2021/11/30 更新

写真a

ナカムラ カエ
中村 香江
NAKAMURA Kae
所属
低温プラズマ科学研究センター バイオシステム科学部門 特任講師
職名
特任講師

学位 1

  1. 博士(医学) ( 2009年3月   名古屋大学 ) 

 

論文 26

  1. Preclinical Verification of the Efficacy and Safety of Aqueous Plasma for Ovarian Cancer Therapy 査読有り

    Nakamura Kae, Yoshikawa Nobuhisa, Mizuno Yuko, Ito Miwa, Tanaka Hiromasa, Mizuno Masaaki, Toyokuni Shinya, Hori Masaru, Kikkawa Fumitaka, Kajiyama Hiroaki

    CANCERS   13 巻 ( 5 ) 頁: 1 - 15   2021年3月

     詳細を見る

    担当区分:筆頭著者, 責任著者   記述言語:日本語   出版者・発行元:Cancers  

    Epithelial ovarian cancer (EOC) is the most lethal gynecologic malignancy. The major cause of EOC’s lethality is that intraperitoneal recurrence occurs with high frequency due to occult metastasis. We had demonstrated that plasma-activated medium (PAM) exerts a metastasis-inhibitory effect on ovarian cancer in vitro and in vivo. Here we investigated how PAM inhibits intraperitoneal metastasis. We studied PAM’s inhibition of micro-dissemination onto the omentum by performing in vivo imaging in combination with a sequential histological analysis. The results revealed that PAM induced macrophage infiltration into the disseminated lesion. The iNOS-positive signal was co-localized at the macrophages in the existing lesion, indicating that PAM might induce M1-type macrophages. This may be another mechanism of the antitumor effect through a PAM-evoked immune response. Intraperitoneal lavage with plasma-activated lactate Ringer’s solution (PAL) significantly improved the overall survival rate in an ovarian cancer mouse model. Our results demonstrated the efficiency and practicality of aqueous plasma for clinical applications.

    DOI: 10.3390/cancers13051141

    Web of Science

    Scopus

    PubMed

  2. Adjusted multiple gases in the plasma flow induce differential antitumor potentials of plasma-activated solutions 招待有り 査読有り

    Nakamura Kae, Yoshikawa Nobuhisa, Yoshihara Masato, Ikeda Yoshiki, Higashida Akihiro, Niwa Akihiro, Jindo Takahiro, Tanaka Hiromasa, Ishikawa Kenji, Mizuno Masaaki, Toyokuni Shinya, Hori Masaru, Kikkawa Fumitaka, Kajiyama Hiroaki

    PLASMA PROCESSES AND POLYMERS   17 巻 ( 10 )   2020年10月

     詳細を見る

    担当区分:筆頭著者   記述言語:日本語   出版者・発行元:Plasma Processes and Polymers  

    We present a novel plasma system that can generate a plasma-activated medium by changing the proportion of reactive gases such as oxygen, nitrogen, and hydrogen in the plasma flow. The correlation between the biological activity of plasma-activated solutions prepared under multiple plasma gas-flow conditions was evaluated. Mixed nitrogen, in addition to oxygen, in the gas flow is most powerful for producing plasma-activated Ringer's lactate solution against antitumor effects on ovarian cancer cells as compared with oxygen or nitrogen alone. The antitumor effect of plasma-activated solutions is controllable by the modification of the proportion of reactive gases (especially nitrogen and oxygen gases) in the plasma flow. These results suggest that the plasma flow conditions may be one of the candidates for the specifications of the plasma-activated solutions to the therapeutic effect.

    DOI: 10.1002/ppap.201900259

    Web of Science

    Scopus

  3. Novel Intraperitoneal Treatment With Non-Thermal Plasma-Activated Medium Inhibits Metastatic Potential of Ovarian Cancer Cells 査読有り

    Nakamura Kae, Peng Yang, Utsumi Fumi, Tanaka Hiromasa, Mizuno Masaaki, Toyokuni Shinya, Hori Masaru, Kikkawa Fumitaka, Kajiyama Hiroaki

    SCIENTIFIC REPORTS   7 巻 ( 1 ) 頁: 6085   2017年7月

     詳細を見る

  4. Pro-tumoral behavior of omental adipocyte-derived fibroblasts in tumor microenvironment at the metastatic site of ovarian cancer 査読有り

