Updated on 2021/10/21

写真a

 
UCHIHASHI Takayuki
 
Organization
Graduate School of Science Division of Material Science Biophysics Professor
Graduate School
Graduate School of Science
Undergraduate School
School of Science Department of Physics
Title
Professor
Profile
1989 - 1993 B.Sc. Department of Physics, Hiroshima University (Supervisor: Prof. Seizo Morita)
1993 - 1995 M.Sc. Department of Physics, Hiroshima University (Supervisor: Prof. Seizo Morita)
1995 - 1997 PhD course, Department of Physics, Hiroshima University (Supervisor: Prof. Seizo Morita)
1997 - 1998 PhD. Department of Electronics, Osaka University (Supervisor: Prof. Seizo Morita)
1998 - 2000 Postdoctoral Research Fellow, Joint Research Center for Atom Technology, Tsukuba, Japan
2000 - 2002 Assistant Professor, Department of Electronic Engineering, Himeji Institute of Technology, Japan
2002 - 2004 Senior Researcher, SFI Nanoscience Laboratory, Trinity College, Dublin, Ireland
2004 - 2006 Assistant Professor, Department of Physics, Kanazawa University, Japan
2006 - 2015 Associate Professor, College of Science and Engineering, Kanazawa University, Japan
2015 - 2017 Professor, College of Science and Engineering, Kanazawa University, Japan
Director, Bio-AFM Frontier Research Center, Kanazawa University, Japan
2016-2017 Visiting Professor, Institute of Advance Energy, Kyoto University
2017 - Professor, Division of Material Science (Phyics), Graduate School of Science, Nagoya University, Nagoya University
2018 - 2019 Visiting Professor, Department of Life and Coordination-Complex Molecular Science, Institute for Molecular Science
2018 - Visiting Professor, Biomolecular Dynamics Observation Group (Collaborative Research Group), Exploratory Research Center on Life and Living Systems (ExCELLS)
External link

Degree 1

  1. 博士(工学) ( 1998.3   大阪大学 ) 

Research Interests 6

  1. Single-Molecule Imaging

  2. Protein

  3. Biophysics

  4. Scanning Probe Microscopy

  5. High-Speed Atomic Force Microscopy

  6. Atomic Force Microscopy

Research Areas 2

  1. Others / Others  / Nanomaterials/Nanobioscience

  2. Life Science / Biophysics

Research History 9

  1. Nagoya University   Division of Material Science(Physics), Graduate School of Science   Professor

    2017.4

      More details

    Country:Japan

  2. Kanazawa University   Institute of Science and Engineering   Professor

    2015.4 - 2017.3

      More details

    Country:Japan

  3. Kanazawa University   Bio-AFM Frontier Research Center, Institute of Science and Engineering   Director

    2015.4 - 2017.3

      More details

    Country:Japan

  4. Kanazawa University   Institute of Science and Engineering   Associate professor

    2008.4 - 2015.3

      More details

    Country:Japan

  5. Kanazawa University   Graduate School of Natural Science & Technology   Assistant Professor

    2006.4 - 2008.3

      More details

    Country:Japan

  6. Kanazawa University   Graduate School of Natural Science & Technology   Assistant Professor

    2004.4 - 2006.3

      More details

    Country:Japan

  7. Trinity College, Dublin   SFI Nanoscience Institute   Senior Researcher

    2002.8 - 2004.3

      More details

    Country:Japan

  8. Himeji Institute of Technology   Department of Electronic Engineering   Assistant Professor

    2000.4 - 2002.7

      More details

    Country:Japan

  9. Joint Research Center for Atom Technology   Postdoctoral Reseacher

    1998.4 - 2000.3

      More details

    Country:Japan

▼display all

Education 3

  1. Osaka University   Graduate School, Division of Engineering

    - 1998

      More details

    Country: Japan

  2. Hiroshima University   Graduate School, Division of Natural Science

    - 1995

      More details

    Country: Japan

  3. Hiroshima University   Faculty of Science

    - 1993

      More details

    Country: Japan

Professional Memberships 6

  1. 日本応用物理学会

  2. 日本生物物理学会

  3. 日本表面真空学会

  4. 日本化学会

  5. 日本物理学会

  6. 日本分子生物学会

▼display all

Committee Memberships 4

  1. 日本生物物理学会    理事  

    2015 - 2018   

  2. 日本生物物理学会 学会誌「生物物理」編集委員会   委員  

    2014 - 2015   

  3. 日本生物物理学会    代議員  

    2014 - 2015   

  4. 日本生物物理学会 分野別専門委員会   委員  

    2011   

Awards 4

  1. IEEE 3M Nano 2019, Best Conference Paper Award

    2019.8   IEEE 3M Nano  

    Takayuki Uchihashi

     More details

    Award type:Award from international society, conference, symposium, etc.  Country:China

  2. 平成25年度 科学技術分野の文部科学大臣表彰 科学技術賞(開発部門)

    2013.4   文部科学省   高速原子間力顕微鏡の開発

    安藤敏夫, 内橋貴之, 古寺哲幸

     More details

    Country:Japan

  3. 平成21年度 ナノプローブテクノロジー賞

    2009.8   日本学術振興会ナノプローブテクノロジー第167委員会   液中AFMの高速化とタンパク質の動的撮影による研究

    内橋貴之

     More details

    Country:Japan

  4. 第29回応用物理学会論文賞

    2007.9   日本応用物理学会   High-Speed Atomic Force Microscopy for Studying the Dynamic Behavior of Protein Molecules at Work

     More details

    Country:Japan

 

Papers 119

  1. Tardigrade Secretory-Abundant Heat-Soluble Protein Has a Flexible beta-Barrel Structure in Solution and Keeps This Structure in Dehydration

    Miyazawa Kazuhisa, Itoh Satoru G., Watanabe Hiroki, Uchihashi Takayuki, Yanaka Saeko, Yagi-Utsumi Maho, Kato Koichi, Arakawa Kazuharu, Okumura Hisashi

    JOURNAL OF PHYSICAL CHEMISTRY B   Vol. 125 ( 32 ) page: 9145 - 9154   2021.8

     More details

    Language:Japanese  

    DOI: 10.1021/acs.jpcb.1c04850

    Web of Science

  2. JRAB/MICAL-L2 undergoes liquid-liquid phase separation to form tubular recycling endosomes

    Sakane Ayuko, Yano Taka-aki, Uchihashi Takayuki, Horikawa Kazuki, Hara Yusuke, Imoto Issei, Kurisu Shusaku, Yamada Hiroshi, Takei Kohji, Sasaki Takuya

    COMMUNICATIONS BIOLOGY   Vol. 4 ( 1 )   2021.5

     More details

  3. Non-close-packed arrangement of soft elastomer microspheres on solid substrates

    Sasaki Yuma, Hiroshige Seina, Takizawa Masaya, Nishizawa Yuichiro, Uchihashi Takayuki, Minato Haruka, Suzuki Daisuke

    RSC ADVANCES   Vol. 11 ( 24 ) page: 14562 - 14567   2021.5

     More details

    Language:Japanese  

    DOI: 10.1039/d1ra02688g

    Web of Science

  4. Dynamic Assembly/Disassembly of Staphylococcus aureus FtsZ Visualized by High-Speed Atomic Force Microscopy

    Fujita Junso, Sugiyama Shogo, Terakado Haruna, Miyazaki Maho, Ozawa Mayuki, Ueda Nanami, Kuroda Natsuko, Tanaka Shun-ichi, Yoshizawa Takuya, Uchihashi Takayuki, Matsumura Hiroyoshi

    INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES   Vol. 22 ( 4 )   2021.2

  5. Nanostructures, Thermoresponsiveness, and Assembly Mechanism of Hydrogel Microspheres during Aqueous Free-Radical Precipitation Polymerization

    Nishizawa Yuichiro, Minato Haruka, Inui Takumi, Uchihashi Takayuki, Suzuki Daisuke

    LANGMUIR   Vol. 37 ( 1 ) page: 151 - 159   2021.1

  6. Structural insights into the mechanism of rhodopsin phosphodiesterase

    Ikuta Tatsuya, Shihoya Wataru, Sugiura Masahiro, Yoshida Kazuho, Watari Masahito, Tokano Takaya, Yamashita Keitaro, Katayama Kota, Tsunoda Satoshi P., Uchihashi Takayuki, Kandori Hideki, Nureki Osamu

    NATURE COMMUNICATIONS   Vol. 11 ( 1 )   2020.11

  7. Novel Babesia bovis exported proteins that modify properties of infected red blood cells

    Hakimi Hassan, Templeton Thomas J., Sakaguchi Miako, Yamagishi Junya, Miyazaki Shinya, Yahata Kazuhide, Uchihashi Takayuki, Kawazu Shin-ichiro, Kaneko Osamu, Asada Masahito

    PLOS PATHOGENS   Vol. 16 ( 10 )   2020.10

  8. Single-molecule level dynamic observation of disassembly of the apo-ferritin cage in solution

    Maity Basudev, Li Zhipeng, Niwase Kento, Ganser Christian, Furuta Tadaomi, Uchihashi Takayuki, Lu Diannan, Ueno Takafumi

    PHYSICAL CHEMISTRY CHEMICAL PHYSICS   Vol. 22 ( 33 ) page: 18562 - 18572   2020.9

  9. Thermoresponsive structural changes of single poly(N-isopropyl acrylamide) hydrogel microspheres under densely packed conditions on a solid substrate

    Minato Haruka, Nishizawa Yuichiro, Uchihashi Takayuki, Suzuki Daisuke

    POLYMER JOURNAL   Vol. 52 ( 9 ) page: 1137 - 1141   2020.9

  10. Convergent evolution of processivity in bacterial and fungal cellulases

    Uchiyama Taku, Uchihashi Takayuki, Nakamura Akihiko, Watanabe Hiroki, Kaneko Satoshi, Samejima Masahiro, Igarashi Kiyohiko

    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA   Vol. 117 ( 33 ) page: 19896 - 19903   2020.8

  11. Direct visualization of the conformational change of FUS/TLS upon binding to promoter-associated non-coding RNA

    Hamad Nesreen, Watanabe Hiroki, Uchihashi Takayuki, Kurokawa Riki, Nagata Takashi, Katahira Masato

    CHEMICAL COMMUNICATIONS   Vol. 56 ( 64 ) page: 9134 - 9137   2020.8

  12. Scanning Probe Microscopy FOREWORD

    Nakajima Ken, Sumitomo Koji, Nakayama Tomonobu, Fukui Ken-ichi, Kageshima Masami, Kawai Shigeki, Komeda Tadahiro, Takahashi Takuji, Uchihashi Takayuki

    JAPANESE JOURNAL OF APPLIED PHYSICS   Vol. 59   2020.8

  13. Assembly Mechanism of a Supramolecular MS-Ring Complex To Initiate Bacterial Flagellar Biogenesis in Vibrio Species

    Terashima Hiroyuki, Hirano Keiichi, Inoue Yuna, Tokano Takaya, Kawamoto Akihiro, Kato Takayuki, Yamaguchi Erika, Namba Keiichi, Uchihashi Takayuki, Kojima Seiji, Homma Michio

