2024/03/11 更新

写真a

ミヤケ ナツキ
三宅 菜月
MIYAKE Natsuki
所属
医学部附属病院 産科婦人科 助教
大学院担当
大学院医学系研究科
職名
助教

学位 2

  1. 博士(医学) ( 2022年10月   名古屋大学 ) 

  2. 学士(医学) ( 2011年3月   名古屋大学 ) 

 

論文 12

  1. Serum miRNA as a predictive biomarker for ovarian reserve after endometrioma-cystectomy 査読有り

    Yabuki, A; Muraoka, A; Osuka, S; Yokoi, A; Yoshida, K; Kitagawa, M; Bayasura; Sonehara, R; Miyake, N; Nakanishi, N; Nakamura, T; Iwase, A; Kajiyama, H

    REPRODUCTIVE BIOLOGY   24 巻 ( 1 ) 頁: 100821   2024年3月

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    記述言語:英語   出版者・発行元:Reproductive Biology  

    Ovarian endometrioma (OE) is a common gynecological disease that is often treated with surgery and hormonal treatment. However, ovarian cystectomy can impair the ovarian reserve (OR). Previously, we showed that perioperative administration of dienogest (DNG) is an effective option for OR preservation. However, there were differences in the extent of OR preservation among patients following perioperative DNG treatment. In the current study, we performed a global examination of serum microRNAs (miRNAs) to identify accurate biomarkers that predict post-operative restoration of OR following perioperative DNG treatment. We also sought to identify specific miRNAs related to the anti-Müllerian hormone (AMH). miRNA sequencing was performed on serum samples obtained from twenty-seven patients who received perioperative DNG treatment. Candidate miRNAs were selected by comparing patients whose ORs were restored postoperatively (responder group, n = 7) with those whose ORs were not (non-responder group, n = 7). miR-370–3p and miR-1307–3p were significantly upregulated in the responder group, whereas miR-27b-3p was upregulated in the non-responder group. The pretreatment value of each miRNA could predict DNG responsiveness for OR following ovarian cystectomy (area under the curve [AUC] > 0.8). The quantitative polymerase chain reaction (qPCR) revealed only miR-1307–3p was found to be significantly upregulated in the responder group (P < 0.05). In addition, we identified miR-139–3p, miR-140–3p, and miR-629–5p as AMH-associated miRNAs. The transition of AMH showed a correlation with miR-139–3p (P < 0.05, r = −0.76). The miRNAs identified herein represent potential serum biomarkers of clinical value in predicting OR prior to DNG treatment.

    DOI: 10.1016/j.repbio.2023.100821

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  2. Small extracellular vesicles in follicular fluids for predicting reproductive outcomes in assisted reproductive technology.

    Muraoka A, Yokoi A, Yoshida K, Kitagawa M, Asano-Inami E, Murakami M, Bayasula, Miyake N, Nakanishi N, Nakamura T, Osuka S, Iwase A, Kajiyama H

    Communications medicine   4 巻 ( 1 ) 頁: 33   2024年2月

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    記述言語:英語  

    DOI: 10.1038/s43856-024-00460-8

    PubMed

  3. The influence of radical trachelectomy on endometrial thickness in in vitro fertilization-embryo transfer 査読有り

    Yabuki, A; Muraoka, A; Tamauchi, S; Seki, T; Takeda, T; Sonehara, R; Miyake, N; Nakamura, T; Osuka, S; Kajiyama, H

    JOURNAL OF OBSTETRICS AND GYNAECOLOGY RESEARCH   50 巻 ( 2 ) 頁: 218 - 224   2024年2月

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    記述言語:英語   出版者・発行元:Journal of Obstetrics and Gynaecology Research  

