Updated on 2022/04/12

写真a

 
HAYASHI Yuki
 
Organization
Graduate School of Science Designated assistant professor
Title
Designated assistant professor

Degree 1

  1. 博士(理学) ( 2014.3   名古屋大学 ) 

 

Papers 5

  1. Molecular basis of plasma membrane H+-ATPase function and potential application in the agricultural production

    Ding Ming, Zhang Maoxing, Zeng Houqing, Hayashi Yuki, Zhu Yiyong, Kinoshita Toshinori

    PLANT PHYSIOLOGY AND BIOCHEMISTRY   Vol. 168   page: 10 - 16   2021.11

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    Language:Japanese   Publisher:Plant Physiology and Biochemistry  

    Increase of crop yield is always the desired goal, manipulation of genes in relation to plant growth is a shortcut to promote crop yield. The plasma membrane (PM) H+-ATPase is the plant master enzyme; the energy yielded by ATP hydrolysis pumps H+ out of cells, establishes the membrane potential, maintains pH homeostasis and provides the proton-motive force required for transmembrane transport of many materials. PM H+-ATPase is involved in root nutrient uptake, epidermal stomatal opening, phloem sucrose loading and unloading, and hypocotyl cell elongation. In this review, we summarize the recent progresses in roles of PM H+-ATPase in nutrient uptake and light-induced stomatal opening and discuss the pivotal role of PM H+-ATPase in crop yield improvement and its potential application in agricultural production by modulating the expression of PM H+-ATPase in crops.

    DOI: 10.1016/j.plaphy.2021.09.036

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  2. Plasma membrane H+-ATPase overexpression increases rice yield via simultaneous enhancement of nutrient uptake and photosynthesis

    Zhang Maoxing, Wang Yin, Chen Xi, Xu Feiyun, Ding Ming, Ye Wenxiu, Kawai Yuya, Toda Yosuke, Hayashi Yuki, Suzuki Takamasa, Zeng Houqing, Xiao Liang, Xiao Xin, Xu Jin, Guo Shiwei, Yan Feng, Shen Qirong, Xu Guohua, Kinoshita Toshinori, Zhu Yiyong

    NATURE COMMUNICATIONS   Vol. 12 ( 1 ) page: 735   2021.2

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    Language:Japanese   Publisher:Nature Communications  

    Nitrogen (N) and carbon (C) are essential elements for plant growth and crop yield. Thus, improved N and C utilisation contributes to agricultural productivity and reduces the need for fertilisation. In the present study, we find that overexpression of a single rice gene, Oryza sativa plasma membrane (PM) H+-ATPase 1 (OSA1), facilitates ammonium absorption and assimilation in roots and enhanced light-induced stomatal opening with higher photosynthesis rate in leaves. As a result, OSA1 overexpression in rice plants causes a 33% increase in grain yield and a 46% increase in N use efficiency overall. As PM H+-ATPase is highly conserved in plants, these findings indicate that the manipulation of PM H+-ATPase could cooperatively improve N and C utilisation, potentially providing a vital tool for food security and sustainable agriculture.

    DOI: 10.1038/s41467-021-20964-4

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  3. Identification of Genes Preferentially Expressed in Stomatal Guard Cells of <i>Arabidopsis thaliana</i> and Involvement of the Aluminum-Activated Malate Transporter 6 Vacuolar Malate Channel in Stomatal Opening.

    Ye W, Koya S, Hayashi Y, Jiang H, Oishi T, Kato K, Fukatsu K, Kinoshita T

    Frontiers in plant science   Vol. 12   page: 744991   2021

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    Language:English   Publisher:Frontiers in Plant Science  

    Stomatal guard cells (GCs) are highly specialized cells that respond to various stimuli, such as blue light (BL) and abscisic acid, for the regulation of stomatal aperture. Many signaling components that are involved in the stomatal movement are preferentially expressed in GCs. In this study, we identified four new such genes in addition to an aluminum-activated malate transporter, ALMT6, and GDSL lipase, Occlusion of Stomatal Pore 1 (OSP1), based on the expression analysis using public resources, reverse transcription PCR, and promoter-driven β-glucuronidase assays. Some null mutants of GC-specific genes evidenced altered stomatal movement. We further investigated the role played by ALMT6, a vacuolar malate channel, in stomatal opening. Epidermal strips from an ALMT6-null mutant exhibited defective stomatal opening induced by BL and fusicoccin, a strong plasma membrane H+-ATPase activator. The deficiency was enhanced when the assay buffer [Cl–] was low, suggesting that malate and/or Cl– facilitate efficient opening. The results indicate that the GC-specific genes are frequently involved in stomatal movement. Further detailed analyses of the hitherto uncharacterized GC-specific genes will provide new insights into stomatal regulation.

