Organization
Graduate School of Science Designated assistant professor
Title
Designated assistant professor
HIRAOKA Haruka
Degree 3
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Doctor (Science) （ 2020.3 Osaka University ）
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Master (Science) （ 2016.3 Osaka University ）
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Bachelor (Science) （ 2014.3 Osaka University ）
Papers 10
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Nomura, K; Onda, K; Murase, H; Hashiya, F; Ono, Y; Terai, G; Oka, N; Asai, K; Suzuki, D; Takahashi, N; Hiraoka, H; Inagaki, M; Kimura, Y; Shimizu, Y; Abe, N; Abe, H
RSC CHEMICAL BIOLOGY 2024.2
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Topological capture of mRNA for silencing gene expression Reviewed
Lyu Fangjie, Tomita Takashi, Abe Naoko, Hiraoka Haruka, Hashiya Fumitaka, Nakashima Yuko, Kajihara Shiryu, Tomoike Fumiaki, Shu Zhaoma, Onizuka Kazumitsu, Kimura Yasuaki, Abe Hiroshi
CHEMICAL COMMUNICATIONS Vol. 59 ( 77 ) page： 11564 - 11567 2023.9
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The Effect of γ Phosphate Modified Deoxynucleotide Substrates on PCR Activity and Fidelity Reviewed
Hashiya Fumitaka, Murase Hirotaka, Chandela Akash, Hiraoka Haruka, Inagaki Masahito, Nakashima Yuko, Abe Naoko, Nakamura Mayu, Terai Goro, Kimura Yasuaki, Ando Kaori, Oka Natsuhisa, Asai Kiyoshi, Abe Hiroshi
CHEMBIOCHEM Vol. 24 ( 14 ) page： e202200572 2023.6
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Inagaki Masahito, Abe Naoko, Li Zhenmin, Nakashima Yuko, Acharyya Susit, Ogawa Kazuya, Kawaguchi Daisuke, Hiraoka Haruka, Banno Ayaka, Meng Zheyu, Tada Mizuki, Ishida Tatsuma, Lyu Pingxue, Kokubo Kengo, Murase Hirotaka, Hashiya Fumitaka, Kimura Yasuaki, Uchida Satoshi, Abe Hiroshi
NATURE COMMUNICATIONS Vol. 14 ( 1 ) page： 2657 2023.5
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Creation of single molecular conjugates of metal-organic cages and DNA Reviewed
Nakajo Toshinobu, Kusaka Shinpei, Hiraoka Haruka, Nomura Kohei, Matsubara Noriaki, Baba Rintaro, Yoshida Yuki, Nakamoto Kosuke, Honma Masakazu, Iguchi Hiroaki, Uchihashi Takayuki, Abe Hiroshi, Matsuda Ryotaro
CHEMICAL COMMUNICATIONS Vol. 59 ( 33 ) page： 4974 - 4977 2023.4
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特集 COVID-19パンデミック 振り返りと将来への備え 【コラム】 ❷mRNAワクチンの現在と未来 Invited
平岡 陽花, 阿部 洋
総合診療 Vol. 33 ( 1 ) page： 84 - 86 2023.1
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Hiraoka Haruka, Wang Jiewen, Nakano Tadashi, Hirano Yasuhiro, Yamazaki Shinichi, Hiraoka Yasushi, Haraguchi Tokuko
FEBS OPEN BIO Vol. 12 ( 11 ) page： 2042 - 2056 2022.11
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A 2 & PRIME;-modified uridine analog, 2'-O-(methylthiomethoxy)methyl uridine, for siRNA applications Reviewed International coauthorship
Lyu Fangjie, An Seongjin, Kobayashi Yoshiaki, Nomura Kohei, Baba Rintaro, Abe Naoko, Hiraoka Haruka, Hashiya Fumitaka, Shu Zhaoma, Ui-Tei Kumiko, Kimura Yasuaki, Abe Hiroshi
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS Vol. 74 page： 128939 2022.10
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Antisense Oligonucleotide Modified with Disulfide Units Induces Efficient Exon Skipping in <i>mdx</i> Myotubes through Enhanced Membrane Permeability and Nucleus Internalization Reviewed International coauthorship
Hiraoka Haruka, Shu Zhaoma, Tri Le Bao, Masuda Keiko, Nakamoto Kosuke, Fangjie Lyu, Abe Naoko, Hashiya Fumitaka, Kimura Yasuaki, Shimizu Yoshihiro, Veedu Rakesh N., Abe Hiroshi
CHEMBIOCHEM Vol. 22 ( 24 ) page： 3437 - 3442 2021.12
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Intracellular ATP levels influence cell fates in <i>Dictyostelium discoideum</i> differentiation Reviewed
Hiraoka Haruka, Nakano Tadashi, Kuwana Satoshi, Fukuzawa Masashi, Hirano Yasuhiro, Ueda Masahiro, Haraguchi Tokuko, Hiraoka Yasushi
GENES TO CELLS Vol. 25 ( 5 ) page： 312 - 326 2020.5
Books 2
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核酸医薬・mRNA医薬の製造分析の基礎と基盤技術開発
乙竹真美、平岡陽花、阿部洋（ Role： Contributor , 翻訳反応を最大化するための合成mRNAの分子設計）
CMCリサーチ 2023.2
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最先端ナノライフシステム研究
平岡陽花、阿部洋（ Role： Contributor , ジスルフィド修飾による核酸の機能向上と医薬応用）
2022 （ ISBN:9784863455207 ）
KAKENHI (Grants-in-Aid for Scientific Research) 2
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Development of membrane-permeable oligo nucleotide and clarification of the uptake mechanism
Grant number：21K14750 2021.4 - 2024.3
Grants-in-Aid for Scientific Research Grant-in-Aid for Early-Career Scientists
Authorship：Principal investigator
Grant amount：\4420000 （ Direct Cost: \3400000 、 Indirect Cost：\1020000 ）
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Visualization of the intracellular dynamics of artificial mRNAs
Grant number：20K22704 2020.9 - 2022.3
Grants-in-Aid for Scientific Research Grant-in-Aid for Research Activity Start-up
Hiraoka Haruka
Authorship：Principal investigator
Grant amount：\2860000 （ Direct Cost: \2200000 、 Indirect Cost：\660000 ）
In this research theme, I tried to develop the RNA imaging method using fluorescence nucleic acid probe. The nucleic acid probe used for bacteria in previous research could not be used in human cells as such short oligonucleotide easily permeated into nuclei and rarely bound with target mRNA in cytosol. Therefore, instead of using the nucleic acid probe, I currently deal with the development of fluorescence mRNA composed with fluorescence nucleotides. I also achieved the intracellular delivery of the nucleic acid probe by chemical modification. Previously developed membrane-permeable oligonucleotide including 5 repeats of disulfide modification units has limited use due to their high hydrophobicity. This study reported circular disulfide group as promising modification; it enables the efficient intracellular delivery with smaller number of units.