    Aim: Both morbidity and mortality rates of cervical cancer are increasing, especially in reproductive-aged women. Radical trachelectomy (RT) is an effective fertility-preserving surgery for early-stage cervical cancer. This study aimed to determine the influence of RT on endometrial thickness during in vitro fertilization-embryo transfer (IVF-ET). Methods: Forty-four patients had undergone RT, and 23 women undergoing IVF-ET treatment (105 ET cycles) were included. Endometrial thickness during hormone replacement therapy (HRT) was retrospectively evaluated and compared between patients with and without RT. Results: Eleven patients (50 ET cycles) in the RT group and 12 (52 ET cycles) in the control group were investigated. Compared with the control group, higher ET cancellation rates were observed in patients in the RT group (1 of 52 cycles [control group] vs. 8 of 50 cycles [RT group], p < 0.01). Endometrial thinning was not affected by patient age at first IVF-ET treatment, history of artificial abortion, preservation of uterine arteries during RT, or postoperative chemotherapy (p = 0.27, 1, 1, and 1, respectively). Conclusions: Our data revealed that RT influenced endometrial thickness in IVF-ET. This was not affected by the background of the patients or perioperative management in this study. We could not reveal the underlying mechanism, but it is postulated that the transient postoperative uterine blood flow status and postoperative infections may have some effect on the endometrium. To resolve these issues, accumulation of evidences are required. We recommend informing patients about the impact of RT on IVF-ET before starting assisted reproductive technology (ART).

    DOI: 10.1111/jog.15841

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  4. Unkeito promotes follicle development by restoring reduced follicle-stimulating hormone responsiveness in rats with polycystic ovary syndrome 査読有り

    Yoshita, S; Osuka, S; Shimizu, T; Fujitsuka, N; Matsumoto, C; Bayasula; Miyake, N; Muraoka, A; Nakanishi, N; Nakamura, T; Goto, M; Kajiyama, H

    FRONTIERS IN ENDOCRINOLOGY   14 巻   頁: 1228088   2023年9月

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    記述言語:英語   出版者・発行元:Frontiers in Endocrinology  

    Background: Polycystic ovary syndrome (PCOS) is a common disorder resulting in irregular menstruation and infertility due to improper follicular development and ovulation. PCOS pathogenesis is mediated by downregulated follicle-stimulating hormone receptor (FSHR) expression in granulosa cells (GCs); however, the underlying mechanism remains elusive. Unkeito (UKT) is a traditional Japanese medicine used to treat irregular menstruation in patients with PCOS. In this study, we aimed to confirm the effectiveness of UKT in PCOS by focusing on follicle-stimulating hormone (FSH) responsiveness. Methods: A rat model of PCOS was generated by prenatal treatment with 5α-dihydrotestosterone. Female offspring (3-week-old) rats were fed a UKT mixed diet or a normal diet daily. To compare the PCOS phenotype in rats, the estrous cycle, hormone profiles, and ovarian morphology were evaluated. To further examine the role of FSH, molecular, genetic, and immunohistological analyses were performed using ovarian tissues and primary cultured GCs from normal and PCOS model rats. Results: UKT increased the number of antral and preovulatory follicles and restored the irregular estrous cycle in PCOS rats. The gene expression levels of FSHR and bone morphogenetic protein (BMP)-2 and BMP-6 were significantly decreased in the ovarian GCs of PCOS rats compared to those in normal rats. UKT treatment increased FSHR staining in the small antral follicles and upregulated Fshr and Bmps expression in the ovary and GCs of PCOS rats. There was no change in serum gonadotropin levels. In primary cultured GCs stimulated by FSH, UKT enhanced estradiol production, accompanied by increased intracellular cyclic adenosine monophosphate levels, and upregulated the expression of genes encoding the enzymes involved in local estradiol synthesis, namely Cyp19a1 and Hsd17b. Furthermore, UKT elevated the expression of Star and Cyp11a1, involved in progesterone production in cultured GCs in the presence of FSH. Conclusions: UKT stimulates ovarian follicle development by potentiating FSH responsiveness by upregulating BMP-2 and BMP-6 expression, resulting in the recovery of estrous cycle abnormalities in PCOS rats. Restoring the FSHR dysfunction in the small antral follicles may alleviate the PCOS phenotype.