    JOURNAL OF BACTERIOLOGY   Vol. 202 ( 16 )   2020.8

  14. Structural Dynamics of a Protein Domain Relevant to the Water-Oxidizing Complex in Photosystem II as Visualized by High-Speed Atomic Force Microscopy

    Tokano Takaya, Kato Yuki, Sugiyama Shogo, Uchihashi Takayuki, Noguchi Takumi

    JOURNAL OF PHYSICAL CHEMISTRY B   Vol. 124 ( 28 ) page: 5847 - 5857   2020.7

  15. Dynamic behavior of an artificial protein needle contacting a membrane observed by high-speed atomic force microscopy

    Ueno Takafumi, Niwase Kento, Tsubokawa Daisho, Kikuchi Kosuke, Takai Natsumi, Furuta Tadaomi, Kawano Ryuji, Uchihashi Takayuki

    NANOSCALE   Vol. 12 ( 15 ) page: 8166 - 8173   2020.4

  16. Dynamics of oligomer and amyloid fibril formation by yeast prion Sup35 observed by high-speed atomic force microscopy

    Konno Hiroki, Watanabe-Nakayama Takahiro, Uchihashi Takayuki, Okuda Momoko, Zhu Liwen, Kodera Noriyuki, Kikuchi Yousuke, Ando Toshio, Taguchi Hideki

    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA   Vol. 117 ( 14 ) page: 7831 - 7836   2020.4

  17. Schizorhodopsins: A family of rhodopsins from Asgard archaea that function as light-driven inward H+ pumps

    Inoue Keiichi, Tsunoda Satoshi P., Singh Manish, Tomida Sahoko, Hososhima Shoko, Konno Masae, Nakamura Ryoko, Watanabe Hiroki, Bulzu Paul-Adrian, Banciu Horia L., Andrei Adrian-Stefan, Uchihashi Takayuki, Ghai Rohit, Beja Oded, Kandori Hideki

    SCIENCE ADVANCES   Vol. 6 ( 15 )   2020.4

  18. Single-molecule imaging analysis reveals the mechanism of a high-catalytic-activity mutant of chitinase A from Serratia marcescens

    Visootsat Akasit, Nakamura Akihiko, Vignon Paul, Watanabe Hiroki, Uchihashi Takayuki, Iino Ryota

    JOURNAL OF BIOLOGICAL CHEMISTRY   Vol. 295 ( 7 ) page: 1915 - 1925   2020.2

  19. Induced-Fit Pathway Accelerated Binding of Agitoxin-2 to A K+ Channel Imaged by HS-AFM

    Sumino Ayumi, Sumikama Takashi, Uchihashi Takayuki, Oiki Shigetoshi

    BIOPHYSICAL JOURNAL   Vol. 118 ( 3 ) page: 236A - 236A   2020.2

     More details

  20. Construction of a Hexameric Hemoprotein Sheet and Direct Observation of Dynamic Processes of Its Formation

    Oohora Koji, Hirayama Shota, Uchihashi Takayuki, Hayashi Takashi

    CHEMISTRY LETTERS   Vol. 49 ( 2 ) page: 186 - 190   2020.2

  21. High-speed near-field fluorescence microscopy combined with high-speed atomic force microscopy for biological studies

    Umakoshi Takayuki, Fukuda Shingo, Iino Ryota, Uchihashi Takayuki, Ando Toshio

    BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS   Vol. 1864 ( 2 )   2020.2

  22. Supramolecular tholos-like architecture constituted by archaeal proteins without functional annotation

    Yagi-Utsumi Maho, Sikdar Arunima, Song Chihong, Park Jimin, Inoue Rintaro, Watanabe Hiroki, Burton-Smith Raymond N., Kozai Toshiya, Suzuki Tatsuya, Kodama Atsuji, Ishii Kentaro, Yagi Hirokazu, Satoh Tadashi, Uchiyama Susumu, Uchihashi Takayuki, Joo Keehyoung, Lee Jooyoung, Sugiyama Masaaki, Murata Kazuyoshi, Kato Koichi

    SCIENTIFIC REPORTS   Vol. 10 ( 1 )   2020.1

  23. Thermoresponsive Micellar Assembly Constructed from a Hexameric Hemoprotein Modified with Poly(N-isopropylacrylamide) toward an Artificial Light-Harvesting System

    Hirayama Shota, Oohora Koji, Uchihashi Takayuki, Hayashi Takashi

    JOURNAL OF THE AMERICAN CHEMICAL SOCIETY   Vol. 142 ( 4 ) page: 1822 - 1831   2020.1

  24. Dynamics of Inter-Molecular Interactions Between Single A beta(42) Oligomeric and Aggregate Species by High-Speed Atomic Force Microscopy (vol 431, pg 2687, 2019)

    Feng Lei, Watanabe Hiroki, Molino Paul, Wallace Gordon G., Phung Son L., Uchihashi Takayuki, Higgins Michael J.

    JOURNAL OF MOLECULAR BIOLOGY   Vol. 432 ( 2 ) page: 633 - 633   2020.1

  25. Rad50 zinc hook functions as a constitutive dimerization module interchangeable with SMC hinge

    Tatebe Hisashi, Lim Chew Theng, Konno Hiroki, Shiozaki Kazuhiro, Shinohara Akira, Uchihashi Takayuki, Furukohri Asako

    NATURE COMMUNICATIONS   Vol. 11 ( 1 )   2020.1

  26. On-Membrane Dynamic Interplay between Anti-GM1 IgG Antibodies and Complement Component C1q

    Yanaka Saeko, Yogo Rina, Watanabe Hiroki, Taniguchi Yuki, Satoh Tadashi, Komura Naoko, Ando Hiromune, Yagi Hirokazu, Yuki Nobuhiro, Uchihashi Takayuki, Kato Koichi

    INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES   Vol. 21 ( 1 )   2020.1

  27. Structural basis of nucleosome assembly by the Abo1 AAA+ ATPase histone chaperone

    Cho Carol, Jang Juwon, Kang Yujin, Watanabe Hiroki, Uchihashi Takayuki, Kim Seung Joong, Kato Koichi, Lee Ja Yil, Song Ji-Joon

    NATURE COMMUNICATIONS   Vol. 10   2019.12

  28. Crystal structure of heliorhodopsin

    Shihoya Wataru, Inoue Keiichi, Singh Manish, Konno Masae, Hososhima Shoko, Yamashita Keitaro, Ikeda Kento, Higuchi Akimitsu, Izume Tamaki, Okazaki Sae, Hashimoto Masanori, Mizutori Ritsu, Tomida Sahoko, Yamauchi Yumeka, Abe-Yoshizumi Rei, Katayama Kota, Tsunoda Satoshi P., Shibata Mikihiro, Furutani Yuji, Pushkarev Alina, Beja Oded, Uchihashi Takayuki, Kandori Hideki, Nureki Osamu

    NATURE   Vol. 574 ( 7776 ) page: 132 - +   2019.10

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1038/s41586-019-1604-6

    Web of Science

  29. Protein uptake into individual hydrogel microspheres visualized by high-speed atomic force microscopy

    Matsui Shusuke, Hosho Kensuke, Minato Haruka, Uchihashi Takayuki, Suzuki Daisuke

    CHEMICAL COMMUNICATIONS   Vol. 55 ( 68 ) page: 10064 - 10067   2019.9

  30. Evaluation of thermoresponsive structural changes in hydrogel microspheres by high-speed atomic force microscopy

    Nishizawa Yuichiro, Matsui Shusuke, Urayama Kenji, Kureha Takuma, Sibayama Mitsuhiro, Uchihashi Takayuki, Suzuki Daisuke

    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY   Vol. 258   2019.8

     More details

  31. The Fab portion of immunoglobulin G contributes to its binding to Fc gamma receptor III

    Yogo Rina, Yamaguchi Yuki, Watanabe Hiroki, Yagi Hirokazu, Satoh Tadashi, Nakanishi Mahito, Onitsuka Masayoshi, Omasa Takeshi, Shimada Mari, Maruno Takahiro, Torisu Tetsuo, Watanabe Shio, Higo Daisuke, Uchihashi Takayuki, Yanaka Saeko, Uchiyama Susumu, Kato Koichi

    SCIENTIFIC REPORTS   Vol. 9   2019.8

  32. Dynamic structural states of ClpB involved in its disaggregation function (vol 9, 2147, 2018)

    Uchihashi Takayuki, Watanabe Yo-hei, Nakazaki Yosuke, Yamasaki Takashi, Watanabe Hiroki, Maruno Takahiro, Ishii Kentaro, Uchiyama Susumu, Song Chihong, Murata Kazuyoshi, Iino Ryota, Ando Toshio

    NATURE COMMUNICATIONS   Vol. 10   2019.7

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1038/s41467-019-11204-x

    Web of Science

  33. Dynamics of Inter-Molecular Interactions Between Single A beta(42) Oligomeric and Aggregate Species by High-Speed Atomic Force Microscopy

    Feng Lei, Watanabe Hiroki, Molino Paul, Wallace Gordon G., Phung Son L., Uchihashi Takayuki, Higgins Michael J.

    JOURNAL OF MOLECULAR BIOLOGY   Vol. 431 ( 15 ) page: 2687 - 2699   2019.7

  34. An Assessment of the Ability of Submicron- and Micron-Size Silicone Oil Droplets in Dropped Prefillable Syringes to Invoke Early- and Late-Stage Immune Responses

    Krayukhina Elena, Yokoyama Masami, Hayashihara Kayoko Kakuhou, Maruno Takahiro, Noda Masanori, Watanabe Hiroki, Uchihashi Takayuki, Uchiyama Susumu

    JOURNAL OF PHARMACEUTICAL SCIENCES   Vol. 108 ( 7 ) page: 2278 - 2287   2019.7

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.xphs.2019.02.002

    Web of Science

  35. High-speed AFM reveals accelerated binding of agitoxin-2 to a K+ channel by induced fit

    Sumino A., Sumikama T., Uchihashi T., Oiki S.

