Updated on 2023/09/14

写真a

 
SAKABE Nanako
 
Organization
Graduate School of Medicine Assistant Professor
Graduate School
Graduate School of Medicine
Undergraduate School
School of Health Sciences
Title
Assistant Professor
Contact information
メールアドレス
External link

Degree 1

  1. 博士(医療技術学) ( 2017.12   名古屋大学 ) 

Research Areas 2

  1. Life Science / Human pathology

  2. Life Science / Medical technology assessment

Research History 2

  1. Nagoya University   Assistant Professor

    2021.4

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    Country:Japan

  2. 株式会社アラクス   研究開発部研究部研究課

    2009.4 - 2021.3

Education 3

  1. Nagoya University

    2011.4 - 2017.3

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    Country: Japan

  2. Nagoya University

    2007.4 - 2009.3

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    Country: Japan

  3. Nagoya University

    2003.4 - 2007.3

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    Country: Japan

Professional Memberships 1

  1. 日本臨床細胞学会

 

Papers 20

  1. Effect of liquid-based cytology fixing solution on immunocytochemistry: Efficacy of antigen retrieval in cytologic specimens. Reviewed International journal

    Nanako Sakabe, Sayumi Maruyama, Chihiro Ito, Yuka Shimoyama, Kazuhisa Sudo, Shouichi Sato, Katsuhide Ikeda

    Diagnostic cytopathology   Vol. 51 ( 9 ) page: 546 - 553   2023.9

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

    BACKGROUND: Immunocytochemistry (ICC) is an indispensable technique to improve diagnostic accuracy. ICC using liquid-based cytology (LBC)-fixed specimens has been reported. However, problems may arise if the samples are not fixed appropriately. We investigated the relationship between the LBC fixing solution and ICC and the usefulness of antigen retrieval (AR) in LBC specimens. METHODS: Specimens were prepared from five types of LBC-fixed samples using cell lines and the SurePath™ method. ICC was performed using 13 antibodies and analyzed by counting the number of positive cells in the immunocytochemically stained specimens. RESULTS: Insufficient reactivity was observed using ICC without heat-induced AR (HIAR) in nuclear antigens. The number of positive cells increased in ICC with HIAR. The percentage of positive cells was lower in CytoRich™ Blue samples for Ki-67 and in CytoRich™ Red and TACAS™ Ruby samples for estrogen receptor and p63 than in the other samples. For cytoplasmic antigens, the percentage of positive cells for no-HIAR treatment specimens was low in the three antibodies used. In cytokeratin 5/6, the number of positive cells increased in all LBC specimens with HIAR, and the percentage of positive cells in CytoRich™ Red and TACAS™ Ruby samples was significantly lower (p < .01). For cell membrane antigens, CytoRich™ Blue samples had a lower percentage of positive cells than the other LBC-fixed samples. CONCLUSION: The combination of detected antigen, used cells, and fixing solution may have different effects on immunoreactivity. ICC using LBC specimens is a useful technique, but the staining conditions should be examined before performing ICC.

    DOI: 10.1002/dc.25178

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  2. Relationship between a deep learning model and liquid-based cytological processing techniques. Reviewed International journal

    Katsuhide Ikeda, Nanako Sakabe, Sayumi Maruyama, Chihiro Ito, Yuka Shimoyama, Wataru Oboshi, Tetsuya Komene, Yoshitaka Yamaguchi, Shouichi Sato, Kohzo Nagata

    Cytopathology : official journal of the British Society for Clinical Cytology   Vol. 34 ( 4 ) page: 308 - 317   2023.7

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    OBJECTIVE: Artificial intelligence (AI)-based cytopathology studies conducted using deep learning have enabled cell detection and classification. Liquid-based cytology (LBC) has facilitated the standardisation of specimen preparation; however, cytomorphology varies according to the LBC processing technique used. In this study, we elucidated the relationship between two LBC techniques and cell detection and classification using a deep learning model. METHODS: Cytological specimens were prepared using the ThinPrep and SurePath methods. The accuracy of cell detection and cell classification was examined using the one- and five-cell models, which were trained with one and five cell types, respectively. RESULTS: When the same LBC processing techniques were used for the training and detection preparations, the cell detection and classification rates were high. The model trained on ThinPrep preparations was more accurate than that trained on SurePath. When the preparation types used for training and detection were different, the accuracy of cell detection and classification was significantly reduced (P < 0.01). The model trained on both ThinPrep and SurePath preparations exhibited slightly reduced cell detection and classification rates but was highly accurate. CONCLUSIONS: For the two LBC processing techniques, cytomorphology varied according to cell type; this difference affects the accuracy of cell detection and classification by deep learning. Therefore, for highly accurate cell detection and classification using AI, the same processing technique must be used for both training and detection. Our assessment also suggests that a deep learning model should be constructed using specimens prepared via a variety of processing techniques to construct a globally applicable AI model.

