Updated on 2022/05/18

写真a

 
IIJIMA Hirotaka
 
Organization
Institute for Advanced Research Designated assistant professor
Graduate School of Medicine Designated assistant professor
Title
Designated assistant professor

Degree 2

  1. 修士(人間健康科学) ( 2014.3   京都大学 ) 

  2. Doctor (Human Health Sciences) ( 2017.3   Kyoto University ) 

Research Interests 4

  1. Systems Biology

  2. Mechanobiology

  3. Aging

  4. Rehabilitation

Research Areas 4

  1. Life Science / Orthopedics

  2. Life Science / Biomedical engineering

  3. Life Science / Molecular biology

  4. Life Science / Rehabilitation science

Current Research Project and SDGs 3

  1. マルチセンサの有機的統合による革新的な運動機能評価システムの構築

  2. New Research Field Rejuvenative Rehabilitation

  3. Investigation of Epigenetic Regulatory Mechanism Driving Age-related Diseases

Research History 6

  1. Nagoya University   Institute for Advanced Research / Graduate School of Medicine   Assistant Professor

    2022.4

      More details

    Country:Japan

  2. University of Pittsburgh   School of Medicine, Physical Medicine and Rehabilitation (Paid Visiting Scholar, The Uehara Memorial Foundation))   研究員

    2021.4 - 2022.3

  3. Keio University   Researcher

    2019.5 - 2022.3

  4. University of Pittsburgh   School of Medicine, Physical Medicine and Rehabilitation (Visiting Scholar, JSPS Overse Research Fellow)   研究員

    2019.5 - 2021.3

  5. Kyoto University   Researcher

    2017.4 - 2021.3

  6. Keio University   Researcher

    2017.4 - 2019.4

▼display all

Professional Memberships 5

  1. 公益社団法人 日本理学療法士協会

  2. 日本軟骨代謝学会

  3. Osteoarthritis Research Society International (OARSI)

  4. 一般社団法人 日本機械学会

  5. American PHysiological Society

Awards 6

  1. Research Fellowship

    2021.4   The Uehara Memorial Foundation  

  2. Young Investigator Award Osteoarthritis Research Society International

    2020.5  

  3. Travel Award for 8th International Symposium on Regenerative Rehabilitation Alliance for Regenerative Rehabilitation Research and Training

    2019.10  

  4. 日本長寿科学振興財団国際学会派遣事業 公益財団法人長寿科学振興財団

    2018.4  

  5. 1st Author 日本骨代謝学会

    2017.3  

  6. Outstanding Contribution in Reviewing Journal - Osteoarthritis Cartilage Elsevier

    2016  

▼display all

 

Papers 83

  1. Meta-analysis integrated with multi-omics data analysis to elucidate pathogenic mechanisms of age-related knee osteoarthritis in mice. International journal

    Hirotaka Iijima, Gabrielle Gilmer, Kai Wang, Sruthi Sivakumar, Christopher Evans, Yusuke Matsui, Fabrisia Ambrosio

    The journals of gerontology. Series A, Biological sciences and medical sciences     2022.1

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    Increased mechanistic insight into the pathogenesis of knee osteoarthritis (KOA) is needed to develop efficacious disease-modifying treatments. Though age-related pathogenic mechanisms are most relevant to the majority of clinically-presenting KOA, the bulk of our mechanistic understanding of KOA has been derived using surgically induced post-traumatic OA (PTOA) models. Here, we took an integrated approach of meta-analysis and multi-omics data analysis to elucidate pathogenic mechanisms of age-related KOA in mice. Protein-level data were integrated with transcriptomic profiling to reveal inflammation, autophagy, and cellular senescence as primary hallmarks of age-related KOA. Importantly, the molecular profiles of cartilage aging were unique from those observed following PTOA, with less than 3% overlap between the two models. At the nexus of the three aging hallmarks, Advanced Glycation End-Product (AGE)/Receptor for AGE emerged as the most statistically robust pathway associated with age-related KOA. This pathway was further supported by analysis of mass spectrometry data. Notably, the change in AGE-RAGE signaling over time was exclusively observed in male mice, suggesting sexual dimorphism in the pathogenesis of age-induced KOA in murine models. Collectively, these findings implicate dysregulation of AGE-RAGE signaling as a sex-dependent driver of age-related KOA.

    DOI: 10.1093/gerona/glab386

    Web of Science

    PubMed

  2. Rate of force development in the quadriceps of individuals with severe knee osteoarthritis: A preliminary cross-sectional study. International journal

    Yusuke Suzuki, Hirotaka Iijima, Masatoshi Nakamura, Tomoki Aoyama

    PloS one   Vol. 17 ( 1 ) page: e0262508   2022

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    Knee osteoarthritis (KOA) is a leading cause of knee pain and disability due to irreversible cartilage degeneration. Previous studies have not identified modifiable risk factors for KOA. In this preliminary cross-sectional study, we aimed to test the following hypotheses: individuals with severe KOA would have a significantly lower quadriceps rate of force development (RFD) than individuals with early KOA, and the decrease in quadriceps RFD would be greater than the decrease in maximum quadriceps strength in individuals with severe KOA. The maximum isometric strength of the quadriceps was assessed in individuals with mild (Kellgren and Lawrence [K&L] grade 1-2) and severe KOA (K&L grade 3-4) using a handheld dynamometer. The RFD was analyzed at 200 ms from torque onset and normalized to the body mass and maximum voluntary isometric contraction torque. To test whether the quadriceps RFD was lowered and whether the lower in the quadriceps RFD was greater than the lower in maximum quadriceps strength in individuals with severe knee OA, the Mann-Whitney U-test and analysis of covariance were performed, respectively. The effect size (ES) based on Hedges' g with a 95% confidence interval (CI) was calculated for the quadriceps RFD and maximum quadriceps strength. Sixty-six participants were analyzed. Individuals with severe KOA displayed significantly lower quadriceps RFD (p = 0.009), the lower being greater than the lower in maximum quadriceps strength (between-group difference, ES: 0.88, -1.07 vs. 0.06, -0.22). Our results suggest that a decreased quadriceps RFD is a modifiable risk factor for progressive KOA. Our finding could help in the early detection and prevention of severe KOA.

    DOI: 10.1371/journal.pone.0262508

    Scopus

    PubMed

  3. Increased recurrent falls experience in older adults with coexisting of sarcopenia and knee osteoarthritis: a cross-sectional study. International journal

    Hirotaka Iijima, Tomoki Aoyama

    BMC geriatrics   Vol. 21 ( 1 ) page: 698 - 698   2021.12

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    BACKGROUND: Sarcopenia and knee osteoarthritis (OA) are two major risk factors for falls in older adults. The coexistence of these two conditions may exacerbate the risk of falls. This cross-sectional study aimed to test the hypothesis that older adults with coexisting sarcopenia and knee OA displayed an increased risk of falls experience. METHODS: Participants recruited from an orthopedic clinic were divided into four groups according to the presence of sarcopenia and radiographic knee OA: isolated sarcopenia, isolated knee OA, sarcopenia + knee OA, and control (i.e., non-sarcopenia with non-OA) groups. We used questionnaires to assess falls experience in the prior 12 months. We performed logistic regression analyses to evaluate the relationship between the four groups and falls experience. RESULTS: Of 291 participants (age: 60-90 years, 78.7% women) included in this study, 25 (8.6%) had sarcopenia + knee OA. Participants with sarcopenia + knee OA had 4.17 times (95% confidence interval: 0.84, 20.6) higher odds of recurrent falls (≥2 falls) than controls after adjustment for age, sex, and body mass index. The increased recurrent falls experience was not clearly confirmed in participants with isolated sarcopenia and isolated knee OA. CONCLUSIONS: People with coexisting of sarcopenia and knee OA displayed increased recurrent falls experience. This study suggests a new concept, "sarcopenic knee OA", as a subgroup associated with higher risk of falls, which should be validated in future large cohort studies. TRIAL REGISTRATION: Not applicable.

    DOI: 10.1186/s12877-021-02654-4

    PubMed

  4. Analysis of Spastic Gait in Patients With Cervical Myelopathy Using the Timed Up and Go Test With a Laser Range Sensor. International journal

    Takafumi Koyama, Koji Fujita, Hirotaka Iijima, Mio Norose, Takuya Ibara, Toru Sasaki, Toshitaka Yoshii, Akimoto Nimura, Masaki Takahashi, Atsushi Okawa

    Spine     2021.11

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    STUDY DESIGN: Cross-sectional study. OBJECTIVE: This study aimed to objectively evaluate spastic gait and reveal its novel characteristics via analysis of gait in patients with cervical myelopathy (CM) using the Timed Up and Go (TUG) test with a laser range sensor. SUMMARY OF BACKGROUND DATA: Among patients with CM, spastic gait is a common diagnostic symptom; thus, objective assessments of spastic gait would be useful for the diagnosis of CM and recognition of disease status. Although spastic gait has been objectively evaluated in previous studies, the methods employed in those studies are not suitable for clinical settings. METHODS: In total, 37 and 24 participants were recruited for a control group and CM group, respectively. CM was diagnosed by spine surgeons. We developed a laser TUG test, in which the position and velocity of both the legs were captured. The parameter values for both groups were statistically compared, and odds ratios were calculated using logistic regression analyses. RESULTS: The total TUG test time, time to stand up, time to first step, number of steps, and trajectory error for the CM group were significantly higher than those for the control group, whereas the average velocity and average stride length for the CM group were significantly lower than those for the control group. There was a significant independent association between the total TUG test time and CM. The optimal cutoff point of the total test time for CM risk was approximately 9 seconds. CONCLUSION: Through the use of the laser TUG test, we were able to identify characteristics of spastic gait, which leads to difficulty in standing and taking the first step, wobbling while walking, and an increased risk of falling. We found that the risk of CM was higher if the individual took longer than 9 seconds to complete the TUG test.Level of Evidence: 4.

    DOI: 10.1097/BRS.0000000000004284

    PubMed

  5. Microcurrent Therapy as a Therapeutic Modality for Musculoskeletal Pain: A Systematic Review Accelerating the Translation From Clinical Trials to Patient Care. International journal

    Hirotaka Iijima, Masaki Takahashi

    Archives of rehabilitation research and clinical translation   Vol. 3 ( 3 ) page: 100145 - 100145   2021.9

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    Objective: To summarize the level of knowledge regarding the effects of microcurrent therapy (MCT) on musculoskeletal pain in adults. Data Sources: The PubMed, Physiotherapy Evidence Database, Cumulative Index to Nursing Allied Health Literature, Cochrane Central Register of Controlled Trials, and Igaku Chuo Zasshi database were searched from the time of their inception to December 2020. Study Selection: Randomized controlled trials (RCTs) investigating the effects of MCT on musculoskeletal pain were included. Additionally, non-RCTs were included to assess the adverse events. Data Extraction: The primary outcomes were pain and adverse events related to MCT. To assess the reproducibility of MCT, we evaluated the completeness of treatment description using the Template for Intervention Description and Replication (TIDieR) checklist. We also assessed the quality of evidence using the Grading of Recommendations Assessment, Development, and Evaluation (GRADE). Data Synthesis: A comprehensive assessment of 4 RCTs and 5 non-RCTs that met the inclusion criteria revealed that MCT significantly improved shoulder pain (1 study, 40 patients) and knee pain (1 study, 52 patients) compared with sham MCT without any severe adverse events. MCT has clinically significant benefits for knee pain. This study also revealed a clinically significant placebo response in treating knee pain. This evidence highlights the substantial effect of placebo response in clinical care. These treatment effects on knee pain are further supported by the high quality of evidence in GRADE with high reproducibility in TIDieR. Conclusions: The findings of this meta-analysis highlight the effect of placebo response in treating knee pain. MCT is a potential, core nonpharmacologic treatment option in clinical care with minimal adverse events and should be further investigated. This study proposes a framework for the future investigation of the effect of MCT on musculoskeletal pain to enhance the study quality and reproducibility.

    DOI: 10.1016/j.arrct.2021.100145

    PubMed

  6. Staircase design for health monitoring in elderly people

    Ami Ogawa, Hirotaka Iijima, Masaki Takahashi

    JOURNAL OF BUILDING ENGINEERING   Vol. 37   2021.5

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:ELSEVIER  

    The need for early detection and intervention for age-related health deterioration via home-based health monitoring systems is growing with global population aging. Health deterioration is typically evaluated using questionnaires, but obtaining frequent responses is difficult. Although stair walking involving a high physical load is suitable for assessing health deterioration, uniform assessment using home-based systems is difficult because different stair designs result in different movements. There is currently insufficient research on differences in evaluation performance between different stair designs. The current study aimed to develop an objective index to assess difficulties in activities of daily living (ADLs) as an indicator of health deterioration by observing stair walking, and to investigate the effects of stair dimensions on the relationship between the index and health deterioration. We hypothesized that knee joint positions during stair ascent would be associated with health deterioration, and developed the "Stepping in Level" index, reflecting the knee joint position when stepping in, obtained using a markerless depth information-based system. Seventy elderly people ascended two types of stairs (steep: 33.7 degrees; shallow: 28.2 degrees) and completed a questionnaire regarding ADL difficulty. We used a generalized linear mixed model to investigate the ability of "Stepping in Level" to explain ADL difficulty. The results revealed that "Stepping in Level" was able to explain ADL difficulty, and steep stairs were more suitable than shallow stairs for assessment. Our system enables daily assessment of difficulty in ADLs and detection of subtle changes in daily life. The current findings could inform design guidelines for health monitoring.

    DOI: 10.1016/j.jobe.2021.102152

    Web of Science

    Scopus

  7. Low Back Pain as a Risk Factor for Recurrent Falls in People With Knee Osteoarthritis. International journal

    Hirotaka Iijima, Kanako Shimoura, Tomoki Aoyama, Masaki Takahashi

    Arthritis care & research   Vol. 73 ( 3 ) page: 328 - 335   2021.3

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    OBJECTIVE: Knee osteoarthritis (OA) has been suggested to increase the risk of falls. Low back pain (LBP) is a potential risk factor for falls in people with knee OA, but this issue has not been addressed adequately in previous studies. The objective of this study was to investigate the relationship between LBP and falls in people with knee OA in a 12-month period. METHODS: Participants with knee OA (Kellgren/Lawrence [K/L] grade ≥1) completed questionnaires for LBP and falls that occurred in the preceding 12 months. Binary and ordinal logistic regression analyses were performed to assess the relationship between LBP or moderate-to-severe LBP (numeric rating scale ≥4 points) and any fall (≥1 fall) or recurrent falls (≥2 falls) after adjustment for age, sex, K/L grade, knee pain severity, and quadriceps strength. Sensitivity analyses were performed excluding people with sciatica, nonchronic LBP, K/L grade 1, and those receiving pain medications. RESULTS: We included 189 participants (ages 61-90 years, 78.3% women) in this study. Of these participants, 41 (21.6%) reported falls in the preceding 12 months. People with any LBP (n = 101) and those with moderate-to-severe LBP (n = 45) had 2.7- and 3.7-times higher odds of recurrent falls, respectively. Sensitivity analyses revealed a strong correlation between moderate-to-severe LBP and recurrent falls. CONCLUSION: Thorough investigation of LBP as a risk factor for recurrent falls in people with knee OA may provide a novel insight into the pathomechanics of recurrent falls in this population.

    DOI: 10.1002/acr.24136

    Web of Science

    Scopus

    PubMed

  8. Relationship Between Varus Thrust During Gait and Low Back Pain in Individuals With Knee Osteoarthritis. International journal

    Hirotaka Iijima, Yusuke Suzuki, Tomoki Aoyama, Masaki Takahashi

    Arthritis care & research   Vol. 72 ( 9 ) page: 1231 - 1238   2020.9

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    OBJECTIVE: To test the hypothesis that varus thrust visualized during gait is associated with a higher prevalence of low back pain (LBP) in individuals with knee osteoarthritis (OA). METHODS: Individuals with knee OA (Kellgren/Lawrence grade ≥1) underwent a gait observation to assess varus thrust. The participants identified LBP and its severity using questionnaires. Logistic regression analyses were performed to examine the association between varus thrust and LBP. RESULTS: We included 205 participants (mean age 68.19 years; 72.20% women). A total of 45 participants (22.0%) showed varus thrust in their painful knee, in whom 31 (68.89%) and 18 (40.00%) were identified as having any LBP and moderate-to-severe LBP (numerical rating scale ≥4 points), respectively. Patients with varus thrust demonstrated a 3.6-fold higher risk of the presence of moderate-to-severe LBP (95% confidence interval [95% CI] 1.62-8.10). In patients with LBP, the presence of varus thrust was associated with more severe LBP intensity (proportional odds ratio 2.25 [95% CI 1.02-4.96]). CONCLUSION: This study highlights the novel relationship between varus thrust and LBP, which supports the idea of a biomechanical link, the so-called knee-spine syndrome. These findings provide new insight for clarifying the pathogenesis of LBP related to knee OA.

    DOI: 10.1002/acr.24020

    Web of Science

    Scopus

    PubMed

  9. Gait analysis of patients with distal radius fracture by using a novel laser Timed Up-and-Go system. International journal

    Koji Fujita, Hirotaka Iijima, Ryo Eguchi, Tomoyuki Kuroiwa, Toru Sasaki, Yukihiro Yokoyama, Takafumi Koyama, Akimoto Nimura, Ryuichi Kato, Atsushi Okawa, Masaki Takahashi

    Gait & posture   Vol. 80   page: 223 - 227   2020.7

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    BACKGROUND: Postmenopausal women are at risk of fall and fracture with the physical decline. Distal radius fracture (DRF) is considered as the primary fragility fracture, and women with this fracture showed poor results in the usual Timed Up-and-Go (TUG) test, indicating a decline in balance and physical ability. The detailed physical characteristics of female DRF patients have not been extensively examined. RESEARCH QUESTION: Is the novel laser TUG system able to detect and analyze the detailed gait characteristics in patients with DRF whose physical ability has tended to decline? METHODS: In this cross-sectional case control study, the gait characteristics of 32 female patients with DRF who had undergone surgery were evaluated at 2 weeks postoperatively with a laser TUG system to analyze the detailed leg motion during normal TUG test. Forty-three age- and sex-matched non-fractured women were evaluated by the laser TUG system as controls. Lifestyle and present illness were corrected at the time of TUG measurement. Detailed data during laser TUG in both groups were compared statistically, and odds ratio and thread shod of the fracture was elucidated through a logistic regression analysis. RESULTS: DRF patients showed slower speed and had to do more steps to complete the TUG test. Furthermore, asymmetric trajectory and significantly further distance from the marker were observed. Thirteen steps to complete the TUG test was the thread shod of DRF. SIGNIFICANCE: Detailed gait characteristics of patients with DRF were detected by the laser TUG system. The gait decline and abnormality could be one of the reasons of consecutive fragility fracture. To prevent secondary fragility fractures, this system can be useful for screening.

    DOI: 10.1016/j.gaitpost.2020.06.005

    PubMed

  10. State of the Field of waist-mounted sensor algorithm for gait events detection: A scoping review. International journal

    Hirotaka Iijima, Masaki Takahashi

    Gait & posture   Vol. 79   page: 152 - 161   2020.6

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    BACKGROUND: A waist-mounted sensor is an attractive option for detecting initial and end of foot contacts during gait in a clinical setting without disturbing the subject's natural gait. RESEARCH QUESTION: To examine the current state of the field regarding waist-mounted sensor algorithms for gait event detection during locomotion in adults. METHODS: A scoping review design was used to search peer-reviewed literature or conference proceedings published through October 2018 for algorithms for gait event detection. We analyzed data from the studies in a descriptive manner. RESULTS: In total, 588 potentially relevant articles were selected, of which 14 (171 participants, mean age: 44.0 years) met the inclusion criteria. We identified 15 algorithms developed using biomechanical theories including the inverted pendulum model that represents gait during level walking. Most algorithms estimated gait events using triaxial acceleration data with an absolute error of approximately 50-100 ms in healthy adults. However, there was a large amount of inter-trial heterogeneity, and only a few algorithms were validated in patients with neurological diseases. Lower gait speed reduced the accuracy of gait event estimation. SIGNIFICANCE: There was no algorithm that showed outstanding performance in the estimation of gait events during level walking using the waist-mounted sensor. More comparisons of all available algorithms with an established reference standard for one data-set are needed to identify the best algorithms. As patients with pathological conditions display altered trunk acceleration and slower gait speeds, the development of an algorithm that does not rely on particular signal characteristics and is robust for a wide range of gait speeds is needed before a specific algorithm can be recommended as a valid strategy for clinical practice.

    DOI: 10.1016/j.gaitpost.2020.03.021

    Web of Science

    Scopus

    PubMed

  11. Transcutaneous Electrical Nerve Stimulation Improves Stair Climbing Capacity in People with Knee Osteoarthritis. International journal

    Hirotaka Iijima, Ryo Eguchi, Kanako Shimoura, Keisuke Yamada, Tomoki Aoyama, Masaki Takahashi

    Scientific reports   Vol. 10 ( 1 ) page: 7294 - 7294   2020.4

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    This study aimed to examine the effect of transcutaneous electrical nerve stimulation (TENS) on stair climbing capacity in individuals with pre-radiographic to mild knee osteoarthritis (OA). This is a secondary analysis of data from a single, participant-blinded, randomized controlled trial with a pre-post design. Participants with pre-radiographic to mild knee OA (mean age, 59.1 years; 72.9% women) were randomly assigned into two groups, a TENS (n = 30) and a sham-TENS groups (n = 29). TENS or sham-TENS treatments were applied to all participants by using the prototype TENS device with pre-specified parameters. The primary outcome measures included valid and reliable functional measures for stair climbing (stair-climb test [SCT]), visual analog scale for knee pain during the SCT, and quadriceps muscle strength. TENS improved SCT time by 0.41 s (95% confidence interval [CI]: 0.07, 0.75). The time reduction in the transition phase explains the TENS therapeutic effect. Post-hoc correlation analyses revealed a non-significant but positive relationship between the pain relief effect and improved 11-step SCT time in the TENS group but not in the sham-TENS group. These results indicate that the TENS intervention may be an option for reducing the burden of early-stage knee OA.

    DOI: 10.1038/s41598-020-64176-0

    Web of Science

    Scopus

    PubMed

  12. Pain catastrophizing affects stair climbing ability in individuals with knee osteoarthritis. International journal

    Yusuke Suzuki, Hirotaka Iijima, Tomoki Aoyama

    Clinical rheumatology   Vol. 39 ( 4 ) page: 1257 - 1264   2020.4

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    OBJECTIVE: The association between pain catastrophizing and ability to perform activities of daily living (ADL) requiring weight bearing, in particular stair climbing, standing from a seated position, and walking, in individuals with knee osteoarthritis (OA) is unclear. This study aimed to investigate the association between pain catastrophizing and ability to perform these ADLs by this population. METHOD: This cross-sectional study included individuals with knee OA (Kellgren and Lawrence grades 1-4). The ability to perform ADL was evaluated using the knee OA-related health domain measure (Japanese Knee Osteoarthritis Measure). Pain catastrophizing was evaluated using the Pain Catastrophizing Scale. The association between pain catastrophizing and ability to perform ADL, in particular stair climbing, standing from a seated position, and walking, was evaluated using the logistic regression analysis. Three types of sensitivity analysis were performed to validate the results of the logistic regression analysis. RESULTS: A total of 151 participants were included in the final analysis. When adjusted for covariates, those with pain catastrophizing showed significantly decreased stair climbing ability (odds ratio 8.84; 95% confidence interval 1.37 to 56.92). Moreover, the results did not change even with sensitivity analysis. By contrast, when adjusted for covariates, those with pain catastrophizing did not show significantly decreased ability to stand from a seated position and walk. CONCLUSION: Pain catastrophizing in knee OA patients showed significantly decreased stair climbing ability. There is a need to consider the effect of pain catastrophizing in rehabilitation of knee OA patients with decreased ability to climb stairs.Key Points• Pain catastrophizing in knee OA patients showed significantly decreased stair climbing ability.• Ability to stand from a seated position and walk were not affected by pain catastrophizing.• There is a need to consider the effect of pain catastrophizing in rehabilitation of knee OA patients with decreased ability to climb stairs.

    DOI: 10.1007/s10067-019-04881-y

    PubMed

  13. Hip abductor muscle weakness and slowed turning motion in people with knee osteoarthritis. International journal

    Hirotaka Iijima, Ayanori Yorozu, Yusuke Suzuki, Ryo Eguchi, Tomoki Aoyama, Masaki Takahashi

    Journal of biomechanics   Vol. 101   page: 109652 - 109652   2020.3

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    Laser range sensor-based timed up and go (laser-TUG) test can evaluate performance in TUG subtasks (sit-to-walk [STW], walking a short distance, and turning). This study aimed to test the hypothesis that weaker hip abductor muscle strength is more significantly associated with slowed turning speed than with the other TUG subtasks (STW and straight walking) after controlling for quadriceps muscle strength in patients with knee osteoarthritis (OA). Community-dwelling participants with knee OA (Kellgren and Lawrence [K&L] grade ≥ 1; mean age, 68.6 years; 70.3% women) underwent laser-TUG. Spatiotemporal gait parameters in TUG and the TUG subtasks were evaluated as outcome measures. The isometric muscle strength of the hip abductor and quadriceps was measured using a hand-held dynamometer. Multiple linear regression analysis was performed to examine the relationship between muscle strength as an independent variable and spatiotemporal parameters as dependent variables. The relative importance of hip abductor muscle strength was determined using the percentages of unique variance. Participants with weaker hip abductor muscle strength demonstrated 0.094 m/s slower turning speed after adjustment for covariates including quadriceps muscle strength. The unique variance explained by hip abductor muscle strength in turning speed was 2.1%. However, no significant relationships were confirmed between weak hip abductor muscle strength and the time to perform TUG and the straight walking (forward and return) phase. These findings indicate that turning motion may be more sensitive to aggravated hip abductor muscle weakness and may show better response to hip muscle strengthening exercises. Longitudinal studies are warranted to elucidate this issue.

