Organization
Graduate School of Science Designated Assistant Professor
Title
Designated Assistant Professor
HIRAOKA Haruka
Degree 3
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Doctor (Science) （ 2020.3 Osaka University ）
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Master (Science) （ 2016.3 Osaka University ）
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Bachelor (Science) （ 2014.3 Osaka University ）
Papers 11
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Cell–cell heterogeneity in phosphoenolpyruvate carboxylase biases early cell fate priming in Dictyostelium discoideum Reviewed Open Access
Kenichi Abe, Hidenori Hashimura, Haruka Hiraoka, Shoko Fujishiro, Narufumi Kameya, Kazuteru Taoka, Satoshi Kuwana, Masashi Fukuzawa, Satoshi Sawai
Frontiers in Cell and Developmental Biology Vol. 12 2025.2
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Intracellular Delivery of Antisense Oligonucleotides by Tri-Branched Cyclic Disulfide Units Reviewed Open Access
Lyu, FJ; Hakariya, H; Hiraoka, H; Li, ZM; Matsubara, N; Soo, Y; Hashiya, F; Abe, N; Shu, ZM; Nakamoto, K; Kimura, Y; Abe, H
CHEMMEDCHEM Vol. 19 ( 20 ) page： e202400472 2024.7
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Nomura, K; Onda, K; Murase, H; Hashiya, F; Ono, Y; Terai, G; Oka, N; Asai, K; Suzuki, D; Takahashi, N; Hiraoka, H; Inagaki, M; Kimura, Y; Shimizu, Y; Abe, N; Abe, H
RSC CHEMICAL BIOLOGY 2024.2
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Topological capture of mRNA for silencing gene expression Reviewed Open Access
Lyu Fangjie, Tomita Takashi, Abe Naoko, Hiraoka Haruka, Hashiya Fumitaka, Nakashima Yuko, Kajihara Shiryu, Tomoike Fumiaki, Shu Zhaoma, Onizuka Kazumitsu, Kimura Yasuaki, Abe Hiroshi
CHEMICAL COMMUNICATIONS Vol. 59 ( 77 ) page： 11564 - 11567 2023.9
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The Effect of γ Phosphate Modified Deoxynucleotide Substrates on PCR Activity and Fidelity Reviewed Open Access
Hashiya Fumitaka, Murase Hirotaka, Chandela Akash, Hiraoka Haruka, Inagaki Masahito, Nakashima Yuko, Abe Naoko, Nakamura Mayu, Terai Goro, Kimura Yasuaki, Ando Kaori, Oka Natsuhisa, Asai Kiyoshi, Abe Hiroshi
CHEMBIOCHEM Vol. 24 ( 14 ) page： e202200572 2023.6
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Cap analogs with a hydrophobic photocleavable tag enable facile purification of fully capped mRNA with various cap structures Reviewed Open Access
Inagaki Masahito, Abe Naoko, Li Zhenmin, Nakashima Yuko, Acharyya Susit, Ogawa Kazuya, Kawaguchi Daisuke, Hiraoka Haruka, Banno Ayaka, Meng Zheyu, Tada Mizuki, Ishida Tatsuma, Lyu Pingxue, Kokubo Kengo, Murase Hirotaka, Hashiya Fumitaka, Kimura Yasuaki, Uchida Satoshi, Abe Hiroshi
NATURE COMMUNICATIONS Vol. 14 ( 1 ) page： 2657 2023.5
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Creation of single molecular conjugates of metal-organic cages and DNA Reviewed Open Access
Nakajo Toshinobu, Kusaka Shinpei, Hiraoka Haruka, Nomura Kohei, Matsubara Noriaki, Baba Rintaro, Yoshida Yuki, Nakamoto Kosuke, Honma Masakazu, Iguchi Hiroaki, Uchihashi Takayuki, Abe Hiroshi, Matsuda Ryotaro
CHEMICAL COMMUNICATIONS Vol. 59 ( 33 ) page： 4974 - 4977 2023.4
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ATP levels influence cell movement during the mound phase in Dictyostelium discoideum as revealed by ATP visualization and simulation Reviewed Open Access
Hiraoka Haruka, Wang Jiewen, Nakano Tadashi, Hirano Yasuhiro, Yamazaki Shinichi, Hiraoka Yasushi, Haraguchi Tokuko
FEBS OPEN BIO Vol. 12 ( 11 ) page： 2042 - 2056 2022.11
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A 2 & PRIME;-modified uridine analog, 2'-O-(methylthiomethoxy)methyl uridine, for siRNA applications Reviewed International coauthorship Open Access
Lyu Fangjie, An Seongjin, Kobayashi Yoshiaki, Nomura Kohei, Baba Rintaro, Abe Naoko, Hiraoka Haruka, Hashiya Fumitaka, Shu Zhaoma, Ui-Tei Kumiko, Kimura Yasuaki, Abe Hiroshi
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS Vol. 74 page： 128939 2022.10
