Updated on 2021/10/21

写真a

 
AOKI Masayo
 
Organization
Graduate School of Medicine Program in Integrated Medicine Social Life Science Designated lecturer
Title
Designated lecturer

Degree 1

  1. 博士(医学) ( 2013.12   日本医科大学 ) 

Research Areas 4

  1. Life Science / Plastic and reconstructive surgery  / Plastic surgery

  2. Life Science / Medical biochemistry  / General medical chemistry

  3. Life Science / Hygiene and public health (non-laboratory)

  4. Life Science / Hygiene and public health (laboratory)

Research History 2

  1. Nagoya University   Graduate School of Medicine

    2020.9

  2. Nippon Medical School Graduate School, Department of Plastic, Reconstructive and Regenerative Surgery

    2016.4 - 2020.8

Education 2

  1. Hirosaki University

  2. 日本医科大学大学院

Awards 1

  1. 日本医科大学医学会奨学賞

    2020.9  

 

Papers 24

  1. Development of an efficient remediation system with a low cost after identification of water pollutants including phenolic compounds in a tannery built-up area in Bangladesh

    Yuan Tian, Tazaki Akira, Hashimoto Kazunori, Al Hossain M. M. Aeorangajeb, Kurniasari Fitri, Ohgami Nobutaka, Aoki Masayo, Ahsan Nazmul, Akhand Anwarul Azim, Kato Masashi

    CHEMOSPHERE   Vol. 280   page: 130959   2021.10

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    Language:Japanese   Publisher:Chemosphere  

    Water pollution caused by tannery wastewater is an important issue in developing countries. Most studies have focused on inorganic chemicals represented by chromium as a tannery-related main pollutant. This is the first study in which pollution of water by tannery-related organic chemicals was assessed by a combination of qualitative and quantitative analyses. Our quantitative analysis showed that the maximum concentration of total phenolic compounds (phenols), consisting of phenol, bisphenol F, p-cresol and chlorocresol, in canal water in a tannery built-up area in Bangladesh was >67-fold higher than the Environmental, Health and Safety (EHS) guideline value. Mapping of our results indicated tanneries as the sources of phenols pollution. Our original depurative, a hydrotalcite-like compound consisting of magnesium and iron (MF-HT), could adsorb all kinds of phenols and exhibited the highest phenol adsorption ability (115.8 mg/g) among reported hydrotalcite-like compounds. The levels of phenols in canal water samples were reduced to levels below the guideline value by using MF-HT with assistance of a photocatalytic reaction. Moreover, the mean level of chromium (112.2 mg/L) in canal water samples was decreased by 99.7% by using the depurative. Thus, the depurative has the potential for solving the problem of tannery-related water pollution by phenols and chromium.

    DOI: 10.1016/j.chemosphere.2021.130959

    Web of Science

    Scopus

    PubMed

  2. Intranasal levels of lead as an exacerbation factor for allergic rhinitis in humans and mice. Reviewed International journal

    Huadong Xu, Nobutaka Ohgami, Masafumi Sakashita, Kazuhiro Ogi, Kazunori Hashimoto, Akira Tazaki, Keming Tong, Masayo Aoki, Shigeharu Fujieda, Masashi Kato

    The Journal of Allergy and Clinical Immunology   Vol. 148 ( 1 ) page: 139 - +   2021.7

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    Language:Japanese   Publishing type:Research paper (scientific journal)  

    BACKGROUND: Air pollutants are suspected to affect pathological conditions of allergic rhinitis (AR). OBJECTIVES: After detecting Pb (375 μg/kg) in Japanese cedar pollen, the effects of intranasal exposure to Pb on symptoms of AR were investigated. METHODS: Pollen counts, subjective symptoms, and Pb levels in nasal epithelial lining fluid (ELF) were investigated in 44 patients with Japanese cedar pollinosis and 57 controls from preseason to season. Effects of intranasal exposure to Pb on symptoms were confirmed by using a mouse model of AR. RESULTS: Pb levels in ELF from patients were >40% higher than those in ELF from control subjects during the pollen season but not before the pollen season. Pb level in ELF was positively associated with pollen counts for the latest 4 days before visiting a hospital as well as scores of subjective symptoms. Intranasal exposure to Pb exacerbated symptoms in allergic mice, suggesting Pb as an exacerbation factor. Pb levels in ELF and nasal mucosa in Pb-exposed allergic mice were higher than those in Pb-exposed nonallergic mice, despite intranasally challenging the same amount of Pb. Because the increased Pb level in the nasal mucosa of Pb-exposed allergic mice was decreased after washing the nasal cavity, Pb on the surface of but not inside the nasal mucosa may have been a source of increased Pb level in ELF of allergic mice. CONCLUSIONS: Increased nasal Pb level partially derived from pollen could exacerbate subjective symptoms of AR, indicating Pb as a novel hazardous air pollutant for AR.

