Updated on 2025/03/15

写真a

 
TAKAHASHI Yasufumi
 
Organization
Graduate School of Engineering Electronics 2 Professor
Graduate School
Graduate School of Engineering
Undergraduate School
School of Engineering Electrical Engineering, Electronics, and Information Engineering
Title
Professor

Degree 1

  1. 博士(学術) ( 2009.3 ) 

Research Areas 1

  1. Nanotechnology/Materials / Analytical chemistry  / 走査型プローブ顕微鏡

Research History 8

  1. Nagoya University   Graduate School of Engineering Electronics   Professor

    2021.12

  2. Kanazawa University   Professor

    2020.10

  3. Kanazawa University   Associate Professor

    2017.10 - 2020.9

  4. Kanazawa University   Associate Professor

    2015.10 - 2017.9

  5. Tohoku University   Assistant Professor

    2013.4 - 2015.9

▼display all

Education 2

  1. Tohoku University

    2006.4 - 2009.3

  2. Tohoku University

    2004.4 - 2006.3

Professional Memberships 5

  1. THE ELECTROCHEMICAL SOCIETY OF JAPAN

  2. THE BIOPHYSICAL SOCIETY OF JAPAN

  3. THE CHEMICAL SOCIETY OF JAPAN

  4. THE JAPAN SOCIETY FOR ANALYTICAL CHEMISTRY

  5. THE JAPAN SOCIETY OF APPLIED PHYSICS

Awards 20

  1. 2022堀場雅夫賞

    2022.10   堀場財団   触媒活性サイトの実空間イメージングに資する 電気化学セル顕微鏡の開発

    高橋康史

  2. 第16回わかしゃち奨励賞

    2022.1   愛知県経済産業局産業部産業科学技術課   ウィルスの取り込み過程の可視化に資する新奇プローブ顕微鏡の開発

    高橋康史

  3. 中谷賞奨励賞

    2020.1   公益財団法人中谷医工計測技術振興財団  

  4. 第1回バイオインダストリー奨励賞

    2017.10   超解像度ケミカルイメージングを実現する電気化学顕微鏡の開発

    高橋 康史

  5. 若手優秀発表賞

    2017.6   日本細胞生物学会   非標識・非侵襲でのナノスケールの形状観察を実現する走査型イオンコンダクタンス顕微鏡の開発と応用

    高橋 康史

▼display all

 

Papers 148

  1. Direct Extraction and Evaluation of Intraluminal Vesicles Inside a Single Cell. International journal

    Hiroki Ida, Takeshi Yoshida, Akichika Kumatani, Rikinari Hanayama, Yasufumi Takahashi

    Nano letters     2025.2

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    Because endogenous extracellular vesicles are involved in important physiological functions, various techniques have been developed for their isolation and evaluation. However, methods for evaluating endogenous vesicles within cells are limited. This study presents a technique for the direct extraction and evaluation of intraluminal vesicles (ILVs). This technique combines scanning ion conductance microscopy, electrochemical syringes, and confocal microscopy to extract specific structures within a living cell, achieving high spatial resolution and accuracy at the femtoliter scale. This approach allowed the direct collection of CD63(+) vesicles from HEK293 CD63-pHluorin-RFP cells and showed that their RNA expression profiles were different from those recovered from cytosol and extracellular vesicles isolated by ultracentrifuge. It also identified a subset specifically containing hsa-miR-145-5p and allowed for direct assessment of the local accumulation of miRNAs in cells. This technique is expected to become a powerful tool for evaluating the contents of ILVs within living cells.

    DOI: 10.1021/acs.nanolett.4c06315

    PubMed

  2. Lattice-guided growth of dense arrays of aligned transition metal dichalcogenide nanoribbons with high catalytic reactivity

    Zongpeng Ma, Pablo Solís-Fernández, Kaito Hirata, Yung-Chang Lin, Keisuke Shinokita, Mina Maruyama, Kota Honda, Tatsuki Kato, Aika Uchida, Hiroto Ogura, Tomohiro Otsuka, Masahiro Hara, Kazunari Matsuda, Kazu Suenaga, Susumu Okada, Toshiaki Kato, Yasufumi Takahashi, Hiroki Ago

    Science Advances   Vol. 11 ( 2 )   2025.1

     More details

    Publishing type:Research paper (scientific journal)   Publisher:American Association for the Advancement of Science (AAAS)  

