Updated on 2022/04/12

写真a

 
CHOKRADJAROEN Chayanaphat
 
Organization
Graduate School of Engineering Assistant Professor
Graduate School
Graduate School of Engineering
Undergraduate School
School of Engineering Materials Science and Engineering
Title
Assistant Professor
Contact information
メールアドレス
Profile
Research guidance on biological compounds such as chitosan and sustainable materials using solution plasma.
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Degree 3

  1. DOCTOR OF PHILOSOPHY ( 2018.6   Chulalongkorn University ) 

  2. MASTER OF ENGINEERING (MATERIAL ENGINEERING) ( 2011.3   Kanazawa University ) 

  3. BACHELOR OF ENGINEERING (BIOCHEMICAL ENGINEERING) ( 2008.7   Thammasat University ) 

Research Interests 3

  1. Biopolymer

  2. Plasma

  3. Modification

Research Areas 1

  1. Nanotechnology/Materials / Polymer chemistry  / Biopolymer

Current Research Project and SDGs 1

  1. Modification of chitosan by solution plasma process and its biomedical and energy application

 

Papers 10

  1. Insight on Solution Plasma in Aqueous Solution and Their Application in Modification of Chitin and Chitosan.

    Chokradjaroen C, Niu J, Panomsuwan G, Saito N

    International journal of molecular sciences   Vol. 22 ( 9 )   2021.4

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    Language:English   Publisher:International Journal of Molecular Sciences  

    Sustainability and environmental concerns have persuaded researchers to explore renewable materials, such as nature-derived polysaccharides, and add value by changing chemical structures with the aim to possess specific properties, like biological properties. Meanwhile, finding methods and strategies that can lower hazardous chemicals, simplify production steps, reduce time consumption, and acquire high-purified products is an important task that requires attention. To break through these issues, electrical discharging in aqueous solutions at atmospheric pressure and room temperature, referred to as the “solution plasma process”, has been introduced as a novel process for modification of nature-derived polysaccharides like chitin and chitosan. This review reveals insight into the electrical discharge in aqueous solutions and scientific progress on their application in a modification of chitin and chitosan, including degradation and deacetylation. The influencing parameters in the plasma process are intensively explained in order to provide a guideline for the modification of not only chitin and chitosan but also other nature-derived polysaccharides, aiming to address economic aspects and environmental concerns.

    DOI: 10.3390/ijms22094308

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  2. Simultaneous synthesis of graphite-like and amorphous carbon materials via solution plasma and their evaluation as additive materials for cathode in Li-O<sub>2</sub> battery.

    Chokradjaroen C, Watanabe H, Ishii T, Ishizaki T

    Scientific reports   Vol. 11 ( 1 ) page: 6261   2021.3

  3. N-Doped few-layer graphene encapsulated Pt-based bimetallic nanoparticles: Via solution plasma as an efficient oxygen catalyst for the oxygen reduction reaction

    Phan P.Q., Naraprawatphong R., Pornaroontham P., Park J., Chokradjaroen C., Saito N.

    Materials Advances   Vol. 2 ( 1 ) page: 322 - 335   2021

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    Language:Japanese   Publisher:Materials Advances  

    N-Doped few-layer graphene encapsulated Pt-based bimetallic nanoparticles were successfully synthesized via the solution plasma (SP) method. This synthesis strategy can be achieved in one pot by using only pure dimethylformamide (DMF) as the reaction solution at room temperature and atmospheric pressure. The structural analyses exhibited a fine core-shell structured nanoparticle (2-4 nm), which had Pt and Pt-based bimetallic nanoparticles as a core and N-doped few-layer graphene (NFG), having 2-4 layers, as a shell. Pt-based bimetallic cores (i.e., PtAu, PtAg, and PtPd) were varied depending on the metal electrodes used in the SP process. The electrocatalytic activity toward the oxygen reduction reaction (ORR) of the obtained samples in an acidic solution was found to be acceptable, while possessing excellent durability, compared to those of the commercial carbon-supported Pt catalyst. The effective ORR activity was possibly attributed to the synergistic effect of the Pt-based bimetallic core and N-doped graphene shell which contained high amounts of pyridinic-N and quaternary-N. The results in a single fuel cell test expressed the superiority of our SP-derived sample in the fuel cell performances to commercial Pt/C. This study not only proposed potential alternative ORR catalysts with acceptable electrocatalytic activity and high durability but also provided guidelines for the facile synthesis of carbon-based core-shell nanostructured materials with low chemical use via the SP process. This journal is

    DOI: 10.1039/d0ma00718h

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  4. Effect of electrical discharge plasma on cytotoxicity against cancer cells of N,O-carboxymethyl chitosan-stabilized gold nanoparticles.

