研究者詳細 - 野間　健太郎

論文 - 野間　健太郎

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Aberrant neuronal hyperactivation causes an age-dependent behavioral decline in Caenorhabditis elegans. 国際誌

Aleogho BM, Mohri M, Jang MS, Tsukada S, Al-Hebri Y, Matsuyama HJ, Tsukada Y, Mori I, Noma K

Proceedings of the National Academy of Sciences of the United States of America 122 巻 ( 1 ) 頁： e2412391122 2025年1月

Orsay virus infection rate declines with age in C. elegans.

Tishadas S, Noma K

microPublication biology 2025 巻 2025年

Dietary E. coli promotes age-dependent chemotaxis decline in C. elegans.

Suryawinata N, Yokosawa R, Tan KHC, Lai AL, Sone R, Mori I, Noma K

Scientific reports 14 巻 ( 1 ) 頁： 5529 2024年3月

Loss of function in rpms-1 does not enhance phenotypes of rpm-1 mutants. 国際誌

Yue Sun, Daniela Gaio, Bokun Xie, Kentaro Noma, Zilu Wu, Yishi Jin

microPublication biology 2024 巻 2024年

記述言語：英語掲載種別：研究論文（学術雑誌）

The C. elegans E3 ubiquitin ligase RPM-1 consists of 3,766 amino acids, with a RING finger domain at the C-terminus that functions to target the DLK-1 kinase for degradation for synapse development and axon termination. rpms-1 ( for rpm-1 short, aka F07B7.12 ) resides 35 kb away from rpm-1 on chromosome V, and is a near-perfect 12 kb duplication of rpm-1 , including the entire promoter region and coding sequences. RPMS-1 consists of 1,964 amino acids and is identical to the N-terminal half of RPM-1 , except the last 40 amino acids. Previous studies showed that transgenic overexpression of the duplicated region of rpm-1 (+) did not rescue synapse defects of rpm-1 loss of function mutants. Here, using CRISPR editing, we generated a double knockout of rpm-1 and rpms-1 . We find that axon and synapse defects in rpm-1rpms-1 double mutants resemble those in rpm-1 single mutants. Expression levels of endogenously tagged DLK-1 protein are increased to a comparable degree in rpm-1 and rpm-1rpms-1 mutants, compared to the control. These data, along with previous transgene expression analysis, support the idea that rpms-1 does not have a major role in RPM-1-mediated cellular processes.

DOI： 10.17912/micropub.biology.001396

PubMed

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Diets modulate the age-dependent decline of associative learning in <i>Caenorhabditis elegans</i>

Tanaka, M; Tsukada, S; Mohri, M; Higurashi, S; Mori, I; Noma, K

ANNALS OF NUTRITION AND METABOLISM 79 巻頁： 997 - 997 2023年8月

Bacterial diet affects the age-dependent decline of associative learning in Caenorhabditis elegans.

Higurashi S, Tsukada S, Aleogho BM, Park JH, Al-Hebri Y, Tanaka M, Nakano S, Mori I, Noma K

eLife 12 巻 2023年5月

Regulation of Gliogenesis by lin-32/Atoh1 in Caenorhabditis elegans

Zhang Albert, Noma Kentaro, Yan Dong

G3-GENES GENOMES GENETICS 10 巻 ( 9 ) 頁： 3271 - 3278 2020年9月

Designer Palmitoylation Motif-Based Self-Localizing Ligand for Sustained Control of Protein Localization in Living Cells and Caenorhabditis elegans

Nakamura Akinobu, Oki Choji, Sawada Shunsuke, Yoshii Tatsuyuki, Kuwata Keiko, Rudd Andrew K., Devaraj Neal K., Noma Kentaro, Tsukiji Shinya

ACS CHEMICAL BIOLOGY 15 巻 ( 4 ) 頁： 837 - 843 2020年4月

Rapid integration of multi-copy transgenes using optogenetic mutagenesis in Caenorhabditis elegans

Kentaro Noma, Yishi Jin

G3: Genes, Genomes, Genetics 8 巻 ( 6 ) 頁： 2091 - 2097 2018年6月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Genetics Society of America

