Updated on 2023/10/16

写真a

 
SUZUMURA Ayana
 
Organization
Nagoya University Hospital Ophthalmology Assistant professor of hospital
Title
Assistant professor of hospital

Degree 1

  1. 博士(医学) ( 2020.4   名古屋大学 ) 

Research Areas 1

  1. Life Science / Ophthalmology

Awards 2

  1. 福田賞

    2021.10   日本糖尿病眼学会  

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    Award type:Award from Japanese society, conference, symposium, etc. 

  2. CIBoGリトリート 医学奨励賞

    2021.2   名古屋大学・大学院医学系研究科  リトリート事務局  

 

Papers 23

  1. Retinal ferroptosis as a critical mechanism for the induction of retinochoroiditis during ocular toxoplasmosis

    Yamada K., Tazaki A., Ushio-Watanabe N., Usui Y., Takeda A., Matsunaga M., Suzumura A., Shimizu H., Zheng H., Ariefta N.R., Yamamoto M., Hara H., Goto H., Sonoda K.H., Nishiguchi K.M., Kato M., Nishikawa Y., Toyokuni S., Kaneko H.

    Redox Biology   Vol. 67   page: 102890   2023.11

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    Language:English   Publisher:Redox Biology  

    Toxoplasmosis is a major infectious disease, affecting approximately one-third of the world's population; its main clinical manifestation, ocular toxoplasmosis (OT), is a severe sight-threatening disease. Nevertheless, the diagnosis of OT is based on clinical findings, which needs improvement, even with biochemical tests, such as polymerase chain reaction and antibody detections. Furthermore, the efficacy of OT-targeted treatment is limited; thus, additional measures for diagnosis and treatments are needed. Here, we for the first time report a significantly reduced iron concentration in the vitreous humor (VH) of human patients infected with OT. To obtain further insights into molecular mechanisms, we established a mouse model of T. gondii infection, in which intravitreally injected tracer 57Fe, was accumulated in the neurosensory retina. T. gondii-infected eyes showed increased lipid peroxidation, reduction of glutathione peroxidase-4 expression and mitochondrial deformity in the photoreceptor as cristae loss. These findings strongly suggest the involvement of ferroptotic process in the photoreceptor of OT. In addition, deferiprone, an FDA-approved iron chelator, reduced the iron uptake but also ameliorated toxoplasma-induced retinochoroiditis by reducing retinal inflammation. In conclusion, the iron levels in the VH could serve as diagnostic markers and iron chelators as potential treatments for OT.

    DOI: 10.1016/j.redox.2023.102890

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  2. Association Between Torque Teno Virus and Systemic Immunodeficiency in Patients With Uveitis With a Suspected Infectious Etiology

    Sajiki Ai fujita, Koyanagi Yoshito, Ushida Hiroaki, Kawano Kenichi, Fujita Kosuke, Okuda Daishi, Kawabe Mitsuki, Yamada Kazuhisa, Suzumura Ayana, Kachi Shu, Kaneko Hiroki, Komatsu Hiroyuki, Usui Yoshihiko, Goto Hiroshi, Nishiguchi Koji M.

    AMERICAN JOURNAL OF OPHTHALMOLOGY   Vol. 254   page: 80 - 86   2023.10

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    Language:English   Publisher:American Journal of Ophthalmology  

    PURPOSE: To determine the correlation between the presence of torque teno virus (TTV) in the aqueous humor of patients with uveitis and clinical information, including immunodeficiency history. DESIGN: Multicenter, retrospective, cross-sectional study. METHODS: Fifty-eight patients with uveitis with a suspected infectious etiology and 24 controls with cataract or age-related macular degeneration were included. We used quantitative polymerase chain reaction to test all subjects for TTV and multiplex polymerase chain reaction to test uveitis subjects for common ocular pathogens. When possible, both serum and aqueous humor samples were tested. Ocular TTV positivity was compared with age, sex, and a history of systemic immunodeficiency with logistic analysis. RESULTS: Ocular TTV positivity was found in 23%, 11%, and 0% of patients with herpetic uveitis, nonherpetic uveitis, and controls, respectively. Among patients with herpes infection, positivity for ocular TTV was found in 43%, 8%, 14%, and 50% of patients with cytomegalovirus retinitis, iridocyclitis, acute retinal necrosis, and Epstein–Barr virus–positive uveitis, respectively. Patients with cytomegalovirus retinitis showed a significantly higher rate of ocular TTV infection than controls (P =.008). Serum analysis revealed TTV positivity in 90% of patients with uveitis and in 100% of controls. Age- and gender-adjusted logistic analysis revealed a correlation between ocular TTV positivity and systemic immunodeficiency (P =.01), but no correlations between ocular TTV and age, gender, or viral pathogenic type. CONCLUSIONS: These findings suggest that positivity for ocular TTV was correlated with a clinical history of systemic immunodeficiency.

