Updated on 2026/09/02

写真a

 
SEI Haruka
 
Organization
Graduate School of Bioagricultural Sciences Department of Applied Biosciences Assistant Professor
Graduate School
Graduate School of Bioagricultural Sciences
Undergraduate School
School of Agricultural Sciences Department of Applied Biosciences
Title
Assistant Professor

Degree 1

  1. 博士（農学） （ 2026.3   名古屋大学 ） 

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Research History 3

  1. Nagoya University   Graduate School of Bioagricultural Sciences Department of Applied Biosciences   Assistant Professor

    2026.9

  2. Nagoya University   Graduate School of Bioagricultural Sciences   Postdoctoral Researcher

    2026.4 - 2026.8

  3. Japan Society for the Promotion of Science   Research Fellowship for Young Scientists DC2

    2024.4 - 2026.3

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Education 3

  1. Nagoya University   Graduate School of Bioagricultural Sciences   Department of Applied Biosciences

    2023.4 - 2026.3

  2. Nagoya University   Graduate School of Bioagricultural Sciences   Department of Applied Biosciences

    2021.4 - 2023.3

  3. Nagoya University   School of Agricultural Sciences   Department of Applied Biosciences

    2017.4 - 2021.3

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Professional Memberships 4

  1. 日本酸化ストレス学会

    2026.4

  2. 日本農芸化学会

    2024.3

  3. 日本生化学会

    2022.4

  4. 日本細胞外小胞学会

    2022.4

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Awards 5

  1. グランプリ Nagoya University 3 Minute Thesis competition

    2025.10   名古屋大学  

  2. 名古屋大学学術奨励賞

    2025.5   名古屋大学  

  3. 優秀ポスター発表賞

    2025.2   日本酸化ストレス学会東海支部第13回学術集会  

  4. 奨励賞

    2024.5   第88回日本生化学会中部支部例会  

  5. 中部支部企業奨励賞

    2022.10   日本農芸化学会中部支部第193回例会  

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Papers 8

  1. Adductome-Based Identification of Novel Oxidized Phospholipid-Lysine Adducts Formed in Low-Density Lipoprotein Reviewed International journal

    Fumie Nakashima, Yuto Otsuka, Takuo Aoyama, Naoya Hirade, Haruka Sei, Hiroyuki Hattori, Ryosuke Kikuchi, Tadashi Matsushita, Ken-Ichi Yamada, Koji Uchida, Takahiro Shibata

    Biochemistry     2026.8

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：American Chemical Society (ACS)  

    Abstract

    The oxidative modification of low-density lipoprotein (LDL) is involved in the generation of lipid peroxidation-derived electrophilic aldehydes, which covalently react with apolipoprotein B-100 to form various adducts. Although the oxidative modification of LDL is critical in the pathogenesis of atherosclerosis, the structural properties of adducts in oxidized LDL (oxLDL) remain unclear. Thus, we aimed to characterize oxLDL using the adductome approach, a comprehensive mass spectrometry-based analysis designed to detect specific product ions from positively ionized oxidized phosphatidylcholine (oxPC) adducts. The amounts of several adducts, including four major oxPC-lysine (oxPC-Lys) adducts, prominently increased when LDL was oxidized with Cu2+. Analysis of the synthetic adduct candidates through mass spectrometry revealed two oxPC-Lys Schiff base adducts and two novel oxPC-Lys amide-type adducts as the major lysine adducts in oxLDL. The oxPC-Lys Schiff base adducts could be transformed into stable amide-type adducts during LDL oxidation. The amounts of oxPC-Lys adducts were significantly higher in the sera of hyperlipidemic mice than in the sera of control mice. The recognition of oxPC-Lys adducts by macrophages suggested that these adducts were involved in the phagocytosis of oxLDL by macrophages. These findings provide insights into the structural properties of lipoproteins modified under oxidative stress and their biological implications. Moreover, this study is the first to identify and quantify oxPC-Lys amide-type adducts in vitro and in vivo.

