研究者詳細 - 萩尾　華子

Different ways of evolving tool-using brains in teleosts and amniotes 査読有り国際共著国際誌

Pierre Estienne, Matthieu Simion, Hanako Hagio, Naoyuki Yamamoto , Arnim Jenett, Kei Yamamoto

Communications Biology 7 巻頁： 88 2024年1月

Optogenetic manipulation of Gq- and Gi/o-coupled receptor signaling in neurons and heart muscle cells 査読有り国際共著国際誌

Hagio Hanako, Koyama Wataru, Hosaka Shiori, Song Aysenur Deniz, Narantsatsral Janchiv, Matsuda Koji, Sugihara Tomohiro, Shimizu Takashi, Koyanagi Mitsumasa, Terakita Akihisa, Hibi Masahiko, Jesuthasan Suresh

eLife 12 巻 2023年8月

担当区分：筆頭著者記述言語：英語出版者・発行元：eLife

G-protein-coupled receptors (GPCRs) transmit signals into cells depending on the G protein type. To analyze the functions of GPCR signaling, we assessed the effectiveness of animal G-protein-coupled bistable rhodopsins that can be controlled into active and inactive states by light application using zebrafish. We expressed Gq- and Gi/o-coupled bistable rhodopsins in hindbrain reticulospinal V2a neurons, which are involved in locomotion, or in cardiomyocytes. Light stimulation of the reticulospinal V2a neurons expressing Gq-coupled spider Rh1 resulted in an increase in the intracellular Ca2+ level and evoked swimming behavior. Light stimulation of cardiomyocytes expressing the Gi/o-coupled mosquito Opn3, pufferfish TMT opsin, or lamprey parapinopsin induced cardiac arrest, and the effect was suppressed by treatment with pertussis toxin or barium, suggesting that Gi/o-dependent regulation of inward-rectifier K+ channels controls cardiac function. These data indicate that these rhodopsins are useful for optogenetic control of GPCR-mediated signaling in zebrafish neurons and cardiomyocytes.

DOI： 10.7554/eLife.83974

Optogenetic manipulation of neuronal and cardiomyocyte functions in zebrafish using microbial rhodopsins and adenylyl cyclases 査読有り国際共著国際誌

Hagio Hanako, Koyama Wataru, Hosaka Shiori, Song Aysenur Deniz, Narantsatsral Janchiv, Matsuda Koji, Shimizu Takashi, Hososhima Shoko, Tsunoda Satoshi P., Kandori Hideki, Hibi Masahiko

eLife 12 巻 2023年8月

担当区分：筆頭著者記述言語：英語出版者・発行元：eLife

Even though microbial photosensitive proteins have been used for optogenetics, their use should be optimized to precisely control cell and tissue functions in vivo. We exploited GtCCR4 and KnChR, cation channelrhodopsins from algae, BeGC1, a guanylyl cyclase rhodopsin from a fungus, and photoactivated adenylyl cyclases (PACs) from cyanobacteria (OaPAC) or bacteria (bPAC), to control cell functions in zebrafish. Optical activation of GtCCR4 and KnChR in the hindbrain reticulospinal V2a neurons, which are involved in locomotion, induced swimming behavior at relatively short latencies, whereas activation of BeGC1 or PACs achieved it at long latencies. Activation of GtCCR4 and KnChR in cardiomyocytes induced cardiac arrest, whereas activation of bPAC gradually induced bradycardia. KnChR activation led to an increase in intracellular Ca2+ in the heart, suggesting that depolarization caused cardiac arrest. These data suggest that these optogenetic tools can be used to reveal the function and regulation of zebrafish neurons and cardiomyocytes.

