研究者詳細 - 東　直輝

論文 - 東　直輝

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マイクロ流体デバイスと蛍光相関分光法の組み合わせによる一様微小隙間での潤滑剤粘度計測

木本貴幸, 福澤健二, 伊藤伸太郎, 東直輝, 張賀東

IIP情報・知能・精密機器部門講演会講演論文集 2020 巻 ( 0 ) 頁： 2C08 2020年

ナノ厚さリン脂質ポリマーブラシ膜のずり粘弾性定量化に向けた光干渉縞計測によるせん断すき間同定法

林楓昌, 伊藤伸太郎, 福澤健二, 張賀東, 東直輝

年次大会 2020 巻 ( 0 ) 頁： J13111 2020年

ナノスリット流路を電気泳動する大分子量DNAの分子伸長とその電圧依存性

永田春香, 伊藤伸太郎, 東直輝, 福澤健二, 張賀東

IIP情報・知能・精密機器部門講演会講演論文集 2020 巻 ( 0 ) 頁： 2C05 2020年

Dynamic Viscoelasticity of Hydrated Phospholipid Polymer Brush Measured by Fiber Wobbling Method 招待有り査読有り

S. Itoh, S. Aoyama, Y. Higashizaka, K. Fukuzawa, H. Zhang, N. Azuma

Proceedings of the STLE/ASME International Joint Tribology Conference 2019年10月

Gap and Shear Rate Dependence of Viscoelasticity of Lubricants with Polymer Additives Sheared in Nano Gaps 招待有り査読有り

S Itoh, R Aoki, K Fukuzawa, H Zhang, N Azuma, Y Onumata

Proceedings of the STLE/ASME International Joint Tribology Conference 2019年10月

DNA分子濃縮を用いたナノ隙間手前の分子のトラップ時間の測定

東直輝, 福澤健二, 伊藤伸太郎, 張賀東

マイクロ・ナノ工学シンポジウム 2019.10 巻 ( 0 ) 頁： 21pm1PN319 2019年

ナノスリットによる DNA 分子濃縮における理論モデルを用いた印加電圧の最適化

東直輝, 福澤健二, 伊藤伸太郎, 張賀東

IIP情報・知能・精密機器部門講演会講演論文集 2019 巻 ( 0 ) 頁： 2C01 2019年

Separation of large DNA molecules by applying pulsed electric field to size exclusion chromatography-based microchip 査読有り

Azuma, N; Itoh, S; Fukuzawa, K; Zhang, HD

JAPANESE JOURNAL OF APPLIED PHYSICS 57 巻 ( 2 ) 頁： 8 2018年2月

記述言語：日本語掲載種別：研究論文（学術雑誌）出版者・発行元：Japanese Journal of Applied Physics

Through electrophoresis driven by a pulsed electric field, we succeeded in separating large DNA molecules with an electrophoretic microchip based on size exclusion chromatography (SEC), which was proposed in our previous study. The conditions of the pulsed electric field required to achieve the separation were determined by numerical analyses using our originally proposed separation model. From the numerical results, we succeeded in separating large DNA molecules (λ DNA and T4 DNA) within 1600 s, which was approximately half of that achieved under a direct electric field in our previous study. Our SEC-based electrophoresis microchip will be one of the effective tools to meet the growing demand of faster and more convenient separation of large DNA molecules, especially in the field of epidemiological research of infectious diseases.

DOI： 10.7567/JJAP.57.027002

Web of Science

Scopus

Optimization of applied voltages for on-chip concentration of DNA using nanoslit 査読有り

Azuma, N; Itoh, S; Fukuzawa, K; Zhang, HD

JAPANESE JOURNAL OF APPLIED PHYSICS 56 巻 ( 12 ) 頁： 8 2017年12月

記述言語：日本語掲載種別：研究論文（学術雑誌）出版者・発行元：Japanese Journal of Applied Physics

On-chip sample concentration is an effective pretreatment to improve the detection sensitivity of lab-on-a-chip devices for biochemical analysis. In a previous study, we successfully achieved DNA sample concentration using a nanoslit fabricated in the microchannel of a device designed for DNA size separation. The nanoslit was a channel with a depth smaller than the diameter of a random coil-shaped DNA molecule. The concentration was achieved using the entropy trap at the boundary between the microchannel and the nanoslit. DNA molecules migrating toward the nanoslit owing to electrophoresis were trapped in front of the nanoslit and the concentration was enhanced over time. In this study, we successfully maximize the molecular concentration by optimizing the applied voltage for electrophoresis and verifying the effect of temperature. In addition, we propose a model formula that predicts the molecular concentration, the validity of which is confirmed through comparison with experimental results.

DOI： 10.7567/JJAP.56.127001

Web of Science

Scopus

サイズ排除型電気泳動チップにおけるパルス駆動を用いた大分子量 DNA のサイズ分離の高速化

東直輝, 伊藤伸太郎, 福澤健二, 張賀東

IIP情報・知能・精密機器部門講演会講演論文集 2017 巻 ( 0 ) 頁： E-01 - 1 2017年

記述言語：日本語出版者・発行元：一般社団法人日本機械学会

Size separation of large DNA molecules (10 kbp – 1000 kbp) is one of the fundamental procedures for identifying bacterium’s genotypes, which should be fast for quick defection of infectious diseases. In our conventional study, we developed an electrophoresis microchip based on size exclusion chromatography. This separation has an advantage that it does not cause large deformation of DNA, which is important for separation of large DNAs because they can be easily fragmented. We achieved separation of large DNAs (48 kbp + 166 kbp) driven by a direct electric field as electrophoretic condition of DNA. However, it caused long separation time (3000 s) because DNAs migration was slow due to low applied voltage for the separation. In this study, we achieved faster separation of DNAs using a pulsed electric field under which applied voltage can be higher.

DOI： 10.1299/jsmeiip.2017.e-01

CiNii Research

マイクロ流路内に形成したナノスリットによる DNA 試料のオンチップ濃縮

東直輝, 伊藤伸太郎, 福澤健二, 張賀東

マイクロ・ナノ工学シンポジウム 2017.8 巻 ( 0 ) 頁： PN-106 2017年

Separation of large DNA molecules by size exclusion chromatography-based microchip with on-chip concentration structure 査読有り

Naoki Azuma, Shintaro Itoh, Kenji Fukuzawa, Hedong Zhang

Japanese Journal of Applied Physics 55 巻 ( 06GN01 ) 頁： 8 2016年4月

Improvement of resolution in SEC-type DNA separation microchip by on-chip concentration using nano-slit 査読有り

Naoki Azuma, Shintaro Itoh, Yusuke Sugimoto, Kenji Fukuzawa, Hedong Zhang

Proceedings of the JSME-IIP/ASME-ISPS Joint International Conference on Micromechatronics for Information and Precision Equipment 2015 巻 ( MoB-2-4 ) 2015年6月

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