    DOI: 10.1016/j.jbiomech.2020.109652

    PubMed

  14. Contralateral limb effect on gait asymmetry and ipsilateral pain in a patient with knee osteoarthritis: A proof-of-concept case report Reviewed

    Hirotaka Iijima, Mami Inoue, Yusuke Suzuki, Kanako Shimoura, Tomoki Aoyama, Katsuyuki Madoba, Masaki Takahashi

    JBJS Case Connect   Vol. 10 ( 1 )   2020

     More details

    Authorship:Lead author  

  15. Detailed analysis of the transverse arch of hallux valgus feet with and without pain using weightbearing ultrasound imaging and precise force sensors. International journal

    Hala Zeidan, Eguchi Ryo, Yusuke Suzuki, Hirotaka Iijima, Yuu Kajiwara, Keiko Harada, Kengo Nakai, Kanako Shimoura, Koji Fujimoto, Masaki Takahashi, Tomoki Aoyama

    PloS one   Vol. 15 ( 1 ) page: e0226914   2020

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    BACKGROUND: Hallux valgus is the most common forefoot deformity and affects the transverse arch structure and its force loading patterns. This study aims to clarify the differences in the transverse arch structure and the force under the metatarsal heads individually, between normal feet and hallux valgus feet, and between hallux valgus feet with pain and without pain. We further test the association between the parameters of the transverse arch and hallux valgus angle and between the parameters and pain in hallux valgus. METHODS: Women's feet (105 feet) were divided into normal group (NORM) and hallux valgus group (HVG); and further into subgroups: hallux valgus without pain (HV Pain (-)) and hallux valgus with pain (HV Pain (+)). Transverse arch height and metatarsal heads height were measured using weight-bearing ultrasound imaging. Force under the metatarsal heads was measured using force sensors attached directly on the skin surface of the metatarsal heads. The measurements were taken in three loading positions: sitting, quiet standing and 90% weight shift on the tested foot. Differences between the groups were compared using Student t-test and Wilcoxon Exact test. Multivariate logistic analysis with adjustment for physical characteristics was also conducted. RESULTS: Transverse arch height was significantly higher in HVG than in NORM in all positions; there were no significant differences between HV Pain (+) and HV pain (-). Lateral sesamoid was significantly higher in HVG and HV Pain (+) than in NORM and HV Pain (-) respectively when bearing 90% of the body weight unilaterally. There was a trend of higher forces under the medial forefoot without significant difference. Transverse arch height and lateral sesamoid height were associated with the hallux valgus angle, while lateral sesamoid height was associated with forefoot pain in hallux valgus deformity. CONCLUSIONS: This study shows the differences in the transverse arch structure between normal feet and feet with hallux valgus, and between hallux valgus feet with and without pain. This finding is noteworthy when considering future treatments of painful feet, notably the height of the lateral sesamoid which seems to play a role in forefoot pain.

    DOI: 10.1371/journal.pone.0226914

    PubMed

  16. Altered sagittal plane kinematics and kinetics during sit-to-stand in individuals with knee osteoarthritis: A systematic review and meta-analysis. International journal

    Moeka Sonoo, Hirotaka Iijima, Naohiko Kanemura

    Journal of biomechanics   Vol. 96   page: 109331 - 109331   2019.11

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    Knee osteoarthritis (OA) progression is associated with an increase in peak external knee flexion torque (EKFT) during gait. However, the difference in the peak EKFT between individuals with knee OA and age-matched subjects was unclear. Therefore, to understand the kinematics, kinetics, and electromyogram characteristics in individuals with knee OA, we conducted a systematic review and meta-analysis of sagittal plane-dominant sit-to-stand (STS) motion. PubMed, PEDro, CINAHL, and Cochrane CENTRAL were used. Fourteen articles (knee-OA group: n = 323, mean age = 65.0 years, mean weight = 79.2 kg; control group: n = 224, mean age = 64.4 years, mean weight = 70.4 kg) were included, and the pooled standardized mean differences (SMDs) were calculated using a random-effects model. Meta-analysis showed that individuals with knee OA present significantly lower peak EKFT (pooled SMD: -1.62; 95% confidence interval [CI]: -2.36 to -0.88), significantly large trunk flexion angle (pooled SMD: 1.04; 95% CI: 0.69 to 1.39), and no significantly lower peak external knee adduction torque despite the significantly larger lateral-lean angle to the less affected side (pooled SMD: 1.04; 95% CI: 0.69 to 1.39). The quality of evidence for all outcomes was very low. The descriptive synthesis indicates that STS motion in individuals with knee OA might be an unsuitable motion strategy for knee muscle activity, force utilization from multiple joints to the knee joint, and their influence on knee joint cartilage, despite their lower peak EKFT. This information can help to better understand movement strategies and to optimize treatment approaches for individuals with knee OA.

    DOI: 10.1016/j.jbiomech.2019.109331

    PubMed

  17. Lower-body positive pressure diminishes surface blood flow reactivity during treadmill walking. International journal

    Junichi Tajino, Akira Ito, Yusuke Torii, Koji Tsuchimoto, Hirotaka Iijima, Xiangkai Zhang, Momoko Tanima, Shoki Yamaguchi, Hiroshi Ieki, Ryosuke Kakinoki, Hiroshi Kuroki

    BMC research notes   Vol. 12 ( 1 ) page: 733 - 733   2019.11

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    OBJECTIVE: The purpose of this study was to determine the effects of the lower-body positive pressure on surface blood flow during standing still and treadmill walking to explore cardiovascular safety for application to rehabilitation treatment. Thirteen healthy volunteers participated in the experiment and surface blood flows were measured in the forehead, thigh, calf, and the top of the foot during standing still and walking under various pressure conditions (0 kPa, 5 kPa, and 6.7 kPa). RESULTS: Lower-body positive pressure decreased the blood flow in the forehead and the thigh during walking (p < .05 for each), whereas an increasing trend in blood flow was observed during standing still (p < .05). Furthermore, in the forehead and thigh, the extent of blood flow increase at the onset of walking was found to decrease in accordance with the applied pressure (p < .01 for each). These findings suggest that during walking, lower-body positive pressure modulates the blood flow, which implies safeness of this novel apparatus for use during orthopedic rehabilitation treatment.

    DOI: 10.1186/s13104-019-4766-2

    Web of Science

    Scopus

    PubMed

  18. Patients with early-stage knee osteoarthritis and knee pain have decreased hip abductor muscle strength while descending stairs. International journal

    Yusuke Suzuki, Hirotaka Iijima, Kanako Shimoura, Tadao Tsuboyama, Tomoki Aoyama

    Clinical rheumatology   Vol. 38 ( 8 ) page: 2249 - 2254   2019.8

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    INTRODUCTION/OBJECTIVES: This study aimed to investigate the association between hip abductor muscle strength and knee pain in patients with early-stage knee osteoarthritis (OA) while ascending and descending stairs. METHOD: This cross-sectional study included individuals with early-stage knee OA (Kellgren/Lawrence grades 1 or 2). Knee pain while ascending and descending stairs was evaluated using a knee OA-related health domain measure (Japanese Knee Osteoarthritis Measure). Knee extension and hip abductor muscle strength were also evaluated. The association between hip abductor muscle strength and knee pain while ascending and descending stairs was evaluated using multiple regression analysis. RESULTS: A total of 157 participants were included in the final analysis. After the adjustment for age, sex, and knee extension strength, those with knee pain while descending stairs showed significantly decreased hip abductor muscle strength (β, - 0.09; 95% confidence interval [CI], - 0.19 to - 0.003). In contrast, after the adjustment for age, sex, and knee extension muscle strength, those with knee pain while ascending stairs did not have significantly decreased hip abductor muscle strength (β, - 0.06; 95% CI, - 0.15 to 0.02). CONCLUSIONS: Patients with early-stage knee OA and knee pain while descending stairs had significantly reduced hip abductor muscle strength.

    DOI: 10.1007/s10067-019-04523-3

    Web of Science

    Scopus

    PubMed

  19. Stair climbing ability in patients with early knee osteoarthritis: Defining the clinical hallmarks of early disease. International journal

    Hirotaka Iijima, Ryo Eguchi, Kanako Shimoura, Tomoki Aoyama, Masaki Takahashi

    Gait & posture   Vol. 72   page: 148 - 153   2019.7

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    BACKGROUND: A growing clinical interest has been shown towards identifying knee osteoarthritis (OA) patients at earlier stages. The early detection of knee OA may allow for more effective interventions. RESEARCH QUESTION: The aim of this study was to determine the discriminative ability of a stair-climb test (SCT) in identifying patients with early knee OA, and to determine if descending stair time during the SCT is better than ascending stair time for the identification of these patients. METHODS: This study was a secondary, cross-sectional analysis of baseline data from a randomized controlled trial. Adults with moderate to severe knee pain were enrolled (n = 57; mean age 58.9 years; 71.9% women). Each participant performed an 11-step SCT (11-SCT) while wearing shoes with a pressure sensor insole. A receiver operating characteristic analysis was used to examine the discriminative power of 11-SCT for identifying early knee OA (Kellgren and Lawrence grade 1). The discriminative power was also compared between the ascending and descending 11-SCT time as evaluated by the pressure sensor. RESULTS: The 11-SCT time in patients with early knee OA was 0.55 s longer than that in those with symptomatic non-radiographic OA. A one-second increase in the 11-SCT time was significantly associated with 1.9-fold increased odds of early knee OA being present. The 11-SCT value with the best balance of sensitivity and specificity for identifying early knee OA was 8.33 s (area under the curve: 0.711). The descending time was not significantly better than the ascending time for identifying early knee OA. SIGNIFICANCE: This study determined the time values of an 11-SCT that may be useful for identifying early knee OA patients. These preliminary findings may serve as the foundation for future studies investigating the clinical hallmarks associated with early knee OA.

    DOI: 10.1016/j.gaitpost.2019.06.004

    Web of Science

    Scopus

    PubMed

  20. Effects of interaction between varus thrust and ambulatory physical activity on knee pain in individuals with knee osteoarthritis: an exploratory study with 12-month follow-up. International journal

    Hirotaka Iijima, Tomoki Aoyama, Ryo Eguchi, Masaki Takahashi, Shuichi Matsuda

    Clinical rheumatology   Vol. 38 ( 6 ) page: 1721 - 1729   2019.6

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    INTRODUCTION: This study aimed to examine the interaction effect between ambulatory physical activity (PA) and varus thrust on knee pain in individuals with knee osteoarthritis (OA). METHOD: Subjects (n = 207; mean age: 73.1 years, 71.5% women) in orthopedic clinics with diagnosed knee OA (Kellgren/Lawrence grade ≥ 1) were enrolled in this 12-month observational cohort study. Participants underwent gait observation for varus thrust assessment and pedometer-based ambulatory PA measurements at baseline and 12-month follow-up. Knee pain intensity was assessed using the Japanese Knee Osteoarthritis Measure pain subscale as a primary outcome measure. Multiple linear regression analyses were performed to evaluate ambulatory PA-thrust interaction on knee pain intensity. RESULTS: Ninety-two subjects (mean age, 73.4 years; 68.5% women) completed the 12-month follow-up assessment. Baseline ambulatory PA-thrust interaction was significant (P = 0.017) in the cross-sectional analysis, adjusting for covariates, which yielded R2 = 0.310. Subgroup analysis showed that varus thrust was significantly associated with worse knee pain in subjects walking ≥ 5000 steps/day adjusting for covariates (beta: 7.94; 95% CI: 3.82, 12.1; P < 0.001) with a higher predictive ability (R2 = 0.664). In contrast, ambulatory PA-thrust interaction in the longitudinal analysis showed no significant association with knee pain changes. CONCLUSIONS: Ambulatory PA interacted with varus thrust in the association with knee pain, as coexisting high ambulatory PA and varus thrust had the strongest association with higher knee pain. Maximal pain relief effects might be achieved when both ambulatory PA and varus thrust are treated simultaneously, rather than treating each separately.

    DOI: 10.1007/s10067-019-04472-x

    PubMed

  21. Proximal gait adaptations in individuals with knee osteoarthritis: A systematic review and meta-analysis. International journal

    Hirotaka Iijima, Kanako Shimoura, Tomoko Ono, Tomoki Aoyama, Masaki Takahashi

    Journal of biomechanics   Vol. 87   page: 127 - 141   2019.4

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    Clarifying proximal gait adaptations as a strategy to reduce knee joint loading and pain for individuals with knee osteoarthritis (OA) contributes to understanding the pathogenesis of multi-articular OA changes and musculoskeletal pain in other joints. We aimed to determine whether biomechanical alterations in knee OA patients during level walking is increased upper trunk lean in the frontal and sagittal planes, and subsequent alteration in external hip adduction moment (EHAM) and external hip flexion moment (EHFM). A literature search was conducted in PubMed, PEDro, CINAHL, and Cochrane CENTRAL through May 2018. Where possible, data were combined into a meta-analysis; pooled standardized mean differences (SMD) of between knee OA patients and healthy adults were calculated using a random-effect model. In total, 32 articles (2037 participants, mean age, 63.0 years) met inclusion criteria. Individuals with knee OA had significantly increased lateral trunk lean toward the ipsilateral limb (pooled SMD: 1.18; 95% CI: 0.59, 1.77) along with significantly decreased EHAM. These subjects also displayed a non-significantly increased trunk/pelvic flexion angle and EHFM. The GRADE approach judged all measures as "very low." These results may indicate that biomechanical alterations accompanying knee OA are associated with increased lateral trunk lean and ensuing alterations in EHAM. Biomechanical alterations in the sagittal plane were not evident. Biomechanical adaptations might have negative sequelae, such as secondary hip abductor muscle weakness and low back pain. Thus, investigations of negative sequelae due to proximal gait adaptations are warranted.

    DOI: 10.1016/j.jbiomech.2019.02.027

    Web of Science

    Scopus

    PubMed

  22. Quadriceps Weakness in Individuals with Coexisting Medial and Lateral Osteoarthritis. International journal

    Hirotaka Iijima, Yusuke Suzuki, Tomoki Aoyama, Masaki Takahashi

    JB & JS open access   Vol. 4 ( 1 ) page: e0028   2019.3

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    Background: This study examined whether individuals who have mild medial osteoarthritis (OA) of the knee with coexisting lateral OA have less muscle strength than individuals who do not have lateral OA. Methods: A series of 153 individuals (84% of whom were women) between 48 and 88 years old who had Kellgren and Lawrence (KL) grade-2 OA in the medial compartment of the knee underwent radiographic evaluation to assess the presence of lateral OA, which was graded with the system of the Osteoarthritis Research Society International (OARSI) atlas as well as the KL system. The isometric maximum strengths of the quadriceps, the hip abductors, and the hip extensors were evaluated with use of a handheld dynamometer. Results: Individuals who had coexisting medial and lateral OA had more severe knee pain and weaker quadriceps than those who did not have lateral OA. The study adjusted for age and sex both for the OARSI atlas system (adjusted difference in mean strength: 0.272 Nm/kg, 95% confidence interval [CI]: 0.143 to 0.401 Nm/kg) and for KL grading (adjusted difference in mean strength: 0.185 Nm/kg, 95% CI: 0.061 to 0.309 Nm/kg). Logistic regression analysis showed that weakness of the quadriceps increased the odds of the presence of lateral OA sevenfold after adjustments using the OARSI atlas were made for age, sex, anatomical axis, range of motion of the knee, and intensity of pain in the knee. Conclusions: Individuals who had coexisting medial and lateral OA had weaker quadriceps than individuals who had mild medial OA alone. Paying close attention to quadriceps weakness might provide a key to clarifying the pathogenesis of bicompartmental disease in the tibiofemoral joint. Level of Evidence: Prognostic Level III. See Instructions for Authors for a complete description of levels of evidence.

    DOI: 10.2106/JBJS.OA.18.00028

    Scopus

    PubMed

  23. Trunk movement asymmetry associated with pain, disability, and quadriceps strength asymmetry in individuals with knee osteoarthritis: a cross-sectional study. International journal

    H Iijima, R Eguchi, T Aoyama, M Takahashi

    Osteoarthritis and cartilage   Vol. 27 ( 2 ) page: 248 - 256   2019.2

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    OBJECTIVE: This study examined 1) the clinical relevance of trunk movement asymmetry, which was evaluated using a trunk-mounted inertial measurement unit (IMU), and 2) the relationship between trunk movement asymmetry and lower limb muscle strength asymmetry in individuals with knee osteoarthritis (OA). DESIGN: One-hundred-thirty-one participants (mean age, 74.2 years; 71.8% female; Kellgren and Lawrence [K&L] grade ≥1) underwent gait analysis at their preferred pace for IMU-based measurement of trunk movement asymmetry (harmonic ratio [HR] and improved HR). The isometric strength of quadriceps and hip abductors was evaluated using a hand-held dynamometer. Pain and disability level were evaluated using a validated self-reported questionnaire. Multiple regression analyses with covariate adjustment were performed to examine the relationship between trunk movement asymmetry (independent variable) and pain, disability level, or muscle strength asymmetry (dependent variables). RESULTS: Individuals with severe knee OA (K&L grade ≥3) had increased trunk movement asymmetry in the medio-lateral axis compared to those with a K&L grade of 1. Increased trunk movement asymmetry was associated with a greater knee pain and disability. The increased trunk movement asymmetry was significantly associated with an increase in the asymmetry of quadriceps strength, but not with asymmetry in the strength of hip abductor. CONCLUSION: Our findings indicate that increased medio-lateral trunk movement asymmetry may be an indicator of impairment, rather than adaptation, in individuals with knee OA. This preliminary finding warrants validation by future study. Paying close attention to medio-lateral trunk movement asymmetry may be key to our understanding of OA-related pain and disability.

    DOI: 10.1016/j.joca.2018.10.012

    Web of Science

    Scopus

    PubMed

  24. Concurrent validity and measurement error of stair climb test in people with pre-radiographic to mild knee osteoarthritis. International journal

    Hirotaka Iijima, Kanako Shimoura, Ryo Eguchi, Tomoki Aoyama, Masaki Takahashi

    Gait & posture   Vol. 68   page: 335 - 339   2019.2

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    BACKGROUND: Stair climbing is the task first affected in patients with knee osteoarthritis (OA); therefore, the precise measurement of time required to climb stairs is important to identify mobility limitations, particularly in the early phase of knee OA. RESEARCH QUESTION: This study aimed to examine the test-retest reliability, measurement error, and concurrent validity of the stopwatch-based stair-climb test (SCT) in adults with pre-radiographic to mild knee OA. METHODS: Fifty-nine participants (mean age, 59.1 [range, 50-69] years; 72.9% female) with Kellgren and Lawrence grade ≤2 disease underwent an 11-step SCT (11-SCT) in accordance with the Osteoarthritis Research Society International recommended method while wearing pressure sensor-mounted standard shoes that is used as a gold standard procedure. Test-retest reliability, measurement errors, and the concurrent validity of the stopwatch-based 11-SCT were evaluated. RESULTS: The test-retest reliability of the stopwatch-based 11-SCT was excellent (intra-class correlation coefficient1,1 [ICC1,1], 0.952; 95% confidence interval [CI], 0.560 to 0.985; p <  0.001) and the minimal detectable change95 was 0.102 s. Concurrent validity was excellent (ICC2,1: 0.957; 95% CI: 0.661 to 0.986; p <  0.001). SIGNIFICANCE: The stopwatch-based 11-SCT had high test-retest reliability and high concurrent validity, which justify its clinical use for identifying mobility limitations in individuals with pre-radiographic to mild knee OA. A difference of 0.2 s in the stopwatch-based 11-SCT time would be considered a true difference beyond a 95% measurement error.

    DOI: 10.1016/j.gaitpost.2018.12.014

    PubMed

  25. Immediate Effects of Transcutaneous Electrical Nerve Stimulation on Pain and Physical Performance in Individuals With Preradiographic Knee Osteoarthritis: A Randomized Controlled Trial. International journal

    Kanako Shimoura, Hirotaka Iijima, Yusuke Suzuki, Tomoki Aoyama

    Archives of physical medicine and rehabilitation   Vol. 100 ( 2 ) page: 300 - 306   2019.2

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    OBJECTIVE: To investigate the effect of transcutaneous electrical nerve stimulation (TENS) on knee pain and comprehensive physical function in preradiographic knee osteoarthritis. DESIGN: A single, participant-blinded, randomized controlled trial (RCT) with pre-post design. SETTING: University research laboratory. PARTICIPANTS: Patients with knee pain belonging to Kellgren-Lawrence grade 0 or 1 (N=50; aged 50-69y) were randomly assigned to the TENS (n=25) and sham-TENS groups (n=25). INTERVENTIONS: All participants wore the TENS device under the patella of the symptomatic knee. After measurement, the TENS devices in the TENS group were turned on, and those in the sham-TENS group were not connected. MAIN OUTCOME MEASURES: The primary outcome measures included the stair climb test, timed Up and Go (TUG) test, 6-minute walk test (6MWT), and knee pain evaluated using the visual analog scale (VAS) for stair climb test, TUG test, and 6MWT. Secondary outcomes included knee extensor strengths and the 2-step test and stand-up test from the locomotive syndrome risk test. RESULTS: Multiple regression analysis revealed that TENS intervention significantly improved the walk distance and VAS score of the 6MWT, after adjusting for premeasurement data (distance; P=.015, VAS; P=.030). CONCLUSIONS: Use of TENS improved the VAS score for pain and the distance walked in the 6MWT for individuals with Kellgren-Lawrence grade 0 or 1 of the knee. Thus, TENS may be effective for long-distance walking in patients with preradiographic knee osteoarthritis.

    DOI: 10.1016/j.apmr.2018.08.189

    PubMed

  26. The Association of Variations in Hip and Pelvic Geometry With Pregnancy-Related Sacroiliac Joint Pain Based on a Longitudinal Analysis. International journal

    Xiang Ji, Saori Morino, Hirotaka Iijima, Mika Ishihara, Mirei Kawagoe, Fumiko Umezaki, Yoko Hatanaka, Mamoru Yamashita, Tadao Tsuboyama, Tomoki Aoyama

    Spine   Vol. 44 ( 2 ) page: E67-E73 - E73   2019.1

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    STUDY DESIGN: Cross-sectional study using radiological measurements and longitudinal data analysis. OBJECTIVE: We aim to explore hip/pelvic geometry on anteroposterior radiographs and examine if such parameters are associated with clinical symptoms. SUMMARY OF BACKGROUND DATA: Pregnancy-related sacroiliac joint pain is a common disease and is responsible to the disability of daily activities. The etiology is likely to be correlated with the biomechanical factors which are determined by trunk load and hip/pelvic geometry. Previous studies have already found the association between symptoms and weight increase during pregnancy. However, the relationship between bony anatomy and pregnancy-related sacroiliac joint pain remains unknown. METHODS: In total, 72 women were included in the final analysis. In pregnant women with self-reported sacroiliac joint pain, pain scores at 12, 24, 30, and 36 weeks of pregnancy were recorded and included in a mixed-effect linear regression model as dependent variables. The radiological measurements were included as independent variables. Furthermore, to investigate the relationship between hip/pelvic geometry and the activity-specific nociceptive phenomenon, the radiological measurements between patients with and without activity-induced pain were compared using a binominal logistic regression model. RESULTS: The relative bilateral is chial tuberosity distance (betta coefficient: 0.078; P = 0.015) and the relative bilateral femoral head length (betta coefficient: 0.011; P = 0.028) showed significant interactions with the slope of pain scores. Moreover, women whose pain exacerbate during prolonged walking had a higher odds in hip/pelvic geometry of the bilateral ischial tuberosity distance (odds ratio [OR]: 1.12; P = 0.050) and the bilateral femoral head length (OR: 1.16; P = 0.076) with approximately significant P-value. CONCLUSION: These data indicate hip/pelvic anatomical variations are associated with the degree of pain increasing and the activity-specific pain during pregnancy, which may help to have further understanding on the biomechanical factor in developing pregnancy-related sacroiliac joint pain. LEVEL OF EVIDENCE: 3.

    DOI: 10.1097/BRS.0000000000002774

    Web of Science

    Scopus

    PubMed

  27. Periodic mild heat stimuli diminish extracellular matrix synthesis in pellet cultured human chondrocytes. International journal

    Akira Ito, Tomoki Aoyama, Hirotaka Iijima, Kohei Nishitani, Junichi Tajino, Hiroshi Kuroki

    BMC research notes   Vol. 12 ( 1 ) page: 16 - 16   2019.1

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    OBJECTIVE: This study aimed to clarify the effects of periodic mild heat stimuli on extracellular matrix (ECM) synthesis of adult human chondrocytes in 3-dimensional pellet culture. RESULTS: Human articular chondrocytes were subjected to pellet culture at 37 °C for 3 days. Thereafter, the pellets were divided into three groups: 32 °C group which was cultured at 32 °C without heat stimuli, 32 °C + Heat group which was cultured at 32 °C and applied periodic heat stimuli, 37 °C group which was cultured at 37 °C. Heat stimuli were given by transferring the pellets into a CO2 incubator set at 41 °C for 20 min/day, 6 times/week. ECM synthesis ability was evaluated by analyzing the mRNA expressions. Additionally, the collagen and proteoglycan content in the pellet was quantified. DNA content was also measured for estimating the cell amount. We found that there were no significant differences in the mRNA expression of COL2A1, COL1A1, and ACAN between the 32 °C group and 32 °C + Heat group. However, the collagen content per cell and DNA content were significantly lower in the 32 °C + Heat group compared to other groups. Our results indicate that periodic mild heat stimuli may diminish ECM synthesis due to inhibition of collagen production and loss of cells.