    DOI： 10.1021/acs.biochem.6c00425

    Other Link： https://pubs.acs.org/bichaw/article-pdf/doi/10.1021/acs.biochem.6c00425/66523242/acs.biochem.6c00425.pdf

  2. Proteomic profiling identifies circulating soluble MCAM/CD146 as a candidate biomarker for abnormal semen parameters in male infertility Reviewed International journal

    Ryosuke Kikuchi, Takahiro Shibata, Masahiro Nakatochi, Haruka Sei, Yumiko Kobayashi, Hiroyasu Kidoya, Fumitaka Muramatsu, Naomi Furusawa, Keiko Kano, Emi Mishiro-Sato, Hidekazu Ishida, Yasuo Katagiri, Atsuo Suzuki, Hirokazu Matsubara, Kyohei Takano, Tatsuro Furui, Hiroaki Kajiyama, Hiroyuki Okura

    Annals of Clinical Biochemistry: International Journal of Laboratory Medicine     2026.5

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    Publishing type：Research paper (scientific journal)   Publisher：SAGE Publications  

    Background

    A growing body of evidence suggests that male infertility is a precursor to future health problems. This study aimed to develop a unique circulating biomarker that could contribute to the diagnosis of male infertility.

    Materials and methods

    The study included 114 adult male participants aged 20–50 years who underwent sperm collection for in vitro fertilization and embryo transfer at Nagoya University Hospital, Gifu University Hospital, and Misao Ladies Hospital.

    Results

    Circulating VEGF-A and sFlt-1 levels did not differ significantly between men with normal and abnormal semen parameters. In contrast, proteomic analysis identified VEGFR1/2-related proteins associated with sperm motility, among which soluble MCAM/CD146 was significantly elevated in participants with abnormal semen parameters (84.0 [20.9–183.8] pg/mL vs 352.7 [115.8–1236.3] pg/mL, p < 0.0001), and in similar those with reduced sperm motility (106.5 [25.1–282.6] pg/mL vs 352.7 [125.5–1133.4] pg/mL, p < 0.0001). Receiver operating characteristic analysis demonstrated the diagnostic potential of soluble MCAM/CD146, with an area under the curve of 0.787, 66.7% sensitivity, and 78.6% specificity at the optimal threshold, supporting its role as a candidate biomarker for male infertility.

    Conclusions

    Circulating soluble MCAM/CD146 may represent a potential biomarker associated with abnormal semen parameters.

    DOI： 10.1177/00045632261454320

    Other Link： https://journals.sagepub.com/doi/full-xml/10.1177/00045632261454320

  3. A missense mutation in acyl-CoA synthetase ACSL4 reveals essential residues for catalytic activity in ferroptosis Reviewed International journal Open Access

    Haruka Sei, Harumi Ando, Fumie Nakashima, Terunao Takahara, Masaki Kita, Ken-ichi Yamada, Koji Uchida, Takahiro Shibata

    Journal of Biological Chemistry   Vol. 302 ( 3 ) page： 111232 - 111232   2026.3

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    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Elsevier BV  

    DOI： 10.1016/j.jbc.2026.111232

    Open Access

  4. Oral administration of pomegranate juice enhances antioxidant defense and reduces tyrosinase activity in mouse skin Reviewed International journal Open Access

    Yuki Washiya, Haruka Sei, Fumie Nakashima, Takahiro Shibata, Tsuchiyoshi Fujino

    Food Science and Technology Research     2026

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    Publishing type：Research paper (scientific journal)   Publisher：Japanese Society for Food Science and Technology  

    DOI： 10.3136/fstr.fstr-d-26-00010

    Open Access

  5. Structural and Functional Basis of G Protein-Coupled Receptor 35 Activation by Pyrroloquinoline Quinone Reviewed International journal

    Harumi Ando, Akari Kanamori, Mayuka Wakabayashi, Fumie Nakashima, Haruka Sei, Hiroyuki Hattori, Masaki Kita, Asuka Inoue, Ayana Murakami, Mitsugu Akagawa, Koji Uchida, Takahiro Shibata

    Biochemistry   Vol. 64 ( 24 ) page： 4758 - 4767   2025.11

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    Publishing type：Research paper (scientific journal)   Publisher：American Chemical Society (ACS)  