    DOI: 10.1111/cyt.13235

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  3. Effect of Specimen Processing Technique on Cell Detection and Classification by Artificial Intelligence. Reviewed International journal

    Sayumi Maruyama, Nanako Sakabe, Chihiro Ito, Yuka Shimoyama, Shouichi Sato, Katsuhide Ikeda

    American journal of clinical pathology   Vol. 159 ( 5 ) page: 448 - 454   2023.5

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    OBJECTIVES: Cytomorphology is known to differ depending on the processing technique, and these differences pose a problem for automated diagnosis using deep learning. We examined the as-yet unclarified relationship between cell detection or classification using artificial intelligence (AI) and the AutoSmear (Sakura Finetek Japan) and liquid-based cytology (LBC) processing techniques. METHODS: The "You Only Look Once" (YOLO), version 5x, algorithm was trained on the AutoSmear and LBC preparations of 4 cell lines: lung cancer (LC), cervical cancer (CC), malignant pleural mesothelioma (MM), and esophageal cancer (EC). Detection and classification rates were used to evaluate the accuracy of cell detection. RESULTS: When preparations of the same processing technique were used for training and detection in the 1-cell (1C) model, the AutoSmear model had a higher detection rate than the LBC model. When different processing techniques were used for training and detection, detection rates of LC and CC were significantly lower in the 4-cell (4C) model than in the 1C model, and those of MM and EC were approximately 10% lower in the 4C model. CONCLUSIONS: In AI-based cell detection and classification, attention should be paid to cells whose morphologies change significantly depending on the processing technique, further suggesting the creation of a training model.

    DOI: 10.1093/ajcp/aqac178

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  4. Immunocytochemical Reactivity Comparison between Formalin-Fixed and Liquid-Based Cytology-Fixed Specimens. Reviewed International journal

    Nanako Sakabe, Sayumi Maruyama, Chihiro Ito, Yuka Shimoyama, Tetsuya Komene, Wataru Oboshi, Shouichi Sato, Kazuhisa Sudo, Katsuhide Ikeda

    Acta cytologica   Vol. 67 ( 1 ) page: 38 - 45   2023.1

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

    INTRODUCTION: Liquid-based cytology (LBC)-fixed samples can be used for preparing multiple specimens of the same quality and for immunocytochemistry (ICC); however, LBC fixing solutions affect immunoreactivity. Therefore, in this study, we examined the effect of LBC fixing solutions on immunoreactivity. METHODS: Samples were cell lines, and specimens were prepared from cell blocks of 10% neutral buffered formalin (NBF)-fixed samples and the four types of LBC-fixed samples: PreservCyt®, CytoRich™ Red, CytoRich™ Blue, and TACAS™ Ruby, which were post-fixed with NBF. ICC was performed using 24 different antibodies, and immunocytochemically stained specimens were analyzed for the percentage of positive cells. RESULTS: Immunoreactivity differed according to the type of antigen detected. For nuclear antigens, the highest percentage of positive cells of Ki-67, WT-1, ER, and p63 was observed in the NBF-fixed samples, and the highest percentage of positive cells of p53, TTF-1, and PgR was observed in the TACAS™ Ruby samples. For cytoplasmic antigens, the percentage of positive cells of CK5/6, Vimentin, and IMP3 in LBC-fixed samples was higher than or similar to that in NBF-fixed samples. The percentage of positive cells of CEA was significantly lower in CytoRich™ Red and CytoRich™ Blue samples than in the NBF-fixed sample (p < 0.01). Among the cell membrane antigens, the percentage of positive cells of Ber-EP4, CD10, and D2-40 was the highest in NBF-fixed samples and significantly lower in CytoRich™ Red and CytoRich™ Blue samples than that in NBF-fixed samples (p < 0.01). The NBF-fixed and LBC-fixed samples showed no significant differences in the percentage of positive cells of CA125 and EMA. DISCUSSION/CONCLUSION: ICC using LBC-fixed samples showed the same immunoreactivity as NBF-fixed samples when performed on cell block specimens post-fixed with NBF. The percentage of positive cells increased or decreased based on the type of fixing solution depending on the amount of antigen in the cells. Further, the detection rate of ICC with LBC-fixed samples varied according to the type of antibody and the amount of antigen in the cells. Therefore, we propose that ICC using LBC-fixed samples, including detection methods, should be carefully performed.