DOI： 10.7554/eLife.83975

Distribution of pufferfish saxitoxin- and tetrodotoxin-binding protein homolog and tetrodotoxin in the brain and pituitary of juvenile tiger puffer Takifugu rubripes 査読有り

Ascending Visual Pathways to the Telencephalon in Teleosts with Special Focus on Forebrain Visual Centers, Associated Neural Circuitries, and Evolution 招待有り査読有り国際誌

Hanako Hagio, Naoyuki Yamamoto

Zoological Science 40 巻頁： 105 - 118 2023年3月

Afferent and efferent connections of the nucleus prethalamicus in the yellowfin goby Acanthogobius flavimanus 査読有り国際誌

Hanako Hagio, Masahumi Kawaguchi, Hideki Abe, Naoyuki Yamamoto

Journal of Comparative Neurology 529 巻 ( 1 ) 頁： 87 - 110 2021年1月

Non-thalamic origin of zebrafish sensory nuclei implies convergent evolution of visual pathways in amniotes and teleosts 査読有り国際共著国際誌

Solal Bloch§, Hanako Hagio§, Manon Thomas, Aurélie Heuzé, Jean-Michel Hermel, Elodie Lasserre, Ingrid Colin, Kimiko Saka, Pierre Affaticati, Arnim Jenett, Koichi Kawakami, Naoyuki Yamamoto, Kei Yamamoto

eLife 9 巻頁： 1 - 27 2020年9月

Amano, M; Amiya, N; Otsuka, Y; Homma, J; Hagio, H; Takatani, T; Yamamoto, N; Yamaguchi, K; Sakakura, Y

TOXICON 246 巻頁： 107777 - 107777 2024年8月

掲載種別：研究論文（学術雑誌）出版者・発行元：Toxicon

Pufferfish saxitoxin- and tetrodotoxin (TTX)-binding protein (PSTBP) is considered to transfer TTX between tissues. The immunohistochemical distribution of PSTBP-homolog (PSTBPh) and TTX in the brain and pituitary of hatchery-reared juvenile tiger puffer Takifugu rubripes was investigated. PSTBPh was observed mainly in the pars intermedia of the pituitary. TTX was only detected in a TTX-fed fish in the neurohypophysis of the pituitary and in several other brain regions. The relationship between PSTBPh and TTX is discussed.

DOI： 10.1016/j.toxicon.2024.107777

Morphological analysis of the cerebellum and its efferent system in a basal actinopterygian fish, Polypterus senegalus 査読有り国際誌

Ikenaga T, Shimomai R, Hagio H, Kimura S, Matsumoto K, Kato D, Uesugi K, Takeuchi A, Yamamoto N, Hibi M

Journal of Comparative Neurology 530 巻頁： 1231 - 1246 2022年6月

Multiple gonadotropin-releasing hormone systems in non-mammalian vertebrates: Ontogeny, anatomy, and physiology 招待有り査読有り国際共著国際誌

Satoshi Ogawa, Naoyuki Yamamoto, Hanako Hagio, Yoshitaka Oka, Ishwar S. Parhar

Journal of Neuroendocrinology 頁： e13068 2022年5月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Journal of Neuroendocrinology

Three paralogous genes for gonadotropin-releasing hormone (GnRH; gnrh1, gnrh2, and gnrh3) and GnRH receptors exist in non-mammalian vertebrates. However, there are some vertebrate species in which one or two of these paralogous genes have become non-functional during evolution. The developmental migration of GnRH neurons in the brain is evolutionarily conserved in mammals, reptiles, birds, amphibians, and jawed teleost fish. The three GnRH paralogs have specific expression patterns in the brain and originate from multiple sites. In acanthopterygian teleosts (medaka, cichlid, etc.), the preoptic area (POA)-GnRH1 and terminal nerve (TN)-GnRH3 neuronal types originate from the olfactory regions. In other fish species (zebrafish, goldfish and salmon) with only two GnRH paralogs (GnRH2 and GnRH3), the TN- and POA-GnRH3 neuronal types share the same olfactory origin. However, the developmental origin of midbrain (MB)-GnRH2 neurons is debatable between mesencephalic or neural crest site. Each GnRH system has distinctive anatomical and physiological characteristics, and functions differently. The POA-GnRH1 neurons are hypophysiotropic in nature and function in the neuroendocrine control of reproduction. The non-hypophysiotropic GnRH2/GnRH3 neurons probably play neuromodulatory roles in metabolism (MB-GnRH2) and the control of motivational state for sexual behavior (TN-GnRH3).

DOI： 10.1111/jne.13068