    Chokradjaroen C, Rujiravanit R, Theeramunkong S, Saito N

    Carbohydrate polymers   Vol. 237   page: 116162   2020.6

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    Language:English   Publisher:Carbohydrate Polymers  

    Electrical discharge plasma in a liquid phase can generate reactive species, e.g. hydroxyl radical, leading to rapid reactions including degradation of biopolymers. In this study, the effect of plasma treatment time on physical properties and cytotoxicity against cancer cells of N,O-carboxymethyl chitosan-stabilized gold nanoparticles (CMC-AuNPs) was investigated. AuNPs were synthesized by chemical reduction of HAuCl4 in 2 % CMC solution to obtain CMC-AuNPs, before being subjected to the plasma treatment. Results showed that the plasma treatment not only led to the reduction of hydrodynamic diameters of CMC-AuNPs from 400 nm to less than 100 nm by the plasma-induced degradation of CMC but also provided the narrow size distribution of AuNPs having diameters in the range of 2–50 nm, that were existing in CMC-AuNPs. In addition, the plasma-treated CMC-AuNPs could significantly reduce the percentage of cell viability of breast cancer cells by approximately 80 % compared to the original CMC and CMC-AuNPs.

    DOI: 10.1016/j.carbpol.2020.116162

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  5. Simultaneous deacetylation and degradation of chitin hydrogel by electrical discharge plasma using low sodium hydroxide concentrations.

    Rujiravanit R, Kantakanun M, Chokradjaroen C, Vanichvattanadecha C, Saito N

    Carbohydrate polymers   Vol. 228   page: 115377   2020.1

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    Language:English   Publisher:Carbohydrate Polymers  

    Electrical discharge plasma occurring in a liquid phase, so called solution plasma, can generate highly active species, e.g. free radicals, which can involve in various chemical reactions, leading to less chemical uses. In this study, solution plasma was applied to deacetylation of chitin aiming to reduce the use of alkali. It was found that solution plasma could induce deacetylation of chitin hydrogels that were dispersed in MeOH/water solutions containing low NaOH concentrations (1–12%). Due to the action of free radicals, some extent of chain session of the polymer occurred during the plasma treatment. The degree of deacetylation and molecular weight of the obtained chitosan were 78% and 220 kDa, respectively, after the plasma treatment for five cycles (1 h/cycle) by using 90% MeOH/water solution containing 12% NaOH. The obtained chitosan could completely dissolve in 2% acetic acid solution and had antibacterial activities against S. aureus and E. coli.

    DOI: 10.1016/j.carbpol.2019.115377

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  6. Synthesis of Au Nanoparticles in Natural Matrices by Liquid-Phase Plasma: Effects on Cytotoxic Activity against Normal and Cancer Cell Lines

    Treesukkasem N., Chokradjaroen C., Theeramunkong S., Saito N., Watthanaphanit A.

    ACS Applied Nano Materials   Vol. 2 ( 12 ) page: 8051 - 8062   2019.12

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    Language:Japanese   Publisher:ACS Applied Nano Materials  

    Wrapping of gold nanoparticles (AuNPs) with a biocompatible matrix, to gain AuNP colloids, is a general strategy to synthesize the AuNPs for biomedical purposes. This work reports the synthesis of AuNP colloids in the aqueous solution of several natural matrices using a liquid-phase plasma process. Two classes of natural substances used are sugars (including: glucose, fructose, and sucrose) and biopolymers (including: carboxymethyl cellulose, sodium alginate, and gelatin). All are negatively charged water-soluble substances that are claimed to be sources of energy for cells to grow and have a high potential to be compatible with them. The study has emerged since one question arises: "Do these matrices also promote the growth of cancer cells?" The synthesis is performed by generating plasma across a pair of electrodes immersed in an aqueous solution of a natural matrix containing a gold precursor (HAuCl4·3H2O). Two concentrations of the matrices (0.5 and 1.0% w/v) are used, and the plasma treatment times are varied (0, 10, and 30 min). The effect of the type and concentration of natural matrices as well as the plasma treatment time on the formation of AuNPs, along with their physical and chemical properties, including morphology, size, hydrodynamic diameter (dh), colloidal stability, and charge on the AuNP surface, is evaluated. We find that the charge of the AuNP surfaces could be altered by the plasma treatment. Eventually, cytotoxicity test results against normal (MRC-5) and cancer (H460 and HeLa) cell lines could not only answer our opening question but also suggest a rather complex response. Our findings indicate the great potential of the obtained AuNP colloids as a part of cancer therapy.

    DOI: 10.1021/acsanm.9b02106

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  7. Cytotoxicity against cancer cells of chitosan oligosaccharides prepared from chitosan powder degraded by electrical discharge plasma.

