2024/03/25 更新

写真a

ウサミ アツシ
宇佐見 享嗣
USAMI Atsushi
所属
高等研究院 特任助教
トランスフォーマティブ生命分子研究所 特任助教
職名
特任助教
外部リンク

学位 1

  1. 博士(工学) ( 2020年3月   名古屋大学 ) 

研究キーワード 7

  1. 香料

  2. 生物活性物質

  3. 生物変換

  4. 生体触媒

  5. 昆虫

  6. 微生物

  7. ナノカーボン

研究分野 5

  1. ナノテク・材料 / ケミカルバイオロジー  / 生物変換、ナノカーボン、昆虫、微生物、ライフサイエンス

  2. ライフサイエンス / 生物有機化学

  3. ライフサイエンス / 分子生物学

  4. ものづくり技術(機械・電気電子・化学工学) / バイオ機能応用、バイオプロセス工学

  5. ナノテク・材料 / ナノバイオサイエンス

現在の研究課題とSDGs 1

  1. 生体触媒による機能性ナノカーボン材料の創製

経歴 5

  1. 名古屋大学   高等研究院   YLC特任助教

    2023年4月 - 現在

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  2. 名古屋大学   大学院理学研究科   研究員

    2022年2月 - 2023年3月

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  3. 名古屋大学   物質科学国際研究センター   研究員

    2021年4月 - 2022年1月

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  4. 名古屋大学   大学院理学研究科   研究員

    2020年4月 - 2021年3月

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  5. 独立行政法人日本学術振興会 (名古屋大学) DC2

    2018年4月 - 2020年3月

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所属学協会 4

  1. 有機合成化学協会

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  2. 日本農芸化学会

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  3. 日本応用動物昆虫学会

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  4. 日本化学会

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受賞 5

  1. 優秀賞

    2023年12月   公益財団法人 中部科学技術センター  

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  2. コニカミノルタ研究企画賞

    2023年12月   有機合成化学協会  

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  3. 名古屋大学鏡友会 博士学術賞

    2019年10月   名古屋大学  

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  4. FEMS CONGRESS ATTENDANCE GRANT, the 8th FEMS Congress of European Microbiologists

    2019年7月   Federation of European Microbiological Societies  

    宇佐見享嗣

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  5. 第10回ベストプレゼンテーション賞

    2012年10月   第56 回香料•テルペンおよび精油化学に関する討論会  

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論文 25

  1. Gas-phase bioproduction of a high-value-added monoterpenoid (<i>E</i>)-geranic acid by metabolically engineered <i>Acinetobacter</i> sp. Tol 5 査読有り

    Usami, A; Ishikawa, M; Hori, K

    GREEN CHEMISTRY   22 巻 ( 4 ) 頁: 1258 - 1268   2020年2月

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    担当区分:筆頭著者   掲載種別:研究論文(学術雑誌)   出版者・発行元:Green Chemistry  

    Gas-phase bioproduction, in which immobilized biocatalysts are employed and chemical reactions are performed in a gas phase, has attracted researchers' attention as a green process. However, there is difficulty in the employment of whole cell catalysts for gas-phase bioproduction due to the lack of a suitable cell immobilization method. Acinetobacter sp. Tol 5 is a unique bacterium, which is remarkably sticky and can be easily immobilized onto various material surfaces through the adhesive bacterionanofiber protein AtaA. In this study, we demonstrate the gas-phase bioproduction of (E)-geranic acid (GA), a high-value-added monoterpenoid, from geraniol using immobilized Tol 5 transformant cells, into which a gene involved in a (E)-GA synthetic pathway was introduced. Time course analysis of the liquid-phase bioproduction of (E)-GA revealed the inherent metabolism of Tol 5 involved in the degradation of (E)-GA. By disrupting the fadD4-ortholog gene, which encodes a key enzyme of the (E)-GA degradation, we successfully generated a (E)-GA-accumulating strain, Tol 5 ΔfadD4 (pGeoA). The immobilized cells of this mutant strain on a polyurethane support enabled the production of (E)-GA with a passive supply of gaseous geraniol in a batch gas-phase reaction. A major fraction of the (E)-GA, which was produced, was adsorbed onto the polyurethane support but easily extracted into ethanol, a safe solvent without environmental impact. This is the first example of gas-phase bioproduction of a complex and high-value-added compound. Tol 5 is a highly promising platform for gas-phase bioproduction.

    DOI: 10.1039/c9gc03478a

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  2. Heterologous expression of geraniol dehydrogenase for identifying the metabolic pathways involved in the biotransformation of citral by <i>Acinetobacter</i> sp Tol 5 査読有り

    Usami, A; Ishikawa, M; Hori, K

    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY   82 巻 ( 11 ) 頁: 2012 - 2020   2018年11月

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    担当区分:筆頭著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Bioscience, Biotechnology and Biochemistry  

    The biotransformation of citral, an industrially important monoterpenoid, has been extensively studied using many microbial biocatalysts. However, the metabolic pathways involved in its biotransformation are still unclear, because citral is a mixture of the trans-isomer geranial and the cis-isomer neral. Here, we applied the heterologous expression of geoA, a gene encoding geraniol dehydrogenase that specifically converts geraniol to geranial and nerol to neral, to identify the metabolic pathways involved in the biotransformation of citral. Acinetobacter sp. Tol 5 was employed in order to demonstrate the utility of this methodology. Tol 5 transformed citral to (1R,3R,4R)-1-methyl-4-(1-methylethenyl)-1,3-cyclohexanediol and geranic acid. Biotransformation of citral precursors (geraniol and nerol) by Tol 5 transformant cells expressing geoA revealed that these compounds were transformed specifically from geranial. Our methodology is expected to facilitate a better understanding of the metabolic pathways involved in the biotransformation of substrates that are unstable and include geometric isomers.

    DOI: 10.1080/09168451.2018.1501263

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  3. Chemical composition, aroma evaluation, and inhibitory activity towards acetylcholinesterase of essential oils from Gynura bicolor DC 査読有り

    Mitsuo Miyazawa, Hiroshi Nakahashi, Atsushi Usami, Naoki Matsuda

    Journal of Natural Medicines   70 巻 ( 2 ) 頁: 282 - 289   2016年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Springer Tokyo  

    The compositions of the essential oils obtained from leaves and stems of Gynura bicolor DC. were analyzed by GC-MS. One hundred eight components of these oils were identified. (E)-β-caryophyllene (31.42 %), α-pinene (17.11 %), and bicyclogermacrene (8.09 %) were found to be the main components of the leaf oil, while α-pinene (61.42 %), β-pinene (14.39 %), and myrcene (5.10 %) were the major constituents of the stem oil. We found 73 previously unidentified components in these oils from G. bicolor. The oils were also subjected to odor evaluation. Eleven and 12 aroma-active compounds were detected in the leaf and stem oils, respectively. The abilities of these oils to inhibit acetylcholinesterase (AChE) activity were determined. The sesquiterpenoids in the oils were found to inhibit AChE activity more strongly than the monoterpenoids in the oils did. It was suggested that the three main components in each essential oil act synergistically against AChE activity. These results show that the essential oils obtained from G. bicolor are a good dietary source of AChE activity inhibition.

    DOI: 10.1007/s11418-015-0961-1

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  4. Sexual differences in chemical composition and aroma-active compounds of essential oil from flower buds of eurya japonica 査読有り

    Mitsuo Miyazawa, Atsushi Usami, Takio Tanaka, Kaoru Tsuji, Manami Takehara, Yuki Hori

    Journal of Oleo Science   65 巻 ( 4 ) 頁: 357 - 363   2016年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Japan Oil Chemists Society  

    This study was conducted to determine the composition of essential oil from buds of male and female Eurya japonica flowers and to determine the aroma-active compounds of this plant by gas chromatography-mass spectrometry (GC-MS), sensory evaluation, and odor activity values (OAV). The oils contained eighty-five compounds. We identified for the first time forty-four compounds in E. japonica. Through sensory evaluation, nineteen aroma-active compounds were identified by gas chromatographyolfactometry (GC-O). Because the chemical composition can affect the interaction between plants and herbivorous insects, our results suggest that essential oils from male and female flower buds of E. japonica differently affect herbivores. Sexual differences in essential oils deserve further investigations in this plantinsect system.

