記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1113/jp280066

DOI： 10.1113/JP280066

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1038/s41467-019-12478-x

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1016/j.celrep.2019.04.040

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記述言語：日本語   出版者・発行元：公益社団法人 日本農芸化学会  

<p>食と健康の関係が注目される現在，食品は味や匂いなどの「感覚」・「栄養」・「生体調節」の3つの機能を兼ね備えることが知られるようになった．また，神経科学研究の進展により，摂食行動は嗜好性と恒常性（体の状態を一定に保つ性質）により制御されることがわかってきた．食品機能のうち，「感覚」は前者にかかわるのに対し，「栄養」や「生体調節」は後者にかかわる．これら2つの仕組みは食事の際に同時に働くが，食品研究と神経研究の分野間には隔たりがあり，食品機能性と摂食制御の関係の把握は困難である．本稿では，食の嗜好性と恒常性のセンシング機構を説明するとともに，今後，食品研究を行ううえでも重要になると思われるトピックについて述べる．</p>

DOI： 10.1271/kagakutoseibutsu.56.255

CiNii Research

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1038/ncomms10268

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1038/srep12947

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1007/978-1-4939-2914-6_14

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掲載種別：研究論文（学術雑誌）  

The release of insulin from pancreatic β-cells is regulated by a considerable number of G protein-coupled receptors. During the past several years, we have focused on the physiological importance of β-cell M3 muscarinic acetylcholine receptors (M3Rs). At the molecular level, the M3R selectively activates G proteins of the G(q) family. Phenotypic analysis of several M3R mutant mouse models, including a mouse strain that lacks M3Rs only in pancreatic β-cells, indicated that β-cell M3Rs play a key role in maintaining blood glucose levels within a normal range. Additional studies with transgenic M3R mouse models strongly suggest that strategies aimed to enhance signaling through β-cell M3Rs may prove useful in the treatment of type 2 diabetes. More recently, we analyzed transgenic mice that expressed an M3R-based designer receptor in a β-cell-specific fashion, which enabled us to chronically activate a β-cell G(q)-coupled receptor by a drug that is otherwise pharmacologically inert. Drug-dependent activation of this designer receptor stimulated the sequential activation of G(q), phospholipase C, ERK1/2, and insulin receptor substrate 2 signaling, thus triggering a series of events that greatly improved β-cell function. Most importantly, chronic stimulation of this pathway protected mice against experimentally induced diabetes and glucose intolerance, induced either by streptozotocin or by the consumption of an energy-rich, high-fat diet. Because β-cells are endowed with numerous receptors that mediate their cellular effects via activation of G(q)-type G proteins, these findings provide a rational basis for the development of novel antidiabetic drugs targeting this class of receptors.

DOI： 10.1210/me.2013-1084

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掲載種別：研究論文（学術雑誌）  

Muscarinic receptor-based designer receptors have emerged as powerful novel tools to study G-protein-coupled receptor (GPCR) signaling and physiology. These new designer GPCRs, which are most frequently referred to as DREADDs (designer receptors exclusively activated by designer drug), are unable to bind acetylcholine, the endogenous muscarinic receptor agonist, but can be activated by clozapine-N-oxide (CNO), an otherwise pharmacologically inert compound, with high potency and efficacy. The various DREADDs differ primarily in their G protein coupling preference. More recently, an arrestin-biased DREADD has also been developed. The expression of DREADDs in distinct tissues or cell types has enabled researchers to study the outcome of selective stimulation of distinct GPCR (or arrestin) signaling pathways in a temporally and spatially controlled fashion in vivo. In this review, we provide an up-to-date snapshot of where this field currently stands and which important novel insights have been gained using this new technology.

DOI： 10.1016/j.tips.2013.04.006

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1124/mol.112.080358

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1096/fj.12-204644

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1371/journal.pone.0035380

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1073/pnas.1016644108

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Japan Society for Bioscience, Biotechnology, and Agrochemistry  

Neoculin, a sweet protein found in the fruit of <I>Curculigo latifolia</I>, has the ability to change sourness into sweetness. Neoculin turns drinking water sweet, indicating that non-acidic compounds may induce the sweetness. We report that ammonium chloride and certain amino acids elicit the intense sweetness of neoculin. Neoculin can thus sweeten amino acid-enriched foods.

DOI： 10.1271/bbb.110081

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その他リンク： https://jlc.jst.go.jp/DN/JALC/00375648487?from=CiNii

記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：WILEY-BLACKWELL  

We have developed a device that analyses the interaction of food components with model epithelial cells using surface plasmon resonance (SPR). A model of epithelial lingual cells was devised using a liposome composed of a mixture of four phospholipids. The liposome was immobilised to the L1 sensor tip attached to the sensor port of the SPR system. The interaction of food components with the model lingual epithelial cells was determined by the patterns of sensorgrams. According to this method, food components were classified into three groups: group A, strong interaction with the lipid bilayer; group B, weak interaction; and group C, neither A-nor B-type interaction. The sensorgrams of group A showed gradual binding and slow dissociation from the surface of the lipid bilayer. In group B, the food components showed rapid binding and rapid dissociation from the lipid bilayer. The compounds in group C exhibited weak binding to the lipid bilayer, but parts of these samples formed rigid complexes with the lipid bilayer. Sweet proteins producing prolonged sweetness perception as well as miraculin with its taste-modifying activity were classified into group A. The sensory activities of these substances are probably induced by their strong interactions with the epithelial cell surface; they have distinct binding constants with the lipid bilayer. Thaumatin exhibited the strongest interaction, followed by monellin and miraculin. Copyright (C) 2011 John Wiley & Sons, Ltd.

