Updated on 2022/02/08

写真a

 
CAMAGNA Maurizio
 
Organization
Graduate School of Bioagricultural Sciences Designated assistant professor
Title
Designated assistant professor

Degree 4

  1. PhD ( 2020.3   Nagoya University ) 

  2. Master of Science ( 2016.10   University of Freiburg ) 

  3. Bachelor of Science ( 2014.10   University of Würzburg ) 

  4. Bachelor of Science ( 2007.10   University of Furtwangen ) 

 

Papers 13

  1. AP2/ERF Transcription Factor NbERF-IX-33 Is Involved in the Regulation of Phytoalexin Production for the Resistance of Nicotiana benthamiana to Phytophthora infestans

    Sayaka Imano, Mayuka Fushimi, Maurizio Camagna, Akiko Tsuyama-Koike, Hitoshi Mori, Akira Ashida, Aiko Tanaka, Ikuo Sato, Sotaro Chiba, Kazuhito Kawakita, Makoto Ojika, Daigo Takemoto

    Frontiers in Plant Science   Vol. 12   2022.1

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Frontiers Media {SA}  

    <jats:p>Plants recognize molecular patterns unique to a certain group of microbes to induce effective resistance mechanisms. Elicitins are secretory proteins produced by plant pathogenic oomycete genera including <jats:italic>Phytophthora</jats:italic> and <jats:italic>Pythium.</jats:italic> Treatment of INF1 (an elicitin produced by <jats:italic>P. infestans</jats:italic>) induces a series of defense responses in <jats:italic>Nicotiana</jats:italic> species, including reactive oxygen species (ROS) production, transient induction of ethylene production, hypersensitive cell death and accumulation of the sesquiterpenoid phytoalexin capsidiol. In this study, we analyzed the expression profiles of <jats:italic>N. benthamiana</jats:italic> genes after INF1 treatment by RNAseq analysis. Based on their expression patterns, <jats:italic>N. benthamiana</jats:italic> genes were categorized into 20 clusters and 4,761 (8.3%) out of 57,140 genes were assigned to the clusters for INF1-induced genes. All genes encoding enzymes dedicated to capsidiol production, 5-<jats:italic>epi</jats:italic>-aristolochene (EA) synthase (<jats:italic>NbEAS</jats:italic>, 10 copies) and EA dehydrogenase (<jats:italic>NbEAH</jats:italic>, 6 copies), and some genes for ethylene production, such as 1-aminocyclopropane 1-carboxylate (ACC) synthase (<jats:italic>NbACS</jats:italic>) and ACC oxidase (<jats:italic>NbACO</jats:italic>), were significantly upregulated by INF1 treatment. Analysis of <jats:italic>NbEAS1</jats:italic> and <jats:italic>NbEAS4</jats:italic> promoters revealed that AGACGCC (GCC box-like motif) is the essential cis-element required for INF1-induced expression of <jats:italic>NbEAS</jats:italic> genes. Given that the GCC box is known to be targeted by ERF (ethylene-responsive factor) transcription factors, we created a complete list of <jats:italic>N. benthamiana</jats:italic> genes encoding AP2/ERF family transcription factors, and identified 45 out of 337 <jats:italic>AP2/ERF</jats:italic> genes in the clusters for INF1-induced genes. Among INF1-induced <jats:italic>NbERF</jats:italic> genes, silencing of <jats:italic>NbERF-IX-33</jats:italic> compromised resistance against <jats:italic>P. infestans</jats:italic> and INF1-induced production of capsidiol. Recombinant NbERF-IX-33 protein can bind to the promoter sequence of <jats:italic>NbEAS4</jats:italic>, suggesting that NbERF-IX-33 is a transcription factor directly regulating the expression of genes for phytoalexin production.</jats:p>

    DOI: 10.3389/fpls.2021.821574

  2. Expression profiles of genes for enzymes involved in capsidiol production inNicotiana benthamiana

    Rin Soriya, Imano Sayaka, Camagna Maurizio, Suzuki Takamasa, Tanaka Aiko, Sato Ikuo, Chiba Sotaro, Kawakita Kazuhito, Takemoto Daigo

    JOURNAL OF GENERAL PLANT PATHOLOGY   Vol. 86 ( 5 ) page: 340 - 349   2020.9

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    Language:Japanese   Publishing type:Research paper (scientific journal)   Publisher:Journal of General Plant Pathology  

