Updated on 2021/10/20

写真a

 
BESSHO Manabu
 
Organization
Institute for Advanced Research Designated assistant professor
Graduate School of Science Designated assistant professor
Title
Designated assistant professor
Contact information
メールアドレス
Other name(s)
Manabu Bessho-Uehara
External link

Degree 1

  1. 博士(農学)

Research Interests 7

  1. Evolution

  2. kleptoprotein

  3. Bioluminescence

  4. Octocorallia

  5. Luciferin

  6. Ctenophora

  7. Parapriacanthus ransonneti

Research Areas 1

  1. Life Science / Evolutionary biology  / Bioluminescence

Research History 3

  1. Nagoya University   Institute for Advanced Research   Designated assistant professor

    2020.4

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    Country:Japan

  2. Monterey Bay Aquarium Research Institute   Research and Development   Postdoctoral fellow

    2018.4 - 2020.3

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    Country:United States

  3. Chubu University   College of Bioscience and Biotechnology   Researcher

    2017.9 - 2018.3

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    Country:Japan

Education 1

  1. Nagoya University   Graduate School of Bioagricultural Sciences

    2014.4 - 2017.3

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    Country: Japan

    Notes: PhD student

Committee Memberships 1

  1. The Rising Generation of Evo-Devo Biologists   Organizing committee member of  

    2014 - 2017   

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    Committee type:Academic society

 

Papers 8

  1. Evidence for de novo Biosynthesis of the Luminous Substrate Coelenterazine in Ctenophores Reviewed International coauthorship International journal

    Manabu Bessho-Uehara, Wentao Huang, Wyatt L. Patry, William E. Browne, Jing-Ke Weng, Steven H.D. Haddock

    iScience   Vol. 23 ( 12 ) page: 101859 - 101859   2020.12

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    Authorship:Lead author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:Elsevier BV  

    DOI: 10.1016/j.isci.2020.101859

    Scopus

    PubMed

  2. Biochemical characterization of diverse deep-sea anthozoan bioluminescence systems Reviewed International coauthorship International journal

    Manabu Bessho-Uehara, Warren R. Francis, Steven H. D. Haddock

    Marine Biology   Vol. 167 ( 8 )   2020.8

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:Springer Science and Business Media LLC  

    DOI: 10.1007/s00227-020-03706-w

    Other Link: http://link.springer.com/article/10.1007/s00227-020-03706-w/fulltext.html

  3. Kleptoprotein bioluminescence: Parapriacanthus fish obtain luciferase from ostracod prey Invited Reviewed International coauthorship International journal

    Manabu Bessho-Uehara, Naoyuki Yamamoto, Shuji Shigenobu, Hitoshi Mori, Keiko Kuwata, Yuichi Oba

    Science Advances   Vol. 6 ( 2 ) page: eaax4942 - eaax4942   2020.1

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:American Association for the Advancement of Science (AAAS)  

    Through their diet, animals can obtain substances essential for imparting special characteristics, such as toxins in monarch butterflies and luminescent substances in jellyfishes. These substances are typically small molecules because they are less likely to be digested and may be hard for the consumer to biosynthesize. Here, we report that <italic>Parapriacanthus ransonneti</italic>, a bioluminescent fish, obtains not only its luciferin but also its luciferase enzyme from bioluminescent ostracod prey. The enzyme purified from the fish’s light organs was identical to the luciferase of <italic>Cypridina noctiluca</italic>, a bioluminescent ostracod that they feed upon. Experiments where fish were fed with a related ostracod, <italic>Vargula hilgendorfii</italic>, demonstrated the specific uptake of the luciferase to the fish’s light organs. This “kleptoprotein” system allows an organism to use novel functional proteins that are not encoded in its genome and provides an evolutionary alternative to DNA-based molecular evolution.