    DOI: 10.1016/j.jaci.2021.03.019

    Web of Science

    Scopus

    PubMed

  3. The Latest Strategy for Keloid and Hypertrophic Scar Prevention and Treatment: The Nippon Medical School (NMS) Protocol. Reviewed

    Rei Ogawa, Teruyuki Dohi, Mamiko Tosa, Masayo Aoki, Satoshi Akaishi

    Journal of Nippon Medical School   Vol. 88 ( 1 ) page: 2 - 9   2021.3

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    Language:English   Publishing type:Research paper (scientific journal)  

    In 2006, we established a scar/keloid-specialized institution in the Department of Plastic, Reconstructive, and Aesthetic Surgery at Nippon Medical School (NMS) in Tokyo, Japan. In the ensuing 15 years, we treated approximately 2000 new scar/keloid patients annually. This extensive experience has greatly improved the efficacy of the treatments we offer. Therefore, we discuss here the latest NMS protocol for preventing and treating keloids and hypertrophic scars. While this protocol was optimized for Japanese patients, our experiences with a growing body of international patients suggest that it is also effective in other ethnicities. The extensive evidence-based experience underlying the NMS protocol suggests that it may be suitable as the foundation of a standard international prevention/treatment algorithm for pathological scars.

    DOI: 10.1272/jnms.JNMS.2021_88-106

    PubMed

  4. Combination Therapy Composed of Surgery, Postoperative Radiotherapy, and Wound Self-management for Umbilical Keloids. Reviewed International journal

    Teruyuki Dohi, Shigehiko Kuribayashi, Masayo Aoki, Mamiko Tosa, Satoshi Akaishi, Rei Ogawa

    Plastic and Reconstructive Surgery-Global Open   Vol. 8 ( 10 ) page: e3181   2020.10

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    Language:English   Publishing type:Research paper (scientific journal)  

    Background: A universally accepted therapeutic strategy for umbilical keloids has not been determined. Our team has had considerable success with combination therapy composed of surgical excision followed by postoperative radiotherapy and steroid plaster/injection. Methods: All consecutive patients with umbilical keloids that developed from endoscopic surgical scars and underwent minimal-margin keloid excision followed by umbilicoplasty with a flap if needed, tension-reduction suturing, and postoperative radiotherapy in 2013-2017 in the keloid/scar-specialized clinic at the Department of Plastic, Reconstructive and Aesthetic Surgery of Nippon Medical School. The postsurgical radiotherapy regimen was 15 Gy administered in 2 fractions over 2 consecutive days. Radiotherapy was followed by tension-reducing wound self-management with silicone tape or, if needed, steroid plaster. The primary study focus was keloid recurrence during the 24-month follow-up period. Recurrence was defined as the growth of stiff red lesions in even small areas of the scar that was refractory to 2-6 months of steroid-plaster therapy. Results: The case series consisted of 34 patients with 34 lesions. Three lesions (8.8%) recurred. One recurrence was successfully treated by concomitant steroid plaster/injection. The other 2 cases were resistant to steroid injection and underwent reoperation without radiotherapy followed by 6 months of steroid-plaster therapy. None of the 3 cases recurred within 2 years of steroid plaster/injection completion or reoperation. Conclusion: Umbilical keloids can be successfully treated by customized treatment plans that involve appropriate surgical modalities (including umbilicoplasty, if required), postoperative radiotherapy (15 Gy/2 fractions/2 days), and wound/scar self-management with silicone tape and steroid plaster.

    DOI: 10.1097/GOX.0000000000003181

    PubMed

  5. Direct Delivery of Apatite Nanoparticle-Encapsulated siRNA Targeting TIMP-1 for Intractable Abnormal Scars. Reviewed International journal

    Masayo Aoki, Noriko M Matsumoto, Teruyuki Dohi, Hiroaki Kuwahawa, Satoshi Akaishi, Yuri Okubo, Rei Ogawa, Hirofumi Yamamoto, Kazuaki Takabe

    Molecular Therapy Nucleic Acids   Vol. 22   page: 50 - 61   2020.8

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

    Hypertrophic scars (HSs) and keloids are histologically characterized by excessive extracellular matrix (ECM) deposition. ECM deposition depends on the balance between matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteases (TIMPs). TIMP-1 has been linked to ECM degradation and is therefore a promising therapeutic strategy. In this study, we generated super carbonate apatite (sCA) nanoparticle-encapsulated TIMP-1 small interfering RNA (siRNA) (siTIMP1) preparations and examined the effect of local injections on mouse HSs and on ex vivo-cultured keloids. The sCA-siTIMP1 injections significantly reduced scar formation, scar cross-sectional areas, collagen densities, and collagen types I and III levels in the lesions. None of the mice died or exhibited abnormal endpoints. Apatite accumulation was not detected in the other organs. In an ex vivo keloid tissue culture system, sCA-siTIMP1 injections reduced the thickness and complexity of collagen bundles. Our results showed that topical sCA-siTIMP1 injections during mechanical stress-induced HS development reduced scar size. When keloids were injected three times with sCA-siTIMP1 during 6 days, keloidal collagen levels decreased substantially. Accordingly, sCA-siRNA delivery may be an effective approach for keloid treatment, and further investigations are needed to enable its practical use.