    DOI: 10.1186/s13104-019-4058-x

    PubMed

  28. Home exercise therapy to improve muscle strength and joint flexibility effectively treats pre-radiographic knee OA in community-dwelling elderly: a randomized controlled trial. International journal

    Yusuke Suzuki, Hirotaka Iijima, Yuto Tashiro, Yuu Kajiwara, Hala Zeidan, Kanako Shimoura, Yuichi Nishida, Tsubasa Bito, Kengo Nakai, Masataka Tatsumi, Soyoka Yoshimi, Tadao Tsuboyama, Tomoki Aoyama

    Clinical rheumatology   Vol. 38 ( 1 ) page: 133 - 141   2019.1

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    To compare the efficacy and adherence rates of two parallel home exercise therapy programs-multiple exercise (training and stretching the knee and hip muscles) and control (training the quadriceps muscles)-on knee pain, physical function, and knee extension strength in community-dwelling elderly individuals with pre-radiographic knee osteoarthritis (OA). One hundred patients with medial knee pain were randomly allocated to one of two 4-week home exercise programs. Individuals with a Kellgren/Lawrence (K/L) grade 0 or 1 OA (pre-radiographic knee OA) in the medial compartment were enrolled. Primary outcomes were knee pain (visual analog scale), self-reported physical function (Japanese Knee Osteoarthritis Measure [JKOM]), and isometric maximum muscle strength of the knee extensor measured using a hand-held dynamometer. A total of 52 patients (28 [53.8%] in the multiple exercise group, 24 [46.2%] in the control group) completed the trial. The JKOM activities of daily living and general health conditions outcomes improved significantly in the multiple exercise group compared to the control group (JKOM activities of daily living, beta = - 0.76; 95% confidence interval [CI], - 1.39 to - 0.13; p = 0.01; JKOM general health conditions, beta = - 0.25; 95% CI, - 0.48 to - 0.01; p = 0.03). The home exercise compliance rates of the multiple exercise and control groups were 96.6 and 100%, respectively. When targeting pre-radiographic knee OA in community-dwelling elderly, it is important to implement home exercise programs that aim to improve muscle strength and joint flexibility rather than knee extension muscle power only.

    DOI: 10.1007/s10067-018-4263-3

    PubMed

  29. Development of a questionnaire survey to evaluate lower limb function of patients with knee osteoarthritis. International journal

    Yusuke Suzuki, Hirotaka Iijima, Yuto Tashiro, Yuu Kajiwara, Hala Zeidan, Kanako Shimoura, Yuichi Nishida, Tsubasa Bito, Kengo Nakai, Masataka Tatsumi, Soyoka Yoshimi, Tadao Tsuboyama, Tomoki Aoyama

    Clinical rheumatology   Vol. 37 ( 11 ) page: 3115 - 3123   2018.11

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    This study aimed to develop a self-questionnaire index relevant to lower limb functional assessment reflecting clinical symptoms and activity restrictions associated with activities of daily living (ADL) for patients with knee osteoarthritis. This cross-sectional study enrolled patients with knee osteoarthritis (Kellgren/Lawrence grade ≥ 1). Participants evaluated clinical symptoms and activity restrictions using self-reported questionnaires. Radiographic evaluation, knee joint range of motion (ROM), and lower limb muscle strength were assessed. Associations between clinical symptoms, activity restrictions, and knee and hip joint function were evaluated using multiple and logistic regression analysis. Overall, 142 participants were included in the final analysis. Patients with knee pain while rising from a chair had lower knee and hip extension strength (knee extension β = - 0.28; 95% confidence interval (CI), - 0.41 to - 0.14; P < 0.0001; hip extension β = - 0.26; 95% CI, - 0.42 to - 0.08; P = 0.0034), and restricted knee extension ROM (odds ratio [OR] = 2.17; 95% CI, 1.02-4.63; P = 0.041). Patients with knee pain during stair climbing, turning or who were unable to stand on one leg without external support had reduced hip abduction muscle strength (β = - 0.17; 95% CI, - 0.27 to - 0.07; P = 0.0008). Patients with knee pain while rising from a chair or stepping showed more severe knee joint degeneration on radiographic evaluation (OR = 3.26; 95% CI, 1.11-10.91; P = 0.03). The self-questionnaire index reflected clinical symptoms and activity restrictions associated with ADL and was significantly associated with lower limb function in individuals with knee osteoarthritis.

    DOI: 10.1007/s10067-018-4229-5

    PubMed

  30. Ultrasound Parameters for Human Osteoarthritic Subchondral Bone ex Vivo: Comparison with Micro-Computed Tomography Parameters. International journal

    Wataru Kiyan, Yasuaki Nakagawa, Akira Ito, Hirotaka Iijima, Kohei Nishitani, Momoko Tanima-Nagai, Shogo Mukai, Junichi Tajino, Shoki Yamaguchi, Akihiro Nakahata, Jue Zhang, Tomoki Aoyama, Hiroshi Kuroki

    Ultrasound in medicine & biology   Vol. 44 ( 10 ) page: 2115 - 2130   2018.10

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    The aim of this study was to identify ultrasound parameters reflecting subchondral porosity (Po), subchondral plate thickness (Tpl) and bone volume fraction at the trabecular bone region (BV/TVTb). Sixteen osteoarthritic human lateral femoral condyles were evaluated ex vivo using a 15-MHz pulsed-echo ultrasound 3-D scanning system. The cartilage-subchondral bone (C-B) surface region (layer 1) and inner subchondral bone region (layer 2) were analyzed; we newly introduced entropy (ENT) and correlation (COR) of ultrasound texture parameters of the parallel (x) or perpendicular (z) direction to the C-B interface for this analysis. Po, Tpl and BV/TVTb were evaluated as reference measurements using micro-computed tomography. ENTL1x (ENT of layer 1, x-direction) and ENTL1z were significantly correlated with Po (both r values = 0.58), CORL2x with Tpl (r = -0.73) and CORL2z with BV/TVTb (r = -0.66). These are efficient indicators of the characteristics of osteoarthritis-related subchondral bone; the other texture parameters were not significant.

    DOI: 10.1016/j.ultrasmedbio.2018.06.003

    Web of Science

    Scopus

    PubMed

  31. Interaction between low back pain and knee pain contributes to disability level in individuals with knee osteoarthritis: a cross-sectional study. International journal

    H Iijima, Y Suzuki, T Aoyama, M Takahashi

    Osteoarthritis and cartilage   Vol. 26 ( 10 ) page: 1319 - 1325   2018.10

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    OBJECTIVE: To test the hypothesis that the interaction between low back pain (LBP) and knee pain intensity contributes to the disability level of individuals with knee osteoarthritis (OA). DESIGN: Community-dwelling participants with knee OA (Kellgren/Lawrence [K/L] grade ≥1) were enrolled. LBP and its severity were identified using questionnaires. Knee pain severity and disability level were evaluated using the Japanese Knee Osteoarthritis Measure (JKOM) subscale. Multiple linear regression analyses were performed to examine the effect of the LBP-knee pain interaction, an independent variable, on disability, a dependent variable. RESULTS: A total of 260 participants (age, 48-88 years; 77.7% women) were included. Of them, 151 (58.1%) had LBP. The LBP-knee pain interaction was significantly associated with disability after the adjustment for covariates. A post-hoc subgroup analysis revealed that the relationship between knee pain intensity and disability level was higher in individuals with LBP (beta: 0.621 points; 95% confidence interval [CI]: 0.511 to 0.731 points) than in those without LBP (beta: 0.402 points; 95% CI: 0.316 to 0.487 points). CONCLUSIONS: LBP interacts with knee pain intensity and contributes to disability level in individuals with knee OA. Coexisting LBP and knee pain had a stronger impact on disability level than LBP or knee pain alone. These findings highlight the potential deteriorative effects of the LBP-knee interaction on disability. Maximal treatment effects for disability might be achieved when LBP and knee pain are targeted simultaneously, rather than separately.

    DOI: 10.1016/j.joca.2018.06.012

    PubMed

  32. Effects of Kilohertz-frequency Electrical Stimulation on Muscle Performance and Adverse Event in Individuals with Neuromuscular Skeletal Disorders:A Systematic Review

    Iijima Hirotaka, Takahashi Masaki, Tashiro Yuto, Aoyama Tomoki

    The Japanese Journal of Rehabilitation Medicine   Vol. 55 ( 9 ) page: 784 - 790   2018.9

     More details

    Language:Japanese   Publisher:The Japanese Association of Rehabilitation Medicine  

    <p>Objective:This study aimed to examine the effects of kilohertz-frequency alternating current (KFAC) on muscle performance and adverse event in patients with neuromuscular skeletal disorders.</p><p>Methods:A literature search was conducted in PubMed, PEDro, CINAHL, and Cochrane CENTRAL until September 2017. The reviewer extracted data on subject characteristics, intervention, target muscle, and outcome measures in accordance with the Cochrane Handbook.</p><p>Results:The literature search yielded 1295 studies, and 3 studies met the inclusion criteria (n=32;mean age:45.3 years). KFAC stimulation combined with land-based exercise was likely more effective in increasing the quadriceps femoris muscle strength than exercise alone in patients after total knee arthroplasty (TKA). However, KFAC was not superior to low-frequency pulsed current in increasing pedaling work in patients with spinal cord injury. No severe adverse event relevant to KFAC was reported in all 3 studies. The quality of evidence was very low according to the GRADE approach.</p><p>Conclusion:Additional KFAC may be more effective than exercise alone in improving the quadriceps femoris muscle strength in patients after TKA without any severe adverse event. Further studies to investigate the effects of KFAC are warranted to increase the confidence for the effects of KFAC treatment.</p>

    DOI: 10.2490/jjrmc.17023

    CiNii Research

  33. Three-dimensional motion analysis for comprehensive understanding of gait characteristics after sciatic nerve lesion in rodents. International journal

    Junichi Tajino, Akira Ito, Momoko Tanima, Shoki Yamaguchi, Hirotaka Iijima, Akihiro Nakahata, Wataru Kiyan, Tomoki Aoyama, Hiroshi Kuroki

    Scientific reports   Vol. 8 ( 1 ) page: 13585 - 13585   2018.9

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    Rodent models of sciatic nerve lesion are regularly used to assess functional deficits in nerves. Impaired locomotor functions induced by sciatic nerve lesion are currently evaluated with scoring systems despite their limitations. To overcome these shortcomings, which includes low sensitivity, little significance, and the representation of only marginal components of motion profiles, some additional metrics have been introduced. However, a quantitative determination of motion deficits is yet to be established. We used a three-dimensional motion analysis to investigate gait deficits after sciatic nerve lesion in rats. This enabled us to depict the distorted gait motion using both traditional parameters and novel readouts that are specific for the three-dimensional analysis. Our results suggest that three-dimensional motion analysis facilitates a comprehensive understanding of the gait impairment specifically, but not limited to, a sciatic lesion rat model. A broad application of these methods will improve understanding and standardized motor assessment.

    DOI: 10.1038/s41598-018-31579-z

    Web of Science

    Scopus

    PubMed

  34. Psychological health is associated with knee pain and physical function in patients with knee osteoarthritis: an exploratory cross-sectional study. International journal

    Hirotaka Iijima, Tomoki Aoyama, Naoto Fukutani, Takuya Isho, Yuko Yamamoto, Masakazu Hiraoka, Kazuyuki Miyanobu, Masashi Jinnouchi, Eishi Kaneda, Hiroshi Kuroki, Shuichi Matsuda

    BMC psychology   Vol. 6 ( 1 ) page: 19 - 19   2018.5

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    BACKGROUND: Depressive symptoms are a major comorbidity in older adults with knee osteoarthritis (OA). However, the type of activity-induced knee pain associated with depression has not been examined. Furthermore, there is conflicting evidence regarding the association between depression and performance-based physical function. This study aimed to examine (i) the association between depressive symptoms and knee pain intensity, particularly task-specific knee pain during daily living, and (ii) the association between depressive symptoms and performance-based physical function, while considering other potential risk factors, including bilateral knee pain and ambulatory physical activity. METHODS: Patients in orthopaedic clinics (n = 95; age, 61-91 years; 67.4% female) who were diagnosed with radiographic knee OA (Kellgren/Lawrence [K/L] grade ≥ 1) underwent evaluation of psychological health using the Geriatric Depression Scale (GDS). Knee pain and physical function were assessed using the Japanese Knee Osteoarthritis Measure (JKOM), 10-m walk, timed up and go (TUG), and five-repetition chair stand tests. RESULTS: Ordinal logistic regression analysis showed that depression, defined as a GDS score ≥ 5 points, was significantly associated with a worse score on the JKOM pain-subcategory and a higher level of task-specific knee pain intensity during daily living, after being adjusted for age, sex, body mass index (BMI), K/L grade, and ambulatory physical activity. Furthermore, depression was significantly associated with a slower gait velocity and a longer TUG time, after adjusting for age, sex, BMI, K/L grade, presence of bilateral knee pain, and ambulatory physical activity. CONCLUSIONS: These findings indicate that depression may be associated with increased knee pain intensity during daily living in a non-task-specific manner and is associated with functional limitation in patients with knee OA, even after controlling for covariates, including bilateral knee pain and ambulatory physical activity.

    DOI: 10.1186/s40359-018-0234-3

    Scopus

    PubMed

  35. Biomechanical characteristics of stair ambulation in patients with knee OA: A systematic review with meta-analysis toward a better definition of clinical hallmarks. International journal

    Hirotaka Iijima, Kanako Shimoura, Tomoki Aoyama, Masaki Takahashi

    Gait & posture   Vol. 62   page: 191 - 201   2018.5

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    BACKGROUND: Stair climbing was suggested to be the first affected task in individuals with knee osteoarthritis (OA). RESEARCH QUESTION: This review aimed to identify consistent kinematic, kinetic, and spatiotemporal alterations of stair climbing exhibited by individuals with knee OA. METHODS: A literature search published until September 2017 was conducted in PubMed, PEDro, CINAHL, and Cochrane CENTRAL. Reviewer extracted data in accordance with the Cochrane Handbook. Where possible, data were combined into a meta-analysis; the pooled standardized mean differences between individuals with knee OA and healthy adults were calculated using the random-effect model. RESULTS: In total, 585 potentially relevant articles were selected, of which 12 (695 participants, mean age: 58.4 years) met the inclusion criteria. Meta-analysis revealed that kinematic and kinetic alterations during stair climbing associated with knee OA were lower external knee flexion moment in conjunction with a larger trunk/hip flexion angles and smaller knee flexion/ankle dorsiflexion angles. Individuals with knee OA showed a delayed quadriceps activation during stair ascent. A lack of evidence was detected for alterations in external knee adduction moment during stair climbing. Effect estimate in each meta-analysis was judged "very low" on the GRADE approach. SIGNIFICANCE: No strong conclusion can be drawn because of the low quality of evidence; however, individuals with knee OA may exhibit altered kinematics and kinetics changes in sagittal plane during stair climbing, and have delayed quadriceps muscle activity. Further studies with adequate adjustment for confounders are warranted to facilitate clinical hallmarks of the knee OA, particularly in early stages of the disease.

    DOI: 10.1016/j.gaitpost.2018.03.002

    PubMed

  36. Varus thrust visualized during gait was associated with inverted foot in patients with knee osteoarthritis: An exploratory study. International journal

    Hiroshi Ohi, Hirotaka Iijima, Naoto Fukutani, Tomoki Aoyama, Eishi Kaneda, Kazuko Ohi, Hiromu Ito, Shuichi Matsuda, Abe Kaoru

    Gait & posture   Vol. 61   page: 269 - 275   2018.3

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    The foot is speculated to play a role in knee joint kinematics. This exploratory cross-sectional study examined the association between static foot posture and the presence of varus thrust visualized during gait in patients with medial knee osteoarthritis (OA). Patients (n = 88 patients and 134 knees; age, 61-91 years; 68.2% female) with Kellgren/Lawrence (K/L) grade ≥1 in the medial compartment were included in this study and underwent gait observation for varus thrust. These patients' three-dimensional static foot posture while standing was evaluated and their tibiofemoral joint K/L grades and anatomical axis angles were also assessed as covariates. Knees with varus thrust (22 knees, 16.4%) on average had a 4° more inverted calcaneus relative to the floor than those without varus thrust (P < 0.001). A logistic regression analysis showed that an increased calcaneus inversion angle was significantly associated with higher odds of the presence of varus thrust with adjustments for age, sex, body mass index, K/L grade, and anatomical axis angle. The other predictors, such as navicular height, navicular height/foot length, and rearfoot angle relative to the lower leg, were not significantly associated with varus thrust. These results suggest that patients with varus thrust had a different static rearfoot posture as compared with those without varus thrust, a finding that may indicate an important role of static rearfoot posture in the pathogenesis of varus thrust. Furthermore, investigating the potential influence of foot posture on the efficacy of biomechanical interventions, such as lateral wedge insole use, on varus thrust would be of particular interest in the further studies.

    DOI: 10.1016/j.gaitpost.2018.01.020

    Web of Science

    Scopus

    PubMed

  37. Inverted Rearfoot posture in subjects with coexisting patellofemoral osteoarthritis in medial knee osteoarthritis: an exploratory study. International journal

    Hirotaka Iijima, Hiroshi Ohi, Naoto Fukutani, Tomoki Aoyama, Eishi Kaneda, Kaoru Abe, Masaki Takahashi, Shuichi Matsuda

    Journal of foot and ankle research   Vol. 11 ( 1 ) page: 17 - 17   2018

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    BACKGROUND: While abnormal rearfoot posture and its relationship to patellofemoral (PF) pain has been thoroughly discussed in the literature, its relationship to patellofemoral osteoarthritis (PFOA) has not been determined. This study aimed to examine whether rearfoot posture is associated with a higher prevalence of radiographic PFOA in a compartment-specific manner in patients with medial tibiofemoral osteoarthritis (TFOA). METHODS: Participants from orthopedic clinics (n = 68, age 56-90 years, 75.0% female), diagnosed with radiographic medial TFOA (Kellgren/Lawrence [K/L] grade ≥ 2) were included in this study. The presence of PFOA and static rearfoot posture were evaluated using a radiographic skyline view and a footprint automatic measurement apparatus, respectively. The relationship between rearfoot posture and PFOA was examined using analysis of covariance and propensity score-adjusted logistic regression analysis. RESULTS: On average, patients with coexisting PFOA and medial TFOA (n = 39) had an inverted calcaneus 3.1° greater than those with isolated medial TFOA (n = 29). Increased calcaneus inverted angle was significantly associated with a higher probability of the presence of medial PFOA (odds ratio: 1.180, 95% confidence interval: [1.005, 1.439]; p = 0.043). Calcaneus inverted angle was not associated with higher odds of lateral PFOA presence based on the adjusted values. CONCLUSIONS: The presence of an inverted rearfoot was associated with PFOA. Although these findings do not clearly indicate a biomechanical link between rearfoot posture and PFOA, this study shed light on the potential relationship between altered rearfoot posture and PFOA, as can be seen between rearfoot abnormality and PF pain.

    DOI: 10.1186/s13047-018-0261-6

    Web of Science

    Scopus

    PubMed

  38. Postpartum radiographic changes in pelvic morphology and its relation with symptoms of pregnancy-related symphysis pain

    X. Ji, M. Takahashi, S. Morino, T. Takakuwa, H. Iijima, X. Zhang, M. Ishihara, M. Kawagoe, Y. Hatanaka, F. Umezaki, M. Yamashita, T. Tsuboyama, T. Aoyama

    CLINICAL AND EXPERIMENTAL OBSTETRICS & GYNECOLOGY   Vol. 45 ( 5 ) page: 665 - 670   2018

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:I R O G CANADA, INC  

    Introduction: The etiology of pregnancy-related pubic symphysis pain (PSP) is usually considered as the change in pelvic biomechanics during pregnancy. However, the biomechanical changes that occur during puerperium, and the difference of radiographic dimensions in women with different types of PSP remains unknown. Materials and Methods: Fifty women with self-reported PSP were included. Two conventional X-ray radiographic dimensions obtained on the delivery day and one-month postpartum were compared by using paired t-test. Based on the self-reported VAS at one-month postpartum, variables between pain-recovery and non-recovery groups were also compared. Results: The comparison between pre- and post-values indicates a reduced distance between FLAMs (239.1 vs. 237.0 mm), PS separation (7.9 vs. 6.5 mm), and PS translation (4.1 vs. 3.1 mm). No significant differences were observed in the distance between FLAMs, width of PS separation, or pubic symphysial surface (PSS) angle between the recovery and non-recovery groups. However, the non-recovery-group exhibited a significantly large change in PS translation at one-month postpartum than the recovery group (-1.8 vs.-1.1 mm). Conclusions: The pelvic radiography demonstrated a 'closure' alteration in the pelvic cavity diameter one month postpartum with a decrease in the distance between FLAMs and shortened PS separation. The difference in radiographic diameters between groups was not clearly evident.

    DOI: 10.12891/ceog3964.2018

    Web of Science

    Scopus

  39. Comparison of the effects of kilohertz- and low-frequency electric stimulations: A systematic review with meta-analysis. International journal

    Hirotaka Iijima, Masaki Takahashi, Yuto Tashiro, Tomoki Aoyama

    PloS one   Vol. 13 ( 4 ) page: e0195236   2018

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    OBJECTIVE: This study aimed to determine whether kilohertz-frequency alternating current (KFAC) is superior to low-frequency pulsed current (PC) in increasing muscle-evoked torque and lessening discomfort. DATA SOURCES: The electronic databases PubMed, PEDro, CINAHL, and CENTRAL were searched for related articles, published before August 2017. Furthermore, citation search was performed on the original record using Web of Science. REVIEW METHODS: Randomized controlled trials, quasi-experimental studies, and within-subject repeated studies evaluating and comparing KFAC and PC treatments were included. The pooled standardized mean differences (SMDs) of KFAC and PC treatments, with 95% confidence intervals (CIs), were calculated using the random effects model. RESULTS: In total, 1148 potentially relevant articles were selected, of which 14 articles with within-subject repeated designs (271 participants, mean age: 26.4 years) met the inclusion criteria. KFAC did not significantly increase muscle-evoked torque, compared to PC (pooled SMD: -0.25; 95% CI: -0.53, 0.06; P = 0.120). KFAC had comparable discomfort compared to that experienced using PC (pooled SMD: -0.06; 95% CI: -0.50, 0.38; P = 0.800). These estimates of the effects had a high risk of bias, as assessed using the Downs and Black scale, and were highly heterogeneous studies. CONCLUSIONS: This meta-analysis does not establish that KFAC is superior to PC in increasing muscle-evoked torque and lessening discomfort level. However, no strong conclusion could be drawn because of a high risk of bias and a large amount of heterogeneity. High quality studies comparing the efficacy between PC and KFAC treatments with consideration of potential confounders is warranted to facilitate the development of effective treatment.

    DOI: 10.1371/journal.pone.0195236

    PubMed

  40. Effectiveness of mesenchymal stem cells for treating patients with knee osteoarthritis: a meta-analysis toward the establishment of effective regenerative rehabilitation. International journal

    Hirotaka Iijima, Takuya Isho, Hiroshi Kuroki, Masaki Takahashi, Tomoki Aoyama

    NPJ Regenerative medicine   Vol. 3   page: 15 - 15   2018

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    This systematic review with a meta-analysis aimed to summarize the current evidence of the effectiveness of mesenchymal stem cell (MSC) treatment for knee osteoarthritis (OA) and to examine whether rehabilitation is an effect modifier of the effect estimate of MSC treatment. A literature search yielded 659 studies, of which 35 studies met the inclusion criteria (n = 2385 patients; mean age: 36.0-74.5 years). The meta-analysis results suggested that MSC treatment through intra-articular injection or arthroscopic implantation significantly improved knee pain (standardized mean difference [SMD]: -1.45, 95% confidence interval [CI]: -1.94, -0.96), self-reported physical function (SMD: 1.50, 95% CI: 1.09, 1.92), and cartilage quality (SMD: -1.99; 95% CI: -3.51, -0.47). However, the MSC treatment efficacy on cartilage volume was limited (SMD: 0.49; 95% CI: -0.19, 1.16). Minor adverse events (knee pain or swelling) were reported with a wide-ranging prevalence of 2-60%; however, no severe adverse events occurred. The evidence for these outcomes was "very low" to "low" according to the Grades of Recommendation, Assessment, Development and Evaluation system because of the poor study design, high risk of bias, large heterogeneity, and wide 95% CI of the effects estimate. Performing rehabilitation was significantly associated with better SMD for self-reported physical function (regression coefficient: 0.881, 95% CI: 0.049, 1.712; P = 0.039). We suggest that more high quality randomized controlled trials with consideration of the potential rehabilitation-driven clinical benefit would be needed to facilitate the foundation of effective MSC treatment and regenerative rehabilitation for patients with knee OA.

