2011

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  39. Direct interaction between KaiA and KaiB revealed by a site-directed spin labeling electron spin resonance analysis Reviewed

    Risa Mutoh, Hiroyuki Mino, Reiko Murakami, Tatsuya Uzumaki, Atsushi Takabayashi1a, Kentaro Ishii and Masahiro Ishiura

    Genes to Cells   Vol. 15   page: 269–280   2010

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  40. Unique Biogenesis of High-molecular-mass Multimeric Metalloenzyme Nitrile Hydratase: Intermediates, and a Proposed Mechanism for Self-Subunit Swapping Maturation Reviewed

    Zhemin Zhou , Yoshiteru Hashimoto , Tianwei Cui , Yumi Washizawa , Hiroyuki Mino , and Michihiko Kobayashi

    Biochemistry   Vol. 49   page: 9638-9648   2010

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  41. Peroxide Decoloration of CI Acid Orange 7 Catalyzed by Manganese Chlorophyll Derivatives at the Surfaces of Micelles and Lipid Bilayers Reviewed

    Shuichi Ishigure, Tatsuro Mitsui, Shingo Ito, Yuji Kondo, Shigeki Kawabe, Masaharu Kondo, Takehisa Dewa, Hiroyuki Mino, Shigeru Itoh and Mamoru Nango

    Langmuir   Vol. 26   page: ,7774-7782   2010

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  42. Characterization of a blue-copper protein, auracyanin, of the filamentous anoxygenic phototrophic bacterium Roseiflexus castenholzii Reviewed

      Vol. 490 ( 1 ) page: 57-62   2009.10

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  43. Remarkably Stabilized Charge Separations in Inorganic Nanospace Reviewed

    Yui,T., Tsuchino,T., Mino,H., Kajino,T., Itoh,S., Fukushima,Y. and Takagi, K.

    Bull. Chem. Soc. Jpn.   Vol. 82 ( 7 ) page: 914-916   2009

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  44. Formation of Interacting Spins on Flavosemiquinone and Tyrosine Radical in Photoreaction of a Blue Light Sensor BLUF Protein TePixD Reviewed

    Nagai Hiroko, Fukushima Yoshimasa, Okajima Koji, Ikeuchi Masahiko, Mino Hiroyuki

    BIOCHEMISTRY   Vol. 47 ( 47 ) page: 12574-12582   2008.11

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1021/bi8010187

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  45. *Formation of interacting spins on flavosemiquinone and tyrosine radical in photoreaction of a blue light sensor BLUF protein TePixD Reviewed

    Nagai, H. and Fukushima, Y., Okajima, K., Ikeuchi, M and Mino, H.

    Biochemistry   Vol. 47   page: 12574-12582   2008

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

  46. The difference in microenvironments and functions of tyrosine radicals YZ and YD in photosystem II studied by EPR Invited Reviewed

    Mino, H. and Kawamori, A.

    Photosyn. Res.   Vol. 98   page: 151-157   2008

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  47. *An Electron Spin-Polarized Signal of the P800+A1(Q)- State in the Homodimeric Reaction Center Core Complex of Heliobacterium modesticaldum Reviewed

    Miyamoto, R., Mino, H., Kondo, T., Itoh, S. and Oh-oka, H.

    biochemistry   Vol. 47   page: 4386   2008

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  48. Function of chlorophyll d in reaction Centers of photosysterns I and II of the oxygenic photosynthesis of acaryochloris marina Reviewed

    Itoh Shigeru, Mino Hiroyuki, Itoh Kunihiro, Shigenaga Takatoshi, Uzumaki Tatsuya, Iwaki Masayo

    BIOCHEMISTRY   Vol. 46 ( 43 ) page: 12473-12481   2007.10

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    DOI: 10.1021/bi7008085

    Web of Science

  49. Protons bound to the Mn cluster in photosystem II oxygen evolving complex detected by proton matrix ENDOR Reviewed

    Yamada Hiroiku, Mino Hiroyuki, Itoh Shigeru

    BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS   Vol. 1767 ( 3 ) page: 197-203   2007.3

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    DOI: 10.1016/j.bbabio.2007.02.001

    Web of Science

  50. Function of chlorophyll d in reaction centers of photosystems I and II of the oxygenic photosynthesis of Acaryochloris marina Reviewed

    Itoh, S., Mino, H., Itoh, K., Shigenaga, T., Uzumaki, T. and Iwaki, M.