    Iyoshi Shohei, Yoshihara Masato, Nakamura Kae, Sugiyama Mai, Koya Yoshihiro, Kitami Kazuhisa, Uno Kaname, Mogi Kazumasa, Tano Sho, Tomita Hiroyuki, Kajiwara Keiji, Taki Masayasu, Yamaguchi Shigehiro, Nawa Akihiro, Kajiyama Hiroaki

    INTERNATIONAL JOURNAL OF CANCER   149 巻 ( 11 ) 頁: 1961 - 1972   2021年12月

     詳細を見る

    記述言語:日本語   出版者・発行元:International Journal of Cancer  

    Adipocyte-rich omentum offers “good soil” for disseminating ovarian cancer (OvCa), contributing to therapeutic difficulty. However, little is understood about the association between adipocytes and tumor growth at peritoneal dissemination site. Herein, we report the induction of adipocyte dedifferentiation by OvCa cells and pro-tumorigenic effects of resulted adipocyte-derived fibroblasts. We confirmed that malignant ascites promoted the dedifferentiation of the primary human adipocytes obtained from surgical omental specimen into omental adipocyte-derived fibroblast (O-ADF) that possess both mesenchymal stem cell and myofibroblast-like features. This promotion of dedifferentiation by malignant ascites was blocked by addition of Wnt signaling inhibitor. The effects of dedifferentiated adipocytes in proliferation and migration of OvCa cells were analyzed with in vitro coculturing experimental models and in vivo mice model, and we demonstrated that OvCa cell lines showed enhanced proliferative characteristics, as well as increased migratory abilities upon coculturing with O-ADF. Additionally, exogenous transforming growth factor-β1 augmented desmoplastic morphological change of O-ADF, leading to higher proliferative ability. Our results suggest that OvCa cells promote dedifferentiation of peritoneal adipocytes by activating Wnt/β-catenin signaling, and generated O-ADFs exhibit pro-tumoral hallmarks.

    DOI: 10.1002/ijc.33770

    Web of Science

    Scopus

    PubMed

  5. Plasma-activated Ringer's lactate solution inhibits the cellular respiratory system in HeLa cells 査読有り

    Tanaka Hiromasa, Maeda Shogo, Nakamura Kae, Hashizume Hiroshi, Ishikawa Kenji, Ito Mikako, Ohno Kinji, Mizuno Masaaki, Motooka Yashiro, Okazaki Yasumasa, Toyokuni Shinya, Kajiyama Hiroaki, Kikkawa Fumitaka, Hori Masaru

    PLASMA PROCESSES AND POLYMERS   18 巻 ( 10 )   2021年10月

     詳細を見る

    記述言語:日本語   出版者・発行元:Plasma Processes and Polymers  

    Nonequilibrium atmospheric pressure plasma has enabled a variety of new applications in medicine, agriculture, and other industries. It is particularly noteworthy that plasma itself and/or plasma-activated culture medium have been shown to preferentially kill various cancer cells. We have previously developed a plasma-activated Ringer's lactate solution (PAL) for use as a new cancer treatment. In this study, behaviors of extracellular and intracellular reactive oxygen and nitrogen species in the cellular respiratory system of PAL-treated HeLa cells were investigated using an extracellular flux analyzer and a probe to measure mitochondrial membrane potential. In PAL-treated HeLa cells, extracellular hydrogen peroxide in PAL was found to be responsible for the induction of intracellular hydrogen peroxide and apoptosis, while other components in PAL are responsible for the induction of non-H2O2 intracellular ROS and non-apoptotic cell death, which should be clarified by further experiments. We believe that these are long-lived species derived from plasma-activated lactates. Furthermore, we found that the plasma-activated lactates inhibited glycolysis and the tricarboxylic acid (TCA) cycle, but not the electron transport chain in HeLa cells. These results suggest that PAL induces multiple modes of cell death, including apoptosis through hydrogen peroxide, and non-apoptotic cell death associated with the impairment of mitochondrial functions (glycolysis and TCA cycle). These findings shed light on the novel mechanism underlying plasma-activated lactate-induced cell death.