    DOI: 10.3389/fendo.2023.1228088

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  5. Unkeito (Wen-jing-tang) Decreases Ovulation Disorder in a Rat Model of Polycystic Ovary Syndrome 査読有り

    Osuka Satoko, Yoshita Sayako, Shimizu Tomofumi, Matsumoto Chinami, Seki Tomomi, Miyake Natsuki, Sonehara Reina, Muraoka Ayako, Nananishi Natsuki, Nakamura Tomoko, Kajiyama Hiroaki

    REPRODUCTIVE SCIENCES   30 巻   頁: 250A - 251A   2023年1月

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  6. Association Between Anti-Mullerian Hormone Levels and Polycystic Ovarian Syndrome in a General Cohort of Young Women in Japan

    Miyake Natsuki, Osuka Satoko, Goto Maki, Seki Tomomi, Muraoka Ayako, Nakanishi Natsuki, Nakamura Tomoko, Iwase Akira, Kajiyama Hiroaki

    REPRODUCTIVE SCIENCES   30 巻   頁: 172A - 172A   2023年1月

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  7. 東海北陸地方における小児・思春期血液がん患者のがん・生殖相談外来受診状況に対する多機関共同・後ろ向き観察研究 査読有り

    竹中 基記, 前沢 忠志, 齋藤 愛, 坊本 佳優, 三宅 菜月, 榎本 紗也子, 西岡 美喜子, 松本 洋介, 伊東 雅美, 宮崎 有美子, 松本 雅子, 大須賀 智子, 後藤 真紀, 安藤 智子, 古井 辰郎

    日本小児血液・がん学会雑誌   60 巻 ( 1 ) 頁: 20 - 26   2023年

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    記述言語:日本語   出版者・発行元:日本小児血液・がん学会  

    <p>2020年8月に東海北陸若手がん・生殖ネットワークが発足し,がん・生殖医療に関する情報共有や啓発活動を行っている.今回,小児・思春期血液がん患者の生殖機能に関する医療連携の現状を調査し,課題の抽出や今後の展望を見出した.</p><p>2013年1月1日~2021年6月30日までに,当ネットワーク参加9施設のがん・生殖医療外来を受診した小児・思春期の血液がん患者(年齢18歳まで)について,後方視的に検討した.</p><p>全症例115例(男性71例,女性44例)で,年齢別では9歳以下が4例,10–12歳が9例,13–15歳が42例,16–18歳が60例であった.精子凍結は希望した66例中13例が完遂できなかったが,その原因は無精子症が大半を占めていた.卵巣組織凍結は希望した17例中16例が完遂でき,がん治療中で造血細胞移植前処置前のタイミングで行われている症例が多かった.また,7例に周術期の輸血を要した.未受精卵子凍結は希望した11例中8例が完遂でき,思春期前(14歳以下)の女児でも完遂例が4例みられた.</p><p>小児・思春期の血液がん患者におけるがん・生殖医療は,若年になるにつれ情報提供・紹介は少なく,妊孕性温存療法を行うにあたっても骨髄抑制などリスクを伴い,その実施には注意を要する.しかし,妊孕性温存療法のニーズやその完遂率は比較的高いため,今後も特に思春期前の小児を中心に患者・医療関係者含め啓発を続けていきたい.</p>

    DOI: 10.11412/jspho.60.20

    CiNii Research

  8. Differentiation of human induced pluripotent stem cells into hypothalamic vasopressin neurons with minimal exogenous signals and partial conversion to the naive state 査読有り