    DOI: 10.1016/j.omtn.2020.08.005

    PubMed

  6. Geometric modeling and a retrospective cohort study on the usefulness of fascial tensile reductions in severe keloid surgery. Reviewed International journal

    Takuya Tsuge, Masayo Aoki, Satoshi Akaishi, Teruyuki Dohi, Hiroya Yamamoto, Rei Ogawa

    Surgery   Vol. 167 ( 2 ) page: 504 - 509   2020.2

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    Authorship:Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

    BACKGROUND: Severe keloids are currently treated with surgical resection followed by radiation. Radiotherapy is essential for preventing recurrences. Fascia tensile reduction suturing may also prevent recurrence. We asked whether superficial fascia tensile reduction with or without deep fascia tensile reduction reduced skin mechanical tension and yielded good outcomes. METHODS: Geometric modeling on 3-dimensional anatomic shapes assessed the effect of superficial fascia tensile reduction with or without deep fascia tensile reduction on skin tension. A retrospective cohort study was performed on patients with severe anterior-chest keloids with Japan Scar Workshop-scar scale classification score ≥ 16 who underwent resection plus fascia tensile reduction plus radiotherapy between 2011 and 2016 and were followed for >18 months. Patient characteristics and 18-month postoperative outcomes were examined. Postoperative outcome was defined as rates of keloid disappearance, improvement, and obvious recurrence. RESULTS: Maximal mechanical forces placed on the dermis by dermal sutures, dermal sutures plus superficial fascia tensile reduction, and dermal sutures plus superficial fascia tensile reduction plus deep fascia tensile reduction were 4,700, 573, and 697 Pa, respectively. Adding deep fascia tensile reduction to superficial fascia tensile reduction decreased the force on the superficial fascia. Of 77 cohort patients, 27 and 50 underwent superficial fascia tensile reduction and superficial fascia tensile reduction plus deep fascia tensile reduction, respectively. Superficial fascia tensile reduction plus deep fascia tensile reduction patients underwent complete excision more often (60.0% vs 37.0%, P = .046). The groups did not differ in 18-month surgical outcome, including recurrence rate (P = .670). CONCLUSION: Our 2003 study showed that in anterior-chest keloids, resection plus non-fascial suturing plus radiotherapy led to a 43.1% recurrence. Thus, fascia tensile reduction suturing helps reduce anterior-chest keloid recurrence to ∼5.2%. Superficial fascia tensile reduction plus deep fascia tensile reduction is suitable for relatively large keloids that require total resection. Deep fascia tensile reduction may facilitate superficial fascia tensile reduction but may only be useful when it is technically difficult to achieve reduction with superficial fascia tensile reduction alone.

    DOI: 10.1016/j.surg.2019.07.028

    PubMed

  7. Gene Expression Profile of Isolated Dermal Vascular Endothelial Cells in Keloids. Reviewed International journal

    Noriko M Matsumoto, Masayo Aoki, Yuri Okubo, Kosuke Kuwahara, Shigeyoshi Eura, Teruyuki Dohi, Satoshi Akaishi, Rei Ogawa

    Frontiers in Cell and Developmental Biology   Vol. 8   page: 658 - 658   2020

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    Authorship:Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

    Wound healing is a complex biological process, and imbalances of various substances in the wound environment may prolong healing and lead to excessive scarring. Keloid is abnormal proliferation of scar tissue beyond the original wound margins with excessive deposition of extracellular matrix (ECM) and chronic inflammation. Despite numerous previous research efforts, the pathogenesis of keloid remains unknown. Vascular endothelial cells (VECs) are a major type of inductive cell in inflammation and fibrosis. Despite several studies on vascular morphology in keloid formation, there has been no functional analysis of the role of VECs. In the present study, we isolated living VECs from keloid tissues and investigated gene expression patterns using microarray analysis. We obtained 5 keloid tissue samples and 6 normal skin samples from patients without keloid. Immediately after excision, tissue samples were gently minced and living cells were isolated. Magnetic-activated cell sorting of VECs was performed by negative selection of fibroblasts and CD45+ cells and by positive selection of CD31+cells. After RNA extraction, gene expression analysis was performed to compare VECs isolated from keloid tissue (KVECs) with VECs from normal skin (NVECs). After cell isolation, the percentage of CD31+ cells as measured by flow cytometry ranged from 81.8%-98.6%. Principal component analysis was used to identify distinct molecular phenotypes in KVECs versus NVECs and these were divided into two subgroups. In total, 15 genes were upregulated, and 3 genes were downregulated in KVECs compared with NVECs using the t-test (< 0.05). Quantitative RT-PCR and immunohistochemistry showed 16-fold and 11-fold overexpression of SERPINA3 and LAMC2, respectively. SERPINA3 encodes the serine protease inhibitor, α1-antichymotripsin. Laminin γ2-Chain (LAMC2) is a subunit of laminin-5 that induces retraction of vascular endothelial cells and enhances vascular permeability. This is the first report of VEC isolation and gene expression analysis in keloid tissue. Our data suggest that SERPINA3 and LAMC2 upregulation in KVECs may contribute to the development of fibrosis and prolonged inflammation in keloid. Further functional investigation of these genes will help clarify the mechanisms of abnormal scar tissue proliferation.