    Biochemistry   Vol. 46   page: 12473   2007

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  51. *Protons in the vicinity of Mn cluster in photosystem II oxygen evolving complex studied by proton matrix ENDOR Reviewed

    Yamada,H., Mino, H. and Itoh, S.

    Biochimica et Biophysica Acta     page: 1767,197-203   2007

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  52. ENDOR法の発展と応用 Invited Reviewed

    三野広幸

    分光研究   Vol. 55 ( 2 ) page: 110-119   2006.5

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  53. Thiocyanate hydrolase is a cobalt-containing metalloenzyme with a cysteine sulfinic acid ligand Reviewed

    Katayama,Y., Hashimoto,K., Nakayama, H., Mino,H. Nojiri,M. Ono,T. Nyunoya,H. Yohda,M. Takio,K. and Odaka,, M.

    J. Am. Chem. Soc.USA.   Vol. 128   page: 729-730   2006

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  54. g-Anisotropy of the S2-State Manganese Cluster in Single Crystals of Cyanobacterial Photosystem II Studied by W-Band EPR Spectroscopy" Reviewed

    Hideto Matsuoka, Ko Furukawa, Tatsuhisa Kato, Hiroyuki Mino, Jian-Ren Shen, Asako Kawamori

    The Journal of Physical Chemistry   Vol. 110   page: 13242-13247   2006

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  55. ESR signal of the iron-sulfer center FX and its function in the homodimeric reaction center of Heliobacterium modesticaldum Reviewed

    Miyamoto, R., Iwaki, M., Mino,H., Harada, J., Itoh, S. and Oh-oka, H.

    Biochemistry   Vol. 45   page: 6306-6316   2006

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  56. Efficient Peroxide Decoloration of C.I. Acid Orange 7 catalyzed by manganese Mesoporphyrin Dimer Derivatives in Micellar Solutions Reviewed

    Kondo,M., Kawashima, N.,Mitsui, T., Ito, S., Ishigure, S., Hashimoto, T.,Uemura, R., Dewa, T.,Yamashita, K., Mino,H., Itoh, S. and Nango, M.

    Chem.Soc.JPN.   Vol. 34   page: 1592-1593   2005

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  57. The Origin of the split signals in the Ca2+-depleted PS II; Photosynth.research Invited Reviewed

    Mino, H and Itoh, S.

    Photosynthesis Research   Vol. 84   page: 333-337   2005

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  58. *EPR properties of a g = 2 broad signal trapped in S1 state in the Ca2+-depleted Photosystem II Reviewed

    Mino, H and Itoh, S.

    Biochim.Biophys. Acta.   Vol. 1708   page: 42-49   2005

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  59. *Proton ENDOR spectroscopy of the primary donor P740+ made of a chlorophyll d special pair in photosystem I reaction center of Acaryochloris marina Reviewed

    Mino, H.,Kawamori,,A. Aoyama, D. Tomo,T. Iwaki M.and Itoh, S.

    Chem. Phys. Lett.   Vol. 411   page: 262-266   2005

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  60. Nanoscale Organization of Chlorophyll a in Mesoporous Silica: Efficient Energy Transfer and Stabilized Charge Separation as in Natural J. Phys. Chem. B (2004),13683 108, 13683-13687 Reviewed

    Itoh, T., Yano,K., Kajino, T., Itoh, S., Shibata, Y., Mino,H., Miyamoto, R., Inada, Satoshi Iwai, and Yoshiaki Fukushima

    J. Phys. Chem. B (2004)   Vol. 13683 ( 108 ) page: 13683-13687   2004

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  61. Nonlinear relationship between g=2 doublet and multiline signals in Ca2+-depleted Photosystem II Reviewed

    Mino, H., Ishii, A. and Ono, T.

    Biochimica et Biophysica Acta   Vol. 1606   page: 127-136   2003

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  62. Applications of pulsed ELDOR detected NMR measurements to studies of Photosystem: Magnetic characterization of YD tyrosine radical and Mn2+ bound to the high-affinity site Invited Reviewed

    Mino, H. and Ono, T.