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  5. L-ARGININE SUPPLEMENTATION SUBSTANTIALLY ATTENUATES HYPERTENSION BUT NOT CARDIAC INJURY IN RATS WITH METABOLIC SYNDROME

    Tagami Kaito, Okuzawa Touko, Hayakawa Misaki, Cui Xixi, Obara Natsuki, Kunimatsu Asuko, Koide Mayako, Teranishi Tamami, Furukawa Nozomi, Sakabe Nanako, Ikeda Katsuhide, Murohara Toyoaki, Nagata Kohzo

    JOURNAL OF HYPERTENSION   Vol. 41   page: E371 - E371   2023.1

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  6. COMPARATIVE EFFECTS OF RENAL DENERVATION IN DIFFERENT STAGES OF HYPERTENSION ON CARDIAC, RENAL, AND ADIPOSE PATHOLOGY IN DAHL SALT-SENSITIVE RATS

    Nagata Kohzo, Tagami Kaito, Okuzawa Toko, Hayakawa Misaki, Nomura Akane, Nishimura Tomo, Furukawa Nozomi, Sakabe Nanako, Ikeda Katsuhide, Kobuchi Shuhei, Kitada Kento, Fujisawa Yoshihide, Nishiyama Akira, Murohara Toyoaki

    JOURNAL OF HYPERTENSION   Vol. 41   page: E363 - E364   2023.1

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  7. Relationship between Liquid-Based Cytology Preservative Solutions and Artificial Intelligence: Liquid-Based Cytology Specimen Cell Detection Using YOLOv5 Deep Convolutional Neural Network. Reviewed International journal

    Katsuhide Ikeda, Nanako Sakabe, Sayumi Maruyama, Chihiro Ito, Yuka Shimoyama, Shouichi Sato, Kohzo Nagata

    Acta cytologica   Vol. 66 ( 6 ) page: 542 - 550   2022.11

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    INTRODUCTION: Deep learning is a subset of machine learning that has contributed to significant changes in feature extraction and image classification and is being actively researched and developed in the field of cytopathology. Liquid-based cytology (LBC) enables standardized cytological preparation and is also applied to artificial intelligence (AI) research, but cytological features differ depending on the LBC preservative solution types. In this study, the relationship between cell detection by AI and the type of preservative solution used was examined. METHODS: The specimens were prepared from five preservative solutions of LBC and stained using the Papanicolaou method. The YOLOv5 deep convolutional neural network algorithm was used to create a deep learning model for each specimen, and a BRCPT model from five specimens was also created. Each model was compared to the specimen types used for detection. RESULTS: Among the six models, a difference in the detection rate of approximately 25% was observed depending on the detected specimen, and within specimens, a difference in the detection rate of approximately 20% was observed depending on the model. The BRCPT model had little variation in the detection rate depending on the type of the detected specimen. CONCLUSIONS: The same cells were treated with different preservative solutions, the cytologic features were different, and AI clarified the difference in cytologic features depending on the type of solution. The type of preservative solution used for training and detection had an extreme influence on cell detection using AI. Although the accuracy of the deep learning model is important, it is necessary to understand that cell morphology differs depending on the type of preservative solution, which is a factor affecting the detection rate of AI.

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  8. Characterizing the Effect of Processing Technique and Solution Type on Cytomorphology Using Liquid-Based Cytology. Reviewed International journal

    Katsuhide Ikeda, Wataru Oboshi, Yusuke Hashimoto, Tetsuya Komene, Yoshitaka Yamaguchi, Shouichi Sato, Sayumi Maruyama, Nozomi Furukawa, Nanako Sakabe, Kohzo Nagata

    Acta cytologica   Vol. 66 ( 1 ) page: 55 - 60   2022.1

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    INTRODUCTION: Liquid-based cytology (LBC) is increasingly used for nongynecologic applications. However, the cytological preparation of LBC specimens is influenced by the processing technique and the preservative used. In this study, the influence of the processing techniques and preservatives on cell morphology was examined mathematically and statistically. METHODS: Cytological specimens were prepared using the ThinPrep (TP), SurePath (SP), and AutoSmear methods, with 5 different preservative solutions. The cytoplasmic and nuclear areas of Papanicolaou-stained specimens were measured for all samples. RESULTS: The cytoplasmic and nuclear areas were smaller in cells prepared using the 2 LBC methods, compared to that prepared using the AutoSmear method, irrespective of the preservative used. The cytoplasmic and nuclear areas of cells prepared using the SP method were smaller than those of cells prepared using the TP method, irrespective of the preservative used. There were fewer differences among the cytoplasmic areas of cells prepared with different preservative solutions using the TP method; however, the cytoplasmic areas of cells prepared using the SP method changed with the preservative solution used. CONCLUSIONS: The most significant difference affecting the cytoplasmic and nuclear areas was the processing technique. The TP method increased the cytoplasmic and nuclear areas, while the methanol-based PreservCyt solution enabled the highest enlargement of the cell. LBC is a superior preparation technique for standardization of the specimens. Our results offer a better understanding of methods suitable for specimen preparation for developing precision AI-based diagnosis in cytology.

