Updated on 2021/10/20

写真a

 
TAKEUCHI Hidenori
 
Organization
Institute for Advanced Research Designated assistant professor
Institute of Transformative Bio-Molecules Designated assistant professor
Title
Designated assistant professor

Degree 1

  1. 博士(理学) ( 2013.3   名古屋大学 ) 

Research Interests 13

  1. chromatin

  2. pollen tube

  3. signaling

  4. cell-to-cell communication

  5. plant reproduction

  6. cysteine-rich peptide

  7. pollen tube growth

  8. pollen tube guidance

  9. cell-cell communication

  10. 植物生殖操作

  11. chrmatin

  12. receptor

  13. molecular evolution

Research Areas 4

  1. Life Science / Molecular biology

  2. Life Science / Plant molecular biology and physiology

  3. Life Science / Plant molecular biology and physiology

  4. Life Science / Molecular biology

Current Research Project and SDGs 1

  1. 種特異的な受精および染色体維持の機構解明と、その制御技術に関する研究

Research History 5

  1. 名古屋大学 高等研究院   特任助教

    2017.4

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    Country:Japan

  2. グレゴール・メンデル研究所(オーストリア)   日本学術振興会 海外特別研究員

    2016.2 - 2018.1

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    Country:Japan

  3. Gregor Mendel Institute of Molecular Plant Biology, Austrian Academy of Sciences   Chromatin Architecture and Function (Frederic Berger Group)   Postdoctoral researcher (JSPS Overseas Research Fellow)

    2016.2 - 2018.1

  4. 名古屋大学大学院 理学研究科   ERATO東山ライブホロニクスプロジェクト   研究員

    2013.4 - 2016.1

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    Country:Japan

  5. 名古屋大学大学院 理学研究科   日本学術振興会特別研究員 (DC1)

    2010.4 - 2013.3

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    Country:Japan

Education 3

  1. Nagoya University   Graduate School, Division of Natural Science

    - 2013.3

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    Country: Japan

  2. Nagoya University   Graduate School, Division of Natural Science

    - 2010.3

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    Country: Japan

  3. Nagoya University   Faculty of Science

    2004.4 - 2008.3

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    Country: Japan

Professional Memberships 5

  1. 日本植物生理学会

  2. 日本植物学会

  3. International Association of Sexual Plant Reproduction Research (IASPRR)

  4. THE JAPANESE SOCIETY OF PLANT PHYSIOLOGISTS

  5. THE BOTANICAL SOCIETY OF JAPAN

Awards 8

  1. 平成30年度科学技術分野の文部科学大臣表彰 若手科学者賞

    2018.4   文部科学省   種特異的な花粉管誘引を担う雌雄鍵分子の研究

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    Country:Japan

  2. The Young Scientists’ Prize (The Commendation for Science and Technology by the Minister of Education, Culture, Sports, Science and Technology)

    2018.4   Ministry of education science sports and culture of japan   Study on male and female key molecules for species-specific pollen tube attraction

    Hidenori TAKEUCHI

  3. Best Poster Award, 24th International Congress on Sexual Plant Reproduction

    2016.3   International Association of Sexual Plant Reproduction Research   Pollen tube sensing of AtLURE1 attractant peptide by tip-localized receptors

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    Award type:Award from international society, conference, symposium, etc.  Country:United States

  4. Best Poster Award, 24th International Congress on Sexual Plant Reproduction

    2016.3   International Association of Sexual Plant Reproduction Research (IASPRR)   Pollen tube sensing of AtLURE1 attractant peptide by tip-localized receptors

    Hidenori TAKEUCHI

  5. 日本植物学会 若手奨励賞

    2014.9   日本植物学会   シロイヌナズナを用いた花粉管ガイダンスの分子機構の解明

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    Country:Japan

  6. BSJ Young Botanist Prize

    2014.9   the Botanical Society of Japan  

  7. 名古屋大学大学院理学研究科 理学研究科長顕彰

    2010.3   名古屋大学大学院理学研究科   シロイヌナズナにおける花粉管誘引物質の同定

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    Country:Japan

  8. 理学研究科長顕彰

    2010.3   名古屋大学大学院理学研究科   シロイヌナズナにおける花粉管誘引物質の同定

    武内 秀憲

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Papers 21

  1. The role of diverse LURE-type cysteine-rich peptides as signaling molecules in plant reproduction. International journal

    Hidenori Takeuchi

    Peptides   Vol. 142   page: 170572 - 170572   2021.8

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    Language:English   Publishing type:Research paper (scientific journal)  

    In angiosperm sexual reproduction, the male pollen tube undergoes a series of interactions with female tissues. For efficient growth and precise guidance, the pollen tube perceives extracellular ligands. In recent decades, various types of secreted cysteine-rich peptides (CRPs) have been identified as peptide ligands that regulate diverse angiosperm reproduction processes, including pollen tube germination, growth, guidance, and rupture. Notably, in two distant core eudicot plants, multiple LURE-type CRPs were found to be secreted from egg-accompanying synergid cells, and these CRPs act as a cocktail of pollen tube attractants for the final step of pollen tube guidance. LURE-type CRPs have species-preferential activity, even among close relatives, and exhibit remarkably divergent molecular evolution with conserved cysteine frameworks, demonstrating that they play a key role in species recognition in pollen tube guidance. In this review, I focus on "reproductive CRPs," particularly LURE-type CRPs, which underlie common but species-specific mechanisms in angiosperm sexual reproduction, and discuss their action, functional regulation, receptors, and evolution.

    DOI: 10.1016/j.peptides.2021.170572

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  2. Persistent directional growth capability in Arabidopsis thaliana pollen tubes after nuclear elimination from the apex. International journal

    Kazuki Motomura, Hidenori Takeuchi, Michitaka Notaguchi, Haruna Tsuchi, Atsushi Takeda, Tetsu Kinoshita, Tetsuya Higashiyama, Daisuke Maruyama

    Nature communications   Vol. 12 ( 1 ) page: 2331 - 2331   2021.4

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    Language:English   Publishing type:Research paper (scientific journal)  

    During the double fertilization process, pollen tubes deliver two sperm cells to an ovule containing the female gametes. In the pollen tube, the vegetative nucleus and sperm cells move together to the apical region where the vegetative nucleus is thought to play a crucial role in controlling the direction and growth of the pollen tube. Here, we report the generation of pollen tubes in Arabidopsis thaliana whose vegetative nucleus and sperm cells are isolated and sealed by callose plugs in the basal region due to apical transport defects induced by mutations in the WPP domain-interacting tail-anchored proteins (WITs) and sperm cell-specific expression of a dominant mutant of the CALLOSE SYNTHASE 3 protein. Through pollen-tube guidance assays, we show that the physiologically anuclear mutant pollen tubes maintain the ability to grow and enter ovules. Our findings provide insight into the sperm cell delivery mechanism and illustrate the independence of the tip-localized vegetative nucleus from directional growth control of the pollen tube.

