Faculty Profiles - SAKAI Kiyoshi

Papers - SAKAI Kiyoshi

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Implant-supported fixed prostheses with cantilever: a systematic review and meta-analysis. Reviewed International journal

Kondo Y, Sakai K, Minakuchi H, Horimai T, Kuboki T, JSOI Clinical Guideline Working Group collaborators

International journal of implant dentistry Vol. 10 ( 1 ) page： 57 2024.11

Conditioned medium of human mesenchymal stem cells affects stem cell senescence in osteoporosis. Reviewed International coauthorship International journal

Liu K, Sakai K, Watanabe J, Dong J, Maruyama H, Li X, Hibi H

Biochemical and biophysical research communications Vol. 711 page： 149858 2024.4

Cytoprotective role of human dental pulp stem cell-conditioned medium in chemotherapy-induced alopecia. Reviewed International journal

Chen H, Yamaguchi S, Wang Y, Kaminogo K, Sakai K, Hibi H

Stem cell research & therapy Vol. 15 ( 1 ) page： 84 2024.3

Cellular senescence is associated with osteonecrosis of the femoral head while mesenchymal stem cell conditioned medium inhibits bone collapse. Reviewed International journal

Okamoto M, Nakashima H, Sakai K, Takegami Y, Osawa Y, Watanabe J, Ito S, Hibi H, Imagama S

Scientific reports Vol. 14 ( 1 ) page： 3329 2024.2

Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal) Publisher：Scientific Reports

Osteonecrosis of the femoral head (ONFH) is a type of ischemic osteonecrosis that causes pain, loss of function, and femoral head collapse. Here, we analyzed samples of femoral heads excised from patients with ONFH to clarify the relationship between ischemic osteonecrosis and cellular senescence. X-gal staining was strong and p16INK4a-positive cells were abundant in the transitional region of ONFH. The β-galactosidase-positive cells in the transitional region were also positive for nestin, periostin, or DMP-1. In contrast, no β-galactosidase-positive cells were detected in the healthy region. The senescence-associated p16INK4a, p21, and p53 were upregulated in ONFH tissue. We also examined and analyzed a mouse ischemic femoral osteonecrosis model in vivo to verify the association between ONFH and cellular senescence. Human mesenchymal stem cell-conditioned medium (MSC-CM) was administered to determine its therapeutic efficacy against cellular senescence and bone collapse. MSC-CM reduced the number of senescent cells and downregulated the aforementioned senescence-related genes. It also decreased the number of empty lacunae 4 weeks after ischemia induction and promoted bone formation. At 6 weeks post-surgery, MSC-CM increased the trabecular bone volume, thereby suppressing bone collapse. We conclude that cellular senescence is associated with ONFH and that MSC-CM suppresses bone collapse in this disorder.

DOI： 10.1038/s41598-024-53400-w

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PubMed

Langerhans cell histiocytosis of single-system multifocal bone, including the mandible, in a 22-month-old child: A case report Reviewed International journal

Kiyoshi Sakai, Noriyuki Yamamoto, Satoshi Yamaguchi, Kazuto Okabe, Yoshiro Koma, Hideharu Hibi

Oral Science International 2022.6

Dental pulp stem cell-derived small extracellular vesicle in irradiation-induced senescence Reviewed International coauthorship International journal

Dong Jiao, Sakai Kiyoshi, Koma Yoshiro, Watanabe Junna, Liu Kehong, Maruyama Hiroshi, Sakaguchi Kohei, Hibi Hideharu

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS Vol. 575 page： 28 - 35 2021.10

Conditioned medium from stem cells derived from human exfoliated deciduous teeth ameliorates NASH via the Gut-Liver axis. Reviewed International journal

Muto H, Ito T, Tanaka T, Yokoyama S, Yamamoto K, Imai N, Ishizu Y, Maeda K, Honda T, Ishikawa T, Kato A, Ohshiro T, Kano F, Yamamoto A, Sakai K, Hibi H, Ishigami M, Fujishiro M

Scientific reports Vol. 11 ( 1 ) page： 18778 2021.9

Coexistence of Sjogren's syndrome and bilateral ranulas: A case report and review of the literature Reviewed International journal

Koma Yoshiro, Fujimoto Takehiro, Sakai Kiyoshi, Sugimura Yukiko, Yamaguchi Satoshi, Hibi Hideharu

ORAL SCIENCE INTERNATIONAL 2021.9

Language：English Publishing type：Research paper (scientific journal) Publisher：Oral Science International

Sjögren's syndrome and ranulas are relatively frequently occurring diseases. However, when they occur simultaneously, its clinical features and pathogenesis remain unclear. Here, we report a patient with bilateral ranulas. Magnetic resonance imaging at diagnosis revealed a “salt and pepper” appearance in bilateral parotid glands, leading a diagnosis of asymptomatic Sjögren's syndrome. She was successfully treated with excision of the left sublingual gland and sclerotherapy. A literature review suggests that this coexistence may not be very rare. In addition, it may be characterized by a high prevalence of bilateral ranulas.