    DOI: 10.1159/000519335

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  9. Pharmacological inhibition of the lipid phosphatase PTEN ameliorates heart damage and adipose tissue inflammation in stressed rats with metabolic syndrome. International journal

    Sao Ashikawa, Yuki Komatsu, Yumeno Kawai, Kiyoshi Aoyama, Shiho Nakano, Xixi Cui, Misaki Hayakawa, Nanako Sakabe, Nozomi Furukawa, Katsuhide Ikeda, Toyoaki Murohara, Kohzo Nagata

    Physiological reports   Vol. 10 ( 1 ) page: e15165   2022.1

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    Phosphatidylinositol 3-kinase (PI3K) signaling promotes the differentiation and proliferation of regulatory B (Breg) cells, and the lipid phosphatase phosphatase and tensin homolog deleted on chromosome 10 (PTEN) antagonizes the PI3K-Akt signaling pathway. We previously demonstrated that cardiac Akt activity is increased and that restraint stress exacerbates hypertension and both heart and adipose tissue (AT) inflammation in DS/obese rats, an animal model of metabolic syndrome (MetS). We here examined the effects of restraint stress and pharmacological inhibition of PTEN on heart and AT pathology in such rats. Nine-week-old animals were treated with the PTEN inhibitor bisperoxovanadium-pic [bpV(pic)] or vehicle in the absence or presence of restraint stress for 4 weeks. BpV(pic) treatment had no effect on body weight or fat mass but attenuated hypertension in DS/obese rats subjected to restraint stress. BpV(pic) ameliorated left ventricular (LV) inflammation, fibrosis, and diastolic dysfunction as well as AT inflammation in the stressed rats. Restraint stress reduced myocardial capillary density, and this effect was prevented by bpV(pic). In addition, bpV(pic) increased the proportions of Breg and B-1 cells as well as reduced those of CD8+ T and B-2 cells in AT of stressed rats. Our results indicate that inhibition of PTEN by bpV(pic) alleviated heart and AT inflammation in stressed rats with MetS. These positive effects of bpV(pic) are likely due, at least in part, to a reduction in blood pressure, an increase in myocardial capillary formation, and an altered distribution of immune cells in fat tissue that result from the activation of PI3K-Akt signaling.

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  10. Effects of neonicotinoid and organophosphorus insecticides on macrophage functions

    Sasou Kanako, Matsushima Miyoko, Ogasawara Nanako, Ohdachi Tomoko, Atsumi Kazuko, Tanaka Kyohka, Sugiyama Tomoshi, Ueyama Jun, Hashimoto Naozumi, Kawabe Tsutomu

    Proceedings for Annual Meeting of The Japanese Pharmacological Society   Vol. WCP2018 ( 0 ) page: PO3-13-24 - 13-24   2018

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    <p>Neonicotinoid insecticides and organophosphorus (OP) insecticides that act on the cholinergic nervous system of insects, are major insecticides used in agriculture throughout the world. The effects of neonicotinoid insecticides and OP insecticides cause overstimulation and desensitization in the nervous system by interacting with the nicotinic acetylcholine receptors (nAChRs) and inhibiting acetylcholine-esterase, respectively. The immunotoxicity of OP insecticides to human has been reported, including exacerbation of inflammation and asthma, and we have reported that diazinon, one of OP insecticides, modulated macrophage functions. Although neonicotinoid insecticides are thought to be relatively low toxic for human compared to OP insecticides due to the low affinity of mammalian nAChRs, less is known about the exact effects of neonicotinoid insecticides. </p><p> In this study, we investigated the effects of neonicotinoid insecticides and OP insecticides on macrophage functions, such as cytokine production, the levels of cell-surface molecule expression, and phagocytosis in RAW264.7 cells. We also investigated the induction of autophagy by neonicotinoid insecticides and OP insecticides.</p>

    DOI: 10.1254/jpssuppl.wcp2018.0_po3-13-24

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  11. Modulation of immunological activity on macrophages induced by diazinon. Reviewed International journal

    Ogasawara N, Matsushima M, Kawamura N, Atsumi K, Yamaguchi T, Ochi H, Kusatsugu Y, Oyabu S, Hashimoto N, Hasegawa Y, Ueyama J, Kawabe T

    Toxicology   Vol. 379   page: 22 - 30   2017.3

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    Diazinon is an organophosphorus (OP) insecticide and is widely used not only in agriculture but also homes and garden in Japan. Diazinon has been reported to increase TNF-α production in rat serum and brain, suggesting that it can modify the proinflammatory response. In this study, we investigated the effects of diazinon on macrophage functions, such as cytokine production, reactive oxygen species (ROS) generation, cyclooxygenase (COX)-2 and inducible nitric oxide synthase (iNOS) expressions, cell-surface molecule expressions, and phagocytosis in RAW264.7 cells. In RAW264.7 cells, diazinon induced the production of TNF-α and IL-6. Diazinon induced ROS generation and the expressions of COX-2, iNOS, and cell-surface molecules CD40, CD86, and MHC class II, but reduced phagocytic activity in RAW264.7 cells. ERK and p38, but not JNK and p65 were involved in diazinon-induced IL-6 expression in RAW264.7 cells. We also examined these proinflammatory responses in bone marrow-derived macrophages (BMDM) and bronchoalveolar lavage fluid (BALF) cells. These results suggested that diazinon can activate macrophages and enhance inflammatory responses.