    SCIENCE ADVANCES   Vol. 5 ( 7 )   2019.7

  36. Non-Thermoresponsive Decanano-sized Domains in Thermoresponsive Hydrogel Microspheres Revealed by Temperature-Controlled High-Speed Atomic Force Microscopy

    Nishizawa Yuichiro, Matsui Shusuke, Urayama Kenji, Kureha Takuma, Shibayama Mitsuhiro, Uchihashi Takayuki, Suzuki Daisuke

    ANGEWANDTE CHEMIE-INTERNATIONAL EDITION   Vol. 58 ( 26 ) page: 8809 - 8813   2019.6

  37. Hydrogel Microellipsoids that Form Robust String-Like Assemblies at the Air/Water Interface

    Honda Kenshiro, Sazuka Yuka, Iizuka Kojiro, Matsui Shusuke, Uchihashi Takayuki, Kureha Takuma, Shibayama Mitsuhiro, Watanabe Takumi, Suzuki Daisuke

    ANGEWANDTE CHEMIE-INTERNATIONAL EDITION   Vol. 58 ( 22 ) page: 7294 - 7298   2019.5

  38. Mutational and Combinatorial Control of Self-Assembling and Disassembling of Human Proteasome Subunits

    Sekiguchi Taichiro, Satoh Tadashi, Kurimoto Eiji, Song Chihong, Kozai Toshiya, Watanabe Hiroki, Ishii Kentaro, Yagi Hirokazu, Yanaka Saeko, Uchiyama Susumu, Uchihashi Takayuki, Murata Kazuyoshi, Kato Koichi

    INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES   Vol. 20 ( 9 )   2019.5

  39. Construction of a Quadrangular Tetramer and a Cage-Like Hexamer from Three-Helix Bundle-Linked Fusion Proteins

    Miyamoto Takaaki, Hayashi Yugo, Yoshida Keito, Watanabe Hiroki, Uchihashi Takayuki, Yonezawa Kento, Shimizu Nobutaka, Kamikubo Hironari, Hirota Shun

    ACS SYNTHETIC BIOLOGY   Vol. 8 ( 5 ) page: 1112 - 1120   2019.5

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1021/acssynbio.9b00019

    Web of Science

  40. Inner lumen proteins stabilize doublet microtubules in cilia and flagella

    Owa Mikito, Uchihashi Takayuki, Yanagisawa Haru-aki, Yamano Takashi, Iguchi Hiro, Fukuzawa Hideya, Wakabayashi Ken-ichi, Ando Toshio, Kikkawa Masahide

    NATURE COMMUNICATIONS   Vol. 10   2019.3

  41. A ring-shaped hemoprotein trimer thermodynamically controlled by the supramolecular heme-heme pocket interaction

    Oohora Koji, Kajihara Ryota, Fujimaki Nishiki, Uchihashi Takayuki, Hayashi Takashi

    CHEMICAL COMMUNICATIONS   Vol. 55 ( 11 ) page: 1544-1547   2019.2

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1039/c8cc09314h

    Web of Science

  42. Microtubule self- healing and defect creation investigated by in- line force measurements during high- speed atomic force microscopy imaging

    Ganser Christian, Uchihashi Takayuki

    NANOSCALE   Vol. 11 ( 1 ) page: 125 - 135   2019.1

  43. Single-Unit Imaging of Membrane Protein-Embedded Nanodiscs from Two Oriented Sides by High-Speed Atomic Force Microscopy

    Haruyama Takamitsu, Sugano Yasunori, Kodera Noriyuki, Uchihashi Takayuki, Ando Toshio, Tanaka Yoshiki, Konno Hiroki, Tsukazaki Tomoya

    STRUCTURE   Vol. 27 ( 1 ) page: 152 - +   2019.1

  44. Metastable asymmetrical structure of a shaftless V-1 motor

    Maruyama Shintaro, Suzuki Kano, Imamura Motonori, Sasaki Hikaru, Matsunami Hideyuki, Mizutani Kenji, Saito Yasuko, Imai Fabiana L., Ishizuka-Katsura Yoshiko, Kimura-Someya Tomomi, Shirouzu Mikako, Uchihashi Takayuki, Ando Toshio, Yamato Ichiro, Murata Takeshi

    SCIENCE ADVANCES   Vol. 5 ( 1 )   2019.1

  45. Real-time Nanoscale Visualization of Biological Molecules at Work with High-speed Atomic Force Microscopy

    Uchihashi Takayuki

    2019 IEEE INTERNATIONAL CONFERENCE ON MANIPULATION, MANUFACTURING AND MEASUREMENT ON THE NANOSCALE (IEEE 3M-NANO)     page: 253 - 256   2019

     More details

  46. Development of Wide-area Tip-scanning High-speed Atomic Force Microscopy

    Watanabe Hiroki, Uchihashi Takayuki

    2019 IEEE INTERNATIONAL CONFERENCE ON MANIPULATION, MANUFACTURING AND MEASUREMENT ON THE NANOSCALE (IEEE 3M-NANO)     page: 281 - 285   2019

     More details

  47. Direct Observation and Manipulation of Supramolecular Polymerization by High-Speed Atomic Force Microscopy

    Fukui Tomoya, Uchihashi Takayuki, Sasaki Norihiko, Watanabe Hiroki, Takeuchi Masayuki, Sugiyasu Kazunori

    ANGEWANDTE CHEMIE-INTERNATIONAL EDITION   Vol. 57 ( 47 ) page: 15465-15470   2018.11

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1002/anie.201809165

    Web of Science

  48. Monitoring Thermoresponsive Morphological Changes in Individual Hydrogel Microspheres

    Matsui Shusuke, Nishizawa Yuichiro, Uchihashi Takayuki, Suzuki Daisuke

    ACS OMEGA   Vol. 3 ( 9 ) page: 10836-10842   2018.9

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1021/acsomega.8b01770

    Web of Science

  49. Supramolecular Hemoprotein Assembly with a Periodic Structure Showing Heme-Heme Exciton Coupling

    Oohora Koji, Fujimaki Nishiki, Kajihara Ryota, Watanabe Hiroki, Uchihashi Takayuki, Hayashi Takashi

    JOURNAL OF THE AMERICAN CHEMICAL SOCIETY   Vol. 140 ( 32 ) page: 10145-10148   2018.8

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1021/jacs.8b06690

    Web of Science

  50. Revealing circadian mechanisms of integration and resilience by visualizing clock proteins working in real time

    Mori Tetsuya, Sugiyama Shogo, Byrne Mark, Johnson Carl Hirschie, Uchihashi Takayuki, Ando Toshio

    NATURE COMMUNICATIONS   Vol. 9   2018.8

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1038/s41467-018-05438-4

    Web of Science

  51. Scanning Probe Microscopy FOREWORD

    Takahashi Takuji, Fukui Ken-ichi, Kageshima Masami, Komeda Tadahiro, Nakajima Ken, Nakayama Tomonobu, Sumitomo Koji, Uchihashi Takayuki

    JAPANESE JOURNAL OF APPLIED PHYSICS   Vol. 57 ( 8 )   2018.8

  52. Quantum-dot antibody conjugation visualized at the single-molecule scale with high-speed atomic force microscopy

    Umakoshi Takayuki, Udaka Hikari, Uchihashi Takayuki, Ando Toshio, Suzuki Miho, Fukuda Takeshi

    COLLOIDS AND SURFACES B-BIOINTERFACES   Vol. 167   page: 267-274   2018.7

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.colsurfb.2018.04.015

    Web of Science

  53. Translating MOF chemistry into supramolecular chemistry: soluble coordination nanofibers showing efficient photon upconversion

    Hosoyamada Masanori, Yanai Nobuhiro, Okumura Keisuke, Uchihashi Takayuki, Kimizuka Nobuo

    CHEMICAL COMMUNICATIONS   Vol. 54 ( 50 ) page: 6828-6831   2018.6

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1039/c8cc01594e

    Web of Science

  54. Dynamic structural states of CIpB involved in its disaggregation function

    Uchihashi Takayuki, Watanabe Yo-hei, Nakazaki Yosuke, Yamasaki Takashi, Watanabe Hiroki, Maruno Takahiro, Ishii Kentaro, Uchiyama Susumu, Song Chihong, Murata Kazuyoshi, Iino Ryota, Ando Toshio

    NATURE COMMUNICATIONS   Vol. 9   2018.6

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1038/s41467-018-04587-w

    Web of Science

  55. Sweeping of adsorbed therapeutic protein on prefillable syringes promotes micron aggregate generation. Reviewed

    Maruno T, Watanabe H, Yoneda S, Uchihashi T, Adachi S, Arai K, Sawaguchi T, Uchiyama S

    Journal of pharmaceutical sciences   Vol. 107 ( 6 ) page: 1521 - 1529   2018.6

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.xphs.2018.01.021

    Web of Science

    PubMed

  56. Oligomeric states of microbial rhodopsins determined by high-speed atomic force microscopy and circular dichroic spectroscopy

    Shibata Mikihiro, Inoue Keiichi, Ikeda Kento, Konno Masae, Singh Manish, Kataoka Chihiro, Abe-Yoshizumi Rei, Kandori Hideki, Uchihashi Takayuki

    SCIENTIFIC REPORTS   Vol. 8   2018.5

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1038/s41598-018-26606-y

    Web of Science

  57. Structural properties determining low K+ affinity of the selectivity filter in the TWIK1 K+ channel

    Tsukamoto Hisao, Higashi Masahiro, Motoki Hideyoshi, Watanabe Hiroki, Ganser Christian, Nakajo Koichi, Kubo Yoshihiro, Uchihashi Takayuki, Furutani Yuji

    JOURNAL OF BIOLOGICAL CHEMISTRY   Vol. 293 ( 18 ) page: 6969 - 6984   2018.5

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1074/jbc.RA118.001817

    Web of Science

  58. Construction of a Triangle-Shaped Trimer and a Tetrahedron Using an -Helix-Inserted Circular Permutant of Cytochrome c(555)

    Oda Akiya, Nagao Satoshi, Yamanaka Masaru, Ueda Ikki, Watanabe Hiroki, Uchihashi Takayuki, Shibata Naoki, Higuchi Yoshiki, Hirota Shun

    CHEMISTRY-AN ASIAN JOURNAL   Vol. 13 ( 8 ) page: 964-967   2018.4

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1002/asia.201800252

    Web of Science

  59. Insight into structural remodeling of the FlhA ring responsible for bacterial flagellar type III protein export

    Terahara Naoya, Inoue Yumi, Kodera Noriyuki, Morimoto Yusuke V., Uchihashi Takayuki, Imada Katsumi, Ando Toshio, Namba Keiichi, Minamino Tohru

    SCIENCE ADVANCES   Vol. 4 ( 4 )   2018.4

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1126/sciadv.aao7054

    Web of Science

  60. High-speed AFM reveals detail understanding in adsorption of soft hydrogel microspheres onto solid substrate in aqueous solution

    Matsui Shusuke, Uchihashi Takayuki, Suzuki Daisuke

    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY   Vol. 255   page: .   2018.3

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    Web of Science

  61. Real-time adsorption behavior of hydrogel microspheres onto solid/liquid interface

    Matsui Shusuke, Uchihashi Takayuki, Suzuki Daisuke

    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY   Vol. 255   page: .   2018.3

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    Web of Science

  62. Rate constants, processivity, and productive binding ratio of chitinase A revealed by single-molecule analysis.

    Nakamura A, Tasaki T, Okuni Y, Song C, Murata K, Kozai T, Hara M, Sugimoto H, Suzuki K, Watanabe T, Uchihashi T, Noji H, Iino R

