Updated on 2021/11/02

写真a

 
SHIMASAKI Takafumi
 
Organization
Graduate School of Pharmaceutical Sciences Department of Basic Medicinal Sciences Assistant Professor
Graduate School
Graduate School of Pharmaceutical Sciences
Undergraduate School
School of Agricultural Sciences Department of Applied Biosciences
Title
Assistant Professor
Contact information
メールアドレス

Degree 1

  1. 博士(創薬科学) ( 2017.3   名古屋大学 ) 

Research History 1

  1. Nagoya University   School of Agricultural Sciences

    2018.4

Professional Memberships 4

  1. 日本ゲノム微生物学会

  2. 日本分子生物学会

  3. 日本農芸化学会

  4. 酵母遺伝学フォーラム

 

Papers 14

  1. Identification of ksg1 mutation showing long-lived phenotype in fission yeast Reviewed

    Matsui Kotaro, Okamoto Keisuke, Hasegawa Tomoka, Ohtsuka Hokuto, Shimasaki Takafumi, Ihara Kunio, Goto Yuhei, Aoki Kazuhiro, Aiba Hirofumi

    GENES TO CELLS     2021.9

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    Language:Japanese   Publisher:Genes to Cells  

    Fission yeast is a good model organism for the study of lifespan. To elucidate the mechanism, we screened for long-lived mutants. We found a nonsense mutation in the ksg1+ gene, which encodes an ortholog of mammalian PDK1 (phosphoinositide-dependent protein kinase). The mutation was in the PH domain of Ksg1 and caused defect in membrane localization and protein stability. Analysis of the ksg1 mutant revealed that the reduced amounts and/or activity of the Ksg1 protein are responsible for the increased lifespan. Ksg1 is essential for growth and known to phosphorylate multiple substrates, but the substrate responsible for the long-lived phenotype of ksg1 mutation is not yet known. Genetic analysis showed that deletion of pck2 suppressed the long-lived phenotype of ksg1 mutant, suggesting that Pck2 might be involved in the lifespan extension caused by ksg1 mutation.

    DOI: 10.1111/gtc.12897

    Web of Science

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  2. Response to sulfur in Schizosaccharomyces pombe Reviewed

    Ohtsuka Hokuto, Shimasaki Takafumi, Aiba Hirofumi

    FEMS YEAST RESEARCH   Vol. 21 ( 5 )   2021.8

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    Language:Japanese   Publisher:FEMS Yeast Research  

    Sulfur is an essential component of various biologically important molecules, including methionine, cysteine and glutathione, and it is also involved in coping with oxidative and heavy metal stress. Studies using model organisms, including budding yeast (Saccharomyces cerevisiae) and fission yeast (Schizosaccharomyces pombe), have contributed not only to understanding various cellular processes but also to understanding the utilization and response mechanisms of each nutrient, including sulfur. Although fission yeast can use sulfate as a sulfur source, its sulfur metabolism pathway is slightly different from that of budding yeast because it does not have a trans-sulfuration pathway. In recent years, it has been found that sulfur starvation causes various cellular responses in S. pombe, including sporulation, cell cycle arrest at G2, chronological lifespan extension, autophagy induction and reduced translation. This MiniReview identifies two sulfate transporters in S. pombe, Sul1 (encoded by SPBC3H7.02) and Sul2 (encoded by SPAC869.05c), and summarizes the metabolic pathways of sulfur assimilation and cellular response to sulfur starvation. Understanding these responses, including metabolism and adaptation, will contribute to a better understanding of the various stress and nutrient starvation responses and chronological lifespan regulation caused by sulfur starvation.

    DOI: 10.1093/femsyr/foab041

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  3. Extension of chronological lifespan in Schizosaccharomyces pombe Reviewed

    Ohtsuka Hokuto, Shimasaki Takafumi, Aiba Hirofumi

    GENES TO CELLS   Vol. 26 ( 7 ) page: 459 - 473   2021.7

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    Language:Japanese   Publisher:Genes to Cells  

    There are several examples in the nature wherein the mechanism of longevity control of unicellular organisms is evolutionarily conserved with that of higher multicellular organisms. The present microreview focuses on aging and longevity studies, particularly on chronological lifespan (CLS) concerning the unicellular eukaryotic fission yeast Schizosaccharomyces pombe. In S. pombe, >30 compounds, 8 types of nutrient restriction, and >80 genes that extend CLS have been reported. Several CLS control mechanisms are known to be involved in nutritional response, energy utilization, stress responses, translation, autophagy, and sexual differentiation. In unicellular organisms, the control of CLS is directly linked to the mechanism by which cells are maintained in limited-resource environments, and their genetic information is left to posterity. We believe that this important mechanism may have been preserved as a lifespan control mechanism for higher organisms.