    DOI: 10.1016/j.tox.2017.01.014

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  12. Involvement of localization changes of caveolin-1 in Nrf2 activation induced by quercetin

    Matsushima Miyoko, Kusatsugu Yuto, Ochi Haruka, Oyabu Savaka, Atsumi Kazuko, Ogasawara Nanako, Kawabe Tsutomu

    JOURNAL OF PHARMACOLOGICAL SCIENCES   Vol. 133 ( 3 ) page: S204 - S204   2017.3

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  13. Critical role of selective autophagy adaptor protein p62 in quercetin-induced Nrf2 activation Reviewed International journal

    Kusatsugu Y., Matsushima M., Oyabu S., Ochi H., Atsumi K., Ogasawara N., Takemura K., Kawabe T.

    EUROPEAN JOURNAL OF IMMUNOLOGY   Vol. 46   page: 437 - 437   2016.8

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  14. Quercetin suppressed imiquimod-induced activation of alveolar macrophages

    Oyabu S., Matsushima M., Omura A., Ogasawara N., Ochi H., Kusatsugu Y., Atsumi K., Takemura K., Kawabe T.

    EUROPEAN JOURNAL OF IMMUNOLOGY   Vol. 46   page: 436 - 436   2016.8

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  15. Involvement of CD40 on immune cells in response to immune complexes

    Atsumi K., Matsushima M., Ogiso H., Ochi H., Kusatsugu Y., Oyabu S., Ogasawara N., Takemura K., Kawabe T.

    EUROPEAN JOURNAL OF IMMUNOLOGY   Vol. 46   page: 1140 - 1141   2016.8

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  16. Involvement of CD40 in the expression of Bcl6

    Ochi H., Matsushima M., Kodera Y., Kusatsugu Y., Atsumi K., Oyabu S., Ogasawara N., Takemura K., Kawabe T.

    EUROPEAN JOURNAL OF IMMUNOLOGY   Vol. 46   page: 1141 - 1141   2016.8

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  17. Involvement of caveolin-1 in quercetin-induced Nrf2 activation

    Matsushima M., Kusatsugu Y., Oyabu S., Ochi H., Atsumi K., Ogasawara N., Takemura K., Kawabe T.

    EUROPEAN JOURNAL OF IMMUNOLOGY   Vol. 46   page: 707 - 708   2016.8

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  18. The Suppressive Effect of Quercetin on Toll-Like Receptor 7-Mediated Activation in Alveolar Macrophages.

    Yasui M, Matsushima M, Omura A, Mori K, Ogasawara N, Kodera Y, Shiga M, Ito K, Kojima S, Kawabe T

    Pharmacology   Vol. 96 ( 5-6 ) page: 201 - 9   2015

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    Respiratory viral infections that cause chronic airway and lung disease can result in the activation of the innate immune response. Alveolar macrophages (AMs), one of the first lines of defense in the lung, are abundantly located in alveoli and the respiratory tract. Flavonoids found in fruits and vegetables exhibit cytoprotective effects on various cell types. In this study, we investigated the effect of quercetin on activation of AMs that had been exposed to imiquimod, a ligand of Toll-like receptor (TLR) 7. In both a mouse AM cell line (AMJ2-C11 cells) and mouse bronchoalveolar fluid cells, we demonstrated that quercetin attenuated TLR7-induced the expression of TNF-α and IL-6. In AMJ2-C11 cells, quercetin also attenuated the TLR7-induced CD40 expression; attenuated the translocation of p65; induced translocation of Nrf2 from cytosol to nucleus; and induced heme oxygenase (HO)-1 expression. Notably, tin protoporphyrin IX (SnPP), an inhibitor of HO-1, also attenuated TLR7-induced transcription of the TNF-α and IL-6 genes, suggesting that the effect of quercetin is mediated by HO-1. These results suggest that dietary supplementation with quercetin may have efficacy in the treatment of respiratory viral infection.

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  19. Hemoglobin induces the expression of indoleamine 2,3-dioxygenase in dendritic cells through the activation of PI3K, PKC, and NF-kappaB and the generation of reactive oxygen species. Reviewed International journal