DOI： 10.1002/osi2.1121

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Hyperparathyroidism-jaw tumor syndrome with bilateral ossifying fibromas in the mandible: A case report Reviewed International journal

Journal of Oral and Maxillofacial Surgery, Medicine, and Pathology 2021.7

Extracellular Vesicles of Stem Cells to Prevent BRONJ Reviewed International journal

Watanabe J, Sakai K, Urata Y, Toyama N, Nakamichi E, Hibi H

Journal of Dental Research Vol. 99 ( 5 ) page： 552 - 560 2020.5

先天性無フィブリノゲン血症患者の抜歯術における周術期管理を行った1例 Reviewed

岡部一登, 土屋周平, 酒井陽, 松下義泰, 杉本圭佑, 日比英晴

日本口腔外科学会雑誌 Vol. 65 ( 8 ) page： 507 - 512 2019.8

Language：Japanese Publishing type：Research paper (scientific journal) Publisher：(公社)日本口腔外科学会

13歳男子。右下6番根尖性歯周炎に起因した下顎骨骨膜下膿瘍および頬部蜂窩織炎と診断した。先天性無フィブリノゲン(Fbg)血症のため消炎処置前にFbg製剤2gを投与し、歯肉の腫脹部より膿瘍切開術を施行した。同日よりアモキシシリン投与を開始し、翌日より切開部からの洗浄を継続し、7日後には顎下部や口腔内の腫脹は消退した。翌月に右下6番を抜歯し、抜歯後は事前に製作した創部保護床を終日装着させた。Fbg製剤は血中Fbg濃度100mg/dL以上を目標に抜歯当日3g、抜歯後1日目に2g、抜歯後2日目に2g投与した。抜歯後2日目には抜歯窩の縫合糸は脱落していたが幼若な血餅で満たされ、引き続き創部保護床は終日装着したままとして退院した。抜歯後8日目に創部保護床を除去し、処置後の異常出血はなく、治癒の遅延も認めなかった。

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Identification of the Novel Tooth-Specific Transcription Factor AmeloD Reviewed International coauthorship

He B, Chiba Y, Li H, de Vega S, Tanaka K, Yoshizaki K, Ishijima M, Yuasa K, Ishikawa M, Rhodes C, Sakai K, Zhang P, Fukumoto S, Zhou X, Yamada Y

Journal of Dental Research Vol. 98 ( 2 ) page： 234 - 241 2019.2

Dental pulp-derived stem cell conditioned medium to regenerate peripheral nerves in a novel animal model of dysphagia. Reviewed International journal

Takeshi Tsuruta, Kiyoshi Sakai, Junna Watanabe, Wataru Katagiri, Hideharu Hibi

PLOS ONE Vol. 13 ( 12 ) page： e0208938 2018.12

Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal)

In nerve regeneration studies, various animal models are used to assess nerve regeneration. However, because of the difficulties in functional nerve assessment, a visceral nerve injury model is yet to be established. The superior laryngeal nerve (SLN) plays an essential role in swallowing. Although a treatment for SLN injury following trauma and surgery is desirable, no such treatment is reported in the literature. We recently reported that stem cells derived from human exfoliated deciduous teeth (SHED) have a therapeutic effect on various tissues via macrophage polarization. Here, we established a novel animal model of SLN injury. Our model was characterized as having weight loss and drinking behavior changes. In addition, the SLN lesion caused a delay in the onset of the swallowing reflex and gain of laryngeal residue in the pharynx. Systemic administration of SHED-conditioned media (SHED-CM) promoted functional recovery of the SLN and significantly promoted axonal regeneration by converting of macrophages to the anti-inflammatory M2 phenotype. In addition, SHED-CM enhanced new blood vessel formation at the injury site. Our data suggest that the administration of SHED-CM may provide therapeutic benefits for SLN injury.