    DOI: 10.3389/fcell.2020.00658

    PubMed

  8. The Immunosuppressant Fingolimod (FTY720) for the Treatment of Mechanical Force-Induced Abnormal Scars. Reviewed International journal

    Masayo Aoki, Akatsuki Kondo, Noriko Matsunaga, Azusa Honda, Yuri Okubo, Kazuaki Takabe, Rei Ogawa

    Journal of Immunology Research   Vol. 2020   page: 7057195 - 7057195   2020

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

    Aim: Abnormal scars such as hypertrophic scars (HSs) and keloids are excessively growing scars that exhibit chronic inflammation and capillary vasculogenesis. The lipid mediator sphingosine-1-phosphate (S1P) is important in inflammatory cell recruitment and angiogenesis. Fingolimod (FTY720) is an analog of S1P and thus functionally antagonizes S1P receptors and inhibits the enzyme that produces S1P. We examined the effects of topical FTY720 injections on mechanical force-induced HS progression. Methods: Mechanical force-induced HSs were generated in C57BL6/J mice by suturing a dorsal incision and applying a stretching device on Days 6, 8, 10, and 12. On Days 8, 10, and 12, intracutaneous FTY720 (10 μM) or control vehicle injections were performed. On Day 14, scar tissues and blood were procured and subjected to histology and flow cytometry. Results: Flow cytometry showed that FTY720 decreased the frequencies of macrophages with M2 predominance in the scars but had no effect on total, CD4+, or CD8a+ T cell frequencies. FTY720 also decreased the vascular endothelial cell frequencies in the scar along with the microvessels, as determined by immunohistochemistry. Compared to the vehicles, FTY720 treatment significantly reduced the gross scar area and the cross-sectional scar area on histology. On the other hand, FTY720 tended to reduce white blood cells and significantly reduced the lymphocyte frequencies in the blood. Conclusion: Topical FTY720 induces M2 predominance and impairs angiogenesis. Therefore, its local immunosuppressive mechanisms differ from those of conventional immunosuppressive agents. Topical FTY720 can be a novel therapeutic option for abnormal scars that are difficult to control with corticosteroids. Its lymphocytopenic effects may be limited by careful optimization of the treatment regimen.

    DOI: 10.1155/2020/7057195

    PubMed

  9. Z-plasty and Postoperative Radiotherapy for Upper-arm Keloids: An Analysis of 38 Patients. Reviewed International journal

    Teruyuki Dohi, Shigehiko Kuribayashi, Mamiko Tosa, Masayo Aoki, Satoshi Akaishi, Rei Ogawa

    Plastic and Reconstructive Surgery-Global Open   Vol. 7 ( 11 ) page: e2496   2019.11

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    Language:English   Publishing type:Research paper (scientific journal)  

    Therapies for upper arm keloids include surgical excision followed by postoperative radiotherapy, silicone tape stabilization, and steroid plaster. However, a universally accepted therapeutic strategy for upper-arm keloids is lacking. Methods: All consecutive patients with single upper-arm keloids who underwent keloid excision followed by tension-reducing suturing, multiple z-plasties, and postoperative radiotherapy in 2013-2016 in the keloid/scar specialist clinic at the Department of Plastic, Reconstructive and Aesthetic Surgery of Nippon Medical School, were included in this case series study. Only keloids that arose from the small injury produced during Bacillus Calmette-Guérin vaccination were selected. The postsurgical radiotherapy regimen was 18 Gy administered in 3 fractions over 3 days. Radiotherapy was followed by tension-reducing wound self-management with silicone tape and, if needed, steroid plaster. The primary study objective was keloid recurrence during the 24-month follow-up period. Recurrence was defined as the growth of stiff red lesions in even small areas of the scar that was refractory to at least 2 months of steroid plaster therapy. Results: In total, 38 patients with 38 lesions were enrolled. Two lesions (5.3%) recurred. Both recurrences were successfully treated by concomitant steroid plaster and steroid injection. The recurrence patients were significantly more likely than the nonrecurrence patients to have multiple keloids. The 2 groups did not differ in terms of original keloid size. Conclusions: Upper-arm keloids can be successfully treated by customized plans that involve appropriate surgical modalities (including multiple z-plasties), postoperative radiotherapy (18 Gy/3 fractions/3 d), and postoperative wound/scar self-management with silicone tape and steroid plaster.

    DOI: 10.1097/GOX.0000000000002496

    PubMed

  10. Sphingosine-1-Phosphate Facilitates Skin Wound Healing by Increasing Angiogenesis and Inflammatory Cell Recruitment with Less Scar Formation. Reviewed International journal

    Masayo Aoki, Hiroaki Aoki, Partha Mukhopadhyay, Takuya Tsuge, Hirofumi Yamamoto, Noriko M Matsumoto, Eri Toyohara, Yuri Okubo, Rei Ogawa, Kazuaki Takabe

    International Journal of Molecular Sciences   Vol. 20 ( 14 )   2019.7

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    Authorship:Lead author   Language:English  

    Wound healing starts with the recruitment of inflammatory cells that secrete wound-related factors. This step is followed by fibroblast activation and tissue construction. Sphingosine-1-phosphate (S1P) is a lipid mediator that promotes angiogenesis, cell proliferation, and attracts immune cells. We investigated the roles of S1P in skin wound healing by altering the expression of its biogenic enzyme, sphingosine kinase-1 (SphK1). The murine excisional wound splinting model was used. Sphingosine kinase-1 (SphK1) was highly expressed in murine wounds and that SphK1-/- mice exhibit delayed wound closure along with less angiogenesis and inflammatory cell recruitment. Nanoparticle-mediated topical SphK1 overexpression accelerated wound closure, which associated with increased angiogenesis, inflammatory cell recruitment, and various wound-related factors. The SphK1 overexpression also led to less scarring, and the interaction between transforming growth factor (TGF)-β1 and S1P receptor-2 (S1PR2) signaling is likely to play a key role. In summary, SphK1 play important roles to strengthen immunity, and contributes early wound healing with suppressed scarring. S1P can be a novel therapeutic molecule with anti-scarring effect in surgical, trauma, and chronic wound management.