    Transition metal dichalcogenides (TMDs) exhibit unique properties and potential applications when reduced to one-dimensional (1D) nanoribbons (NRs), owing to quantum confinement and high edge densities. However, effective growth methods for self-aligned TMD NRs are still lacking. We demonstrate a versatile approach for lattice-guided growth of dense, aligned MoS <sub>2</sub> NR arrays via chemical vapor deposition (CVD) on anisotropic sapphire substrates, without tailored surface steps. This method enables the synthesis of NRs with widths below 10 nanometers and longitudinal axis parallel to the zigzag direction, being also extensible to the growth of WS <sub>2</sub> NRs and MoS <sub>2</sub> -WS <sub>2</sub> heteronanoribbons. Growth is influenced by both substrate and CVD temperature, indicating the role of anisotropic precursor diffusion and substrate interaction. The 1D nature of the NRs was asserted by the observation of Coulomb blockade at low temperatures. Pronounced catalytic activity was observed at the edges of the NRs, indicating their promise for efficient catalysis.

    DOI: 10.1126/sciadv.adr8046

  3. EMT-Induced Morphological Variations on Living Cell Membrane Surface

    Hiroki Ida, Noriko Taira, Yuji Nashimoto, Akichika Kumatani, Yasufumi Takahashi, Hitoshi Shiku

    Analytical Chemistry     2025.1

     More details

    Publishing type:Research paper (scientific journal)   Publisher:American Chemical Society (ACS)  

    DOI: 10.1021/acs.analchem.4c04204

  4. Crystalline Formation Enhances Hydrogen Evolution Reaction Property of Copper Azaphthalocyanine on Carbon Electrodes

    Kosuke Ishibashi, Tengyi Liu, Yuya Ishizaki, Shusaku Nagano, Junya Yoshida, Shimpei Ono, Yasufumi Takahashi, Akichika Kumatani, Hiroshi Yabu

    ACS Applied Energy Materials     2024.11

     More details

    Publishing type:Research paper (scientific journal)   Publisher:American Chemical Society (ACS)  

    DOI: 10.1021/acsaem.4c02102

  5. Probing non-Faradaic process during elastic deformation in a single sphere of extremely soft mesoporous carbon

    Kritin Pirabul, Zheng-Ze Pan, Kazuya Kanamaru, Yoshiko Horiguchi, Yasufumi Takahashi, Akichika Kumatani, Hirotomo Nishihara

    Carbon   Vol. 228   page: 119376 - 119376   2024.9

     More details

    Publishing type:Research paper (scientific journal)   Publisher:Elsevier BV  

    DOI: 10.1016/j.carbon.2024.119376

▼display all

Books 5

  1. Local Electrochemical Characterization Using Scanning Electrochemical Cell Microscopy

    Makarova M.V., Takahashi Y.

    Bioanalytical Reviews  2022 

     More details

    Scanning electrochemical cell microscopy (SECCM) is a unique tool for nanoscale electrochemical imaging for characterizing sample reactivity. It uses a mobile meniscus state electrochemical cell between the nanopipette and the sample. The chapter describes the method setup and principles as well as its reported applications in materials sciences. SECCM was successfully used to image various types of reactions such as hydrogen/oxygen evolution, oxygen/CO2 reduction, corrosion, photocatalysis, and charge/discharge processes in battery materials. The studied objects included carbon phases: diamond and graphite with their derivatives; polymers; transitional metal dichalcogenides; metals; Li complex oxides. SECCM demonstrated site-dependent electrochemical activity, particularly, the role of defects and grain boundaries. Combination of SECCM with EBSD provided the information about the correlation between electrochemical reactivity and terminating facet in multiple kinds of 3D materials. Moreover, SECCM can be used not only to image but also to build new patterned on-surface (sub)microstructures by loading the precursors into the pipette or by site-dependent electrooxidation/reduction of the surface. Thus, the advantages and goals of SECCM for various applications are described in the chapter.

    DOI: 10.1007/11663_2021_12

    Scopus

  2. 生きてるものは全部観る! イメージングの選び方・使い方100+

    高橋 康史( Role: Contributor ,  45.原子間力顕微鏡 ⅱ.走査型イオンコンダクタンス顕微鏡―生細胞の形状と機能をナノスケールで可視化)

    羊土社  2018.12 

  3. Ion conductance probe microscopy-molecular resolution

    Zhou Y., Fukuma T., Takahashi Y.