    DOI: 10.5650/jos.ess15295

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  5. Chemical composition and characteristic odor compounds in essential oil from alismatis rhizoma (Tubers of alisma orientale) 査読有り

    Mitsuo Miyazawa, Seiji Yoshinaga, Yusei Kashima, Hiroshi Nakahashi, Nobuyuki Hara, Hiroki Nakagawa, Atsushi Usami

    Journal of Oleo Science   65 巻 ( 1 ) 頁: 91 - 97   2016年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Japan Oil Chemists Society  

    Chemical composition and potent odorants that contribute to the characteristic odor of essential oil from Alismatis Rhizoma (tubers of Alisma orientale) were investigated by gas chromatography-mass spectrometry (GC-MS), GC-olfactometry (GC-O), aroma extract dilution analysis (AEDA) and relative flavor activity (RFA) methods. Fifty components, representing 94.5% of the total oil, were identified. In this study, we newly identified thirty-nine compounds in the oil from tubers of A. orientale. The major constituents of the essential oil were khusinol (36.2%), δ-elemene (12.4%), germacron (4.1%), alismol (3.8%), β-elemene (3.1%), and α-bisabolol (1.9%). Through sensory analysis, sixteen aroma-active compounds were detected and the key contributing aroma-active compounds were δ-elemene (woody, flavor dilution (FD)-factor = 4, RFA = 0.3) β-elemene (spicy, FD = 5, RFA = 0.7), spathulenol (green, FD = 5, RFA = 1.0), γ-eudesmol (woody, FD = 6, RFA = 1.5), and γ-cadinol (woody, FD = 5, RFA = 1.0). These compounds are thought to contribute to the odor from tubers of A. orientale. These results imply that the essential oil from the tubers of A. orientale deserve further investigations in the phytochemical and medicinal fields.

    DOI: 10.5650/jos.ess15176

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  6. Chemical composition and aroma evaluation of essential oils from skunk cabbage (Symplocarpus foetidus) 査読有り

    Mitsuo Miyazawa, Hiroshi Nakahashi, Yusei Kashima, Ryota Motooka, Nobuyuki Hara, Hiroki Nakagawa, Takashi Yoshii, Atsushi Usami, Shinsuke Marumoto

    Journal of Oleo Science   64 巻 ( 12 ) 頁: 1329 - 1336   2015年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Japan Oil Chemists Society  

    Two sample preparation methods, namely hydrodistillation (HD) and solvent-assisted flavor evaporation (SAFE), have been used to investigate the essential oils of the aerial parts (leaves and stems) of Symplocarpus foetidus, a plant with a characteristic odor, by gas chromatography mass spectrometry (GCMS). Characteristic aroma-active compounds in the oils were detected by GC-Olfactometry (GC-O) and aroma extract dilution analysis (AEDA). From the HD method, the main compounds in the oil were found to be p-vinyl-guaiacol (15.5%), 2-pentyl-furan (13.4%), and (Z)-ligustilide (9.5%). From the SAFE method, the main compounds were 2-butoxy-ethanol (49.6%), ethyl-pentanoate (4.5%), and mesitylene (4.0%). In HD oil, the most intense aroma-active compounds were 2-pentyl-furan (flavor dilution factor (FD) = 32, odor activity value (OAV) = 57), p-vinyl-guaiacol (FD = 16, OAV = 41), and dimethyl disulfide (FD = 16, OAV = 41). In SAFE oil, the main aroma-active compounds were 2-butoxy ethanol (FD = 32, OAV = 16), and 2-methoxy thiazole (FD = 32, OAV = 25).

    DOI: 10.5650/jos.ess15161

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  7. Metabolism of (-)-cis- and (-)-trans-rose oxide by cytochrome P450 enzymes in human liver microsomes 査読有り

    Hiroshi Nakahashi, Yuuki Yamamura, Atsushi Usami, Pramoch Rangsunvigit, Pomthong Malakul, Mitsuo Miyazawa

    Biopharmaceutics and Drug Disposition   36 巻 ( 9 ) 頁: 565 - 574   2015年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:John Wiley and Sons Ltd  

    The in vitro metabolism of (-)-cis- and (-)-trans-rose oxide was investigated using human liver microsomes and recombinant cytochrome P450 (P450 or CYP) enzymes for the first time. Both isomers of rose oxide were incubated with human liver microsomes, and the formation of the respective 9-oxidized metabolite were determined using gas chromatography-mass spectrometry (GC-MS). Of 11 different recombinant human P450 enzymes used, CYP2B6 and CYP2C19 were the primary enzymes catalysing the metabolism of (-)-cis- and (-)-trans-rose oxide. CYP1A2 also efficiently oxidized (-)-cis-rose oxide at the 9-position but not (-)-trans-rose oxide. α-Naphthoflavone (a selective CYP1A2 inhibitor), thioTEPA (a CYP2B6 inhibitor) and anti-CYP2B6 antibody inhibited (-)-cis-rose oxide 9-hydroxylation catalysed by human liver microsomes. On the other hand, the metabolism of (-)-trans-rose oxide was suppressed by thioTEPA and anti-CYP2B6 at a significant level in human liver microsomes. However, omeprazole (a CYP2C19 inhibitor) had no significant effects on the metabolism of both isomers of rose oxide. Using microsomal preparations from nine different human liver samples, (-)-9-hydroxy-cis- and (-)-9-hydroxy-trans-rose oxide formations correlated with (S)-mephenytoin N-demethylase activity (CYP2B6 marker activity). These results suggest that CYP2B6 plays important roles in the metabolism of (-)-cis- and (-)-trans-rose oxide in human liver microsomes. Copyright © 2015 John Wiley &amp
    Sons, Ltd.

    DOI: 10.1002/bdd.1965

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  8. Chemical Composition and Character Impact Odorants in Volatile Oils from Edible Mushrooms 査読有り

    Atsushi Usami, Ryota Motooka, Hiroshi Nakahashi, Shinsuke Marumoto, Mitsuo Miyazawa

    Chemistry and Biodiversity   12 巻 ( 11 ) 頁: 1734 - 1745   2015年11月

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    担当区分:筆頭著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Wiley-VCH Verlag  

    The aim of this study was to investigate the chemical composition and the odor-active components of volatile oils from three edible mushrooms, Pleurotus ostreatus, Pleurotus eryngii, and Pleurotus abalonus, which are well-known edible mushrooms. The volatile components in these oils were extracted by hydrodistillation and identified by GC/MS, GC-olfactometry (GC-O), and aroma extract dilution analysis (AEDA). The oils contained 40, 20, and 53 components, representing 83.4, 86.0, and 90.8% of the total oils in P. ostreatus, P. eryngii, and P. abalonus, respectively. Odor evaluation of the volatile oils from the three edible mushrooms was also carried out using GC-O, AEDA, and odor activity values, by which 13, eight, and ten aroma-active components were identified in P. ostreatus, P. eryngii, and P. abalonus, respectively. The most aroma-active compounds were C8-aliphatic compounds (oct-1-en-3-ol, octan-3-one, and octanal) and/or C9-aliphatic aldehydes (nonanal and (2E)-non-2-enal).

    DOI: 10.1002/cbdv.201400395

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  9. Evaluation of the key odorants in volatile oils from tubers of Apios americana Medikus 査読有り

    Hiroshi Nakahashi, Yuka Nishino, Hiroki Nakagawa, Nobuyuki Hara, Atsushi Usami, Mitsuo Miyazawa

    Journal of Oleo Science   64 巻 ( 11 ) 頁: 1235 - 1242   2015年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Japan Oil Chemists Society  

    This study was investigated the chemical composition of volatile oils and aroma evaluation from the tubers of Apios americana Medikus. Theses volatile oils were obtained by the hydrodistillation (HD) and the solvent-assisted flavor evaporation (SAFE) methods. These oils were analyzed by Gas chromatography (GC), GC-mass spectrometry (GC-MS), GC-olfactometry (GC-O), aroma extract dilution analysis (AEDA) and odor activity values (OAV) for the first time. The major compounds in the HD oil were palmitic acid (36.5%), linoleic acid (10.5%) and nonadecanol (5.7%). Meanwhile, in the SAFE oil, the major compounds were 4-hydroxy-4-methyl-2-pentanone (34.2%), hexanal (11.0%) and hexanol (7.9%). Through aroma evaluation, 20 (HD) and 14 (SAFE) aroma-active compounds were identified by GC-O. As a result, the most intense aroma-active compounds in both extraction methods were 1-octen-3-ol and hexanal, both of which showed high odor activity values (OAV).