    DOI： 10.1021/acs.biochem.5c00566

  6. Dietary anthocyanidin pelargonidin activates G protein‐coupled receptor 35 Reviewed International journal Open Access

    Fumie Nakashima, Sayako Shimomura, Mayuka Wakabayashi, Wei Qi Loh, Harumi Ando, Haruka Sei, Hiroyuki Hattori, Didik Huswo Utomo, Masaki Kita, Asuka Inoue, Koji Uchida, Takahiro Shibata

    The FEBS Journal   Vol. 293 ( 5 ) page： 1386 - 1399   2025.10

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    Publishing type：Research paper (scientific journal)   Publisher：Wiley  

    G protein‐coupled receptors (GPCRs) are the largest superfamily of cell surface receptors. They regulate critical physiological events and serve as potential therapeutic targets. G protein‐coupled receptor 35 (GPR35), a class A rhodopsin‐like GPCR expressed in various tissues, including adipose tissue and the gastrointestinal tract, has roles in diverse functions, including antioxidant, anticarcinogenic, and anti‐inflammatory effects. Although many endogenous and synthetic GPR35 agonists have been identified, the understanding of food‐derived agonists is limited. In this study, we discovered pelargonidin as a newly identified food‐derived GPR35 agonist through a systematic screening approach. We evaluated 28 dietary phytochemicals using a transforming growth factor α (TGFα) shedding assay to evaluate GPR35 activation, and found that cyanidin, a common 3‐hydroxyanthocyanidin present in various red fruits and vegetables, induced GPR35 activation. Among a series of 3‐hydroxyanthocyanidins tested, pelargonidin, characterized by its monohydroxylated B‐ring, exhibited the most potent agonistic activity. Mutational studies demonstrated that the hydrogen bond between the 3‐hydroxy group in the C‐ring of pelargonidin and Asn169, as well as the hydrophobic interaction between the A‐ring of pelargonidin and Phe163, is crucial for GPR35 activation. Furthermore, pelargonidin inhibited the production of interleukin‐8, a pro‐inflammatory cytokine, by activating endogenous GPR35 in Caco‐2 cells. These findings suggest that GPR35 may serve as a potential receptor for dietary anthocyanidins, such as pelargonidin, and provide new insights into the molecular mechanisms underlying the potential chemopreventive effects of anthocyanidins.

    DOI： 10.1111/febs.70311

    Open Access

    Other Link： https://febs.onlinelibrary.wiley.com/doi/full-xml/10.1111/febs.70311

  7. Isocitrate dehydrogenase 1 upregulation in urinary extracellular vesicles from proximal tubules of type 2 diabetic rats Reviewed International journal Open Access

    Haruka Sei, Naoya Hirade, Kohei Kamiya, Fumie Nakashima, Jun Yoshitake, Keiko Kano, Emi Mishiro‐Sato, Ryosuke Kikuchi, Koji Uchida, Takahiro Shibata

    The FASEB Journal   Vol. 38 ( 10 ) page： e23688   2024.5

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    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Wiley  

    Abstract

    Diabetic nephropathy (DN) is a major cause of chronic kidney disease. Microalbuminuria is currently the most common non‐invasive biomarker for the early diagnosis of DN. However, renal structural damage may have advanced when albuminuria is detected. In this study, we sought biomarkers for early DN diagnosis through proteomic analysis of urinary extracellular vesicles (uEVs) from type 2 diabetic model rats and normal controls. Isocitrate dehydrogenase 1 (IDH1) was significantly increased in uEVs from diabetic model rats at the early stage despite minimal differences in albuminuria between the groups. Calorie restriction significantly suppressed the increase in IDH1 in uEVs and 24‐hour urinary albumin excretion, suggesting that the increase in IDH1 in uEVs was associated with the progression of DN. Additionally, we investigated the origin of IDH1‐containing uEVs based on their surface sugar chains. Lectin affinity enrichment and immunohistochemical staining showed that IDH1‐containing uEVs were derived from proximal tubules. These findings suggest that the increase in IDH1 in uEVs reflects pathophysiological alterations in the proximal tubules and that IDH1 in uEVs may serve as a potential biomarker of DN in the proximal tubules.