    DOI: 10.1111/gtc.12854

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  4. Identification of sur2 mutation affecting the lifespan of fission yeast Reviewed

    Kurauchi Tatsuhiro, Matsui Kotaro, Shimasaki Takafumi, Ohtsuka Hokuto, Tsubouchi Satoshi, Ihara Kunio, Tani Motohiro, Aiba Hirofumi

    FEMS MICROBIOLOGY LETTERS   Vol. 368 ( 12 )   2021.6

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    Language:Japanese   Publisher:FEMS Microbiology Letters  

    Yeast is a suitable model system to analyze the mechanism of lifespan. In this study, to identify novel factors involved in chronological lifespan, we isolated a mutant with a long chronological lifespan and found a missense mutation in the sur2+ gene, which encodes a homolog of Saccharomyces cerevisiae sphingolipid C4-hydroxylase in fission yeast. Characterization of the mutant revealed that loss of sur2 function resulted in an extended chronological lifespan. The effect of caloric restriction, a well-known signal for extending lifespan, is thought to be dependent on the sur2+ gene.

    DOI: 10.1093/femsle/fnab070

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  5. Genes affecting the extension of chronological lifespan in Schizosaccharomyces pombe (fission yeast) Reviewed

    Ohtsuka Hokuto, Shimasaki Takafumi, Aiba Hirofumi

    MOLECULAR MICROBIOLOGY   Vol. 115 ( 4 ) page: 623 - 642   2021.4

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    Language:Japanese   Publisher:Molecular Microbiology  

    So far, more than 70 genes involved in the chronological lifespan (CLS) of Schizosaccharomyces pombe (fission yeast) have been reported. In this mini-review, we arrange and summarize these genes based on the reported genetic interactions between them and the physical interactions between their products. We describe the signal transduction pathways that affect CLS in S. pombe: target of rapamycin complex 1, cAMP-dependent protein kinase, Sty1, and Pmk1 pathways have important functions in the regulation of CLS extension. Furthermore, the Php transcription complex, Ecl1 family proteins, cyclin Clg1, and the cyclin-dependent kinase Pef1 are important for the regulation of CLS extension in S. pombe. Most of the known genes involved in CLS extension are related to these pathways and genes. In this review, we focus on the individual genes regulating CLS extension in S. pombe and discuss the interactions among them.

    DOI: 10.1111/mmi.14627

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  6. Magnesium depletion extends fission yeast lifespan via general amino acid control activation Reviewed

    Ohtsuka Hokuto, Kobayashi Mikuto, Shimasaki Takafumi, Sato Teppei, Akanuma Genki, Kitaura Yasuyuki, Otsubo Yoko, Yamashita Akira, Aiba Hirofumi

    MICROBIOLOGYOPEN   Vol. 10 ( 2 ) page: e1176   2021.3

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    Language:Japanese   Publisher:MicrobiologyOpen  

    Nutrients including glucose, nitrogen, sulfur, zinc, and iron are involved in the regulation of chronological lifespan (CLS) of yeast, which serves as a model of the lifespan of differentiated cells of higher organisms. Herein, we show that magnesium (Mg2+) depletion extends CLS of the fission yeast Schizosaccharomyces pombe through a mechanism involving the Ecl1 gene family. We discovered that ecl1+ expression, which extends CLS, responds to Mg2+ depletion. Therefore, we investigated the underlying intracellular responses. In amino acid auxotrophic strains, Mg2+ depletion robustly induces ecl1+ expression through the activation of the general amino acid control (GAAC) pathway—the equivalent of the amino acid response of mammals. Polysome analysis indicated that the expression of Ecl1 family genes was required for regulating ribosome amount when cells were starved, suggesting that Ecl1 family gene products control the abundance of ribosomes, which contributes to longevity through the activation of the evolutionarily conserved GAAC pathway. The present study extends our understanding of the cellular response to Mg2+ depletion and its influence on the mechanism controlling longevity.