    Chokradjaroen C, Theeramunkong S, Yui H, Saito N, Rujiravanit R

    Carbohydrate polymers   Vol. 201   page: 20 - 30   2018.12

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    Language:English   Publisher:Carbohydrate Polymers  

    Chitosan oligosaccharides, which obtain from degradation of chitosan, possess some interesting molecular weight-dependent biological properties, especially anticancer activity. Therefore, the conversion of chitosan to chitosan oligosaccharides with specific molecular weight has been continuously investigated in order to find effective strategies that can achieve both economic feasibility and environmental concerns. In this study, a novel process was developed to heterogeneously degrade chitosan powder by highly active species generated by electrical discharge plasma in a dilute salt solution (0.02 M) without the addition of other chemicals. The degradation rate obtained from the proposed process was comparable to that obtained from some other methods with the addition of acids and oxidizing agents. Separation of the water-soluble degraded products containing chitosan oligosaccharides from the reaction solution was simply done by filtration. The obtained chitosan oligosaccharides were further evaluated for an influence of their molecular weights on cytotoxicity against cancer cells and the selectivity toward cancer and normal cells.

    DOI: 10.1016/j.carbpol.2018.08.037

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  8. Degradation of chitosan hydrogel dispersed in dilute carboxylic acids by solution plasma and evaluation of anticancer activity of degraded products

    Chokradjaroen C., Rujiravanit R., Theeramunkong S., Saito N.

    Japanese Journal of Applied Physics   Vol. 57 ( 1 )   2018.1

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    Language:Japanese   Publisher:Japanese Journal of Applied Physics  

    Chitosan is a polysaccharide that has been extensively studied in the field of biomedicine, especially its water-soluble degraded products called chitooligosaccharides (COS). In this study, COS were produced by the degradation of chitosan hydrogel dispersed in a dilute solution (i.e., 1.55 mM) of various kinds of carboxylic acids using a non-thermal plasma technology called solution plasma (SP). The degradation rates of chitosan were influenced by the type of carboxylic acids, depending on the interaction between chitosan and each carboxylic acid. After SP treatment, the water-soluble degraded products containing COS could be easily separated from the water-insoluble residue of chitosan hydrogel by centrifugation. The production yields of the COS were mostly higher than 55%. Furthermore, the obtained COS products were evaluated for their inhibitory effect as well as their selectivity against human lung cancer cells (H460) and human lung normal cells (MRC-5).

    DOI: 10.7567/JJAP.57.0102B5

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  9. In vitro cytotoxicity of carbon black nanoparticles synthesized from solution plasma on human lung fibroblast cells

    Panomsuwan G., Chokradjaroen C., Rujiravanit R., Ueno T., Saito N.

    Japanese Journal of Applied Physics   Vol. 57 ( 1 )   2018.1

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    Language:Japanese   Publisher:Japanese Journal of Applied Physics  

    Carbon black nanoparticles (CB-NPs) have been synthesized from liquid benzene by a solution plasma method at room temperature and atmospheric pressure. The morphological observation by scanning electron microscopy revealed the agglomeration of aggregated fine particles. The synthesized CB-NPs were predominantly amorphous as confirmed by X-ray diffraction. The in vitro cytotoxicity of CB-NPs on the human lung fibroblast (MRC-5) cell line was assessed by the 3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and systematically compared with those of two types of commercial carbon blacks (i.e., Vulcan XC-72 and Ketjenblack EC-600JD). Cell viabilities were studied at different concentrations of 32.5, 65, 125, and 250 μg/mL. It was found that the CB-NPs derived from solution plasma exhibited a lower cytotoxicity on the MRC-5 cells than the other two comparative carbon blacks. The viability of MRC-5 cells exposed to CB-NPs remained higher than 90% even at a high concentration of 250 μg/mL. This result preliminarily confirmed the biosafety and potential use of CB-NPs in the field of biological applications.

    DOI: 10.7567/JJAP.57.0102BG

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  10. Enhanced degradation of chitosan by applying plasma treatment in combination with oxidizing agents for potential use as an anticancer agent.