    Applied Magnetic Resonance   Vol. 23   page: 571-584   2003

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  63. Photosystem I and II reaction centers in a new type oxygenic photosynthesis of Acaryochloris marina based on chlorophyll d: studies of delayed fluorescence and triplet state ESR Invited

    Itoh S, Fukushima Y, Itoh K, Maeda M, Mino H and Kumazaki S

    Journal of Photoscience   Vol. 9 ( 2 ) page: 70-73   2002

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  64. Spin relaxation of a spin-correlated radical pair P700+A1- in the PS I photosynthetic reaction center

    Dzuba, S. A., Kawamori, A., Katsuta, N., Hara, H., Mino, H. and Itoh, S.,

    Chem. Phys. Lett.   Vol. 362   page: 307-313   2002

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  65. Position of QA and ChlZ Relative to YZ and YD in Photosytem II Studied by Pulsed EPR Invited

    A. Kawamori, N. Katsuta, H. Mino, A. Ishii, J. Minagawa and T.-A. Ono

    J. Biol. Phys.   Vol. 28   page: 413-426   2002

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  66. Configuration of electron transfer cofactors in photosystem II studied by pulsed EPR Invited

    Kawamori A, Katsuta N, Arao S, Hara H, Mino H, Ishii A, Ono T, Minagawa J.

    Journal of Photoscience   Vol. 9 ( 2 ) page: 379-381   2002

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  67. Ca2+ Function in Photosynthetic Oxygen Evolution studied by Alkali Metal cation Substitution Reviewed

    Ono, T., Rompel, A., Mino, H. and Chiba, N.

    Biophysical Journal   Vol. 81   page: 1831-1840   2001

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  68. EPR studies of the water oxidizing complex in the S1 and the higher S-states: the manganese cluster and YZ radical Invited Reviewed

    Mino, H. and Kawamori, A.

    Biochimica et Biophysica Acta   Vol. 1503   page: 112-122   2001

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  69. An FTIR study on the structure of the oxygen-evolving Mn-cluster of Photosystem II in different spin forms of the S2 state Reviewed

    Onoda, K., Mino, H., Inoue, Y. and Noguchi, T.

    photosynthesis research   Vol. 63   page: 47-57   2000

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  70. Pulsed EPR studies of doublet signal and singlet-like signal in oriented Ca2+-depleted PS II membranes: location of the doublet signal center in PS II Reviewed

    Mino, H., Kawamori, A and Ono, T.

    Biochemistr   Vol. 39   page: 11034-11040   2000

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  71. pH-dependent characteristics of YZ radical in Ca2+depleted Photosystem II studied by CW EPR and pulsed ENDOR Reviewed

    Mino, H., Kawamori, A. and Ono, T.

    Biochimica et Biophysica Acta   Vol. 1457   page: 157-165   2000

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  72. Unique binding Site for Mn2+ ion responsible for Reducing an Oxidized YZ Tyrosine in Manganese-Depleted Photosystem II membranes Reviewed

    Ono, T., Mino, H

    Biochemistry   Vol. 38   page: 8778-8785   1999

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  73. Electron paramagnetic resonance study of the magnetic structure of the S1-statein oriented oxygen evolving photosystem membranes Reviewed

    Matsukawa, T., Kawamori, A.,Mino, H.

      Vol. 55   page: 895-901   1999

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  74. Dual-Mode EPR Study of New Signals from the S3-state of Oxygen-Evolving Complex in Photosystem II Reviewed

    Matsukawa, T., Mino H., Yoneda D.,Kawamori, A.

    biochemistry   Vol. 38   page: 4072-4077   1999

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  75. Light-induced high-spin signals from the oxygen evolving center (OEC) in Ca2+-depleted Photosystem II studied by dual mode EPR spectroscopy Reviewed

    Mino, H, Kawamori, A., Matsukawa, T. , Ono, T.

    Biochemistry   Vol. 37   page: 2794-2799   1998

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  76. An EPR and Pulsed ENDOR Study of the Structure of Tyrosine Z* in Mn-depleted Photosystem II.