    DOI: 10.1016/j.ajo.2023.06.012

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  3. Dimethyl Fumarate Protects Retinal Pigment Epithelium from Blue Light-Induced Oxidative Damage via the Nrf2 Pathway

    Shimizu Hideyuki, Takayama Kei, Yamada Kazuhisa, Suzumura Ayana, Sato Tomohito, Nishio Yoshiaki, Ito Masataka, Ushida Hiroaki, Nishiguchi Koji M., Takeuchi Masaru, Kaneko Hiroki

    ANTIOXIDANTS   Vol. 12 ( 1 )   2023.1

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    Language:English   Publisher:Antioxidants  

    The purpose of this study is to investigate the protective effect of dimethyl fumarate (DMF), the methyl-ester of fumaric acid, against blue-light (BL) exposure in retinal pigment epithelial (RPE) cells. ARPE-19 cells, a human RPE cell line, were cultured with DMF followed by exposure to BL. Reactive oxygen species (ROS) generation, cell viability, and cell death rate were determined. Real-time polymerase chain reaction and Western blotting were performed to determine the change in nuclear factor (erythroid-derived)-like 2 (NRF2) expression. Twenty-seven inflammatory cytokines in the supernatant of culture medium were measured. BL exposure induced ROS generation in ARPE-19 cells, which DMF alleviated in a concentration-dependent manner. BL exposure increased the ARPE-19 cell death rate, which DMF alleviated. BL exposure induced ARPE-19 cell apoptosis, again alleviated by DMF. Under BL exposure, DMF increased the NRF2 mRNA level and promoted NRF2 expression in the nucleus. BL also strongly increased interleukin (IL)-1β and fibroblast growth factor (FGF) expression. BL strongly induced RPE cell damage with apoptotic change while DMF mainly reduced inflammation in BL-induced RPE damage, resulting in blockade of cell death. DMF has a protective effect in RPE cells against BL exposure via activation of the NRF2 pathway.

    DOI: 10.3390/antiox12010045

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  4. Oxidative Stress-Induced Cellular Senescence in Aging Retina and Age-Related Macular Degeneration

    Ryo Terao, Tazbir Ahmed, Ayana Suzumura, Hiroko Terasaki

    Antioxidants   Vol. 11 ( 11 )   2022.11

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.3390/antiox11112189

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  5. Protective Effects and Molecular Signaling of n-3 Fatty Acids on Oxidative Stress and Inflammation in Retinal Diseases. International journal

    Ayana Suzumura, Ryo Terao, Hiroki Kaneko

    Antioxidants (Basel, Switzerland)   Vol. 9 ( 10 ) page: 1 - 25   2020.10

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    Oxidative stress and inflammation play crucial roles in the development and progression of retinal diseases. Retinal damage by various etiologies can result in retinopathy of prematurity (ROP), diabetic retinopathy (DR), and age-related macular degeneration (AMD). n-3 fatty acids are essential fatty acids and are necessary for homeostasis. They are important retinal membrane components and are involved in energy storage. n-3 fatty acids also have antioxidant and anti-inflammatory properties, and their suppressive effects against ROP, DR, and AMD have been previously evaluated. α-linolenic acid (ALA), eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA), and their metabolites have been shown to alleviate retinal oxidative stress and inflammation involving various biological signaling pathways. In this review, we summarize the current understanding of the n-3 fatty acids effects on the mechanisms of these retinal diseases and how they exert their therapeutic effects, focusing on ALA, EPA, DHA, and their metabolites. This knowledge may provide new remedial strategies for n-3 fatty acids in the prevention and treatment of retinal diseases associated with oxidative stress and inflammation.

    DOI: 10.3390/antiox9100920

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  6. Caveolin-1 Promotes Cellular Senescence in Exchange for Blocking Subretinal Fibrosis in Age-Related Macular Degeneration. International journal

    Hideyuki Shimizu, Kazuhisa Yamada, Ayana Suzumura, Keiko Kataoka, Kei Takayama, Masataka Sugimoto, Hiroko Terasaki, Hiroki Kaneko

    Investigative ophthalmology & visual science   Vol. 61 ( 11 ) page: 21 - 21   2020.9

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    Language:Japanese   Publishing type:Research paper (scientific journal)  

    Purpose: To determine whether caveolin-1 (i) prevents epithelial-mesenchymal transition in the RPE and laser-induced subretinal fibrosis and (ii) promotes or inhibits cellular senescence in the RPE. Methods: We examined laser-induced subretinal fibrosis and RPE cell contraction in wild-type and Caveolin-1 knockout (Cav-1-/-) mice treated with or without cavtratin, a cell-permeable peptide of caveolin-1. The senescence marker p16INK4a was measured in RPE tissues from patients with geographic atrophy and aged mice, laser-induced subretinal fibrosis, and primary human RPE cells. Human RPE was examined by TUNEL staining, reactive oxygen species generation, cell viability, and senescence-associated β-galactosidase staining. Results: The volume of subretinal fibrosis was significantly smaller in cavtratin-injected eyes from wild-type mice than in control eyes from wild-type, P = 0.0062, and Cav-1-/- mice, P = 0.0095. Cavtratin treatment produced significant improvements in primary RPE cell contraction in wild-type, P = 0.04, and Cav-1-/- mice, P = 0.01. p16INK4a expression in the RPE was higher in patients with than without geographic atrophy. p16INK4a was expressed in 18-month-old but not 2-month-old wild-type mouse eyes. p16INK4a and collagen type I antibodies showed co-localization in subretinal fibrosis. Cavtratin did not affect RPE cell apoptosis or reactive oxygen species generation, but decreased cell viability and increased senescence-associated β-galactosidase-positive cells. Conclusions: Enhanced expression of caveolin-1 successfully blocked epithelial-mesenchymal transition of RPE and the reduction of subretinal fibrosis in mice. Nevertheless, in exchange for blocking subretinal fibrosis, caveolin-1 promotes RPE cellular senescence and might affect the progression of geographic atrophy in AMD.