    DOI: 10.1002/ppap.202100056

    Web of Science

    Scopus

  6. Low temperature plasma irradiation products of sodium lactate solution that induce cell death on U251SP glioblastoma cells were identified 査読有り

    Tanaka Hiromasa, Hosoi Yugo, Ishikawa Kenji, Yoshitake Jun, Shibata Takahiro, Uchida Koji, Hashizume Hiroshi, Mizuno Masaaki, Okazaki Yasumasa, Toyokuni Shinya, Nakamura Kae, Kajiyama Hiroaki, Kikkawa Fumitaka, Hori Masaru

    SCIENTIFIC REPORTS   11 巻 ( 1 ) 頁: 18488   2021年9月

     詳細を見る

    記述言語:日本語   出版者・発行元:Scientific Reports  

    Low-temperature plasma is being widely used in the various fields of life science, such as medicine and agriculture. Plasma-activated solutions have been proposed as potential cancer therapeutic reagents. We previously reported that plasma-activated Ringer’s lactate solution exhibited selective cancer-killing effects, and that the plasma-treated L-sodium lactate in the solution was an anti-tumor factor; however, the components that are generated through the interactions between plasma and L-sodium lactate and the components responsible for the selective killing of cancer cells remain unidentified. In this study, we quantified several major chemical products, such as pyruvate, formate, and acetate, in plasma-activated L-sodium lactate solution by nuclear magnetic resonance analysis. We further identified novel chemical products, such as glyoxylate and 2,3-dimethyltartrate, in the solution by direct infusion-electrospray ionization with tandem mass spectrometry analysis. We found that 2,3-dimethyltartrate exhibited cytotoxic effects in glioblastoma cells, but not in normal astrocytes. These findings shed light on the identities of the components that are responsible for the selective cytotoxic effect of plasma-activated solutions on cancer cells, and provide useful data for the potential development of cancer treatments using plasma-activated L-sodium lactate solution.

    DOI: 10.1038/s41598-021-98020-w

    Web of Science

    Scopus

    PubMed

  7. Lysosomal nitric oxide determines transition from autophagy to ferroptosis after exposure to plasma-activated Ringer's lactate 査読有り

    Jiang Li, Zheng Hao, Lyu Qinying, Hayashi Shotaro, Sato Kotaro, Sekido Yoshitaka, Nakamura Kae, Tanaka Hiromasa, Ishikawa Kenji, Kajiyama Hiroaki, Mizuno Masaaki, Hori Masaru, Toyokuni Shinya

    REDOX BIOLOGY   43 巻   頁: 101989   2021年7月

     詳細を見る

    記述言語:日本語   出版者・発行元:Redox Biology  

    Non-thermal plasma (NTP), an engineered technology to generate reactive species, induces ferroptosis and/or apoptosis specifically in various-type cancer cells. NTP-activated Ringer's lactate (PAL) is another modality for cancer therapy at preclinical stage. Here we found that PAL induces selective ferroptosis of malignant mesothelioma (MM) cells, where non-targeted metabolome screening identified upregulated citrulline-nitric oxide (.NO) cycle as a PAL target .NO probe detected biphasic peaks transiently at PAL exposure with time-dependent increase, which was responsible for inducible .NO synthase (iNOS) overexpression through NF-κB activation. .NO and lipid peroxidation occupied lysosomes as a major compartment with increased TFEB expression. Not only ferrostatin-1 but inhibitors for .NO and/or iNOS could suppress this ferroptosis. PAL-induced ferroptosis accompanied autophagic process in the early phase, as demonstrated by an increase in essential amino acids, LC3B-II, p62 and LAMP1, transforming into the later phase with boosted lipid peroxidation. Therefore, .NO-mediated lysosomal impairment is central in PAL-induced ferroptosis.

    DOI: 10.1016/j.redox.2021.101989

    Web of Science

    Scopus

    PubMed

  8. Deficiency of cysteinyl cathepsin K suppresses the development of experimental intimal hyperplasia in response to chronic stress 査読有り

    Meng Xiangkun, Piao Limei, Wang Hailong, Inoue Aiko, Huang Zhe, Jiang Haiying, Nakamura Kae, Sasaki Takeshi, Li Xiang, Xu Wenhu, Yu Chenglin, Hu Lina, Wu Hongxian, Murohara Toyoaki, Shi Guo-Ping, Kuzuya Masafumi, Cheng Xian Wu