DOI： 10.1371/journal.pone.0208938

他家骨髄由来間葉系細胞の移植による骨再生の検討

渡邊純奈, 片桐渉, 大杉将嗣, 酒井陽, 岡部一登, 椙村有紀子, 坂口晃平, 鶴田剛士, 日比英晴

日本口腔インプラント学会誌 Vol. 31 ( 2 ) page： E75 - E75 2018.6

Mediator 1 Contributes to Enamel Mineralization as a Coactivator for Notch1 Signaling and Stimulates Transcription of the Alkaline Phosphatase Gene Reviewed International coauthorship

Keigo Yoshizaki, Lizhi Hu, Thai Nguyen, Kiyoshi Sakai, Masaki Ishikawa, Ichiro Takahashi, Satoshi Fukumoto, Pamela Den Besten, Daniel D. Bikle, Yuko Oda, Yoshihiko Yamada

JOURNAL OF BIOLOGICAL CHEMISTRY Vol. 292 ( 33 ) page： 13531-13540 2017.8

ラット末梢性嚥下障害モデルを用いた乳歯歯髄幹細胞由来成長因子の治療効果の検討 International journal

鶴田剛士, 片桐渉, 大杉将嗣, 酒井陽, 若山有紀子, 坂口晃平, 渡邊純奈, 日比英晴

日本口腔科学会雑誌 Vol. 66 ( 2 ) page： 207 - 208 2017.7

Pannexin 3 and connexin 43 modulate skeletal development through their distinct functions and expression patterns Reviewed International coauthorship International journal

Masaki Ishikawa, Geneva L. Williams, Tomoko Ikeuchi, Kiyoshi Sakai, Satoshi Fukumoto, Yoshihiko Yamada

JOURNAL OF CELL SCIENCE Vol. 129 ( 5 ) page： 1018 - 1030 2016.3

Language：English Publishing type：Research paper (scientific journal) Publisher：COMPANY OF BIOLOGISTS LTD

Pannexin 3 (Panx3) and connexin 43 (Cx43; also known as GJA1) are two major gap junction proteins expressed in osteoblasts. Here, we studied their functional relationships in skeletal formation by generating Panx3(-/-) and Panx3(-/-); Cx43(-/-) mice and comparing their skeletal phenotypes with Cx43(-/-) mice. Panx3(-/-) mice displayed defects in endochondral and intramembranous ossification, resulting in severe dwarfism and reduced bone density. The skeletal abnormalities of Panx3(-/-); Cx43(-/-) mice were similar to those in Panx3(-/-) mice. The gross appearance of newborn Cx43(-/-) skeletons showed no obvious abnormalities, except for less mineralization of the skull. In Panx3(-/-) mice, proliferation of chondrocytes and osteoblasts increased and differentiation of these cells was inhibited. Panx3 promoted expression of osteogenic proteins such as ALP and Ocn (also known as ALPL and BGLAP, respectively), as well as Cx43, by regulating Osx (also known as SP7) expression. Panx3 was induced in the early differentiation stage and reduced during the maturation stage of osteoblasts, when Cx43 expression increased in order to promote mineralization. Furthermore, only Panx3 functioned as an endoplasmic reticulum (ER) Ca2+ channel to promote differentiation, and it could rescue mineralization defects in Cx43(-/-) calvarial cells. Our findings reveal that Panx3 and Cx43 have distinct functions in skeletal formation.

DOI： 10.1242/jcs.176883

Dopaminergic differentiation of stem cells from human deciduous teeth and their therapeutic benefits for Parkinsonian rats Reviewed International journal

Hiromi Fujii, Kohki Matsubara, Kiyoshi Sakai, Mikako Ito, Kinji Ohno, Minoru Ueda, Akihito Yamamoto

BRAIN RESEARCH Vol. 1613 page： 59 - 72 2015.7

Language：English Publishing type：Research paper (scientific journal) Publisher：ELSEVIER SCIENCE BV