    Chokradjaroen C, Rujiravanit R, Watthanaphanit A, Theeramunkong S, Saito N, Yamashita K, Arakawa R

    Carbohydrate polymers   Vol. 167   page: 1 - 11   2017.7

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    Language:English   Publisher:Carbohydrate Polymers  

    Solution plasma (SP) treatment in combination with oxidizing agents, i.e., hydrogen peroxide (H2O2), potassium persulfate (K2S2O8) and sodium nitrite (NaNO2) were adopted to chitosan degradation in order to achieve fast degradation rate, low chemicals used and high yield of low-molecular-weight chitosan and chitooligosaccharide (COS). Among the studied oxidizing agents, H2O2 was found to be the best choice in terms of appreciable molecular weight reduction without major change in chemical structure of the degraded products of chitosan. By the combination with SP treatment, dilute solution of H2O2 (4–60 mM) was required for effective degradation of chitosan. The combination of SP treatment and dilute solution of H2O2 (60 mM) resulted in the great reduction of molecular weight of chitosan and water-soluble chitosan was obtained as a major product. The resulting water-soluble chitosan was precipitated to obtain COS. An inhibitory effect against cervical cancer cell line (HeLa cells) of COS was also examined.

    DOI: 10.1016/j.carbpol.2017.03.006

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Presentations 10

  1. Green synthesis of chitosan oligosaccharide/silver nanocomposites and their enhancement of selectivity against cancer cells. International conference

    C. Chokradjaroen, R. Rujiravanit, S. Theeramunkong, N. Saito

    2nd International Conference on Composite Material, Polymer Science and Engineering  2018 

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    Event date: 2018

    Language:English   Presentation type:Oral presentation (general)  

    Venue:Osaka  

  2. Preparation of chitooligosaccharides loaded with metal nanoparticles via solution plasma sputtering and evaluation of their anticancer activity International conference

    C. Chokradjaroen, R. Rujiravanit, S. Theeramunkong, N. Saito

    Workshop on Biomimetic and Energy-saving Materials  2017 

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    Event date: 2017

    Language:English   Presentation type:Poster presentation  

    Venue:Shanghai, China  

  3. Degradation of gold nanoparticle-loaded carboxymethyl chitosan via non-thermal plasma and evaluation of their anticancer activity

    C. Chokradjaroen, R. Rujiravanit, S. Theeramunkong, N. Saito

    The 5th International Workshop & the 4th International Mini Workshop on Solution Plasma and Molecular Technologies (SPM-5)  2017 

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    Event date: 2017

    Language:English   Presentation type:Oral presentation (general)  

    Venue:Greifswald, Germany  

  4. Degradation of gold nanoparticle-loaded carboxymethyl chitosan by applying solution plasma treatment and their anticancer activity Invited

    C. Chokradjaroen, R. Rujiravanit, S. Theeramunkong, N. Saito

    RGJ-Ph.D. Congress 18  2017 

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    Event date: 2017

    Language:English   Presentation type:Oral presentation (general)  

    Venue:Bangkok, Thailand  

  5. Effect of chemical structures of organic acids on degradation of chitosan hydrogel via solution plasma treatment

    C. Chokradjaroen, R. Rujiravanit, S. Theeramunkong, N. Saito

    The 7th Research Symposium on Petrochemical and Materials Technology and the 22nd PPC Symposium on Petroleum, Petrochemicals, and Polymers  2016 

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    Event date: 2016

    Language:English   Presentation type:Oral presentation (general)  

  6. Degradation of gold nanoparticle (AuNP)-loaded chitosan hydrogel by applying solution plasma treatment International conference

    C. Chokradjaroen, R. Rujiravanit, A. Watthanaphanit, N. Saito

    11th International Symposium in Science and Technology at Kansai University   2016 

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    Event date: 2016

    Language:English   Presentation type:Oral presentation (general)  

    Venue:Osaka  

  7. Utilization of oxidizing agents in combination with solution plasma treatment for degradation of chitosan

    C. Chokradjaroen, R. Rujiravanit, A. Watthanaphanit, N. Saito

    Pure and Applied chemistry International Conference 2016  2016 

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    Event date: 2016

    Language:English   Presentation type:Oral presentation (general)  

    Venue:Bangkok, Thailand  

  8. Hydrolysis of chitosan via solution plasma process in the presence of dilute organic acids

    C. Chokradjaroen, N. Saito, R. Rujiravanit

    The 12th International Conference of the European Chitin Society  2015 

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    Event date: 2015

    Language:English   Presentation type:Poster presentation  

    Venue:Munster, Germany  

  9. Chitosan depolymerization by combination of sodium salts and solution plasma process International conference

    C. Chokradjaroen, N. Saito, R. Rujiravanit

    The 3rd International Workshop on Solution Plasma and Molecular Technologies (SPM-3)  2015 

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    Event date: 2015

    Language:English   Presentation type:Oral presentation (general)  

    Venue:Bangkok, Thailand  

  10. Effect of solvents and polymer concentrations on the chitosan depolymerization via solution plasma process (SPP) International conference

    C. Chokradjaroen, N. Saito, R. Rujiravanit

    The 2014 IUPAC World Polymer Congress (MACRO 2014)  2014 

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    Event date: 2014

    Venue:Chiangmai, Thailand  

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