    DOI: 10.5650/jos.ess15132

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  10. Chemical compositions and aroma evaluation of volatile oil from the industrial cultivation medium of Enterococcus faecalis 査読有り

    Toshirou Ono, Atsushi Usami, Satoshi Nakaya, Keisuke Maeba, Yasunori Yonejima, Masanori Toyoda, Atsushi Ikeda, Mitsuo Miyazawa

    Journal of Oleo Science   64 巻 ( 10 ) 頁: 1125 - 1133   2015年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Japan Oil Chemists Society  

    Enterococcus faecalis is one of the major lactic acid bacterium (LAB) species colonizing the intestines of animals and humans. The characteristic odor of the volatile oils obtained from both the liquid medium after incubation (MAI) and liquid medium before incubation (MBI) in the cultivation process of E. faecalis was investigated to determine the utility of the liquid medium. In total, fifty-six and thirty-two compounds were detected in the volatile oils from the MAI (MAI oil) and MBI (MBI oil), respectively. The principle components of MAI oil were 2,5-dimethylpyrazine (19.3%), phenylacetaldehyde (19.3%), and phenylethyl alcohol (9.3%). The aroma extract dilution analysis (AEDA) method was performed using gas chromatography-olfactometry (GC-O). The total number of aroma-active compounds identified in the volatile oil from MBI and MAI was thirteen compounds
    in particular, 5-methyl-2-furanmethanol, phenylacetaldehyde, and phenylethyl alcohol were the most primary aroma-active compounds in MAI oil. These results imply that the industrial cultivation medium after incubation of E. faecalis may be utilized as a source of volatile oils.

    DOI: 10.5650/jos.ess15098

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  11. Volatile compounds with characteristic odor of essential oil from Magnolia obovata leaves by hydrodistillation and solvent-assisted flavor evaporation 査読有り

    Mitsuo Miyazawa, Yoshimi Nakashima, Hiroshi Nakahashi, Nobuyuki Hara, Hiroki Nakagawa, Atsushi Usami, Warinthorn Chavasiri

    Journal of Oleo Science   64 巻 ( 9 ) 頁: 999 - 1007   2015年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Japan Oil Chemists Society  

    The present study focuses on the volatile compounds with characteristic odor of essential oil from the leaves of Magnolia obovata by hydrodistillation (HD) and solvent-assisted flavor evaporation (SAFE) method. Eighty-seven compounds, representing 98.0% of the total oil, were identified using HD. The major compounds of HD oil were (E)-β-caryophyllene (23.7%), a-humulene (11.6%), geraniol (9.1%), and borneol (7.0%). In SAFE oil, fifty-eight compounds, representing 99.7% of the total oil, were identified. The main compounds of SAFE oil were (E)-β-caryophyllene (48.9%), α-humulene (15.7%), and bicyclogermacrene (4.2%). In this study, we newly identified eighty-five compounds of the oils from M. obovata leaves. These oils were also subjected to aroma evaluation by gas chromatography-olfactometry (GC-O) and aroma extract dilution analysis (AEDA). As a result, twenty-four (HD) and twenty-five (SAFE) aroma-active compounds were detected. (E)-β-Caryophyllene, α-humulene, linalool, geraniol, 1,8-cineole, and bicyclogermacrene were found to impart the characteristic odor of M. obovata leaves. These results imply that the oils of M. obovata leaves must be investigated further to clarify their potential application in the food and pharmaceutical industries.

    DOI: 10.5650/jos.ess15114

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  12. Identification of aroma-active compounds in essential oil from Uncaria Hook by gas chromatography- mass spectrometry and gas chromatography-olfactometry 査読有り

    Megumi Iwasa, Satoshi Nakaya, Yusuke Maki, Shinsuke Marumoto, Atsushi Usami, Mitsuo Miyazawa

    Journal of Oleo Science   64 巻 ( 8 ) 頁: 825 - 833   2015年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Japan Oil Chemists Society  

    The chemical composition of essential oil extracted from Uncaria Hook (“Chotoko” in Japanese), the branch with curved hook of the herbal medicine Uncaria rhynchophylla has been investigated by GC and GC-MS analyses. Eighty-four compounds, representing 90.8% of the total content was identified in oil obtained from Uncaria Hook. The main components i were (E)-cinnamaldehyde (13.4%), α-copaene (8.0%), methyl eugenol (6.8%), d-cadinene (5.3%), and curcumene (3.6%). The important key aroma-active compounds in the oil were detected by gas chromatography-olfactometry (GC-O) and aroma extract dilution analysis (AEDA), using the flavor dilution (FD) factor to express the odor potency of each compounds. Furthermore, the odor activity value (OAV) has been used as a measure of the relative contribution of each compound to the aroma of the Uncaria Hook oil. The GC-O and AEDA results showed that α-copaene (FD = 4, OAV = 4376), (E)-linalool oxide (FD = 64, OAV = 9.1), and methyl eugenol (FD = 64, OAV = 29) contributed to the woody and spicy odor of Uncaria Hook oil, whereas furfural (FD = 8, OAV = 4808) contributed to its sweet odor. These results warrant further investigations of the application of essential oil from Uncaria Hook in the phytochemical and medicinal fields.

    DOI: 10.5650/jos.ess15048

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  13. Agroecosystem Development of Industrial Fermentation Waste - Suppressive Effects of Beer Yeast Cell Wall Extract on Plant Diseases 査読有り

    Malek Marian, Kiriko Ogawa, Yui Yoshikawa, Satoko Takasaki, Atsushi Usami, Masafumi Shimizu, Mitsuo Miyazawa, Mitsuro Hyakumachi

    Current Environmental Engineering   1 巻 ( 3 ) 頁: 207 - 211   2015年5月

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:Bentham Science Publishers Ltd.  

    DOI: 10.2174/221271780103150522163942

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  14. Characteristic chemical components and aromaactive compounds of the essential oils from ranunculus nipponicus var. Submersus used in japanese traditional food 査読有り

    Satoshi Nakaya, Atsushi Usami, Tomohito Yorimoto, Mitsuo Miyazawa

    Journal of Oleo Science   64 巻 ( 6 ) 頁: 595 - 601   2015年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Japan Oil Chemists Society  

    Ranunculus nipponicus var. submersus is an aquatic macrophyte, it is known as a wild edible plant in Japan for a long time. In this study, the essential oils from the fresh and dried aerial parts of R. nipponicus var. submersus were extracted by hydrodistillation and analyzed by gas chromatography (GC) and GC-mass spectrometry (GC-MS). Moreover, important aroma-active compounds were also detected in the oil using GC-olfactometry (GC-O) and aroma extract dilution analysis (AEDA). Thus, 98 compounds (accounting for 93.86%) of the oil were identified. The major compounds in fresh plant oil were phytol (41.94%), heptadecane (5.92%), and geranyl propionate (5.76%), while those of. Dried plant oil were β- ionone (23.54%), 2-hexenal (8.75%), and dihydrobovolide (4.81%). The fresh and dried oils had the greenfloral and citrus-floral odor, respectively. The GC-O and AEDA results show that phenylacetaldehyde (green, floral odor, FD-factor = 8) and β-ionone (violet-floral odor, FD-factor = 8) were the most characteristic odor compounds of the fresh oils. β-Cyclocitral (citrus odor, FD-factor = 64) and β-ionone (violet-floral odor, FD-factor = 64) were the most characteristic odor compounds of the dried oil. These compounds are thought to contribute to the flavor of R. nipponicus var. submersus.

    DOI: 10.5650/jos.ess14265

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  15. Agroecosystem development of industrial fermentation waste -characterization of aroma-active compounds from the cultivation medium of Lactobacillus brevis 査読有り

    Toshirou Ono, Atsushi Usami, Satoshi Nakaya, Hideto Shinpuku, Yasunori Yonejima, Atsushi Ikeda, Mitsuo Miyazawa

    Journal of Oleo Science   64 巻 ( 5 ) 頁: 585 - 594   2015年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Japan Oil Chemists Society  

    Volatile oils obtained from both the liquid medium after incubation (MAI) and liquid medium before incubation (MBI) during the cultivation process of Lactobacillus brevis were isolated by hydrodistillation (HD) and analyzed to determine the utility of the liquid waste. The composition of the volatile oils was analyzed by capillary gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS). In total, 55 and 36 compounds were detected in the volatile oils from MAI (MAI oil) and MBI (MBI oil), respectively. The principle components of MAI oil were N-containing compounds, including 2,3-dimethylpyrazine (16, 37.1 %), methylpyrazine (4, 17.1 %). The important aroma-active compounds in the oils were detected by GC-Olfactometry (GC-O), and their intensity of aroma were measured by aroma extract dilution analysis (AEDA). Expressly, pyrazine compounds were determined as key aroma components
    in particular, 2,5-dimethylpyrazine and 2,3-dimethylpyrazine were the most primary aroma-active compound in MAI oil. These results imply that the waste medium after incubation of L. brevis may be utilized as a source of volatile oils.

