Language：English   Publisher：MicrobiologyOpen  

The fission yeast Schizosaccharomyces pombe employs two main strategies to adapt to the environment and survive when starved for nutrients. The strategies employ sporulation via sexual differentiation and extension of the chronological lifespan. When a cell is exposed to nutrient starvation in the presence of a cell of the opposite sex, the cells undergo fusion through conjugation and sporulation through meiosis. S. pombe spores are highly resistant to diverse stresses and may survive for a very long time. In this minireview, among the various sexual differentiation processes induced by starvation, we focused on and summarized the findings of the molecular mechanisms of spore formation in fission yeast. Furthermore, comparative measurements of the chronological lifespan of stationary phase cells and G0 cells and the survival period of spore cells revealed that the spore cells survived for a long period, indicating the presence of an effective mechanism for survival. Currently, many molecules involved in sporulation and their functions are being discovered; however, our understanding of these is not complete. Further understanding of spores may not only deepen our comprehension of sexual differentiation but may also provide hints for sustaining life.

DOI： 10.1002/mbo3.1303

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Language：English   Publisher：Bioscience, Biotechnology and Biochemistry  

Tschimganine inhibits growth and extends the chronological lifespan in Schizosaccharomyces pombe. We synthesized a Tschimganine analog, Mochimganine, which extends the lifespan similar to Tschimganine but exhibits a significantly weaker growth inhibition effect. Based on the comparative analysis of these compounds, we propose that Tschimganine has at least 2 targets: one extends the lifespan and the other inhibits growth.

DOI： 10.1093/bbb/zbac051

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Language：English  

DOI： 10.1093/femsyr/foac020

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Language：English   Publisher：Applied Microbiology, Molecular and Cellular Biosciences Research Foundation  

<p>Fission yeast, <i>Schizosaccharomyces pombe</i>, possesses eight hexose transporters, Ght1~8. In order to clarify the role of each hexose transporter on glucose uptake, a glucose uptake assay system was established and the actual glucose uptake activity of each hexose transporter-deletion mutant was measured. Under normal growth condition containing 2% glucose, ∆<i>ght5</i> and ∆<i>ght2</i> mutants showed large and small decrease in glucose uptake activity, respectively.<b> </b>On the other hand, the other deletion mutants did not show any decrease in glucose uptake activity indicating that, in the presence of Ght5 and Ght2, the other hexose transporters do not play a significant role in glucose uptake. To understand the relevance between glucose uptake and lifespan regulation, we measured the chronological lifespan of each hexose transporter deletion mutant, and found that only ∆<i>ght5</i> mutant showed a significant lifespan extension. Based on these results we showed that Ght5 is mainly involved in the glucose uptake in <i>Schizosaccharomyces pombe</i>, and suggested that the ∆<i>ght5</i> mutant has prolonged lifespan due to physiological changes similar to calorie restriction.</p>

DOI： 10.2323/jgam.2022.05.006

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CiNii Research

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1111/gtc.12897

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Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1093/femsyr/foab041

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Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1111/gtc.12854

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Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1093/femsle/fnab070

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Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1111/mmi.14627

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Language：English   Publisher：MicrobiologyOpen  

Nutrients including glucose, nitrogen, sulfur, zinc, and iron are involved in the regulation of chronological lifespan (CLS) of yeast, which serves as a model of the lifespan of differentiated cells of higher organisms. Herein, we show that magnesium (Mg2+) depletion extends CLS of the fission yeast Schizosaccharomyces pombe through a mechanism involving the Ecl1 gene family. We discovered that ecl1+ expression, which extends CLS, responds to Mg2+ depletion. Therefore, we investigated the underlying intracellular responses. In amino acid auxotrophic strains, Mg2+ depletion robustly induces ecl1+ expression through the activation of the general amino acid control (GAAC) pathway—the equivalent of the amino acid response of mammals. Polysome analysis indicated that the expression of Ecl1 family genes was required for regulating ribosome amount when cells were starved, suggesting that Ecl1 family gene products control the abundance of ribosomes, which contributes to longevity through the activation of the evolutionarily conserved GAAC pathway. The present study extends our understanding of the cellular response to Mg2+ depletion and its influence on the mechanism controlling longevity.

DOI： 10.1002/mbo3.1176

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Language：English   Publishing type：Research paper (scientific journal)  

Language：English   Publishing type：Research paper (scientific journal)  

Language：English   Publisher：Genes to Cells  

Autophagy is an intracellular degradation system widely conserved among various species. Autophagy is induced by the depletion of various nutrients, and this degradation mechanism is essential for adaptation to such conditions. In this study, we demonstrated that sulfur depletion induces autophagy in the fission yeast Schizosaccharomyces pombe. Based on the finding that autophagy induced by sulfur depletion was completely abolished in a mutant in which the ecl1, ecl2 and ecl3 genes were deleted (Δecls), we report that these three genes are essential for the induction of autophagy by sulfur depletion. Furthermore, autophagy-defective mutant cells exhibited poor growth and short lifespan (compared with wild-type cells) under the sulfur-depleted condition. These results indicated that the mechanism of autophagy is necessary for the appropriate adaptation to sulfur depletion.

DOI： 10.1111/gtc.12815

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Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1080/09168451.2019.1676695

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Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1007/s00438-019-01592-6

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Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1111/gtc.12604

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Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1111/mmi.13686

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Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1007/s00438-016-1285-x

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Event date： 2020.9

Presentation type：Oral presentation (general)  

Venue：Web開催   Country：Japan  

Event date： 2020.9

Language：Japanese   Presentation type：Poster presentation  

Venue：Web開催   Country：Japan  

Event date： 2020.9

Language：Japanese   Presentation type：Poster presentation  

Venue：Web開催   Country：Japan  

Event date： 2020.9

Language：Japanese   Presentation type：Poster presentation  

Venue：Web開催   Country：Japan  

Event date： 2020.9

Language：Japanese   Presentation type：Poster presentation  

Venue：Web開催   Country：Japan  

Event date： 2020.3

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：名古屋   Country：Japan  

Event date： 2020.3

Language：Japanese   Presentation type：Poster presentation  

Venue：名古屋   Country：Japan  

Event date： 2020.3

Language：Japanese   Presentation type：Poster presentation  

Venue：名古屋   Country：Japan  

Event date： 2020.3

Language：Japanese   Presentation type：Poster presentation  

Venue：名古屋   Country：Japan  

Event date： 2019.12

Language：Japanese   Presentation type：Poster presentation  

Venue：福岡   Country：Japan  

Event date： 2019.9

Language：Japanese   Presentation type：Poster presentation  

Venue：静岡市清水文化会館マリナート  

Event date： 2019.9

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：静岡市清水文化会館マリナート   Country：Japan  

Event date： 2019.7

Language：English   Presentation type：Poster presentation  

Venue：Barcelona, Spain   Country：Spain