    DOI: 10.1038/s41536-018-0041-8

    PubMed

  41. Association of bilateral flat feet with knee pain and disability in patients with knee osteoarthritis: A cross-sectional study. International journal

    Hirotaka Iijima, Hiroshi Ohi, Takuya Isho, Tomoki Aoyama, Naoto Fukutani, Eishi Kaneda, Kazuko Ohi, Kaoru Abe, Hiroshi Kuroki, Shuichi Matsuda

    Journal of orthopaedic research : official publication of the Orthopaedic Research Society   Vol. 35 ( 11 ) page: 2490 - 2498   2017.11

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    This cross-sectional study examined the relationship of flat feet with knee pain, disability, and physical performance in patients with knee osteoarthritis (OA). Orthopedic clinic participants (n = 95; age 61-91 years; 68.4% women) with Kellgren-Lawrence (K/L) grade ≥1 in the medial compartment underwent evaluation of navicular height and foot length for flat feet. Knee pain intensity, disability, and physical performance were evaluated using the Japanese Knee Osteoarthritis Measure, 10-m walk, timed up and go, and five-repetition chair stand tests. Of the 95 enrolled patients, 24 (25.3%) had bilateral flat feet, and significantly higher knee pain compared to patients with no flat feet (11.3 ± 8.23 points vs. 6.58 ± 6.37 points; p = 0.043). A ordinal logistic regression analysis showed that bilateral flat feet were significantly associated with increased knee pain (proportional odds ratio: 5.48, 95% confidence interval: 1.96, 15.3; p = 0.001) compared with no flat feet, adjusted for age, sex, body mass index, and tibiofemoral joint K/L grade, which is consistent across various different cutoffs of the definition of flat feet. Physical performance was similar between patients with and without bilateral flat feet. The presence of unilateral flat feet was not significantly associated with any outcome measures. These findings indicate that bilateral, but not unilateral, flat feet are associated with worse knee pain. A prospective study investigating a causal relationship between bilateral flat feet posture and knee pain as well as disability would be of particular interest to verify the potential adverse effect of altered foot posture. © 2017 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 35:2490-2498, 2017.

    DOI: 10.1002/jor.23565

    PubMed

  42. Low back pain and causative movements in pregnancy: a prospective cohort study. International journal

    Saori Morino, Mika Ishihara, Fumiko Umezaki, Hiroko Hatanaka, Hirotaka Iijima, Mamoru Yamashita, Tomoki Aoyama, Masaki Takahashi

    BMC musculoskeletal disorders   Vol. 18 ( 1 ) page: 416 - 416   2017.10

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    BACKGROUND: Low back pain (LBP) during pregnancy might be strongly related to posture and movements of the body, and its management is a clinically important issue. The purpose of this study was to investigate the activities related to LBP during pregnancy. METHODS: Participants included 275 women before 12 weeks of pregnancy. The women were evaluated at 12, 24, 30, and 36 weeks of pregnancy. The intensity of LBP was assessed using the Numerical Rating Scale (NRS). Movements related to LBP were investigated by free descriptive answers. Descriptive statistics were used to compile the movements that pregnant women thought induced LBP at each evaluation. Subsequently, a linear regression analysis was performed to evaluate the degree of association of certain movements with LBP using the data of participants who had LBP. The intensity of LBP (NRS score) was specified as the dependent variable, the movements that were related to pain were specified as the independent variables at the analysis. A significance threshold was set at 0.05. RESULTS: The final sample used in the analyses was 254, 249, 258, and 245 women at 12, 24, 30, and 36 weeks of pregnancy, respectively. There were 16 kinds of movements that induced LBP and all of them were daily activities rather than special movements that require extra task or effort. As pregnancy progressed, less number of participants attributed pain to a specific movement. At all evaluations, movements, especially sitting up, standing up from a chair, and tossing and turning were thought to be related to LBP. Furthermore, standing up from a chair and tossing and turning were significantly related to LBP throughout the pregnancy. In contrast, lying down and sitting up were significantly related to LBP but the relationship did not continue till late pregnancy. CONCLUSIONS: Daily routine activity is related to LBP during pregnancy. These results suggest that recommendations for pregnant women about basic physical movements, such as ways of standing up that reduce the load on the body might be useful in the management of LBP.

    DOI: 10.1186/s12891-017-1776-x

    Web of Science

    Scopus

    PubMed

    CiNii Research

  43. Association of varus thrust with prevalent patellofemoral osteoarthritis: A cross-sectional study. International journal

    Hirotaka Iijima, Naoto Fukutani, Yuko Yamamoto, Masakazu Hiraoka, Kazuyuki Miyanobu, Masashi Jinnouchi, Eishi Kaneda, Takuya Isho, Tomoki Aoyama, Hiroshi Kuroki, Shuichi Matsuda

    Gait & posture   Vol. 58   page: 394 - 400   2017.10

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    This cross-sectional study investigated (i) the association of varus thrust during gait with the presence of patellofemoral osteoarthritis (PFOA) in patients with medial knee osteoarthritis (OA) and (ii) patellar alignment in the knees with varus thrust. Participants from orthopedic clinics (n=171; mean age, 73.4 years; 71.9% female) diagnosed with radiographic medial knee OA (Kellgren/Lawrence [K/L] grade ≥1) were included in this study, and underwent gait observation for varus thrust assessment using 2D video analysis. A radiographic skyline view was used to assess the presence of medial PFOA using the grading system from the Osteoarthritis Research Society International Atlas. The tibiofemoral joint K/L grade, patellar alignment (i.e., lateral shift and tilting angle), and knee pain intensity were also evaluated as covariates. Thirty-two (18.7%) of 171 patients exhibited varus thrust and they presented significantly higher knee pain (46.0±3.04mm vs. 32.4±2.73mm; P=0.024), a lower patellar tilting angle (P=0.024), and a higher prevalence of PFOA compared with those without varus thrust. A logistic regression analysis with adjustment of covariates showed that varus thrust was significantly associated with higher odds of the presence of mixed and medial PFOA, and trended to significantly associate with any PFOA, including lateral PFOA. This indicates that varus thrust was associated with PFOA in a compartment-nonspecific manner in patients with medial knee OA. Varus thrust may represent a clinical disease feature of more advanced and multicompartmental disease.

    DOI: 10.1016/j.gaitpost.2017.08.033

    PubMed

  44. Coexisting lateral tibiofemoral osteoarthritis is associated with worse knee pain in patients with mild medial osteoarthritis. International journal

    H Iijima, T Aoyama, K Nishitani, H Ito, N Fukutani, T Isho, E Kaneda, H Kuroki, S Matsuda

    Osteoarthritis and cartilage   Vol. 25 ( 8 ) page: 1274 - 1281   2017.8

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    OBJECTIVE: To examine the clinical impact of coexisting lateral osteoarthritis (OA) in knees with mild medial OA. DESIGN: In patients with Kellgren/Lawrence (K/L) grade 2 OA in the medial compartment (n = 100; age: 56-89 years; 80.0% female), anteroposterior knee radiography was used to assess the presence of lateral OA, using grading systems from the Osteoarthritis Research Society International (OARSI) atlas and the K/L classification. The Japanese Knee Osteoarthritis Measure (JKOM), knee range of motion (ROM), and performance-based functional measures (10 m walk, timed up and go and five repetition chair stand maneuvers) were evaluated. The outcomes were compared between patients with and without lateral OA using an analysis of covariance (ANCOVA) or nonparametric rank ANCOVA. Furthermore, ordinal logistic regression analysis was performed, with responses on individual JKOM pain questionnaires as the outcomes and lateral OA as the predictor. RESULTS: Knees with coexisting lateral OA had a significantly worse score of JKOM pain question compared with those without, after adjusting for covariates. The presence of lateral OA was significantly associated with knee pain while ascending/descending stairs and standing. These results were consistent between different definitions of the K/L and OARSI grading systems. The knee ROM and performance-based functional measures were not significantly different between patients with and without lateral OA. CONCLUSION: Knees with concomitant lateral and mild medial OA may be more symptomatic compared to those without lateral OA. These findings might help to define a clinically distinct subgroup based on a simple radiographic finding in mild knee OA.

    DOI: 10.1016/j.joca.2017.02.801

    PubMed

  45. Relationship Between Pedometer-Based Physical Activity and Physical Function in Patients With Osteoarthritis of the Knee: A Cross-Sectional Study. International journal

    Hirotaka Iijima, Naoto Fukutani, Takuya Isho, Yuko Yamamoto, Masakazu Hiraoka, Kazuyuki Miyanobu, Masashi Jinnouchi, Eishi Kaneda, Tomoki Aoyama, Hiroshi Kuroki, Shuichi Matsuda

    Archives of physical medicine and rehabilitation   Vol. 98 ( 7 ) page: 1382 - 1388   2017.7

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    OBJECTIVE: To examine the association between pedometer-based ambulatory physical activity (PA) and physical function in patients with knee osteoarthritis (OA). DESIGN: Cross-sectional observational study. SETTING: Institutional practice. PARTICIPANTS: Participants in orthopedic clinics (N=207; age, 56-90y; 71.5% women) with diagnosed radiographic knee OA (Kellgren/Lawrence [K/L] grade ≥1). INTERVENTIONS: Not applicable. MAIN OUTCOME MEASURES: Ambulatory PA was objectively measured as steps per day. Physical function was assessed using the Japanese Knee Osteoarthritis Measure (JKOM) functional subcategory, 10-m walk, Timed Up and Go (TUG), and 5-repetition chair stand (5CS) tests. RESULTS: Patients walking <2500 steps/d had a low level of physical function with a slower gait speed, longer TUG time, and worse JKOM functional score compared with those who walk 2500 to 4999, 5000 to 7499, and ≥7500 steps/d adjusted for age, sex, body mass index [BMI], and K/L grade. Ordinal logistic regression analysis revealed that steps per day (continuous) was associated with better physical function adjusted for age, sex, BMI, and K/L grade. These relationships were still robust in sensitivity analyses that included patients with K/L grades ≥2 (n=140). CONCLUSIONS: Although increased ambulatory PA had a positive relationship with better physical function, walking <2500 steps/d may be a simple indicator for a decrease in physical function in patients with knee OA among standard PA categories. Our findings might be a basis for counseling patients with knee OA about their ambulatory PA and for developing better strategies for improving physical function in sedentary patients with knee OA.

    DOI: 10.1016/j.apmr.2016.12.021

    Web of Science

    Scopus

    PubMed

  46. Association of frontal plane knee alignment with foot posture in patients with medial knee osteoarthritis. International journal

    Hiroshi Ohi, Hirotaka Iijima, Tomoki Aoyama, Eishi Kaneda, Kazuko Ohi, Kaoru Abe

    BMC musculoskeletal disorders   Vol. 18 ( 1 ) page: 246 - 246   2017.6

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    BACKGROUND: To examine the association of radiographic frontal plane knee alignment with three-dimensional foot posture in patients with medial knee osteoarthritis (OA). METHODS: Participants in orthopedic clinics with Kellgren/Lawrence (K/L) grade ≥1 (88 patients and 88 knees; age, 61-91 years; 65.9% female) were enrolled. An anteroposterior radiographic view was used to assess the anatomical axis angle (AAA) after subtracting a sex-specific correction factor. The three-dimensional foot posture was also evaluated. RESULTS: Multiple regression analyses showed that increased corrected AAA (i.e., valgus direction) was independently associated with a decrease in the hallux valgus angle (regression coefficient: -0.40 per degree, 95% confidence interval [CI]: -0.72, -0.09; P = 0.013) and increase in the pronation angle of the calcaneus relative to floor (regression coefficient: 0.33 per degree, 95% CI: 0.10, 0.56; P = 0.005) adjusted for age, sex, and body mass index. The relationship between the corrected AAA and hallux valgus angle strengthened (regression coefficient: -0.60 per degree, 95% CI: -1.08, -0.13; P = 0.014) in varus-aligned knees examined separately (63 knees). The other foot postures (navicular height, navicular height/foot length, and rearfoot angle) were not significantly associated with corrected AAA. CONCLUSIONS: Radiographic frontal plane knee alignment was associated with hallux valgus angle and calcaneus angle relative to the floor in patients with medial knee OA, particularly in varus-aligned knees. These results indicate a connection between altered frontal knee alignment and foot posture, which may be helpful in understanding the pathogenesis of altered foot posture observed in patients with knee OA.

    DOI: 10.1186/s12891-017-1588-z

    PubMed

  47. Pathohistological investigation of osteochondral tissue obtained during total knee arthroplasty after osteochondral autologous transfer: a case report. International journal

    Momoko Tanima-Nagai, Hideto Harada, Tomoki Aoyama, Shoki Yamaguchi, Akira Ito, Junichi Tajino, Hirotaka Iijima, Xiankai Zhang, Hiroshi Kuroki, Masahiko Kobayashi

    BMC research notes   Vol. 10 ( 1 ) page: 194 - 194   2017.6

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    BACKGROUND: Osteochondral autologous transfer is one of the repair techniques for cartilage defects of knee with promising knee function recovery. There are no reports including histopathological images concerning human osteochondral tissue after osteochondral autologous transfer. This is the first report to present pathohistological findings of transplanted plugs and host tissues extracted from the human body 3 years after osteochondral autologous transfer. This study aimed to explore the cause factor of chronic pain using histological techniques. CASE PRESENTATION: A 67-year-old Japanese man presented with adjusted total knee arthroplasty 3 years after osteochondral autologous transfer. Although in pain, arthroscopic assessment was not severe. The specimens which was gained during total knee arthroplasty were investigated in gross and microscopically using immunohistochemical staining technic. Histological examination revealed that the gap between grafted plugs and host osteochondral tissues was filled with fibrous tissue that stained positive for type I collagen. A degenerative change and some neovascularity were observed in the regenerated tissue and host trabecular bone. Furthermore, cysts and bone marrow edema were observed. CONCLUSION: Our data suggests that the host osteochondral morbidity around grafted plugs might be related to chronical pain and revision surgery.

    DOI: 10.1186/s13104-017-2513-0

    Scopus

    PubMed

  48. Physiological exercise loading suppresses post-traumatic osteoarthritis progression via an increase in bone morphogenetic proteins expression in an experimental rat knee model. International journal

    H Iijima, A Ito, M Nagai, J Tajino, S Yamaguchi, W Kiyan, A Nakahata, J Zhang, T Wang, T Aoyama, K Nishitani, H Kuroki

    Osteoarthritis and cartilage   Vol. 25 ( 6 ) page: 964 - 975   2017.6

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    OBJECTIVE: To evaluate the dose-response relationship of exercise loading in the cartilage-subchondral bone (SB) unit in surgically-induced post-traumatic osteoarthritis (PTOA) of the knee. DESIGN: Destabilized medial meniscus (DMM) surgery was performed on the right knee of 12-week-old male Wistar rats, and sham surgery was performed on the contralateral knee. Four weeks after the surgery, the animals were subjected to moderate (12 m/min) or intense (21 m/min) treadmill exercises for 30 min/day, 5 days/week for 4 weeks. PTOA development in articular cartilage and SB was examined using histological and immunohistochemical analyses, micro-computed tomography (micro-CT) analysis, and biomechanical testing at 8 weeks after surgery. Gremlin-1 was injected to determine the role of bone morphogenetic protein (BMP) signaling on PTOA development following moderate exercise. RESULTS: Moderate exercise increased BMP-2, BMP-4, BMP-6, BMP receptor 2, pSmad-5, and inhibitor of DNA binding protein-1 expression in the superficial zone chondrocytes and suppressed cartilage degeneration, osteophyte growth, SB damage, and osteoclast-mediated SB resorption. However, intense exercise had little effect on BMP expression and even caused progression of these osteoarthritis (OA) changes. Gremlin-1 injection following moderate exercise caused progression of the PTOA development down to the level of the non-exercise DMM-operated knee. CONCLUSIONS: Exercise regulated cartilage-SB PTOA development in DMM-operated knees in a dose-dependent manner. Our findings shed light on the important role of BMP expression in superficial zone chondrocytes in attenuation of PTOA development following physiological exercise loading. Further studies to support a mechanism by which BMPs would be beneficial in preventing PTOA progression are warranted.

    DOI: 10.1016/j.joca.2016.12.008

    Web of Science

    Scopus

    PubMed

  49. Changes in clinical symptoms and functional disability in patients with coexisting patellofemoral and tibiofemoral osteoarthritis: a 1-year prospective cohort study. International journal

    Hirotaka Iijima, Naoto Fukutani, Takuya Isho, Yuko Yamamoto, Masakazu Hiraoka, Kazuyuki Miyanobu, Masashi Jinnouchi, Eishi Kaneda, Tomoki Aoyama, Hiroshi Kuroki, Shuichi Matsuda

    BMC musculoskeletal disorders   Vol. 18 ( 1 ) page: 126 - 126   2017.3

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    BACKGROUND: This 1-year prospective cohort study aimed to compare the changes in clinical symptoms and functional disability between patients with coexisting patellofemoral (PF) and tibiofemoral (TF) osteoarthritis (OA) and those with isolated TFOA. METHODS: Seventy-two patients with medial knee OA were enrolled. Knee pain and functional disability were assessed at baseline and at 1-year follow-up using the Japanese Knee Osteoarthritis Measure (JKOM) and a visual analog scale (VAS). We performed two-way analysis of covariance for the clinical outcome variables to examine, time (baseline and follow-up), group (coexisting PFOA and isolated TFOA), and time-group interaction effects. Furthermore, we conducted post-hoc exploratory analysis to address the possibility that dividing patients according to location of PFOA (i.e., isolated lateral, isolated medial, and mixed [bilateral]) may identify a distinct subgroup with different changes in clinical outcomes at 1-year follow-up. RESULTS: We detected group effects only in scores of the JKOM pain subscale (P = 0.012) and VAS (P = 0.033), adjusted for age, sex, and body mass index. Patients with coexisting PFOA have stable moderate level knee pain and functional disability throughout the year which is significantly worse than that in those with isolated TFOA. Post-hoc subgroup analysis demonstrated that change of knee pain likely varied with location of PFOA. Patients with isolated lateral PFOA had mild/moderate level knee pain, and their VAS scores were likely to improve, whereas those with mixed PFOA exhibited stable to worsening moderate/severe knee pain. CONCLUSIONS: Although we did not detect differences in changes in clinical symptoms and functional disability between patients with coexisting PFOA and those with isolated TFOA, our findings indicate that patients with coexisting PFOA had worse clinical symptoms and functional disability than those with isolated TFOA. The results of the exploratory analysis suggested that patients with coexisting PFOA might have heterogeneous clinical outcomes, and presence of mixed PFOA might be an indicator of severe clinical knee OA.

    DOI: 10.1186/s12891-017-1486-4

    PubMed

  50. Effect of Low-Intensity Pulsed Ultrasound after Mesenchymal Stromal Cell Injection to Treat Osteochondral Defects: An In Vivo Study. International journal

    Shoki Yamaguchi, Tomoki Aoyama, Akira Ito, Momoko Nagai, Hirotaka Iijima, Junichi Tajino, Xiangkai Zhang, Kiyan Wataru, Hiroshi Kuroki

    Ultrasound in medicine & biology   Vol. 42 ( 12 ) page: 2903 - 2913   2016.12

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    We investigated the effect of low-intensity pulsed ultrasound (LIPUS) treatment combined with mesenchymal stromal cell (MSC) injection for cartilage repair and subchondral bone reconstitution for treatment of osteochondral defects. An osteochondral defect was created on both femur grooves of Wistar rats. Four weeks later, bone marrow MSCs were injected into the right knee joint. The rats were divided into two intervention groups: without or with LIPUS irradiation. Cartilage repair was evaluated histologically based on the Wakitani cartilage repair score. Subchondral bone reconstitution was evaluated as bone volume (BV)/tissue volume (TV) by micro-computed tomography analysis. MSC injection improved the cartilage repair score, and LIPUS irradiation improved BV/TV. Combination treatment promoted both cartilage repair and BV/TV improvement. Thus, MSC injection combined with LIPUS irradiation is more effective than either treatment alone in promoting concurrent cartilage repair and subchondral reconstitution.

    DOI: 10.1016/j.ultrasmedbio.2016.07.021

    PubMed

  51. Knee pain during activities of daily living and its relationship with physical activity in patients with early and severe knee osteoarthritis. International journal

    Naoto Fukutani, Hirotaka Iijima, Tomoki Aoyama, Yuko Yamamoto, Masakazu Hiraoka, Kazuyuki Miyanobu, Masashi Jinnouchi, Eishi Kaneda, Tadao Tsuboyama, Shuichi Matsuda

    Clinical rheumatology   Vol. 35 ( 9 ) page: 2307 - 16   2016.9

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    This study aimed to investigate whether knee pain during various activities of daily living (ADLs) is associated with physical activity in patients with early and severe knee osteoarthritis (OA). We hypothesized that the painful ADLs associated with decreased physical activity differ according to disease severity. This cross-sectional study enrolled 270 patients with medial knee OA, assigned to either the early (Kellgren Lawrence [K/L] grade 1-2) or the severe group (K/L grade 3-4). Physical activity was assessed using a pedometer. Knee pain during six ADLs (waking up in the morning, walking on a flat surface, ascending stairs, etc.) was evaluated using a questionnaire. We performed multiple regression and quantile regression analysis to investigate whether knee pain during each ADL was associated with physical activity. In the early group, the more knee pain they experienced while ascending stairs, the lower their physical activity was (75th regression coefficient = -1033.70, P = 0.018). In the severe group, the more knee pain they experienced while walking on a flat surface or bending to the floor or standing up, the lower their physical activity was (unstandardized coefficients = -1850.87, P = 0.026; unstandardized coefficients = -2640.35, P = 0.010). Knee pain while ascending stairs and while walking on a flat surface or bending to the floor or standing up was a probable limiting factor for physical activity in early and severe knee OA, respectively. These findings suggested that a reduction in task-specific knee pain according to disease severity could improve physical activity levels.

    DOI: 10.1007/s10067-016-3251-8

    Web of Science

    Scopus

    PubMed

  52. Remobilization causes site-specific cyst formation in immobilization-induced knee cartilage degeneration in an immobilized rat model. International journal

    Momoko Nagai, Akira Ito, Junichi Tajino, Hirotaka Iijima, Shoki Yamaguchi, Xiangkai Zhang, Tomoki Aoyama, Hiroshi Kuroki

    Journal of anatomy   Vol. 228 ( 6 ) page: 929 - 39   2016.6

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    An understanding of the articular cartilage degenerative process is necessary for the prevention and treatment of joint disease. The present study aimed to examine how long-term immobilization-induced cartilage degeneration is aggravated by remobilization. Sixty 8-week-old male Wistar rats were used in this study. The unilateral knee joint was immobilized using an external fixator for 8 weeks. The rats were killed at 0 and 3 days, and at 1, 2, 4 and 8 weeks after removing the fixator. After the rats were killed, the maximum knee extension angles were measured. Histological sections at the medial mid-condylar region (non-contact, transitional and contact regions of the femur and tibia) were prepared and scored. The cartilage thickness and number of chondrocytes were measured, and CD44 and Col2-3/4c expression levels were assessed immunohistochemically. The histological assessment revealed progressive aggravation of cartilage degeneration in the transitional region, with a decreased number of chondrocytes and CD44-positive chondrocytes as well as poor scoring over time, particularly in the tibia. Cyst formation was confirmed in the transitional region of the tibia at 8 weeks post-remobilization. The cartilage thickness in the transitional region was thicker than that in the contact region, particularly in the tibia. Col2-3/4c expression was observed in the non-contact and transitional regions, and the knee extension angle was recovered. In conclusion, immobilization-induced cartilage degeneration was aggravated by remobilization over time in the transitional region, followed by observations of a decreased number of chondrocytes and morphological disparity between different cartilage regions.

    DOI: 10.1111/joa.12453

    Web of Science

    Scopus

    PubMed

  53. Exercise intervention increases expression of bone morphogenetic proteins and prevents the progression of cartilage-subchondral bone lesions in a post-traumatic rat knee model. International journal

    H Iijima, T Aoyama, A Ito, J Tajino, S Yamaguchi, M Nagai, W Kiyan, X Zhang, H Kuroki

    Osteoarthritis and cartilage   Vol. 24 ( 6 ) page: 1092 - 102   2016.6

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    OBJECTIVE: This study aimed to determine whether treadmill walking (TW) prevents the progression of post-traumatic osteoarthritic changes in cartilage-subchondral bone unit, and whether the exercise timing changes the exercise efficacy in destabilized medial meniscus (DMM) rat knees. DESIGN: Twelve-week-old male Wistar rats underwent DMM surgery on their right knees and sham surgery on their left knees and were assigned to either the sedentary (n = 10) or walking (n = 24) groups. The rats in the walking group were subjected to TW from day 2 through 4 weeks, from 4 through 8 weeks, or from day 2 through 8 weeks (n = 8 per group). Osteoarthritic changes of cartilage and subchondral bone were assessed with micro-computed tomography, histology, and immunohistochemistry 8 weeks after surgery. RESULTS: TW prevented the progression of cartilage and subchondral bone lesions induced by the DMM, and increased bone morphogenetic protein (BMP)-2 and -6 expressions in superficial zone chondrocytes and bone-lining cells including osteoblasts. Furthermore, the TW-induced increase in BMPs varied with the exercise timing. Beginning TW 4 weeks after DMM surgery was the best option for increasing BMPs, coinciding with the most robust prevention of osteoarthritic changes. CONCLUSIONS: TW increased the expression of BMPs and prevented the progression of cartilage-subchondral bone lesions in rat knees with a DMM. Selective exercise timing may be a key factor in the development of an exercise regimen for preventing the progression of post-traumatic osteoarthritis (PTOA). Furthermore, exercise may have favorable effects even after the PTOA has been developed.