Parkinson's disease (PD) is a progressive neurodegenerative disorder caused by the loss of nigrostriatal dopaminergic (DAergic) neurons and the depletion of striatal dopamine. Here we show that DAergic-neuron-like cells could be efficiently induced from stem cells derived from human exfoliated deciduous teeth (SHEDS), and that these induced cells had therapeutic benefits in a 6-OHDA-induced Parkinsonian rat model. In our protocol, EGF and bFGF signaling activated the SHED's expression of proneural genes, Ngn2 and Mash1, and subsequent treatment with brain-derived neurotrophic factor (BDNF) promoted their maturation into DAergic neuron-like SHEDs (dSHEDs). A hypoxic DAergic differentiation protocol improved cell viability and enhanced the expression of multiple neurotrophic factors, including BDNF, GDNF, NT-3, and HGF. Engrafted dSHEDs survived in the striatum of Parldnsonian rats, improved the DA level more efficiently than engrafted undifferentiated SHEDs, and promoted the recovery from neurological deficits. Our findings further suggested that paracrine effects of dSHEDs contributed to neuroprotection against 6-OHDA-induced neurodegeneration and to nigrostriatal tract restoration. In addition, we found that the conditioned medium derived from dSHEDs protected primary neurons against 6-OHDA toxicity and accelerated neurite outgrowth in vitro. Thus, our data suggest that stem cells derived from dental pulp may have therapeutic benefits for PD. (C) 2015 Elsevier B.V. All rights reserved.

DOI： 10.1016/j.brainres.2015.04.001

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Secreted Ectodomain of Sialic Acid-Binding Ig-Like Lectin-9 and Monocyte Chemoattractant Protein-1 Promote Recovery after Rat Spinal Cord Injury by Altering Macrophage Polarity Reviewed International journal

Kohki Matsubara, Yoshihiro Matsushita, Kiyoshi Sakai, Fumiya Kano, Megumi Kondo, Mariko Noda, Noboru Hashimoto, Shiro Imagama, Naoki Ishiguro, Akio Suzumura, Minoru Ueda, Koichi Furukawa, Akihito Yamamoto

JOURNAL OF NEUROSCIENCE Vol. 35 ( 6 ) page： 2452 - 2464 2015.2

Language：English Publishing type：Research paper (scientific journal) Publisher：SOC NEUROSCIENCE

Engrafted mesenchymal stem cells from human deciduous dental pulp (SHEDs) support recovery from neural insults via paracrine mechanisms that are poorly understood. Here we show that the conditioned serum-free medium (CM) from SHEDs, administered intrathecally into rat injured spinal cord during the acute postinjury period, caused remarkable functional recovery. The ability of SHED-CM to induce recovery was associated with an immunoregulatory activity that induced anti-inflammatory M2-like macrophages. Secretome analysis of the SHED-CM revealed a previously unrecognized set of inducers for anti-inflammatory M2-like macrophages: monocyte chemoattractant protein-1 (MCP-1) and the secreted ectodomain of sialic acid-binding Ig-like lectin-9 (ED-Siglec-9). Depleting MCP-1 and ED-Siglec-9 from the SHED-CM prominently reduced its ability to induce M2-like macrophages and to promote functional recovery after spinal cord injury (SCI). The combination of MCP-1 and ED-Siglec-9 synergistically promoted the M2-like differentiation of bone marrow-derived macrophages in vitro, and this effect was abolished by a selective antagonist for CC chemokine receptor 2 (CCR2) or by the genetic knock-out of CCR2. Furthermore, MCP-1 and ED-Siglec-9 administration into the injured spinal cord induced M2-like macrophages and led to a marked recovery of hindlimb locomotor function after SCI. The inhibition of this M2 induction through the inactivation of CCR2 function abolished the therapeutic effects of both SHED-CM and MCP-1/ED-Siglec-9. Macrophages activated by MCP-1 and ED-Siglec-9 extended neurite and suppressed apoptosis of primary cerebellar granule neurons against the neurotoxic effects of chondroitin sulfate proteoglycans. Our data suggest that the unique combination of MCP-1 and ED-Siglec-9 repairs the SCI through anti-inflammatory M2-like macrophage induction.

DOI： 10.1523/JNEUROSCI.4088-14.2015

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Epiprofin orchestrates epidermal keratinocyte proliferation and differentiation Reviewed International journal

Takashi Nakamura, Yasuo Yoshitomi, Kiyoshi Sakai, Vyomesh Patel, Satoshi Fukumoto, Yoshihiko Yamada

JOURNAL OF CELL SCIENCE Vol. 127 ( 24 ) page： 5261 - 5272 2014.12

Language：English Publishing type：Research paper (scientific journal) Publisher：COMPANY OF BIOLOGISTS LTD