    DOI: 10.1167/iovs.61.11.21

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  7. Lamivudine Inhibits Alu RNA-induced Retinal Pigment Epithelium Degeneration via Anti-inflammatory and Anti-senescence Activities. International journal

    Kazuhisa Yamada, Hiroki Kaneko, Hideyuki Shimizu, Ayana Suzumura, Rina Namba, Kei Takayama, Seina Ito, Masataka Sugimoto, Hiroko Terasaki

    Translational vision science & technology   Vol. 9 ( 8 ) page: 1 - 1   2020.7

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    Purpose: Accumulation of the long noncoding Alu element RNA activates the NLRP3 inflammasome and leads to retinal pigment epithelium (RPE) cell death, a key event in the pathogenesis of geographic atrophy during late-stage age-related macular degeneration. Lamivudine (3TC) is a nucleoside analog reverse transcriptase inhibitor known to inhibit the NLRP3 inflammasome. Currently, the intracellular response of the senescence marker p16Ink4a to the long noncoding RNA is being actively studied. The present study aimed to assess the efficacy of 3TC against Alu RNA-induced RPE inflammation and senescence by evaluating changes in expression of the proinflammatory cytokines IL-18 and IL-1β and of p16INK4a in RPE cells. Methods: Cultured human RPE cells and in vivo mouse RPE cells were transfected with an in vitro-transcribed Alu RNA, and changes in IL-18, IL-1β, and p16Ink4a expression measured in the presences of 3TC or 3,4-(M)CA as a negative control. Results: Treatment with 3TC markedly reduced Alu RNA-induced expression of IL-18 and IL-1β in human and mouse RPE cells compared with the negative control. Further, Alu RNA-induced p16INK4a expression was suppressed by 3TC in human RPE cells. Conclusions: Our data suggest that Alu RNA accumulation contributes to RPE cell senescence in age-related macular degeneration and that this pathogenic process can be suppressed by 3TC. Translational Relevance: Further verifying this study leads to potential targets for age-related macular degeneration therapy.

    DOI: 10.1167/tvst.9.8.1

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  8. n-3 Fatty Acid and Its Metabolite 18-HEPE Ameliorate Retinal Neuronal Cell Dysfunction by Enhancing Müller BDNF in Diabetic Retinopathy. International journal

    Ayana Suzumura, Hiroki Kaneko, Yasuhito Funahashi, Kei Takayama, Masatoshi Nagaya, Seina Ito, Toshiaki Okuno, Toshiaki Hirakata, Norie Nonobe, Keiko Kataoka, Hideyuki Shimizu, Rina Namba, Kazuhisa Yamada, Fuxiang Ye, Yoko Ozawa, Takehiko Yokomizo, Hiroko Terasaki

    Diabetes   Vol. 69 ( 4 ) page: 724 - 735   2020.4

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    Diabetic retinopathy (DR) is a widespread vision-threatening disease, and neuroretinal abnormality should be considered as an important problem. Brain-derived neurotrophic factor (BDNF) has recently been considered as a possible treatment to prevent DR-induced neuroretinal damage, but how BDNF is upregulated in DR remains unclear. We found an increase in hydrogen peroxide (H2O2) in the vitreous of patients with DR. We confirmed that human retinal endothelial cells secreted H2O2 by high glucose, and H2O2 reduced cell viability of MIO-M1, Müller glia cell line, PC12D, and the neuronal cell line and lowered BDNF expression in MIO-M1, whereas BDNF administration recovered PC12D cell viability. Streptozocin-induced diabetic rats showed reduced BDNF, which is mainly expressed in the Müller glia cell. Oral intake of eicosapentaenoic acid ethyl ester (EPA-E) ameliorated BDNF reduction and oscillatory potentials (OPs) in electroretinography (ERG) in DR. Mass spectrometry revealed an increase in several EPA metabolites in the eyes of EPA-E-fed rats. In particular, an EPA metabolite, 18-hydroxyeicosapentaenoic acid (18-HEPE), induced BDNF upregulation in Müller glia cells and recovery of OPs in ERG. Our results indicated diabetes-induced oxidative stress attenuates neuroretinal function, but oral EPA-E intake prevents retinal neurodegeneration via BDNF in Müller glia cells by increasing 18-HEPE in the early stages of DR.