    DOI: 10.1016/j.joca.2016.01.006

    PubMed

  54. Clinical Impact of Coexisting Patellofemoral Osteoarthritis in Japanese Patients With Medial Knee Osteoarthritis. International journal

    Hirotaka Iijima, Naoto Fukutani, Tomoki Aoyama, Takahiko Fukumoto, Daisuke Uritani, Eishi Kaneda, Kazuo Ota, Hiroshi Kuroki, Shuichi Matsuda

    Arthritis care & research   Vol. 68 ( 4 ) page: 493 - 501   2016.4

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    OBJECTIVE: To evaluate the clinical impact in knee osteoarthritis (OA) of coexisting patellofemoral (PF) joint OA (PFOA) in Japanese patients with medial tibiofemoral (TF) joint OA (TFOA). METHODS: Patients with medial knee OA (n = 143) were enrolled. The radiographic severity of the TF and PF joints, anatomic axis angle of the TF joint, patellar alignment, trochlear morphology, patellar height, and passive range of motion (ROM) of the painful knee were evaluated. Additionally, the Japanese Knee Osteoarthritis Measure (JKOM) was used to investigate the association between the presence of PFOA and clinical symptoms. RESULTS: PFOA was present in 98 of 143 patients (68.5%) with medial knee OA. Quantile regression analysis revealed that coexisting PFOA was associated with the pain-related subcategory of the JKOM. Furthermore, multiple logistic regression analysis showed that coexisting PFOA was associated with higher odds of reporting knee pain on using stairs while ascending (odds ratio [OR] 4.81 [95% confidence interval (95% CI) 1.73, 14.3]) and descending (OR 3.86 [95% CI 1.44, 10.8]). A more varus knee alignment and lower knee flexion ROM, which are features of patients with coexisting PFOA, were associated with knee pain while ascending/descending stairs. However, patellar alignment, trochlear morphology, and patellar height were not significantly associated with knee pain. CONCLUSION: PFOA coexisting with medial knee OA was associated with worse clinical symptoms, particularly while ascending/descending stairs, although patellar alignment did not contribute to reporting knee pain. Further studies that include evaluation of the PF joint are warranted to develop a basis for an optimal intervention based on compartmental involvement.

    DOI: 10.1002/acr.22691

    PubMed

  55. The Effect of Exercise on the Early Stages of Mesenchymal Stromal Cell-Induced Cartilage Repair in a Rat Osteochondral Defect Model

    Yamaguchi Shoki, Aoyama Tomoki, Ito Akira, Nagai Momoko, Iijima Hirotaka, Tajino Junichi, Zhang Xiangkai, Kiyan Wataru, Kuroki Hiroshi

    PLOS ONE   Vol. 11 ( 3 )   2016.3

     More details

    Language:English  

    The repair of articular cartilage is challenging owing to the restriction in the ability of articular cartilage to repair itself. Therefore, cell supplementation therapy is possible cartilage repair method. However, few studies have verified the efficacy and safety of cell supplementation therapy. The current study assessed the effect of exercise on early the phase of cartilage repair following cell supplementation utilizing mesenchymal stromal cell (MSC) intra-articular injection. An osteochondral defect was created on the femoral grooves bilaterally of Wistar rats. Mesenchymal stromal cells that were obtained from male Wistar rats were cultured in monolayer. After 4 weeks, MSCs were injected into the right knee joint and the rats were randomized into an exercise or no-exercise intervention group. The femurs were divided as follows: C group (no exercise without MSC injection); E group (exercise without MSC injection); M group (no exercise with MSC injection); and ME group (exercise with MSC injection). At 2, 4, and 8 weeks after the injection, the femurs were sectioned and histologically graded using the Wakitani cartilage repair scoring system. At 2 weeks after the injection, the total histological scores of the M and ME groups improved significantly compared with those of the C group. Four weeks after the injection, the scores of both the M and ME groups improved significantly. Additionally, the scores in the ME group showed a significant improvement compared to those in the M group. The improvement in the scores of the E, M, and ME groups at 8 weeks were not significantly different. The findings indicate that exercise may enhance cartilage repair after an MSC intra-articular injection. This study highlights the importance of exercise following cell transplantation therapy.

    CiNii Research

  56. Subchondral plate porosity colocalizes with the point of mechanical load during ambulation in a rat knee model of post-traumatic osteoarthritis. International journal

    H Iijima, T Aoyama, J Tajino, A Ito, M Nagai, S Yamaguchi, X Zhang, W Kiyan, H Kuroki

    Osteoarthritis and cartilage   Vol. 24 ( 2 ) page: 354 - 63   2016.2

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    OBJECTIVE: This study investigated the association between spatiotemporal cartilage-subchondral bone plate alterations and mechanical load during ambulation in an experimental rat model of destabilized medial meniscus (DMM). DESIGN: Twelve-week-old Wistar rats (n = 38) underwent DMM surgery on the right knee and sham surgery on the left knee. At 2 and 4 weeks after surgery, subchondral bone changes were evaluated via micro-computed tomography with various knee flexion angles to simulate weight-bearing during rat ambulation under a 3-dimensional motion capture apparatus. Additionally, the biomechanical properties, histology, and ultrastructure of the medial tibia and femoral condyle were evaluated. RESULTS: Focal subchondral bone plate perforations were confirmed in the medial tibia within 2 weeks after surgery and were aggravated rapidly 2 weeks later. This subchondral plate porosity colocalized with articular cartilage lesions as confirmed by histology and scanning electron microscopy, and coincided with the likely point of contact between the posterior femoral condyle and tibial plateau during ambulation. Biomechanical properties were confirmed at the medial tibia, at which stiffness was reduced to approximately half that of the sham-operated knee at 4 weeks after surgery. CONCLUSIONS: Cartilage-subchondral bone plate alterations localized in the region of the point of mechanical load during ambulation in DMM-operated knees, at which the mechanical integrity of cartilage was impaired. These results indicate that DMM-induced increases in mechanical load play an important role in the pathogenesis of early post-traumatic osteoarthritis (OA), and it might accelerate the development of the disease via cartilage-subchondral bone plate crosstalk through increased subchondral plate perforations.

    DOI: 10.1016/j.joca.2015.09.001

    Web of Science

    Scopus

    PubMed

  57. Association of Varus Thrust With Pain and Stiffness and Activities of Daily Living in Patients With Medial Knee Osteoarthritis. International journal

    Naoto Fukutani, Hirotaka Iijima, Takahiko Fukumoto, Daisuke Uritani, Eishi Kaneda, Kazuo Ota, Tomoki Aoyama, Tadao Tsuboyama, Shuichi Matsuda

    Physical therapy   Vol. 96 ( 2 ) page: 167 - 75   2016.2

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    BACKGROUND: Increasing evidence highlights potential associations between varus thrust and health domains associated with knee osteoarthritis (OA). OBJECTIVE: The aim of this study was to investigate the association between varus thrust and 2 subcategories-"pain and stiffness" and "activities of daily living (ADL)"-of the Japanese Knee Osteoarthritis Measure (JKOM). DESIGN: This was a cross-sectional study. METHODS: In total, 296 outpatients with knee OA visiting orthopedic clinics were enrolled. The inclusion criteria were age ≥50 years, medial knee OA and Kellgren-Lawrence (K/L) grade ≥1 in one or both knees, and the ability to walk independently. Standard posterior-anterior knee radiographs were measured for varus alignment. Participants were video recorded while walking and were evaluated for the presence or absence of varus thrust. Pain and stiffness of the knee joint and ADL were evaluated using the JKOM. Multivariate regressions (outcomes: pain and stiffness and ADL; predictor variable: varus thrust) were performed. RESULTS: Varus thrust was present in 46 (16.2%) of 284 patients. Multivariate regression analyses demonstrated that varus thrust is independently associated with pain and stiffness, adjusted for age, sex, body mass index, K/L grade, and varus alignment (β=.17, P=.005). However, the association between varus thrust and ADL was not significant (β=.11, P=.058). Based on sensitivity analyses, including participants of K/L grade 1 had little influence on this analysis. LIMITATIONS: Only 16.2% of participants had a varus thrust. Moreover, a cause-effect relationship between varus thrust and pain and stiffness remains unknown due to the cross-sectional design of this study. CONCLUSIONS: Varus thrust was associated with pain and stiffness in patients with medial knee OA. However, the association between varus thrust and ADL did not reach significance.

    DOI: 10.2522/ptj.20140441

    PubMed

  58. The Effect of Exercise on the Early Stages of Mesenchymal Stromal Cell-Induced Cartilage Repair in a Rat Osteochondral Defect Model. International journal

    Shoki Yamaguchi, Tomoki Aoyama, Akira Ito, Momoko Nagai, Hirotaka Iijima, Junichi Tajino, Xiangkai Zhang, Wataru Kiyan, Hiroshi Kuroki

    PloS one   Vol. 11 ( 3 ) page: e0151580   2016

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    The repair of articular cartilage is challenging owing to the restriction in the ability of articular cartilage to repair itself. Therefore, cell supplementation therapy is possible cartilage repair method. However, few studies have verified the efficacy and safety of cell supplementation therapy. The current study assessed the effect of exercise on early the phase of cartilage repair following cell supplementation utilizing mesenchymal stromal cell (MSC) intra-articular injection. An osteochondral defect was created on the femoral grooves bilaterally of Wistar rats. Mesenchymal stromal cells that were obtained from male Wistar rats were cultured in monolayer. After 4 weeks, MSCs were injected into the right knee joint and the rats were randomized into an exercise or no-exercise intervention group. The femurs were divided as follows: C group (no exercise without MSC injection); E group (exercise without MSC injection); M group (no exercise with MSC injection); and ME group (exercise with MSC injection). At 2, 4, and 8 weeks after the injection, the femurs were sectioned and histologically graded using the Wakitani cartilage repair scoring system. At 2 weeks after the injection, the total histological scores of the M and ME groups improved significantly compared with those of the C group. Four weeks after the injection, the scores of both the M and ME groups improved significantly. Additionally, the scores in the ME group showed a significant improvement compared to those in the M group. The improvement in the scores of the E, M, and ME groups at 8 weeks were not significantly different. The findings indicate that exercise may enhance cartilage repair after an MSC intra-articular injection. This study highlights the importance of exercise following cell transplantation therapy.

    DOI: 10.1371/journal.pone.0151580

    Web of Science

    PubMed

  59. Effect of microfabricated microgroove-surface devices on the morphology of mesenchymal stem cells. International journal

    Xiangkai Zhang, Tomoki Aoyama, Takashi Yasuda, Makoto Oike, Akira Ito, Junichi Tajino, Momoko Nagai, Rune Fujioka, Hirotaka Iijima, Shoki Yamaguchi, Norihiro Kakinuma, Hiroshi Kuroki

    Biomedical microdevices   Vol. 17 ( 6 ) page: 116 - 116   2015.12

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    The surface of a material that is in contact with cells is known to affect cell morphology and function. To develop an appropriate surface for tendon engineering, we used zigzag microgroove surfaces, which are similar to the tenocyte microenvironment. The purpose of this study was to investigate the effect of microgroove surfaces with different ridge angles (RAs), ridge lengths (RLs), ridge widths (RWs), and groove widths (GWs) on human bone marrow-derived mesenchymal stem cell (MSC) shape. Dishes with microgroove surfaces were fabricated using cyclic olefin polymer by injection-compression molding. The other parameters were fixed, and effects of different RAs (180 - 30 °), RLs (5 - 500 μm), RWs (5 - 500 μm), and GWs (5 - 500 μm) were examined. Changes in the zigzag shape of the cell due to different RAs, RLs, RWs, and GWs were observed by optical microscopy and scanning electron microscopy. Cytoskeletal changes were investigated using Phalloidin immunofluorescence staining. As observed by optical microscopy, MSCs changed to a zigzag shape in response to microgroove surfaces with different ridge and groove properties. . As observed by scanning electron microscopy, the cell shape changed at turns in the microgroove surface. Phalloidin immunofluorescence staining indicated that F-actin, not only in cell filopodia but also inside the cell body, changed orientation to conform to the microgrooves. In conclusion, the use of zigzag microgroove surfaces microfabricated by injection-compression molding demonstrated the property of MSCs to alter their shapes to fit the surface.

    DOI: 10.1007/s10544-015-0016-0

    PubMed

  60. Clinical Phenotype Classifications Based on Static Varus Alignment and Varus Thrust in Japanese Patients With Medial Knee Osteoarthritis. International journal

    Hirotaka Iijima, Naoto Fukutani, Tomoki Aoyama, Takahiko Fukumoto, Daisuke Uritani, Eishi Kaneda, Kazuo Ota, Hiroshi Kuroki, Shuichi Matsuda

    Arthritis & rheumatology (Hoboken, N.J.)   Vol. 67 ( 9 ) page: 2354 - 62   2015.9

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    OBJECTIVE: To investigate the association between knee pain during gait and 4 clinical phenotypes based on static varus alignment and varus thrust in patients with medial knee osteoarthritis (OA). METHODS: Patients in an orthopedic clinic (n = 266) diagnosed as having knee OA (Kellgren/Lawrence [K/L] grade ≥1) were divided into 4 phenotype groups according to the presence or absence of static varus alignment and varus thrust (dynamic varus): no varus (n = 173), dynamic varus (n = 17), static varus (n = 50), and static varus + dynamic varus (n = 26). The knee range of motion, spatiotemporal gait parameters, visual analog scale scores for knee pain, and scores on the Japanese Knee Osteoarthritis Measure were used to assess clinical outcomes. Multiple logistic regression analyses identified the relationship between knee pain during gait and the 4 phenotypes, adjusted for possible risk factors, including age, sex, body mass index, K/L grade, and gait velocity. RESULTS: Multiple logistic regression analysis showed that varus thrust without varus alignment was associated with knee pain during gait (odds ratio [OR] 3.30, 95% confidence interval [95% CI] 1.08-12.4), and that varus thrust combined with varus alignment was strongly associated with knee pain during gait (OR 17.1, 95% CI 3.19-320.0). Sensitivity analyses applying alternative cutoff values for defining static varus alignment showed comparable results. CONCLUSION: Varus thrust with or without static varus alignment was associated with the occurrence of knee pain during gait. Tailored interventions based on individual malalignment phenotypes may improve clinical outcomes in patients with knee OA.

    DOI: 10.1002/art.39224

    PubMed

  61. Effects of short-term gentle treadmill walking on subchondral bone in a rat model of instability-induced osteoarthritis. International journal

    H Iijima, T Aoyama, A Ito, S Yamaguchi, M Nagai, J Tajino, X Zhang, H Kuroki

    Osteoarthritis and cartilage   Vol. 23 ( 9 ) page: 1563 - 74   2015.9

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    OBJECTIVE: Subchondral bone cyst (SBC) growth, caused by osteoclast activity during early knee osteoarthritis (OA) pathogenesis, should be treated to prevent further progressions of OA. In the present study, we evaluated the effects of gentle treadmill walking on subchondral bone and cartilage changes in an experimental rat model of destabilized medial meniscus (DMM). METHOD: Twelve-week-old Wistar rats underwent DMM surgery in their right knee and sham surgery in their left knee and were assigned to either the sedentary group or walking group (n = 42/group). Animals in the walking group were subjected to treadmill exercise 2 days after surgery, which included walking for 12 m/min, 30 min/day, 5 days/week for 1, 2, and 4 week(s). Subchondral bone and cartilage changes were evaluated by micro-CT analysis, histological analysis, and biomechanical analysis. RESULTS: Treadmill walking had a tendency to suppress SBC growth, which was confirmed by micro-CT (P = 0.06) and positive staining for tartrate-resistant acid phosphatase (TRAP) activity for the osteoclast number per bone surface (P = 0.09) 4 weeks after surgery. These changes coincide with the prevention of cartilage degeneration as evaluated by the Osteoarthritis Research Society International (OARSI) score (P < 0.05) and biomechanically softening (P < 0.05). Furthermore, treadmill walking could suppressed increasing osteocyte deaths (P < 0.01), which was positively correlated with the OARSI score (r = 0.77; P < 0.01). CONCLUSION: These results indicate biomechanical and biological links exist between cartilage and subchondral bone; preventive effects of treadmill walking on subchondral bone deterioration might be partly explained by the chondroprotective effects.

    DOI: 10.1016/j.joca.2015.04.015

    PubMed

  62. The effects of short-term hypoxia on human mesenchymal stem cell proliferation, viability and p16(INK4A) mRNA expression: Investigation using a simple hypoxic culture system with a deoxidizing agent

    Ito Akira, Aoyama Tomoki, Yoshizawa Makoto, Nagai Momoko, Tajino Junichi, Yamaguchi Shoki, Iijima Hirotaka, Zhang Xiangkai, Kuroki Hiroshi

      Vol. 11 ( 1 ) page: 25 - 31   2015.5

     More details

    Language:English  

    A hypoxic environment is thought to be important for the maintenance of stemness and suppressing cell senescence, in stem cells. Therefore, a hypoxic condition is induced during cell expansion and/or induction of intended differentiation. However, the induction of these conditions requires a specially equipped hypoxia chamber and expensive gas mixtures, which are expensive and space-consuming. Owing to these restrictions, appropriate hypoxic conditions cannot be provided during cell transportation, which is increasingly required for regenerative medicine. Hence, a simple and economical culture system is required. The purpose of this study was to investigate the effects of short-term hypoxic conditions on human mesenchymal stem cell (MSC) proliferation, viability, and senescence, utilizing the CulturePal system (CulturePal-Zero and CulturePal-Five), a novel and simple hypoxic culture system with a built-in deoxidizing agent. The O₂ concentration in the CulturePal-Zero was observed to reduce to <0.1% within 1 h, and to 5% within 24 h in the CulturePal-Five system. Cell proliferation under these hypoxic conditions showed a sharp increase at 5% O₂ concentration, and no noticeable cell death was observed even at severe hypoxic conditions (<0.1% O₂) up to 72h. The p16INK4A (cell senescence marker) mRNA expression was retained under hypoxic conditions up to 72h, but it was up-regulated under normoxic conditions. Interestingly, the p16INK4A expression altered proportionately to the O₂ concentration. These results indicated that the short-term hypoxic condition, at an approximate O₂ concentration of 5%, would be suitable for promoting cell proliferation and repressing cell senescence, without aggravating the MSC viability. Therefore, the CulturePal systems may be suitable for providing an appropriate hypoxic condition in stem cell research and transportation.

    CiNii Research

  63. Alteration of cartilage surface collagen fibers differs locally after immobilization of knee joints in rats. International journal

    Momoko Nagai, Tomoki Aoyama, Akira Ito, Junichi Tajino, Hirotaka Iijima, Shoki Yamaguchi, Xiangkai Zhang, Hiroshi Kuroki

    Journal of anatomy   Vol. 226 ( 5 ) page: 447 - 57   2015.5

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    The purpose of this study was to examine the ultrastructural changes of surface cartilage collagen fibers, which differ by region and the length of the experimental period in an immobilization model of rat. Male Wistar rats were randomly divided into histological or macroscopic and ultrastructural assessment groups. The left knees of all the animals were surgically immobilized by external fixation for 1, 2, 4, 8 or 16 weeks (n = 5/time point). Sagittal histological sections of the medial mid-condylar region of the knee were obtained and assessed in four specific regions (contact and peripheral regions of the femur and tibia) and two zones (superficial and deep). To semi-quantify the staining intensity of the collagen fibers in the cartilage, picrosirius red staining was used. The cartilage surface changes of all the assessed regions were investigated by scanning electron microscopy (SEM). From histological and SEM observations, the fibrillation and irregular changes of the cartilage surface were more severe in the peripheral region than in the contact region. Interestingly, at 16 weeks post-immobilization, we observed non-fibrous structures at both the contact and peripheral regions. The collagen fiber staining intensity decreased in the contact region compared with the peripheral region. In conclusion, the alteration of surface collagen fiber ultrastructure and collagen staining intensity differed by the specific cartilage regions after immobilization. These results demonstrate that the progressive degeneration of cartilage is region specific, and depends on the length of the immobilization period.

    DOI: 10.1111/joa.12290

    PubMed

  64. Culture temperature affects redifferentiation and cartilaginous extracellular matrix formation in dedifferentiated human chondrocytes. International journal

    Akira Ito, Tomoki Aoyama, Hirotaka Iijima, Junichi Tajino, Momoko Nagai, Shoki Yamaguchi, Xiangkai Zhang, Hiroshi Kuroki

    Journal of orthopaedic research : official publication of the Orthopaedic Research Society   Vol. 33 ( 5 ) page: 633 - 9   2015.5

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    To date, there have been few studies on how temperature affects the phenotype and metabolism of human chondrocytes. Thus, the purpose of this study was to elucidate the effects of culture temperature on chondrocyte redifferentiation and extracellular matrix (ECM) formation using dedifferentiated mature human chondrocytes in vitro. Dedifferentiated chondrocytes were cultured in a pellet culture system for up to 21 days. The pellets were randomly divided into three groups with different culture temperature (32, 37, and 41°C). Chondrocyte redifferentiation and ECM formation were evaluated by wet weight, messenger ribonucleic acid (mRNA), histological, and biochemical analyses. The results showed that the wet weight and the mRNA expressions of collagen type II A1 and cartilage oligomeric matrix protein at 37°C were higher than the corresponding values at 32°C. The histological and biochemical analyses revealed that the syntheses of type II collagen and proteoglycan were promoted at 37°C compared to those at 32°C, whereas they were considerably inhibited at 41°C. In conclusion, the results obtained herein indicated that temperature affects chondrocyte redifferentiation and ECM formation, and modulation of temperature might thus represent an advantageous means to regulate the phenotype and biosynthetic activity of chondrocytes.

    DOI: 10.1002/jor.22808

    PubMed

  65. The effects of short-term hypoxia on human mesenchymal stem cell proliferation, viability and p16<sup>INK4A</sup> mRNA expression: Investigation using a simple hypoxic culture system with a deoxidizing agent

    Ito A., Aoyama T., Yoshizawa M., Nagai M., Tajino J., Yamaguchi S., Iijima H., Zhang X., Kuroki H.

    Journal of Stem Cells and Regenerative Medicine   Vol. 11 ( 1 ) page: P25 - P31   2015

     More details

    Language:Japanese   Publisher:Journal of Stem Cells and Regenerative Medicine  

    A hypoxic environment is thought to be important for the maintenance of stemness and suppressing cell senescence, in stem cells. Therefore, a hypoxic condition is induced during cell expansion and/or induction of intended differentiation. However, the induction of these conditions requires a specially equipped hypoxia chamber and expensive gas mixtures, which are expensive and space-consuming. Owing to these restrictions, appropriate hypoxic conditions cannot be provided during cell transportation, which is increasingly required for regenerative medicine. Hence, a simple and economical culture system is required. The purpose of this study was to investigate the effects of short-term hypoxic conditions on human mesenchymal stem cell (MSC) proliferation, viability, and senescence, utilizing the CulturePal system (CulturePal-Zero and CulturePal-Five), a novel and simple hypoxic culture system with a built-in deoxidizing agent. The O2 concentration in the CulturePal-Zero was observed to reduce to <0.1% within 1 h, and to 5% within 24h in the CulturePal-Five system. Cell proliferation under these hypoxic conditions showed a sharp increase at 5% O2 concentration, and no noticeable cell death was observed even at severe hypoxic conditions (<0.1% O2) up to 72h. The p16INK4A (cell senescence marker) mRNA expression was retained under hypoxic conditions up to 72h, but it was up-regulated under normoxic conditions. Interestingly, the p16INK4A expression altered proportionately to the O2 concentration. These results indicated that the short-term hypoxic condition, at an approximate O2 concentration of 5%, would be suitable for promoting cell proliferation and repressing cell senescence, without aggravating the MSC viability. Therefore, the CulturePal systems may be suitable for providing an appropriate hypoxic condition in stem cell research and transportation.

    Scopus

  66. Culture temperature affects human chondrocyte messenger RNA expression in monolayer and pellet culture systems. International journal

    Akira Ito, Momoko Nagai, Junichi Tajino, Shoki Yamaguchi, Hirotaka Iijima, Xiangkai Zhang, Tomoki Aoyama, Hiroshi Kuroki

    PloS one   Vol. 10 ( 5 ) page: e0128082   2015

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    Cell-based therapy has been explored for articular cartilage regeneration. Autologous chondrocyte implantation is a promising cell-based technique for repairing articular cartilage defects. However, there are several issues such as chondrocyte de-differentiation. While numerous studies have been designed to overcome some of these issues, only a few have focused on the thermal environment that can affect chondrocyte metabolism and phenotype. In this study, the effects of different culture temperatures on human chondrocyte metabolism- and phenotype-related gene expression were investigated in 2D and 3D environments. Human chondrocytes were cultured in a monolayer or in a pellet culture system at three different culture temperatures (32°C, 37°C, and 41°C) for 3 days. The results showed that the total RNA level, normalized to the threshold cycle value of internal reference genes, was higher at lower temperatures in both culture systems. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and citrate synthase (CS), which are involved in glycolysis and the citric acid cycle, respectively, were expressed at similar levels at 32°C and 37°C in pellet cultures, but the levels were significantly lower at 41°C. Expression of the chondrogenic markers, collagen type IIA1 (COL2A1) and aggrecan (ACAN), was higher at 37°C than at 32°C and 41°C in both culture systems. However, this phenomenon did not coincide with SRY (sex-determining region Y)-box 9 (SOX9), which is a fundamental transcription factor for chondrogenesis, indicating that a SOX9-independent pathway might be involved in this phenomenon. In conclusion, the expression of chondrocyte metabolism-related genes at 32°C was maintained or enhanced compared to that at 37°C. However, chondrogenesis-related genes were further induced at 37°C in both culture systems. Therefore, manipulating the culture temperature may be an advantageous approach for regulating human chondrocyte metabolic activity and chondrogenesis.