    Physical chemistry chemical physics : PCCP   Vol. 20 ( 5 ) page: 3010 - 3018   2018.2

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1039/c7cp04606e

    Web of Science

    PubMed

  63. Rate constants, processivity, and productive binding ratio of chitinase A revealed by single-molecule analysis (vol 20, pg 3010, 2018)

    Nakamura Akihiko, Tasaki Tomoyuki, Okuni Yasuko, Song Chihong, Murata Kazuyoshi, Kozai Toshiya, Hara Mayu, Sugimoto Hayuki, Suzuki Kazushi, Watanabe Takeshi, Uchihashi Takayuki, Noji Hiroyuki, Iino Ryota

    PHYSICAL CHEMISTRY CHEMICAL PHYSICS   Vol. 20 ( 5 ) page: 3844-3844   2018.2

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1039/c8cp90024h

    Web of Science

  64. Dynamic Observation of Kai Proteins by HS-AFM Reveals a Mechanism of the Robustness in the Cyanobacterial Circadian Oscillator

    Sugiyama Shogo, Mori Tetsya, Byrne Mark, Uchihashi Takayuki, Johnson Carl H., Ando Toshio

    BIOPHYSICAL JOURNAL   Vol. 114 ( 3 ) page: 68A-68A   2018.2

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    Web of Science

  65. Applications of high-speed atomic force microscopy to real-time visualization of dynamic biomolecular processes.

    Uchihashi T, Scheuring S

    Biochimica et biophysica acta   Vol. 1862 ( 2 ) page: 229 - 240   2018.2

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.bbagen.2017.07.010

    Web of Science

    PubMed

  66. Dynamic clustering of dynamin-amphiphysin helices regulates membrane constriction and fission coupled with GTP hydrolysis. Reviewed

    Takeda T, Kozai T, Yang H, Ishikuro D, Seyama K, Kumagai Y, Abe T, Yamada H, Uchihashi T, Ando T, Takei K

    eLife   Vol. 7   2018.1

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.7554/eLife.30246

    Web of Science

    PubMed

  67. Negatively Charged Lipids Are Essential for Functional and Structural Switch of Human 2-Cys Peroxiredoxin II. Reviewed

    Haruyama T, Uchihashi T, Yamada Y, Kodera N, Ando T, Konno H

    Journal of molecular biology     2018.1

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.jmb.2017.12.020

    PubMed

  68. Conversion of functionally undefined homopentameric protein PbaA into a proteasome activator by mutational modification of its C-terminal segment conformation. Reviewed

    Yagi-Utsumi M, Sikdar A, Kozai T, Inoue R, Sugiyama M, Uchihashi T, Yagi H, Satoh T, Kato K

    Protein engineering, design & selection : PEDS   Vol. 31 ( 1 ) page: 29 - 36   2018.1

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1093/protein/gzx066

    Web of Science

    PubMed

  69. Visualization of Protein Dynamics using High-Speed Atomic Force Microscopy and Image Analysis

    Uchihashi Takayuki

    JOURNAL OF COMPUTER CHEMISTRY-JAPAN   Vol. 17 ( 1 ) page: 20-30   2018

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.2477/jccj.2018-0001

    Web of Science

  70. Optimum Substrates for Imaging Biological Molecules with High-Speed Atomic Force Microscopy

    Uchihashi Takayuki, Watanabe Hiroki, Kodera Noriyuki

    NANOSCALE IMAGING: METHODS AND PROTOCOLS   Vol. 1814   page: 159 - 179   2018

  71. Two-step process for disassembly mechanism of proteasome alpha 7 homo-tetradecamer by alpha 6 revealed by high-speed atomic force microscopy

    Kozai Toshiya, Sekiguchi Taichiro, Satoh Tadashi, Yagi Hirokazu, Kato Koichi, Uchihashi Takayuki

    SCIENTIFIC REPORTS   Vol. 7 ( 1 ) page: 15373   2017.11

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1038/s41598-017-15708-8

    Web of Science

    PubMed

  72. Real-space and real-time dynamics of CRISPR-Cas9 visualized by high-speed atomic force microscopy.

    Shibata M, Nishimasu H, Kodera N, Hirano S, Ando T, Uchihashi T, Nureki O

    Nature communications   Vol. 8 ( 1 ) page: 1430   2017.11

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1038/s41467-017-01466-8

    Web of Science

    PubMed

  73. Na<sup>+</sup>-induced structural transition of MotPS for stator assembly of the <i>Bacillus</i> flagellar motor.

    Terahara N, Kodera N, Uchihashi T, Ando T, Namba K, Minamino T

    Science advances   Vol. 3 ( 11 ) page: eaao4119   2017.11

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1126/sciadv.aao4119

    Web of Science

    PubMed

  74. Fast Adsorption of Soft Hydrogel Microspheres on Solid Surfaces in Aqueous Solution.

    Matsui S, Kureha T, Hiroshige S, Shibata M, Uchihashi T, Suzuki D

    Angewandte Chemie (International ed. in English)   Vol. 56 ( 40 ) page: 12146 - 12149   2017.9

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1002/anie.201705808

    Web of Science

    PubMed

  75. Visualisation of a flexible modular structure of the ER folding-sensor enzyme UGGT.

    Satoh T, Song C, Zhu T, Toshimori T, Murata K, Hayashi Y, Kamikubo H, Uchihashi T, Kato K

    Scientific reports   Vol. 7 ( 1 ) page: 12142   2017.9

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1038/s41598-017-12283-w

    Web of Science

    PubMed

  76. Interdomain flip-flop motion visualized in flavocytochrome cellobiose dehydrogenase using high-speed atomic force microscopy during catalysis.

    Harada H, Onoda A, Uchihashi T, Watanabe H, Sunagawa N, Samejima M, Igarashi K, Hayashi T

    Chemical science   Vol. 8 ( 9 ) page: 6561 - 6565   2017.9

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1039/c7sc01672g

    Web of Science

    PubMed

  77. Scanning Probe Microscopy FOREWORD

    Takahashi Takuji, Fukui Ken-ichi, Kageshima Masami, Komeda Tadahiro, Nakajima Ken, Nakayama Tomonobu, Sumitomo Koji, Uchihashi Takayuki

    JAPANESE JOURNAL OF APPLIED PHYSICS   Vol. 56 ( 8 )   2017.8

  78. High-Resolution Imaging of a Single Gliding Protofilament of Tubulins by HS-AFM.

    Keya JJ, Inoue D, Suzuki Y, Kozai T, Ishikuro D, Kodera N, Uchihashi T, Kabir AMR, Endo M, Sada K, Kakugo A

    Scientific reports   Vol. 7 ( 1 ) page: 6166   2017.7

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1038/s41598-017-06249-1

    PubMed

  79. High-Speed Atomic Force Microscopy Reveals Loss of Nuclear Pore Resilience as a Dying Code in Colorectal Cancer Cells.

    Mohamed MS, Kobayashi A, Taoka A, Watanabe-Nakayama T, Kikuchi Y, Hazawa M, Minamoto T, Fukumori Y, Kodera N, Uchihashi T, Ando T, Wong RW

    ACS nano   Vol. 11 ( 6 ) page: 5567-5578   2017.6

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1021/acsnano.7b00906

    PubMed

  80. Oriented Reconstitution of the Full-Length KcsA Potassium Channel in a Lipid Bilayer for AFM Imaging. Reviewed

    Sumino A, Uchihashi T, Oiki S

    The journal of physical chemistry letters   Vol. 8 ( 4 ) page: 785-793   2017.2

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1021/acs.jpclett.6b03058

    PubMed

  81. Nuclear Pore Selective Barrier Dynamics as Revealed by High-Speed Atomic Force Microscopy in Colorectal Cancer Cells.

    Mohamed M. S., Kobayashi A., Taoka A., Watanabe-Nakayama T., Kikuchi Y., Hazawa M., Minamoto T., Fukumori Y., Kodera N., Uchihashi T., Ando T., Wong R.

    MOLECULAR BIOLOGY OF THE CELL   Vol. 28   2017

     More details

    Language:Japanese  

    Web of Science

  82. High-speed atomic force microscopy imaging of live mammalian cells.

    Shibata M, Watanabe H, Uchihashi T, Ando T, Yasuda R

    Biophysics and physicobiology   Vol. 14   page: 127 - 135   2017

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.2142/biophysico.14.0_127

    Web of Science

    PubMed

  83. "Clusterase" model of dynamin-mediated membrane fission.

    Takeda T., Kozai T., Yang H., Kaho S., Yusuke K., Tadashi A., Hiroshi Y., Uchihashi T., Ando T., Takei K.

    MOLECULAR BIOLOGY OF THE CELL   Vol. 28   page: .   2017

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    Web of Science

  84. A natural light-driven inward proton pump. Reviewed

    Inoue K, Ito S, Kato Y, Nomura Y, Shibata M, Uchihashi T, Tsunoda SP, Kandori H

    Nature communications   Vol. 7   page: 13415   2016.11

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1038/ncomms13415

    PubMed

  85. Functional extension of high-speed AFM for wider biological applications. Reviewed

    Uchihashi T, Watanabe H, Fukuda S, Shibata M, Ando T

    Ultramicroscopy   Vol. 160   page: 182-96   2016.1

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.ultramic.2015.10.017

    PubMed

  86. Potential Prepore Trimer Formation by the Bacillus thuringiensis Mosquito-specific Toxin: MOLECULAR INSIGHTS INTO A CRITICAL PREREQUISITE OF MEMBRANE-BOUND MONOMERS. Reviewed

    Sriwimol W, Aroonkesorn A, Sakdee S, Kanchanawarin C, Uchihashi T, Ando T, Angsuthanasombat C

    The Journal of biological chemistry   Vol. 290 ( 34 ) page: 20793-803   2015.8

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1074/jbc.M114.627554

    PubMed

  87. Method of mechanical holding of cantilever chip for tip-scan high-speed atomic force microscope. Reviewed

    Fukuda S, Uchihashi T, Ando T

    The Review of scientific instruments   Vol. 86 ( 6 ) page: 063703   2015.6

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1063/1.4922381

    PubMed

  88. Long-tip high-speed atomic force microscopy for nanometer-scale imaging in live cells. Reviewed

    Shibata M, Uchihashi T, Ando T, Yasuda R

    Scientific reports   Vol. 5   page: 8724   2015.3

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1038/srep08724

    PubMed

  89. Probing structural dynamics of an artificial protein cage using high-speed atomic force microscopy. Reviewed

    Imamura M, Uchihashi T, Ando T, Leifert A, Simon U, Malay AD, Heddle JG

    Nano letters   Vol. 15 ( 2 ) page: 1331-5   2015.2

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1021/nl5045617

    PubMed

  90. Real-time dynamic adsorption processes of cytochrome c on an electrode observed through electrochemical high-speed atomic force microscopy. Reviewed

    Takeda K, Uchihashi T, Watanabe H, Ishida T, Igarashi K, Nakamura N, Ohno H

    PloS one   Vol. 10 ( 2 ) page: e0116685   2015

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1371/journal.pone.0116685

    PubMed

  91. Two-way traffic of glycoside hydrolase family 18 processive chitinases on crystalline chitin. Reviewed

    Igarashi K, Uchihashi T, Uchiyama T, Sugimoto H, Wada M, Suzuki K, Sakuda S, Ando T, Watanabe T, Samejima M

    Nature communications   Vol. 5   page: 3975   2014.6

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1038/ncomms4975

    PubMed

  92. Single-molecule imaging analysis of elementary reaction steps of Trichoderma reesei cellobiohydrolase I (Cel7A) hydrolyzing crystalline cellulose Iα and IIII.