    DOI: 10.1038/s41467-021-22661-8

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  3. Polyspermy block in the central cell during double fertilization of Arabidopsis thaliana Reviewed

    Shiori Nagahara, Hidenori Takeuchi, Tetsuya Higashiyama

    Frontiers in plant science   Vol. 11   page: 588700   2021.1

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: doi.org/10.3389/fpls.2020.588700

  4. Functional Analysis of the Plant Chromosomal Passenger Complex. Reviewed International journal

    Shinichiro Komaki, Hidenori Takeuchi, Yuki Hamamura, Maren Heese, Takashi Hashimoto, Arp Schnittger

    Plant physiology   Vol. 183 ( 4 ) page: 1586 - 1599   2020.8

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    Language:English   Publishing type:Research paper (scientific journal)  

    The Aurora B kinase, encoded by the AURORA 3 (AUR3) gene in Arabidopsis (Arabidopsis thaliana), is a key regulator of cell division in all eukaryotes. Aurora B has at least two central functions during cell division; it is essential for the correct, i.e. balanced, segregation of chromosomes in mitosis and meiosis by controlling kinetochore function, and it acts at the division plane, where it is necessary to complete cytokinesis. To accomplish these two spatially distinct functions, Aurora B in animals is guided to its sites of action by Borealin, inner centromere protein (INCENP), and Survivin, which, together with Aurora B, form the chromosome passenger complex (CPC). However, besides Aurora homologs, only a candidate gene with restricted homology to INCENP has been described in Arabidopsis, raising the question of whether a full complement of the CPC exists in plants and how Aurora homologs are targeted subcellularly. Here, we have identified and functionally characterized a Borealin homolog, BOREALIN RELATED (BORR), in Arabidopsis. Together with detailed localization studies including the putative Arabidopsis INCENP homolog, these results support the existence of a CPC in plants.

    DOI: 10.1104/pp.20.00344

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  5. Functional analysis of the plant chromosomal passenger complex Reviewed International coauthorship

    Shinichiro Komaki, Hidenori Takeuchi, Yuki Hamamura, Maren Heese, Takashi Hashimoto, Arp Schnittger

    Plant Physiology   Vol. 183 ( 4 ) page: 1586 - 1599   2020.4

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: doi.org/10.1104/pp.20.00344

  6. AtNOT1 Is a Novel Regulator of Gene Expression during Pollen Development. Reviewed

    Kazuki Motomura, Toshihiro Arae, Haruka Araki-Uramoto, Yuya Suzuki, Hidenori Takeuchi, Takamasa Suzuki, Yasunori Ichihashi, Arisa Shibata, Ken Shirasu, Atsushi Takeda, Tetsuya Higashiyama, Yukako Chiba

    Plant & cell physiology   Vol. 61 ( 4 ) page: 712 - 721   2020.4

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    Development of pollen, the male gametophyte of flowering plants, is tightly controlled by dynamic changes in gene expression. Recent research to clarify the molecular aspects of pollen development has revealed the involvement of several transcription factors in the induction of gene expression. However, limited information is available about the factors involved in the negative regulation of gene expression to eliminate unnecessary transcripts during pollen development. In this study, we revealed that AtNOT1 is an essential protein for proper pollen development and germination capacity. AtNOT1 is a scaffold protein of the AtCCR4-NOT complex, which includes multiple components related to mRNA turnover control in Arabidopsis. Phenotypic analysis using atnot1 heterozygote mutant pollen showed that the mature mutant pollen failed to germinate and also revealed abnormal localization of nuclei and a specific protein at the tricellular pollen stage. Furthermore, transcriptome analysis of atnot1 heterozygote mutant pollen showed that the downregulation of a large number of transcripts, along with the upregulation of specific transcripts required for pollen tube germination by AtNOT1 during late microgametogenesis, is important for proper pollen development and germination. Overall, our findings provide new insights into the negative regulation of gene expression during pollen development, by showing the severely defective phonotype of atnot1 heterozygote mutant pollen.

    DOI: 10.1093/pcp/pcz235

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  7. Polyspermy Block in the Central Cell During Double Fertilization of Arabidopsis thaliana. International journal

    Shiori Nagahara, Hidenori Takeuchi, Tetsuya Higashiyama

    Frontiers in plant science   Vol. 11   page: 588700 - 588700   2020

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    During double fertilization in angiosperms, two male gametes (sperm cells), are released from a pollen tube into the receptive region between two female gametes; the egg cell and the central cell of the ovule. The sperm cells fertilize the egg cell and the central cell in a one-to-one manner to yield a zygote and an endosperm, respectively. The one-to-one distribution of the sperm cells to the two female gametes is strictly regulated, possibly via communication among the four gametes. Polyspermy block is the mechanism by which fertilized female gametes prevent fertilization by a secondary sperm cell, and has been suggested to operate in the egg cell rather than the central cell. However, whether the central cell also has the ability to avoid polyspermy during double fertilization remains unclear. Here, we assessed the one-to-one fertilization mechanism of the central cell by laser irradiation of the female gametes and live cell imaging of the fertilization process in Arabidopsis thaliana. We successfully disrupted an egg cell within the ovules by irradiation using a femtosecond pulse laser. In the egg-disrupted ovules, the central cell predominantly showed single fertilization by one sperm cell, suggesting that neither the egg cell nor its fusion with one sperm cell is necessary for one-to-one fertilization (i.e., monospermy) of the central cell. In addition, using tetraspore mutants possessing multiple sperm cell pairs in one pollen, we demonstrated that normal double fertilization was observed even when excess sperm cells were released into the receptive region between the female gametes. In ovules accepting four sperm cells, the egg cell never fused with more than one sperm cell, whereas half of the central cells fused with more than one sperm cell (i.e., polyspermy) even 1 h later. Our results suggest that the central cell can block polyspermy during double fertilization, although the central cell is more permissive to polyspermy than the egg cell. The potential contribution of polyspermy block by the central cell is discussed in terms of how it is involved in the one-to-one distribution of the sperm cells to two distinct female gametes.

    DOI: 10.3389/fpls.2020.588700

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  8. Structural basis for receptor recognition of pollen tube attraction peptides

    Zhang Xiaoxiao, Liu Weijia, Nagae Takuya T., Takeuchi Hidenori, Zhang Heqiao, Han Zhifu, Higashiyama Tetsuya, Chai Jijie

    NATURE COMMUNICATIONS   Vol. 8   2017.11

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1038/s41467-017-01323-8

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  9. The histone H3 variant H3.3 regulates gene body DNA methylation in Arabidopsis thaliana. Reviewed International journal

    Heike Wollmann, Hume Stroud, Ramesh Yelagandula, Yoshiaki Tarutani, Danhua Jiang, Li Jing, Bhagyshree Jamge, Hidenori Takeuchi, Sarah Holec, Xin Nie, Tetsuji Kakutani, Steven E Jacobsen, Frédéric Berger

    Genome biology   Vol. 18 ( 1 ) page: 94 - 94   2017.5

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:BioMed Central Ltd.  