    DOI: 10.1371/journal.pone.0128082

    PubMed

  67. The effects of short-term hypoxia on human mesenchymal stem cell proliferation, viability and p16(INK4A) mRNA expression: Investigation using a simple hypoxic culture system with a deoxidizing agent. International journal

    Akira Ito, Tomoki Aoyama, Makoto Yoshizawa, Momoko Nagai, Junichi Tajino, Shoki Yamaguchi, Hirotaka Iijima, Xiangkai Zhang, Hiroshi Kuroki

    Journal of stem cells & regenerative medicine   Vol. 11 ( 1 ) page: 25 - 31   2015

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    A hypoxic environment is thought to be important for the maintenance of stemness and suppressing cell senescence, in stem cells. Therefore, a hypoxic condition is induced during cell expansion and/or induction of intended differentiation. However, the induction of these conditions requires a specially equipped hypoxia chamber and expensive gas mixtures, which are expensive and space-consuming. Owing to these restrictions, appropriate hypoxic conditions cannot be provided during cell transportation, which is increasingly required for regenerative medicine. Hence, a simple and economical culture system is required. The purpose of this study was to investigate the effects of short-term hypoxic conditions on human mesenchymal stem cell (MSC) proliferation, viability, and senescence, utilizing the CulturePal system (CulturePal-Zero and CulturePal-Five), a novel and simple hypoxic culture system with a built-in deoxidizing agent. The O2 concentration in the CulturePal-Zero was observed to reduce to <0.1% within 1 h, and to 5% within 24h in the CulturePal-Five system. Cell proliferation under these hypoxic conditions showed a sharp increase at 5% O2 concentration, and no noticeable cell death was observed even at severe hypoxic conditions (<0.1% O2) up to 72h. The p16(INK4A) (cell senescence marker) mRNA expression was retained under hypoxic conditions up to 72h, but it was up-regulated under normoxic conditions. Interestingly, the p16(INK4A) expression altered proportionately to the O2 concentration. These results indicated that the short-term hypoxic condition, at an approximate O2 concentration of 5%, would be suitable for promoting cell proliferation and repressing cell senescence, without aggravating the MSC viability. Therefore, the CulturePal systems may be suitable for providing an appropriate hypoxic condition in stem cell research and transportation.

    PubMed

  68. Intermittent application of hypergravity by centrifugation attenuates disruption of rat gait induced by 2 weeks of simulated microgravity. International journal

    Junichi Tajino, Akira Ito, Momoko Nagai, Xiangkai Zhang, Shoki Yamaguchi, Hirotaka Iijima, Tomoki Aoyama, Hiroshi Kuroki

    Behavioural brain research   Vol. 287   page: 276 - 84   2015

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    The effects of intermittent hypergravity on gait alterations and hindlimb muscle atrophy in rats induced by 2 weeks of simulated microgravity were investigated. Rats were submitted to hindlimb unloading for 2 weeks (unloading period), followed by 2 weeks of reloading (recovery period). During the unloading period, animals were subjected to the following treatments: (1) free in cages (Control); (2) continuous unloading (UL); (3) released from unloading for 1 hour per day (UL+1G); (4) hypergravity for 1h per day using a centrifuge for small animals (UL+2G). The relative weights of muscles to the whole body weight and kinematics properties of hindlimbs during gait were evaluated. UL rats walked with their hindlimbs overextended, and the oscillation of their limb motion had become narrowed and forward-shifted after the unloading period, and this persisted for at least 2 weeks after the termination of unloading. However, these locomotor alterations were attenuated in rats subjected to UL+2G centrifugation despite minor systematic changes in muscle recovery. These findings indicate hypergravity application could counteract the adverse effects of simulated or actual microgravity environments.

    DOI: 10.1016/j.bbr.2015.03.030

    PubMed

  69. Discordance in recovery between altered locomotion and muscle atrophy induced by simulated microgravity in rats. International journal

    Junichi Tajino, Akira Ito, Momoko Nagai, Xiangkai Zhang, Shoki Yamaguchi, Hirotaka Iijima, Tomoki Aoyama, Hiroshi Kuroki

    Journal of motor behavior   Vol. 47 ( 5 ) page: 397 - 406   2015

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    Exposure to a microgravity environment leads to adverse effects in motion and musculoskeletal properties. However, few studies have investigated the recovery of altered locomotion and muscle atrophy simultaneously. The authors investigated altered locomotion in rats submitted to simulated microgravity by hindlimb unloading for 2 weeks. Motion deficits were characterized by hyperextension of the knees and ankle joints and forward-shifted limb motion. Furthermore, these locomotor deficits did not revert to their original form after a 2-week recovery period, although muscle atrophy in the hindlimbs had recovered, implying discordance in recovery between altered locomotion and muscle atrophy, and that other factors such as neural drives might control behavioral adaptations to microgravity.

    DOI: 10.1080/00222895.2014.1003779

    PubMed

  70. Regional comparisons of porcine menisci. International journal

    Xiangkai Zhang, Tomoki Aoyama, Akira Ito, Junichi Tajino, Momoko Nagai, Shoki Yamaguchi, Hirotaka Iijima, Hiroshi Kuroki

    Journal of orthopaedic research : official publication of the Orthopaedic Research Society   Vol. 32 ( 12 ) page: 1602 - 11   2014.12

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    The purpose of this study was to analyze histologic, biochemical, and biomechanical differences between zonal, regional, and anatomic locations of porcine menisci. We evaluated six menisci removed from pigs. Medial and lateral menisci were divided into three regions: anterior, middle, and posterior. In each portion, the central zone (CZ) and peripheral zone (PZ) were examined histologically (hematoxylin & eosin, safranin O/Fast green, and picrosiriusred staining), using scanning electron microscopy, biochemically (hydroxyproline assay for collagen content and dimethylmethylene blue assay for glycosaminoglycan [GAG] content), and biomechanically (compression testing). Collagen content in the CZ was lower than that in the PZ. GAG content in the CZ was higher than that in the PZ. GAG content in the PZ of the posterior portion was significantly higher than that in the anterior and middle portions. Compression strength in the CZ was higher than that in the PZ. The differences in cellular phenotype, vascular penetration, and ECM not only between CZ and PZ but also among the anterior, middle, and posterior portions were clarified in the immature porcine meniscus. This result helps further our understanding of the biological characteristic of the meniscus. © 2014 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 32:1602-1611, 2014.

    DOI: 10.1002/jor.22687

    Web of Science

    Scopus

    PubMed

  71. Contributions of biarticular myogenic components to the limitation of the range of motion after immobilization of rat knee joint. International journal

    Momoko Nagai, Tomoki Aoyama, Akira Ito, Hirotaka Iijima, Shoki Yamaguchi, Junichi Tajino, Xiangkai Zhang, Haruhiko Akiyama, Hiroshi Kuroki

    BMC musculoskeletal disorders   Vol. 15   page: 224 - 224   2014.7

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    BACKGROUND: Muscle atrophy caused by immobilization in the shortened position is characterized by a decrease in the size or cross-sectional area (CSA) of myofibers and decreased muscle length. Few studies have addressed the relationship between limitation of the range of motion (ROM) and the changes in CSA specifically in biarticular muscles after atrophy because of immobilization. We aimed to determine the contribution of 2 distinct muscle groups, the biarticular muscles of the post thigh (PT) and those of the post leg (PL), to the limitation of ROM as well as changes in the myofiber CSAs after joint immobilization surgery. METHODS: Male Wistar rats (n = 40) were randomly divided into experimental and control groups. In the experimental group, the left knee was surgically immobilized by external fixation for 1, 2, 4, 8, or 16 weeks (n = 5 each) and sham surgery was performed on the right knee. The rats in the control groups (n = 3 per time point) did not undergo surgery. After the indicated immobilization periods, myotomy of the PT or PL biarticular muscles was performed and the ROM was measured. The hamstrings and gastrocnemius muscles from the animals operated for 1 or 16 weeks were subjected to morphological analysis. RESULTS: In immobilized knees, the relative contribution of the PT biarticular myogenic components to the total restriction reached 80% throughout the first 4 weeks and decreased thereafter. The relative contribution of the PL biarticular myogenic components remained <20% throughout the immobilization period. The ratio of the myofiber CSA of the immobilized to that of the sham-operated knees was significantly lower at 16 weeks after surgery than at 1 week after surgery only in the hamstrings. CONCLUSIONS: The relative contribution of the PT and PL components to myogenic contracture did not significantly change during the experimental period. However, the ratio of hamstrings CSAs to the sham side was larger than the ratio of medial gastrocnemius CSAs to the sham side after complete atrophy because of immobilization.

    DOI: 10.1186/1471-2474-15-224

    PubMed

  72. Destabilization of the medial meniscus leads to subchondral bone defects and site-specific cartilage degeneration in an experimental rat model. International journal

    H Iijima, T Aoyama, A Ito, J Tajino, M Nagai, X Zhang, S Yamaguchi, H Akiyama, H Kuroki

    Osteoarthritis and cartilage   Vol. 22 ( 7 ) page: 1036 - 43   2014.7

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    OBJECTIVE: This study aimed to investigate subchondral bone changes using micro-computed tomography (micro-CT) and regional differences in articular cartilage degeneration, focusing on changes of cartilage covered by menisci, in the early phase using a destabilization of the medial meniscus (DMM) model. METHOD: The DMM model was created as an experimental rat osteoarthritis (OA) model (12 weeks old; n = 24). At 1, 2, and 4 weeks after surgery, the rats were sacrificed, and knee joints were scanned using a Micro-CT system. Histological sections of the medial tibial plateau, which was divided into inner, middle, and outer regions, were prepared and scored using the modified OARSI scoring system. The cartilage thickness was also calculated, and matrix metalloproteinase 13 (MMP13), Col2-3/4c, and vascular endothelial growth factor (VEGF) expression was assessed immunohistochemically. RESULTS: Subchondral bone defects were observed in the middle region, in which the cartilage thickness decreased over time after surgery, and these defects were filled with MMP13- and VEGF-expressing fibrous tissue. The OARSI score increased over time in the middle region, and the score was significantly higher in the middle region than in the inner and outer regions at 1, 2, and 4 weeks after surgery. Col2-3/4c and MMP13 expression was observed primarily in the meniscus-covered outer region, in which the cartilage thickness increased over time. CONCLUSION: Loss of meniscal function caused cartilage degeneration and subchondral bone defects in the early phase site-specifically in the middle region. Furthermore, our results might indicate cartilage covered by menisci is easily degraded resulting in osmotic swelling of the cartilage in early OA.

    DOI: 10.1016/j.joca.2014.05.009

    PubMed

  73. Evaluation of reference genes for human chondrocytes cultured in several different thermal environments. International journal

    Akira Ito, Tomoki Aoyama, Junichi Tajino, Momoko Nagai, Shoki Yamaguchi, Hirotaka Iijima, Xiangkai Zhang, Haruhiko Akiyama, Hiroshi Kuroki

    International journal of hyperthermia : the official journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group   Vol. 30 ( 3 ) page: 210 - 6   2014.5

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    PURPOSE: The purpose of this study was to identify reference genes showing stable expression in chondrocytes cultured under several different thermal environments and in different culture systems. MATERIALS AND METHODS: Human articular chondrocytes were cultured by monolayer or pellet culture system at 32 °C, 37 °C, and 41 °C for 3 days. Thereafter, the total RNA was extracted, and quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR) was performed. The qRT-PCR data was analysed using three different algorithms (geNorm, NormFinder, and BestKeeper) to identify reference genes exhibiting stable expression from among the seven candidate reference genes (B2M, ACTB, GAPDH, HSPCB, RPL13a, YWHAZ, and 18S). RESULTS: The candidate reference genes, except for HSPCB and YWHAZ, showed systematic variations in expression. In the monolayer culture, RPL13a was the most stable gene identified using NormFinder and BestKeeper; on using geNorm, ACTB and GAPDH showed the highest expression stability. In the pellet culture, ACTB was the most stable gene identified using NormFinder and BestKeeper, whereas GAPDH and RPL13a were the most stable reference genes as determined using geNorm. In the combined group, B2M and GAPDH were the most stable genes identified using geNorm, whereas RPL13a and YWHAZ were the most stable as per NormFinder and BestKeeper, respectively. The best combination of two candidate reference genes among all the groups determined using NormFinder was RPL13a and YWHAZ. CONCLUSION: The combination of RPL13a and YWHAZ might be suitable as reference genes for human chondrocytes cultured at 32 °C, 37 °C, and 41 °C in monolayer, pellet, or combined cultures.

    DOI: 10.3109/02656736.2014.906048

    PubMed

  74. Immature articular cartilage and subchondral bone covered by menisci are potentially susceptive to mechanical load. International journal

    Hirotaka Iijima, Tomoki Aoyama, Akira Ito, Junichi Tajino, Momoko Nagai, Xiangkai Zhang, Shoki Yamaguchi, Haruhiko Akiyama, Hiroshi Kuroki

    BMC musculoskeletal disorders   Vol. 15   page: 101 - 101   2014.3

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    BACKGROUND: The differences of mechanical and histological properties between cartilage covered by menisci and uncovered by menisci may contribute to the osteoarthritis after meniscectomy and these differences are not fully understood. The purpose of this study is to investigate potential differences in the mechanical and histological properties, and in particular the collagen architecture, of the superficial cartilage layer and subchondral bone between regions covered and uncovered by menisci using immature knee. METHODS: Osteochondral plugs were obtained from porcine tibial cartilage that was either covered or uncovered by menisci. Investigation of the thickness, mechanical properties, histology, and water content of the cartilage as well as micro-computed tomography analysis of the subchondral bone was performed to compare these regions. Collagen architecture was also assessed by using scanning electron microscopy. RESULTS: Compared to the cartilage uncovered by menisci, that covered by menisci was thinner and showed a higher deformity to compression loading and higher water content. In the superficial layer of cartilage in the uncovered regions, collagen fibers showed high density, whereas they showed low density in covered regions. Furthermore, subchondral bone architecture varied between the 2 regions, and showed low bone density in covered regions. CONCLUSIONS: Cartilage covered by menisci differed from that uncovered in both its mechanical and histological properties, especially with regards to the density of the superficial collagen layer. These regional differences may be related to local mechanical environment in normal condition and indicate that cartilage covered by menisci is tightly guarded by menisci from extreme mechanical loading. Our results indicate that immature cartilage degeneration and subchondral microfracture may occur easily to extreme direct mechanical loading in covered region after meniscectomy.

    DOI: 10.1186/1471-2474-15-101

    PubMed

  75. Optimum temperature for extracellular matrix production by articular chondrocytes. International journal

    Akira Ito, Tomoki Aoyama, Hirotaka Iijima, Momoko Nagai, Shoki Yamaguchi, Junichi Tajino, Xiangkai Zhang, Haruhiko Akiyama, Hiroshi Kuroki

    International journal of hyperthermia : the official journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group   Vol. 30 ( 2 ) page: 96 - 101   2014.3

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    PURPOSE: The purpose of this study was to investigate the influence of temperature on the ability of the chondrocytes to produce extracellular matrix (ECM). MATERIALS AND METHODS: Articular chondrocytes were isolated from porcine knee joints. The chondrocytes were cultured at three different temperatures: 32 °C, 37 °C, and 41 °C. The ability to produce ECM was assessed by gene expression analysis, histological, and biochemical evaluation in a pellet culture system. RESULTS: Wet weight of the pellets generated after 21 days, was significantly heavier when cultured at lower temperatures. Picrosirius red staining, employed to evaluate collagen production, was higher at lower temperatures, and safranin-O staining, used to evaluate sulphated glycosaminoglycan (GAG), was lower at 32 °C than at 37 °C and 41 °C. Collagen type IIA1 mRNA expression was markedly up-regulated at 41 °C. However, picrosirius red staining was inhibited at 41 °C. GAG and DNA content were measured by 1,9-dimethylmethylene blue (DMMB) assay and PicoGreen® assay, respectively. The GAG content per pellet was significantly low at 41 °C compared to that at 32 °C and 37 °C. The DNA content per pellet was larger at lower temperatures. The GAG content normalised with the DNA content per pellet was significantly lower at 32 °C compared to that at 37 °C and 41 °C. CONCLUSION: Our results suggest that a culture temperature of approximately 41 °C inhibits ECM production by decreasing DNA content and perhaps by collagen misfolding. Taken together, the optimum temperature for ECM production in articular chondrocytes may be between 32 °C and 37 °C.

    DOI: 10.3109/02656736.2014.880857

    PubMed

  76. Effects of knee orthoses on walking capacity and biomechanics in patients with knee osteoarthritis: A critical review

    Hirotaka Iijima, Takuya Isho, Tomoki Aoyama

    OA Musculosk Med   Vol. 2 ( 13 )   2014

     More details

    Authorship:Lead author  

  77. Effects of the thermal environment on articular chondrocyte metabolism: a fundamental study to facilitate establishment of an effective thermotherapy for osteoarthritis.

    Akira Ito, Tomoki Aoyama, Junichi Tajino, Momoko Nagai, Shoki Yamaguchi, Hirotaka Iijima, Xiangkai Zhang, Haruhiko Akiyama, Hiroshi Kuroki

    Journal of the Japanese Physical Therapy Association = Rigaku ryoho   Vol. 17 ( 1 ) page: 14 - 21   2014

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    AIM: To facilitate establishment of an effective thermotherapy for osteoarthritis (OA), we investigated the effects of the thermal environment on articular chondrocyte metabolism in vitro. METHODS: Chondrocytes were isolated from porcine knee joints, and cultured at 32°C, 37°C and 41°C. Cell proliferation and viability were assessed at Days 2, 4 and 8. In addition, TdT-mediated dUTP nick end labeling (TUNEL) assay was performed at Day 3 to determine the proportion of apoptotic chondrocytes. Analysis of genes specific for factors related to the cartilage extracellular matrix (ECM), cartilage destruction, and cartilage protection was performed at Day 2. Furthermore, evaluation of heat stress tolerance, and heat shock protein 70 (HSP70) mRNA expression and protein synthesis was performed at Day 2 and 3, respectively. RESULTS: Cell proliferation was more at 37°C than at 32°C and 41°C. Cell viability and the number of TUNEL-positive cells were not affected until Day 8 and 3, respectively. The expression of the ECM-related genes was up-regulated at higher temperature. The expression of MMP13, a type II collagen destructive enzyme, and that of TIMP1 and TIMP2, which are MMP inhibitors, were up-regulated at higher temperatures. Finally, the chondrocytes cultured at 41°C may acquire heat stress tolerance, in part, due to the up-regulation of HSP70, and may inhibit apoptosis induced by various stresses, which is observed in OA. CONCLUSIONS: The thermal environment affects articular chondrocyte metabolism, and a heat stimulus of approximately 41°C could enhance chondrocyte anabolism and induce heat stress tolerance.

    DOI: 10.1298/jjpta.Vol17_003

    PubMed

  78. Mechanical and Histological Properties of Articular Cartilage that Load Through Menisci: Experiment Study Using Porcine Knee Reviewed

    飯島弘貴, 青山朋樹, 伊藤明良, 太治野純一, 長井桃子, ZHANG Xiangkai, 山口将希, 秋山治彦, 黒木裕士

    理学療法学   Vol. 40 ( 5 ) page: 355 - 363   2013.8

  79. Effects of exercise level on biomarkers in a rat knee model of osteoarthritis. International journal

    Shoki Yamaguchi, Tomoki Aoyama, Akira Ito, Momoko Nagai, Hirotaka Iijima, Xiangkai Zhang, Junichi Tajino, Hiroshi Kuroki

    Journal of orthopaedic research : official publication of the Orthopaedic Research Society   Vol. 31 ( 7 ) page: 1026 - 31   2013.7

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    The aim of this study was to elucidate whether the levels of serum biomarkers reflect the progression of osteoarthritis (OA) induced by different levels of exercise. Thirty-five Wistar rats subjected to anterior cruciate ligament transaction (ACLT) were divided into three groups: Control, moderate running, and intense running. Twelve rats (moderate running without ACLT) were allocated as a naive group. Running was performed on a motorized treadmill, at a speed of 18 m/min for 30 min/day (moderate and naive) or 60 min/day (intense) for 3 days per week. After 2 or 4 weeks, OA histopathology in the knees was evaluated using the Osteoarthritis Research Society International (OARSI) score, and the serum levels of cleaved collagen type II (C2C) and procollagen II C-propeptide (CPII) were analyzed. The OARSI score deteriorated in the intense running group after 2 weeks and the serum C2C/CPII ratio suggested the development of OA. At 4 weeks, the C2C/CPII ratio suggested there would be deterioration in the OARSI score but the score did not differ significantly between the moderate and intense running groups. C2C/CPII ratio had 13-25% correlation with the OARSI histological score. Thus, in rat experimental OA, the OARSI score could be partially predicted by the C2C/CPII ratio as a serum biomarker of OA.

    DOI: 10.1002/jor.22332

    PubMed

  80. Mechanical and Historogical Properties of Articular Cartilage that Load Through Menisci :

    Iijima Hirotaka, Aoyama Tomoki, Ito Akira, Tajino Junichi, Nagai Momoko, Zhang Xiangkai, Yamaguchi Shoki, Akiyama Haruhiko, Kuroki Hiroshi

    Physical Therapy Japan   Vol. 40 ( 5 ) page: 355 - 363   2013

     More details

    Language:Japanese   Publisher:Japanese Society of Physical Therapy  

    Purpose: To investigate differences in the mechanical and histological properties and in the collagen architecture, particularly the superficial layer, between the regions covered by menisci and those not covered by menisci. Methods: Osteochondral plugs were obtained from porcine tibial cartilage that was either covered or not covered by menisci. Plugs obtained from each region were subjected to mechanical analysis by the indentation method, histological analysis by safranin O staining, and analysis of collagen fiber ultrastructure by scanning electron microscopy. Results: The axial creep was larger in cartilage covered by menisci than in cartilage not covered by menisci. Safranin O staining revealed a low proteoglycan content in cartilage covered by menisci than that not covered by menisci. The superficial layer of the collagen was less dense in cartilage covered by menisci than in that not covered by menisci. Conclusions: The results of our study confirmed that cartilage covered by menisci that load through menisci has a low proteoglycan content. Thus, cartilage covered by menisci may be deformed by loading after meniscal injury or meniscectomy to a greater extent than that not covered by menisci.