    JOURNAL OF HYPERTENSION   38 巻 ( 8 ) 頁: 1514 - 1524   2020年8月

     詳細を見る

    記述言語:日本語   出版者・発行元:Journal of Hypertension  

    Background:Chronic psychological stress (CPS) is linked to cardiovascular disease initiation and progression. Given that cysteinyl cathepsin K (CatK) participates in vascular remodeling and atherosclerotic plaque growth in several animal models, we investigated the role of CatK in the development of experimental neointimal hyperplasia in response to chronic stress.Methods and results:At first, male wild-type (CatK+/+) mice that underwent carotid ligation injury were subjected to chronic immobilization stress. On postoperative and stressed day 14, the results demonstrated that stress accelerated injury-induced neointima hyperplasia. On day 4, stressed mice showed following: increased levels of monocyte chemoattractant protein-1, gp91phox, toll-like receptor-2 (TLR2), TLR4, and CatK mRNAs or/and proteins, oxidative stress production, aorta-derived smooth muscle cell (SMC) migration, and macrophage infiltration as well as targeted intracellular proliferating-related molecules. Stressed mice showed increased matrix metalloproteinase-2 (MMP-2) and MMP-9 mRNA expressions and activities and elastin disruption in the injured carotid arteries. Second, CatK+/+and CatK deficiency (CatK-/-) mice received ligation injury and stress to explore the role of CatK. The stress-induced harmful changes were prevented by CatK-/-. Finally, CatK+/+mice that had undergone ligation surgery were randomly assigned to one of two groups and administered vehicle or CatK inhibitor for 14 days. Pharmacological CatK intervention produced a vascular benefit.Conclusion:These data indicate that CatK deletion protects against the development of experimental neointimal hyperplasia via the attenuation of inflammatory overaction, oxidative stress production, and VSMC proliferation, suggesting that CatK is a novel therapeutic target for the management of CPS-related restenosis after intravascular intervention therapies.

    DOI: 10.1097/HJH.0000000000002424

    Web of Science

    Scopus

    PubMed

  9. Increased dipeptidyl peptidase-4 accelerates chronic stress-related thrombosis in a mouse carotid artery model 査読有り

    Jin Xianglan, Jin Chunzi, Nakamura Kae, Jin Tiefeng, Xin Minglong, Wan Ying, Yue Xueling, Jin Shengyu, Wang Hailong, Inoue Aiko, Nan Yongshan, Lin Zhenhua, Kuzuya Masafumi, Cheng Xian Wu

    JOURNAL OF HYPERTENSION   38 巻 ( 8 ) 頁: 1504 - 1513   2020年8月

     詳細を見る

    記述言語:日本語   出版者・発行元:Journal of Hypertension  

    Objective:Exposure to chronic psychosocial stress is a risk factor for metabolic cardiovascular disorders. Given that dipeptidyl peptidase-4 (DPP-4) has an important role in human pathobiology, we investigated the role of DPP-4 in stress-related thrombosis in mice, focusing on oxidative stress and the von Willebrand factor (vWF)-cleaving protease ADAMTS13 (a disintegrin and metalloproteinase with thrombospondin type 1 motif, member 13).Methods and results:Male mice randomly assigned to nonstress and 2-week immobilized-stress groups underwent iron chloride3 (FeCl3)-induced carotid artery thrombosis surgery for morphological and biochemical studies at specific times. On day 14 post-stress/surgery, stress had enhanced the lengths and weights of arterial thrombi, with alterations of plasma DPP-4, plasminogen activation inhibitor-1 and ADAMTS13. The stressed mice had increased levels of vascular cell adhesion molecule-1, intracellular adhesion molecule-1, monocyte chemoattractant protein-1, gp91phox, p22phox, matrix metalloproteinase-2 (MMP-2), MMP-9, cathepsins S and K mRNAs and/or proteins, and reduced levels of endothelial nitric oxide synthase, catalase and superoxide dismutase-1 mRNAs and/or proteins. Stress also accelerated arterial endothelial cell damage. The DPP-4 inhibitor anagliptin ameliorated the stress-induced targeted molecular and morphological changes and thrombosis. In vitro, DPP-4 inhibition also mitigated the alterations in the targeted ADAMTS13 and other oxidative and inflammatory molecules in human umbilical vein endothelial cells in response to H2O2.Conclusion:DPP-4 inhibition appeared to improve the FeCl3-induced thrombosis in mice that received stress, possibly via the improvement of ADAMTS13 and oxidative stress, suggesting that DPP-4 could become a novel therapeutic target for chronic psychological stress-related thrombotic events in metabolic cardiovascular disorders.