    DOI: 10.2337/db19-0550

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  9. Diacron reactive oxygen metabolites and biological antioxidant potential tests for patients with age-related macular degeneration. International journal

    Toshiyuki Matsuura, Hiroki Kaneko, Kei Takayama, Rei Shibata, Keiko Kataoka, Seina Ito, Taichi Tsunekawa, Hideyuki Shimizu, Ayana Suzumura, Rina Namba, Yasuki Ito, Toyoaki Murohara, Hiroko Terasaki

    BMC ophthalmology   Vol. 20 ( 1 ) page: 56 - 56   2020.2

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    BACKGROUND: Previously, we showed that serum malondialdehyde (MDA) was significantly higher in patients with neovascular age-related macular degeneration (nAMD) than in those without AMD. The Diacron reactive oxygen metabolites (d-ROMs) and biological antioxidant potential (BAP) tests are known markers of oxidative stress. The aim of this study was to use d-ROMs and BAP tests to evaluate changes in systemic oxidative stress in patients with nAMD. METHODS: Blood serum samples were collected from 34 patients with nAMD (mean age: 76.5 ± 7.7 years; 22 men) and 20 control subjects (mean age: 62.9 ± 14.0 years; 10 men), and d-ROMs and BAP tests were examined. RESULTS: In men, the mean level of d-ROMs for the nAMD patients was significantly higher than that for the controls (312.0 ± 52.4 vs. 275.1 ± 45.5 U.CARR, respectively; P < .05). There was a significant correlation between d-ROM level and CNV lesion area in the male nAMD group (r = .42, P = .05). There were no significant differences in mean BAP test results between the nAMD patients and controls for either sex (men: 2241 ± 549 vs. 2136 ± 246 μmol/L; women: 2263 ± 292 vs. 2335 ± 161 μmol/L). CONCLUSION: The d-ROMs test may provide a useful indicator of nAMD in men but not in women.

    DOI: 10.1186/s12886-020-01334-y

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  10. Silicone oil-associated retinal light exposure under a surgical microscope. International journal

    Kazuhisa Yamada, Hiroki Kaneko, Taichi Tsunekawa, Hideyuki Shimizu, Ayana Suzumura, Rina Namba, Jun Takeuchi, Keiko Kataoka, Kei Takayama, Makoto Inoue, Yasuki Ito, Hiroko Terasaki

    Acta ophthalmologica   Vol. 97 ( 5 ) page: E742 - E746   2019.8

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    PURPOSE: Based on the hypothesis that silicone oil (SO) with a higher refractive index than water induces unexpected vignetting effects during surgeries, we aimed to investigate the relationship between the volume of SO and light intensity using a surgical microscope. METHODS: Using a light-sensitive paper and model eye filled with varying volumes (0%, 50%, 62.5%, 75%, 87.5% and 100%) of SO, the light intensity of intraocular lens (IOL) with various refractive powers (0, 10 and 20 dioptres) illuminated by a surgical microscope was measured. RESULTS: Light exposure density with 1.0% coaxial and oblique light was approximately 22-fold higher than that with 0.1% coaxial and oblique light. Further, it was approximately 34-fold higher in eyes with +20 D IOL than in those with no IOL. The density in eyes with 75% SO was the highest among all groups followed by that with 0% SO. Light exposure densities in the eyes with 75% and 0% SO were significantly higher than those with other volumes of SO. In SO-filled eyes, a microscope set with only an oblique light and a filter successfully reduced light exposure. CONCLUSION: Silicone oil-related vision loss (SORVL) during SO removal surgeries might be due to increased light exposure on the macular retina caused by the SO-associated vignetting effect. SORVL could be prevented by placing a filter in the microscope during SO removal surgeries.

    DOI: 10.1111/aos.14038

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  11. Possible mechanism of silicone-oil related vision loss in intractable retinal diseases

    Kaneko Hiroki, Shimizu Hideyuki, Yamada Kazuhisa, Suzumura Ayana, Namba Rina, Terasaki Hiroko

    INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE   Vol. 60 ( 9 )   2019.7

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  12. In Vitro Epiretinal Membrane Model and Antibody Permeability: Relationship With Anti-VEGF Resistance in Diabetic Macular Edema. International journal

    Rina Namba, Hiroki Kaneko, Ayana Suzumura, Hideyuki Shimizu, Keiko Kataoka, Kei Takayama, Kazuhisa Yamada, Yasuhito Funahashi, Seina Ito, Norie Nonobe, Hiroko Terasaki

    Investigative ophthalmology & visual science   Vol. 60 ( 8 ) page: 2942 - 2949   2019.7

