Faculty Profiles - MIYATA, Takaki

Papers - MIYATA, Takaki

Division display 21 - 40 of about 131 ／ All the affair displays >>

Actin-binding protein filamin-A drives tau aggregation and contributes to progressive supranuclear palsy pathology. Reviewed

Tsujikawa K, Hamanaka K, Riku Y, Hattori Y, Hara N, Iguchi Y, Ishigaki S, Hashizume A, Miyatake S, Mitsuhashi S, Miyazaki Y, Kataoka M, Jiayi L, Yasui K, Kuru S, Koike H, Kobayashi K, Sahara N, Ozaki N, Yoshida M, Kakita A, Saito Y, Iwasaki Y, Miyashita A, Iwatsubo T, Japanese Alzheimer’s Disease Neuroimaging Initiative (J-ADNI), Ikeuchi T, Japanese Longitudinal Biomarker Study in PSP and CBD (JALPAC) Consortium, Miyata T, Sobue G, Matsumoto N, Sahashi K, Katsuno M

Science advances Vol. 8 ( 21 ) page： eabm5029 2022.5

Language：English Publisher：Science Advances

While amyloid-β lies upstream of tau pathology in Alzheimer’s disease, key drivers for other tauopathies, including progressive supranuclear palsy (PSP), are largely unknown. Various tau mutations are known to facilitate tau aggregation, but how the nonmutated tau, which most cases with PSP share, increases its propensity to aggregate in neurons and glial cells has remained elusive. Here, we identified genetic variations and protein abundance of filamin-A in the PSP brains without tau mutations. We provided in vivo biochemical evidence that increased filamin-A levels enhance the phosphorylation and insolubility of tau through interacting actin filaments. In addition, reduction of filamin-A corrected aberrant tau levels in the culture cells from PSP cases. Moreover, transgenic mice carrying human filamin-A recapitulated tau pathology in the neurons. Our data highlight that filamin-A promotes tau aggregation, providing a potential mechanism by which filamin-A contributes to PSP pathology.

DOI： 10.1126/sciadv.abm5029

Scopus

PubMed

Embryonic Pericytes Promote Microglial Homeostasis and Their Effects on Neural Progenitors in the Developing Cerebral Cortex. Reviewed

Hattori Y, Itoh H, Tsugawa Y, Nishida Y, Kurata K, Uemura A, Miyata T

Journal of Neuroscience Vol. 42 ( 3 ) page： 362 - 376 2022.1

Authorship：Last author Language：English Publisher：The Journal of neuroscience : the official journal of the Society for Neuroscience

Multifaceted microglial functions in the developing brain, such as promoting the differentiation of neural progenitors and contributing to the positioning and survival of neurons, have been progressively revealed. Although previous studies have noted the relationship between vascular endothelial cells and microglia in the developing brain, little attention has been given to the importance of pericytes, the mural cells surrounding endothelial cells. In this study, we attempted to dissect the role of pericytes in microglial distribution and function in developing mouse brains. Our immunohistochemical analysis showed that approximately half of the microglia attached to capillaries in the cerebral walls. Notably, a magnified observation of the position of microglia, vascular endothelial cells and pericytes demonstrated that microglia were preferentially associated with pericytes that covered 79.8% of the total capillary surface area. Through in vivo pericyte depletion induced by the intraventricular administration of a neutralizing antibody against platelet-derived growth factor receptor (PDGFR)β (clone APB5), we found that microglial density was markedly decreased compared with that in control antibody-treated brains because of their low proliferative capacity. Moreover, in vitro coculture of isolated CD11b+ microglia and NG2+PDGFRα- cells, which are mostly composed of pericytes, from parenchymal cells indicated that pericytes promote microglial proliferation via the production of soluble factors. Furthermore, pericyte depletion by APB5 treatment resulted in a failure of microglia to promote the differentiation of neural stem cells into intermediate progenitors. Taken together, our findings suggest that pericytes facilitate microglial homeostasis in the developing brains, thereby indirectly supporting microglial effects on neural progenitors.SIGNIFICANCE STATEMENT This study highlights the novel effect of pericytes on microglia in the developing mouse brain. Through multiple analyses using an in vivo pericyte depletion mouse model and an in vitro coculture study of isolated pericytes and microglia from parenchymal cells, we demonstrated that pericytes contribute to microglial proliferation and support microglia in efficiently promoting the differentiation of neural stem cells into intermediate progenitors. Our present data provide evidence that pericytes function not only in the maintenance of cerebral microcirculation and blood brain barrier (BBB) integrity but also in microglial homeostasis in the developing cerebral walls. These findings will expand our knowledge and help elucidate the mechanism of brain development both in healthy and disease conditions.