    DOI: 10.5650/jos.ess14257

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  16. Volatile Composition and Aroma Evaluation of Chrysanthemum Coronarium 査読有り

    Mitsuo Miyazawa, Hinako Otsuka, Satoshi Nakaya, Atsushi Usami

    Journal of Biochemistry and Molecular Biology Research   1 巻 ( 2 ) 頁: 46 - 53   2015年

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:ACT Publishing Group  

    DOI: 10.17554/j.issn.2313-7177.2015.01.11

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  17. Characteristic odor components of essential oils from Eurya Japonica 査読有り

    Ryota Motooka, Atsushi Usami, Hiroshi Nakahashi, Satoshi Koutari, Satoshi Nakaya, Ryoyu Shimizu, Kaoru Tsuji, Shinsuke Marumoto, Mitsuo Miyazawa

    Journal of Oleo Science   64 巻 ( 5 ) 頁: 577 - 584   2015年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Japan Oil Chemists Society  

    The chemical compositions of essential oils from the flower and aerial parts (i.e., leaf and branch) of Eurya japonica were determined and quantified using gas chromatography-mass spectrometry (GC-MS). A total of 87 and 50 compounds were detected in the oils from the flower and aerial parts, respectively. The main compounds of the flower oil were linalool (14.0%), (9Z)-tricosene (12.0%), and nonanal (7.4%). In the oil from the aerial parts, linalool (37.7%), a-terpineol (13.5%), and geraniol (9.6%) were detected. In the oils from the flower and aerial parts, 13 and 8 aroma-active compounds were identified by GC-olfactometry (GC-O) analysis, respectively. The key aroma-active compounds of the flower oil were heptanal [fatty, green, flavor dilution (FD) = 128, odor activity value (OAV) = 346], nonanal (sweet, citrus, FD = 128, OAV = 491), and eugenol (sweet, spicy, FD = 64, OAV = 62): in the oil from the aerial parts, the key aroma-active compounds were linalool (sweet, citrus, FD = 64, OAV = 95), (E)-b-damascenone (sweet, FD = 256, OAV = 4000), and (E)-b-ionone (floral, violet, FD = 128, OAV = 120). This study revealed that nonanal and eugenol impart the sweet, citrus, and spicy odor of the flower oil, while (E)-b-damascenone and (E)-b-ionone contribute the floral and sweet odor of the oil from the aerial parts.

    DOI: 10.5650/jos.ess14225

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  18. Highly selective biotransformation of (+)-(1S)- and (-)-(1R)-camphorquinone by Aspergillus wentii 査読有り

    Atsushi Usami, Ryota Motooka, Mitsuo Miyazawa

    Biocatalysis and Biotransformation   32 巻 ( 5-6 ) 頁: 285 - 289   2014年12月

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    担当区分:筆頭著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Informa Healthcare  

    To clarify the structures of biotransformation products and metabolic pathways, the biotransformation of monoterpenoids, (+)- and (-)-camphorquinone (1a and b), has been investigated using Aspergillus wentii as a biocatalyst. Compound 1a was converted to (-)-(2S)-exo-hydroxycamphor (2a), (-)-(2S)-endo-hydroxycamphor (3a), (-)-(3S)-exo-hydroxycamphor (4a), (-)-(3S)-endo-hydroxycamphor (5a), and (+)-camphoric acid (6a). Compound 1b was converted to (+)-(2R)-exo-hydroxycamphor (2b), (+)-(2R)-endo-hydroxycamphor (3b), (+)-(3R)-exo-hydroxycamphor (4b), (+)-(3R)-endo-hydroxycamphor (5b), and (-)-camphoric acid (6b). Compound 1a mainly produced 2a (65.0%) with stereoselectivity, whereas 1b afforded 3b (84.3%) with high stereoselectivity. These structures were confirmed by gas chromatography-mass spectrometry, infrared, 1H nuclear magnetic resonance (NMR), and 13C NMR spectral data. The products illustrate the marked ability of A. wentii for enzymatic oxidation and ketone reduction.

    DOI: 10.3109/10242422.2014.975215

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  19. Aroma evaluation of setonojigiku (Chrysanthemum japonense var. debile) by hydrodistillation and solvent-assisted flavour evaporation 査読有り

    Atsushi Usami, Hiroshi Nakahashi, Shinsuke Marumoto, Mitsuo Miyazawa

    Phytochemical Analysis   25 巻 ( 6 ) 頁: 561 - 566   2014年

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    担当区分:筆頭著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:John Wiley and Sons Ltd  

    Introduction - The Chrysanthemum genus consisting of about 200 species is mainly distributed over the Northern Hemisphere. Despite the pleasant odour of C. japonense var. debile (setonojigiku), no detailed analysis of the aroma-active compounds has been reported using sensory evaluation.
    Objectives - Using a hydrodistillation (HD) and a solvent-assisted flavour evaporation (SAFE) method to obtain the volatile oil from the leaf parts.
    Methods - To clarify odorants contributing to the characteristic aroma-active compounds, the aroma-extract dilution analysis (AEDA) method was performed through gas chromatography olfactometry (GC/O) analysis. In addition, the odour activity value (OAV) was calculated in order to determine the relative contribution of each compound to the aroma-active compounds.
    Results - A total of 42 components by HD oil were identified by GC-MS, whereas 34 components were identified in SAFE oil. Thirteen compounds were identified by GC/O analysis in HD and SAFE oils respectively.
    Conclusion - Each extraction method has its own advantages and disadvantages, and they are generally complementary to each other. On the basis of AEDA, OAV and sensory evaluations, [2.2.1] bicyclic monoterpenes (borneol, bornyl acetate and camphor) and β-caryophyllene are considered to be the main aroma-active compounds of both extraction methods.

    DOI: 10.1002/pca.2528

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  20. Comparison of agitake (Pleurotus eryngii var. ferulae) volatile components with characteristic odors extracted by hydrodistillation and solventassisted flavor evaporation 査読有り

    Atsushi Usami, Toshirou Ono, Yusei Kashima, Hiroshi Nakahashi, Shinsuke Marumoto, Sota Nosaka, Shogo Watanabe, Mitsuo Miyazawa

    Journal of Oleo Science   63 巻 ( 1 ) 頁: 83 - 92   2014年

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    担当区分:筆頭著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Japan Oil Chemists{\textquotesingle} Society  

    The chemical composition of volatile oil from agitake (Pleurotus eryngii var. ferulae) was established for the first time using gas chromatography (GC) and GC-mass spectrometry. Sixty-seven and 24 components were extracted by hydrodistillation (HD) using diethyl ether (DE) and dichloromethane (DM), respectively
    these components accounted for 80.3% and 91.8% of the total oil, respectively. Thirteen and 48 components of were extracted by the solvent-assisted flavor evaporation method (SAFE), using DE and DM, respectively, and identified
    these components accounted for 83.5% and 82.0% of the total oil, respectively. Methylsuccinimide and 2,3,7-trimethyl-2-octene were the most characteristic components by SAFE using DM. Odor evaluation of the volatile oil from agitake was also carried out using GC-olfactometry (GC-O), aroma extraction dilution analysis (AEDA), and the odor activity value (OAV). Sixteen, 8, 5 and 9 aromaactive components were identified using HD (DE and DM) and SAFE (DE and DM), respectively. The main aroma-active components extracted using HD and SAFE were 1-octen-3-ol (mushroom-like) and phenylacetaldehyde (floral), respectively. This study proved that HD and SAFE can be used as complementary extraction techniques for the complete characterization of volatile oil from agitake. © 2014 by Japan Oil Chemists' Society.