    BACKGROUND: Gene bodies of vertebrates and flowering plants are occupied by the histone variant H3.3 and DNA methylation. The origin and significance of these profiles remain largely unknown. DNA methylation and H3.3 enrichment profiles over gene bodies are correlated and both have a similar dependence on gene transcription levels. This suggests a mechanistic link between H3.3 and gene body methylation. RESULTS: We engineered an H3.3 knockdown in Arabidopsis thaliana and observed transcription reduction that predominantly affects genes responsive to environmental cues. When H3.3 levels are reduced, gene bodies show a loss of DNA methylation correlated with transcription levels. To study the origin of changes in DNA methylation profiles when H3.3 levels are reduced, we examined genome-wide distributions of several histone H3 marks, H2A.Z, and linker histone H1. We report that in the absence of H3.3, H1 distribution increases in gene bodies in a transcription-dependent manner. CONCLUSIONS: We propose that H3.3 prevents recruitment of H1, inhibiting H1's promotion of chromatin folding that restricts access to DNA methyltransferases responsible for gene body methylation. Thus, gene body methylation is likely shaped by H3.3 dynamics in conjunction with transcriptional activity.

    DOI: 10.1186/s13059-017-1221-3

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  10. Tip-localized receptors control pollen tube growth and LURE sensing in Arabidopsis. Reviewed International journal

    Hidenori Takeuchi, Tetsuya Higashiyama

    Nature   Vol. 531 ( 7593 ) page: 245 - 8   2016.3

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:NATURE PUBLISHING GROUP  

    Directional control of tip-growing cells is essential for proper tissue organization and cell-to-cell communication in animals and plants. In the sexual reproduction of flowering plants, the tip growth of the male gametophyte, the pollen tube, is precisely guided by female cues to achieve fertilization. Several female-secreted peptides have recently been identified as species-specific attractants that directly control the direction of pollen tube growth. However, the method by which pollen tubes precisely and promptly respond to the guidance signal from their own species is unknown. Here we show that tip-localized pollen-specific receptor-like kinase 6 (PRK6) with an extracellular leucine-rich repeat domain is an essential receptor for sensing of the LURE1 attractant peptide in Arabidopsis thaliana under semi-in-vivo conditions, and is important for ovule targeting in the pistil. PRK6 interacted with pollen-expressed ROPGEFs (Rho of plant guanine nucleotide-exchange factors), which are important for pollen tube growth through activation of the signalling switch Rho GTPase ROP1 (refs 7, 8). PRK6 conferred responsiveness to AtLURE1 in pollen tubes of the related species Capsella rubella. Furthermore, our genetic and physiological data suggest that PRK6 signalling through ROPGEFs and sensing of AtLURE1 are achieved in cooperation with the other PRK family receptors, PRK1, PRK3 and PRK8. Notably, the tip-focused PRK6 accumulated asymmetrically towards an external AtLURE1 source before reorientation of pollen tube tip growth. These results demonstrate that PRK6 acts as a key membrane receptor for external AtLURE1 attractants, and recruits the core tip-growth machinery, including ROP signalling proteins. This work provides insights into the orchestration of efficient pollen tube growth and species-specific pollen tube attraction by multiple receptors during male-female communication.

    DOI: 10.1038/nature17413

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  11. Rapid Elimination of the Persistent Synergid through a Cell Fusion Mechanism.

    Cell   Vol. 161 ( 4 ) page: 907-18   2015.5

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    DOI: 10.1016/j.cell.2015.03.018

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  12. Live Imaging and Laser Disruption Reveal the Dynamics and Cell-Cell Communication During Torenia fournieri Female Gametophyte Development. Reviewed

    Daichi Susaki, Hidenori Takeuchi, Hiroki Tsutsui, Daisuke Kurihara, Tetsuya Higashiyama

    Plant & cell physiology   Vol. 56 ( 5 ) page: 1031 - 41   2015.5

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:OXFORD UNIV PRESS  

    The female gametophytes of many flowering plants contain one egg cell, one central cell, two synergid cells and three antipodal cells with respective morphological characteristics and functions. These cells are formed by cellularization of a multinuclear female gametophyte. However, the dynamics and mechanisms of female gametophyte development remain largely unknown due to the lack of a system to visualize directly and manipulate female gametophytes in living material. Here, we established an in vitro ovule culture system to examine female gametophyte development in Torenia fournieri, a unique plant species with a protruding female gametophyte. The four-nucleate female gametophyte became eight nucleate by the final (third) mitosis and successively cellularized and matured to attract a pollen tube. The duration of final mitosis was 28 ± 6.5 min, and cellularization was completed in 54 ± 20 min after the end of the third mitosis. Fusion of polar nuclei in the central cell occurred in 13.1 ± 1.1 h, and onset of expression of LURE2, a pollen tube attractant gene, was visualized by a green fluorescent protein reporter 10.7 ± 2.3 h after cellularization. Laser disruption analysis demonstrated that the egg and central cells were required for synergid cells to acquire the pollen tube attraction function. Moreover, aberrant nuclear positioning and down-regulation of LURE2 were observed in one of the two synergid cells after disrupting an immature egg cell, suggesting that cell specification was affected. Our system provides insights into the precise dynamics and mechanisms of female gametophyte development in T. fournieri.

    DOI: 10.1093/pcp/pcv031

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  13. Generation of a homozygous fertilization-defective gcs1 mutant by heat-inducible removal of a rescue gene. Reviewed International journal

    Shiori Nagahara, Hidenori Takeuchi, Tetsuya Higashiyama

    Plant reproduction   Vol. 28 ( 1 ) page: 33 - 46   2015.3

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:SPRINGER  

    Key message: New gametic homozygous mutants. In angiosperms, a haploid male gamete (sperm cell) fuses with a haploid female gamete (egg cell) during fertilization to form a zygote carrying paternally and maternally derived chromosomes. Several fertilization-defective mutants in Arabidopsis thaliana, including a generative cell-specific 1 (gcs1)/hapless 2 mutant, the sperm cells of which are unable to fuse with female gametes, can only be maintained as heterozygous lines due to the infertile male or female gametes. Here, we report successful generation of a gcs1 homozygous mutant by heat-inducible removal of the GCS1 transgene. Using the gcs1 homozygous mutant as male, the defect in gamete fusion was observed with great frequency; in our direct observation by semi-in vivo fertilization assay using ovules, 100 % of discharged sperm cells in culture failed to show gamete fusion. More than 70 % of ovules in the pistil received a second pollen tube as attempted fertilization recovery. Moreover, gcs1 mutant sperm cells could fertilize female gametes at a low frequency in the pistil. This strategy to generate homozygous fertilization-defective mutants will facilitate novel approaches in plant reproduction research.