    Ogasawara N, Oguro T, Sakabe T, Matsushima M, Takikawa O, Isobe K, Nagase F

    Journal of cellular biochemistry   Vol. 108 ( 3 ) page: 716 - 25   2009.10

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    Indoleamine 2,3-dioxygenase (IDO) is the rate-limiting enzyme in the kynurenine (Kyn) pathway of tryptophan (Trp) metabolism. IDO is immunosuppressive and is induced by inflammation in macrophages and dendritic cells (DCs). Previous studies have shown the serum Kyn/Trp levels in patients with hemolytic anemia to be notably high. In the present study, we demonstrated that hemoglobin (Hb), but not hemin or heme-free globin (Apo Hb), induced IDO expression in bone marrow-derived myeloid DCs (BMDCs). Hb induced the phosphorylation and degradation of IκBα. Hb-induced IDO expression was inhibited by inhibitors of PI3-kinase (PI3K), PKC and nuclear factor (NF)-κB. Hb translocated both RelA and p52 from the cytosol to the nucleus and induced the intracellular generation of reactive oxygen species (ROS). Hb-induced IDO expression was inhibited by anti-oxidant N-acetyl-L-cysteine (NAC) or mixtures of SOD and catalase, however, IDO expression was enhanced by 3-amino-1,2,4-triazole, an inhibitor of catalase, suggesting that the generation of ROS such as O2-, H2O2, and hydroxyl radical is required for the induction of IDO expression. The generation of ROS was inhibited by a PKC inhibitor, and this action was further enhanced by addition of a PI3K inhibitor. Hb induced Akt phosphorylation, which was inhibited by a PI3K inhibitor and enhanced by a PKC inhibitor. These results suggest that the activation of NF-κB through the PI3K-PKC-ROS and PI3K-Akt pathways is required for the Hb-induced IDO expression in BMDCs. © 2009 Wiley-Liss, Inc.

    DOI: 10.1002/jcb.22308

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  20. High-affinity uptake of kynurenine and nitric oxide-mediated inhibition of indoleamine 2,3-dioxygenase in bone marrow-derived myeloid dendritic cells.

    Hara T, Ogasawara N, Akimoto H, Takikawa O, Hiramatsu R, Kawabe T, Isobe K, Nagase F

    Immunology letters   Vol. 116 ( 1 ) page: 95 - 102   2008.2

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    Indoleamine 2,3-dioxygenase (IDO)-initiated tryptophan metabolism along the kynurenine (Kyn) pathway in some dendritic cells (DC) such as plasmacytoid DC (pDC) regulates T-cell responses. It is unclear whether bone marrow-derived myeloid DC (BMDC) express functional IDO. The IDO expression was examined in CD11c+CD11b+ BMDC differentiated from mouse bone marrow cells using GM-CSF. CpG oligodeoxynucleotides (CpG) induced the expression of IDO protein with the production of nitric oxide (NO) in BMDC in cultures for 24 h. In the enzyme assay using cellular extracts of BMDC, the IDO activity of BMDC stimulated with CpG was enhanced by the addition of a NO synthase (NOS) inhibitor, suggesting that IDO activity was suppressed by NO production. On the other hand, the concentration of Kyn in the culture supernatant of BMDC was not increased by stimulation with CpG. Exogenously added Kyn was taken up by BMDC independently of CpG stimulation and NO production, and the uptake of Kyn was inhibited by a transport system L-specific inhibitor or high concentrations of tryptophan. The uptake of tryptophan by BMDC was markedly lower than that of Kyn. In conclusion, IDO activity in BMDC is down-regulated by NO production, whereas BMDC strongly take up exogenous Kyn. © 2007 Elsevier B.V. All rights reserved.

    DOI: 10.1016/j.imlet.2007.11.016

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Presentations 25

  1. 細胞処理溶液とAIによる細胞検出の関係性

    下山優香、伊藤千尋、坂部名奈子、池田勝秀

    第64回日本臨床細胞学会総会  2023.6.10 

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    Event date: 2023.6

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:名古屋   Country:Japan  

  2. 細胞標本作製法とディープラーニングモデルの関係性

    下山優香、伊藤千尋、坂部名奈子、池田勝秀

    第64回日本臨床細胞学会総会  2023.6.10 

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    Event date: 2023.6

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:名古屋   Country:Japan  

  3. ダイアジノンは骨髄由来マクロファージを活性化させ、免疫応答を修飾する

    渥美和子、松島充代子、小笠原名奈子、佐宗香奈子、上山 純、川部 勤

    第48回日本職業・環境アレルギー学会総会・学術大会 

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    Event date: 2017.6 - 2017.7

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:AOSSA   Country:Japan  

  4. ケルセチンによるNrf2活性化におけるcaveolin-1の局在変化の関与

    第90回日本薬理学会年会 

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    Event date: 2017.3

    Language:Japanese   Presentation type:Poster presentation  

    Venue:長崎ブリックホール、長崎新聞文化ホール アストピア   Country:Japan  

  5. Involvement of localization changes of caveolin-1 in Nrf2 activation induced by quercetin International conference

    Matsushima Miyoko, Kusatsugu Yuto, Ochi Haruka, Oyabu Savaka, Atsumi Kazuko, Ogasawara Nanako, Kawabe Tsutomu