    DOI: 10.3390/ijms20143381

    PubMed

  11. Cytochrome P450 genes play central roles in transcriptional response by keratinocytes to a high-voltage alternating current electric field. Reviewed International journal

    Masayo Aoki, Noriko M Matsumoto, Yuri Okubo, Rei Ogawa

    Bioelectrochemistry   Vol. 126   page: 163 - 171   2019.4

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    The endogenous electric field (EF) of skin wounds plays an important role in the biological processes that underlie wound healing. Treatments that modulate wound-EFs promote healing. However, the mechanism(s) that underlie this effect remain unclear. Agilent-based microarrays were used to determine the transcriptomes of the keratinocyte line HaCaT, normal human dermal fibroblasts, and the human dermal endothelial cell line HMEC-1 before and after high-voltage alternating current (AC)-EF (14,000 V, 90 Hz) treatment. The keratinocytes had the most genes whose transcription was altered by EF. They included the cytochrome P450 (CYP) genes CYP1A1 and CYP1B1, HMOX1, EREG, DUSP5, and SLC7A11 (all upregulated), and DOCK8, ABCC6, and CYP26A1 (all downregulated). As shown by transcriptional-network analysis, all three CYP genes played central roles in the EF-induced changes in keratinocyte transcriptome. To the best of our knowledge, this is the first study that demonstrates that CYP genes play a key role in the transcriptional responses of human keratinocytes to EF treatment. Further investigations into the effects of EF on wound healing, aging, and regenerative medicine are likely to yield promising results.

    DOI: 10.1016/j.bioelechem.2018.11.014

    PubMed

  12. The Roles of Sphingosine Kinases in Skin Aging. Reviewed International journal

    Masayo Aoki, Hiroaki Aoki, Partha Mukhopadhyay, Eriko Katsuta, Kazuaki Takabe

    Journal of Investigative Dermatology   Vol. 139 ( 4 ) page: 951 - 953   2019.4

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    Authorship:Lead author   Language:English  

    DOI: 10.1016/j.jid.2018.06.192

    PubMed

  13. Histological analysis of hyalinised keloidal collagen formation in earlobe keloids over time: collagen hyalinisation starts in the perivascular area. Reviewed International journal

    Noriko M Matsumoto, Wei-Xia Peng, Masayo Aoki, Satoshi Akaishi, Ryuji Ohashi, Rei Ogawa, Zenya Naito

    International Wound Journal   Vol. 14 ( 6 ) page: 1088 - 1093   2017.12

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    Keloids grow and do not regress. They are characterised histologically by hyalinised keloidal collagen (HKC). HKC amounts vary, and the mechanism by which they form is unclear. To clarify how HKCs form and whether their formation associates with specific clinical features, we studied the histological findings of earlobe keloids and compared them with respective clinical features. A total of 50 earlobe keloids from 43 patients were used for histological analysis of keloid size (mm2 ), HKC area (mm2 ) and HKC area ratio (%). As a result, keloid durations ranged from 3 months to >13 years. Early-stage keloids exhibited little HKC and a tendency for the HKCs to locate in perivascular regions. In later-stage keloids, the HKCs were extremely interconnected and formed a thick bitten donut-shaped region. HKC area ratios correlated positively with keloid duration (r2 = 0·58, P<0·05). HKC area ratios and keloid durations did not correlate with keloid sizes. These patterns of HKC formation and growth may explain why local therapies, which effectively remove fibroblasts and accumulated collagen but not HKCs, are ineffective in older keloids. Keloids should be promptly treated after diagnosis, and older keloids with extensive HKCs may require surgical excision followed by radiotherapy.

    DOI: 10.1111/iwj.12763

    PubMed

  14. 【創傷治癒のトピックス-第5回世界創傷治癒学会連合会議-】 耳垂ケロイド組織における硝子化した膠原線維の病理組織学的検討

    松本典子, 青木雅代, 赤石諭史, 小川令

    形成外科   Vol. 60 ( 11 ) page: 1256 - 1263   2017.11

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    Language:Japanese  

  15. 【創傷治癒のトピックス:第5回世界創傷治癒学会連合会議】ケロイド・肥厚性瘢痕の病態とその最新治療:ケロイド・肥厚性瘢痕は治療できる

    小川令, 赤石諭史, 青木雅代, 土肥輝之, 渡邉真泉, 有馬樹里, 松本典子, 野一色千景

    形成外科   Vol. 60 ( 11 ) page: 1240 - 1245   2017.11

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  16. Periauricular Keloids on Face-Lift Scars in a Patient with Facial Nerve Paralysis. Reviewed International journal

    Masayo Aoki, Satoshi Akaishi, Noriko M Matsumoto, Takuya Tsuge, Ken Kubomura, Midori Nishikawa, Shunichi Nomoto, Rei Ogawa

    Plastic and Reconstructive Surgery-Global Open   Vol. 5 ( 7 ) page: e1417   2017.7