    DOI: 10.1126/sciadv.aax4942

    Web of Science

    PubMed

  4. Firefly genomes illuminate parallel origins of bioluminescence in beetles Reviewed International coauthorship International journal

    Timothy R. Fallon, Sarah E. Lower, Ching-Ho Chang, Manabu Bessho-Uehara, Gavin J. Martin, Adam J. Bewick, Megan Behringer, Humberto J. Debat, Isaac Wong, John C. Day, Anton Suvorov, Christian J. Silva, Kathrin F. Stanger-Hall, David W. Hall, Robert J. Schmitz, David R. Nelson, Sara M. Lewis, Shuji Shigenobu, Seth M. Bybee, Amanda M. Larracuente, Yuichi Oba, Jing-Ke Weng

    ELIFE   Vol. 7   2018.10

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:ELIFE SCIENCES PUBLICATIONS LTD  

    Fireflies and their luminous courtships have inspired centuries of scientific study. Today firefly luciferase is widely used in biotechnology, but the evolutionary origin of bioluminescence within beetles remains unclear. To shed light on this long-standing question, we sequenced the genomes of two firefly species that diverged over 100 million-years-ago: the North American Photinus pyralis and Japanese Aquatica lateralis. To compare bioluminescent origins, we also sequenced the genome of a related click beetle, the Caribbean Ignelater luminosus, with bioluminescent biochemistry near-identical to fireflies, but anatomically unique light organs, suggesting the intriguing hypothesis of parallel gains of bioluminescence. Our analyses support independent gains of bioluminescence in fireflies and click beetles, and provide new insights into the genes, chemical defenses, and symbionts that evolved alongside their luminous lifestyle.

    DOI: 10.7554/eLife.36495

    Web of Science

    PubMed

  5. 19. Structure and Biological Activity of Blarina Paralytic Peptides (BPPs) from the Short-tailed Shrew <i>Blarina brevicauda</i> Invited Reviewed International coauthorship International journal

    Kita Masaki, Takenaka Toshiko, Bessho–Uehara Manabu, Maturana Andres D., Kigoshi Hideo, Ohdachi Satoshi D., Uemura Daisuke

    Symposium on the Chemistry of Natural Products, symposium papers   Vol. 60 ( 0 ) page: 109 - 114   2018

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    Authorship:Lead author   Language:Japanese   Publishing type:Research paper (scientific journal)   Publisher:Symposium on the Chemistry of Natural Products Steering Committee  

    DOI: 10.24496/tennenyuki.60.0_109-114

    CiNii Article

  6. Identification and characterization of the Luc2-type luciferase in the Japanese firefly, Luciola parvula, involved in a dim luminescence in immobile stages Reviewed International coauthorship International journal

    Manabu Bessho-Uehara, Yuichi Oba

    LUMINESCENCE   Vol. 32 ( 6 ) page: 924 - 931   2017.9

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:WILEY  

    Nocturnal Japanese fireflies, Luciola parvula, emit from their lanterns a yellow light, one of the most red-shifted colors found among fireflies. Previously, we isolated and characterized two different types of luciferase gene, Luc1 and Luc2, from the fireflies Luciola cruciata and Luciola lateralis; Luc1 is responsible for the green-yellow luminescence of larval and adult lanterns, whereas Luc2 is responsible for the dim greenish glow of eggs and pupal bodies. The biological role of firefly lanterns in adults is related to sexual communication, but why the eggs and pupae glow remains uncertain. In this study, we isolated the gene Luc2 from L. parvula, and compared its expression profiles and enzymatic characteristics with those of Luc1. A semi-quantitative reverse transcription polymerase chain reaction showed that Luc1 was predominantly expressed in larvae, prepupae, pupae and adults, whereas Luc2 was expressed in eggs, prepupae, pupae and adult females. Enzymatic analyses showed that the luminescent color of Luc1 matches the visual sensitivity of L. parvula eyes, whereas that of Luc2 is very different from it. These results suggest that the biological role of Luc2 expressed in immobile stages is not intraspecific communication.