    Mino, H., Astashkin, A.V., Kawamori, A.

    Spectrochim. Acta. Part A   Vol. 53   page: 1465-1483   1997

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  77. arallel Polarization Electron Paramagnetic resonance Studies of the S1-state Manganese Cluster in the Photosynthetic Oxygen-Evolving system.

    Yamauchi, T., Mino, H., Matsukawa, T., Kawamori, A. , Ono, T.

    Biochemistry   Vol. 36   page: 7520-7526   1997

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  78. Pulsed EPR study of S3' signal in the Ca2+-depleted Photosystem II Reviewed

    Astashkin, A.V. Mino, H., Kawamori, A. , Ono, T.

    Chem. Phys. Lett.   Vol. 272   page: 506-616   1997

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  79. pH and Temperature Dependence of Tyrosine Z* Decay Kinetics in Tris-Treated PS II Particles Studied by Time-Resolved EPR. Reviewed

    Shigemori, K., Mino, H., Kawamori, A.

    Biochim. Biophys. Acta   Vol. 1185   page: 213-220   1994

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  80. Microenvironments of tyrosine D+ and tyrosine Z+ in Photosystem II studied by proton matrix ENDOR Reviewed

    Mino, H., Kawamori, A.

    Biochim. Biophys. Acta   Vol. 1185   page: 213-220   1994

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  81. Matrix ENDOR of tyrosine D+ in oriented Photosystem II membranes Reviewed

    Mino, H., Satoh, J. Kawamori, A., Toriyama, K, Zimmermann, J-L.

    Biochim. Biophys. Acta   Vol. 1144   page: 426-433   1993

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▼display all

Books 5

  1. Analytical Techniques for the Elucidation of Protein Function

    Hiroyuki Mino( Role: Contributor ,  Chapter1)

    Wiley  2023.1  ( ISBN: 1119886325

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    Language:English Book type:Textbook, survey, introduction

  2. NMRによる有機材料分析とデータ解釈 事例集

    三野広幸( Role: Contributor ,  第3節 パルスESR法を用いたタンパク質の構造、相互作用解析)

    株式会社 技術情報協会  2021.9  ( ISBN:978-4-86104-860-9

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    Total pages:676   Responsible for pages:16   Language:Japanese Book type:Scholarly book

  3. 光合成のエネルギー変換と物質変換

    三野広幸( Role: Joint author)

    化学同人  2015 

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    Language:Japanese

  4. 光合成研究法(EPR法の項目)

    三野広幸( Role: Joint author)

    北海道大学低温科学研究所  2009.3 

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    Language:Japanese

    EPR法

  5. 光合成辞典 5項目執筆(

    三野広幸( Role: Sole author)

    学会出版センター  2003 

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    Language:Japanese Book type:Dictionary, encyclopedia

Presentations 37

  1. Structure of S2 High-Spin State Manganese Cluster of Photosystem Ⅱ by Multi-frequency Electron Paramagnetic Resonance (EPR) Spectroscopy

    Shinya Kosaki, Yoshiki Nakajima, Jian-Ren Shen, Hiroyuki Mino

    2024.3.18 

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    Event date: 2024.3

    Language:English   Presentation type:Oral presentation (general)  

    Country:Japan  

  2. Structure of S2 High-Spin State Manganese Cluster of PhotosystemⅡ by Q-band Pulsed Electron Paramagnetic Resonance (EPR) Spectroscopy

    2023.11.14 

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    Event date: 2023.11

    Language:English   Presentation type:Poster presentation  

    Country:Japan  

  3. ミトコンドリア電子移動鎖における磁場効果

    三野広幸, 西山侑希, 伊藤美佳子, 大野欽司

    第62回電子スピンサイエンス学会年会  2023.11.4  電子スピンサイエンス学会

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    Event date: 2023.11

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:神戸市   Country:Japan  

  4. Q-bandパルス電子常磁性共鳴(EPR)法による光化学系ⅡマンガンクラスターのS#D2#D2 High Spin状態の構造

    小崎慎也,三野広幸

    第62回電子スピンサイエンス学会年会  2023.11.2  電子スピンサイエンス学会

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    Event date: 2023.11

    Language:Japanese   Presentation type:Poster presentation  

    Venue:神戸大学   Country:Japan  

  5. Environments of Coordinates for High Spin S2 Intermediate in the Oxygen Evolving Complex Measured by Multi-frequency EPR