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    Purpose: Diabetic macular edema (DME) is characterized by an accumulation of fluid in the macula due to diabetic retinopathy. Currently, anti-VEGF drugs are the standard treatment worldwide for DME. This study aimed to assess whether the existence of epiretinal membrane (ERM) affects anti-VEGF efficacy, due to reduced permeability of the antibody through the ERM. Methods: We retrospectively examined clinical data of DME patients who underwent anti-VEGF treatment and evaluated whether clinical differences existed between DME eyes with ERM and those without ERM. We then created an in vitro ERM model using MIO-M1, ARPE-19, and NTI-4 cells on Transwell membranes and evaluated antibody permeability through this in vitro ERM model using fluorescently labeled antibodies. Results: Central retinal thickness (CRT) change between before and 1 month after first anti-VEGF treatment, as well as final CRT and final visual acuity 12 months after first anti-VEGF treatment, significantly differed between DME eyes with ERM and those without ERM. The in vitro ERM model led to production of collagen I in a manner similar to that of human ERM specimens. Fluorescence intensity of the lower chamber of the in vitro ERM model was significantly reduced in a dose-dependent manner. Conclusions: Clinical data analysis indicated that the existence of ERM in DME eyes lowered the efficacy of anti-VEGF treatment. Reduced antibody permeability through the in vitro ERM model suggested ERM presence was associated with resistance to anti-VEGF treatment in DME eyes with ERM.

    DOI: 10.1167/iovs.19-26788

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  13. OPTICAL COHERENCE TOMOGRAPHY ANGIOGRAPHY OF THE FOVEAL AVASCULAR ZONE IN CHILDREN WITH A HISTORY OF TREATMENT-REQUIRING RETINOPATHY OF PREMATURITY. International journal

    Norie Nonobe, Hiroki Kaneko, Yasuki Ito, Kei Takayama, Keiko Kataoka, Taichi Tsunekawa, Toshiyuki Matsuura, Ayana Suzumura, Hideyuki Shimizu, Hiroko Terasaki

    Retina (Philadelphia, Pa.)   Vol. 39 ( 1 ) page: 111 - 117   2019.1

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    PURPOSE: To examine the characteristics of the foveal vascular structure of patients with retinopathy of prematurity (ROP) by optical coherence tomography angiography. METHODS: Ten patients with a history of laser photocoagulation or cryopexy treatment for Stage 3 (Zone ≥ II) ROP and 10 normal subjects (controls) were included. Foveal avascular zone, vessel density, vessel length, and vascular diameter index were measured by optical coherence tomography angiography using the 3 × 3-mm Early Treatment Diabetic Retinopathy Study (ETDRS) sectors. RESULTS: The median foveal avascular zone values of the patients with ROP and controls were 0.103 mm and 0.260 mm, respectively (P = 0.0025). The medians of the vessel density, vessel length, and vascular diameter index of the patients with ROP were 0.218 mm/mm, 11.75 mm/mm, and 18.00 μm, respectively, in ETDRS Sector 1 and did not significantly differ from those of the controls (P = 0.940, 0.733, and 0.705, respectively). For the average of ETDRS Sectors 2 to 5, the medians of the vessel density, vessel length, and vascular diameter index for the patients with ROP were 0.347 mm/mm, 18.95 mm/mm, and 18.28 μm, respectively; vessel density and vessel length were significantly smaller than those of the controls (P = 0.002 and 0.003, respectively), but there was no significant difference in vascular diameter index (P = 0.286). CONCLUSION: Optical coherence tomography angiography-guided foveal avascular zone was significantly smaller in patients with ROP than in controls. Our results indicate that foveal vascular development may be altered in patients with a history of treatment-requiring ROP.

    DOI: 10.1097/IAE.0000000000001937

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  14. Biological Characteristics of Subsilicone Oil Fluid and Differences With Other Ocular Humors. International journal

    Hideyuki Shimizu, Hiroki Kaneko, Ayana Suzumura, Kei Takayama, Rina Namba, Yasuhito Funahashi, Keiko Kataoka, Takeshi Iwase, Shiang-Jyi Hwang, Seina Ito, Kazuhisa Yamada, Shinji Ueno, Yasuki Ito, Hiroko Terasaki

    Translational vision science & technology   Vol. 8 ( 1 ) page: 28 - 28   2019.1

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    Purpose: Subsilicone oil fluid (SOF) in eyes with silicone oil (SO) endotamponade possibly has a role in complications (e.g., vision loss); thus, we aimed to examine inflammatory cytokine and electrolyte levels and retinal glial cell viability in SOF. Methods: We measured major inflammatory cytokine levels and electrolytes in SOF and compared them with those in vitreous fluid (VF) and anterior chamber fluid (ACF). We analyzed the correlation between inflammatory cytokines and retinal thickness in SO-filled eyes. Further, we measured the MIO-M1 cell viability in medium with SOF and compared it with that containing VF. Results: We collected and examined 57 SOF, 22 ACF, and 21 VF samples from eyes with PVR, PDR, RD, and MH. Interleukin (IL)-8 and monocyte chemoattractant protein (MCP)-1 levels in SOF were significantly higher than those in ACF. There was no significant difference for all cytokines between SOF and VF. Retinal thickness changes during SO endotamponade were not correlated with the presence of any inflammatory cytokines. Levels of ferrous iron, but not of potassium, showed a significant decrease in SOF compared with VF. The WST-1 assay showed that SOF-added medium induced higher MIO-M1 cell viability than VF-added medium. Conclusions: We found no significant correlation between the change in the retinal thickness and cytokine levels, but SOF contains higher concentrations of cytokines and lower concentrations of ferrous iron and can be biologically distinguished from ACF and VF. Translational Relevance: Novel knowledge of inflammatory cytokine levels and electrolytes in SOF provides better understanding of pathology of SO-filled eyes.