Stably transmitted transgenes are indispensable for labeling cellular components and manipulating cellular functions. In Caenorhabditis elegans, transgenes are generally generated as inheritable multi-copy extrachromosomal arrays, which can be stabilized in the genome through a mutagenesis-mediated integration process. Standard methods to integrate extrachromosomal arrays primarily use protocols involving ultraviolet light plus trimethylpsoralen or gamma- or X-ray irradiation, which are laborious and time-consuming. Here, we describe a one-step integration method, following germline-mutagenesis induced by mini Singlet Oxygen Generator (miniSOG). Upon blue light treatment, miniSOG tagged to histone (Histone-miniSOG) generates reactive oxygen species (ROS) and induces heritable mutations, including DNA double-stranded breaks. We demonstrate that we can bypass the need to first establish extrachromosomal transgenic lines by coupling microinjection of desired plasmids with blue light illumination on Histone-miniSOG worms to obtain integrants in the F3 progeny.We consistently obtained more than one integrant from 12 injected animals in two weeks. This optogenetic approach significantly reduces the amount of time and labor for transgene integration. Moreover, it enables to generate stably expressed transgenes that cause toxicity in animal growth.

DOI： 10.1534/g3.118.200158

Web of Science

Scopus

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Microtubule-dependent ribosome localization in C. elegans neurons

Kentaro Noma, Alexandr Goncharov, Mark Ellisman, Yishi Jin

ELIFE 6 巻 2017年8月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：ELIFE SCIENCES PUBLICATIONS LTD

Subcellular localization of ribosomes defines the capacity for local translation. Methods to visualize ribosomes in live multicellular organisms are desirable for mechanistic investigations of the cell biology of ribosomes. Here, we developed an approach using split GFP for tissue-specific visualization of ribosomes in live Caonorhabditis elegans. Ribosomes are detected as puncta in the axons and synaptic terminals of specific neuron types, correlating with ribosome distribution at the ultrastructural level. We found that axonal ribosomes change localization during neuronal development and after axonal injury. Using genetic screens, we showed that the microtubule cytoskeleton and the JIP3 protein UNC-16 exert distinct effects on localization of axonal and somatic ribosomes. Our data demonstrate the utility of tissue-specific visualization of ribosomes in vivo, and provide insight into the mechanisms of active regulation of ribosome localization in neurons.

DOI： 10.7554/eLife.26376

Web of Science

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Optogenetic Random Mutagenesis Using Histone-miniSOG in C. elegans.

Noma K, Jin Y

Journal of visualized experiments : JoVE ( 117 ) 2016年11月

Palmitoylation controls DLK localization, interactions and activity to ensure effective axonal injury signaling

Holland Sabrina M., Collura Kaitlin M., Ketschek Andrea, Noma Kentaro, Ferguson Toby A., Jin Yishi, Gallo Gianluca, Thomas Gareth M.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 113 巻 ( 3 ) 頁： 763-768 2016年1月

Optogenetic mutagenesis in Caenorhabditis elegans

Noma Kentaro, Jin Yishi

NATURE COMMUNICATIONS 6 巻 2015年12月

Systematic Analyses of rpm-1 Suppressors Reveal Roles for ESS-2 in mRNA Splicing in Caenorhabditis elegans

Noma Kentaro, Goncharov Alexandr, Jin Yishi

GENETICS 198 巻 ( 3 ) 頁： 1101-+ 2014年11月

Expanding views of presynaptic terminals: new findings from Caenorhabditis elegans

Yan Dong, Noma Kentaro, Jin Yishi

CURRENT OPINION IN NEUROBIOLOGY 22 巻 ( 3 ) 頁： 431-437 2012年6月

Triple N-Glycosylation in the Long S5-P Loop Regulates the Activation and Trafficking of the Kv12.2 Potassium Channel

Noma Kentaro, Kimura Kazushi, Minatohara Keiichiro, Nakashima Hisako, Nagao Yasuaki, Mizoguchi Akira, Fujiyoshi Yoshinori

JOURNAL OF BIOLOGICAL CHEMISTRY 284 巻 ( 48 ) 頁： 33139-33150 2009年11月

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