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    Keloids are caused by excessive scar formation that leads to scar growth beyond the initial scar boundaries. Keloid formation and progression is promoted by mechanical stress such as skin stretch force. Consequently, keloids rarely occur in paralyzed areas and areas with little skin tension, such as the periauricular region. Therefore, periauricular incision is commonly performed for face lifts. We report a rare case of keloids that arose from face-lift scars in a patient with bilateral facial nerve paralysis. A 51-year-old Japanese man presented with abnormal proliferative skin masses in bilateral periauricular scars. Seventeen years before, he had a cerebral infarction that resulted in permanent bilateral facial nerve paralysis. Three years before presentation, the patient underwent face-lift surgery with periauricular incisions. We diagnosed multiple keloids. We removed the masses surgically, closed the wounds with sutures in the superficial musculoaponeurotic system layer to reduce tension on the wound edges, reconstructed the earlobes with local skin flaps, and provided 2 consecutive days of radiotherapy. The wounds/scars were managed with steroid plasters and injections. Histology confirmed that the lesions were keloids. Ten months after surgery, the lesions did not exhibit marked regrowth. The keloids appeared to be caused by the patient's helmet, worn during his 3-hour daily motorcycle rides, which placed repeated tension on the periauricular area. This rare case illustrates how physical force contributes to auricular and periauricular keloid development and progression. It also shows that when performing surgery with periauricular incisions, care should be taken to eliminate wound/scar stretching.

    DOI: 10.1097/GOX.0000000000001417

    PubMed

  17. Experimental Rat Skin Flap Model That Distinguishes between Venous Congestion and Arterial Ischemia: The Reverse U-Shaped Bipedicled Superficial Inferior Epigastric Artery and Venous System Flap. Reviewed International journal

    Noriko M Matsumoto, Masayo Aoki, Junichi Nakao, Wei-Xia Peng, Yoshihiro Takami, Hiroki Umezawa, Satoshi Akaishi, Ryuji Ohashi, Zenya Naito, Rei Ogawa

    Plastic and reconstructive surgery   Vol. 139 ( 1 ) page: 79e - 84e   2017.1

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    BACKGROUND: The commonly used flap models have drawbacks that limit their usefulness. In the random skin flap model, flap necrosis is caused by both arterial and venous insufficiency. In the axial skin flap model, flap viability is easily affected by the pedicle blood flow and can result in complete necrosis. This study aimed to establish a new rat skin flap model that has a consistent flap survival rate and in which venous congestion and arterial ischemia can be readily distinguished macroscopically. METHODS: Rats underwent reverse U-shaped bipedicled superficial epigastric artery flap elevation. The right superficial epigastric vessels formed the pedicle. In the control rats (n = 3), the left superficial epigastric vessels were left intact. In the ischemia group (n = 10), the left superficial epigastric artery was ligated. In the congestion group (n = 10), the left superficial epigastric vein was ligated. The flap was returned to the original site and sutured. The surrounding neovascularization was blocked by polyurethane film. Flap survival rates were evaluated on postoperative day 3. RESULTS: The flaps in the ischemia and congestion groups were noticeably pale and violet, respectively. Flap necrosis was noted in the contralateral distal zone only. It started on postoperative day 2 in the ischemia and congestion groups. The mean flap survival rates of the control, ischemia, and congestion groups were 100 percent, 61.8 percent (range, 56.9 to 67.1 percent), and 42.3 percent (35.7 to 48.7 percent), respectively (all p < 0.001). CONCLUSIONS: The flap facilitated discrimination of the effects of ischemia and congestion. This new rat skin flap model is simple and easy to construct, and has a consistent flap survival rate.

    DOI: 10.1097/PRS.0000000000002900

    PubMed

  18. Murine model of long-term obstructive jaundice. Reviewed

    Aoki H, Aoki M, Yang J, Katsuta E, Mukhopadhyay P, Ramanathan R, Woelfel IA, Wang X, Spiegel S, Zhou H, Takabe K.

    Journal of Surgical Research   Vol. 206 ( 1 ) page: 118 - 125   2016

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  19. Sphingosine-1-phosphate signaling in immune cells and inflammation: roles and therapeutic potential. Invited Reviewed

    Aoki M, Aoki H, Ramanathan R, Hait NC, Takabe K.

    Mediators of Inflammation   Vol. 2016   page: 8606878   2016

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  20. Objective spectrometric measurement of keloid color in the east Asian population: pitfalls of subjective color measurements. Reviewed

    Aoki M, Akaishi S, Nakao J, Dohi T, Hyakusoku H, Ogawa R.

    Journal of Nippon Medical School   Vol. 83 ( 4 ) page: 142 - 149   2016

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  21. Tissue inhibitor of metalloproteinase-2 suppresses collagen synthesis in cultured keloid fibroblasts. Reviewed

    Dohi T, Miyake K, Aoki M, Ogawa R, Akaishi S, Shimada T, Okada T, Hyakusoku H.

    Plastic and Reconstructive Surgery. Global Open     2015

  22. siRNA knockdown of tissue inhibitor of metalloproteinase-1 in keloid fibroblasts leads to degradation of collagen type I. Reviewed

    Aoki M, Miyake K, Ogawa R, Dohi T, Akaishi S, Hyakusoku H, Shimada T.