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    Event date: 2017.3

    Language:Japanese   Presentation type:Poster presentation  

  6. 生体防御機構の誘導を指標とした有害物質の評価法の探索

    大薮沙也果、松島充代子、草次裕人、小笠原名奈子、武村和哉、越智 悠、渥美和子、川部 勤

    第11回臨床検査学教育学会学術大会 

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    Event date: 2016.8 - 2016.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue: 神戸常磐大学   Country:Japan  

  7. Involvement of CD40 in the Expression of Bcl6 International conference

    OCHI Haruka, MATSUSHIMA Miyoko, KODERA Yuka, KUSATSUGU Yuto, OYABU Sayaka, ATSUMI Kazuko, OGASAWARA Nanako, TAKEMURA Kazuya, KAWABE Tsutomu

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    Event date: 2016.8

    Language:English   Presentation type:Poster presentation  

    Country:Australia  

  8. Critical Role of Selective Autophagy Adaptor Protein p62 in Quercetin-Induced Nrf2 Activation International conference

    KUSATSUGU Yuto, MATSUSHIMA Miyoko, OYABU Sayaka, OCHI Haruka, ATSUMI Kazuko, OGASAWARA Nanako, TAKEMURA Kazuya, KAWABE Tsutomu

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    Event date: 2016.8

    Language:English   Presentation type:Poster presentation  

    Country:Australia  

  9. Involvement of Caveolin-1 in Quercetin-Induced Nrf2 Activation International conference

    MATSUSHIMA Miyoko, KUSATSUGU Yuto, OYABU Sayaka, OCHI Haruka, ATSUMI Kazuko, OGASAWARA Nanako, TAKEMURA Kazuya, KAWABE Tsutomu

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    Event date: 2016.8

    Language:English   Presentation type:Poster presentation  

    Country:Australia  

  10. Quercetin Suppressed Imiquimod-Induced Activation of Alveolar Macrophages International conference

    OYABU Sayaka, MATSUSHIMA Miyoko , OMURA Aya, OGASAWARA Nanako, OCHI Haruka, KUSATSUGU Yuto, ATSUMI Kazuko, TAKEMURA Kazuya, KAWABE Tsutomu

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    Event date: 2016.8

    Language:English   Presentation type:Poster presentation  

    Country:Australia  

  11. Involvement of CD40 on Immune Cells in Response to Immune Complexes International conference

    ATSUMI Kazuko, MATSUSHIMA Miyoko, OGISO Hiroki, OCHI Haruka, KUSATSUGU Yuto, OYABU Sayaka, OGASAWARA Nanako, TAKEMURA Kazuya, KAWABE Tsutomu

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    Event date: 2016.8

    Language:English   Presentation type:Poster presentation  

    Country:Australia  

  12. 農薬の環境曝露が免疫機能に及ぼす影響についての基礎的検討

    渥美和子、松島充代子、小笠原名奈子、武村和也、越智 悠、草次裕人、大薮沙也果、上山 純、川部 勤

    第47回日本職業・環境アレルギー学会総会・学術大会 

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    Event date: 2016.7

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:近畿大学11月ホール   Country:Japan  

  13. 有機リン系殺虫剤による免役応答修飾機構についての検討

    松島充代子、小笠原名奈子、河村奈美、山口剛広、高木健三、川部 勤

    第64回日本アレルギー学会学術大会 

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    Event date: 2015.5

    Language:Japanese   Presentation type:Poster presentation  

    Venue:グランドプリンスホテル新高輪国際館パミール   Country:Japan  

  14. 肥満細胞活性化に対するクリシンとケルセチンの抑制効果とその機序の相違

    大村 綾、松島充代子、志賀茉梨化、草次裕人、小笠原名奈子、武村和哉、小寺佑果、小木曽寛希、川部 勤

    第9回日本臨床検査学教育学会学術大会 

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    Event date: 2014.8

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:大田区産業プラザ   Country:Japan  

  15. TLR7を介した肺胞マクロファージの活性化に対するケルセチンの効果

    小寺佑果、松島充代子、小笠原名奈子、武村和哉、志賀茉梨化、大村 綾、小木曽寛希、川部 勤

    第9回日本臨床検査学教育学会学術大会 

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    Event date: 2014.8

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:大田区産業プラザ   Country:Japan  

  16. Inhibitory action of mast cell degranulation by chrysin International conference

    Marika Shiga, Miyoko Matsushima, Yuto Kusatsugu, Nanako Ogasawara, Kazuya Takemura, Yuka Kodera, Aya Oomura, Hiroki Ogiso, Tsutomu Kawabe

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    Event date: 2014.7

    Language:English   Presentation type:Poster presentation  

    Country:Japan  

  17. Effects of quercetin on TLR7-mediated macrophage activation International conference

    Aya Oomura, Miyoko Matsushima, Nanako Ogasawara, Kazuya Takemura, Yuka Kodera, Marika Shiga, Hiroki Ogiso, Tsutomu Kawabe