    DOI: 10.1007/s00497-015-0256-4

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  14. The mechanism and key molecules involved in pollen tube guidance. Reviewed International journal

    Tetsuya Higashiyama, Hidenori Takeuchi

    Annual review of plant biology   Vol. 66   page: 393 - 413   2015

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:ANNUAL REVIEWS  

    During sexual reproduction of flowering plants, pollen tube guidance by pistil tissue is critical for the delivery of nonmotile sperm cells to female gametes. Multistep controls of pollen tube guidance can be divided into two phases: preovular guidance and ovular guidance. During preovular guidance, various female molecules, including stimulants for pollen germination and pollen tube growth, are provided to support tube growth toward the ovary, where the ovules are located. After entering the ovary, pollen tubes receive directional cues from their respective target ovules, including attractant peptides for precise, species-preferential attraction. Successful pollen tube guidance in the pistil requires not only nutritional and directional controls but also competency controls to make pollen tubes responsive to guidance cues, regulation to terminate growth once a pollen tube arrives at the target, and strategies to stop ovular attraction depending on the fertilization of female gametes.

    DOI: 10.1146/annurev-arplant-043014-115635

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  15. Live imaging of calcium spikes during double fertilization in Arabidopsis. Reviewed International journal

    Yuki Hamamura, Moe Nishimaki, Hidenori Takeuchi, Anja Geitmann, Daisuke Kurihara, Tetsuya Higashiyama

    Nature communications   Vol. 5   page: 4722 - 4722   2014.8

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:NATURE PUBLISHING GROUP  

    Ca(2+) waves and oscillation are key signalling elements during the fertilization process of animals, and are involved, for example, in egg activation. In the unique double fertilization process in flowering plants, both the egg cell and the neighbouring central cell fuse with a sperm cell each. Here we succeeded in imaging cytosolic Ca(2+) in these two cells, and in the two synergid cells that accompany the gametes during semi-in vivo double fertilization. Following pollen tube discharge and plasmogamy, the egg and central cells displayed transient Ca(2+) spikes, but not oscillations. Only the events in the egg cell correlated with the plasmogamy. In contrast, the synergid cells displayed Ca(2+) oscillations on pollen tube arrival. The two synergid cells showed distinct Ca(2+) dynamics depending on their respective roles in tube reception. These Ca(2+) dynamics in the female gametophyte seem to represent highly specific signatures that coordinate successful double fertilization in the flowering plants.

    DOI: 10.1038/ncomms5722

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  16. Independent control by each female gamete prevents the attraction of multiple pollen tubes. Reviewed International journal

    Daisuke Maruyama, Yuki Hamamura, Hidenori Takeuchi, Daichi Susaki, Moe Nishimaki, Daisuke Kurihara, Ryushiro D Kasahara, Tetsuya Higashiyama

    Developmental cell   Vol. 25 ( 3 ) page: 317 - 23   2013.5

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:CELL PRESS  

    In flowering plants, double fertilization is normally accomplished by the first pollen tube, with the fertilized ovule subsequently inhibiting the attraction of a second pollen tube. However, the mechanism of second-pollen-tube avoidance remains unknown. We discovered that failure to fertilize either the egg cell or the central cell compromised second-pollen-tube avoidance in Arabidopsis thaliana. A similar disturbance was caused by disrupting the fertilization-independent seed (FIS) class polycomb-repressive complex 2 (FIS-PRC2), a central cell- and endosperm-specific chromatin-modifying complex for gene silencing. Therefore, the two female gametes have evolved their own signaling pathways. Intriguingly, second-pollen-tube attraction induced by half-successful fertilization allowed the ovules to complete double fertilization, producing a genetically distinct embryo and endosperm. We thus propose that each female gamete independently determines second-pollen-tube avoidance to maximize reproductive fitness in flowering plants.

    DOI: 10.1016/j.devcel.2013.03.013

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  17. シロイヌナズナの花粉管誘引物質LUREペプチド群の発見

    武内秀憲, 東山哲也

    植物の生長調節   Vol. 48 ( 1 ) page: 73 - 78   2013

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    Language:Japanese   Publishing type:Research paper (scientific journal)   Publisher:The Japanese Society for Chemical Regulation of Plants  

    Pollen tube guidance is a crucial mechanism for successful fertilization in angiosperms, which precisely controls directional growth of pollen tubes of own species to deliver immotile sperm cells to an embryo sac. Since pollen tubes were observed to grow toward excised pistil tissues on a medium in the late nineteenth century, plant biologists have conducted studies to reveal mechanisms of pollen tube growth and guidance. Recently, pollen tube attractants, LURE peptides, have been identified in a unique dicot, Torenia fournieri. However, universal molecular mechanisms involving species-specific pollen tube attraction are still unclear. In this review, we present identification of pollen tube attractants of Arabidopsis thaliana, AtLURE1 peptides, and discuss universality, species specificity, and expression mechanism of the attractant molecules.

    DOI: 10.18978/jscrp.48.1_73

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    J-GLOBAL

  18. A species-specific cluster of defensin-like genes encodes diffusible pollen tube attractants in Arabidopsis.

    Takeuchi H, Higashiyama T

    PLoS biology   Vol. 10 ( 12 ) page: e1001449   2012

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    DOI: 10.1371/journal.pbio.1001449

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  19. Attraction of tip-growing pollen tubes by the female gametophyte. Reviewed International journal

    Hidenori Takeuchi, Tetsuya Higashiyama

    Current opinion in plant biology   Vol. 14 ( 5 ) page: 614 - 21   2011.10

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:CURRENT BIOLOGY LTD  

    Pollen tube guidance is the mechanism whereby the direction of pollen tube growth is controlled by female cells of the pistil. Some key genes and molecules have recently been identified as being involved in pollen tube guidance. In this review article, we discuss the molecular basis of pollen tube guidance, especially in Arabidopsis thaliana, by summarizing recent progress in various plant species. Attractant molecules and receptors for gametophytic pollen tube guidance are the focus of this article.