    Journal of Investigative Dermatology   Vol. 134 ( 3 ) page: 818 - 826   2014

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  23. Chest wall reconstruction of severe mediastinitis with intercostal artery-based pedicled vertical rectus abdominis muscle flap with oblique-designed skin pedicle. Reviewed

    Nguyen DT, Aoki M, Hyakusoku H, Ogawa R.

    Annals of Plastic Surgery   Vol. 67 ( 3 ) page: 269 - 271   2011

  24. Clinical applications of basic research that shows reducing skin tension could prevent and treat abnormal scarring: the importance of fascial/subcutaneous tensile reduction sutures and flap surgery for keloid and hypertrophic scar reconstruction. Reviewed

    Ogawa R, Akaishi S, Huang C, Dohi T, Aoki M, Omori Y, Koike S, Kobe K, Akimoto M, Hyakusoku H.

    Journal of Nippon Medical School   Vol. 78 ( 2 ) page: 68 - 76   2011

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Presentations 15

  1. 耳垂ケロイド組織における血管,リンパ管形成に関する免疫組織化学的検討

    本田梓, 松本典子, 青木雅代, 小川令

    日本形成外科学会基礎学術集会(第26回)  2017.10 

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    Language:Japanese  

    Venue:大阪  

  2. 耳垂ケロイドの鑑別疾患に関する検討

    加来知恵美, 松本典子, 安齋眞一, 青木雅代, 赤石諭史, 小川令

    谷根千形成懇話会(第16回)  2017.7 

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    Language:Japanese  

    Venue:東京  

  3. 微細針を用いた手技により発生した異常瘢痕の2例

    野田良博, 青木雅代, 本田梓, 西川みどり, 赤石諭史, 小川令

    瘢痕・ケロイド治療研究会(第12回)  2017.11 

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    Language:Japanese  

    Venue:京都  

  4. マウス肥厚性瘢痕モデルにおけるフィンゴリモド(FTY720)の瘢痕抑制効果(第1報)

    松永宜子, 青木雅代, 本田梓, 大久保ゆり, 赤石諭史, 小川令

    日本創傷治癒学会(第47回)  2017.11 

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    Language:Japanese  

    Venue:京都  

  5. マウス肥厚性瘢痕モデルにおけるフィンゴリモド(FTY720)の瘢痕抑制効果

    松永宜子, 青木雅代, 本田梓, 野本俊一, 小川令

    谷根千形成懇話会(第16回)  2017.7 

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    Language:Japanese  

    Venue:東京  

  6. マウス肥厚性瘢痕モデルにおけるフィンゴリモド(FTY720)の瘢痕抑制効果

    松永宜子, 青木雅代, 本田梓, 野本俊一, 小川令

    日本医科大学医学会総会(第85回)  2017.9 

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    Language:Japanese  

    Venue:東京  

  7. ケロイド・肥厚性瘢痕の病態解明に向けて

    小川令, 赤石諭史, 青木雅代, 土肥輝之, 渡邉真泉, 有馬樹里, 松本典子, 野一色千景

    日本医科大学医学会総会(第85回)  2017.9 

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    Language:Japanese  

    Venue:東京  

  8. ケロイドに類似したAtypical intradermal smooth muscle neoplasmの1例

    本田梓, 青木雅代, 野田良博, 西川みどり, 野本俊一, 赤石諭史, 小川令

    瘢痕・ケロイド治療研究会(第12回)  2017.11 

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    Language:Japanese  

    Venue:京都  

  9. ケロイドに特徴的な硝子化した膠原線維形成における血管関連因子の検討

    野田良博, 松本典子, 青木雅代, 小川令

    日本形成外科学会基礎学術集会(第26回)  2017.10 

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    Language:Japanese  

    Venue:大阪  

  10. ケロイドとの鑑別に苦慮した皮膚平滑筋腫の1例

    野田良博, 青木雅代, 本田梓, 西川みどり, 野本俊一, 赤石諭史, 小川令

    瘢痕・ケロイド治療研究会(第12回)  2017.11 

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    Language:Japanese  

    Venue:京都  

  11. Topical Application of Sphingosine-1-Phosphate Promotes Deep Dermal Burn Healing International conference

    Tsuge T, Aoki M, Kubomura K, Akaishi S, Takabe K, Ogawa R

    Annual Academic Surgical Congress(The 12th)  2017.2 

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    Language:English  

    Venue:Las Vegas  

  12. Differential diagnosis of earlobe keloid

    Matsumoto M, Ansai S, Aoki M, Ogawa R

    日本美容外科学会(JSAPS)学術集会(第128回)  2017.1 

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    Language:English  

    Venue:東京  

  13. 耳垂ケロイド組織における血管,リンパ管形成に関する免疫組織化学的検討

    本田梓, 松本典子, 青木雅代, 小川令

    日本医科大学医学会総会(第85回)  2017.9 

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    Language:Japanese  

    Venue:東京  

  14. 高電圧電界治療に対するヒト皮膚由来細胞の転写応答

    青木雅代, 松本典子, 小川令

    日本形成外科学会基礎学術集会(第26回)  2017.10 

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    Language:Japanese  

    Venue:大阪  

  15. 耳垂腫瘤の鑑別診断

    加来知恵美, 松本典子, 青木雅代, 赤石諭史, 安齋眞一, 小川令

    日本医科大学医学会総会(第85回)  2017.9 

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    Language:Japanese  

    Venue:東京  

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KAKENHI (Grants-in-Aid for Scientific Research) 5