      Vol. 289 ( 20 ) page: 14056-65   2014.5

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1074/jbc.M113.546085

    PubMed

  93. [Direct observation of rotary catalysis of rotorless F1-ATPase with high-speed AFM].

    Uchihashi T, Iino R, Ando T, Noji H

    Seikagaku. The Journal of Japanese Biochemical Society   Vol. 86 ( 2 ) page: 127-36   2014.4

     More details

    Language:Japanese   Publishing type:Research paper (scientific journal)  

    PubMed

  94. Trade-off between processivity and hydrolytic velocity of cellobiohydrolases at the surface of crystalline cellulose.

    Nakamura A, Watanabe H, Ishida T, Uchihashi T, Wada M, Ando T, Igarashi K, Samejima M

    Journal of the American Chemical Society   Vol. 136 ( 12 ) page: 4584-92   2014.3

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1021/ja4119994

    PubMed

  95. Filming biomolecular processes by high-speed atomic force microscopy.

    Ando T, Uchihashi T, Scheuring S

    Chemical reviews   Vol. 114 ( 6 ) page: 3120-88   2014.3

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1021/cr4003837

    PubMed

  96. Role of trimer-trimer interaction of bacteriorhodopsin studied by optical spectroscopy and high-speed atomic force microscopy.

    Yamashita H, Inoue K, Shibata M, Uchihashi T, Sasaki J, Kandori H, Ando T

    Journal of structural biology   Vol. 184 ( 1 ) page: 2-11   2013.10

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.jsb.2013.02.011

    PubMed

  97. Real-time visualization of assembling of a sphingomyelin-specific toxin on planar lipid membranes.

    Yilmaz N, Yamada T, Greimel P, Uchihashi T, Ando T, Kobayashi T

    Biophysical journal   Vol. 105 ( 6 ) page: 1397-405   2013.9

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.bpj.2013.07.052

    PubMed

  98. High-speed atomic force microscope combined with single-molecule fluorescence microscope.

    Fukuda S, Uchihashi T, Iino R, Okazaki Y, Yoshida M, Igarashi K, Ando T

    The Review of scientific instruments   Vol. 84 ( 7 ) page: 073706   2013.7

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1063/1.4813280

    PubMed

  99. Wide-area scanner for high-speed atomic force microscopy.

    Watanabe H, Uchihashi T, Kobashi T, Shibata M, Nishiyama J, Yasuda R, Ando T

    The Review of scientific instruments   Vol. 84 ( 5 ) page: 053702   2013.5

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1063/1.4803449

    PubMed

  100. High-speed AFM and applications to biomolecular systems.

    Ando T, Uchihashi T, Kodera N

    Annual review of biophysics   Vol. 42   page: 393-414   2013

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1146/annurev-biophys-083012-130324

    PubMed

  101. Single-molecule imaging on living bacterial cell surface by high-speed AFM.

    Yamashita H, Taoka A, Uchihashi T, Asano T, Ando T, Fukumori Y

    Journal of molecular biology   Vol. 422 ( 2 ) page: 300-9   2012.9

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.jmb.2012.05.018

    PubMed

  102. Guide to video recording of structure dynamics and dynamic processes of proteins by high-speed atomic force microscopy.

    Uchihashi T, Kodera N, Ando T

    Nature protocols   Vol. 7 ( 6 ) page: 1193-206   2012.5

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1038/nprot.2012.047

    PubMed

  103. Direct Visualization of Cellobiohydrolase on Crystalline Cellulose using High-Speed Atomic Force Microscopy Reviewed

    Uchihashi Takayuki, Igarashi Kiyohiko, Koivula Anu, Wada Masahisa, Penttil Merja, Samejima Masahiro, Ando Toshio

    BIOPHYSICAL JOURNAL   Vol. 102 ( 3 ) page: 585A-586A   2012.1

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    Web of Science

  104. Visualization of cellobiohydrolase I from Trichoderma reesei moving on crystalline cellulose using high-speed atomic force microscopy.

    Methods in enzymology   Vol. 510   page: 169-82   2012

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/B978-0-12-415931-0.00009-4

    PubMed

  105. Traffic jams reduce hydrolytic efficiency of cellulase on cellulose surface.

    Science (New York, N.Y.)   Vol. 333 ( 6047 ) page: 1279-82   2011.9

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1126/science.1208386

    PubMed

  106. High-speed atomic force microscopy reveals rotary catalysis of rotorless F₁-ATPase.

      Vol. 333 ( 6043 ) page: 755-8   2011.8

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1126/science.1205510

    PubMed

  107. Structural changes in bacteriorhodopsin in response to alternate illumination observed by high-speed atomic force microscopy.

    Shibata M, Uchihashi T, Yamashita H, Kandori H, Ando T

    Angewandte Chemie (International ed. in English)   Vol. 50 ( 19 ) page: 4410-3   2011.5

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1002/anie.201007544

    PubMed

  108. High-speed atomic force microscopy and biomolecular processes.

    Uchihashi T, Ando T

    Methods in molecular biology (Clifton, N.J.)   Vol. 736   page: 285-300   2011

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1007/978-1-61779-105-5_18

    PubMed

  109. High-speed atomic force microscopy shows dynamic molecular processes in photoactivated bacteriorhodopsin Reviewed

    Shibata Mikihiro, Yamashita Hayato, Uchihashi Takayuki, Kandori Hideki, Ando Toshio

    NATURE NANOTECHNOLOGY   Vol. 5 ( 3 ) page: 208-212   2010.3

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1038/NNANO.2010.7

    Web of Science

  110. Dynamics of bacteriorhodopsin 2D crystal observed by high-speed atomic force microscopy

    内橋 貴之

    JOURNAL OF STRUCTURAL BIOLOGY   Vol. 167 ( 153-158 ) page: .   2009

     More details

    Publishing type:Research paper (scientific journal)  

  111. High resonance frequency force microscope scanner using inertia balance support Reviewed

    Fukuma Takeshi, Okazaki Yasutaka, Kodera Noriyuki, Uchihashi Takayuki, Ando Toshio

    APPLIED PHYSICS LETTERS   Vol. 92 ( 24 )   2008.6

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1063/1.2951594

    Web of Science

  112. Visualization of intrinsically disordered regions of proteins by high-speed atomic force microscopy

    CHEMPHYSCHEM   Vol. 9 ( 13 ) page: 1859-1866   2008

     More details

    Publishing type:Research paper (scientific journal)  

    DOI: 10.1002/cphc.200800210

    PubMed

    CiNii Article

  113. High-speed AFM and nano-visualization of biomolecular processes

    内橋 貴之

    Pflugers Archiv -Eur. J. Physiol.   Vol. 456   page: .   2008

     More details

    Publishing type:Research paper (scientific journal)  

  114. High resonance frequency force microscope scanner using inertia balance support

    内橋 貴之

    APPLIED PHYSICS LETTERS   Vol. 92 ( 243119 ) page: .   2008

     More details

    Publishing type:Research paper (scientific journal)  

  115. High-speed atomic force microscopy for nano-visualization of dynamic biomolecular processes

    内橋 貴之

    PROGRESS IN SURFACE SCIENCE   Vol. 83 ( 337-437 ) page: .   2008

     More details

    Publishing type:Research paper (scientific journal)  

  116. High-speed atomic force microscopy for observing dynamic biomolecular processes.

    Ando T, Uchihashi T, Kodera N, Yamamoto D, Taniguchi M, Miyagi A, Yamashita H

    Journal of molecular recognition : JMR   Vol. 20 ( 6 ) page: 448-58   2007.11

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1002/jmr.843

    PubMed

  117. Tip-sample distance control using photothermal actuation of a small cantilever for high-speed atomic force microscopy.

    Yamashita H, Kodera N, Miyagi A, Uchihashi T, Yamamoto D, Ando T

    The Review of scientific instruments   Vol. 78 ( 8 ) page: 083702   2007.8

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1063/1.2766825

    PubMed

  118. High-speed atomic force microscopy for observing dynamic biomolecular processes

    内橋 貴之

    JOURNAL OF MOLECULAR RECOGNITION   Vol. 20 ( 448-458 ) page: .   2007

     More details

    Publishing type:Research paper (scientific journal)  

  119. Capacitance XAFS method: A new site-selective and microscopic X-ray absorption Spectroscopy Reviewed

    Ishii Masashi, Nakao Aiko, Uchihashi Takayuki

    PHYSICA SCRIPTA   Vol. T115   page: 97-101   2005

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    Web of Science

▼display all

Books 18

  1. 膜タンパク質工学ハンドブック

    内橋貴之( Role: Contributor ,  第1編 第2章15「高速原子間力顕微鏡によるタンパク質の構造ダイナミクス解析」)

    株式会社 エヌ・ティー・エス  2020.4  ( ISBN:978-4-86043-537-0

     More details

    Language:Japanese Book type:Scholarly book

  2. 自己修復・自己組織化材料の開発と応用事例

    内橋貴之( Role: Contributor ,  第5章 第12節「高速原子間力顕微鏡による分子の自己組織化過程のリアルタイムでの観察」)

    技術情報協会  2020.3  ( ISBN:978-4861047817

     More details

    Language:Japanese Book type:Scholarly book

  3. 実験医学増刊 Vol.36 No.20, 「生きてるものは全部観る! イメージングの選び方・使い方100」

    内橋貴之( Role: Contributor ,  第5章 走査型プローブ顕微鏡「原子間力顕微鏡 ⅰ.高速原子間力顕微鏡」)

    羊土社  2018.12  ( ISBN:978-4-7581-0375-6

  4. Compendium of Surface and Interface Analysis

    Takayuki Uchihashi( Role: Contributor)

    The Surface Science Society of Japan  2018 

     More details

    Responsible for pages:263-267   Language:English Book type:Scholarly book

  5. 光と生命の事典

    内橋貴之( Role: Contributor ,  第5章 「光による生命現象の計測」177節 高速原子間力顕微鏡)

    朝倉書店  2016.2  ( ISBN:978-4-254-17161-7

     More details

    Language:Japanese Book type:Scholarly book

  6. Noncontact Atomic Force Microscopy Vol.3

    Takayuyki Uchihashi, Noriyuki Kodera, and Toshio Ando( Role: Contributor ,  Chapter 22: High-speed Atomic Force Microscopy)