    Ozaki Hajime, Suga Hidetaka, Sakakibara Mayu, Soen Mika, Miyake Natsuki, Miwata Tsutomu, Taga Shiori, Nagai Takashi, Kano Mayuko, Mitsumoto Kazuki, Miyata Takashi, Kobayashi Tomoko, Sugiyama Mariko, Onoue Takeshi, Takagi Hiroshi, Hagiwara Daisuke, Iwama Shintaro, Banno Ryoichi, Iguchi Genzo, Takahashi Yutaka, Muguruma Keiko, Inoue Haruhisa, Arima Hiroshi

    SCIENTIFIC REPORTS   12 巻 ( 1 ) 頁: 17381   2022年10月

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    記述言語:英語   出版者・発行元:Scientific Reports  

    Familial neurohypophyseal diabetes insipidus (FNDI) is a degenerative disease of vasopressin (AVP) neurons. Studies in mouse in vivo models indicate that accumulation of mutant AVP prehormone is associated with FNDI pathology. However, studying human FNDI pathology in vivo is technically challenging. Therefore, an in vitro human model needs to be developed. When exogenous signals are minimized in the early phase of differentiation in vitro, mouse embryonic stem cells (ESCs)/induced pluripotent stem cells (iPSCs) differentiate into AVP neurons, whereas human ESCs/iPSCs die. Human ESCs/iPSCs are generally more similar to mouse epiblast stem cells (mEpiSCs) compared to mouse ESCs. In this study, we converted human FNDI-specific iPSCs by the naive conversion kit. Although the conversion was partial, we found improved cell survival under minimal exogenous signals and differentiation into rostral hypothalamic organoids. Overall, this method provides a simple and straightforward differentiation direction, which may improve the efficiency of hypothalamic differentiation.

    DOI: 10.1038/s41598-022-22405-8

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  9. Upregulated Ribosomal Pathway Impairs Follicle Development in a Polycystic Ovary Syndrome Mouse Model: Differential Gene Expression Analysis of Oocytes 査読有り

    Nakanishi Natsuki, Osuka Satoko, Kono Tomohiro, Kobayashi Hisato, Ikeda Shinya, Bayasula Bayasula, Sonehara Reina, Murakami Mayuko, Yoshita Sayako, Miyake Natsuki, Muraoka Ayako, Kasahara Yukiyo, Murase Tomohiko, Nakamura Tomoko, Goto Maki, Iwase Akira, Kajiyama Hiroaki

    REPRODUCTIVE SCIENCES     2022年10月

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    記述言語:英語   出版者・発行元:Reproductive Sciences  

    Polycystic ovary syndrome (PCOS), a common endocrine disorder, is associated with impaired oocyte development, leading to infertility. However, the pathogenesis of PCOS has not been completely elucidated. This study aimed to determine the differentially expressed genes (DEGs) and epigenetic changes in the oocytes from a PCOS mouse model to identify the etiological factors. RNA-sequencing analysis revealed that 90 DEGs were upregulated and 27 DEGs were downregulated in mice with PCOS compared with control mice. DNA methylation analysis revealed 30 hypomethylated and 10 hypermethylated regions in the PCOS group. However, the DNA methylation status did not correlate with differential gene expression. The pathway enrichment analysis revealed that five DEGs (Rps21, Rpl36, Rpl36a, Rpl37a, and Rpl22l1) were enriched in ribosome-related pathways in the oocytes of mice with PCOS, and the immunohistochemical analysis revealed significantly upregulated expression levels of Rps21 and Rpl36. These results suggest that differential gene expression in the oocytes of mice in PCOS is related to impaired folliculogenesis. These findings improve our understanding of PCOS pathogenesis.