    DOI: 10.1016/j.pbi.2011.07.010

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  20. Defensin-like polypeptide LUREs are pollen tube attractants secreted from synergid cells. Reviewed International journal

    Satohiro Okuda, Hiroki Tsutsui, Keiko Shiina, Stefanie Sprunck, Hidenori Takeuchi, Ryoko Yui, Ryushiro D Kasahara, Yuki Hamamura, Akane Mizukami, Daichi Susaki, Nao Kawano, Takashi Sakakibara, Shoko Namiki, Kie Itoh, Kurataka Otsuka, Motomichi Matsuzaki, Hisayoshi Nozaki, Tsuneyoshi Kuroiwa, Akihiko Nakano, Masahiro M Kanaoka, Thomas Dresselhaus, Narie Sasaki, Tetsuya Higashiyama

    Nature   Vol. 458 ( 7236 ) page: 357 - 61   2009.3

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:NATURE PUBLISHING GROUP  

    For more than 140 years, pollen tube guidance in flowering plants has been thought to be mediated by chemoattractants derived from target ovules. However, there has been no convincing evidence of any particular molecule being the true attractant that actually controls the navigation of pollen tubes towards ovules. Emerging data indicate that two synergid cells on the side of the egg cell emit a diffusible, species-specific signal to attract the pollen tube at the last step of pollen tube guidance. Here we report that secreted, cysteine-rich polypeptides (CRPs) in a subgroup of defensin-like proteins are attractants derived from the synergid cells. We isolated synergid cells of Torenia fournieri, a unique plant with a protruding embryo sac, to identify transcripts encoding secreted proteins as candidate molecules for the chemoattractant(s). We found two CRPs, abundantly and predominantly expressed in the synergid cell, which are secreted to the surface of the egg apparatus. Moreover, they showed activity in vitro to attract competent pollen tubes of their own species and were named as LUREs. Injection of morpholino antisense oligomers against the LUREs impaired pollen tube attraction, supporting the finding that LUREs are the attractants derived from the synergid cells of T. fournieri.

    DOI: 10.1038/nature07882

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    PubMed

  21. 花粉管誘引物質ルアーの発見

    東山哲也, 奥田哲弘, 筒井大貴, 椎名恵子, 武内秀憲, 金岡雅浩, 佐々木成江

    化学と生物   Vol. 47 ( 9 ) page: 617 - 623   2009

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    Language:Japanese   Publishing type:Research paper (scientific journal)   Publisher:Japan Society for Bioscience, Biotechnology, and Agrochemistry  

    DOI: 10.1271/kagakutoseibutsu.47.617

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MISC 48

  1. Morphology, dynamics, and function of unique membrane surrounding sperm plasma membrane

    丸山大輔, 泉理恵, 武内秀憲, 東山哲也, 東山哲也, 木下哲, 元村一基

    日本植物生理学会年会(Web)   Vol. 61st   2020

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  2. PRKファミリー受容体による花粉管の伸長・誘引制御の分子機構の解析

    井本美紀, 東山哲也, 東山哲也, 東山哲也, 武内秀憲

    日本植物学会大会研究発表記録   Vol. 83rd   2019

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  3. 助細胞特異的転写因子MYB98に制御される新規誘引物質の探索

    長江拓也, 武内秀憲, 須崎大地, 東山哲也, 東山哲也

    日本植物学会大会研究発表記録   Vol. 83rd   2019

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  4. 植物CENH3の認識とセントロメア積み込みの分子機構の解析

    武内秀憲, 永原史織, 永原史織, 東山哲也, 東山哲也, 東山哲也, BERGER Frederic

    日本植物学会大会研究発表記録   Vol. 83rd   2019

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  5. Searching for factors involved in de novo deposition of the centromeric histone H3 variant after fertilization of Arabidopsis thaliana

    Hidenori Takeuchi, Tetsuya Higashiyama, Frederic Berger

    25th International Congress on Sexual Plant Reproduction     2018.6

  6. 先端をいく花粉管受容体による極性制御機構の解析

    武内秀憲, 武内秀憲, 井本美紀, 長江拓也, 東山哲也, 東山哲也

    日本植物学会大会研究発表記録   Vol. 82nd   page: 121   2018

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  7. 花粉管の伸長および誘引におけるPRK6受容体のシグナル伝達機構の解析

    井本美紀, 東山哲也, 東山哲也, 武内秀憲, 武内秀憲

    日本植物学会大会研究発表記録   Vol. 82nd   page: 151   2018

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  8. 異種と同種を見分ける花粉管誘引における認証機構

    長江拓也, 武内秀憲, 武内秀憲, SRIVASTAVA Ashutosh, TAMA Florence, 東山哲也, 東山哲也

    日本植物学会大会研究発表記録   Vol. 82nd   page: 226   2018

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  9. Species-specific deposition mechanism for centromeric histone H3 variants in land plants

    Hidenori Takeuchi, Frederic Berger

    EMBO Workshop, Histone variants: Molecular functions in health and disease     2017.9

  10. 花粉管誘引物質LUREとその受容体の相互作用に基づく種認証機構の解析

    長江拓也, 武内秀憲, 武内秀憲, 東山哲也, 東山哲也

    日本植物学会大会研究発表記録   Vol. 81st   page: 177   2017

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  11. シロイヌナズナにおいて花粉管の先端に局在する受容体が花粉管の伸長および花粉管誘引ペプチドの感知を制御する

    武内 秀憲

    ライフサイエンス新着論文レビュー     2016.4

  12. Pollen tube sensing of AtLURE1 attractant peptide by tip-localized receptors

    Hidenori Takeuchi, Tetsuya Higashiyama

    24th International Congress on Sexual Plant Reproduction     2016.3

  13. 細胞融合による迅速な残存助細胞排除機構

    丸山大輔, 丸山大輔, 丸山大輔, VOELZ Ronny, 武内秀憲, 森稔幸, 井川智子, 栗原大輔, 河島友和, 植田美那子, 伊藤正樹, 梅田正明, 西川周一, GROSS-HARDT Rita, 東山哲也, 東山哲也, 東山哲也

    Plant Morphology   Vol. 28 ( 1 )   2016

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  14. Tip-localized receptor kinase controls directional growth of pollen tubes toward attractant LURE peptide

    Hidenori Takeuchi, Tetsuya Higashiyama

    Plant receptor kinase: from molecules to environment     2015.2

  15. 受精異常突然変異体gcs1のホモ接合変異体の作出と生理学的解析

    永原史織, 武内秀憲, 東山哲也

    日本植物生理学会年会要旨集   Vol. 56th   page: 251   2015

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  16. 花粉管誘引停止を引き起こす助細胞胚乳融合の解析

    丸山大輔, 丸山大輔, 武内秀憲, 森稔幸, 井川智子, 河島友和, BERGER Frederic, 東山哲也, 東山哲也

    日本生化学会大会(Web)   Vol. 88th   page: 4W20-6 (WEB ONLY)   2015

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  17. 花粉管誘引ペプチドLUREへの応答を担う受容体キナーゼファミリーの解析