    DOI: 10.5650/jos.ess13043

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  21. Chemical composition, aroma evaluation, and oxygen radical absorbance capacity of volatile oil extracted from Brassica rapa cv. "yukina" used in Japanese traditional food 査読有り

    Atsushi Usami, Ryota Motooka, Ayumi Takagi, Hiroshi Nakahashi, Yoshiharu Okuno, Mitsuo Miyazawa

    Journal of Oleo Science   63 巻 ( 7 ) 頁: 723 - 730   2014年

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    担当区分:筆頭著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Japan Oil Chemists Society  

    The chemical composition of the volatile oil extracted from the aerial parts of Brassica rapa cv. f"yukina" was analyzed using GC-MS, GC-PFPD, and GC-O. A total of 50 compounds were identified. The most prominent constituents were (E)-1,5-heptadiene (40.27%), 3-methyl-3-butenenitrile (25.97%) and 3-phenylpropanenitrile (12.41%). With regard to aroma compounds, 12 compounds were identified by GC-O analysis. The main aroma-active compounds were dimethyl tetrasulfide (sulphury-cabbage, FD = 64), 3-phenylpropanenitrile (nutty, FD = 64), 3-methylindole (pungent, FD = 64), and methional (potato, FD = 32). The antioxidant activity of the aroma-active compounds of the oil was determined using an oxygen radical absorbance capacity (ORAC) assay using fluorescein as the fluorescent probe. The ORAC values were found to be 785 ± 67 trolox equivalents (μmol TE/g) for B. rapa cv. "yukina" oil. The results obtained showed that the volatile oil extracted from the aerial parts is a good dietary source of antioxidants. © 2014 by Japan Oil Chemists' Society.

    DOI: 10.5650/jos.ess14033

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  22. Chemical composition and aroma evaluation of volatile oils from edible mushrooms (Pleurotus salmoneostramineus and Pleurotus sajor-caju) 査読有り

    Atsushi Usami, Satoshi Nakaya, Hiroshi Nakahashi, Mitsuo Miyazawa

    Journal of Oleo Science   63 巻 ( 12 ) 頁: 1323 - 1332   2014年

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    担当区分:筆頭著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Japan Oil Chemists Society  

    This study is focused on the volatile oils from the fruiting bodies of Pleurotus salmoneostramineus (PS) and P. sajor-caju (PSC), which was extracted by hydrodistillation (HD) and solvent-assisted flavor evaporation (SAFE) methods. The oils are analyzed by gas chromatography-mass spectrometry (GC-MS), GC-olfactometry (GC-O), and aroma extract dilution analysis (AEDA). A total of 31, 31, 45, and 15 components were identified in PS (HD and SAFE) and PSC (HD and SAFE), representing about 80.3%, 92.2%, 88.9%, and 83.0% of the oils, respectively. Regarding the aroma-active components, 13, 12, 13, and 5 components were identified in PS (HD and SAFE) and PSC (HD and SAFE), respectively, by the GC-O analyses. The results of the sniffing test, odor activity value (OAV) and favor dilution (FD) factor indicate that 1-octen-3-ol and 3-octanone are the main aroma-active components of PS oils. On the other hands, methional and 1-octen-3-ol were estimated as the main aroma-active components of PSC oils.

    DOI: 10.5650/jos.ess14147

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  23. Characteristic odorants from bailingu oyster mushroom (Pleurotus eryngii var. tuoliensis) and summer oyster mushroom (Pleurotus cystidiosus) 査読有り

    Atsushi Usami, Ryota Motooka, Hiroshi Nakahashi, Yoshiharu Okuno, Mitsuo Miyazawa

    Journal of Oleo Science   63 巻 ( 7 ) 頁: 731 - 739   2014年

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    担当区分:筆頭著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Japan Oil Chemists Society  

    In this study, the characteristic odorants of the volatile oils from Pleurotus species (P. eryngii var. tuoliensis and P. cystidiosus) were extracted by hydrodistillation and analyzed by gas chromatography-mass spectrometry (GC-MS), gas chromatography-olfactometry (GC-O), and aroma extract dilution analysis (AEDA). A total of 52 and 54 components (P. eryngii var. tuoliensis and P. cystidiosus, respectively) were identified, representing about 98.8% and 85.1% of the volatile oils, respectively. The main components of the P. eryngii var. tuoliensis oil were palmitic acid (82, 38.0%), oleic acid (86, 25.0%) and linoleic acid (85, 9.7%). The main components of the P. cystidiosus oil, palmitic acid (82, 25.8%), indole (54, 9.1%) and myristic acid (77, 5.3%). Regarding the aroma components, 16 and 13 components were identified in the P. eryngii var. tuoliensis and P. cystidiosus oils respectively, by the GC-O analyses. The results of the sniffing test, odor activity value (OAV) and flavor dilution (FD) factor indicate that methional, 1-octen-3-ol and nonanal are the main aroma-active components of P. eryngii var. tuoliensis oil. On the other hands, dimethyl trisulfide and 1-octen-3-ol were estimated as the main aroma-active components of the P. cystidiosus oil. © 2014 by Japan Oil Chemists' Society.

    DOI: 10.5650/jos.ess14043

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  24. Characterization of aroma-active compounds in dry flower of Malva sylvestris L. by GC-MS-O analysis and OAV calculations 査読有り

    Atsushi Usami, Yusei Kashima, Shinsuke Marumoto, Mitsuo Miyazawa

    Journal of Oleo Science   62 巻 ( 8 ) 頁: 563 - 570   2013年8月

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    担当区分:筆頭著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Japan Oil Chemists{\textquotesingle} Society  

    In this study, the aroma-active compounds in the dried flower of Malva sylvestris L. were extracted by hydrodistillation and analyzed by gas chromatography-mass spectrometry (GC-MS), and gas chromatography-olfactometry (GC-O) and aroma extraction dilution analysis (AEDA). A light yellow oil with a sweet odor was obtained with a percentage yield of 0.039% (w/w), and 143 volatile compounds (89.86%) were identified by GC-MS. The main compounds were hexadecanoic acid (10.1%), pentacosane (4.8%) and 6,10,14-trimethyl-2-pentadecanone (4.1%). The essential oil consisted mainly of hydrocarbons (25.40%) followed by, alcohols (18.78%), acids (16.66%), ethers (5.01%) ketones (7.28%), esters(12.43%), aldehydes (2.30%) and others (2.00%). Of these compounds, 20 were determined by GC-O and AEDA, to be odor-active (FD (favor dilution) factor ≥ 1). (3-Damascenone (FD = 9, sweet), phenylacetaldehyde (FD = 8, foral, honey-like) and (E)-p-ocimene (FD = 8, spicy) were the most intense aroma-active compounds in M. sylvestris. In order to determine the relative contribution of each of the compounds to the aroma of M. sylvestris, odor activity values (OAVs) were used. p-Damascenone had the highest odor activity values (OAV) (50700), followed by (E)-p-ionone (15444) and decanal (3510). In particular, p-damascenone had a high FD factors, and therefore, this compound was considered to be the main aroma-active components of the essential oil. On the basis of AEDA, OAVs, and sensory evaluation results, p-damascenone is estimated to be the main aroma-active compound of the essential oil. © 2013 by Japan Oil Chemists' Society.

    DOI: 10.5650/jos.62.563

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  25. Comparison of volatile compounds with characteristic odor in flowers and leaves of Nojigiku (Chrysanthemum japonense) 査読有り

    Atsushi Usami, Toshirou Ono, Shinsuke Marumoto, Mitsuo Miyazawa

    Journal of Oleo Science   62 巻 ( 8 ) 頁: 631 - 636   2013年8月

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    担当区分:筆頭著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Japan Oil Chemists{\textquotesingle} Society  

    The aim of the present study was to investigate the essential oils isolated from fower and leaf in order to get insight into similarities and differences as to their aroma-active composition. The essential oil obtained from the two parts were analyzed by gas chromatography-mass spectrometry and gas chromatography olfactometry (GC-O). Flower and leaf oils, 38 and 36 constituents, representing 96.4 and 91.0% of the total oil composition, respectively, were identified. The main compounds in flower oil were camphor (47.64%), bornyl acetate (11.87%), and nojigiku alcohol (6.29%), whereas those in leaf oil were camphor (39.14%), nojigiku alcohol (10.76%) and y-muurolene (7.02%). 13 Aroma-active compounds were identifed by GC-O analysis in fower oil and 12 in leaf oil. The main aroma-active compounds in fower oil were camphor (camphor, FD (flavor dilution) = 7, OAV (odor active value) = 136913), bornyl acetate (camphor, FD = 6, OAV = 113711), and p-caryophyllene (spicy, FD = 5, OAV = 116480). In leaf oil, the main aroma-active compounds were camphor (camphor, FD = 7, OAV = 106784), nojigiku alcohol (camphor, FD = 5, OAV = not determined), and p-caryophyllene (spicy, FD = 6, OAV = 526267). © 2013 by Japan Oil Chemists' Society.