    In Solanaceae plants, the major phytoalexins produced during the induction of plant defense are sesquiterpenoids, such as capsidiol for Nicotiana species and rishitin for Solanum species, which are produced via the mevalonate (MVA) pathway. Eight enzymes are involved in the production of farnesyl pyrophosphate (FPP), the common precursor of phytosterols for maintaining membrane integrity and sesquiterpenoid phytoalexins for plant defense. In this study, expression profiles of N. benthamiana genes for the production of capsidiol during the induction of disease resistance were investigated. In the genome of N. benthamiana, multiple copies of genes for each enzyme in the MVA pathway were identified, and the expression of some, but not all MVA genes, was significantly upregulated after inoculation with Phytophthora infestans, or treatment with the INF1 elicitor, a secretory protein of P. infestans. For genes encoding enzymes involved in capsidiol production, 10 copies of 5-epi-aristolochene synthase (NbEAS) and six copies of 5-epi-aristolochene dihydroxylase (NbEAH) were identified, and all copies were significantly upregulated during the induction of disease resistance. Gene silencing of MAP kinase genes NbWIPK, NbSIPK, and NbNTF4 compromised INF1-induced production of phytoalexins. Expression analysis of control and NbWIPK/SIPK/NTF4-silenced plants indicated that most of the MVA genes are not under the control of these MAP kinases. In contrast, the expression pattern of NbWIPK/SIPK/NTF4 and all copies of NbEAH genes showed significant correlation, suggesting that MAP kinases are critical regulators of transcriptional upregulation of specific genes for capsidiol production.

    DOI: 10.1007/s10327-020-00931-5

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  3. In Vitro Assessment of Biocontrol Effects on Fusarium Head Blight and Deoxynivalenol (DON) Accumulation by DON-Degrading Bacteria Reviewed

    Hiroyuki Morimura, Michihiro Ito, Shigenobu Yoshida, Motoo Koitabashi, Seiya Tsushima, Maurizio Camagna, Sotaro Chiba, Daigo Takemoto, Kazuhito Kawakita, Ikuo Sato

    Toxins   Vol. 12 ( 6 ) page: 399 - 399   2020.6

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    Language:Japanese   Publishing type:Research paper (scientific journal)   Publisher:MDPI AG  

    Fusarium head blight (FHB) of cereals is a severe disease caused by the Fusarium graminearum species complex. It leads to the accumulation of the mycotoxin deoxynivalenol (DON) in grains and other plant tissues and causes substantial economic losses throughout the world. DON is one of the most troublesome mycotoxins because it is a virulence factor to host plants, including wheat, and exhibits toxicity to plants and animals. To control both FHB and DON accumulation, a biological control approach using DON-degrading bacteria (DDBs) is promising. Here, we performed a disease control assay using an in vitro petri dish test composed of germinated wheat seeds inoculated with F. graminearum (Fg) and DDBs. Determination of both grown leaf lengths and hyphal lesion lengths as a measure of disease severity showed that the inoculation of seeds with the DDBs Devosia sp. strain NKJ1 and Nocardioides spp. strains SS3 or SS4 were protective against the leaf growth inhibition caused by Fg. Furthermore, it was as effective against DON accumulation. The inoculation with strains SS3 or SS4 also reduced the inhibitory effect on leaves treated with 10 µg mL−1 DON solution (without Fg). These results indicate that the DDBs partially suppress the disease by degrading DON.

    DOI: 10.3390/toxins12060399

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  4. Detoxification of the solanaceous phytoalexins rishitin, lubimin, oxylubimin and solavetivone via a cytochrome P450 oxygenase

    Camagna Maurizio, Ojika Makoto, Takemoto Daigo

    PLANT SIGNALING & BEHAVIOR   Vol. 15 ( 2 ) page: 1707348   2020.2

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1080/15592324.2019.1707348

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  5. Expression, Purification, and Enzyme Activity Assay of Phytoene Synthase In Vitro.