    武内秀憲, 武内秀憲, 東山哲也, 東山哲也, 東山哲也

    日本植物学会大会研究発表記録   Vol. 79th   page: 156   2015

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  18. 花粉管誘引ペプチドLUREの受容とシグナル伝達を担う受容体キナーゼの解析

    武内秀憲, 東山哲也

    日本植物生理学会年会要旨集   Vol. 56th   page: 205   2015

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  19. 細胞融合による急速な助細胞排除機構の解析

    丸山大輔, 丸山大輔, VOLZ Ronny, 武内秀憲, 森稔幸, 井川智子, 河島友和, 西川周一, GROSS‐HARDT Rita, 東山哲也, 東山哲也, 東山哲也

    日本細胞生物学会大会要旨集   Vol. 67th   page: 132   2015

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  20. 新奇の植物細胞融合による花粉管誘引停止機構の発見

    丸山大輔, VOELZ Ronny, 武内秀憲, 森稔幸, 河島友和, HARDT Rita Gross, 東山哲也, 東山哲也, 東山哲也

    日本植物学会大会研究発表記録   Vol. 79th   page: 89   2015

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  21. Molecular mechanism controlling pollen tube attraction toward AtLURE1 peptide

    Hidenori Takeuchi, Tetsuya Higashiyama

    23rd International Congress on Sexual Plant Reproduction     2014.7

  22. シロイヌナズナを用いた花粉管ガイダンスの分子機構の解明

    武内秀憲, 武内秀憲

    日本植物学会大会研究発表記録   Vol. 78th   2014

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  23. 雌性配偶体のライブイメージングと構成細胞の機能獲得における細胞間相互作用の解析

    須崎大地, 武内秀憲, 筒井大貴, 東山哲也

    日本植物学会大会研究発表記録   Vol. 78th   page: 165   2014

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  24. 花粉管誘引ペプチドAtLURE1の受容とシグナル伝達に関わる受容体様キナーゼの同定

    武内秀憲, 東山哲也

    日本植物生理学会年会要旨集   Vol. 55th   page: 213   2014

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  25. レーザー細胞破壊とライブイメージングによる重複受精機構の解析

    永原史織, 浜村有希, 武内秀憲, 東山哲也

    日本植物生理学会年会要旨集   Vol. 55th   page: 336   2014

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  26. シロイヌナズナ生体内における花粉管ガイダンスの時空間的解析

    武内秀憲, 東山哲也

    日本植物学会大会研究発表記録   Vol. 78th   page: 248   2014

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  27. Analysis of directional pollen tube growth controlled by LURE peptides in Arabidopsis thaliana

    Hidenori Takeuchi, Tetsuya Higashiyama

    Cold Spring Harbor Asia: Plant Cell and Developmental Biology     2013.6

  28. シロイヌナズナ花粉管誘引ペプチドLUREによる花粉管伸長方向の制御機構の解析

    武内秀憲, 浜村有希, 西巻萌, 東山哲也

    日本植物生理学会年会要旨集   Vol. 54th   page: 310   2013

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  29. 顕微細胞操作による重複受精時の精細胞分配機構の解析

    永原史織, 須崎大地, 武内秀憲, 浜村有希, 東山哲也

    日本植物学会大会研究発表記録   Vol. 77th   page: 247   2013

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  30. 雌性配偶体の経時観察と構成細胞の機能獲得における細胞間相互作用の解析

    須崎大地, 武内秀憲, 椎名恵子, 東山哲也

    日本植物生理学会年会要旨集   Vol. 54th   page: 317   2013

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  31. 胚乳との細胞融合による急速な助細胞排除機構の発見

    丸山大輔, 武内秀憲, 井川智子, 西川周一, 森稔幸, 東山哲也

    日本分子生物学会年会プログラム・要旨集(Web)   Vol. 36th   page: 2P-0669 (WEB ONLY)   2013

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  32. 光細胞操作を用いたシロイヌナズナ重複受精における雌雄配偶子間コミュニケーションの解析

    永原史織, 須崎大地, 武内秀憲, 浜村有希, 東山哲也

    日本植物生理学会年会要旨集   Vol. 54th   page: 189   2013

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  33. 光細胞操作によるシロイヌナズナ雌雄生殖細胞機能の解析

    永原史織, 須崎大地, 武内秀憲, 浜村有希, 東山哲也, 東山哲也

    Plant Morphol   Vol. 25 ( 1 ) page: 114   2013

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  34. ライブイメージングと細胞除去による雌性配偶体構成細胞の機能獲得における細胞間相互作用の解析

    須崎大地, 武内秀憲, 筒井大貴, 椎名恵子, 新田英之, 東山哲也

    日本植物学会大会研究発表記録   Vol. 77th   page: 247   2013

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  35. Functional and evolutionary characterization of attractant peptides in Arabidopsis thaliana

    Hidenori Takeuchi, Tetsuya Higashiyama

    22nd International Congress on Sexual Plant Reproduction     2012.2

  36. シロイヌナズナLUREのトレニアへの遺伝子導入による生殖隔離障壁の打破

    武内秀憲, 東山哲也

    日本植物学会大会研究発表記録   Vol. 76th   page: 136   2012

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  37. シロイヌナズナ花粉管誘引シグナルのin vivoでの時空間的変化

    武内秀憲, 丸山大輔, 浜村有希, 東山哲也

    日本植物生理学会年会要旨集   Vol. 53rd   page: 138   2012

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  38. レーザー細胞操作によるシロイヌナズナ重複受精機構の解析

    永原史織, 須崎大地, 武内秀憲, 浜村有希, 東山哲也

    日本植物学会大会研究発表記録   Vol. 76th   page: 136   2012

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  39. シロイヌナズナ花粉管誘引物質群の種特異的進化

    武内秀憲, 東山哲也

    Plant Morphol   Vol. 24 ( 1 ) page: 124   2012

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  40. Identification of pollen tube attractants in Arabidopsis thaliana

    Hidenori Takeuchi, Tetsuya Higashiyama

    Plant Biology 2011     2011.8

  41. シロイヌナズナを用いた花粉管誘引シグナルの解析

    武内秀憲, 東山哲也

    Plant Morphol   Vol. 23 ( 1 ) page: 95   2011

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  42. 重複遺伝子群AtLURE1による花粉管誘引メカニズムの解明

    武内秀憲, 東山哲也

    日本植物生理学会年会要旨集   Vol. 52nd   page: 116   2011

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    DOI: 10.14841/jspp.2011.0.0024.0

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  43. 花粉管誘引物質AtLURE1ペプチド群の機能と分子進化

    武内秀憲, 東山哲也

    日本植物学会大会研究発表記録   Vol. 75th   page: 164   2011

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  44. シロイヌナズナにおける花粉管誘引物質の同定とその機能