    DOI: 10.5650/jos.62.631

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▼全件表示

講演・口頭発表等 42

  1. 生物変換による酸素原子含有ナノベルトの合成

    河野英也, 宇佐見享嗣, 周戸大季, 天池一真, 八木, 亜樹子, 伊丹健一郎

    第32回基礎有機化学討論会  2022年9月21日 

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    開催年月日: 2022年9月

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  2. カチオン性ペリレンを用いた核酸輸送

    天池 一真, 加藤 江莉佳, 深津 美羽, Zetschok Dominik, 宇佐見 享嗣, 山田 早人, 伊丹 健一郎

    第16回バイオ関連化学シンポジウム  2022年9月11日 

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    開催年月日: 2022年9月

    会議種別:口頭発表(一般)  

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  3. Functionalization of cycloparaphenylene by biocatalysts

    Atsushi Usami, Kazuma Amaike, Katsutoshi Hori, Kenichiro Itami

    日本化学会 第102春季年会  2022年3月26日 

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    開催年月日: 2022年3月

    記述言語:英語   会議種別:口頭発表(一般)  

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  4. 新奇環状ジアリールエーテルの合成と性質

    加藤智紀, 河野英也, 宇佐見享嗣, 周戸大季, 八木亜樹子, 伊丹健一郎

    第54回構造有機化学若手の会 夏の学校  2023年8月2日 

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    会議種別:ポスター発表  

  5. 生体触媒としてハスモンヨトウを用いた(+)- および (-)-campheneの生物変換

    宇佐見享嗣, 神足悟史, 堀部功, 宮澤三雄

    第56回 香料•テルペンおよび精油化学に関する討論会  2012年10月27日 

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    会議種別:口頭発表(一般)  

  6. 双環性モノテルペン camphene を用いたハスモンヨトウによる変換反応

    宇佐見享嗣, 伊藤星都, 宮澤三雄

    日本油化学会フレッシュマンサミット OSAKA  2012年11月11日 

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    会議種別:口頭発表(一般)  

  7. 双環性モノテルペノイドCamphorquinoneを用いたハスモンヨトウによる生物変換

    宇佐見享嗣, 中谷聡志, 宮澤三雄

    日本油化学会第52回年会  2013年9月 

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    会議種別:ポスター発表  

  8. ハスモンヨトウによる環状モノテルペンエーテル Lime oxide T の代謝

    本岡良太, 宇佐見享嗣, 宮澤三雄

    日本油化学会第52回年会   2013年9月 

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    会議種別:ポスター発表  

  9. 生体触媒としてハスモンヨトウ (Spodoptera litura) を用いた Lime oxide T の生物変換

    本岡良太, 宇佐見享嗣, 宮澤三雄

    第57回 香料•テルペンおよび精油化学に関する討論会  2013年10月 

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    会議種別:口頭発表(一般)  

  10. 生体触媒としてハスモンヨトウを用いた Camphorquinone の生物変換

    宇佐見享嗣, 野阪創太, 宮澤三雄

    第57回 香料•テルペンおよび精油化学に関する討論会  2013年10月 

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    会議種別:口頭発表(一般)  

  11. Aspergillus wentii による (+)-(1S)-および(-)-(1R)-Camphorquinone の生物変換

    宇佐見享嗣, 本岡良太, 宮澤三雄

    日本農芸化学会2014年度大会  2014年3月 

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    会議種別:口頭発表(一般)  

  12. 生体触媒としてハスモンヨトウ (Spodoptera litura) を用いた (-)-myrtenal の生物変換

    本岡良太, 宇佐見享嗣, 宮澤三雄

    日本農芸化学会2014年度大会   2014年3月 

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    会議種別:口頭発表(一般)  

  13. Characteristic Odors and Food Security of Japanese Food, Agitake

    Atsushi Usami, Mitsuo Miyazawa

    105th AOCS Annual Meeting & Expo   2014年5月 

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    会議種別:ポスター発表  

  14. Production of new food flavor from myrtenol and myrtenal using Spodoptera litura as biocatalyst

    Ryota Motooka, Atsushi Usami, Mitsuo Miyazawa

    105th AOCS Annual Meeting & Expo   2014年5月 

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    会議種別:ポスター発表  

  15. Biotransformation of 3-octanone by white-rot fungus Pleurotus ostreatus as a biocatalyst

    Atsushi Usami, Mitsuo Miyazawa

    International Association of Plant Biotechnology Congress   2014年8月 

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    会議種別:ポスター発表  

  16. Chemical composition of volatile oils of mushrooms (Pleurotus species) and biotransformation of the mushroom odor component

    Atsushi Usami, Mitsuo Miyazawa

    1st Asian Conference on Oleo Science (ACOS)   2014年9月 

  17. 生体触媒として食用菌ヒラタケ (Pleurotus ostreatus) を用いた3-octanoneの生物変換

    宇佐見享嗣, 宮澤三雄

    第58回 香料•テルペンおよび精油化学に関する討論会  2014年9月 

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    会議種別:口頭発表(一般)  

  18. 生体触媒として微生物を用いた(+)- および (-)-α-pineneの生物変換

    本岡良太, 宇佐見享嗣, 宮澤三雄

    第58回 香料•テルペンおよび精油化学に関する討論会  2014年9月 

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    会議種別:口頭発表(一般)  

  19. モミタケの揮発性成分および香気特性

    北村祐貴, 宇佐見享嗣, 宮澤三雄

    第58回 香料•テルペンおよび精油化学に関する討論会  2014年9月 

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    会議種別:口頭発表(一般)  

  20. 座禅草(Symplocarpus foetidus)の揮発性成分および香気特性

    吉井崇, 宇佐見享嗣, 宮澤三雄

    第58回 香料•テルペンおよび精油化学に関する討論会  2014年9月 

     詳細を見る

    会議種別:口頭発表(一般)  

  21. Volatile compounds with characteristic odor of essential oil from Magnoia obovata leaves by hydrodistillation and solvent-assisted flavor evaporation

    Nobuyuki Hara, Yoshimi Nakashima, Hiroshi Nakahashi, Hiroki Nakagawa, Atsushi Usami, Warinthorn Chavasiri, Mitsuo Miyazawa

    International conference on biological science   2015年8月 

     詳細を見る

    会議種別:ポスター発表  

  22. Acinetobacter sp. Tol 5 による citral の位置立体選択的生物変換と自然酸化

    宇佐見 享嗣, 石川 聖人, 堀 克敏

    第11回 バイオ関連化学シンポジウム  2017年9月 

     詳細を見る

    会議種別:ポスター発表  

  23. Acinetobacter sp. Tol 5 を用いた citral の生体内変換と代謝経路の特定

    宇佐見 享嗣, 石川 聖人, 堀 克敏

    日本農芸化学会2018年度大会  2018年3月 

     詳細を見る

    会議種別:口頭発表(一般)  

  24. Geraniol dehydrogenase の異種発現による Acinetobacter sp. Tol 5 の citral 生物変換に関わる代謝経路の同定

    宇佐見 享嗣, 石川 聖人, 堀 克敏

    日本農芸化学会2019年度大会  2019年3月 

     詳細を見る

    会議種別:口頭発表(一般)  

  25. Green bioproduction of monoterpenoid (E)-geranic acid from geraniol by genetically engineered Acinetobacter sp. Tol 5 in gas phase

    Atsushi Usami, Masahito Ishikawa, Katsutosi Hori

    8th Congress of European Microbiologists, FEMS  2019年7月 

     詳細を見る

    会議種別:ポスター発表  

  26. Acinetobacter sp. Tol 5 の citral 生物変換に関わる代謝経路の同定

    宇佐見 享嗣, 石川 聖人, 堀 克敏

    第29回イソプレノイド研究会   2019年10月 

     詳細を見る

    会議種別:口頭発表(一般)  