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Antisense Oligonucleotide Modified with Disulfide Units Induces Efficient Exon Skipping in <i>mdx</i> Myotubes through Enhanced Membrane Permeability and Nucleus Internalization Reviewed International coauthorship
Hiraoka Haruka, Shu Zhaoma, Tri Le Bao, Masuda Keiko, Nakamoto Kosuke, Fangjie Lyu, Abe Naoko, Hashiya Fumitaka, Kimura Yasuaki, Shimizu Yoshihiro, Veedu Rakesh N., Abe Hiroshi
CHEMBIOCHEM Vol. 22 ( 24 ) page： 3437 - 3442 2021.12
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Intracellular ATP levels influence cell fates in <i>Dictyostelium discoideum</i> differentiation Reviewed Open Access
Hiraoka Haruka, Nakano Tadashi, Kuwana Satoshi, Fukuzawa Masashi, Hirano Yasuhiro, Ueda Masahiro, Haraguchi Tokuko, Hiraoka Yasushi
GENES TO CELLS Vol. 25 ( 5 ) page： 312 - 326 2020.5
Books 3
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核酸医薬・mRNA医薬の製造分析の基礎と基盤技術開発
乙竹真美、平岡陽花、阿部洋（ Role： Contributor , 翻訳反応を最大化するための合成mRNAの分子設計）
CMCリサーチ 2023.2
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総合診療コラム「mRNAワクチンの現在と未来」
平岡 陽花
医学書院 2023
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最先端ナノライフシステム研究
平岡陽花、阿部洋（ Role： Contributor , ジスルフィド修飾による核酸の機能向上と医薬応用）
2022 （ ISBN:9784863455207 ）
Research Project for Joint Research, Competitive Funding, etc. 3
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がん抑制型核酸医薬開発
2025.4 - 2028.3
若手新分野創成研究ユニット
平岡陽花, 橋谷文貴
Authorship：Principal investigator Grant type：Competitive
Grant amount：\3000000 （ Direct Cost: \3000000 、 Indirect Cost：\3000000 ）
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貪食マクロファージと腸上皮細胞間の相互作用が導く寿命延伸の機構解明
2025.4 - 2026.3
公益財団法人 堀科学芸術振興財団 研究助成（第一部）
平岡陽花
Authorship：Principal investigator Grant type：Competitive
Grant amount：\2000000 （ Direct Cost: \2000000 ）
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貪食マクロファージが腸で発揮する寿命延伸機構の遺伝的基盤の解明
2024.11 - 2026.9
公益財団法人 住友財団 基礎科学研究助成
平岡陽花, 大澤志津江
Authorship：Principal investigator Grant type：Competitive
Grant amount：\4500000 （ Direct Cost: \4500000 ）
KAKENHI (Grants-in-Aid for Scientific Research) 3
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細胞間力の可視化による「細胞接着が駆動する細胞競合」のメカニズム解明
Grant number：24K18111 2024.4 - 2029.3
科学研究費助成事業 若手研究
平岡 陽花
Authorship：Principal investigator
Grant amount：\4550000 （ Direct Cost: \3500000 、 Indirect Cost：\1050000 ）
がん抑制性のHippo経路が不活化したfat変異細胞群は、隣接する正常細胞を排除して自身の領域を拡大するスーパーコンペティションを起こし、がんの悪性化を招く。我々は、周辺の正常細胞に対して接着因子であるed遺伝子の変異を導入することで、fat変異細胞によるスーパーコンペティションが著しく促進されることを見出した。本研究課題では、ed変異がもたらす影響の理解を通じて、fat変異細胞によるスーパーコンペティションの分子機構を明らかにすることを目指す。
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Development of membrane-permeable oligo nucleotide and clarification of the uptake mechanism
Grant number：21K14750 2021.4 - 2024.3
Grants-in-Aid for Scientific Research Grant-in-Aid for Early-Career Scientists
Authorship：Principal investigator
Grant amount：\4420000 （ Direct Cost: \3400000 、 Indirect Cost：\1020000 ）
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Visualization of the intracellular dynamics of artificial mRNAs
Grant number：20K22704 2020.9 - 2022.3
Grants-in-Aid for Scientific Research Grant-in-Aid for Research Activity Start-up
Hiraoka Haruka
Authorship：Principal investigator
Grant amount：\2860000 （ Direct Cost: \2200000 、 Indirect Cost：\660000 ）
In this research theme, I tried to develop the RNA imaging method using fluorescence nucleic acid probe. The nucleic acid probe used for bacteria in previous research could not be used in human cells as such short oligonucleotide easily permeated into nuclei and rarely bound with target mRNA in cytosol. Therefore, instead of using the nucleic acid probe, I currently deal with the development of fluorescence mRNA composed with fluorescence nucleotides. I also achieved the intracellular delivery of the nucleic acid probe by chemical modification. Previously developed membrane-permeable oligonucleotide including 5 repeats of disulfide modification units has limited use due to their high hydrophobicity. This study reported circular disulfide group as promising modification; it enables the efficient intracellular delivery with smaller number of units.