    DOI: 10.1097/HJH.0000000000002418

    Web of Science

    Scopus

    PubMed

  10. Non-thermal plasma-activated lactate solution kills U251SP glioblastoma cells in an innate reductive manner with altered metabolism 査読有り

    Ishikawa Kenji, Hosoi Yugo, Tanaka Hiromasa, Jiang Li, Toyokuni Shinya, Nakamura Kae, Kajiyama Hiroaki, Kikkawa Fumitaka, Mizuno Masaaki, Hori Masaru

    ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS   688 巻   頁: 108414   2020年7月

     詳細を見る

    記述言語:日本語   出版者・発行元:Archives of Biochemistry and Biophysics  

    Ringer's lactate solution irradiated by non-thermal plasma, comprised of radicals, electrons, and ions, is defined as plasma-activated lactate (PAL). PAL exhibited antitumor effects in glioblastoma U251SP cells, which we termed PAL-specific regulated cell death. In contrast to the oxidative stress condition typical of cells incubated in plasma-activated medium (PAM), U251SP cells treated with Ringer's lactate solution or PAL exhibited changes in intracellular metabolites that were reductive in the redox state, as measured by the ratio of oxidative/reductive glutathione concentrations. In the metabolomic profiles of PAL-treated cells, the generation of acetyl-CoA increased for lipid metabolism from alanine and asparagine. PAL thus induces regulated death of U251SP glioblastoma cells in more innate microenvironments than PAM.

    DOI: 10.1016/j.abb.2020.108414

    Web of Science

    Scopus

    PubMed

  11. Ovarian cancer-associated mesothelial cells induce acquired platinum-resistance in peritoneal metastasis via the FN1/Akt signaling pathway 査読有り

    Yoshihara Masato, Kajiyama Hiroaki, Yokoi Akira, Sugiyama Mai, Koya Yoshihiro, Yamakita Yoshihiko, Liu Wenting, Nakamura Kae, Moriyama Yoshinori, Yasui Hiroaki, Suzuki Shiro, Yamamoto Yusuke, Ricciardelli Carmela, Nawa Akihiro, Shibata Kiyosumi, Kikkawa Fumitaka

    INTERNATIONAL JOURNAL OF CANCER   146 巻 ( 8 ) 頁: 2268 - 2280   2020年4月

     詳細を見る

    記述言語:日本語   出版者・発行元:International Journal of Cancer  

    Peritoneal dissemination of ovarian cancer (OvCa) arises from the surface of the peritoneum, covered by monolayer of mesothelial cells (MCs). Given that both OvCa cells and MCs are present in the same peritoneal metastatic microenvironment, they may establish cell-to-cell crosstalk or phenotypic alterations including the acquisition of platinum-resistance in OvCa cells. Herein, we report how OvCa-associated mesothelial cells (OCAMs) induce platinum-resistance in OvCa cells through direct cell-to-cell crosstalk. We evaluated mutual associations between OvCa cells and human primary MCs with in vitro coculturing experimental models and in silico omics data analysis. The role of OCAMs was also investigated using clinical samples and in vivo mice models. Results of in vitro experiments show that mesenchymal transition is induced in OCAMs primarily by TGF-β1 stimulation. Furthermore, OCAMs influence the behavior of OvCa cells as a component of the tumor microenvironment of peritoneal metastasis. Mechanistically, OCAMs can induce decreased platinum-sensitivity in OvCa cells via induction of the FN1/Akt signaling pathway via cell-to-cell interactions. Histological analysis of OvCa peritoneal metastasis also illustrated FN1 expression in stromal cells that are supposed to originate from MCs. Further, we also confirmed the activation of Akt signaling in OvCa cells in contact with TGF-β1 stimulated peritoneum, using an in vivo mice model. Our results suggest that the tumor microenvironment, enhanced by direct cell-to-cell crosstalk between OvCa cells and OCAMs, induces acquisition of platinum-resistance in OvCa cells, which may serve as a novel therapeutic target for prevention of OvCa peritoneal dissemination.