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    Event date: 2014.7

    Language:English   Presentation type:Poster presentation  

    Country:Japan  

  18. マクロファージの免疫調節機能に及ぼすダイアジノンの効果

    松島充代子、小笠原名奈子、河村奈美、武村和哉、小寺佑果、志賀茉梨花、大村 綾、小木曽寛希、上山 純、川部 勤

    第45回日本職業・環境アレルギー学会総会・学術大会 

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    Event date: 2014.6

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:福岡ソフトリサーチパーク(SRP)センタービル   Country:Japan  

  19. Difference in reaction patterns of M1 and M2 macrophages induced by quercetin International conference

    Miyoko Matsushima, Aya Oomura, Kanae Mori, Nanako Ogasawara, Kazuya Takemura, Haruka Nose, Takehiro Yamaguchi, Yuka Kodera, Marika Shiga, Tsutomu Kawabe

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    Event date: 2013.8

    Language:English   Presentation type:Oral presentation (general)  

    Venue:MiCo Milano Congressi   Country:Italy  

  20. Chrysin regulates mast cell activation via HO-1-independent pathway International conference

    Marika Shiga, Miyoko Matsushima, Ayaka Teranishi, Haruka Nose, Nanako Ogasawara, Kazuya Takemura, Takehiro Yamaguchi, Yuka Kodera, Tsutomu Kawabe

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    Event date: 2013.8

    Language:English   Presentation type:Poster presentation  

    Venue:MiCo Milano Congressi   Country:Italy  

  21. The effect of quercetin on the activated alveolar macrophages International conference

    Haruka Nose, Miyoko Matsushima, Aya Oomura, Kanae Mori, Nanako Ogasawara, Kazuya Takemura, Takehiro Yamaguchi, Yuka Kodera, Marika Shiga, Tsutomu Kawabe

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    Event date: 2013.8

    Language:English   Presentation type:Oral presentation (general)  

    Venue:MiCo Milano Congressi   Country:Italy  

  22. 環境物質に対するフラボノイドの細胞保護効果とその機序

    松島充代子、小笠原名奈子、武村和哉、野瀬遥加、山口剛広、小寺佑果、志賀茉梨花、上山 純、川部 勤

    第44回日本職業・環境アレルギー学会総会・学術大会 

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    Event date: 2013.7

    Language:Japanese   Presentation type:Poster presentation  

    Venue:神奈川   Country:Japan  

  23. 肥満細胞活性化におけるクリシンの効果

    松島充代子、寺西彩香、小笠原名奈子、山本祐規子、高木 健三、川部 勤

    第132回日本薬学会 

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    Event date: 2012.3

    Language:Japanese   Presentation type:Poster presentation  

    Venue:北海道   Country:Japan  

  24. Induction of the indoleamine 2,3-dioxygenase expression in myeloid dendritic cells stimulated with hemoglobin

    小笠原名奈子、小黒崇史、坂部肇胤、松島充代子、長瀬文彦

    第38回日本免疫学会 

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    Event date: 2008.12

    Language:English   Presentation type:Poster presentation  

    Venue:京都   Country:Japan  

  25. ヘモグロビンによるindoleamine 2,3-dioxygenaseの骨髄系樹状細胞における発現の誘導機構

    小笠原名奈子、小黒崇史、坂部肇胤、松島充代子、滝川 修、長瀬文彦

    第38回日本トリプトファン研究会 

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    Event date: 2008.12

    Language:Japanese  

    Venue:岡山   Country:Japan  

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KAKENHI (Grants-in-Aid for Scientific Research) 1

  1. 免疫組織化学の新技法「微粒子標識抗体染色」の確立

    Grant number:22K18227  2022.4 - 2024.3

    日本学術振興会  科学研究費助成事業  若手研究

    坂部 名奈子

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    Authorship:Principal investigator 

    Grant amount:\4680000 ( Direct Cost: \3600000 、 Indirect Cost:\1080000 )

    侵襲性の少ない細胞診検査において、形態像の観察から良悪性を判定するには経験による能力に頼ることが多く、判定に苦慮する際には着色の有無で確認できる酵素抗体法を用いた免疫組織化学が施行される。免疫組織化学は医療や研究分野で必要不可欠な技法であるが、操作が煩雑で時間を要する。また、非特異反応や発色過程での問題点も存在する。本研究では、様々な大きさ・色彩・素材など多岐にわたる性質を持つ微粒子を使用し、発色過程のいらない免疫組織化学の新技法「微粒子標識抗体染色」の確立を目指し、迅速かつ経験・能力に頼らない正確な診断に繋がる試薬を開発する。

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Teaching Experience (On-campus) 6

  1. 病理組織細胞検査学実習A

    2021

  2. 人体構造学実習

    2021

  3. 形態検査技術開発法I

    2021

  4. 自然系基礎科目 生物学実験

    2021

  5. 病態情報科学特論

    2021

  6. 基礎セミナー

    2021

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