DOI： 10.1523/JNEUROSCI.1201-21.2021

Scopus

PubMed

Developmentally interdependent stretcher-compressor relationship between the embryonic brain and the surrounding scalp in the preosteogenic head. Reviewed

Tsujikawa K, Saito K, Nagasaka A, Miyata T

Developmental Dynamics 2022.1

Authorship：Last author,　Corresponding author Language：English Publisher：Developmental Dynamics

Background: How developing brains mechanically interact with the surrounding embryonic scalp layers (ie, epidermal and mesenchymal) in the preosteogenic head remains unknown. Between embryonic day (E) 11 and E13 in mice, before ossification starts in the skull vault, the angle between the pons and the medulla decreases, raising the possibility that when the elastic scalp is directly pushed outward by the growing brain and thus stretched, it recoils inward in response, thereby confining and folding the brain. Results: Stress-release tests showed that the E11-13 scalp recoiled and that the in vivo prestretch prerequisite for this recoil was physically dependent on the brain (pressurization at 77-93 Pa) and on actomyosin and elastin within the scalp. In scalp-removed heads, brainstem folding was reduced, and the spreading of ink from the lateral ventricle to the spinal cord that occurred in scalp-intact embryos (with >5 μL injection) was lost, suggesting roles of the embryonic scalp in brain morphogenesis and cerebrospinal fluid homeostasis. Under nonstretched conditions, scalp cell proliferation declined, while the restretching of the shrunken scalp rescued scalp cell proliferation. Conclusions: In the embryonic mouse head before ossification, a stretcher-compressor relationship elastically develops between the brain and the scalp, underlying their mechanically interdependent development.

DOI： 10.1002/dvdy.451

Scopus

PubMed

Comparison of the Mechanical Properties Between the Convex and Concave Inner/Apical Surfaces of the Developing Cerebrum Reviewed

Frontiers in Cell and Developmental Biology Vol. 9 2021.7

A mouse model of microglia-specific ablation in the embryonic central nervous system. Reviewed

Li C, Konishi H, Nishiwaki K, Sato K, Miyata T, Kiyama H

Neuroscience Research 2021.6

Language：English Publisher：Neuroscience Research

Microglia, which migrate into the central nervous system (CNS) during the early embryonic stages, are considered to play various roles in CNS development. However, their embryonic roles are largely unknown, partly due to the lack of an effective microglial ablation system in the embryo. Here, we show a microglial ablation model by injecting diphtheria toxin (DT) into the amniotic fluid of Siglechdtr mice, in which the gene encoding DT receptor is knocked into the microglia-specific gene locus Siglech. We revealed that embryonic microglia were depleted for several days throughout the CNS, including some regions where microglia transiently accumulated, at any embryonic time point from embryonic day 10.5, when microglia colonize the CNS. This ablation system was specific for microglia because CNS-associated macrophages, which are a distinct population from microglia that reside in the CNS interfaces such as meninges, were unaffected. Therefore, this microglial ablation system is highly effective for studying the embryonic functions of microglia.

DOI： 10.1016/j.neures.2021.06.002

Scopus

PubMed

大脳皮質の発生における力学 Invited

宮田卓樹

Vol. 38 page： 1510 - 1512 2020.12

Two-photon microscopic observation of cell-production dynamics in the developing mammalian neocortex in utero. Invited Reviewed

Kawasoe R, Shinoda T, Hattori Y, Nakagawa M, Pham TQ, Tanaka Y, Sagou K, Saito K, Katsuki S, Kotani T, Sano A, Fujimori T, Miyata T.

Development, Growth & Differentiation Vol. 62 page： 118 - 128 2020.1

Meflin-positive cancer-associated fibroblasts inhibit pancreatic carcinogenesis Invited Reviewed International coauthorship

Mizutani Y, Kobayashi H, Iida T, Asai N, Masamune A, Hara A, Esaki N, Ushida K, Mii S, Shiraki Y, Ando K, Weng L, Ishihara S, Ponik SM, Conklin MH, Haga H, Nagasaka A, Miyata T, Matsuyama M, Kobayashi T, Fujii T, Yamada S, Yamaguchi J, Wang T, Woods SL, Worthley D, Shimamura T, Fujishiro M, Hirooka Y, Takahashi M, and Enomoto A

Cancer Research Vol. 79 page： 5367 - 5381 2019.10

Roles of the Mesenchymal Stromal/Stem Cell Marker Meflin in Cardiac Tissue Repair and the Development of Diastolic Dysfunction