    DOI: 10.1002/bio.3273

    Web of Science

    PubMed

  7. Biochemical characteristics and gene expression profiles of two paralogous luciferases from the Japanese firefly Pyrocoelia atripennis (Coleoptera, Lampyridae, Lampyrinae): insight into the evolution of firefly luciferase genes Reviewed International coauthorship International journal

    Manabu Bessho-Uehara, Kaori Konishi, Yuichi Oba

    PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES   Vol. 16 ( 8 ) page: 1301 - 1310   2017.8

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:ROYAL SOC CHEMISTRY  

    Two paralogous genes of firefly luciferase, Luc1 and Luc2, have been isolated from the species in two subfamilies, Luciolinae and Photurinae, of the family Lampyridae. The gene expression profiles have previously been examined only in the species of Luciolinae. Here we isolated Luc1 and Luc2 genes from the Japanese firefly Pyrocoelia atripennis. This is the first report of the presence of both Luc1 and Luc2 genes in the species of the subfamily Lampyrinae and of the exon-intron structure of Luc2 in the family Lampyridae. The luminescence of both gene products peaked at 547 nm under neutral buffer conditions, and the spectrum of Luc1, but not Luc2, was red-shifted under acidic conditions, as observed for Luc2 in the Luciolinae species. The semi-quantitative reverse transcription-polymerase chain reaction suggested that Luc1 was expressed in lanterns of all the stages except eggs, while Luc2 was expressed in the non-lantern bodies of eggs, prepupae, pupae, and female adults. These expression profiles are consistent with those in the Luciolinae species. Considering the distant phylogenetic relationship between Lampyrinae and Luciolinae in Lampyridae, we propose that fireflies generally possess two different luciferase genes and the biochemical properties and gene expression profiles for each paralog are conserved among lampyrid species.

    DOI: 10.1039/c7pp00110j

    Web of Science

    Scopus

    PubMed

  8. Biochemical characteristics and gene expression profiles of two paralogous luciferases from the Japanese firefly Pyrocoelia atripennis (Coleoptera, Lampyridae, Lampyrinae): Insight into the evolution of firefly luciferase genes Reviewed

    Manabu Bessho-Uehara

    Photochemical and Photobiological Sciences   Vol. 16   page: 1301 - 1310   2017.6

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1039/C7PP00110J

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Books 1

  1. 研究者の結婚生活: 恋愛はぜんぜん科学的じゃない⁉ International journal

    高山善光( Role: Contributor ,  結婚式はウェディング姿で研究発表)

    日本の研究者出版  2020.10 

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    Language:Japanese Book type:General book, introductory book for general audience

Presentations 4

  1. 太古より受け継がれる深海サンゴの発光 Invited

    別所-上原 学

    新妻免疫塾 (YouTubeチャンネル)  2020.6.22 

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    Language:Japanese   Presentation type:Oral presentation (invited, special)  

  2. 生物発光の収斂進化 Invited International conference

    別所-上原 学

    永井研公開セミナー  2020.6.21 

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    Language:Japanese   Presentation type:Oral presentation (invited, special)  

  3. Kleptoprotein Bioluminescence Invited International conference

    Manabu Bessho-Uehara

    Yosakoi Ecology Seminar  2020.6.16  Noriyuki Suzuki

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    Language:Japanese   Presentation type:Oral presentation (invited, special)  

  4. Evolution of bioluminescence in the sea Invited International coauthorship International conference

    Manabu Bessho-Uehra

    Moss Landing Marine Laboratories Seminar  2020.2.20 

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    Language:English   Presentation type:Public lecture, seminar, tutorial, course, or other speech  

Other research activities 1

  1. Glow-in-the-dark corals light up the deep sea

    2020.7

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    A team of MBARI researchers filmed bioluminescence from a variety of deep octocorals (soft corals, sea pens, and gorgonians) in their own habitat for the first time. The scientists worked with the pilots of the remotely operated vehicle (ROV) Doc Ricketts to equip the vehicle with an extremely light-sensitive video camera. Since many animals only glow when disturbed, they filmed the animals while gently touching them with the ROV’s manipulator arm or a soft paint brush. They found that many of the most common deep-sea corals can create their own light, including several groups not previously known to glow. The researchers also analyzed the animals’ DNA to look for the genes that might be creating the luminescence.