    DOI: 10.1002/ijc.32854

    Web of Science

    Scopus

    PubMed

  12. CCL2 secreted from cancer-associated mesothelial cells promotes peritoneal metastasis of ovarian cancer cells through the P38-MAPK pathway 査読有り

    Yasui Hiroaki, Kajiyama Hiroaki, Tamauchi Satoshi, Suzuki Shiro, Peng Yang, Yoshikawa Nobuhisa, Sugiyama Mai, Nakamura Kae, Kikkawa Fumitaka

    CLINICAL & EXPERIMENTAL METASTASIS   37 巻 ( 1 ) 頁: 145 - 158   2020年2月

     詳細を見る

  13. Plasma-activated medium promotes autophagic cell death along with alteration of the mTOR pathway 査読有り

    Yoshikawa Nobuhisa, Liu Wenting, Nakamura Kae, Yoshida Kosuke, Ikeda Yoshiki, Tanaka Hiromasa, Mizuno Masaaki, Toyokuni Shinya, Hori Masaru, Kikkawa Fumitaka, Kajiyama Hiroaki

    SCIENTIFIC REPORTS   10 巻 ( 1 ) 頁: 1614   2020年1月

     詳細を見る

    記述言語:日本語   出版者・発行元:Scientific Reports  

    The biological function of non-thermal atmospheric pressure plasma has been widely accepted in several types of cancer. We previously developed plasma-activated medium (PAM) for clinical use, and demonstrated that PAM exhibits a metastasis-inhibitory effect on ovarian cancer through reduced MMP-9 secretion. However, the anti-tumor effects of PAM on endometrial cancer remain unknown. In this study, we investigated the inhibitory effect of PAM on endometrial cancer cell viability in vitro. Our results demonstrated that AMEC and HEC50 cell viabilities were reduced by PAM at a certain PAM ratio, and PAM treatment effectively increased autophagic cell death in a concentration dependent manner. In addition, we evaluated the molecular mechanism of PAM activity and found that the mTOR pathway was inactivated by PAM. Moreover, our results demonstrated that the autophagy inhibitor MHY1485 partially inhibited the autophagic cell death induced by PAM treatment. These findings indicate that PAM decreases the viability of endometrial cancer cells along with alteration of the mTOR pathway, which is critical for cancer cell viability. Collectively, our data suggest that PAM inhibits cell viability while inducing autophagic cell death in endometrial cancer cells, representing a potential novel treatment for endometrial cancer.

    DOI: 10.1038/s41598-020-58667-3

    Web of Science

    Scopus

    PubMed

  14. Oxidative stress-dependent and -independent death of glioblastoma cells induced by non-thermal plasma-exposed solutions 査読有り

    Tanaka Hiromasa, Mizuno Masaaki, Katsumata Yuko, Ishikawa Kenji, Kondo Hiroki, Hashizume Hiroshi, Okazaki Yasumasa, Toyokuni Shinya, Nakamura Kae, Yoshikawa Nobuhisa, Kajiyama Hiroaki, Kikkawa Fumitaka, Hori Masaru

    SCIENTIFIC REPORTS   9 巻 ( 1 ) 頁: 13657   2019年9月

     詳細を見る

  15. Cathepsin S Deficiency Mitigated Chronic Stress-Related Neointimal Hyperplasia in Mice 査読有り

    Wang Hailong, Meng Xiangkun, Piao Limei, Inoue Aiko, Xu Wenhu, Yu Chenglin, Nakamura Kae, Hu Lina, Sasaki Takeshi, Wu Hongxian, Unno Kazumasa, Umegaki Hiroyuki, Murohara Toyoaki, Shi Guo-Ping, Kuzuya Masafumi, Cheng Xian Wu

    JOURNAL OF THE AMERICAN HEART ASSOCIATION   8 巻 ( 14 ) 頁: e011994   2019年7月

     詳細を見る

    記述言語:日本語  

    DOI: 10.1161/JAHA.119.011994

    Web of Science

    PubMed

  16. Non-thermal plasma-activated medium modified metabolomic profiles in the glycolysis of U251SP glioblastoma 査読有り

    Kurake Naoyuki, Ishikawa Kenji, Tanaka Hiromasa, Hashizume Hiroshi, Nakamura Kae, Kajiyama Hiroaki, Toyokuni Shinya, Kikkawa Fumitaka, Mizuno Masaaki, Hori Masaru

    ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS   662 巻   頁: 83 - 92   2019年2月

     詳細を見る

  17. PAI-1 secreted from metastatic ovarian cancer cells triggers the tumor-promoting role of the mesothelium in a feedback loop to accelerate peritoneal dissemination 査読有り