    DOI: 10.1002/mbo3.1176

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  7. Magnesium depletion extends fission yeast lifespan via general amino acid control activation Reviewed

    MicrobiologyOpen     2021

  8. Sulfur depletion induces autophagy through Ecl1 family genes in fission yeast Reviewed

    Shimasaki Takafumi, Okamoto Keisuke, Ohtsuka Hokuto, Aiba Hirofumi

    GENES TO CELLS   Vol. 25 ( 12 ) page: 825 - 830   2020.12

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    Language:Japanese   Publisher:Genes to Cells  

    Autophagy is an intracellular degradation system widely conserved among various species. Autophagy is induced by the depletion of various nutrients, and this degradation mechanism is essential for adaptation to such conditions. In this study, we demonstrated that sulfur depletion induces autophagy in the fission yeast Schizosaccharomyces pombe. Based on the finding that autophagy induced by sulfur depletion was completely abolished in a mutant in which the ecl1, ecl2 and ecl3 genes were deleted (Δecls), we report that these three genes are essential for the induction of autophagy by sulfur depletion. Furthermore, autophagy-defective mutant cells exhibited poor growth and short lifespan (compared with wild-type cells) under the sulfur-depleted condition. These results indicated that the mechanism of autophagy is necessary for the appropriate adaptation to sulfur depletion.

    DOI: 10.1111/gtc.12815

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  9. gas1 mutation extends chronological lifespan via Pmk1 and Sty1 MAPKs in Schizosaccharomyces pombe Reviewed

    Imai Yuki, Shimasaki Takafumi, Enokimura Chihiro, Ohtsuka Hokuto, Tsubouchi Satoshi, Ihara Kunio, Aiba Hirofumi

    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY   Vol. 84 ( 2 ) page: 330 - 337   2020.2

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    Language:Japanese   Publishing type:Research paper (scientific journal)  

    DOI: 10.1080/09168451.2019.1676695

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  10. Leucine depletion extends the lifespans of leucine-auxotrophic fission yeast by inducing Ecl1 family genes via the transcription factor Fil1 Reviewed

    Ohtsuka Hokuto, Kato Takanori, Sato Teppei, Shimasaki Takafumi, Kojima Takaaki, Aiba Hirofumi

    MOLECULAR GENETICS AND GENOMICS   Vol. 294 ( 6 ) page: 1499 - 1509   2019.12

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    Language:Japanese   Publishing type:Research paper (scientific journal)  

    DOI: 10.1007/s00438-019-01592-6

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  11. Tschimganine and its derivatives extend the chronological life span of yeast via activation of the Sty1 pathway Reviewed

    Hibi Takahide, Ohtsuka Hokuto, Shimasaki Takafumi, Inui Shougo, Shibuya Masatoshi, Tatsukawa Hideki, Kanie Kei, Yamamoto Yoshihiko, Aiba Hirofumi

    GENES TO CELLS   Vol. 23 ( 8 ) page: 620 - 637   2018.8

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    Language:Japanese   Publishing type:Research paper (scientific journal)  

    DOI: 10.1111/gtc.12604

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  12. Sulfur restriction extends fission yeast chronological lifespan through Ecl1 family genes by downregulation of ribosome Reviewed

    Ohtsuka Hokuto, Takinami Masahiro, Shimasaki Takafumi, Hibi Takahide, Murakami Hiroshi, Aiba Hirofumi

    MOLECULAR MICROBIOLOGY   Vol. 105 ( 1 ) page: 84 - 97   2017.7

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    Language:Japanese   Publishing type:Research paper (scientific journal)  

    DOI: 10.1111/mmi.13686

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  13. Ecl1 is a zinc-binding protein involved in the zinc-limitation-dependent extension of chronological life span in fission yeast Reviewed

    Shimasaki Takafumi, Ohtsuka Hokuto, Naito Chikako, Azuma Kenko, Tenno Takeshi, Hiroaki Hidekazu, Murakami Hiroshi, Aiba Hirofumi