    DOI： 10.1096/fj.202400371r

    Open Access

  8. Rapid Isolation of Extracellular Vesicles Using a Hydrophilic Porous Silica Gel-Based Size-Exclusion Chromatography Column Reviewed International journal Open Access

    Jun Yoshitake, Mayuko Azami, Haruka Sei, Daisuke Onoshima, Kumiko Takahashi, Akiyoshi Hirayama, Koji Uchida, Yoshinobu Baba, Takahiro Shibata

    Analytical Chemistry   Vol. 94 ( 40 ) page： 13676 - 13681   2022.9

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    Publishing type：Research paper (scientific journal)   Publisher：American Chemical Society (ACS)  

    DOI： 10.1021/acs.analchem.2c01053

    Open Access

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Presentations 16

  1. 近位尿細管上皮細胞におけるフェロトーシス耐性機構の解明

    清日香, 中島史恵, 髙原照直, 柴田貴広

    日本酸化ストレス学会東海支部第13回学術集会  2025.2 

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    Presentation type：Poster presentation  

  2. Lectin-based enrichment of extracellular vesicles derived from rat renal proximal tubular cells

    Haruka Sei, Fumie Nakashima, Takahiro Shibata

    International Society for Extracellular Vesicles 2025 Annual Meeting  2025.4 

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    Presentation type：Poster presentation  

  3. Isocitrate dehydrogenase 1 is increased in urinary extracellular vesicles from type 2 diabetic model rats

    Haruka Sei, Naoya Hirade, Fumie Nakashima, Ryosuke Kikuchi, Tadashi Matsushita, Koji Uchida, Takahiro Shibata

    International Society for Extracellular Vesicles 2024 Annual Meeting  2024.5 

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    Presentation type：Poster presentation  

  4. Isocitrate dehydrogenase 1 is increased in urinary extracellular vesicles from type 2 diabetic model rats

    Haruka Sei, Naoya Hirade, Fumie Nakashima, Ryosuke Kikuchi, Tadashi Matsushita, Koji Uchida, Takahiro Shibata

    Glyco-Core Symposium 2024  2024.7 

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    Presentation type：Poster presentation  

  5. Analysis of ferroptosis-resistant renal proximal tubular cells

    Haruka Sei, Fumie Nakashima, Terunao Takahara, Takahiro Shibata

    Glyco-Core Symposium 2025  2025.9 

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    Presentation type：Poster presentation  

  6. 2型糖尿病モデルラット尿由来EV中タンパク質の解析

    清日香, 中島史恵, 菊地良介, 柴田貴広

    第88回日本生化学会中部支部例会・シンポジウム  2024.5 

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    Presentation type：Poster presentation  

  7. 2型糖尿病モデルラットにおける尿中細胞外小胞の解析 Invited

    清日香, 平出直哉, 中島史恵, 加納圭子, 三城-佐藤恵美, 菊地良介, 内田浩二, 柴田貴広

    第26回日本質量分析学会中部談話会  2024.3 

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    Presentation type：Oral presentation (invited, special)  

  8. Lens culinaris lectin selectively enriches rat proximal tubule-derived extracellular vesicles

    Haruka Sei, Fumie Nakashima, Mishiro-Sato Emi, Takahiro Shibata

    International Society for Extracellular Vesicles 2026  2026.4 

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    Presentation type：Poster presentation  

  9. フェロトーシス耐性細胞の樹立とその分子機構の解析

    清日香, 中島史恵, 髙原照直, 柴田貴広

    日本農芸化学会2025年度大会  2025.3 

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    Presentation type：Poster presentation  

  10. フェロトーシス耐性腎臓近位尿細管上皮細胞の耐性獲得原因の探索

    清日香, 中島史恵, 内田浩二, 柴田貴広

    第97回日本生化学会大会  2024.11 

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    Presentation type：Poster presentation  

  11. ラット尿に含まれる近位尿細管由来細胞外小胞の選択的濃縮法の確立

    清日香, 中島史恵, 三城-佐藤恵美, 内田浩二, 柴田貴広

    第12回日本細胞外小胞学会学術集会  2025.10 

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    Presentation type：Poster presentation  