    Hiroyuki Mino, Shinya Kosaki, Yoshiki Nakajima, Jian-Ren Shen

    2023.3.15 

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    Event date: 2023.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  6. g = 5状態の配位子場対称性のゆがみ

    三野広幸, 小崎慎也,沈建仁

    第60回電子スピンサイエンス学会年会  2022.12.4  電子スピンサイエンス学会

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    Event date: 2022.12

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:熊本   Country:Japan  

  7. PELDOR法により決定した光化学系Ⅱの2つのMn2+親和サイト

    三野 広幸,浅田瑞枝

    第60回生物物理学会年会  2022.9.29  日本生物物理学会

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    Event date: 2022.9

    Language:English   Presentation type:Poster presentation  

    Venue:函館   Country:Japan  

  8. High Spin Structures of S2 State Manganese Cluster International conference

    Hiroyuki Mino、Shinya Kosaki

    18th international congress on photosynthesis research  2022.8  International society of photosynthesis research

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    Event date: 2022.7 - 2022.8

    Language:English   Presentation type:Poster presentation  

    Venue:Brisbane   Country:Australia  

  9. 酸素発生系中間状態 g = 5 S2状態の熱的安定性

    三野 広幸

    第59回生物物理学会年会  2021.11  日本生物物理学会

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    Event date: 2021.11

    Language:English   Presentation type:Oral presentation (general)  

    Venue:東北大学   Country:Japan  

  10. Molecular Structure of the S2 State with a g = 5 Signal in the Oxygen Evolving Complex of Photosystem II International conference

    Mino, H.

    22nd International Society of Magnetic Resonance Conference 9th Asai-Pacific NMR Symposium (ISMAR -APNMR)  2021.8  ISMAR-APNMR2021 local organizing committee

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    Event date: 2021.8

    Language:English   Presentation type:Oral presentation (general)  

    Venue:OSAKA   Country:Japan  

  11. Electron Transfer on for Tyrosine YZ in Plant Photosystem II Detected by High Frequency Modulation International conference

    Sumi,T. and Mino, H

    22nd International Society of Magnetic Resonance Conference 9th Asai-Pacific NMR Symposium (ISMAR -APNMR)   2021.8  ISMAR-APNMR2021 local organizing committee

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    Event date: 2021.8 - 0201.8

    Language:English   Presentation type:Poster presentation  

    Venue:OSAKA   Country:Japan  

  12. g~5 EPR 信号をもつ酸素発生系S2中間状態の形成 International coauthorship

    三野広幸, 田口翔太, Liangliang Shen, Guangye Han, 梅名泰史, 沈建仁, 野口巧

    第62回日本植物生理学会年会  2021.3.14 

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    Event date: 2021.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue: 島根大学(オンライン)  

  13. g=5 をもつ酸素発生系マンガンクラスターの分子構造 Invited

    三野 広幸, 田口 翔大, , 野口 巧

    第58回電子スピンサイエンス学会年会  2020.11.13 

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    Event date: 2020.11

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Venue:東北大学(オンライン)  

  14. 光合成酸素発生系におけるg=5 S2状態の分子構造

    三野 広幸, 田口 翔大,, 野口 巧

    第59回生物物理学会年会  2020.9.16 

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    Event date: 2020.9

    Language:English   Presentation type:Poster presentation  

    Venue: 群馬大学(オンライン)  

  15. 光合成酸素発生系高スピンS2状態のEPR信号の起源

    三野 広幸,長嶋宏樹

    日本植物生理学会年会  2020.3 

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    Event date: 2020.3

    Venue:大阪大学 (covid19対策により中止)  