    武内秀憲, 東山哲也

    日本植物生理学会年会要旨集   Vol. 51st   page: 166   2010

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    DOI: 10.14841/jspp.2010.0.0261.0

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  45. シロイヌナズナ花粉管誘引物質の発見

    武内秀憲, 東山哲也

    日本植物学会大会研究発表記録   Vol. 74th   page: 244   2010

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  46. シロイヌナズナにおける花粉管ガイダンスの最終段階ではたらく誘引物質の同定

    武内秀憲, 東山哲也

    日本植物学会大会研究発表記録   Vol. 73rd   page: 109   2009

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  47. シロイヌナズナにおける花粉管ガイダンスの最終段階ではたらく誘引物質の解析

    武内秀憲, 東山哲也

    日本植物生理学会年会要旨集   Vol. 50th   page: 137   2009

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    DOI: 10.14841/jspp.2009.0.0112.0

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  48. 花粉管ガイダンス分子と生殖隔離

    東山哲也, 武内秀憲, 奥田哲弘, 河野直, 金岡雅浩

    日本分子生物学会年会講演要旨集   Vol. 32nd ( Vol.3 ) page: 21   2009

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Presentations 14

  1. 花粉管受容体ファミリーによる極性成長の協調的制御

    井本美紀, 長江拓也, 内木希美, 東山哲也, 武内秀憲

    日本植物学会第84回大会  2020.9.19  日本植物学会

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    Event date: 2020.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:名古屋・オンライン開催  

  2. シロイヌナズナ初期胚発生におけるクロマチン動態のライブイメージング解析

    永原史織,木全祐資,植田美那子,東山哲也,武内秀憲

    第61回日本植物生理学会年会 

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    Event date: 2020.3

    Language:Japanese  

    Country:Japan  

  3. 植物CENH3の認識とセントロメア積み込みの分子機構の解析

    武内秀憲,永原史織,東山哲也,Frederic Berger

    日本植物学会第83回大会 

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    Event date: 2019.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  4. PRKファミリー受容体による花粉管の伸長・誘引制御の分子機構の解析

    井本美紀,東山哲也,武内秀憲

    日本植物学会第83回大会 

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    Event date: 2019.9

    Language:Japanese   Presentation type:Poster presentation  

    Country:Japan  

  5. Molecular recognition mechanism of species-specific centromeric histone H3 variants in plants

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    Event date: 2019.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  6. 先端をいく花粉管受容体による極性制御機構の解析

    武内秀憲,井本美紀,長江拓也,東山哲也

    日本植物学会第82回大会 

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    Event date: 2018.9

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Country:Japan  

  7. 異種と同種を見分ける花粉管誘引における認証機構

    長江拓也, 武内秀憲, Ashutosh Srivastava, Florence Tama, 東山哲也

    日本植物学会第82回大会 

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    Event date: 2018.9

    Language:Japanese   Presentation type:Poster presentation  

    Country:Japan  

  8. 花粉管の伸長および誘引におけるPRK6受容体のシグナル伝達機構の解析

    井本美紀,東山哲也,武内秀憲

    日本植物学会第82回大会 

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    Event date: 2018.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  9. Searching for factors involved in de novo deposition of the centromeric histone H3 variant after fertilization of Arabidopsis thaliana International conference

    Hidenori Takeuchi, Tetsuya Higashiyama, Frederic Berger

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    Event date: 2018.6

    Language:English   Presentation type:Poster presentation  

    Country:Japan  

  10. Species recognition in pollen tube attraction International conference

    Takuya Nagae, Hidenori Takeuchi, Ashutosh Srivastava, Florence Tama, Tetsuya Higashiyama

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    Event date: 2018.6

    Language:English   Presentation type:Poster presentation  

    Country:Japan  

  11. Study on deposition mechanism for the centromeric histone H3 variant in land plants

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    Event date: 2018.3

    Language:Japanese   Presentation type:Poster presentation  

    Country:Japan  

  12. Species-specific deposition mechanism for centromeric histone H3 variants in land plants International conference

    Histone variants: Molecular functions in health and disease 

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    Event date: 2017.9

    Language:English   Presentation type:Poster presentation  

    Country:Germany  

  13. Identification of attractant LURE peptides for pollen tubes in Arabidopsis thaliana(<Feature Article>Plant Peptide Signals)

    Takeuchi Hidenori, Higashiyama Tetsuya

    Regulation of Plant Growth & Development 

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    Event date: 2013

    Language:Japanese   Presentation type:Oral presentation (general)  

    Pollen tube guidance is a crucial mechanism for successful fertilization in angiosperms, which precisely controls directional growth of pollen tubes of own species to deliver immotile sperm cells to an embryo sac. Since pollen tubes were observed to grow toward excised pistil tissues on a medium in the late nineteenth century, plant biologists have conducted studies to reveal mechanisms of pollen tube growth and guidance. Recently, pollen tube attractants, LURE peptides, have been identified in a unique dicot, Torenia fournieri. However, universal molecular mechanisms involving species-specific pollen tube attraction are still unclear. In this review, we present identification of pollen tube attractants of Arabidopsis thaliana, AtLURE1 peptides, and discuss universality, species specificity, and expression mechanism of the attractant molecules.

    DOI: 10.18978/jscrp.48.1_73

    CiNii Article

  14. Discovery of Pollen-tube Attractants LUREs

    HIGASHIYAMA Tetsuya, OKUDA Satohiro, TSUTSUI Hiroki, SHIINA Keiko, TAKEUCHI Hidenori, KANAOKA Masahiro M., SASAKI Narie

    KAGAKU TO SEIBUTSU 

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    Event date: 2009.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    DOI: 10.1271/kagakutoseibutsu.47.617

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Research Project for Joint Research, Competitive Funding, etc. 1

  1. 植物染色体の可視化・人為操作法の開発による生殖ダイナミクスの制御と解明

    2017.11 - 2018.11

    住友財団 2017年度基礎科学研究助成 

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    Grant type:Competitive

KAKENHI (Grants-in-Aid for Scientific Research) 11

  1. 花粉管の先端成長を制御するシグナル伝達の分子基盤と調節機構の解析

    2020.4 - 2023.3

    科学研究費補助金 

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    Authorship:Principal investigator 

  2. 花粉管の先端成長を制御するシグナル伝達の分子基盤と調節機構の解析

    Grant number:20K15817  2020.4 - 2023.3

    日本学術振興会  科学研究費助成事業 若手研究  若手研究

    武内 秀憲

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    Authorship:Principal investigator 

    Grant amount:\4290000 ( Direct Cost: \3300000 、 Indirect Cost:\990000 )