    Encyclopedia of Interfacial Chemistry: Surface Science and Electrochemistry  2018.1  ( ISBN:9780128097397

     More details

    Language:Japanese

    Scanning ion-conductance microscopy (SICM) is a nanopipette-based technique that has been widely used in noncontact topography imaging, particularly for imaging cell surfaces. Recent studies have considerably improved the spatiotemporal resolution of SICM based on miniaturization of the probe and development of new scanning mode. In addition, SICM can provide nanoscale functional information of sample surface alone or combined with other techniques. In this article, we focus on these functional imaging and sensing applications of SICM. First, we highlight the combination of SICM with electrochemical sensing. For example, when SICM is combined with scanning electrochemical microscopy, the surface topography and chemical activity can be imaged simultaneously. Second, we introduce SICM for surface-charge mapping and other nanopore-based platforms. The ion current rectification effect that is dependent on the interaction of transported ions with the surface charge of both nanopipette itself and substrate have been used for these applications. When SICM is combined with chemical modified nanopipettes, ion channels, proteins, as well as intracellular pH and glucose can be detected with high spatial resolution. Finally, we discuss SICM probes used for the nanoscale extraction of intracellular molecules from single living cells. The extracted samples can be used to access cell functions by molecular biology techniques.

    DOI: 10.1016/B978-0-12-409547-2.13510-3

    Scopus

  4. Nanobiosensors and Nanobioanalyses

    Mun'delanji C. Vestergaard, Kagan Kerman, I-Ming Hsing, Eiichi Tamiya

    Springer  2015.3  ( ISBN:4431551891

     More details

    Total pages:379  

    ASIN

  5. バイオ電気化学の実際―バイオセンサ・バイオ電池の実用展開 (CMCテクニカルライブラリー 456 バイオテクノロジーシリーズ)

    池田篤治

    シーエムシー出版  2013.4  ( ISBN:4781307159

     More details

    Total pages:314  

    ASIN

MISC 36

  1. Electrochemical Imaging of Catalytically Active Site by Scanning Electrochemical Cell Microscopy

    平田海斗, 高橋康史, 高橋康史

    表面と真空   Vol. 67 ( 5 )   2024

     More details

  2. Alkaline Phosphatase-based Electrochemical Analysis for Point-of-Care Testing

    Yusuke Kanno, Yuanshu Zhou, Takeshi Fukuma, Yasufumi Takahashi

    Electroanalysis   Vol. 34 ( 2 ) page: 161 - 167   2022.2

     More details

    Publishing type:Book review, literature introduction, etc.  

    Point-of-care testing (POCT) devices have evolved to provide beneficial information about an individual's health whenever needed. Enzyme-based analytical devices have facilitated the highly selective detection of numerous biological molecules and ions. Enzymes are commonly used as the tags of recognition components, such as antibodies, to generate and amplify detection signals. Particularly, alkaline phosphatase (ALP) is one of the most widely used enzymes because of its high turnover number and low cost. Rapid response time and the incorporation of many sensors fabricated by micro/nano processing technologies are the advantages in using electrochemical devices as analytical tools. Therefore, ALP-based electrochemical devices have potential applications for more practical POCT platforms. This review summarizes recent research progress of ALP-based electrochemical devices for POCT. In addition to ALP substrates, the application of ALP-based immunosensors, aptasensors, and DNAzyme sensors are discussed.

    DOI: 10.1002/elan.202100294

    Web of Science

    Scopus

  3. 高速走査型イオンコンダクタンス顕微鏡による細胞表面の微小構造動態の非侵襲測定

    井田大貴, 高橋康史, 珠玖仁, 末永智一

    第1回細胞生物若手の会     2015.6

     More details

    Language:Japanese   Publishing type:Research paper, summary (national, other academic conference)  