  12. 尿中細胞外小胞を用いた糖尿病性腎症早期段階の解析

    清日香, 平出直哉, 中島史恵, 服部浩之, 菊地良介, 松下正, 内田浩二, 柴田貴広

    日本農芸化学会中部支部第193回例会  2022.10 

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    Presentation type：Oral presentation (general)  

  13. 糖尿病性腎症マーカーを含む尿中細胞外小胞由来部位の解析

    清日香, 平出直哉, 中島史恵, 菊地良介, 松下正, 内田浩二, 柴田貴広

    第10回日本細胞外小胞学会学術集会  2023.10 

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    Presentation type：Poster presentation  

  14. 糖尿病性腎症マーカータンパク質を含む尿中細胞外小胞の由来部位の解析

    清日香, 平出直哉, 中島史恵, 菊地良介, 松下正, 内田浩二, 柴田貴広

    第96回日本生化学会大会  2023.10 

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    Presentation type：Poster presentation  

  15. 糖尿病性腎症早期マーカーの開発を目指した尿由来細胞外小胞の解析

    清日香, 平出直哉, 中島史恵, 服部浩之, 菊地良介, 松下正, 内田浩二, 柴田貴広

    第9回日本細胞外小胞学会学術集会  2022.10 

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    Presentation type：Poster presentation  

  16. 細胞外小胞に含まれる糖尿病性腎症早期マーカーの探索

    清日香, 平出直哉, 中島史恵, 服部浩之, 菊地良介, 松下正, 内田浩二, 柴田貴広

    第95回日本生化学会大会  2022.11 

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    Presentation type：Oral presentation (general)  

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KAKENHI (Grants-in-Aid for Scientific Research) 4

  1. ACSL4の空間的制御に着目したフェロトーシス制御機構の解明

    2026.8 - 2028.3

    「次世代研究者」×「ＵＲＡ」基礎研究促進プログラム 

    清日香

  2. フェロトーシスにおけるACSL4の空間的制御機構の解明

    Grant number：26K24809  2026.7 - 2028.3

    日本学術振興会  科学研究費助成事業  研究活動スタート支援

    清 日香

  3. 細胞外小胞を基軸とした糖尿病性腎症超早期段階の解析

    Grant number：24KJ1250  2024.4 - 2026.3

    日本学術振興会  科学研究費助成事業  特別研究員奨励費

    清 日香

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    これまでに２型糖尿病モデルラットの尿由来EVにおいてIDH1が有意に増加することをプロテオミクスおよびウェスタンブロッティングによって見出している。腎臓の免疫組織染色を行った結果、IDH1はラット腎臓の近位尿細管と集合管に多く発現していることが示唆された。しかしながら、EV内タンパク質量はEVの分泌量やEVへの積み込み量も関与するため、腎臓での発現量が尿由来EV内のタンパク質プロファイルと完全に一致するとは言い難い。そこで、糖結合タンパク質であるレクチンを用いて尿由来EVのプルダウンを試みた。ラット尿由来EVをレクチンアレイに供した結果、EVは様々な種類の糖を表面に有していた。複数のレクチンを使用してスクリーニングを行った結果、LCAレクチンを使用すると近位尿細管由来EVと集合管由来EVを分画できることを発見した。この手法を用いることによって、尿由来EV中IDH1は主に近位尿細管由来EVに内包されていることが明らかになった。この結果より、糖尿病性腎症の超早期段階ではまず近位尿細管に変化が起きている可能性があり、近位尿細管の重要性が示唆された。
    糖尿病性腎症の進展に深く関連している細胞死としてフェロトーシスが知られている。この制御機構を理解することは糖尿病性腎症の機序解明において重要である。そこで、近位尿細管上皮細胞株に対してフェロトーシス誘導剤を複数回暴露させ、フェロトーシスに耐性のある細胞株であるクローンA、およびクローンBを樹立した。その耐性獲得機構の探索を行ったところ、いずれのクローンにもACSL4に変異が入っていることが示唆された。現在詳細なフェロトーシス耐性獲得の分子メカニズムの解析を進めている。

  4. 生活習慣病に関連する細胞外小胞の解明

    2023.4 - 2024.3

    東海国立大学機構  融合フロンティア次世代研究事業 

    清 日香

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    Authorship：Principal investigator 

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