Hara Akitoshi, Kobayashi Hiroki, Asai Naoya, Saito Shigeyoshi, Higuchi Takahiro, Kato Katsuhiro, Okumura Takahiro, Bando Yasuko K., Takefuji Mikito, Mizutani Yasuyuki, Miyai Yuki, Saito Shoji, Maruyama Shoichi, Maeda Keiko, Ouchi Noriyuki, Nagasaka Arata, Miyata Takaki, Mii Shinji, Kioka Noriyuki, Worthley Daniel L., Murohara Toyoaki, Takahashi Masahide, Enomoto Atsushi

CIRCULATION RESEARCH Vol. 125 ( 4 ) page： 414-430 2019.8

Lzts1 controls both neuronal delamination and outer radial glial-like cell generation during mammalian cerebral development Reviewed

Kawaue T., Shitamukai A., Nagasaka A., Tsunekawa Y., Shinoda T., Saito K., Terada R., Bilgic M., Miyata T., Matsuzaki F., Kawaguchi A.

NATURE COMMUNICATIONS Vol. 10 2019.6

[Production Logistics and Crowd Dynamics in the Developing Cerebral Cortex].

Miyata T

Brain and nerve = Shinkei kenkyu no shinpo Vol. 71 ( 4 ) page： 415-421 2019.4

大脳皮質形成の生産物流とクラウドダイナミクス Invited Reviewed

宮田卓樹

Vol. 71 page： 415 - 421 2019.4

Novel Development of Diabetic Retinopathy Research by Integrated Approach Reviewed

植村明嘉, 小椋俊太郎, 小椋俊太郎, 倉田薫里, 小峯祐美, 大矢恵, 井上奈緒美, 井上奈緒美, 小椋祐一郎, 高瀬弘嗣, 宮田卓樹, 福嶋葉子, 楠原仙太郎, 平島正則, 池田わたる, 南敬, 渋谷正史, 吉田富, MANN Fanny, 酒井秀人, 奈良裕美, YANCOPOULOS George D, WIEGAND Stanley J, KIM Pilhan, KOH Gou Young, 西川伸一

日本眼科学会雑誌 Vol. 123 ( 3 ) page： 312 - 336 2019

神経幹細胞による「他力」活用：集団的核移動の効率と細胞産生の安全 Invited Reviewed

篠田友靖，川上巧，宮田卓樹

Vol. 90 ( 6 ) page： 820 - 824 2018.12

Embryonic Neocortical Microglia Express Toll-Like Receptor 9 and Respond to Plasmid DNA Injected into the Ventricle: Technical Considerations Regarding Microglial Distribution in Electroporated Brain Walls.

Hattori Y, Miyata T

eNeuro Vol. 5 ( 6 ) 2018.11

Microglia extensively survey the developing cortex via the CXCL12/CXCR4 system to help neural progenitors to acquire differentiated properties.

Hattori Y, Miyata T

Genes to cells : devoted to molecular & cellular mechanisms 2018.8

Role of extrinsic mechanical force in the development of the RA-I tactile mechanoreceptor

Trung Quang Pham, Kawaue Takumi, Hoshi Takayuki, Tanaka Yoshihiro, Miyata Tataki, Sano Akihito

SCIENTIFIC REPORTS Vol. 8 2018.7

Synaptic transmission from subplate neurons controls radial migration of neocortical neurons

Ohtaka-Maruyama Chiaki, Okamoto Mayumi, Endo Kentaro, Oshima Minori, Kaneko Noe, Yura Kei, Okado Haruo, Miyata Takaki, Maeda Nobuaki

SCIENCE Vol. 360 ( 6386 ) page： 313-316 2018.4

Radial Glial Fibers Promote Neuronal Migration and Functional Recovery after Neonatal Brain Injury Reviewed

Jinnou H, Sawada M, Kawase K, Kaneko N, Herranz-Pérez V, Miyamoto T, Kawaue T, Miyata T, Tabata Y, Akaike T, García-Verdugo JM, Ajioka I, Saitoh S, Sawamoto K.

Cell Stem Cell. Vol. 22 ( 1 ) page： 128–137.e9 2018.1

Neural Progenitor Cells Undergoing Yap/Tead-Mediated Enhanced Self-Renewal Form Heterotopias More Easily in the Diencephalon than in the Telencephalon. Reviewed

Saito K, Kawasoe R, Sasaki H, Kawaguchi A, Miyata T.

Neurochemical Research Vol. 43 ( 1 ) page： 171–180 2018.1

　 PREV - NEXT 　

<MIYATA, Takaki>

Papers - MIYATA, Takaki