  1. 糖尿病性潰瘍の予防と早期治療に関する新規応用研究

    Grant number:21K09762  2021.4 - 2024.3

    科学研究費助成事業  基盤研究(C)

    青木 雅代, 田崎 啓

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    Authorship:Principal investigator 

    Grant amount:\4160000 ( Direct Cost: \3200000 、 Indirect Cost:\960000 )

    糖尿病性潰瘍・壊疽の患者数は増加しており、生命予後は不良で、医療経済や医療・介護従事者の負担は増している。進行すると治癒は極めて困難であるため、再生医療や細胞治療などの研究が進んでいるが、適応は限定的である。一方、予防や早期治療に関する研究はあまり進んでおらず、現状ではフットケアのみが推進されている。生命予後、医療経済や医療・介護従事者の負担を考慮すると、予防と早期リカバリーは最も重要な課題である。
    末梢神経の機能改善(末梢神経障害による潰瘍の予防)、血流促進(末梢動脈疾患による潰瘍の予防・早期回復)、組織障害の進行抑制(壊疽への進行抑制)を目的に、スフィンゴシン-1-リン酸の有用性を検討する。

  2. ケロイド線維芽細胞におけるlncRNAの機能解析

    Grant number:18K17005  2018.4 - 2020.3

    科学研究費助成事業  若手研究

    青木 雅代

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    Authorship:Principal investigator 

    Grant amount:\4160000 ( Direct Cost: \3200000 、 Indirect Cost:\960000 )

    ヒト正常皮膚およびケロイドから、初代線維芽細胞を培養した。マイクロアレイ法を用いて、遺伝子発現解析とlncRNA解析を網羅的に行った (n=2)。細胞外マトリックス分解酵素であるマトリックスメタロプロテアーゼ (MMP)-1, 3, 7, 10が発現低下を示しており、線維化の進行に関与すると考えられた。これらのMMPsはすべて、遺伝子マップ上、染色体11q22.2に位置している。そこで、染色体11q22.2付近に位置するlncRNAに絞りこんで解析を行った。正常皮膚線維芽細胞と比較して、ケロイドで発現変動の大きいlncRNAを抽出したところ、H19とLINC00900がそれぞれ23倍、7倍の発現上昇を示した。そこで、これら2つのlncRNAの線維化における役割に絞りこんで、調べていくことにした。現在、サンプル数を増やしてマイクロアレイ結果の検証を行うために、引き続き初代線維芽細胞を培養している。今後、遺伝子導入実験、遺伝子抑制実験により、これら2つのlncRNAの新たな機能を探っていく。
    今年度は、ターゲットlncRNAを絞り込むところまでを目標としていたため、おおむね順調に進展していると考えている。
    まず、サンプル数を増やし、定量的RT-PCRにてマイクロアレイ結果の検証を行う。
    ターゲットlncRNAの、ケロイドにおける発現変動が証明されれば、発現プラスミド構築へと進む。発現プラスミドを用いて、遺伝子導入実験を行う。ヒト正常皮膚線維芽細胞に過剰発現させることで、MMPsの発現変動を見る。また、MMPsの転写因子であるAP-1を介して調節している可能性が高いため、AP-1の抑制実験も組み合わせて検証していく。次に、ケロイド由来線維芽細胞を用いる。ターゲットlncRNAをsiRNAで抑制し、MMPsの発現が上昇するかを検証する。

  3. ケロイド線維芽細胞におけるlncRNAの機能解析

    2018.4 - 2020.3

    Nippon Medical School  Grant-in-Aid for Early-Career Scientists

    青木 雅代

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    Authorship:Principal investigator  Grant type:Competitive

    Grant amount:\4160000 ( Direct Cost: \3200000 、 Indirect Cost:\960000 )

  4. スーパーカーボネートアパタイトを用いたケロイド・肥厚性瘢痕の核酸外用薬治療の確立

    2017

    科学研究費補助金 若手研究(B) 

    青木雅代

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    Authorship:Principal investigator  Grant type:Competitive

    Grant amount:\1200000

  5. Development of novel siRNA treatment using super carbonate apatite for keloid/hypertrophic scar

    Grant number:16K20371  2016.4 - 2018.3

    Grants-in-Aid for Scientific Research  Grant-in-Aid for Young Scientists (B)

    Aoki Masayo, MATSUMOTO M Noriko, TAKABE Kazuaki, OGAWA Rei

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    Authorship:Principal investigator 

    Grant amount:\3900000 ( Direct Cost: \3000000 、 Indirect Cost:\900000 )

    We investigated the effect of novel siRNA therapy using mouse hypertrophic scar model. siTIMP1 (siRNA that suppresses the expression of TIMP-1 (a factor involved in excessive collagen deposition)) was capsuled in nanoparticles; super carbonate apatites (sCA) and injected.
    Local injection of siControl(siCON)-sCA or siTIMP1-sCA were performed. The scar sizes were significantly smaller in the scars treated with siTIMP1 than those treated with siCON. Inhibited protein expressions of collagen type 1 and 3 were observed in the scars treated with siTIMP1. siTIMP1-sCA is promising as a novel therapeutic strategy for keloids and hypertrophic scars.