    DOI: 10.3389/fpls.2021.744991

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  4. Regulation of stomatal opening and histone modification by photoperiod in Arabidopsis thaliana

    Aoki Saya, Toh Shigeo, Nakamichi Norihito, Hayashi Yuki, Wang Yin, Suzuki Takamasa, Tsuji Hiroyuki, Kinoshita Toshinori

    SCIENTIFIC REPORTS   Vol. 9 ( 1 ) page: 10054   2019.7

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    Language:Japanese   Publisher:Scientific Reports  

    Stomatal movements are regulated by many environmental signals, such as light, CO2, temperature, humidity, and drought. Recently, we showed that photoperiodic flowering components have positive effects on light-induced stomatal opening in Arabidopsis thaliana. In this study, we determined that light-induced stomatal opening and increased stomatal conductance were larger in plants grown under long-day (LD) conditions than in those grown under short-day (SD) conditions. Gene expression analyses using purified guard cell protoplasts revealed that FT and SOC1 expression levels were significantly increased under LD conditions. Interestingly, the enhancement of light-induced stomatal opening and increased SOC1 expression in guard cells due to LD conditions persisted for at least 1 week after plants were transferred to SD conditions. We then investigated histone modification using chromatin immunoprecipitation–PCR, and observed increased trimethylation of lysine 4 on histone 3 (H3K4) around SOC1. We also found that LD-dependent enhancement of light-induced stomatal opening and H3K4 trimethylation in SOC1 were suppressed in the ft-2 mutant. These results indicate that photoperiod is an important environmental cue regulating stomatal opening, and that LD conditions enhance light-induced stomatal opening and epigenetic modification (H3K4 trimethylation) around SOC1, a positive regulator of stomatal opening, in an FT-dependent manner. Thus, this study provides novel insights into stomatal responses to photoperiod.

    DOI: 10.1038/s41598-019-46440-0

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  5. Control of seed dormancy and germination by DOG1-AHG1 PP2C phosphatase complex via binding to heme

    Nishimura Noriyuki, Tsuchiya Wataru, Moresco James J., Hayashi Yuki, Satoh Kouji, Kaiwa Nahomi, Irisa Tomoko, Kinoshita Toshinori, Schroeder Julian I., Yates John R. III, Hirayama Takashi, Yamazaki Toshimasa

    NATURE COMMUNICATIONS   Vol. 9 ( 1 ) page: 2132   2018.6

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    Language:Japanese   Publisher:Nature Communications  

    Abscisic acid (ABA) regulates abiotic stress and developmental responses including regulation of seed dormancy to prevent seeds from germinating under unfavorable environmental conditions. ABA HYPERSENSITIVE GERMINATION1 (AHG1) encoding a type 2C protein phosphatase (PP2C) is a central negative regulator of ABA response in germination; however, the molecular function and regulation of AHG1 remain elusive. Here we report that AHG1 interacts with DELAY OF GERMINATION1 (DOG1), which is a pivotal positive regulator in seed dormancy. DOG1 acts upstream of AHG1 and impairs the PP2C activity of AHG1 in vitro. Furthermore, DOG1 has the ability to bind heme. Binding of DOG1 to AHG1 and heme are independent processes, but both are essential for DOG1 function in vivo. Our study demonstrates that AHG1 and DOG1 constitute an important regulatory system for seed dormancy and germination by integrating multiple environmental signals, in parallel with the PYL/RCAR ABA receptor-mediated regulatory system.

    DOI: 10.1038/s41467-018-04437-9

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Books 2

  1. 植物の通気口「気孔」ー環境に応答する気孔の制御

    林優紀、木下俊則( Role: Joint author)

    2020.9 

  2. 植物の巧みな環境応答 ー植物の情報処理研究の最前線

    木下俊則、林優紀( Role: Joint author)

    2020.9