    DOI: 10.1007/s43032-022-01095-7

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  10. Predictive factors for massive hemorrhage in women with retained products of conception: a prospective study 査読有り

    Sonehara Reina, Nakamura Tomoko, Iwase Akira, Nishida Kazuki, Takikawa Sachiko, Murakami Mayuko, Yoshita Sayako, Muraoka Ayako, Miyake Natsuki, Nakanishi Natsuki, Osuka Satoko, Goto Maki, Kajiyama Hiroaki

    SCIENTIFIC REPORTS   12 巻 ( 1 ) 頁: 11859   2022年7月

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    記述言語:英語   出版者・発行元:Scientific Reports  

    Retained products of conception (RPOC) is a common cause of postpartum bleeding, which may be life-threatening; however, no evidence-based guidelines exist to assist in evaluating the risk of massive hemorrhage in women with RPOC. In this prospective study, we aimed to evaluate the predictive factors for massive hemorrhage in women with RPOC. The primary and secondary endpoints were to validate the usefulness of power Doppler color scoring (PDCS) in evaluating hypervascularity and to identify other predictive factors (such as maximum RPOC diameter and serum βhCG and Hb level at first visit), respectively. Among the 51 women with RPOC included in this study, 16 (31.5%) experienced massive hemorrhage during follow-up. None of the women with PDCS 1 or 2 (18) experienced massive hemorrhage, whereas 16 (48.5%) women with PDCS 3 or 4 (33) did. Multiple logistic regression analysis showed that the odds ratio [95% confidence interval] (P value) for PDCS, assisted reproductive technology (ART), and low serum hemoglobin (Hb) levels were 22.39 [2.25 − 3087.92] (P = 0.004), 5.72 [1.28 − 33.29] (P = 0.022), and 4.24 [0.97 − 22.99] (P = 0.056), respectively. Further, the decision tree method identified PDCS, ART, and low serum Hb levels as potential predictive factors for massive hemorrhage. This study identified PDCS as useful predictor of massive hemorrhage in women with RPOC. With additional inclusion of factors such as ART and low serum Hb levels, the risk of massive hemorrhage may be effectively evaluated, leading to better management of women of reproductive age.

    DOI: 10.1038/s41598-022-15564-1

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  11. Effectiveness of NLRP3 Inhibitor as a Non-Hormonal Treatment for ovarian endometriosis 査読有り

    Murakami Mayuko, Osuka Satoko, Muraoka Ayako, Hayashi Shotaro, Bayasula, Kasahara Yukiyo, Sonehara Reina, Hariyama Yumi, Shinjo Kanako, Tanaka Hideaki, Miyake Natsuki, Yoshita Sayako, Nakanishi Natsuki, Nakamura Tomoko, Goto Maki, Kajiyama Hiroaki

    REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY   20 巻 ( 1 ) 頁: 58   2022年3月

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    記述言語:英語   出版者・発行元:Reproductive Biology and Endocrinology  

    Background: Endometriosis is a complex syndrome characterized by an estrogen-dependent chronic inflammatory process that affects 10% of women of reproductive age. Ovarian endometriosis (OE) is the most common lesion in endometriosis and may cause infertility, in addition to dysmenorrhea. Hormonal treatments, which are the conventional treatment methods for endometriosis, suppress ovulation and hence are not compatible with fertility. The inflammasome is a complex that includes Nod-like receptor (NLR) family proteins, which sense pathogen-associated molecular patterns and homeostasis-altering molecular processes. It has been reported that the nucleotide-binding oligomerization domain, leucine-rich repeat, and pyrin domain-containing (NLRP) 3 inflammasome, which contributes to the activation of interleukin-1 beta (IL-1β), might be related to the progression of endometriosis. Therefore, the aim of the present study was to evaluate non-hormonal therapies for OE, such as inhibitors of the NLRP3 inflammasome. Methods: The expression of NLRP3 was measured in the eutopic endometrium (EM) of patients with and without endometriosis and OE samples, as well as stromal cells derived from the endometrium of patients with and without endometriosis and OE samples (endometrial stromal cells with endometriosis [ESCs] and cyst-derived stromal cells [CSCs]). The effects of an NLRP3 inhibitor (MCC950) on ESCs and CSCs survival and IL-1β production were evaluated. We then administered MCC950 to a murine model of OE to evaluate its effects on OE lesions and ovarian function. Results: NLRP3 gene and protein expression levels were higher in OE and CSCs than in EM and ESCs, respectively. MCC950 treatment significantly reduced the survival of CSCs, but not that of ESCs. Moreover, MCC950 treatment reduced the co-localization of NLRP3 and IL-1β in CSCs, as well as IL-1β concentrations in CSCs supernatants. In the murine model, MCC950 treatment reduced OE lesion size compared to phosphate-buffered saline treatment (89 ± 15 vs. 49 ± 9.3 mm3 per ovary; P < 0.05). In the MCC950-treated group, IL-1β and Ki67 levels in the OE-associated epithelia were reduced along with the oxidative stress markers of granulosa cells. Conclusions: These results indicated that NLRP3/IL-1β is involved in the pathogenesis of endometriosis and that NLRP3 inhibitors may be useful for suppressing OE and improving the function of ovaries with endometriosis.