    DOI: 10.1167/tvst.8.1.28

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  15. Novel Classification of Early-stage Systemic Hypertensive Changes in Human Retina Based on OCTA Measurement of Choriocapillaris. International journal

    Kei Takayama, Hiroki Kaneko, Yasuki Ito, Keiko Kataoka, Takeshi Iwase, Tetsuhiro Yasuma, Toshiyuki Matsuura, Taichi Tsunekawa, Hideyuki Shimizu, Ayana Suzumura, Eimei Ra, Tomohiko Akahori, Hiroko Terasaki

    Scientific reports   Vol. 8 ( 1 ) page: 15163 - 15163   2018.10

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    The traditional classification of hypertensive retinopathy was based on the Keith-Wagener-Barker (KWB) grading, which is a subjective scaling system, and it is difficult to distinguish between the first and second grades. Retinal and choroidal vasculatures are affected by systemic hypertension, although retinal vasculature changes with age, axial length, intraocular pressure, and retinal diseases. It is necessary to establish a new objective method to assess hypertensive vascular changes. In the present study, we have examined the vasculature of the macular choriocapillaris in order to establish a new objective method to assess hypertensive vascular changes using optical coherence tomography angiography (OCTA). Choriocapillaris vessel density (VD), vessel length, and vessel diameter index in a 3 × 3 mm macular area were measured by OTCA in a total of 567 volunteers (361 healthy subjects and 206 subjects with systemic hypertension) who attended a basic health check-up. Ocular factors, systemic factors, and medications were evaluated. We detected significant differences in normative choriocapillaris vasculature between the left and right eyes in 53 healthy subjects and revealed correlations between age, intraocular pressure, axial length, and choriocapillaris vasculature in 308 healthy subjects. Normative foveal VD was correlated with age only and the efficiency was weak. The analysis of 206 right eyes (KWB grade 0, 159 eyes; grade 1, 35 eyes; and grade 2, 12 eyes) revealed that foveal VD was strongly correlated with KWB grade only (P < 0.001). This is the first report suggesting that OCTA for foveal choriocapillaris measurement by OCTA would might provide the advantage of evaluating be objective method for evaluating the progression of systemic hypertension.

    DOI: 10.1038/s41598-018-33580-y

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  16. The relationship between inflammatory cytokines in the sub-silicone oil fluid and retinal thickness in eyes with proliferative vitreoretinopathy and proliferative diabetic retinopathy.

    Kaneko Hiroki, Shimizu Hideyuki, Tsunekawa Taichi, Iwase Takeshi, Matsuura Toshiyuki, Suzumura Ayana, Namba Rina, Terasaki Hiroko

    INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE   Vol. 59 ( 9 )   2018.7

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  17. Clinical advantage of Swept-Source Optical Coherence Tomography for the patients with retinal hemorrhage due to retinal microaneurysms: a case series

    Shimizu Hideyuki, Kaneko Hiroki, Tsunekawa Taichi, Matsuura Toshiyuki, Suzumura Ayana, Terasaki Hiroko

    INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE   Vol. 59 ( 9 )   2018.7

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  18. Diacron-reactive oxygen metabolites and biological antioxidant potential tests in patients with age-related macular degeneration

    Matsuura Toshiyuki, Kaneko Hiroki, Takayama Kei, Shimizu Hideyuki, Tsunekawa Taichi, Suzumura Ayana, Namba Rina, Terasaki Hiroko

    INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE   Vol. 59 ( 9 )   2018.7

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  19. Optical coherence tomography angiography of choriocapillaris: normative macular choriocapillaris vasculature and novel classification of early-stage systemic hypertensive changes: A cross-sectional study

    Takayama Kei, Ito Yasuki, Kaneko Hiroki, Kataoka Keiko, Iwase Takeshi, Tsunekawa Taichi, Shimizu Hideyuki, Suzumura Ayana, Akahori Tomohiko, Terasaki Hiroko

    INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE   Vol. 59 ( 9 )   2018.7

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  20. The role of brain-derived neurotrophic factor on retinal dysfunction in diabetic retinopathy and its association with eicosapentaenoic acid

    Namba Rina, Kaneko Hiroki, Kataoka Keiko, Tsunekawa Taichi, Matsuura Toshiyuki, Suzumura Ayana, Shimizu Hideyuki, Terasaki Hiroko

    INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE   Vol. 59 ( 9 )   2018.7

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  21. Cytokine profiling in the sub-silicone oil fluid after vitrectomy surgeries for refractory retinal diseases. International journal