    Springer  2015 

     More details

    Responsible for pages:481-518   Language:English Book type:Scholarly book

  7. Atomic Force Microscopy in Nanobiology

    Takayuki Uchihahsi, Noriyuki Kodera, Toshio Ando( Role: Contributor ,  " Chapter 8: Development of High-speed AFM and Its Biological Applications)

    Pan Stanford Publishing  2014  ( ISBN:978-981-4411-59-2

     More details

    Total pages:437   Responsible for pages:143-176  

  8. 膜タンパク質構造研究

    内橋貴之, 安藤敏夫( Role: Contributor ,  23章: 原子間力顕微鏡による膜タンパク質のダイナミクス研究)

    化学同人  2013.10  ( ISBN:4759815619

     More details

    Language:Japanese Book type:Scholarly book

  9. Nanoscale Liquid Interfaces: Wetting, Patterning, and Force Microscopy at the Molecular Scale

    Toshio Ando, Takayuki Uchihashi( Role: Contributor ,  Chapter 19: High-speed AFM and Imaging of Biomolecular Processes)

    Pan Stanford Publishing  2013  ( ISBN:9789814316453

     More details

    Language:English Book type:Scholarly book

    DOI: 10.4032/9789814364485

  10. Single-molecule Studies of Proteins (Biophysics for the Life Sciences) Vol 2

    Takayuki Uchihashi, Noriyuki Kodera, Toshio. Ando( Role: Contributor ,  Chapter 5 : Nanovisualization of proteins in action using high-speed AFM)

    Springer  2013  ( ISBN:978-1-4614-4920-1

     More details

    Responsible for pages:119-147   Language:English Book type:Scholarly book

    DOI: 10.1007/978-1-4614-4921-8

  11. Atomic force microscopy in liquid: Biological Applications

    ( Role: Contributor ,  Toshio Ando, Takayuki Uchihashi, Noriyuki Kodera)

    Wiley-VCH Verlag GmbH  2012  ( ISBN:9783527327584

     More details

    Responsible for pages:189-209   Language:English Book type:Scholarly book

    DOI: 10.1002/9783527649808

  12. Life at the Nanoscale - Atomic force Microscopy of Live Cells

    Takayuki Uchihashi, Toshio Ando( Role: Contributor)

    Chapter 8 : High-speed atomic force microscopy for dynamic biological imaging  2011  ( ISBN:9789814267960

     More details

    Responsible for pages:163-184   Language:English Book type:Scholarly book

    DOI: 10.4032/9789814267977

  13. Atomic force microscopy in Biomedical Research: Methods and Protocols

    Takayuki Uchihashi, Toshio Ando( Role: Contributor ,  Chapter 18 : High-speed Atomic Force Microscopy and Biomolecular Processes)

    Humana Press  2011 

     More details

    Responsible for pages:285-300   Language:English Book type:Scholarly book

  14. 酵素利用技術大系

    内橋貴之, 安藤敏夫( Role: Contributor ,  第2編6節:AFMを用いた酵素反応解析)

    株式会社 エヌ・ティー・エス  2010.4  ( ISBN:4860432711

     More details

    Language:Japanese Book type:Scholarly book

  15. Single Molecule Dynamics in Life Science

    Toshio Ando, Takayuki Uchihashi, Noriyuki Kodera, Daisuke Yamamoto, Masaaki Taniguchi, Atsushi Miyagi, Hayato Yamashita( Role: Contributor ,  Chapter 12: High-speed atomic force microscopy for nano-visualization of biomolecular processes)

    Wiley-VCH Verlag GmbH  2008  ( ISBN:978-3-527-31288-7

     More details

    Responsible for pages:277-296   Language:English Book type:Scholarly book

  16. Scanning Probe Microscopy: Characterization, Nanofabrication and Device Application of Functional Materials (NATO SCIENCE SERIES II: Mathematics, Physics and Chemistry)

    Seizo Morita, Takayuki Uchihashi, Kenji Okamoto, Masayuki Abe, and Yasuhiro Sugawara(Nanoscale Contact Charging on a Silicon Oxide)

    Springer Netherlands  2005  ( ISBN:978-1-4020-3019-2

     More details

    Responsible for pages:289-308   Language:English Book type:Scholarly book

    DOI: 10.1007/1-4020-3019-3

  17. Fundamentals of Tribology and Bridging the Gap between Micro-and Micro/Nanoscales (NATO SCIENCE SERIES: Mathematics, Physics and Chemistry)

    Seizo Morita, Yasuhiro Sugawara, Kousuke Yokoyama, and Takayuki Uchihashi(Atomic Scale Origins of Force Interaction")

    Springer  2001  ( ISBN:978-94-010-0736-8

     More details

    Responsible for pages:103-120   Language:English Book type:Scholarly book

    DOI: 10.1007/978-94-010-0736-8

  18. Forces in Scanning Probe Methods" (NATO Advanced Science Institutes Series E: Applied Science -Vol.286)

    Yasuhiro Sugawara, Seizo Morita, Yoshinobu Fukano, Takayuki Uchihashi, Takahiro Okusako, Ayumi Chayahara, Yoshiki Yamanishi, and Takahiko Oasa( Role: Contributor ,  Time Dependence and its Spatial Distribution of Densely Contact-Electrified Electrons on a Thin Silicon Oxide)

    Springer  1995  ( ISBN:978-94-010-4027-3

     More details

    Responsible for pages:505-506   Language:English Book type:Scholarly book

    DOI: 10.1007/978-94-011-0049-6

▼display all

Presentations 15

  1. 高速原子間顕微鏡による膜輸送装置Secの動態観察

    長池航, 板家成良, 春山隆充, 塚崎智也, 内橋貴之

    日本生体エネルギー研究会第46回討論会  2020.12.10  日本生体エネルギー研究会

     More details

    Event date: 2020.12

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:金沢   Country:Japan  

  2. 高速原子間力顕微鏡を用いた光化学系IIの動態観察

    戸叶貴也, 加藤祐樹, 杉山翔吾, 野口巧, 内橋貴之

    日本生体エネルギー研究会第46回討論会  2020.12.10  日本生体エネルギー研究会

     More details

    Event date: 2020.12

    Language:Japanese   Presentation type:Poster presentation  

    Venue:金沢   Country:Japan  

  3. 「見て触って理解するタンパク質の動きと機能 ~高速原子間力顕微鏡技術の基礎 から生物応用まで~ 」 Invited

    内橋貴之

    慶應義塾大学 大学院講義「先端研究  2020.12.2  慶應義塾大学

     More details

    Event date: 2020.12

    Language:Japanese   Presentation type:Public lecture, seminar, tutorial, course, or other speech  

    Venue:オンライン   Country:Japan  

  4. Development of Tip-Scan Atomic Force Microscope for Stretching Elastic Substrates

    F.-Y. ChanR. Kurosaki, T. Uchihashi

    International Colloquium on Scanning Probe Microscopy (ICSPM28)  2020.12.10 

     More details

    Event date: 2020.12

    Language:English   Presentation type:Poster presentation  

    Country:Japan  

  5. 高速AFMによる酸素発生光化学系II のドメイン構造揺らぎの可視化 Invited

    内橋貴之

    ブルカージャパン オンラインシンポジウム 〜単一生体分子の動的プロセス評価に向けた高速AFM技術〜  2020.12.15  ブルカージャパン

     More details

    Event date: 2020.11

    Language:Japanese   Presentation type:Public lecture, seminar, tutorial, course, or other speech  

    Venue:オンライン   Country:Japan  

  6. 高速原子間力顕微鏡でタンパク質の一分子動態を可視化する Invited

    内橋貴之

    新学術領域合同シンポジウム-ソフトロボット学と発動分子科学の境界  2020.11.4 

     More details

    Event date: 2020.11

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Venue:オンライン   Country:Japan  

  7. 高速原子間力顕微鏡で可視化する生体・人工分子のダイナミクス Invited

    内橋貴之

    岡山大学大学院医歯薬学総合研究科(薬学系) 先端薬学特論 公開セミナー  2020.10.9  岡山大学大学院医歯薬学総合研究科

     More details

    Event date: 2020.10

    Language:Japanese   Presentation type:Public lecture, seminar, tutorial, course, or other speech  

    Venue:オンライン   Country:Japan  

  8. Observation of Substrate Binding Sec Translocon and Structural Change of SecA with HS-AFM International conference

    Wataru Nagaike, Takamitsu Haruyama, Tomoya Tsukazaki and Takayuki Uchihashi

    2020.9.16 

     More details

    Event date: 2020.9

    Language:English   Presentation type:Poster presentation  

  9. Chasing the transformation between monomer and dimer structure of cadherin anchored to supported lipid bilayer by high-speed AFM International conference

    Shigetaka Nishiguchi, Hiroki Oda and Takayuki Uchihashi

    2020.9.16 

     More details

    Event date: 2020.9

    Language:English   Presentation type:Poster presentation  

    Country:Japan  

  10. Structures of heliorhodopsin and schizorhodopsin elucidate the structural diversity of microbial rhodopsins

    Wataru Shihoya, Keiichi Inoue, Singh Manish, Akimitsu Higuchi, Masae Konno, Rei Yoshizumi, Takayuki Uchihashi, Hideki Kandori and Osamu Nureki

    2020.9.16 

     More details

    Event date: 2020.9

    Language:English   Presentation type:Poster presentation  

    Country:Japan  

  11. Characterization of the enzymatic property and structural dynamics of the T3SS ATPase from Enteropathogenic Escherichia coli International conference

    Aya Suzuki, Hiroshi Ueno, Ryo Kurosaki, Takayuki Uchihashi and Hiroyuki Noji

    2020.9.16 

     More details

    Event date: 2020.9

    Language:English   Presentation type:Poster presentation  

    Country:Japan  

  12. HS-AFM observation of Amyloid β elongation and inhibition by antibodies International conference

    Shogo Miyajima, Maho Yagi-Utsumi, Takayuki Uchihashi and Koichi Kato

    2020.9.16 

     More details

    Event date: 2020.9

    Language:English   Presentation type:Poster presentation  

    Country:Japan  

  13. HS-AFM as a versatile tool to study dynamical and mechanical properties of proteins International conference

    Christian Ganser, Kimitoshi Takeda, Ryota Iino, Koichi Kato and Takayuki Uchihashi

    2020.9.18 

     More details

    Event date: 2020.9

    Language:English   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  14. Structural insights into the mechanism of rhodopsin phosphodiesterase Invited International conference

    Wataru Shihoya, Tatsuya Ikuta, Masahiro Sugiura, Kazuho Yoshida, Masahito Watari, Takaya Tokano, Kota Katayama, Satoshi Tsunoda, Takayuki Uchihashi, Hideki kandori and Osamu Nureki

    2020.9.17 

     More details

    Event date: 2020.9

    Language:English   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  15. High-speed atomic force microscopy – a versatile tool to study protein dynamics and more