    MOLECULAR GENETICS AND GENOMICS   Vol. 292 ( 2 ) page: 475 - 481   2017.4

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    Language:Japanese   Publishing type:Research paper (scientific journal)  

    DOI: 10.1007/s00438-016-1285-x

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  14. Ecl1 is activated by the transcription factor Atf1 in response to H2O2 stress in Schizosaccharomyces pombe Reviewed

        2014.4

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Presentations 18

  1. 分裂酵母における(Ecl1ファミリー遺伝子を介した)硫黄枯渇による細胞小型化の解析

    八田佳子, 筒井優, 服部允赳, 島崎崇史, 大塚北斗, 饗場浩文

    第42回 日本分子生物学会年会  

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    Event date: 2020.12

    Language:Japanese   Presentation type:Poster presentation  

    Venue:福岡   Country:Japan  

  2. Mg枯渇時における分裂酵母の経時寿命延長因子Ecl1 Family遺伝子の解析

    小林未来登, 佐藤哲平, 大塚北斗, 島崎嵩史, 饗場浩文

    日本農芸化学会中部支部第187回例会  

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    Event date: 2020.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:Web開催   Country:Japan  

  3. 分裂酵母におけるGhtファミリータンパク質の欠失がグルコース取り込みと寿命へ与える影響の解析

    丸山哲平、林可奈子、島崎嵩史、大塚北斗、齋藤成昭、饗場浩文

    日本農芸化学会中部支部第187回例会 

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    Event date: 2020.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:Web開催   Country:Japan  

  4. 分裂酵母におけるTschimganineの作用機構の解析

    松本拓磨、大塚北斗、持田尚宏、島崎嵩史、澁谷正俊、山本芳彦、饗場浩文

    日本農芸化学会中部支部第187回例会 

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    Event date: 2020.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:Web開催   Country:Japan  

  5. 分裂酵母における硫黄枯渇制限下での細胞小型化の解析

    八田佳子、筒井優、服部允赳、島崎嵩史、大塚北斗、饗場浩文

    日本農芸化学会中部支部第187回例会 

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    Event date: 2020.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:Web開催   Country:Japan  

  6. 分裂酵母において硫黄枯渇はEcl1ファミリー遺伝子依存的にオートファジーを誘導する

    島崎嵩史, 岡本啓佑, 大塚北斗, 饗場浩文

    酵母遺伝学フォーラム第53回研究報告会 

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    Event date: 2020.9

    Presentation type:Oral presentation (general)  

    Venue:Web開催   Country:Japan  

  7. 分裂酵母におけるTschimganineの作用機構の解析

    松本拓磨、大塚北斗、持田尚宏、島崎嵩史、山本芳彦、饗場浩文

    酵母遺伝学フォーラム第53回研究報告会  

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    Event date: 2020.9

    Language:Japanese   Presentation type:Poster presentation  

    Venue:Web開催   Country:Japan  

  8. Mg枯渇時における分裂酵母の経時寿命延長因子Ecl1ファミリー遺伝子の解析

    小林未来登, 佐藤哲平, 大塚北斗, 島崎嵩史, 饗場浩文

    酵母遺伝学フォーラム第53回研究報告会  

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    Event date: 2020.9

    Language:Japanese   Presentation type:Poster presentation  

    Venue:Web開催   Country:Japan  

  9. 分裂酵母における硫黄枯渇による細胞小型化の解析

    八田佳子、筒井優、服部允赳、大塚北斗、島崎嵩史、饗場浩文

    酵母遺伝学フォーラム第53回研究報告会  

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    Event date: 2020.9

    Language:Japanese   Presentation type:Poster presentation  

    Venue:Web開催   Country:Japan  

  10. 経時寿命が延長する分裂酵母変異株のスクリーニングと新規寿命関連因子の同定

    松井滉太朗、岡本啓佑、長谷川朋香、島崎嵩史、大塚北斗、井原邦夫、後藤祐平、青木一洋、饗場浩文

    酵母遺伝学フォーラム第53回研究報告会  

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    Event date: 2020.9

    Language:Japanese   Presentation type:Poster presentation  

    Venue:Web開催   Country:Japan  

  11. 分裂酵母におけるアミノ酸枯渇応答機構の解析

    島崎嵩史、大塚北斗、佐藤哲平、赤沼元気、饗場浩文

    第14回 日本ゲノム微生物学会年会  

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    Event date: 2020.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:名古屋   Country:Japan  