    Hiroki Kaneko, Kei Takayama, Tetsu Asami, Yasuki Ito, Taichi Tsunekawa, Takeshi Iwase, Yasuhito Funahashi, Shinji Ueno, Norie Nonobe, Shunsuke Yasuda, Ayana Suzumura, Hideyuki Shimizu, Reona Kimoto, Shiang-Jyi Hwang, Hiroko Terasaki

    Scientific reports   Vol. 7 ( 1 ) page: 2640 - 2640   2017.5

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    Language:Japanese   Publishing type:Research paper (scientific journal)  

    Silicone oil (SO) is an intraocular surgical adjuvant that reduces the surgical complications in refractory retinal diseases, although membrane and cellular proliferation is often seen even in SO-filled eyes. We hypothesised that the fluid in the space between the SO and the retina, named the "sub-silicone oil fluid (SOF)", enhances these biological responses. We proposed a safe method for SOF extraction. We also analysed inflammatory cytokine expressions and SOF osmotic pressures from eyes with rhegmatogenous retinal detachment (RRD), proliferative diabetic retinopathy (PDR), proliferative vitreoretinopathy (PVR) and macular hole-associated retinal detachment (MHRD). Interleukin (IL)-10, IL-12p40, IL-6, monocyte chemotactic protein-1, and vascular endothelial growth factor (VEGF) in the SOF with PVR were significantly higher than in those with RRD or MHRD. Fibroblast growth factor-2, IL-10, IL-12p40, IL-8, VEGF, and transforming growth factor beta 1 levels in eyes with exacerbated PDR indicated a significantly higher expression than those with simple PDR. IL-6 and tumour necrosis factor alpha in eyes with exacerbated PVR demonstrated a significantly higher expression than in those with simple PVR. However, there was no difference in SOF osmotic pressure between group of each disease. These studies indicate that disease-specific SOF is a significant reflection of disease status.

    DOI: 10.1038/s41598-017-03124-x

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  22. Better Visual Outcome by Intraocular Lens Ejection in Geriatric Patients with Ruptured Ocular Injuries. International journal

    Hiroki Kaneko, Tetsu Asami, Tadasu Sugita, Taichi Tsunekawa, Toshiyuki Matsuura, Kei Takayama, Kentaro Yamamoto, Shu Kachi, Yasuki Ito, Shinji Ueno, Norie Nonobe, Keiko Kataoka, Ayana Suzumura, Takeshi Iwase, Hiroko Terasaki

    PloS one   Vol. 12 ( 1 ) page: e0170094   2017.1

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    Language:Japanese   Publishing type:Research paper (scientific journal)  

    Ocular trauma is one of the leading causes of visual impairment worldwide. Because of the popularity of cataract surgeries, aged individuals with ocular trauma commonly have a surgical wound in their eyes. The purpose of this study was to evaluate the visual outcome of cases that were coincident with intraocular lens (IOL) ejection in the eyes with ruptured open-globe ocular injuries. Consecutive patients with open-globe ocular injuries were first reviewed. Patients' characteristics, corrected distance visual acuities (CDVAs) over 3 years after the trauma, causes of injuries, traumatic wound patterns, and coexistence of retinal detachment were examined. The relationships between poor CDVA and the other factors, including the complications of crystalline lens and IOL ejection, were examined. A total of 105 eyes/patients [43 eyes with rupture, 33 with penetrating, 28 with intraocular foreign body (IOFB), and 1 with perforating injuries] were included. Rupture injuries were common in aged patients and were mostly caused by falls, whereas penetrating and IOFB injuries were common in young male patients. CDVAs of the eyes with rupture injuries were significantly worse than those of the eyes with penetrating or IOFB injuries. CDVA from more than 50% of the ruptured eyes resulted in no light perception or light perception to 20/500. CDVA of the ruptured eyes complicated by crystalline lens ejection was significantly worse than that of those complicated by IOL ejection. The wounds of the ruptured eyes complicated by IOL ejection were mainly located at the superior corneoscleral limbus, whereas those of the eyes complicated by crystalline lens ejection were located at the posterior sclera. There were significant correlations between poor CDVA and retinal detachment and crystalline lens ejection. These results proposed a new trend in the ocular injuries that commonly occur in aged patients; history of cataract surgery might affect the final visual outcome after open-globe ocular injuries.