    Camagna M, Welsch R

    Methods in molecular biology (Clifton, N.J.)   Vol. 2083   page: 39-52   2020

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1007/978-1-4939-9952-1_3

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  6. Nicotiana benthamiana exportin 1 is required for elicitor-induced phytoalexin production, cell death induction, and resistance against potato late blight pathogen Phytophthora infestans

    Mizuno Yuri, Imano Sayaka, Camagna Maurizio, Suzuki Takamasa, Tanaka Aiko, Sato Ikuo, Chiba Sotaro, Kawakita Kazuhito, Takemoto Daigo

    JOURNAL OF GENERAL PLANT PATHOLOGY   Vol. 85 ( 5 ) page: 347-355   2019.9

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1007/s10327-019-00855-9

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  7. Nicotiana benthamiana RanBP1-1 Is Involved in the Induction of Disease Resistance via Regulation of Nuclear-Cytoplasmic Transport of Small GTPase Ran

    Mizuno Yuri, Ohtsu Mina, Shibata Yusuke, Tanaka Aiko, Camagna Maurizio, Ojika Makoto, Mori Hitoshi, Sato Ikuo, Chiba Sotaro, Kawakita Kazuhito, Takemoto Daigo

    FRONTIERS IN PLANT SCIENCE   Vol. 10   page: 222   2019.3

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.3389/fpls.2019.00222

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  8. Enzyme Fusion Removes Competition for Geranylgeranyl Diphosphate in Carotenogenesis

    Camagna Maurizio, Grundmann Alexander, Bar Cornelia, Koschmieder Julian, Beyer Peter, Welsch Ralf

    PLANT PHYSIOLOGY   Vol. 179 ( 3 ) page: 1013-1027   2019.3

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1104/pp.18.01026

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  9. Multiplexed antibody detection from blood sera by immobilization of in vitro expressed antigens and label-free readout via imaging reflectometric interferometry (iRIf)

    Bender Julian, Bognar Sabine, Camagna Maurizio, Donauer Julia A. M., Eble Julian W., Emig Ramona, Fischer Sabrina, Jesser Rabea, Keilholz Luisa, Kokotek Daniel M. U., Neumann Julika, Nicklaus Simon, Weernink Ricardo R. Q. P. T. Oude, Stuehn Lara G., Woessner Nathalie, Kraemer Stefan D., Schwenk Philipp, Gensch Nicole, Roth Guenter, Ulbrich Maximilian H.

    BIOSENSORS & BIOELECTRONICS   Vol. 115   page: 97-103   2018.9

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.bios.2018.05.022

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  10. Resistance to Phytophthora infestans: exploring genes required for disease resistance in Solanaceae plants

    Takemoto Daigo, Shibata Yusuke, Ojika Makoto, Mizuno Yuri, Imano Sayaka, Ohtsu Mina, Sato Ikuo, Chiba Sotaro, Kawakita Kazuhito, Rin Soriya, Camagna Maurizio

    JOURNAL OF GENERAL PLANT PATHOLOGY   Vol. 84 ( 5 ) page: 312-320   2018.9

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1007/s10327-018-0801-8

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  11. Measuring Secretion of Capsidiol in Leaf Tissues of Nicotiana benthamiana

    Kuroyanagi Teruhiko, Camagna Maurizio, Takemoto Daigo

    BIO-PROTOCOL   Vol. 8 ( 15 )   2018.8

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.21769/BioProtoc.2954

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  12. Hypersensitive Response in Plants Reviewed

    Maurizio Camagna, Daigo Takemoto

    eLS     2018.1

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1002/9780470015902.a0020103.pub2

  13. Strigolactone Levels in Dicot Roots Are Determined by an Ancestral Symbiosis-Regulated Clade of the PHYTOENE SYNTHASE Gene Family.

    Stauder R, Welsch R, Camagna M, Kohlen W, Balcke GU, Tissier A, Walter MH

    Frontiers in plant science   Vol. 9   page: 255   2018

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.3389/fpls.2018.00255

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MISC 2

  1. ベンサミアナのジャガイモ疫病菌抵抗性における核膜孔を介した物質輸送に関与するExportinの機能解析

    水野邑里, 今野沙弥香, CAMAGNA Maurizio, 森仁志, 鈴木孝征, 田中愛子, 佐藤育男, 千葉壮太郎, 川北一人, 竹本大吾

    日本植物病理学会大会プログラム・講演要旨予稿集   Vol. 2019   page: 154   2019.3

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    Language:Japanese  

    J-GLOBAL

  2. ベンサミアナの核膜孔を介した物質輸送に関与するExportinのジャガイモ疫病菌抵抗性における機能解析

    水野邑里, 今野沙弥香, CAMAGNA Maurizio, 鈴木孝征, 田中愛子, 佐藤育男, 千葉壮太郎, 川北一人, 竹本大吾

    日本植物生理学会年会(Web)   Vol. 60th   page: 866 (WEB ONLY)   2019

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    Language:Japanese  

    J-GLOBAL