  27. ハスモンヨトウ幼虫による分子ナノカーボンの生物変換

    宇佐見享嗣, 天池一真, 伊丹健一郎

    日本化学会 第101春季年会  2021年3月 

     詳細を見る

    会議種別:ポスター発表  

  28. Selective functionalization of molecular nanocarbon by Spodoptera litura

    Atsushi Usami, Kazuma Amaike, Kenichiro Itami

    The International Chemical Congress of Pacific Basin Societies (Pacifichem) 2021, Virtual symposium by Zoom  2021年12月20日 

     詳細を見る

    会議種別:口頭発表(一般)  

  29. Functionalization of cycloparaphenylene by biocatalysts

    Atsushi Usami, Kazuma Amaike, Katsutoshi Hori, Kenichiro Itami

    2022年3月26日 

     詳細を見る

    会議種別:口頭発表(一般)  

  30. Functionalization of Methylene-bridged [6]Cycloparaphenylene by Spodoptera litura

    Atsushi Usami, Hideya Kono, Hiroki Shudo, Akiko Yagi, Kazuma Amaike, Kenichiro Itami

    2022年6月29日 

     詳細を見る

    会議種別:ポスター発表  

  31. カチオン性ペリレンを用いた核酸輸送

    天池 一真, 加藤 江莉佳, 深津 美羽, Zetschok Dominik, 宇佐見 享嗣, 山田 早人, 伊丹 健一郎

    第16回バイオ関連化学シンポジウム  2022年9月11日 

     詳細を見る

    会議種別:口頭発表(一般)  

  32. 生物変換による酸素原子含有ナノベルトの合成

    河野英也, 宇佐見享嗣, 周戸大季, 天池一真, 八木, 亜樹子, 伊丹健一郎

    第32回基礎有機化学討論会  2022年9月21日 

     詳細を見る

    会議種別:ポスター発表  

  33. Acinetobacter sp. Tol 5 による citral の位置立体選択的生物変換と自然酸化

    宇佐見 享嗣, 石川 聖人, 堀 克敏

    第11回 バイオ関連化学シンポジウム  2017年9月 

     詳細を見る

  34. 農業害虫を益虫に。 招待有り

    宇佐見 享嗣

    九州・沖縄昆虫研究会2023年度秋の例会・第100回九州昆虫セミナー  2023年11月25日 

     詳細を見る

  35. 新奇環状ジアリールエーテルの合成と性質

    加藤智紀, 河野英也, 宇佐見享嗣, 周戸大季, 八木亜樹子, 伊丹健一郎

    第54回構造有機化学若手の会 夏の学校  2023年8月2日 

     詳細を見る

    会議種別:ポスター発表  

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  36. ハスモンヨトウ幼虫による分子ナノカーボンの生物変換

    宇佐見享嗣, 天池一真, 伊丹健一郎

    日本化学会 第101春季年会  2021年3月 

     詳細を見る

    会議種別:ポスター発表  

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  37. Selective functionalization of molecular nanocarbon by Spodoptera litura

    ○Atsushi Usami, Kazuma Amaike, Kenichiro Itami

    The International Chemical Congress of Pacific Basin Societies (Pacifichem) 2021, Virtual symposium by Zoom,  2021年12月20日 

     詳細を見る

    会議種別:ポスター発表  

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  38. Green bioproduction of monoterpenoid (E)-geranic acid from geraniol by genetically engineered Acinetobacter sp. Tol 5 in gas phase

    Atsushi Usami, Masahito Ishikawa, Katsutosi Hori

    8th Congress of European Microbiologists, FEMS  2019年7月 

     詳細を見る

    会議種別:ポスター発表  

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  39. Geraniol dehydrogenase の異種発現による Acinetobacter sp. Tol 5 の citral 生物変換に関わる代謝経路の同定

    宇佐見 享嗣, 石川 聖人, 堀 克敏

    日本農芸化学会2019年度大会  2019年3月 

     詳細を見る

  40. Functionalization of Methylene-bridged [6]Cycloparaphenylene by Spodoptera litura

    Atsushi Usami, Hideya Kono, Hiroki Shudo, Akiko Yagi, Kazuma Amaike, Kenichiro Itami

    第9回ITbM/GTRコンソーシアムワークショップ  2022年6月29日 

     詳細を見る

  41. Acinetobacter sp. Tol 5 を用いた citral の生体内変換と代謝経路の特定

    宇佐見 享嗣, 石川 聖人, 堀 克敏

    日本農芸化学会2018年度大会  2018年3月 

     詳細を見る

  42. Acinetobacter sp. Tol 5 の citral 生物変換に関わる代謝経路の同定

    宇佐見 享嗣, 石川 聖人, 堀 克敏

    第29回イソプレノイド研究会  2019年10月 

     詳細を見る

    会議種別:口頭発表(一般)  

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▼全件表示

その他研究活動 2

  1. Meet Ph.D candidate Atsushi Usami: FEMS2019 Congress Attendance Grantee

    2019年7月

     詳細を見る

    https://fems-microbiology.org/meet-ph-d-candidate-usami-atsushi-fems2019-congress-attendance-grantee/

  2. Meet Ph.D candidate Atsushi Usami: FEMS2019 Congress Attendance Grantee

    2019年5月

     詳細を見る

    https://fems-microbiology.org/meet-ph-d-candidate-usami-atsushi-fems2019-congress-attendance-grantee/

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共同研究・競争的資金等の研究課題 7

  1. ナノ物質材料に対する広食性昆虫ハスモンヨトウの生理的適応戦略に関する研究

    2023年7月 - 2024年6月

    公益財団法人 内藤科学技術振興財団  研究助成 

      詳細を見る

    担当区分:研究代表者 

  2. 昆虫を利用した機能性ナノカーボン材料の創製

    2023年4月 - 2024年3月

    公益財団法人 池谷科学技術振興財団   単年度研究助成 

      詳細を見る

    担当区分:研究代表者 

  3. 昆虫によるナノカーボン材料の機能化メカニズムの解明

    2023年4月 - 2024年3月

    公益財団法人中島記念国際交流財団   令和5年度日本人若手研究者研究助成 

      詳細を見る

    担当区分:研究代表者 

  4. 昆虫による分子ナノカーボンの生体内動態機構の解明

    2022年4月 - 2023年2月

    公益財団法人日本科学協会   笹川科学 学術研究部門  

      詳細を見る

    担当区分:研究代表者 

  5. 分子ナノカーボンを用いた定量的生物変換反応と生理活性評価

    2020年6月 - 2022年1月

    公益財団法人 立松財団   一般研究助成 

      詳細を見る

    担当区分:研究代表者 

  6. Green bioproduction of monoterpenoid (E)-geranic acid from geraniol by genetically engineered by Acinetobacter sp. Tol 5 in gas phase

    2019年7月

    The Federation of European Materials Societies FEMS Congress Attendance Grant  

      詳細を見る

    担当区分:研究代表者 

  7. Green bioproduction of monoterpenoid (E)-geranic acid from geraniol by genetically engineered by Acinetobacter sp. Tol 5 in gas phase

    2019年7月

    公益財団法人 加藤記念バイオサイエンス振興財団  第31回国際交流助成 

      詳細を見る

    担当区分:研究代表者 

▼全件表示

科研費 19

  1. 昆虫の異物代謝能力に着目した機能性ナノカーボン材料の開発

    2024年5月 - 2025年4月

    一般財団法人 東海産業技術振興財団  第36回 研究助成 

      詳細を見る

  2. 昆虫の生物機能を巧みに用いた資源循環型モノづくりの実現

    2024年4月 - 2029年3月

    文部科学省「世界で活躍できる研究者戦略育成事業」 世界的課題を解決する知の『開拓者』育成事業 国際・学際共同研究・産学連携 

      詳細を見る

    担当区分:研究代表者 

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  3. 昆虫由来の分子ナノカーボン代謝酵素の機能解析と新規機能性分子ナノカーボンの創製

    2024年4月 - 2027年3月

    日本学術振興会  科学研究費助成事業 若手研究 

      詳細を見る

    担当区分:研究代表者 

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  4. メダカの生理機能や行動を制御する共生細菌由来トリガー分子の特定

    2024年4月 - 2026年3月

    公益財団法人中部電気利用基礎研究振興財団  研究助成 A2 

    名古屋大学高等研究院/生命農学研究科特任助教 中山友哉

      詳細を見る

    担当区分:研究代表者 

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  5. バイオフィルムを制御するナノカーボン分子の開発と作用機構の解明

    2024年4月 - 2025年3月

    公益財団法人 中部科学技術センター  学術・みらい助成 

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    担当区分:研究代表者 

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  6. 炭素の導入と除去を利用した単金属の結晶構造ライブラリーの拡張