    DOI: 10.15063/rigaku.kj00008826560

    CiNii Research

  81. ラット足関節軟骨の加齢と運動の影響 Reviewed

    村田 健児, 金村 尚彦, 羽田 侑里子, 飯島 弘貴, 高柳 清美, 森山 英樹

    理学療法-臨床・研究・教育   Vol. 18   page: 61 - 66   2011.3

     More details

    Language:Japanese   Publishing type:Research paper (scientific journal)  

  82. 運動器障害に対する牽引療法の効果 Reviewed

    森山 英樹, 飯島 弘貴, 金村 尚彦, 羽田 侑里子, 村田 健児, 今北 英高, 高柳 清美, 伊藤 俊一, 五味 敏昭, 飛松 好子

    運動療法と物理療法   Vol. 20 ( 4 ) page: 369 - 378   2009.12

     More details

    Language:Japanese   Publishing type:Research paper (scientific journal)  

  83. 運動器障害に対する温熱療法の効果 Reviewed

    森山 英樹, 飯島 弘貴, 金村 尚彦, 村田 健児, 羽田 侑里子, 西原 賢, 小澤 淳也, 高柳 清美, 五味 敏昭, 飛松 好子

    運動療法と物理療法   Vol. 20 ( 3 ) page: 260 - 268   2009.11

     More details

    Language:Japanese   Publishing type:Research paper (scientific journal)  

▼display all

Books 1

  1. サプリメントの機能性:運動器・スポーツ

    青山朋樹, 飯島弘貴, 松田秀一( Role: Contributor ,  アンチエイジング医学の基礎と臨床. pp281–283)

    メジカルビュー社  2015 

MISC 5

  1. 変形性膝関節症におけるメカニカルストレス研究と間葉系幹細胞治療後の再生リハビリテーション

    飯島 弘貴

    日本基礎理学療法学雑誌   Vol. 22 ( 1 ) page: 1 - 8   2019.12

     More details

    Language:Japanese   Publisher:(一社)日本基礎理学療法学会  

    変形性膝関節症(膝OA)軟骨におけるメカニカルストレス研究について概説し、エクササイズによる強度依存的な関節軟骨保護・破壊作用やその分子メカニズムについて述べた。次いで、間葉系幹細胞を用いた膝OA軟骨再生治療におけるリハビリテーションの可能性について述べた。最後に、膝OAの再生リハビリテーション確立に向けた新しい運動機能評価システムを紹介した。

    Other Link: https://search-tp.jamas.or.jp/index.php?module=Default&action=Link&pub_year=2019&ichushi_jid=J05805&link_issn=&doc_id=20191227260001&doc_link_id=%2Fch1thrpy%2F2019%2F002201%2F001%2F0001-0008%26dl%3D0&url=https%3A%2F%2Fwww.medicalonline.jp%2Fjamas.php%3FGoodsID%3D%2Fch1thrpy%2F2019%2F002201%2F001%2F0001-0008%26dl%3D0&type=MedicalOnline&icon=https%3A%2F%2Fjk04.jamas.or.jp%2Ficon%2F00004_2.gif

  2. 【運動ってなんだ?"Exercise is Medicine"を支えるサイエンスを拓く】変形性膝関節症軟骨におけるメカニカルストレス研究 運動の強度依存的な軟骨保護・破壊作用とその分子メカニズム探求の挑戦 Invited

    飯島 弘貴

    実験医学   Vol. 37 ( 8 ) page: 1240 - 1245   2019.5

     More details

    Authorship:Lead author   Language:Japanese  

  3. 内側型変形性膝関節症患者における膝蓋大腿関節症合併が与える臨床的影響 Invited

    飯島 弘貴

    整形外科   Vol. 68 ( 12 ) page: 1317 - 1319   2017.11

     More details

    Authorship:Lead author  

  4. 【変形性膝関節症の診断と治療】保存的治療 理学療法 装具療法 変形性膝関節症に対する膝装具療法 膝関節痛・歩行バイオメカニクス・歩行能力に与える効果に関する臨床介入研究 Invited

    飯島 弘貴, 井所 拓哉, 青山 朋樹, 松田 秀一

    別冊整形外科   Vol. 67   page: 115 - 120   2015.4

     More details

    Authorship:Lead author  

  5. 変形性関節症に対する効果的なユビキタス治療法の開発 Invited

    黒木裕士, 青山朋樹, 長井桃子, 山口将希, 飯島弘貴, 伊藤明良

    大阪ガスグループ福祉財団調査・研究報告集   Vol. 27   2014

Presentations 124

  1. laser-TUGシステムを用いた頸髄症患者の歩行特性の抽出

    小山 恭史, 藤田 浩二, 野呂瀬 美生, 佐々木 亨, 鏑木 秀俊, 二村 昭元, 飯島 弘貴, 高橋 正樹, 大川 淳

    日本整形外科学会雑誌  2021.8  (公社)日本整形外科学会

     More details

    Event date: 2021.8

    Language:Japanese  

  2. 変形性膝関節症患者における痛みの破局的思考は階段昇降動作を困難にする

    鈴木祐介, 飯島弘貴, 飯島弘貴, 青山朋樹

    Japanese Journal of Rehabilitation Medicine  2020  (公社)日本リハビリテーション医学会

     More details

    Event date: 2020

    Language:Japanese  

  3. Laser-TUGシステムを用いた橈骨遠位端骨折患者の歩行状態の評価 転倒とドミノ骨折の予防を目指して

    藤田 浩二, 飯島 弘貴, 奥村 篤史, 八百 陽介, 鏑木 秀俊, 二村 昭元, 加藤 龍一, 大川 淳, 高橋 正樹

    日本手外科学会雑誌  2019.4  (一社)日本手外科学会

     More details

    Event date: 2019.4

    Language:Japanese  

  4. Laser Range Sensorを用いた大腿骨近位部骨折患者のTUG回転方向における時空間的パラメータの比較

    丸毛達也, 武田尊徳, 白滝智洋, 安原康平, 道下将矢, 吉野晃平, 飯島弘貴, 高橋正樹

    日本理学療法学術大会(Web)  2019  (公社)日本理学療法士協会

     More details

    Event date: 2019

    Language:Japanese  

  5. Mechanical stress research and regenerative rehabilitation after mesenchymal stem cell treatment in people with knee osteoarthritis

    飯島弘貴, 飯島弘貴, 飯島弘貴

    日本基礎理学療法学雑誌(Web)  2019 

     More details

    Event date: 2019

  6. 変形性膝関節症における立ち上がり動作の運動学的・運動力学的・筋電図的変化:システマティックレビュー

    園尾萌香, 園尾萌香, 飯島弘貴, 飯島弘貴, 金村尚彦

    日本理学療法学術大会(Web)  2019  (公社)日本理学療法士協会

     More details

    Event date: 2019

    Language:Japanese  

  7. 変形性膝関節症における再生リハビリテーション確立に向けた分野横断型研究

    飯島弘貴, 飯島弘貴, 飯島弘貴

    日本理学療法学術大会(Web)  2019  (公社)日本理学療法士協会

     More details

    Event date: 2019

    Language:Japanese  

  8. 橈骨遠位端骨折患者の歩行状態の評価転倒とドミノ骨折の予防を目指して

    藤田浩二, 二村昭元, 飯島弘貴, 江口僚, 加藤龍一, 大川淳, 高橋正樹

    日本骨粗鬆症学会雑誌  2019  (一社)日本骨粗鬆症学会

     More details

    Event date: 2019

    Language:Japanese  

  9. 膝半月不安定化による膝蓋大腿関節軟骨損傷と歩行キネマティクスの関係

    中畑晶博, 飯島弘貴, 飯島弘貴, 伊藤明良, 王天舒, 中原峻, 張ジュエ, 久保奈央子, 河合秀紀, 青山朋樹, 黒木裕士

    日本理学療法学術大会(Web)  2019  (公社)日本理学療法士協会

     More details

    Event date: 2019

    Language:Japanese  

  10. 早期変形性膝関節症患者の身体機能・疼痛に対する経皮的末梢神経電気刺激(TENS)の効果 ランダム化比較試験による効果検証

    下浦 佳南子, 飯島 弘貴, 鈴木 祐介, 梶原 由布, Zeidan Hala, 辰巳 昌嵩, 中井 謙吾, 西田 裕一, 備藤 翼, 吉見 そよ香, 青山 朋樹

    日本物理療法学会学術大会抄録集  2018.10  (一社)日本物理療法学会

     More details

    Event date: 2018.10

    Language:Japanese  

  11. Laser Range Sensorによる変形性膝関節症の方向転換動作解析と股関節外転筋の特異的貢献

    飯島弘貴, 飯島弘貴, 飯島弘貴, 青山朋樹, 高橋正樹

    Japanese Journal of Rehabilitation Medicine  2018  (公社)日本リハビリテーション医学会

     More details

    Event date: 2018

    Language:Japanese  

  12. レーザーレンジセンサを用いた変形性膝関節症患者の歩行パラメータ解析-膝関節痛が機能的移動能力に与える影響-

    飯島弘貴, 飯島弘貴, 飯島弘貴, 萬礼応, 江口僚, 青山朋樹, 高橋正樹

    Dynamics & Design Conference (CD-ROM)  2018 

     More details

    Event date: 2018

  13. 変形性膝関節症を有する高齢者を対象とした,膝痛の性質と立ち上がり能力の違いによる身体機能の比較

    鈴木祐介, 飯島弘貴, 飯島弘貴, 飯島弘貴, 田代雄斗, 青山朋樹

    Japanese Journal of Rehabilitation Medicine  2018  (公社)日本リハビリテーション医学会

     More details

    Event date: 2018

    Language:Japanese  

  14. Inertial Measurement Unit-Based Dynamic Functional Diagnosis

    飯島弘貴, 飯島弘貴, 飯島弘貴, 江口僚, 青山朋樹, 高橋正樹

    日本機械学会シンポジウム:スポーツ工学・ヒューマンダイナミクス講演論文集(CD-ROM)  2018 

     More details

    Event date: 2018

  15. Inertial Measurement Unit-Based Gait Diagnosis in Subjects with Knee Osteoarthritis and Novel Treatment Concept “GAIT”

    飯島弘貴, 飯島弘貴, 飯島弘貴, 江口僚, 青山朋樹, 高橋正樹

    LIFE講演概要集(CD-ROM)  2018 

     More details

    Event date: 2018

  16. 変形性膝関節症に対する間葉系幹細胞治療におけるリハビリテーション システマティックレビュー

    飯島弘貴, 飯島弘貴, 井所拓哉, 長井桃子, 伊藤明良, 太治野純一, 中畑晶博, 喜屋武弥, 張ジュエ, 王天舒, 黒木裕士, 青山朋樹

    日本理学療法学術大会(Web)  2017  (公社)日本理学療法士協会

     More details

    Event date: 2017

    Language:Japanese  

  17. 変形性膝関節症に対する間葉系幹細胞治療におけるリハビリテーション-臨床介入研究のシステマティックレビュー-

    飯島弘貴, 飯島弘貴, 井所拓哉, 井所拓哉, 黒木裕士, 青山朋樹

    日本軟骨代謝学会プログラム・抄録集  2017 

     More details

    Event date: 2017

  18. 微小重力環境によるラットの行動・動作の変化を遠心重力を用いて抑制する

    太治野純一, 伊藤明良, 長井桃子, 張項凱, 山口将希, 山口将希, 飯島弘貴, 飯島弘貴, 喜屋武弥, 青山朋樹, 黒木裕士

    日本宇宙航空環境医学会大会プログラム・予稿集  2017 

     More details

    Event date: 2017

  19. 関節固定が変形性膝関節症の関節構成体に与える影響 関節症モデル動物を用いた実験研究(パイロットスタディ)

    飯島弘貴, 飯島弘貴, 長井桃子, 伊藤明良, 太治野純一, 中畑晶博, 喜屋武弥, 張ジュエ, 王天舒, 青山朋樹, 黒木裕士

    日本理学療法学術大会(Web)  2017  (公社)日本理学療法士協会

     More details

    Event date: 2017

    Language:Japanese  

  20. 膝関節可動域制限は広範囲関節症変化の引き金となりうる-関節症モデル動物を用いた実験研究-

    飯島弘貴, 飯島弘貴, 谷間桃子, 内山綺紗良, 伊藤明良, 太治野純一, 中畑晶博, 喜屋武弥, 張ジュエ, 季翔, 王天舒, 青山朋樹, 西谷江平, 高橋正樹, 高橋正樹, 黒木裕士

    日本整形外科学会雑誌  2017  (公社)日本整形外科学会

     More details

    Event date: 2017

    Language:Japanese  

  21. 環境温度がラット核心温度・外層温度および活動量に及ぼす影響

    伊藤明良, 太治野純一, 飯島弘貴, 長井桃子, 中畑晶博, 張ジュエ, 王天舒, 喜屋武弥, 青山朋樹, 黒木裕士

    日本理学療法学術大会(Web)  2017  (公社)日本理学療法士協会

     More details

    Event date: 2017

    Language:Japanese  

  22. 末梢神経損傷患者への幹細胞を用いた治療におけるリハビリテーション-システマティックレビューによる現状把握-

    長井桃子, 飯島弘貴, 伊藤明良, 太治野純一, 中畑晶博, 喜屋武弥, 張ジュエ, 王天舒, 青山朋樹, 黒木裕士

    日本理学療法学術大会(Web)  2017  (公社)日本理学療法士協会

     More details

    Event date: 2017

    Language:Japanese  

  23. ラット変形性膝関節症モデルに対する強度別の運動刺激が関節軟骨-軟骨下骨複合体に与える影響

    飯島弘貴, 飯島弘貴, 青山朋樹, 伊藤明良, 伊藤明良, 山口将希, 山口将希, 長井桃子, 太治野純一, 張項凱, 喜屋武弥, 黒木裕士

    日本理学療法学術大会(Web)  2016  (公社)日本理学療法士協会

     More details

    Event date: 2016

    Language:Japanese  

  24. 均質な関節軟骨領域を求めて-物理的刺激効果検証に用いる関節軟骨部位の検討-

    伊藤明良, 伊藤明良, 飯島弘貴, 飯島弘貴, 太治野純一, 長井桃子, 山口将希, 山口将希, 張項凱, 喜屋武弥, 青山朋樹, 黒木裕士

    日本理学療法学術大会(Web)  2016  (公社)日本理学療法士協会

     More details

    Event date: 2016

    Language:Japanese  

  25. 内側半月板損傷が膝蓋大腿関節へ及ぼす影響-ラット変形性膝関節症モデルを用いた実験研究-

    中畑晶博, 青山朋樹, 飯島弘貴, 伊藤明良, 山口将希, 長井桃子, 太治野純一, 喜屋武弥, 張ジュエ, 王天舒, 黒木裕士

    運動器リハビリテーション  2016  日本運動器科学会

     More details

    Event date: 2016

    Language:Japanese  

  26. 中等度レベルのトレッドミル運動は変形性膝関節症の進行を遅らせる-ラット変形性膝関節症モデルを用いた実験研究-

    飯島弘貴, 飯島弘貴, 青山朋樹, 伊藤明良, 山口将希, 長井桃子, 太治野純一, 喜屋武弥, 中畑晶博, 張ジュエ, 王天舒, 黒木裕士

    運動器リハビリテーション  2016  日本運動器科学会

     More details

    Event date: 2016

    Language:Japanese  

  27. ラット膝関節不動モデルに対する荷重と非荷重が軟骨硬度に及ぼす影響

    長井桃子, 飯島弘貴, 伊藤明良, 太治野純一, 山口将希, 張項凱, 喜屋武弥, 青山朋樹, 黒木裕士

    日本理学療法学術大会(Web)  2016  (公社)日本理学療法士協会

     More details

    Event date: 2016

    Language:Japanese  

  28. ラット膝関節8週間不動後の自由飼育で見られた嚢胞様軟骨変性像に関する報告

    長井桃子, 伊藤明良, 太治野純一, 山口将希, 飯島弘貴, 張項凱, 喜屋武弥, 青山朋樹, 黒木裕士, 山田重人, 山田重人

    日本解剖学会総会・全国学術集会講演プログラム・抄録集  2016 

     More details

    Event date: 2016

  29. ラット変形性膝関節症モデルに対する強度別の運動刺激が関節軟骨-軟骨下骨複合体に与える影響

    飯島弘貴, 飯島弘貴, 青山朋樹, 伊藤明良, 伊藤明良, 山口将希, 山口将希, 長井桃子, 太治野純一, ZHANG Xiangkai, 喜屋武弥, 黒木裕士

    日本軟骨代謝学会プログラム・抄録集  2016 

     More details

    Event date: 2016

  30. 変形性膝関節症患者における活動量と身体機能の関係性

    飯島弘貴, 福谷直人, 井所拓哉, 山本裕子, 平岡正和, 宮信和幸, 陳之内将志, 金田瑛司, 金田瑛司, 青山朋樹, 松田秀一, 黒木裕士

    日本理学療法学術大会(Web)  2016  (公社)日本理学療法士協会

     More details

    Event date: 2016

    Language:Japanese  

  31. 骨軟骨欠損モデルラットに対する細胞移植と低出力超音波パルス治療の併用が欠損修復に及ぼす影響

    山口将希, 山口将希, 青山朋樹, 伊藤明良, 伊藤明良, 飯島弘貴, 飯島弘貴, 長井桃子, 太治野純一, 張項凱, 黒木裕士

    日本理学療法学術大会(Web)  2016  (公社)日本理学療法士協会

     More details

    Event date: 2016

    Language:Japanese  

  32. 微小重力環境によるラットの活動量リズム変化を遠心重力を用いて抑制する

    太治野純一, 伊藤明良, 長井桃子, 張項凱, 山口将希, 山口将希, 飯島弘貴, 飯島弘貴, 喜屋武弥, 青山朋樹, 黒木裕士

    日本宇宙航空環境医学会大会プログラム・予稿集  2016  日本宇宙航空環境医学会

     More details

    Event date: 2016

    Language:Japanese  

  33. 後肢免荷に対する高重力介入がラットの歩容変化に与える影響

    太治野純一, 伊藤明良, 伊藤明良, 長井桃子, 張項凱, 山口将希, 山口将希, 飯島弘貴, 飯島弘貴, 喜屋武弥, 青山朋樹, 黒木裕士

    日本理学療法学術大会(Web)  2016  (公社)日本理学療法士協会

     More details

    Event date: 2016

    Language:Japanese  

  34. 8週間の関節不動後の自由飼育が関節軟骨に与える影響~嚢胞様軟骨変性像の形成過程に関する報告~

    長井桃子, 伊藤明良, 太治野純一, 山口将希, 飯島弘貴, 張項凱, 喜屋武弥, 青山朋樹, 黒木裕士

    日本理学療法学術大会(Web)  2015  (公社)日本理学療法士協会

     More details

    Event date: 2015

    Language:Japanese  

  35. レントゲン画像所見と歩行観察に基づく内側型変形性膝関節症サブグループ作成の試み

    飯島弘貴, 福谷直人, 青山朋樹, 山本裕子, 平岡正和, 宮信和幸, 陳之内将志, 金田瑛司, 松田秀一, 黒木裕士

    日本理学療法学術大会(Web)  2015  (公社)日本理学療法士協会

     More details

    Event date: 2015

    Language:Japanese  

  36. 半月板損傷モデルラットに対する軽度な運動負荷が関節軟骨,軟骨下骨に与える影響の検討

    飯島弘貴, 青山朋樹, 伊藤明良, 山口将希, 長井桃子, 太治野純一, ZHANG Xiangkai, 喜屋武弥, 黒木裕士

    日本軟骨代謝学会プログラム・抄録集  2015 

     More details

    Event date: 2015

  37. 低出力超音波パルスは骨軟骨欠損モデルラットの軟骨再生に影響を及ぼすか?

    山口将希, 山口将希, 青山朋樹, 伊藤明良, 伊藤明良, 長井桃子, 太治野純一, 飯島弘貴, ZHANG Xhangkai, 喜屋武弥, 黒木裕士

    超音波骨折治療研究会プログラム・抄録集  2015 

     More details

    Event date: 2015

  38. 変形性膝関節症患者における外側スラストの出現は安静時痛,動作時痛,日常生活動作の困難さに影響する

    福谷直人, 飯島弘貴, 青山朋樹, 山本裕子, 平岡正和, 宮信和幸, 陳之内将志, 金田瑛司, 坪山直生, 松田秀一

    日本理学療法学術大会(Web)  2015  (公社)日本理学療法士協会

     More details

    Event date: 2015

    Language:Japanese  

  39. 骨軟骨欠損モデルラットに対する間葉系間質細胞移植と低出力超音波パルス治療の併用による骨軟骨再生効果の検討

    山口将希, 山口将希, 伊藤明良, 伊藤明良, 太治野純一, 長井桃子, 飯島弘貴, 張項凱, 喜屋武弥, 青山朋樹, 黒木裕士

    日本理学療法学術大会(Web)  2015  (公社)日本理学療法士協会

     More details

    Event date: 2015

    Language:Japanese  

  40. 間欠的な温熱刺激を用いたヒト軟骨細胞による関節軟骨基質生成の試み-三次元培養を用いたin vitro研究-

    伊藤明良, 伊藤明良, 太治野純一, 長井桃子, 山口将希, 飯島弘貴, 張項凱, 喜屋武弥, 青山朋樹, 黒木裕士

    日本理学療法学術大会(Web)  2015  (公社)日本理学療法士協会

     More details

    Event date: 2015

    Language:Japanese  

  41. 運動負荷は損傷した軟骨下骨を治癒させ変形性膝関節症の進行予防に寄与する 二次性変形性膝関節症モデルラットを用いたin vivo研究

    飯島弘貴, 青山朋樹, 伊藤明良, 山口将希, 長井桃子, 太治野純一, 張項凱, 喜屋武弥, 黒木裕士

    日本理学療法学術大会(Web)  2015  (公社)日本理学療法士協会

     More details

    Event date: 2015

    Language:Japanese  

  42. 微小重力環境に対する遠心人工重力介入がラットの歩行パターン変化に与える影響

    太治野純一, 伊藤明, 伊藤明, 長井桃子, 張項凱, 山口将希, 山口将希, 飯島弘貴, 飯島弘貴, 喜屋武弥, 青山朋樹, 黒木裕士

    日本宇宙航空環境医学会大会プログラム・予稿集  2015  日本宇宙航空環境医学会

     More details

    Event date: 2015

    Language:Japanese  

  43. 外側スラストに対する治療介入の可能性-Japan Thrust Contest-

    福谷直人, 飯島弘貴, 青山朋樹

    日本理学療法学術大会(Web)  2015  (公社)日本理学療法士協会

     More details

    Event date: 2015

    Language:Japanese  

  44. 8週間の関節不動により変性した軟骨は,不動解除後に変性が助長される

    長井桃子, 青山朋樹, 伊藤明良, 山口将希, 飯島弘貴, 太治野純一, 張項凱, 秋山治彦, 黒木裕士

    日本理学療法学術大会(Web)  2014  (公社)日本理学療法士協会

     More details

    Event date: 2014

    Language:Japanese  

  45. ラットOAモデルに対する運動の軟骨変性予防効果

    飯島弘貴, 青山朋樹, 伊藤明良, 太治野純一, 長井桃子, ZHANG Xiangkai, 山口将希, 秋山治彦, 黒木裕士

    日本軟骨代謝学会プログラム・抄録集  2014 

     More details

    Event date: 2014

  46. 免荷による歩容変化は再荷重によっても回復しない 後肢免荷がラットの歩容に与える影響

    太治野純一, 伊藤明良, 張項凱, 長井桃子, 山口将希, 飯島弘貴, 青山朋樹, 黒木裕士

    日本理学療法学術大会(Web)  2014  (公社)日本理学療法士協会

     More details

    Event date: 2014

    Language:Japanese  

  47. Development of a ubiquitous treatment method for osteoarthritis

    黒木裕士, 青山朋樹, 長井桃子, 山口将希, 飯島弘貴, 伊藤明良

    大阪ガスグループ福祉財団調査・研究報告集  2014 

     More details

    Event date: 2014

  48. 骨髄由来間葉系間質細胞移植治療後の運動介入が欠損した関節軟骨再生に及ぼす影響

    山口将希, 青山朋樹, 伊藤明良, 長井桃子, 飯島弘貴, 太治野純一, 張項凱, 黒木裕士

    再生医療  2014 

     More details

    Event date: 2014

  49. 骨髄由来間葉系間質細胞移植治療と運動との併用は骨軟骨欠損した関節軟骨の再生を促進する

    山口将希, 青山朋樹, 伊藤明良, 長井桃子, 太治野純一, 飯島弘貴, 張項凱, 秋山治彦, 黒木裕士

    日本理学療法学術大会(Web)  2014  (公社)日本理学療法士協会

     More details

    Event date: 2014

    Language:Japanese  

  50. 関節軟骨基質再生のための至適温度の探究-三次元培養を用いたin vitro研究-

    伊藤明良, 伊藤明良, 青山朋樹, 長井桃子, 太治野純一, 山口将希, 飯島弘貴, 張項凱, 秋山治彦, 黒木裕士

    日本理学療法学術大会(Web)  2014  (公社)日本理学療法士協会

     More details

    Event date: 2014

    Language:Japanese  

  51. 膝関節拘縮後の軟骨基質変化-超微細構造からの検討-

    長井桃子, 伊藤明良, 太治野純一, 飯島弘貴, 山口将希, ZHANG Xiangkai, 青山朋樹, 秋山治彦, 黒木裕士

    日本軟骨代謝学会プログラム・抄録集  2014 

     More details

    Event date: 2014

  52. 精密微細射出成形培養皿による間葉系幹細胞配向性の導出

    張項凱, 安田崇志, 尾池真, 藤岡瑠音, 伊藤明良, 長井桃子, 太治野純一, 山口将希, 飯島弘貴, 柿沼憲宏, 青山朋樹, 黒木裕士

    再生医療  2014 

     More details

    Event date: 2014

  53. 温度環境は軟骨細胞外基質形成能に影響を与える-ヒト軟骨細胞を用いたin vitro研究-

    伊藤明良, 伊藤明良, 青山朋樹, 長井桃子, 太治野純一, 山口将希, 飯島弘貴, ZHANG Xiangkai, 秋山治彦, 黒木裕士

    日本軟骨代謝学会プログラム・抄録集  2014 

     More details

    Event date: 2014

  54. 変形性関節症モデルラットに対する緩徐な走行運動は軟骨下骨変化を予防できるのか?