  4. CMOS型電気化学デバイスを用いた神経細胞塊から放出される神経伝達物質のイメージング

    阿部博弥, 伊野浩介, LI Chen‐Zhong, 菅野祐介, 井上久美, 國方亮太, 須田篤史, 松平昌昭, 高橋康史, 珠玖仁, 末永智一

    電気化学会大会講演要旨集(CD-ROM)   Vol. 82nd   page: ROMBUNNO.PS31   2015.3

     More details

    Language:Japanese  

    J-GLOBAL

  5. ナノ電気化学セル顕微鏡:有機ゲルピペットの電池材料評価への応用

    熊谷明哉, 高橋康史, 猪又宏貴, 白木将, 山本邦子, 春田正和, 伊野浩介, 珠玖仁, 一杉太郎, 末永智一

    電気化学会大会講演要旨集(CD-ROM)   Vol. 82nd   page: ROMBUNNO.PBT03   2015.3

     More details

    Language:Japanese  

    J-GLOBAL

▼display all

Presentations 179

  1. 走査型プローブ顕微鏡を用いた生細胞の動的イメージング Invited

    髙橋康史

    JST CREST 統合1細胞解析のための革新的技術基盤 非標識神経伝達物質イメージセンサによる細胞活動可視化システム構築と脳機能の時空間解析 2020 CREST 合同ミーティング in 中伊豆  2020.1.30 

     More details

    Event date: 2020.1

    Language:Japanese   Presentation type:Public lecture, seminar, tutorial, course, or other speech  

  2. Nanoscale Electrochemical Imaging using Scanning Probe Microscopy Invited

    Yasufumi Takahashi

    1st WPI NanoLSI-iCeMS Joint Symposium on Nanoimaging and Advanced Materials for Life Science  2020.1.23 

     More details

    Event date: 2020.1

    Language:English   Presentation type:Symposium, workshop panel (nominated)  

  3. Visualization of Inhomogeneous Reactivity Using Scanning Electrochemical Cell Microscopy Invited

    Yasufumi Takahashi

    Electrochemistry Gordon Research Conference  2020.1.10 

     More details

    Event date: 2020.1

    Language:English   Presentation type:Oral presentation (invited, special)  

  4. Live cell functional imaging using scanning probe microscopy Invited International conference

    TAKAHASHI Yasufumi

    3rd NanoLSI Symposium at UBC in Vancouver  2019.8.8 

     More details

    Language:English   Presentation type:Symposium, workshop panel (nominated)  

  5. ナノ電気化学セル顕微鏡を用いたグラファイト/グラフェン表面の電気化学活性の可視化

    熊谷明哉, 高橋康史, 三浦千穂, 珠玖仁, 末永智一, 末永智一

    電気化学会大会講演要旨集(CD-ROM)  2016.3.23 

     More details

    Language:Japanese  

▼display all

KAKENHI (Grants-in-Aid for Scientific Research) 19

  1. Biological role of GOMED and related diseases

    Grant number:23H05480  2023.4 - 2028.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (S)

  2. Metal tolerance and metal adsorption through phycosphere control

    Grant number:23H02303  2023.4 - 2027.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

  3. ナノ空間を巧みに利用した革新的ナノポアケミカルセンサーの創成

    Grant number:22K18939  2022.6 - 2025.3

    日本学術振興会  科学研究費助成事業 挑戦的研究(萌芽)  挑戦的研究(萌芽)

    高橋 康史

      More details

    Authorship:Principal investigator 

    Grant amount:\6500000 ( Direct Cost: \5000000 、 Indirect Cost:\1500000 )

    細胞内でオルガネラは相互作用しながら活動しており、その理解には、細胞内の化学動態を計測する技術がもとめられている。そこで、ガラスピペットに酵素やアプタマーを修飾し、化学物質の濃度をセンサー薄膜の電荷状態の変化としてとらえる。さらに、能動的に生体分子をナノポア内に導入する手法、導入した生体分子の増幅法を確立し、細胞の代謝物をセンシング可能な革新的なケミカルセンサーを確立する。

  4. 生体膜ナノ構造の可視化と脂質網羅分析のための統合ナノ分析システム

    Grant number:22K18950  2022.6 - 2024.3

    日本学術振興会  科学研究費助成事業 挑戦的研究(萌芽)  挑戦的研究(萌芽)

    川井 隆之, 高橋 康史

      More details

    Authorship:Coinvestigator(s) 