    Christian Ganser, Kimitoshi Takeda, Ryota Iino, Koichi Kato and Takayuki Uchihashi

    2020.2.7 

     More details

    Event date: 2020.2

    Language:English   Presentation type:Oral presentation (general)  

    Country:Japan  

▼display all

KAKENHI (Grants-in-Aid for Scientific Research) 16

  1. 機械刺激を加えた単一タンパク質の構造および力学特性応答の高速AFM解析

    Grant number:21H01772  2021.4 - 2024.3

    内橋 貴之

      More details

    Authorship:Principal investigator 

    Grant amount:\17420000 ( Direct Cost: \13400000 、 Indirect Cost:\4020000 )

  2. 動態イメージングによるタンパク質膜透過を担う発動分子複合体の作動メカニズムの解明

    Grant number:21H00393  2021.4 - 2023.3

    新学術領域研究(研究領域提案型)

    内橋 貴之

      More details

    Authorship:Principal investigator 

    Grant amount:\5980000 ( Direct Cost: \4600000 、 Indirect Cost:\1380000 )

  3. Study for the mechanism of functional expression and dysfunction of membrane proteins through direct observations of single molecular fluctuation

    Grant number:20K07279  2020.4 - 2023.3

      More details

    Authorship:Coinvestigator(s) 

  4. 高速AFMで可視化する外的刺激により誘起される多孔性結晶表面の構造ダイナミクス

    Grant number:20H04669  2020.4 - 2022.3

    新学術領域研究(研究領域提案型)

    内橋 貴之

      More details

    Authorship:Principal investigator 

    Grant amount:\5330000 ( Direct Cost: \4100000 、 Indirect Cost:\1230000 )

    吸着分離材、不均一系触媒などの多くの応用が期待されている多孔性結晶は、ゲスト分子の吸着や圧力、電場などの外的刺激に応答して結晶構造が柔軟に変化する。本研究では、外的刺激に応答する多孔性結晶表面のナノスケールダイナミクス現象を高速原子間力顕微鏡(AFM)で直接可視化し、I: MMFやMOFなど多孔性結晶へのゲスト分子の吸着と格子歪の結晶表面伝搬の可視化、II: MMF表面での金ナノ粒子の成長過程の追跡、III: 局所力学刺激による格子歪誘起と選択的ゲスト分子の吸着制御の3項目について研究を行い、多孔性結晶の機能設計や性能向上のために基盤的知見を得る。

  5. 再構成アプローチで解明するダイナミンの膜切断機構とその破綻に起因する疾患発症機序

    Grant number:19KK0180  2019.10 - 2023.3

    国際共同研究加速基金(国際共同研究強化(B))

    竹田 哲也

      More details

    Authorship:Coinvestigator(s) 

    本研究では、日本国内5名(代表者:竹田、分担者:内橋、谷、竹居、西上)と、海外共同研究者(Harvey McMahon、MRC分子生物学研究所)で構成する学際的かつ国際的な共同研究チームを組織し、ダイナミンによる膜切断機構の作動原理とBARドメイン蛋白質による制御機構、さらにダイナミンの機能破綻によって起こる難治性疾患の発症機序を、in vitro再構成系を用いた「ボトムアップ型」の構成生物学的なアプローチで解明する。
    本研究の目的は、ダイナミンによる膜切断機構およびBARドメイン蛋白質による制御機構、さらにダイナミンの異常に起因する難治性疾患の発症機序を明らかにすることである。この目的の達成に向け、代表者(竹田)が細胞生物学的に解析を進めている、ダイナミンやBARドメイン蛋白質による「複雑な」細胞内膜リモデリング機能を、海外共同研究者(McMahon)や分担者(竹居)が開発したin vitro再構成系を用いて「単純化」して再現し、電子顕微鏡による構造解析(竹居)、高速AFM(内橋)や蛍光偏光顕微鏡(谷)を用いた分子動態イメージング解析を行う。さらに、得られたデータの定量的解析をもとに、ダイナミンによる膜切断機構の数理モデルを使った検証を試み(西上)、ダイナミンによる膜切断機構の作動原理とその破綻による疾患発症機序の解明を目指す。
    初年度はキックオフミーティングを開催し、チームメンバーによる議論を通じて、プロジェクトの進捗状況および今後の研究の方向性について確認した。また海外共同研究者であるHarvey McMahon博士を、第58回日本生物物理学会年会に招聘し、その際に日英全てのメンバーによるミーティングを行うことを決定した。代表者(竹田)は、初年度に計3ヶ月間McMahon研究室に滞在し、研究を行う予定であったが、新型コロナ感染拡大の影響により、渡英は次年度以降に行うこととした。その間、代表者(竹田)はダイナミンファミリーやBARドメイン蛋白質の精製蛋白質の調製を行い、分担者(竹居、内橋)と共同で、電子顕微鏡および高速AFMを用いて膜リモデリング分子のin vitro解析の準備を進めている。また分担者(谷、西上)は、分子動態イメージングとそのデータに基づいた数理モデリングの準備を進めている。
    初年度はキックオフミーティングを開催し、チームメンバーによる議論を通じて、プロジェクトの進捗状況と今後の方向性に関して確認した。また海外共同研究者であるHarvey McMahon博士(MRC-LMB)を、第58回日本生物物理学会年会に招聘し、その際に日英の全てのメンバーによるミーティングを行うことを決定した。代表者(竹田)は、初年度に計3ヶ月間McMahon研究室に滞在し、疾患型ダイナミンのin vitro膜リモデリング解析を行う予定であったが、新型コロナ感染拡大の影響のため、渡英は次年度以降に行うこととした。その間、代表者(竹田)は、ダイナミンファミリーやBARドメイン蛋白質の精製蛋白質の調製を進めており、分担者(竹居、内橋)と共同で、膜リモデリングのin vitro再構成系の電子顕微鏡および高速AFMによる解析を進めている。また分担者(谷、西上)は、分子動態イメージングとそのデータに基づいた数理モデリングの準備を進めている。以上のように本研究はおおむね順調に進展している。
    代表者(竹田)は引き続き研究の統括およびダイナミンファミリーやBARドメイン蛋白質の精製蛋白質の調製や細胞生物学的解析を行う。また分担者は、海外共同研究者(McMahon)や分担者(竹居)が開発した膜リモデリングのin vitro再構成系を用い、電子顕微鏡による構造解析(竹居)、高速AFMによる分子動態解析(内橋)、蛍光偏光顕微鏡解析(谷)と数理モデリング(西上)を行う。代表者(竹田)は計3ヶ月間McMahon研究室に滞在し、疾患型ダイナミンのin vitro膜リモデリング解析を行うと共に、MRC分子生物学研究所の研究者とのネットワーキングを積極的に行い、今後の研究発展に不可欠な、学術的・人的な基盤づくりに努める。今後の具体的な実験として、1.ダイナミンによる膜切断機構の解明:(1)電子顕微鏡および高速AFMによる分子構造・動態解析;(2)蛍光偏光顕微鏡による膜切断過程におけるダイナミンの分子配向解析、2.BARドメイン蛋白質による膜切断制御機構の解明:(1)BARドメイン蛋白質によるダイナミンの膜切断制御機構の解析;(2)BARドメイン蛋白質による新規膜切断機構の解析、3.ダイナミン異常疾患の発症機序の解明を計画している。

  6. Cooperative regulation of cytoskeleton and membrane dynamics by novel mechanism of dynamin

    Grant number:19H03225  2019.4 - 2022.3

      More details

    Authorship:Coinvestigator(s) 

  7. 発動分子の化学-力学エネルギー変換機構の解明に資する高速AFM技術の開発

    Grant number:19H05389  2019.4 - 2021.3

    新学術領域研究(研究領域提案型)

    内橋 貴之

      More details

    Authorship:Principal investigator 

    Grant amount:\5980000 ( Direct Cost: \4600000 、 Indirect Cost:\1380000 )

    本領域で創成された発動分子やその集積体の構造動態や動的相互作用、秩序形成のダイナミクス観察に関して共同研究を推進する。同時に、計測技術・シミュレーション・理論班との協働により発動分子の作動原理を実験・理論両面から明らかにする。一方で、回転分子モーターV-ATPaseに焦点を絞り、高速AFM/一分子蛍光顕微鏡を用いてATPの結合と解離の検出と同時に分子の構造変化を測定する方法を確立し、化学-力学エネルギー変換の分子機構を明らかにする。また、高速AFM/一分子蛍光顕微鏡装置に実装する多機能基板の開発も進め、外部刺激による発動分子の動態応答に関する研究を推進する。
    i) DNA修復酵素MRE11/RAD50/NBS1(MRN)の動態解析:ヒトのDNA修復を担うMRE11/RAD50/NBS1複合体(MRN)の構造を高速AFMで解析した。従来、Rad50ダイマーはフック部位が開いた開環構造を取と考えられてきたが、高速AFMにより、MRN複合体の多くは閉じたリング構造を形成し、また、定説とは逆側のヘッドと呼ばれる球状ドメインで開きアーム部の構造も異なることを明らかにした。ヒトのみならず酵母や大腸菌のMRNホモログの分子構造を解析し、新たに発見されたヘッド部位でのリング開閉運動が種を超えて保存されていることも明らかにした。さらに、酵母細胞中のMRNのフックをSMCタンパク質のヒンジドメインと入れ替えたキメラMRNを作製し、キメラMRが酵母のDNA損傷を修復可能であることを見いただした(Tatebe et al, Nat. Commun. 2020)。
    ii) タンパク質分子針と脂質二重膜との相互作用解析(A01-3上野GおよびA01川野Gとの領域内共同研究)バクテリオファージ T4の細胞膜貫通部位から作製されあた人工分針の脂質二重層膜との動的相互作用を高速AFM観察し、分子針が脂質膜に貫入していく様子を観察することに成功した(Ueno, Nanoscale 2020)。
    iii) ゲル微粒子のタンパク質取り込み過程の観察( B01鈴木Gとの領域内共研究)ハイドロゲル微粒子にタンパク質が取り込まれる過程を観察し、ゲル微粒子の構造と電荷状態に依存して取り込まれるタンパク質のサイズや機構が異なることを明らかにした(Matsui et al, Chem. Commun. 2019)。
    本研究課題では、高速原子間力顕微鏡(AFM)による一分子動態計測を通し、発動分子の機能発現機構を明らかにする。また、高速AFM装置を基盤とした発動分子の化学-力学共役機構の解明に資する新規技術を開発することにある。高速AFMを用いた発動分子の機能動態解析は、研究実績の概要に記載しているように、DNA修復酵素や人工分針の機能動態、ハイドロゲル微粒子のタンパク質取り込み挙動などの観察に成功した。この他にも、ヘムタンパク質の集積過程(Hirayama et al, JACS, 2020 )や酵母プリオンのアミロイド構造 (Konnno et al, PNAS, 2020)のダイナミクス観察に成功して成果をあげつつある。領域内共研究も積極的に実施しており、現在8グループと共同研究を進めており、3グループとは既に共同研究の成果を論文として公表している。以上から、高速AFMを利用した発動分子のダイナミクス観察は順調に進捗している。
    発動分子の化学-力学共役機構の解明に向けた新規技術に関しては、今年度はプラットフォームとなる探針走査型高速AFMの開発に注力した。設計・部品製作は終了し、現在組み立てと動作確認を行っているところである。現在までに、ガラス表面や脂質二重膜をテスト試料として高速AFM観察を行えるところまで確認できている。
    従来型の高速AFMを用いた発動分子の機能動態解析はこれまで同様に、領域家内共同研究を含めて積極的に実施していくことで、発動分子の創成に向けた領域の研究進展に尽力する。特に、最近A01上野Gと共同研究を開始した六量体リング状ATPaseのリング内協同性と化学-力学共役機構の解明を中心に行なう。装置開発に関しては、7月までに探針走査型高速AFMの立ち上げと操作性向上のためのソフトウェア開発を終える。次に、基板操作用のマニピュレータの製作に取り掛かる。弾性変形可能なPDMAを基板として、基板の力学操作が可能なことを確かめす。これに成功すれば、PDMS基板に微小管を固定し、微小管に伸長あるいは圧縮の力学負荷を印加しながら、キネシンの滑走状態を高分解能に観察し、レールタンパク質の構造変調とモータータンパク質の運動活性について研究する。また、PDMSとパターン基板を用いた中空脂質二重膜形成のためのパターン化井戸構造を持つ基板作製検討し、人工分子の脂質膜への再構成と膜内拡散のダイナミクス観察のための基盤技術を完成させる。