  16. パルスESR法を用いたタンパク質間相互作用及び反応中心の解明 Invited

    三野 広幸

    第57回電子スピンサイエンス学会年会  2019.11.9 

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    Event date: 2019.11

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Venue:川崎市コンベンションホール  

  17. 光化学系Ⅱの表在性タンパク質による水分解Mn4CaO5クラスターのS2構造異性体平衡の制御機構

    田口 翔大, Shen Liangliang, Han Guangye, 沈 建仁, 野口 巧, 三野 広幸

    第57回生物物理学会年会  2019.9 

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    Event date: 2019.9

    Language:English   Presentation type:Poster presentation  

    Venue:宮崎県シーガイアコンベンションセンター  

  18. 機能性タンパク質Photozipperの構造と反応

    三野広幸, 佃野弘幸, 小関康平, 久冨修

    第46回生体分子科学討論会  2019.6.22 

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    Event date: 2019.6

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:筑波大学  

  19. 青色光により誘発される機能性タンパク質Photozipperの構造変化

    三野 広幸, 佃野弘幸,小関 康平,小林 樹, 久冨 修

    第56回電子スピンサイエンス学会年会  2018.11 

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    Event date: 2018.11

    Language:English   Presentation type:Oral presentation (general)  

    Venue:東京工業大学  

  20. Function of Blue Sensor Protein Photozipper Investigated by Pulsed EPR Invited International conference

    Hiroyuki Mino, Hiroyuki Tsukuno Kouhei Ozeki, Hiroki Nagashima, Itsuki Kobayashi and Osamu Hisatomi

    APES-IES2018 The 3rd joint conference of the EPR/ESR Societies  2018.9 

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    Event date: 2018.9

    Language:English   Presentation type:Oral presentation (invited, special)  

    Venue:Brisbane   Country:Australia  

  21. Ca2+除去した光化学系Ⅱの高酸化状態でのスピン構造

    酒井貴弘,三野 広幸

    第56回生物物理学会年会  2018.9 

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    Event date: 2018.9

    Language:English   Presentation type:Oral presentation (general)  

    Venue:岡山大学  

  22. 光制御型転写因子Photozipperのラジカル形成と構造

    佃野 弘幸、小関 康平、小林樹、久冨 修、三野 広幸

    第22回ESRフォーラム研究会  2018.7.21  第22回ESRフォーラム研究会

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    Event date: 2018.7

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:同支社女子大学  

  23. Ca2+除去マンガンクラスターのスピン構造

    酒井貴弘,三野 広幸

    第45回生体分子科学討論会  2018.6 

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    Event date: 2018.6

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:大阪市立大学  

  24. 光化学系IIの熱損傷の分子機構

    寺島 尚貴, 佃野 弘幸, 三野 広幸

    日本植物生理学会年会  2018.3 

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    Event date: 2018.3

    Language:English   Presentation type:Oral presentation (general)  

    Venue:札幌コンベンションセンター  

  25. 機能性タンパク質Photozipperのラジカル形成と構造変化

    三野広幸, 佃野弘幸, 小関康平, 久冨修

    第77回日本物理学会  2018.3 

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    Event date: 2018.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:東京理科大学  

  26. Mnクラスター近傍水分子のS状態に依存した状態変化

    山田比呂育,三野広幸,伊藤繁

    第47回日本植物生理学会 

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    Event date: 2006.3

    Language:Japanese   Presentation type:Poster presentation  

    Country:Japan  

  27. シアノバクテリアAcaryochloris marinaの新型光合成反応中心の機能

    三野広幸,河盛阿佐子,青山大輔,鞆達也,岩城雅代,長井浩子, 内田小百合,伊藤繁

    電子スピンサイエンス学会 

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    Event date: 2005.10

    Language:Japanese   Presentation type:Poster presentation  

    Country:Japan  

  28. 電子核二重共鳴(ENDOR)法による光化学系Ⅰ反応中心 クロロフィルの電子状態の解析

    三野広幸,河盛阿佐子,青山大輔,鞆達也,岩城雅代,伊藤繁

    第32回生体分子化学討論会 

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    Event date: 2005.6

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  29. ENDOR法による光化学系Ⅰ反応中心クロロフィル P700+とP740+の電子状態の解析