  12. 経時寿命が延長する分裂酵母変異株のスクリーニングと新規寿命関連因子の同定

    松井滉太朗、岡本啓佑、長谷川朋香、島崎嵩史、大塚北斗、井原邦夫、中村彰伸、後藤祐平、青木一洋、饗場浩文

    第14回 日本ゲノム微生物学会年会  

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    Event date: 2020.3

    Language:Japanese   Presentation type:Poster presentation  

    Venue:名古屋   Country:Japan  

  13. 分裂酵母における硫黄枯渇と細胞応答

    筒井優、服部允赳、八田佳子、大塚北斗、島崎嵩史、饗場浩文

    第14回 日本ゲノム微生物学会年会 

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    Event date: 2020.3

    Language:Japanese   Presentation type:Poster presentation  

    Venue:名古屋   Country:Japan  

  14. 分裂酵母におけるTschimganineの作用機構の解析

    持田尚宏、大塚北斗、松本拓磨、島崎嵩史、澁谷正俊、山本芳彦、饗場浩文

    第14回 日本ゲノム微生物学会年会  

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    Event date: 2020.3

    Language:Japanese   Presentation type:Poster presentation  

    Venue:名古屋   Country:Japan  

  15. マグネシウム枯渇条件下における分裂酵母の経時寿命延長因子Ecl1 family遺伝子の解析

    小林未来登, 佐藤哲平, 大塚北斗, 島崎嵩史, 饗場浩文

    第42回 日本分子生物学会年会  

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    Event date: 2019.12

    Language:Japanese   Presentation type:Poster presentation  

    Venue:福岡   Country:Japan  

  16. アミノ酸枯渇に応答する分裂酵母の経時寿命延長因子Ecl1 Family 遺伝子の解析

    佐藤哲平、大塚北斗、加藤敬典、島崎嵩史、饗場浩文

    酵母遺伝学フォーラム第52回研究報告会  

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    Event date: 2019.9

    Language:Japanese   Presentation type:Poster presentation  

    Venue:静岡市清水文化会館マリナート  

  17. 分裂酵母におけるgas1 変異による寿命延長機構の解析

    島崎嵩史、今井優希、榎村千尋、大塚北斗、井原邦夫、饗場浩文

    酵母遺伝学フォーラム第52回研究報告会 

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    Event date: 2019.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:静岡市清水文化会館マリナート   Country:Japan  

  18. Leucine depletion extends the lifespans of fission yeast by inducing Ecl1 family genes via the transcription factor Fil1. International conference

    Hirofumi Aiba, Hokuto Ohtsuka, Takanori Kato, Teppei Sato, and Takafumi Shimasaki

    The 10 th International Fission Yeast Meeting  

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    Event date: 2019.7

    Language:English   Presentation type:Poster presentation  

    Venue:Barcelona, Spain   Country:Spain  

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KAKENHI (Grants-in-Aid for Scientific Research) 1

  1. 分裂酵母における新規キナーゼNnk1による寿命制御機構の解明

    Grant number:21K14769  2021.4 - 2023.3

    科学研究費助成事業  若手研究

    島崎 嵩史

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    Authorship:Principal investigator 

    Grant amount:\4550000 ( Direct Cost: \3500000 、 Indirect Cost:\1050000 )

    本研究では、分裂酵母における新規の寿命制御因子であるNnk1タンパク質の生理学的機能、およびその寿命制御メカニズムの解明を行う。分裂酵母は基本的な細胞内メカニズムが高等生物と類似しており、種々の寿命制御因子(カロリー制限応答、PKA、TORなど)も保存されており、優れた寿命研究モデルである。分裂酵母におけるNnk1タンパク質による寿命制御メカニズムの解明を通して、高等生物の寿命制御の理解に寄与する知見を得ることを目指す。

 

Teaching Experience (On-campus) 1

  1. 応用生命科学実験実習

    2020