    Peng Yang, Kajiyama Hiroaki, Yuan Hong, Nakamura Kae, Yoshihara Masato, Yokoi Akira, Fujikake Kayo, Yasui Hiroaki, Yoshikawa Nobuhisa, Suzuki Shiro, Senga Takeshi, Shibata Kiyosumi, Kikkawa Fumitaka

    CANCER LETTERS   442 巻   頁: 181 - 192   2019年2月

     詳細を見る

  18. Effects of exosomes derived from the induced pluripotent stem cells on skin wound healing 査読有り

    Kobayashi Hitoshi, Ebisawa Katsumi, Kambe Miki, Kasai Takatoshi, Suga Hidetaka, Nakamura Kae, Narita Yuji, Ogata Aika, Kamei Yuzuru

    NAGOYA JOURNAL OF MEDICAL SCIENCE   80 巻 ( 2 ) 頁: 141 - 153   2018年5月

     詳細を見る

  19. Connective tissue growth factor-specific monoclonal antibody inhibits growth of malignant mesothelioma in an orthotopic mouse model. 査読有り

    Ohara Y, Chew SH, Misawa N, Wang S, Somiya D, Nakamura K, Kajiyama H, Kikkawa F, Tsuyuki Y, Jiang L, Yamashita K, Sekido Y, Lipson KE, Toyokuni S

    Oncotarget   9 巻 ( 26 ) 頁: 18494 - 18509   2018年4月

     詳細を見る

    記述言語:英語  

    DOI: 10.18632/oncotarget.24892

    PubMed

  20. Glioblastoma Cell Lines Display Different Sensitivities to Plasma-Activated Medium 査読有り

    Tanaka Hiromasa, Mizuno Masaaki, Ishikawa Kenji, Takeda Keigo, Hashizume Hiroshi, Nakamura Kae, Utsumi Fumi, Kajiyama Hiroaki, Okazaki Yasumasa, Toyokuni Shinya, Akiyama Shinichi, Maruyama Shoichi, Kikkawa Fumitaka, Hori Masaru

    IEEE TRANSACTIONS ON RADIATION AND PLASMA MEDICAL SCIENCES   2 巻 ( 2 ) 頁: 99 - 102   2018年3月

     詳細を見る

    記述言語:日本語  

    DOI: 10.1109/TRPMS.2017.2721973

    Web of Science

  21. Effect of Plasma-Activated Lactated Ringer's Solution on Pancreatic Cancer Cells In Vitro and In Vivo 査読有り

    Sato Yusuke, Yamada Suguru, Takeda Shigeomi, Hattori Norifumi, Nakamura Kae, Tanaka Hiromasa, Mizuno Masaaki, Hori Masaru, Kodera Yasuhiro

    ANNALS OF SURGICAL ONCOLOGY   25 巻 ( 1 ) 頁: 299 - 307   2018年1月

     詳細を見る

  22. Crystallization of calcium oxalate dihydrate in a buffered calcium-containing glucose solution by irradiation with non-equilibrium atmospheric pressure plasma 査読有り

    Kurake Naoyuki, Tanaka Hiromasa, Ishikawa Kenji, Nakamura Kae, Kajiyama Hiroaki, Kikkawa Fumitaka, Mizuno Masaaki, Ikehara Yuzuru, Hori Masaru

    JOURNAL OF APPLIED PHYSICS   122 巻 ( 14 )   2017年10月

     詳細を見る

    記述言語:日本語  

    DOI: 10.1063/1.5006598

    Web of Science

  23. Intraperitoneal Administration of Plasma-Activated Medium: Proposal of a Novel Treatment Option for Peritoneal Metastasis From Gastric Cancer 査読有り

    Takeda Shigeomi, Yamada Suguru, Hattori Norifumi, Nakamura Kae, Tanaka Hiromasa, Kajiyama Hiroaki, Kanda Mitsuro, Kobayashi Daisuke, Tanaka Chie, Fujii Tsutomu, Fujiwara Michitaka, Mizuno Masaaki, Hori Masaru, Kodera Yasuhiro

    ANNALS OF SURGICAL ONCOLOGY   24 巻 ( 5 ) 頁: 1188 - 1194   2017年5月

     詳細を見る

  24. Effects of center dot OH and center dot NO radicals in the aqueous phase on H2O2 and NO2- generated in plasma-activated medium 査読有り