    DOI: 10.1371/journal.pone.0170094

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  23. Correlation between miR-148 Expression in Vitreous and Severity of Rhegmatogenous Retinal Detachment. International journal

    Taichi Tsunekawa, Hiroki Kaneko, Kei Takayama, Shiang-Jyi Hwang, Akio Oishi, Yosuke Nagasaka, Fuxiang Ye, Takeshi Iwase, Norie Nonobe, Shinji Ueno, Yasuki Ito, Shunsuke Yasuda, Toshiyuki Matsuura, Hideyuki Shimizu, Ayana Suzumura, Keiko Kataoka, Hiroko Terasaki

    BioMed research international   Vol. 2017   page: 3427319 - 3427319   2017

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    Language:Japanese   Publishing type:Research paper (scientific journal)  

    Purpose. We had earlier reported positive hsa-miR-148a-3p expression in eyes with rhegmatogenous retinal detachment (RRD) and its involvement in the epithelial-mesenchymal transition of retinal pigment epithelium in vitro. Here we investigated the association of hsa-miR-148a-3p expression levels in the vitreous fluid of patients with RRD with severity of RRD. Methods. The hsa-miR-148a-3p expression levels in the vitreous fluid, range (degree) of retinal detachment (RD), and pixels of retinal break were measured in 27 eyes with RRD. The association of hsa-miR-148a-3p expression levels with other factors was evaluated by multiple regression analysis. Results. The hsa-miR-148a-3p expression levels, time from onset of RRD to vitrectomy, range of RD, and pixels of retinal breaks were 23.68 ± 43.00, 12.07 ± 15.36 days, 155.85 ± 86.67 degrees, and 37000 ± 67100 pixels, respectively. Five eyes with RRD had vitreous hemorrhage preoperatively. The hsa-miR-148a-3p expression levels were significantly associated with pixels of retinal breaks (β = 0.699) and the time from onset of RRD to vitrectomy (β = 0.358) but not with the range of RD or presence of vitreous hemorrhage. Conclusion. The hsa-miR-148a-3p expression levels in the vitreous fluid were significantly associated with the size of retinal break and disease duration.

    DOI: 10.1155/2017/3427319

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MISC 1

  1. 若年者にみられた両眼性の原因不明の網膜浮腫

    鈴村文那、上野真治

    臨床眼科     2022.11

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Presentations 9

  1. 網膜神経保護の視点から考える糖尿病網膜症の早期治療の可能性 Invited

    鈴村文那

    第77回日本臨床眼科学会  2023.10.7 

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    Event date: 2023.10

    Language:Japanese   Presentation type:Symposium, workshop panel (nominated)  

    Venue:東京  

  2. 家族性若年糖尿病として治療中に網膜循環障害が急速に進行した増殖糖尿病網膜症の一例

    鈴村文那

    第29回日本糖尿病眼学会  2023.6.30 

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    Event date: 2023.6 - 2023.7

    Language:Japanese   Presentation type:Poster presentation  

  3. 糖尿病モデルマウスにおける網膜機能障害に対する老化細胞選択除去剤の効果

    第127回日本眼科学会総会 

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    Event date: 2023.4

    Language:Japanese   Presentation type:Oral presentation (general)  

  4. 糖尿病モデルにおけるエイコサペンタエン酸の神経細胞保護効果の検討

    第123回日本眼科学会総会 

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    Event date: 2019.4

    Presentation type:Oral presentation (general)  

  5. 開放性眼外傷の分類毎の特徴と眼球破裂での有水晶体/偽水晶体脱出による術後視力の違い

    第121回日本眼科学会総会 

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    Event date: 2017.4

    Presentation type:Poster presentation  

  6. Neuroprotective roles of eicosapentaenoic acid and its metabolite 18-HEPE via Müller brain-derived neurotrophic factor in diabetic retinopathy International conference

    2021.11.27 

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    Language:English   Presentation type:Oral presentation (general)  

  7. Neuroprotective effect of senolysis in diabetes model mouse Invited International conference

    2023.3.25 

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    Language:English   Presentation type:Oral presentation (general)  

  8. Neuroprotective roles of eicosapentaenoic acid and its metabolite 18-HEPE via Müller brain-derived neurotrophic factor in diabetic retinopathy Invited International conference

    2022.10.22 

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    Language:English   Presentation type:Symposium, workshop panel (nominated)  

  9. The efficacy of intravitreal anti-vascular endothelial growth factor therapy on age-related macular degeneration with asteroid hyalosis International conference

    2017.9.8 

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    Language:English   Presentation type:Oral presentation (general)  

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KAKENHI (Grants-in-Aid for Scientific Research) 1

  1. 老化細胞の選択的除去による糖尿病網膜症の予防と早期治療のための薬剤開発

    Grant number:22K16949  2022.4 - 2025.3

    科学研究費助成事業  若手研究

    鈴村 文那

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    Authorship:Principal investigator 

    Grant amount:\4550000 ( Direct Cost: \3500000 、 Indirect Cost:\1050000 )

    糖尿病の三大合併症の1つである糖尿病網膜症では、不可逆的な網膜内神経細胞の障害が問題となるため、早期からの神経保護治療による“予防的アプローチ”が重要である。
    近年、糖尿病により細胞老化が促進されると共に老化した細胞が糖尿病網膜症の重症化に関与する可能性や、様々なストレスを受けて不可逆的な増殖停止に至った老化細胞を薬剤によって除去できる可能性が示唆されている。
    そこで本研究では老化細胞除去薬に着目し、糖尿病網膜症に対する治療効果とそのメカニズムを検証する。

 

Teaching Experience (On-campus) 1

  1. 眼科

    2022