    DOI: 10.1186/s12958-022-00924-3

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  12. Functional Lactotrophs in Induced Adenohypophysis Differentiated From Human iPS Cells 査読有り

    Miyake Natsuki, Nagai Takashi, Suga Hidetaka, Osuka Satoko, Kasai Takatoshi, Sakakibara Mayu, Soen Mika, Ozaki Hajime, Miwata Tsutomu, Asano Tomoyoshi, Kano Mayuko, Muraoka Ayako, Nakanishi Natsuki, Nakamura Tomoko, Goto Maki, Yasuda Yoshinori, Kawaguchi Yohei, Miyata Takashi, Kobayashi Tomoko, Sugiyama Mariko, Onoue Takeshi, Hagiwara Daisuke, Iwama Shintaro, Iwase Akira, Inoshita Naoko, Arima Hiroshi, Kajiyama Hiroaki

    ENDOCRINOLOGY   163 巻 ( 3 )   2022年3月

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    担当区分:筆頭著者   記述言語:日本語   出版者・発行元:Endocrinology (United States)  

    Prolactin (PRL), a hormone involved in lactation, is mainly produced and secreted by the lactotrophs of the anterior pituitary (AP) gland. We previously reported a method to generate functional adrenocorticotropic hormone-producing cells by differentiating the AP and hypothalamus simultaneously from human induced pluripotent stem cells (iPSCs). However, PRL-producing cells in the induced AP have not been investigated. Here, we confirmed the presence of PRL-producing cells and evaluated their endocrine functions. We differentiated pituitary cells from human iPSCs using serum-free floating culture of embryoid-like aggregates with quick reaggregation (SFEB-q) method and evaluated the appearance and function of PRL-producing cells. Secretion of PRL from the differentiated aggregates was confirmed, which increased with further culture. Fluorescence immunostaining and immunoelectron microscopy revealed PRL-producing cells and PRL-positive secretory granules, respectively. PRL secretion was promoted by various prolactin secretagogues such as thyrotropin-releasing hormone, vasoactive intestinal peptide, and prolactin-releasing peptide, and inhibited by bromocriptine. Moreover, the presence of tyrosine hydroxylase-positive dopaminergic nerves in the hypothalamic tissue area around the center of the aggregates connecting to PRL-producing cells indicated the possibility of recapitulating PRL regulatory mechanisms through the hypothalamus. In conclusion, we generated pituitary lactotrophs from human iPSCs; these displayed similar secretory responsiveness as human pituitary cells in vivo. In the future, this is expected to be used as a model of human PRL-producing cells for various studies, such as drug discovery, prediction of side effects, and elucidation of tumorigenic mechanisms using disease-specific iPSCs. Furthermore, it may help to develop regenerative medicine for the pituitary gland.