    生体膜の局所的ナノ構造を可視化し,構成する分子組成を明らかにするための「統合ナノ分析システム」を世界で初めて実現し,生体膜ナノ構造に基づく未知の生命原理や疾患の分子機序を明らかにするための技術基盤を構築する。生細胞膜のナノ構造を可視化できる走査型イオン伝導顕微鏡でナノドメインを捕捉して採取し,ナノキャピラリー電気泳動-質量分析で解析する統合ナノ分析システムを開発する。これにより,ナノドメインの時空間情報と脂質分子組成などの化学情報の両面から生体膜の実態解明を目指す。

  5. 2.5次元材料における局所触媒活性の実空間イメージング

    Grant number:22H05459  2022.6 - 2024.3

    日本学術振興会  科学研究費助成事業 学術変革領域研究(A)  学術変革領域研究(A)

    高橋 康史

      More details

    Authorship:Principal investigator 

    Grant amount:\6760000 ( Direct Cost: \5200000 、 Indirect Cost:\1560000 )

    2次元材料の触媒活性向上に向けて、モアレ、歪、欠陥、ドーピング、ヘテロジャンクションの形成など様々な試みがなされてきた。このような構造を巧みに利用した触媒開発において、触媒能と構造の関係性の理解は喫緊の課題である。本研究では、電気化学イメージング技術である走査型電気化学セル顕微鏡(SECCM)を用いて2.5次元材料の触媒活性サイトを、実空間で電気化学的に可視化する。

▼display all

Industrial property rights 14

  1. 走査型イオンコンダクタンス顕微鏡

    高橋 康史

     More details

    Applicant:国立大学法人金沢大学

    Application no:特願2023-028942  Date applied:2023.2

    Announcement no:特開2024-121700  Date announced:2024.9

    J-GLOBAL

  2. 走査プローブ顕微鏡用の探針とその作製方法

    横田 泰之, 小林 柚子, 高橋 康史

     More details

    Applicant:国立研究開発法人理化学研究所

    Application no:特願2023-010322  Date applied:2023.1

    Announcement no:特開2024-106166  Date announced:2024.8

    J-GLOBAL

  3. リアクタ

    平井 健二, 高橋 康史

     More details

    Applicant:国立大学法人北海道大学

    Application no:特願2021-007115  Date applied:2021.1

    Announcement no:特開2021-178311  Date announced:2021.11

    J-GLOBAL

  4. 走査型イオンコンダクタンス顕微鏡

    高橋 康史

     More details

    Applicant:国立大学法人金沢大学

    Application no:特願2020-137427  Date applied:2020.8

    Announcement no:特開2022-033497  Date announced:2022.3

    Patent/Registration no:特許第7492252号  Date registered:2024.5 

    J-GLOBAL

  5. 走査型イオンコンダクタンス顕微鏡

    高橋 康史

     More details

    Applicant:国立大学法人金沢大学

    Application no:特願2020-137427  Date applied:2020.8

    Announcement no:特開2022-033497  Date announced:2022.3

    J-GLOBAL

▼display all

 

Media Coverage 12

  1. 立方体型の超原子が結合した2次元シートを創出~高効率な水素発生触媒の開発に期待~ Internet

    科学技術振興機構(JST)  日本経済新聞  Adv. Mater. 2024, 36, 2404249.  2024.7

  2. 非対称な二次元シートを利用したナノサイズの巻物構造の実現 〜高性能な触媒や発電デバイスへの応用に期待〜 Internet

    名古屋大学  名古屋大学  ACS Nano 2024 18 (4), 2772-2781  2024.1

  3. 生細胞の表面構造をナノスケールで直接可視化 ~エクソソームなど細胞間コミュニケーションの理解に貢献~ Internet

    名古屋大学  名古屋大学  Analytical Chemistry 2023, 95 (34), 12664-12672.  2023.8

  4. 二酸化炭素の還元触媒について、構造と電気化学特性の関係をナノスケールで解明 ~副反応を抑えた二酸化炭素還元のための触媒開発に貢献~ Internet

    名古屋大学  名古屋大学  Acs Nano 2023, 17 (12), 11318-11326.  2023.6

  5. 充放電中のイオンの濃度プロファイルを形状変化とともにナノスケールで可視化 ~デバイス材料の開発・オペレーション条件の最適化に貢献~ Internet

    名古屋大学  名古屋大学  JACS Au 2023, 3 (4), 1089-1099.  2023.3

▼display all