    Kurake Naoyuki, Tanaka Hiromasa, Ishikawa Kenji, Takeda Keigo, Hashizume Hiroshi, Nakamura Kae, Kajiyama Hiroaki, Kondo Takashi, Kikkawa Fumitaka, Mizuno Masaaki, Hori Masaru

    JOURNAL OF PHYSICS D-APPLIED PHYSICS   50 巻 ( 15 )   2017年3月

     詳細を見る

    記述言語:日本語  

    DOI: 10.1088/1361-6463/aa5f1d

    Web of Science

  25. Future perspective of strategic non-thermal plasma therapy for cancer treatment 招待有り 査読有り

    Kajiyama Hiroaki, Utsumi Fumi, Nakamura Kae, Tanaka Hiromasa, Toyokuni Shinya, Hori Masaru, Kikkawa Fumitaka

    JOURNAL OF CLINICAL BIOCHEMISTRY AND NUTRITION   60 巻 ( 1 ) 頁: 33 - 38   2017年1月

     詳細を見る

    記述言語:日本語   出版者・発行元:日本酸化ストレス学会  

    <p>The therapeutic effects of non-thermal plasma are expected in the medical fields, including hemostasis, vascularization, prevention of organ adhesion, and cell proliferation. Cancer is an internal enemy arising from normal tissue in the body. The prognosis of metastatic and recurrent cancers is still poor despite advances in medicine. To apply non-thermal plasma in cancer treatment is now on going. The mechanism of the proliferation-inhibitory effect of plasma is reactive nitrogen oxide species/reactive oxygen species production in cells. There are a number of problems to be overcome, such as existence of intrinsic reactive oxygen species/reactive nitrogen species scavengers and the shallow infiltration of plasma on tumor surface. The current reviews makes referral to the study results of plasma therapy clarified so far, the possibility of its application in the future.</p>

    DOI: 10.3164/jcbn.16-65

    Web of Science

    PubMed

    CiNii Article

  26. PRIMA-1 MET induces apoptosis through accumulation of intracellular reactive oxygen species irrespective of p53 status and chemo-sensitivity in epithelial ovarian cancer cells 査読有り

    Yoshikawa N., Kajiyama, H., Nakamura, K., Utsumi, F., Niimi, K., Mitsui, H., Sekiya, R., Suzuki, S., Shibata, K., Callen, D., Kikkawa, F.

    Oncology Reports   27 巻   頁: 84 - 84   2016年5月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Web of Science

▼全件表示

講演・口頭発表等 1

  1. プラズマによるがん克服への挑戦 招待有り

    中村香江

    第32回 自然科学研究機構シンポジウム 生命科学とプラズマ工学が作る未来  2021年8月21日  自然科学研究機構

     詳細を見る

    記述言語:日本語   会議種別:公開講演,セミナー,チュートリアル,講習,講義等  

    開催地:オンライン  

科研費 2

  1. 大気圧プラズマによる微小環境を標的とした難治性腹膜播種制御機構の解明

    研究課題/研究課題番号:20K09640  2020年4月 - 2023年3月

    科学研究費助成事業  基盤研究(C)

    中村 香江, 田中 宏昌, 芳川 修久

      詳細を見る

    担当区分:研究代表者 

    配分額:4420000円 ( 直接経費:3400000円 、 間接経費:1020000円 )

    卵巣がんにおける腹膜播種は難治性・治療抵抗性の象徴的な病態として着目されており、がん細胞と腹膜微小環境に存在する腹膜中皮細胞やその他の免疫系細胞との細胞間相互作用が腹膜播種の形成・進展に重要な役割を担っていることが明らかになっている。本研究において、大気圧プラズマの抗腫瘍活性を液体化したプラズマ活性液が誘導する腹膜微小環境における腫瘍抑制機構を解明し、新規治療技術としての基礎的知見の集積を目指す。

  2. プラズマ溶液中の生理活性物質の同定ならびに生体応答機構の解明

    研究課題/研究課題番号:15K04713  2015年10月 - 2018年3月

    科学研究費助成事業  基盤研究(C)

    中村 香江

      詳細を見る

    担当区分:研究代表者 

    配分額:4550000円 ( 直接経費:3500000円 、 間接経費:1050000円 )