    三野広幸,伊藤繁

    日本植物生理学会46回年会 

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    Event date: 2005.3

    Language:Japanese   Presentation type:Poster presentation  

    Country:Japan  

  30. Split signalとは何だったのか?

    三野広幸,伊藤繁

    生物物理学会42回年会 

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    Event date: 2004.12

    Language:Japanese   Presentation type:Poster presentation  

    Country:Japan  

  31. 光合成酸素発生系マンガンクラスターと相互作用信号の起源

    三野広幸,伊藤繁

    電子スピンサイエンス学会 

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    Event date: 2004.11

    Language:Japanese   Presentation type:Poster presentation  

    Country:Japan  

  32. Origin of the g=2 broad EPR signal in the Ca2+-depleted PSII in S1 state

    mino,h. and Itoh, S

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    Event date: 2004.8

    Language:English   Presentation type:Poster presentation  

  33. High frequency EPR of the Mn-cluster in single crystals of cyanobactreial phtosystem II

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    Event date: 2004.8

    Language:English   Presentation type:Oral presentation (invited, special)  

  34. The relevance of the split signals in the Ca2+-depleted PS II International conference

    From Biophysics to Molecular Biology, a Path in the Research of Photosystem II 

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    Event date: 2004.8

    Language:English   Presentation type:Poster presentation  

  35. Ca2+除去試料S1-S2状態遷移の中間体 ESR信号の観測

    第45回日本植物生理学会年会 

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    Event date: 2004.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  36. 光合成マンガンクラスターS2状態生成機構の研究

    三野広幸,伊藤繁

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    Event date: 2003.10

    Language:Japanese   Presentation type:Poster presentation  

    Country:Japan  

  37. 酸素発生系S2状態の形成メカニズムの研究

    三野広幸,伊藤繁

    日本生物物理学会第41回年会 

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    Event date: 2003

    Language:Japanese   Presentation type:Poster presentation  

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Research Project for Joint Research, Competitive Funding, etc. 2

  1. パルスESR法による新型光合成バクテリア光化学系Ⅰの電子移動解析

    2007.9 - 2008.8

    実吉奨学会 

    三野広幸

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    Grant type:Competitive

    Grant amount:\1000000 ( Direct Cost: \1000000 )

  2. ENDOR法による光合成酸素発生マンガン錯体の基質水分子分 解機構の解明

    2007

    倉田記念日立科学技術財団 

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    Grant type:Competitive

    Grant amount:\1000000 ( Direct Cost: \1000000 )

KAKENHI (Grants-in-Aid for Scientific Research) 10

  1. 光合成酸素発生系の基質水分子結合過程の追跡

    Grant number:22K06160  2022.4 - 2025.3

    科学研究費助成事業  基盤研究(C)

    三野 広幸

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    Authorship:Principal investigator  Grant type:Competitive

    Grant amount:\4160000 ( Direct Cost: \3200000 、 Indirect Cost:\960000 )

    光合成酸素発生反応は光化学系Ⅱタンパク質のマンガンクラスターで行われる。反応中間状態の分子構造はX線結晶構造解析により構造が解明されつつあるが、分子構造の解明だけでは酸素発生メカニズムは解明できない。本研究はマンガンクラスターの高酸化中間状態S2、S3の電子状態を電子スピン共鳴(ESR)により解析し、基質酸素原子の取り込み過程を解明することを目的としている。申請者が発見した新しいS2中間状態g =5異性体は高スピン(S = 5/2 or 7/2)を持ち酸素原子取り込み反応に直結する構造をとると考えられる。本研究ではこの信号の解析を中心に高酸化状態の解明を目指す。

  2. Molecular Structure of Intermediates in Oxygen Evolving Complex with Spin Conversion

    Grant number:20H05096  2020.4 - 2022.3

    Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

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    Authorship:Principal investigator 

    Grant amount:\2600000 ( Direct Cost: \2000000 、 Indirect Cost:\600000 )

  3. 非選択励起多周波混合パルスEPR法によるタンパク質の構造解明

    Grant number:26620003  2014.4 - 2017.3

    科学研究費補助金 

    三野広幸

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    Authorship:Principal investigator 

  4. 植物光合成の光化学系IIにおける初期電荷分離状態の立体構造

    Grant number:25288004  2013.4 - 2016.3

    文部科学省  科学研究費補助金  基盤研究(B)

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    Authorship:Coinvestigator(s) 