    飯島弘貴, 青山朋樹, 伊藤明良, 長井桃子, 山口将希, 太治野純一, 張項凱, 秋山治彦, 黒木裕士

    日本理学療法学術大会(Web)  2014  (公社)日本理学療法士協会

     More details

    Event date: 2014

    Language:Japanese  

  55. 下肢および頭部の血流動態に注目した空気圧免荷トレッドミル歩行の安全性の検証

    太治野純一, 伊藤明良, 鳥居勇輔, 土本浩司, 飯島弘貴, 張項凱, 長井桃子, 山口将希, 家城弘, 南角学, 柿木良介, 井上大輔, 広瀬太希, 黒木裕士

    日本理学療法学術大会(Web)  2013  (公社)日本理学療法士協会

     More details

    Event date: 2013

    Language:Japanese  

  56. 寒冷刺激が変形性膝関節症ラットの関節軟骨に及ぼす影響

    山口将希, 伊藤明良, 長井桃子, 張項凱, 飯島弘貴, 太治野純一, 青山朋樹, 秋山治彦, 広瀬太希, 井上大輔, 黒木裕士

    日本理学療法学術大会(Web)  2013  (公社)日本理学療法士協会

     More details

    Event date: 2013

    Language:Japanese  

  57. 関節軟骨におけるHSP70の発現局在の解明と荷重量の変化が及ぼす影響

    広瀬太希, 飯島弘貴, 伊藤明良, 長井桃子, 山口将希, 太治野純一, 張項凱, 井上大輔, 青山朋樹, 秋山治彦, 黒木裕士

    日本理学療法学術大会(Web)  2013  (公社)日本理学療法士協会

     More details

    Event date: 2013

    Language:Japanese  

  58. 軟骨細胞に対する長期温熱刺激の安全性と軟骨基質生合成へ与える影響の検討

    伊藤明良, 伊藤明良, 青山朋樹, 長井桃子, 山口将希, 飯島弘貴, 太治野純一, 張項凱, 井上大輔, 広瀬太希, 秋山治彦, 黒木裕士

    日本理学療法学術大会(Web)  2013  (公社)日本理学療法士協会

     More details

    Event date: 2013

    Language:Japanese  

  59. 膝関節不動が関節軟骨におけるCD44発現に及ぼす影響

    長井桃子, 伊藤明良, 山口将希, 飯島弘貴, 太治野純一, 張項凱, 井上大輔, 広瀬太希, 青山朋樹, 黒木裕士

    日本理学療法学術大会(Web)  2013  (公社)日本理学療法士協会

     More details

    Event date: 2013

    Language:Japanese  

  60. 膝半月板被覆部の軟骨・骨複合体は構造的に脆弱である

    飯島弘貴, 青山朋樹, 伊藤明良, 太治野純一, 張項凱, 長井桃子, 山口将希, 広瀬太希, 井上大輔, 秋山治彦, 黒木裕士

    日本理学療法学術大会(Web)  2013  (公社)日本理学療法士協会

     More details

    Event date: 2013

    Language:Japanese  

  61. 尾部懸垂モデルラットに対する懸垂中断時間が,骨強度,骨密度に与える影響

    井上大輔, 飯島弘貴, 伊藤明良, 長井桃子, 山口将希, 太治野純一, 張項凱, 広瀬太希, 青山朋樹, 秋山治彦, 黒木裕士

    日本理学療法学術大会(Web)  2013  (公社)日本理学療法士協会

  62. 超高齢社会を健康長寿社会へ リハビリテーションを軸とした新学際領域への挑戦 Invited

    飯島 弘貴

    第1回ヘルスサイエンス研究会  2022.4.27 

     More details

    Language:Japanese   Presentation type:Oral presentation (keynote)  

  63. NOTUM -- Transcriptomic Evidence of an Exercise-Responsive Myokine Toward the Preservation of Bone Health: An Exploratory Study

    Iijima H, Chowdhary K, Ambrosio F

    Physiatry’21  2021.2 

     More details

    Language:English   Presentation type:Poster presentation  

  64. Moderate to Severe LBP was Associated with Recurrent Falls in People with Knee Osteoarthritis

    Iijima H, Shimoura K, Aoyama T, Takahashi M

    ORS 2020 Annual Meeting  2020.2 

     More details

    Language:English   Presentation type:Poster presentation  

  65. Mild thermal environment enhances redifferentiation and cartilage extracellular matrix formation of expanded elderly human chondrocytes

    Ito A, Yamaguchi A, Tajino J, Nagai M, Iijima H, Zhang X, Aoyama T, Kuroki H

    Third Annual Symposium on Regenerative Rehabilitation  2014.4 

     More details

    Language:English   Presentation type:Poster presentation  

  66. Markerless Mobility Assessment Techniques Toward an Establishment of Outcome Measures of Regenerative Rehabilitation for Knee Osteoarthritis

    Iijima H, Eguchi R, Yorozu A, Aoyama T, Takahashi M

    7th Annual International Symposium on Regenerative Rehabilitation  2018.11 

     More details

    Language:English   Presentation type:Poster presentation  

  67. Is cartilage and subchondral bone covered by menisci weak?

    Iijima H, Aoyama T, Ito A, Tajino J, Nagai M, Zhang X, Yamaguchi S, Akiyama H, Kuroki H

    2013 World Congress on Osteoarthritis  2013.4 

     More details

    Language:English   Presentation type:Poster presentation  

  68. Intermittent gravitation by centrifuge counteracts against the gait alteration in rats induced by two-week simulated microgravity

    Tajino J, Ito A, Nagai M, Zhang X, Yamaguchi S, Iijima H, Aoyama T, Kuroki H

    20th IAA Humans in Space  2015.6 

     More details

    Language:English   Presentation type:Oral presentation (general)  

  69. Intermittent centrifugation attenuates the disruption of rat walking induced by 2-week hind limb unloading

    Tajino J, Ito A, Nagai M, Zhang X, Yamaguchi S, Iijima H, Aoyama T, Kuroki H

    35th Annual International Gravitational Physiology Meeting  2014.6 

     More details

    Language:English   Presentation type:Oral presentation (general)  

  70. Integrated approach of meta-analysis and bioinformatics towards elucidating disease mechanisms associated with age-related knee osteoarthritis

    Iijima H, Gilmer G, Wang K, Sivakumar S, Matsui Y, Ambrosio F

    OARSI Connect’21  2021.4 

     More details

    Language:English   Presentation type:Oral presentation (general)  

  71. Influence of knee pain on various activities of daily living may reduce physical activity in patients with knee osteoarthritis according to disease severity

    Fukutani N, Iijima H, Fukumoto T, Uritani D, Kaneda E, Ota K, Aoyama T, Tsuboyama T, Matsuda S

    9th World Congress of the International Society of Physical and Rehabilitation Medicine  2015.6 

     More details

    Language:English   Presentation type:Poster presentation  

  72. Identifying the optimal dose of the intermittent gravitation to attenuate the alteration of rat’s walking induced by 2-week simulated microgravity

    Tajino J, Ito A, Nagai M, Zhang X, Yamaguchi S, Iijima H, Aoyama T, Kuroki H

    36th Annual International Gravitational Physiology Meeting  2015.6 

     More details

    Language:English   Presentation type:Poster presentation  

  73. Gait kinematics changes in post-traumatic knee osteoarthritis with destabilized medial meniscus in rat

    Nakahata A, Iijima H, Tanima-Nagai M, Ito A, Tajino J, Kiyan W, Zhang J, Ji X, Wang T, Aoyama T, Nishitani K, Kuroki H

    2018 World Congress on Osteoarthritis  2018.4 

     More details

    Language:English   Presentation type:Poster presentation  

  74. Gait Analysis Of Patients With Distal Radius Fracture Using A Novel Laser-tug System

    Fujita K, Iijima H, Okumura A, Yao Y, Nimura A, Kato R, Okawa A, Takahashi M

    ACSM’s annual meeting  2019.5 

     More details

    Language:English   Presentation type:Poster presentation  

  75. Functional manifestation in patients with early knee osteoarthritis toward the preservation of joint health: A systematic review and meta-analysis

    Kanako S, Shimizu H, Iijima H, Suzuki Y, Aoyama T

    OARSI Connect’21  2021.4 

     More details

    Language:English   Presentation type:Poster presentation  

  76. Efficacy of LIPUS treatment following mesenchymal stromal cell intra-articular injection in an osteochondral defect model rats

    Yamaguchi S, Aoyama T, Ito A, Nagai M, Iijima H, Tajino J, Zhang X, Kiyan W, Kuroki H

    Fourth Annual Symposium on Regenerative Rehabilitation  2015.9 

     More details

    Language:English   Presentation type:Poster presentation  

  77. Efficacy of exercise following bone marrow mesenchymal stromal cell transplantation in an osteochondral defect model of rats

    Yamaguchi S, Aoyama T, Ito A, Nagai M, Iijima H, Tajino J, Zhang X, Akiyama H, Kuroki H

    Third Annual Symposium on Regenerative Rehabilitation  2014.4 

     More details

    Language:English   Presentation type:Poster presentation  

  78. Effects of Transcutaneous Electrical Nerve Stimulation on Stair Climbing Capacity: a Secondary Analysis of Randomized Controlled Trial

    Iijima H, Eguchi R, Shimoura K, Aoyama T, Takahashi M

    ORS 2019 Annual Meeting  2019.2 

     More details

    Language:English   Presentation type:Poster presentation  

  79. Effects of the combined therapy of rehabilitation with cell therapy on motor functional recovery after cerebral infarction: A systematic review

    Kubo N, Ito A, Shimogawa T, Iiijima H, Wang T, Aoyama T, Kuroki H

    7th Annual International Symposium on Regenerative Rehabilitation  2018.11 

     More details

    Language:English   Presentation type:Poster presentation  

  80. Effectiveness of Mesenchymal Stem Cells for Treating Patients with Knee Osteoarthritis: A Meta-analysis Toward the Establishment of Effective Regenerative Rehabilitation

    Iijima H, Isho T, Kuroki H, Takahashi M, Aoyama T

    7th Annual International Symposium on Regenerative Rehabilitation  2018.11 

     More details

    Language:English   Presentation type:Poster presentation  

  81. Effect of unweight-bearing for mechanical properties and ultrastructure of collagen in articular cartilage

    Iijima H, Aoyama T, Ito A, Tajino J, Nagai M, Zhang X, Yamaguchi S, Kuroki H

    The 6th Asia-Western Pacific Regional Congress of the World Confederation for Physical Therapy (WCPT) & The 12th International Congress of Asian Confederation for Physical Therapy (ACPT) Congress 2013  2013.9 

     More details

    Language:English   Presentation type:Poster presentation  

  82. Effect of initiation timing of gentle treadmill exercise on cartilage and subchondral bone in a model of destabilization of medial meniscus of rats

    Iijima H, Aoyama T, Ito A, Yamaguchi S, Nagai M, Tajino J, Zhang X, Kiyan W, Kuroki H

    2015 World Congress on Osteoarthritis  2015.4 

     More details

    Language:English   Presentation type:Poster presentation  

  83. Dynamic regulation of bone morphogenetic proteins by gentle treadmill walking potentially prevent progression of osteoarthritis in a rat model of destabilized medial meniscus

    Iijima H, Aoyama T, Ito A, Tajino J, Yamaguchi S, Nagai M, Zhang X, Kiyan W, Kuroki H

    Fourth Annual Symposium on Regenerative Rehabilitation  2015.9 

     More details

    Language:English   Presentation type:Poster presentation  

  84. Culturing temperature affects chondrocyte differentiation and extracellular matrix formation and redifferentiation of expanded human chondrocyte

    Ito A, Aoyama T, Tajino J, Nagai M, Yamaguchi S, Iijima H, Zhang X, Akiyama H, Kuroki H

    2014 World Congress on Osteoarthritis  2014.4 

     More details

    Language:English   Presentation type:Poster presentation  

  85. Culturing temperature affects chondrocyte differentiation and extracellular matrix formation

    Ito A, Aoyama T, Tajino J, Nagai M, Yamaguchi S, Iijima H, Zhang X, Akiyama H, Kuroki H

    2013 World Congress on Osteoarthritis  2013.4 

     More details

    Language:English   Presentation type:Poster presentation  

  86. Contribution of biarticular myogenic components to limitation of range of motion after joint immobility

    Nagai M, Ito A, Yamaguchi S, Iijima H, Zhang X, Tajino J, Aoyama T, Kuroki H

    Asia-Western Pacific Regional Congress of the World Confederation for Physical Therapy (WCPT) & International Congress of Asian Confederation for Physical Therapy (ACPT) Congress 2013  2013.9 

     More details

    Language:English   Presentation type:Poster presentation  

  87. Concurrent Validity and Measurement Error of Stair Climb Test in Individuals with Knee Osteoarthritis

    Iijima H, Shimoura K, Eguchi R, Aoyama T, Takahashi M

    23th Annual Meeting of the Japanese Association of Physical Therapy Fundamentals  2018.12 

     More details

    Language:English   Presentation type:Oral presentation (general)  

  88. Changes on the expression of CD44 in immobilized knee in rats

    Nagai M, Ito A, Zhang X, Yamaguchi S, Iijima H, Tajino J, Aoyama T, Kuroki H

    2013 World Congress on Osteoarthritis  2013.4 

     More details

    Language:English   Presentation type:Poster presentation  

  89. Cartilage degeneration in immobilization rat knee joint deteriorated with re-mobilization

    Nagai M, Aoyama T, Ito A, Tajino J, Yamaguchi A, Iijima H, Zhang X, Akiyama H, Kuroki H

    Third Annual Symposium on Regenerative Rehabilitation  2014.4 

     More details

    Language:English   Presentation type:Poster presentation  

  90. Biomechanical Characteristics of Stair Ambulation in Patients with Knee OA: A Systematic Review with Meta-analysis Toward A Better Definition of Clinical Hallmarks

    Iijima H, Shimoura K, Aoyama T, Takahashi M

    2018 World Congress on Osteoarthritis  2018.4 

     More details

    Language:English   Presentation type:Poster presentation  

  91. Assessment on the effects of high and low in vivo cyclic compressive loading on the progression of cartilage degeneration in rat knee joint

    Ji X, Nakahata A, Ito A, Iijima H, Tajino J, Tanima-Nagai M, Kiyan W, Zhang J, Wang T, Nishitani K, Aoyama T, Kuroki H

    2018 World Congress on Osteoarthritis  2018.4 

     More details

    Language:English   Presentation type:Poster presentation  

  92. Age-related increase in matrix stiffness downregulates α-Klotho in cartilage and induces cartilage degeneration.

    Iijima H, Gilmer G, Wang K, Bean A, Ambrosio F

    OARSI Connect’21  2021.4 

     More details

    Language:English   Presentation type:Oral presentation (general)  

  93. Age attenuates the benefits of platelet-rich plasma on chondrocyte health

    Chowdhary K, Sahu A, Iijima H, Miller A, Bean A, Ambrosio F

    Physiatry’21  2021.2 

     More details

    Language:English   Presentation type:Poster presentation  

  94. Acceleration patterns of the trunk during walking in individuals with varus thrust: toward an estabilishment of the pathomechanics of varus thrust

    Iijima H, Eguchi R, Aoyama T, Takahashi M

    2018 World Congress on Osteoarthritis  2018.4 

     More details

    Language:English   Presentation type:Poster presentation  

  95. Novel Locomotive Assessment for Knee Osteoarthritis: Laser-TUG

    Iijima H, Yorozu A, Suzuki Y, Eguchi R, Aoyama T, Takahashi M

    ORS 2019 Annual Meeting  2019.2 

     More details

    Language:English   Presentation type:Poster presentation  

  96. Waist-Mounted Sensor Algorithm for Gait Events Detection: A Scoping Review

    Iijima H, Takahashi M

    ORS 2020 Annual Meeting  2020.2 

     More details

    Language:English   Presentation type:Poster presentation  

  97. Trunk movement asymmetry was associated with pain, disability, and quadriceps strength asymmetry in individuals with knee osteoarthritis: a cross-sectional study

    Iijima H, Eguchi R, Aoyama T, Takahashi M

    ORS 2019 Annual Meeting  2019.2 

     More details

    Language:English   Presentation type:Poster presentation  

  98. Treadmill exercise post bone marrow mesenchymal stromal cells transplantation stimulated the regeneration of articular cartilage on rat knee joint osteochondral defect

    Yamaguchi S, Aoyama T, Ito A, Nagai M, Iijima H, Tajino J, Zhang X, Akiyama H, Kuroki H

    2014 World Congress on Osteoarthritis  2014.4 

     More details

    Language:English   Presentation type:Oral presentation (general)  

  99. The Role of Mechanical Force in Tendon Development: A Scoping Review

    Usami Y, Iijima H, Kokubun T

    OARSI Connect’21  2021.4 

     More details

    Language:English   Presentation type:Poster presentation  

  100. The limited knee range of motion causes progression of cartilage degeneration in the osteoarthritic knee joint: an experimental study using a preclinical model of osteoarthritis

    Iijima H, Tanima-Nagai M, Uchiyama K, Ito A, Tajino J, Nakahata A, Kiyan W, Zhang J, Ji X, Wang T, Aoyama T, Nishitani K, Takahashi M, Kuroki H

    2018 World Congress on Osteoarthritis  2018.4 

     More details

    Language:English   Presentation type:Poster presentation  

  101. The influence of re-mobilization on altered cartilage induced by joint immobilization - pathological process of the cyst formation -

    Nagai M, Ito A, Tajino J, Zhang X, Yamaguchi S, Iijima H, Kiyan W, Aoyama T, Kuroki H

    2015 World Congress on Osteoarthritis  2015.4 

     More details

    Language:English   Presentation type:Poster presentation  

  102. The effect of low intensity pulsed ultrasound treatment combined with mesenchymal stromal cell injection for cartilage regeneration in a knee osteochondral defect model of rats

    Yamaguchi S, Aoyama T, Ito A, Nagai M, Iijima H, Tajino J, Zhang X, Kiyan W, Kuroki H

    2015 World Congress on Osteoarthritis  2015.4 

     More details

    Language:English   Presentation type:Poster presentation  

  103. The association of declining klotho expression with an onset of knee osteoarthritis in pre-clinical model and human samples

    Iijima H, He Y, Wang N, Lin H, Ambrosio F

    2020 World Congress on Osteoarthritis  2020.4 

     More details

    Language:English   Presentation type:Oral presentation (general)  

  104. Specific contribution of hip abductor muscle strength to turning movement in individuals with knee osteoarthritis

    Iijima H, Yorozu A, Suzuki Y, Eguchi R, Aoyama T, Takahashi M

    2018 World Congress on Osteoarthritis  2018.4 

     More details

    Language:English   Presentation type:Poster presentation  

  105. Slower belt, but still enough adaptation ~the extent of a “broken escalator phenomenon”~

    Tajino J, Ito A, Nagai M, Zhang X, Yamaguchi S, Iijima H, Kuroki H

    American Physical Therapy Association (APTA) Combined Sections Meeting (CSM)  2014.2 

     More details

    Language:English   Presentation type:Poster presentation  

  106. Slowed turning speed in knee osteoarthritis patients with weaker hip abductor muscle

    Iijima H, Yorozu A, Suzuki Y, Eguchi R, Aoyama T, Takahashi M

    23th Annual Meeting of the Japanese Association of Physical Therapy Fundamentals  2018.12 

     More details

    Language:English   Presentation type:Oral presentation (general)  

  107. Site specific influence of weight or non-weight bearing condition on the stiffness of the cartilage during short-term immobilization intervention on rat knee joint

    Nagai M, Iijima H, Tajino J, Yamaguchi S, Zhang X, Kiyan W, Aoyama T, Kuroki H

    2016 World Congress on Osteoarthritis  2015.3 

     More details

    Language:English   Presentation type:Poster presentation  

  108. Sarcopenic Knee Osteoarthritis: A Risk Factor for Recurrent Falls

    Iijima H, Aoyama T

    ORS 2020 Annual Meeting  2020.2 

     More details

    Language:English   Presentation type:Poster presentation  

  109. Role of the Anti-Aging Factor Alpha-Klotho in Aging-related Knee Osteoarthritis: A Pilot Study

    Iijima H, Wang N, Lin H, Ambrosio F

    8th Annual International Symposium on Regenerative Rehabilitation  2019.11 

     More details

    Language:English   Presentation type:Poster presentation  

  110. Risk-benefit balance of repetitive physical exercise on osteoarthritic articular cartilage: a cross-cutting systematic review of human, in vivo, and ex vivo studies

    Iijima H, Sonoo M, Yamashita T

    23th Annual Meeting of the Japanese Association of Physical Therapy Fundamentals  2018.12 

     More details

    Language:English   Presentation type:Oral presentation (general)  

  111. Reloading does not attenuate the hindlimb unload-induced gait alteration: The influence of tail suspension to gait pattern of rats

    Tajino J, Ito A, Zhang X, Nagai M, Yamaguchi S, Iijima H, Aoyama T, Kuroki H

    2014 World Congress on Osteoarthritis  2014.4 

     More details

    Language:English   Presentation type:Poster presentation  

  112. Relationship Between Varus Thrust During Gait and Low Back Pain in People with Knee Osteoarthritis

    Iijima H, Suzuki Y, Aoyama T, Matsuda S, Takahashi M

    ORS 2019 Annual Meeting  2019.2 

     More details

    Language:English   Presentation type:Poster presentation  

  113. Rehabilitative Exercise-driven Cartilage Regeneration: A Cross-Cutting Systematic Review from Ex Vivo, Animal, and Clinical Trials

    Iijima H, Sonoo M

    8th Annual International Symposium on Regenerative Rehabilitation  2019.11 

     More details

    Language:English   Presentation type:Poster presentation  

  114. Rehabilitation program after mesenchymal stem cell therapy in patients with knee osteoarthritis –a systematic review of clinical study-

    Iijima H, Isho T, Kuroki H, Aoyama T

    The 30th Annual Meeting of the Japanese Society of Cartilage Metabolism  2017.3 

     More details

    Language:English   Presentation type:Oral presentation (general)  

  115. Rehabilitation After Osteochondral Autograft Transplantation: A Single Case Report

    Iijima H, Takeda T, Harada S, Iwadate D, Otsuka K

    7th Annual International Symposium on Regenerative Rehabilitation  2018.11 

     More details

    Language:English   Presentation type:Poster presentation  

  116. Regional changes of early osteoarthritis cartilage and subchondral bone fracture in a rat knee model of OA

    Iijima H, Aoyama T, Ito A, Yamaguchi S, Nagai M, Tajino J, Zhang X, Akiyama H, Kuroki H

    2014 World Congress on Osteoarthritis  2014.4 

     More details

    Language:English   Presentation type:Poster presentation  

  117. Re-mobilization of knees aggravated cartilage degeneration in an immobilization model of rats

    Nagai M, Aoyama T, Ito A, Yamaguchi S, Iijima H, Tajino J, Zhang X, Akiyama H, Kuroki H

    2014 World Congress on Osteoarthritis  2014.4 

     More details

    Language:English   Presentation type:Poster presentation  

  118. Quadriceps strength in individuals with coexisting medial and lateral osteoarthritis: toward an identification of modifiable risk factors in important subgroup of mild radiographic disease

    Iijima H, Suzuki Y, Aoyama T, Takahashi M

    2018 World Congress on Osteoarthritis  2018.4 

     More details

    Language:English   Presentation type:Poster presentation  

  119. Proximal Gait Adaptations in Individuals with Medial Knee OA: A Systematic Review with Meta-analysis

    Iijima H, Takahashi M

    2018 World Congress on Osteoarthritis  2018.4 

     More details

    Language:English   Presentation type:Poster presentation  

  120. Poor Stair Climbing Capacity in Early Knee Osteoarthritis: Toward a Defining Clinical Hallmarks of Early Disease

    Iijima H, Eguchi R, Shimoura K, Aoyama T, Takahashi M

    ORS 2019 Annual Meeting  2019.2 

     More details

    Language:English   Presentation type:Poster presentation  

  121. Physiological exercise loading suppresses post-traumatic osteoarthritis progression via increase in bone morphogenetic proteins expression in an experimental rat knee model

    Iijima H, Ito A, Nagai M, Tajino J, Yamaguchi S, Kiyan W, Nakahata A, Zhang J, Wang T, Aoyama T, Nishitani K, Kuroki H

    2017 World Congress on Osteoarthritis  2017.4 

     More details

    Language:English   Presentation type:Poster presentation  

  122. Periodic heat stimulus for extracellular matrix production on human chondrocytes

    Ito A, Iijima H, Tajino J, Nagai M, Yamaguchi S, Zhang X, Aoyama T, Kuroki H

    Fourth Annual Symposium on Regenerative Rehabilitation  2015.9 

     More details

    Language:English   Presentation type:Poster presentation  

  123. People With Varus Thrust Less Responds to Home-Based Quadriceps Exercise on Pain Outcomes: A Secondary Subgroup Analysis From a Randomized Controlled Trial

    Iijima H, Suzuki Y, Aoyama T, Matsuda S, Takahashi M

    ORS 2019 Annual Meeting  2019.2 

     More details

    Language:English   Presentation type:Poster presentation  

  124. Patellofemoral osteoarthritis progression related with gait kinematics changes in rat with destabilized medial meniscus

    Nakahata A, Iijima H, Tanima-Nagai M, Ito A, Wang T, Nakahara R, Tajino J, Zhang J, Kawai H, Kubo N, Aoyama T, Kuroki H

    7th Annual International Symposium on Regenerative Rehabilitation  2018.11 

     More details

    Language:English   Presentation type:Poster presentation  

▼display all

Research Project for Joint Research, Competitive Funding, etc. 7

  1. 加齢性疾患をドライブするエピジェネティック制御機構の探求

    2022.4 - 2027.3

    世界的課題を解決する知の『開拓者』育成事業 

      More details

    Authorship:Principal investigator 

  2. Optimizing Muscle and Bone Mechanoadaptation to Physical Training: Understanding the Mechanistic Pathways via Muscle Bone Crosstalk to Altered Mechanical Loading

    2021.9 - 2026.8

    The U.S. Army Medical Research and Development Command Grant 

      More details

    Authorship:Coinvestigator(s) 

  3. From platelet dust to extracellular vesicles – the mechanistic study of platelet-rich plasma and its role in the treatment of osteoarthritis

    2020.11 - 2021.10

    Scott F. Nadler PASSOR Musculoskeletal Research Grant 

      More details

    Authorship:Coinvestigator(s) 

  4. 骨髄間葉系幹細胞移植に最適な関節内微小環境を目指すニッチェリハビリテーション

    2019.5 - 2021.3

    海外特別研究員 

      More details

    Authorship:Principal investigator 

  5. マルチセンサの有機的統合による運動器不安定症発症予測アルゴリズム開発

    2018.4 - 2020.3

    科学研究費助成事業 若手研究 

      More details

    Authorship:Principal investigator 

  6. 変形性膝関節症の動的診断システム開発によるテーラーメードリハビリテーションの創出

    2017.4 - 2020.3

    科学研究費助成事業 特別研究員奨励費 

      More details

    Authorship:Principal investigator 

  7. 変形性膝関節症モデルラットに対する運動負荷がもたらす病理組織学的変化

    2015.4 - 2017.3

    科学研究費助成事業 特別研究員奨励費 

      More details

    Authorship:Principal investigator 

▼display all

Industrial property rights 1

  1. Methods and materials for treating osteoarthritis

    Hirotaka Iijima, Fabrisia Ambrosio

     More details

    Application no:US Patent App. 17/236,726  Date applied:2021.4