KAKENHI (Grants-in-Aid for Scientific Research) 4

  1. Kleptoprotein bioluminescence in fish: Molecular mechanism of protein uptake and its evolutionary origin

    Grant number:21K15144  2021.4 - 2025.3

    Grants-in-Aid for Scientific Research  Grant-in-Aid for Early-Career Scientists

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    Authorship:Principal investigator  Grant type:Competitive

    Grant amount:\4810000 ( Direct Cost: \3700000 、 Indirect Cost:\1110000 )

  2. Taxonomical review of Pempheridae and the origin and evolution of bioluminescence

    Grant number:21K06313  2021.4 - 2025.3

    Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

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    Authorship:Coinvestigator(s)  Grant type:Competitive

  3. Molecular mechanism involved in the uptake of ostracod luciferase into luminous fish Parapriacanthus

    Grant number:20K22627  2020.9 - 2022.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Research Activity Start-up  Grant-in-Aid for Research Activity Start-up

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    Authorship:Principal investigator  Grant type:Competitive

    Grant amount:\2860000 ( Direct Cost: \2200000 、 Indirect Cost:\660000 )

  4. ウミホタルを食べて光る魚-キンメモドキの発光メカニズムの解明-

    Grant number:15J00296  2015.4 - 2017.3

    日本学術振興会  科学研究費助成事業 特別研究員奨励費  特別研究員奨励費

    別所 学

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    Authorship:Principal investigator  Grant type:Competitive

    Grant amount:\1900000 ( Direct Cost: \1900000 )

    キンメモドキから発光活性を指標にルシフェラーゼを精製し、単離した。RNAseqとMS/MSにより、タンパク質の全長配列を決定し、同定した。その後、リコンビナントルシフェラーゼを抗原とし、抗体を作成し、天然型のルシフェラーゼを結合したカラムを用いて、アフィニティー精製を行なった。その結果、質の高い抗ルシフェラーゼ抗体が得られた。また、キンメモドキの新たな供給経路を確立した。本年度は、これらの材料を元に、ウェスタンブロットと免疫組織化学により、キンメモドキにおける同定したルシフェラーゼの局在が発光器官にのみあることを確認できた。現在、組織の微細構造を確認中であり、並行して論文化を進めている。
    また、発光の進化を議論するために、同じくウミホタルルシフェリンを基質とする発光魚についてもサンプルを集めている。本年度は、スズキ目であるが異なる科のツマグロイシモチ、ガマアンコウ目のイサリビガマアンコウを入手できた。ツマグロイシモチは高知で採取され、また、イサリビガマアンコウはアメリカ合衆国のカリフォルニアで採取された。ツマグロイシモチの系統分類的位置付けは、まだはっきりしていないので、発光の進化を議論する上で、今後、分類学者との共同研究が必要になる。
    これらの材料に対して、現在、キンメモドキのルシフェラーゼと相同性があるかを調べるため、同様に、ウェスタンブロットと免疫組織化学による検出を進めている。今後、反応が出た場合、プルダウンからのMS/MS解析により、タンパク質の同定を進め、配列から、進化的な考察を深めたい。

 

Media Coverage 3

  1. How a fish steals its ability to glow Internet

    The New York Times  2020.1

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    Author:Other 

    Article about kleptoprotein bioluminescence (Bessho-Uehara et al., 2020, Science Advances)

  2. 深海発光サンゴ 新たに4種 Newspaper, magazine

    中日新聞社  中日新聞  2020.10

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    Author:Other 

  3. Fish Steals Bioluminescence from Prey Internet

    The Scientist  2020.1

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    Author:Other 

    An article about kleptoprotein bioluminescence