  5. 酸素発生反応を安定化させる光化学系II表在性タンパク質の構造・機能・進化

    Grant number:24370025   2012.4 - 2015.3

    科学研究費補助金  基盤研究(B)

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    Authorship:Coinvestigator(s) 

  6. 生体膜を用いた量子テレポーテーション過程の追跡

    Grant number:22654051  2010.4 - 2013.3

    科学研究費補助金  挑戦的萌芽研究,課題番号:22654051

    三野 広幸

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    Authorship:Principal investigator 

  7. ラジカル対機構がもたらす青色光受容センサータンパク質の動作原理の解明

    Grant number:21370069   2009.4 - 2012.3

    科学研究費補助金  基盤研究(B),課題番号:21370069

    三野 広幸

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    Authorship:Principal investigator 

  8. 光化学系IIMnクラスターによる光合成水分解反応ケミストリーの研究

    Grant number:19370061  2007.3 - 2011.4

    文部科学省  科学研究費補助金  基盤研究(B)

    三野広幸

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    Authorship:Coinvestigator(s)  Grant type:Competitive

  9. クロロフィルd導入による低エネルギー新型光合成の作成:光合成原理の解明

    Grant number:19370064  2007.3 - 2009.4

    文部科学省  科学研究費補助金  基盤研究(B)

    三野広幸

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    Authorship:Coinvestigator(s) 

  10. パルスESRによる光合成タンパク質電子移動反応の対称性の解析

    Grant number:17740222  2005.4 - 2007.3

    科学研究費補助金  若手研究(B),課題番号:17740222

    三野 広幸

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    Authorship:Principal investigator 

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Teaching Experience (On-campus) 31

  1. 先端物理学特論

    2021

  2. 数理物理学Ⅰ演習・解析力学演習

    2021

  3. 基礎セミナーB

    2021

  4. 光生体エネルギー講究3

    2021

  5. 光生体エネルギー講究2

    2021

  6. 光生体エネルギー講究3

    2020

  7. 先端物理学特論

    2020

  8. 基礎セミナーB

    2019

  9. 物理学演習

    2019

  10. 光生体エネルギー講究3

    2019

  11. 光生体エネルギー講究2

    2019

  12. 先端物理学特論

    2019

  13. 基礎セミナーB

    2018

  14. 物理学演習

    2018

  15. 物理学演習

    2018

  16. 光生体エネルギー講究3

    2018

  17. 光生体エネルギー講究2

    2018

  18. 物理学演習

    2017

  19. 基礎セミナーB

    2017

  20. 物理学演習

    2016

  21. 物理学実験

    2016

  22. 物質と科学

    2015

  23. 物理学演習

    2015

  24. 物質と科学

    2014

  25. 物理学演習

    2014

  26. 基礎セミナーA

    2013

  27. 物理学演習

    2013

  28. 基礎セミナーA

    2012

  29. 物理学演習

    2012

  30. First Year Seminar A

    2011

  31. 物理学演習

    2011

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Teaching Experience (Off-campus) 3

  1. 化学特論1、基礎化学特論1a

    2022.9 Saitama University)

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    Level:Postgraduate  Country:Japan

  2. 化学特別講義3

    2020.11 Kyoto University)

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    Level:Postgraduate  Country:Japan

  3. 2008.4 - 2009.3 静岡大学)

 

Social Contribution 6

  1. 電子スピンでみる生体の構造と機能

    Role(s):Advisor, Informant

    愛知県立岡崎高等学校、あいち科学技術教育推進協議会  科学三昧inあいち2023  2023.12

  2. 理学とは何か

    Role(s):Lecturer

    岐阜県立大垣北高校  模擬授業  2023.9

  3. SSH発表会

    Role(s):Lecturer

    一宮高校   SSH発表会  名古屋大学  2023.8

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    Audience: High school students

    Type:Visiting lecture

    一宮高校SSHによる近隣高校を含めた研究発表会

  4. 理学とは何だろう

    Role(s):Lecturer

    愛知淑徳高校  2021.6

  5. 理学とはなんだろう

    Role(s):Lecturer

    愛知淑徳高校  模擬授業  2021.6

  6. 名大研究室の扉

    Role(s):Lecturer

    名古屋大学 河合塾  2018.8

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