DOI： 10.1002/ffj.2073

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1371/journal.pone.0019448

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CiNii Research

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1021/jf102573w

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Springer Science+Business Media B.V.  

PURPOSE OF WORK: Soluble protein expression is an important first step during various types of protein studies. Here, we present the screening strategy of secretable mutant. The strategy aimed to identify those cysteine residues that provoke protein misfolding in the heterologous expression system. Intentional mutagenesis studies should consider the size of the library and the time required for expression screening. Here, we proposed a cysteine-to-serine shuffling mutation strategy (CS shuffling) using a Saccharomyces cerevisiae expression system. This strategy of site-directed shuffling mutagenesis of cysteine-to-serine residues aims to identify the cysteine residues that cause protein misfolding in heterologous expression. In the case of a nonglycosylated mutant of the taste-modifying protein miraculin (MCL), which was used here as a model protein, 25% of all constructs obtained from CS shuffling expressed MCL mutant, and serine mutations were found at Cys47 or Cys92, which are involved in the formation of the disulfide bond. This indicates that these residues had the potential to provoke protein misfolding via incorrect disulfide bonding. The CS shuffling can be performed using a small library and within one week, and is an effective screening strategy of soluble protein expression.

DOI： 10.1007/s10529-010-0399-1

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1016/j.bbagen.2010.06.003

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Japan Society for Bioscience, Biotechnology, and Agrochemistry  

Neoculin has pH-dependent taste-modifying activity. This study found that neoculin changed pH-dependently in its tryptophan- and ANS-derived fluorescence spectra, while no such change occurred in a neoculin variant whose histidine residues were replaced with alanine. These results suggest that the sweetness of neoculin depends on structural change accompanying the pH change, with the histidine residues playing a key role.

DOI： 10.1271/bbb.90524

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その他リンク： https://jlc.jst.go.jp/DN/JALC/00340708765?from=CiNii

記述言語：日本語  

PubMed

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1021/jf800214b

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1096/fj.07-100289

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記述言語：英語   掲載種別：研究論文（国際会議プロシーディングス）   出版者・発行元：AMER CHEMICAL SOC  

The majority of sweet compounds are of low-molecular-weight, but several proteins elicit sweet taste responses in humans. The fruit of Curculigo latifolia has been known to contain a protein that has both sweetness and a taste-modifying activity to convert sourness to sweetness. Recently, we have purified and re-identified the active component to reveal that it is a heterodimeric protein named "neoculin". The result of Xray crystallographic analysis has indicated that the overall structure of neoculin is similar to those of monocot mannose-binding lectins, while there is little structural similarity between neoculin and structure-solved sweet proteins. Direct interaction between neoculin and human sweet taste receptor hT1R2-hT1R3 has been indicated by response of HEK293T cells expressing this receptor, and by the inhibition of neoculin activity with lactisole, a hT1R2-hT1R3 blocker. Combining the results of molecular dynamics simulations and docking model generation between neoculin and hT1R2-hT1R3, we propose a hypothesis that neoculin is in dynamic equilibrium between "open" and "closed" states, and that the addition of an acid shifts the equilibrium to the "open" state for easier fitting to the receptor.

DOI： 10.1021/bk-2008-0979.ch035

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その他リンク： http://orcid.org/0000-0002-9547-5767

出版者・発行元：公益財団法人 日本醸造協会  

熱帯植物クルクリゴの果実中に含まれるネオクリンは, 酸味を強い甘味に変換するユニークな味覚修飾活性を持つ。この不思議な性質は味覚サイエンスの大きなトピックの一つであるが, ネオクリンを大量に生産することが困難であった。そのため麹菌による生産が試みられ, 天然と同等の活性をもつネオクリンの生産に成功した。麹菌の新たな活躍の場として注目される成果である。

DOI： 10.6013/jbrewsocjapan1988.103.586

CiNii Research

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1016/j.bbrc.2007.06.064

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1016/j.bbrc.2007.04.171

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1097/01.wnr.0000230513.01339.3b

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1128/aem.72.5.3716-3723.2006

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1016/j.jmb.2006.03.030

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Japan Society for Bioscience, Biotechnology, and Agrochemistry  

A unique taste-modifying activity that converts the sense of sourness to the sense of sweetness occurs in the fruit of the plant <I>Curculigo latifolia</I>, intrinsic to West Malaysia. The active component, known as curculin, is a protein consisting of two identical subunits. We have found a new taste-modifying protein, named neoculin, of the same origin. Both chemical analysis and cDNA cloning characterized neoculin as a heterodimeric protein consisting of an acidic, glycosylated subunit of 113 amino acid residues and a basic subunit that is the monomeric curculin itself.

DOI： 10.1271/bbb.68.1403

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その他リンク： https://jlc.jst.go.jp/DN/JALC/00238770270?from=CiNii

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記述言語：英語   掲載種別：研究発表ペーパー・要旨（国際会議）   出版者・発行元：OXFORD UNIV PRESS  

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開催年月日： 2023年3月

記述言語：日本語   会議種別：口頭発表（一般）  

開催年月日： 2023年3月

記述言語：英語   会議種別：ポスター発表  

開催年月日： 2023年3月

記述言語：英語   会議種別：シンポジウム・ワークショップ パネル（指名）  

開催年月日： 2023年1月

記述言語：日本語   会議種別：公開講演，セミナー，チュートリアル，講習，講義等  

開催地：岡崎市公衆衛生センター  

開催年月日： 2022年12月

記述言語：英語   会議種別：ポスター発表  

開催年月日： 2022年12月

記述言語：英語   会議種別：シンポジウム・ワークショップ パネル（指名）