    DOI: 10.1210/endocr/bqac004

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▼全件表示

講演・口頭発表等 3

  1. マウスES細胞からの視床下部キスペプチンニューロンの分化誘導法の検討

    三宅菜月、須賀英隆、大須賀智子、関友望、井上直子、上野山賀久、小谷友美、梶山広明、束村博子、有馬寛

    第96回日本内分泌学会学術総会  2023年6月  日本内分泌学会

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    開催年月日: 2023年6月

    記述言語:日本語   会議種別:ポスター発表  

    開催地:名古屋国際会議場   国名:日本国  

  2. In vitro differentiation of Kiss1 neuron in the hypothalamus from mouse embryonic stem cells

    Natsuki Miyake, Satoko Osuka, Tomomi Seki, Takehiko Takeda, Satoshi Kaseki, Hideaki Tanaka, Atsushi Yabuki, Reina Sonehara, Ayako Muraoka, Natsuki Nakanishi, Tomoko Nakamura, Hiroaki Kajiyama

    2023年5月12日 

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    開催年月日: 2023年5月

    記述言語:英語   会議種別:口頭発表(一般)  

    国名:日本国  

  3. Association Between Anti-Müllerian Hormone Levels and Polycystic Ovarian Syndrome in a General Cohort of Young Women in Japan 国際会議

    Natsuki Miyake, Satoko Osuka, Maki Goto, Akira Iwase, Ayako Muraoka, Natsuki Nakanishi, Tomoko Nakamura, Hiroaki Kajiyama

    The 70th Annual Meeting of the Society for Reproductive Investigation  2023年3月23日  the Society for Reproductive Investigation

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    開催年月日: 2023年3月

    記述言語:英語   会議種別:ポスター発表  

    開催地:the Brisbane Convention & Exhibition Centre   国名:オーストラリア連邦  

科研費 2

  1. 早発排卵機序の解明を目指したヒトiPS細胞由来生殖中枢のin vitroモデル作成

    研究課題/研究課題番号:23K15807  2023年4月 - 2026年3月

    科学研究費助成事業  若手研究

    三宅 菜月

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    担当区分:研究代表者 

    配分額:4290000円 ( 直接経費:3300000円 、 間接経費:990000円 )

    既報を基に、ヒト多能性幹細胞から下垂体オルガノイドを分化させる過程で様々な誘導因子を添加し、LH/FSH産生細胞の効率的な分化を目指す。適宜、前駆細胞のマーカー遺伝子の発現を確認しながら至適培養条件を検討する。LH/FSH細胞が分化したら、GnRHを添加し、生体内と同様の反応を示すことを確認するとともに、ヒト非黄体化顆粒膜細胞との共培養や下垂体除去動物への移植でE2分泌の亢進や性機能の回復を確認する。最終的には既存および新規のin vitroモデルと組み合わせることで視床下部―下垂体―性腺軸の完全モデルを作成する。

  2. 早発卵巣不全予測マーカー測定法開発と妊孕性温存療法への展開

    研究課題/研究課題番号:23K08865  2023年4月 - 2026年3月

    科学研究費助成事業  基盤研究(C)

    大須賀 智子, 村岡 彩子, 三宅 菜月

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    担当区分:研究分担者 

    昨今の不妊治療技術の向上により、これまで妊娠を諦めざるを得なかった不妊症の患者が子を持つことが可能となってきた。一方で、早発卵巣不全や加齢による妊孕能の低下等、現在の不妊治療でも克服できない問題もあり難治性不妊症と呼ばれる。早発卵巣不全は40歳未満で卵子が枯渇し閉経状態に至る疾患である。不可逆性であり一旦成立すると自身の卵子で妊娠することはほぼ不可能である。事前に予測できれば卵子凍結や卵巣組織凍結による妊孕性温存が技術的に可能であるが、現在そのようなマーカーはない。
    本研究は、新規妊孕能マーカーの測定によりこのような不可逆性不妊が成立する前にリスク例を検出することを目的とする。