    2024年4月 - 2025年3月

    学際統合物質科学研究機構  令和6年度 学際統合物質科学研究機構「共創研究」研究費 

      詳細を見る

    担当区分:研究分担者 

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  7. 機能性分子ナノカーボン生産性を飛躍的に高めた生体触媒の作出

    2024年4月 - 2025年3月

    公益財団法人 岩谷直治記念財団  第50回 岩谷科学技術研究助成 

      詳細を見る

  8. 昆虫由来機能性分子ナノカーボンの生産プラットフォーム構築

    2024年3月 - 2025年5月

    一般財団法人材料科学技術振興財団  研究助成 

      詳細を見る

    担当区分:研究代表者 

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  9. ナノ物質材料に対する広食性昆虫ハスモンヨトウの生理的適応戦略に関する研究

    2023年7月 - 2024年6月

    公益財団法人 内藤科学技術振興財団  研究助成 

      詳細を見る

  10. 昆虫によるナノカーボン材料の機能化メカニズムの解明

    2023年4月 - 2024年3月

    公益財団法人中島記念国際交流財団  令和5年度日本人若手研究者研究助成 

    宇佐見享嗣

      詳細を見る

    担当区分:研究代表者 

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  11. 昆虫を利用した機能性ナノカーボン材料の創製

    2023年4月 - 2024年3月

    公益財団法人 池谷科学技術振興財団  単年度研究助成 

      詳細を見る

    担当区分:研究代表者 

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  12. 機能性ナノカーボン材料の高効率生産を指向した生体触媒の創製

    研究課題/研究課題番号:JPMJAX22B3  2022年10月 - 2025年3月

    科学技術振興機構  ACT-X 

      詳細を見る

    担当区分:研究代表者 

  13. 機能性ナノカーボン材料の高効率生産を指向した生体触媒の創製

    2022年10月 - 2025年3月

    国立研究開発法人 科学技術振興機構  ACT-X【野村 暢彦 研究総括】「環境とバイオテクノロジー」領域 

      詳細を見る

    担当区分:研究代表者 

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  14. 昆虫による分子ナノカーボンの直接官能基化に関わる新規タンパク質の探索と機能解明

    研究課題/研究課題番号:22K14782  2022年4月 - 2024年3月

    科学研究費助成事業  若手研究

    宇佐見 享嗣

      詳細を見る

    担当区分:研究代表者 

    配分額:4550000円 ( 直接経費:3500000円 、 間接経費:1050000円 )

    分子ナノカーボンは、最も活発な研究領域の一つであり、官能基を付与することで新たな物性や機能が見出されている。しかし、適用可能な分子は限定的なため、依然として反応開発が強く求められている。最近申請者は、代表的な分子ナノカーボンであるカーボンナノリング ([6]CPP) がハスモンヨトウ幼虫により位置選択的に直接水酸化されることを発見した。そこで本研究では、 [6]CPP の水酸化反応に関わる遺伝子を RNA-seq により探索・同定し、同遺伝子にコードされるタンパク質の機能を明らかにすることで基礎と応用の両面からナノカーボンバイオロジーという新分野の創出に向けた学術的な基盤を固める。
    ナノメートルサイズの単一多環芳香族炭化水素である分子ナノカーボンは、最も活発な研究領域の一つであり、官能基を付与することで新たな物性や機能が見出されている。しかし、位置選択的に直接官能基を付与できる分子ナノカーボンは限定的なため、依然として反応開発が強く求められている。最近申請者は、代表的な分子ナノカーボンであるカーボンナノリング ([6]CPP) が昆虫であるハスモンヨトウ幼虫により位置選択的に直接酸化されることを発見した。そこで本研究では、 [6]CPP の酸化反応に関わる遺伝子を RNA-seq により探索・同定し、同遺伝子にコードされるタンパク質の機能を明らかにすることを目的とした。
    まず、ハスモンヨトウ幼虫の[6]CPPに対する摂食阻害活性を評価し、摂食阻害を示さない最大濃度を見出した。続いて、見出した最大濃度の人工飼料を用いた生物変換を実施し、代謝物から有機溶媒により抽出・精製することで[6]CPP 誘導体の生産効率を算出した。また、生物変換に用いたハスモンヨトウ幼虫の中腸からRNAを抽出し、RNA-seqを実施し、[6]CPPを与えていない個体と比較して特定の遺伝子群が発現量増加したことを確認した。確認した遺伝子群の中でも、異物代謝への関与が示唆される遺伝子を標的にしたノックダウン個体をRNAi法により作出した。作出した個体を用いた生物変換を実施することで [6]CPP誘導体の生産量減少を確認した。
    研究計画著書の計画通りにおおむね研究が進んだ。特に、RNA-seqを実施したことで標的とする遺伝子候補を見出すことができた。
    標的遺伝子候補にコードされるタンパク質の機能を明らかにするため、異種タンパク質発現系による機能解析検討を行う。

  15. 昆虫による分子ナノカーボンの生体内動態機構の解明

    2022年4月 - 2023年2月

    公益財団法人日本科学協会 笹川科学  学術研究部門 

    宇佐見享嗣

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    担当区分:研究代表者 

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  16. 分子ナノカーボンを用いた定量的生物変換反応と生理活性評価

    2020年8月 - 2022年1月

    公益財団法人立松財団  一般研究助成 

    宇佐見享嗣

      詳細を見る

    担当区分:研究代表者 

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  17. Green bioproduction of monoterpenoid (E)-geranic acid from geraniol by genetically engineered by Acinetobacter sp. Tol 5 in gas phase

    2019年7月

    The Federation of European Materials Societies  FEMS Congress Attendance Grant 

    Atsushi Usami

      詳細を見る

    担当区分:研究代表者 

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  18. Green bioproduction of monoterpenoid (E)-geranic acid from geraniol by genetically engineered by Acinetobacter sp. Tol 5 in gas phase

    2019年7月

    公益財団法人 加藤記念バイオサイエンス振興財団  国際交流助成 

    宇佐見 享嗣

      詳細を見る

    担当区分:研究代表者 

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  19. 揮発性化合物の高濃度高効率生産に向けた気相微生物反応の構築

    研究課題/研究課題番号:18J15181  2018年4月 - 2020年3月

    日本学術振興会  科学研究費助成事業 特別研究員奨励費  特別研究員奨励費

    宇佐見 享嗣, 宇佐見 享嗣

      詳細を見る

    担当区分:その他 

    本研究の目的は有用揮発性化合物を高濃度かつ、高効率で生産する気相微生物反応プロセスを構築することである。モデル反応系として、ゲラニオールからトランス-ゲラン酸に気相変換するプロセスを作る。ゲラニオール、トランス-ゲラン酸は揮発性化合物であることに加え、水溶性が極めて低く、液相反応による物質変換は極めて困難である。微生物触媒は担体へ固定し、基質ゲラニオールは気体で供給する。そして、生産物トランス-ゲラン酸は菌体から気体として回収する。目的達成のため、高付着性と高有機溶媒耐性を併せ持つAcinetobacter sp. Tol5を用いてトランス-ゲラン酸の高生産微生物触媒を創出し、気相微生物反応プロセスを構築する。平成30年度は、トランス-ゲラン酸の高生産微生物触媒の創出を実施した。
    (1)目的生産物であるトランス-ゲラン酸および中間体であるゲラニアールに対するTol5株の耐性評価:既報より4倍以上の濃度に耐えられることが明らかとなった。
    (2)ゲラニオール/トランス-ゲラン酸変換の確認:Tol5株にはゲラニオールに対して変換能力を持たないことを明らかにした。そこで変換能力を付与するため、人工合成遺伝子を用いて導入した。得られたTol5形質転換体が機能するか、バッチの液相反応系にて確認するため、GC-MSを用いて継時的に評価したところ、Tol5形質転換体は2種の化合物を微量生産した。それらを単離・精製し解析した結果、トランス-ゲラン酸と(1R,3R,4R)-1-メチル-4-(1-メチルエテニル)-1,3-シクロヘキサンジオールであることが明らかとなった。
    (3)トランス-ゲラン酸高生産 Tol5株の創出:配列類似性解析によりTol5のゲノムから見出したホモログを欠損し、変異体を取得した。得られた変異体はトランス-ゲラン酸高生産株であることが明らかとなった。

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