    被子植物が受精を行うためには、花粉管が先端成長して胚珠へと到達する必要がある。花粉管の先端成長は、細胞自律的な「伸長」と外部シグナルによる「方向制御」により制御される。我々はこれまでに、花粉管の方向制御に関わる誘引ペプチドLUREとその受容体PRK6を発見した。興味深いことに、PRK6は花粉管の伸長を制御する細胞内シグナル分子を介して、伸長と方向制御の両方を制御することが示唆されている。本研究では、花粉管の細胞内シグナル分子に着目し、誘引ペプチドLURE-受容体PRK6による調節機構を調べることで、花粉管の先端成長を制御して受精に導くための仕組みを明らかにする。

  3. 受精・胚発生過程における染色体維持装置形成による新生幹細胞維持機構の解明

    2018.4 - 2020.3

    科学研究費補助金  新学術領域研究

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    Authorship:Principal investigator 

  4. 花粉管誘引リガンド-受容体シグナル伝達を制御する複合体ダイナミクスの解析

    2018.4 - 2020.3

    科学研究費補助金 

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    Authorship:Principal investigator 

  5. 受精・胚発生過程における染色体維持装置形成による新生幹細胞維持機構の解明

    Grant number:18H04834  2018.4 - 2020.3

    日本学術振興会  科学研究費助成事業 新学術領域研究(研究領域提案型)  新学術領域研究(研究領域提案型)

    武内 秀憲

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    Authorship:Principal investigator 

    Grant amount:\8580000 ( Direct Cost: \6600000 、 Indirect Cost:\1980000 )

    根源的な“幹細胞”とも言える受精卵が動的に分裂・分化し正常な胚発生を進めるためには、受精卵・初期胚において様々なエピゲノム情報が再編成される必要がある。本研究では被子植物シロイヌナズナを用いて(1)CENH3のセントロメア領域への新規積み込み機構の解析、(2)受精・初期胚発生時におけるエピゲノム動態の解析を行い、植物における染色体維持装置形成の仕組みとそれを制御するエピゲノム因子の解析を進めた。
    (1)様々な植物のCENH3の配列を比較することで、双子葉植物であるシロイヌナズナのセントロメアに運ばれるために重要なCENH3のドメインを絞り込み、セントロメア積み込みに重要なアミノ酸も同定した。また、CENH3を認識するヒストンシャペロン等の探索を進め、CENH3と結合し、CENH3機能に関連する可能性のある分子を同定し、受精・胚発生時における表現型を解析した。これらの解析により、植物のCENH3がセントロメア領域に運ばれ、機能するための特徴が明らかとなった。
    (2)受精前の卵細胞と受精後の受精卵・初期胚で動的に変化するようなエピゲノムマークを同定・解析するために、卵細胞と受精卵の核・細胞膜に加えてエピゲノム因子も蛍光標識した植物株を作出した。この時、セントロメアやヘテロクロマチン領域の形成に影響を及ぼすエピゲノム変異体と組み合わせて準備を進めた。
    今後、(1)と(2)の解析を進めるとともに、関連する分子の探索・同定も進める。得られる知見を基盤としながら受精・胚発生時の様子をライブ解析することで、染色体の維持や幹細胞性の新規確立に重要なエピゲノム動態の解明が期待される。

  6. Analysis of the receptor complex controlling pollen tube attractant signaling

    Grant number:18K14729  2018.4 - 2020.3

    Grants-in-Aid for Scientific Research  Grant-in-Aid for Early-Career Scientists

    Takeuchi Hidenori

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    Authorship:Principal investigator  Grant type:Competitive

    Grant amount:\4160000 ( Direct Cost: \3200000 、 Indirect Cost:\960000 )

    Pollen tube growth and attractant sensing are essential for angiosperm reproduction. Here we studied how the pollen tube receptors regulate growth and attractant sensing through cytoplasmic partner molecules as well as external attractants. By investigating possible cytoplasmic interactors with the receptors, we identified multiple sets of molecules involved in pollen tube growth and attractant sensing. In addition, we showed how the multiple receptors control each of efficient growth and attractant sensing of the pollen tube in a cooperative manner.

  7. 植物染色体の可視化・人為操作法の開発による生殖ダイナミクスの制御と解明

    2017.11 - 2018.11

    住友財団  2017年度基礎科学研究助成 

    武内 秀憲

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    Authorship:Principal investigator  Grant type:Competitive

  8. エピゲノム動態の解析による植物生殖細胞の運命決定機構の解明

    2016.2 - 2018.1

    科学研究費補助金  海外特別研究員

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    Authorship:Principal investigator 

  9. エピゲノム動態の解析による植物生殖細胞の運命決定機構の解明

    2016.2 - 2018.1

    日本学術振興会  海外特別研究員 

    武内 秀憲

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    Authorship:Principal investigator  Grant type:Competitive

  10. シロイヌナズナにおける花粉管誘引物質の発見を基盤とした多段階ガイダンス機構の解明

    2010.4 - 2013.3

    科学研究費補助金 

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    Authorship:Principal investigator 

  11. シロイヌナズナにおける花粉管誘引物質の発見を基盤とした多段階ガイダンス機構の解明

    Grant number:10J05834  2010 - 2012

    日本学術振興会  科学研究費助成事業 特別研究員奨励費  特別研究員奨励費

    武内 秀憲

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    本研究は,モデル植物シロイヌナズナにおいて花粉管誘引物質を同定することを足掛かりとして,花粉管誘引物質の雌しべの中での機能,被子植物における普遍性および種特異的な分子進化を明らかにすることを目的とした。これまでに,シロイヌナズナの助細胞で発現するCRP(cysteine-rich peptide)群を花粉管誘引物質の候補として同定した。その中で最も発現量の高かった6遺伝子の重複遺伝子群によってコードされるペプチド群について解析をおこなったところ,それらがシロイヌナズナの花粉管誘引物質であることの証明に成功し,AtLURE1ペプチド群と名付けた。また近縁種であるセイヨウミヤマハタザオのオーソログとの比較や生理実験により,AtLURE1ペプチド群はシロイヌナズナに特異的な重複遺伝子群によってコードされており,シロイヌナズナに選択的な誘引活性を有していることが示唆された。さらに遠縁種であるトレニアにAtLURE1遺伝子の一つを導入する実験を行った結果,種間における花粉管誘引の生殖隔離障壁を打破できることが明らかとなった。これらの結果は,LURE様のペプチドが被子植物において広く用いられており,種特異的な花粉管誘引に関わる生殖隔離の鍵分子であることを示している。候補として同定したその他のCRPに関しても解析を進めるとともに,近縁種セイヨウミヤマハタザオの花粉を用いた種間かけ合わせ実験や雌しべ内での花粉管ガイダンスの時空間的な詳細な観察も行い,同種の花粉管を選択的に誘引するための花粉管ガイダンスの多段階制御の仕組みの解明につながる結果が得られつつある。

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