  8. High-speed AFM study on functional modulation of kinesin caused by structural defects of mictoruble

    Grant number:18H01837  2018.4 - 2021.3

      More details

    Authorship:Principal investigator 

    Grant amount:\17550000 ( Direct Cost: \13500000 、 Indirect Cost:\4050000 )

  9. Development of analytical techniques for structural property dynamics based on high-speed AFM

    Grant number:18H04512  2018.4 - 2020.3

    Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

      More details

    Authorship:Principal investigator 

    Grant amount:\5200000 ( Direct Cost: \4000000 、 Indirect Cost:\1200000 )

  10. Development of high-speed AFM/single-molecule FRET to visualize synthesis and degradation of carbohydrate chain

    Grant number:17K19519  2017.6 - 2019.3

    Grant-in-Aid for Challenging Research (Exploratory)

    Uchihashi Takayuki

      More details

    Authorship:Principal investigator 

    Grant amount:\6240000 ( Direct Cost: \4800000 、 Indirect Cost:\1440000 )

    We have incorporated an image-splitting optical system into a combined high-speed AFM/ total-reflection fluorescent microscopy system which enables us to perform high-speed AFM imaging and single-molecule FRET simultaneously. We prepared the fluorescent labeling sample for single-molecule FRET measurements for bacterial chondroitin polymerase K4CP and glycoside hydrolase cellulase, TrCel6A, and succeeded in measuring changes of the FRET efficiently due to the extension of the chondroitin chain and the structural change of the cellulase. Although we tried to observe these molecule with the combined high-speed AFM and single-molecule FRET system, in the face of various problems, we have not reached the success within the period. However we are now establishing the optimum measurement conditions for the simultaneous observation and thus assume that we can gain novel insights about molecular mechanisms with detailed single-molecule analysis using the combined system.

  11. Elucidation of mechanical property and self-repair mechanism of microtubule with high-speed AFM

    Grant number:17F17701  2017.4 - 2019.3

      More details

    Authorship:Other 

  12. 高速AFM計測によるKaiタンパク質のロバストな概日周期発生機構の解明

    Grant number:16H00758  2016.4 - 2018.3

    新学術領域研究(研究領域提案型)

    内橋 貴之

      More details

    Authorship:Principal investigator 

    Grant amount:\6370000 ( Direct Cost: \4900000 、 Indirect Cost:\1470000 )

    Kaiタンパク質の動的相互作用: KaiCのリン酸化状態に依存してKaiAとの相互作用が概日周期的に変動することを見出した(Phase Dependent Differential Affinity: PDDAと名付けた )。KaiCのリン酸化概日周期について、実験で得られたパラメーターを用いて数理シミュレイションを行い、PDDAが概日周期にどのような影響を及ぼすのかを調べた。PDDAが無い場合には、KaiAとKaiCの濃度比が変動すると概日周期が消失するのに対して、PDDAがある場合には概日周期が維持される濃度比が3倍程度大きくなった。このことから、PDDAは細胞内でのタンパク質濃度の揺らぎに対するKaiシステムの頑強性に寄与していることが明らかになった。また、温度制御下でKaiA-KaiCの相互作用を調べたところ、25-29℃の温度範囲では動的親和性に大きな変化は見られえず、30℃以上では、KaiAとKaiCの親和性が大きく変化することがわかった。
    プロテアソームα7ホモ14量体のα6による2ステップ解体過程:領域内共同研究としてプロテアソーム構成タンパク質α7ホモ14量体がα6により解体される過程を観察した。α7-14量体をアミノシランで化学修飾したマイカに強固に吸着させると14量体が自発的に7量体に分離する様子が見られた。さらに,α7-7量体リングの中心孔にα6サブユニットが結合・解離を繰り返し、時間経過とともにα6が中心孔に強固に結合することが分かった。また、積層した7量体リング間に隙間が経時的に生じ、そこにα6が結合する様子が観察された。これらのことから、α7-14量体のα6サブユニットによる解体は、リング積層間隙へのα6の結合と解離、7量体リング中心孔へのα6の強固な結合によるダブルリングの再生阻止の2段階の過程を経ていることを明らかにした。
    29年度が最終年度であるため、記入しない。
    29年度が最終年度であるため、記入しない。

  13. 高速AFMで明らかにする真正細菌型イオンポンプロドプシンの多量体構造と機能動態

    Grant number:16H00830  2016.4 - 2018.3

    新学術領域研究(研究領域提案型)

    内橋 貴之

      More details

    Authorship:Principal investigator 

    Grant amount:\6890000 ( Direct Cost: \5300000 、 Indirect Cost:\1590000 )

    1.イオンポンプロドプシンの多量体構造の決定: 様々な微生物型ロドプシンを高速AFM法と円二色性(CD)分光法により網羅的に解析し、脂質膜に再構成された状態での多量体構造を決定した。高速AFM観察により、GR、KR2、FR、KrActR、QsActR等の真性細菌で見出された微生物型ロドプシンはリング状の5量体を形成しており、GPRは5量体と6量体が共存していることがわかった。また、センサリーロドプシンであるSRII、ハロロドプシンNpHRなどの古細菌型ロドプシンは三量体であることがわかった。一方、内向きH+ポンプであるPoXeRやセンサリーロドプシンASRは真性細菌で発見された微生物型ロドプシンであるが、アミノ酸残基の相同性からは古細菌型に分類され、実際多量体構造は三量体であった。また、CD分光で観察される三量体と五量体に特徴的なスペクトルは多量体構造に依存したレチナールの配向で説明できることもわかった。これらの結果から、微生物型プロドプシンの多量体構造は進化系統樹と深く関わっている一方、イオンポンプのイオン種や向きと相関がないことがわかった。
    2.KR2の光誘起構造変化の観察の観察: 外向きNa+ポンプであるKR2の光誘起による構造変化の観察に向けて、KR2五量体の観察面の決定およい高解像観察に適した試料の調製を行った。野生型に比べて光サイクルが100以上遅いN112A変異体を高解像観察しながら、周期的な光照射を照射したが明瞭な構造変化は観察できなかった。このことから、BRとは異なり、KR2の光サイクル中には大規模な構造変化は起きていない可能性が示唆された。
    29年度が最終年度であるため、記入しない。
    29年度が最終年度であるため、記入しない。

  14. Analysis of the mechanism of efficient degradation of crystalline substrate by cellulases from Actinomycetes

    Grant number:15K07383  2015.4 - 2018.3

    Uchiyama Taku

      More details

    Authorship:Collaborating Investigator(s) (not designated on Grant-in-Aid) 

    Toward to the realization of “Biorefinery”, we aimed to increase the efficiency of enzymatic saccharification, and attempted to elucidate the enzymatic reaction mechanism involved in cellulases efficient crystalline cellulose degradation. Specifically, we attempted to elucidate such a mechanism through the biochemical experiment, HS-AFM observation, and protein crystal structure analysis of cellulase SaCel6B derived from Actinomycetes. As a result of experiments and observations, we found two amino acid residues involved in efficient crystalline cellulose degradation of cellulase SaCel6B and clarified the importance of these residues. Based on information of such amino acid residues, it could be possible to try to discover cellulases with highly saccharification efficiency or synthesize novel cellulases with high efficiency of saccharification.

  15. Development of variable-temperature high-speed AFM and its application to temperature-dependent ATPases

    Grant number:15H03540  2015.4 - 2018.3

    UCHIHASHI TAKAYUKI

      More details

    Authorship:Principal investigator 

    Grant amount:\16770000 ( Direct Cost: \12900000 、 Indirect Cost:\3870000 )

    We have developed variable-temperature high-speed atomic force microscopy (VT-HS-AFM) with which the experimental temperature can be controlled ranging from a room temperature to around 40 ℃. The performance of VT-HS-AFM was confirmed by observation of fluidity of DPPC lipid bilayer with the phase-transition temperature of 41℃ from gel phase to liquid crystal phase. We applied the VT-HS-AFM system to observation of a flagellar protein, FliI, which is an ATPase with the activity optimum temperature over 40℃. HS-AFM images captured oligomerization processes of the FliI monomers to the hexamer and also conformational changes of the oligomers. Also we observed temperature dependent interaction between KaiC and KaiA which are proteins responsible to the circadian rhythm of Cyanobacteria.

  16. Development of advanced high-speed AFM and analysis of proteins' action mechanism

    Grant number:26119003  2014.7 - 2019.3

    Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

    Ando Toshio

      More details

    Authorship:Collaborating Investigator(s) (not designated on Grant-in-Aid) 

    The aim of this study is largely classified into two: (1) Extensively exploring a new research field of “dynamic structural biology” that seeks to film protein molecules in dynamic action with high-speed AFM, not only by conducting the study by myself but also by collaborating with researchers of the project group as well as external researchers, and (2) enabling observation of new molecular phenomena that have been impossible with the current high-speed AFM, by advancing the capability of high-speed AFM. For the aim (1), our instruments were opened to these researchers. Through many collaborations, we succeeded in observing a variety of proteins, providing mechanistic insights into their molecular processes. For the aim (2), we succeeded in developing high-speed AFM combined with optical tweezers and super-resolution fluorescence microscopy based on high-speed AFM. The actual application studies of these new instruments are subjects to be done in the future.

▼display all