The basal layer of the epidermis contains stem cells and transit amplifying cells that rapidly proliferate and differentiate further into the upper layers of the epidermis. A number of molecules have been identified as regulators of this process, including p63 (also known as tumor protein 63) and Notch1. However, little is known about the mechanisms that regulate the transitions from stem cell to proliferating or differentiating transit amplifying cell. Here, we demonstrate that epiprofin (Epfn, also known as Sp6) plays crucial distinct roles in these transition stages as a cell cycle regulator and a transcription factor. Epfn knockout mice have a thickened epidermis, in which p63-expressing basal cells form multiple layers owing to the accumulation of premature transit amplifying cells with reduced proliferation and a reduction in the number of differentiating keratinocytes expressing Notch1. We found that low levels of Epfn expression increased the proliferation of human immortalized keratinocyte (HaCaT) cells by increasing EGF responsiveness and superphosphorylation of Rb. By contrast, high levels of Epfn expression promoted cell cycle exit and differentiation, by reducing E2F transactivation and inducing Notch1 expression. Our findings identify multiple novel functions of Epfn in epidermal development.

DOI： 10.1242/jcs.156778

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Ablation of Coactivator Med1 Switches the Cell Fate of Dental Epithelia to That Generating Hair Reviewed International coauthorship International journal

Keigo Yoshizaki, Lizhi Hu, Thai Nguyen, Kiyoshi Sakai, Bing He, Chak Fong, Yoshihiko Yamada, Daniel D. Bikle, Yuko Oda

PLOS ONE Vol. 9 ( 6 ) 2014.6

Language：English Publishing type：Research paper (scientific journal) Publisher：PUBLIC LIBRARY SCIENCE

Cell fates are determined by specific transcriptional programs. Here we provide evidence that the transcriptional coactivator, Mediator 1 (Med1), is essential for the cell fate determination of ectodermal epithelia. Conditional deletion of Med1 in vivo converted dental epithelia into epidermal epithelia, causing defects in enamel organ development while promoting hair formation in the incisors. We identified multiple processes by which hairs are generated in Med1 deficient incisors: 1) dental epithelial stem cells lacking Med 1 fail to commit to the dental lineage, 2) Sox2-expressing stem cells extend into the differentiation zone and remain multi-potent due to reduced Notch1 signaling, and 3) epidermal fate is induced by calcium as demonstrated in dental epithelial cell cultures. These results demonstrate that Med1 is a master regulator in adult stem cells to govern epithelial cell fate.

DOI： 10.1371/journal.pone.0099991

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Multifaceted neuro-regenerative activities of human dental pulp stem cells for functional recovery after spinal cord injury Reviewed International journal

Akihito Yamamoto, Kiyoshi Sakai, Kohki Matsubara, Fumiya Kano, Minoru Ueda

NEUROSCIENCE RESEARCH Vol. 78 page： 16 - 20 2014.1

Language：English Publishing type：Research paper (scientific journal) Publisher：ELSEVIER IRELAND LTD

Spinal cord injury (SCI) often leads to persistent functional deficits due to the loss of neurons and glia and to limited axonal regeneration after such injury. Recently, three independent groups have reported marked recovery of hindlimb locomotor function after the transplantation of human adult dental pulp stem cells (DPSCs) and stem cells from human exfoliated deciduous teeth (SHEDs) into rats or mice with acute, sub-acute or chronic SCI. This review summarizes the primary characteristics of human dental pulp stem cells and their therapeutic benefits for treating SCI. Experimental data from multiple preclinical studies suggest that pulp stem cells may promote functional recovery after SCI through multifaceted neuro-regenerative activities. (C) 2013 Elsevier Ireland Ltd and the Japan Neuroscience Society. All rights reserved.

DOI： 10.1016/j.neures.2013.10.010

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Analysis of the neuroregenerative activities of mesenchymal stem cells in functional recovery after rat spinal cord injury Reviewed International journal

A. Yamamoto, K. Matsubara, F. Kano, K. Sakai

Methods in Molecular Biology page： 321-328 2014

Analysis of the Neuroregenerative Activities of Mesenchymal Stem Cells in Functional Recovery after Rat Spinal Cord Injury International journal

Yamamoto Akihito, Matsubara Kohki, Kano Fumiya, Sakai Kiyoshi

ANIMAL MODELS FOR STEM CELL THERAPY Vol. 1213 page： 321 - 328 2014

Multifaceted Neuro-Regenerative Activities of Human Dental Pulp Stem Cells for Functional Recovery after Spinal Cord Injury International journal

Yamamoto Akihito, Matsubara Kohki, Sakai Kiyoshi, Kano Fumiya, Ueda Minoru

NEW TRENDS IN TISSUE ENGINEERING AND REGENERATIVE MEDICINE - OFFICIAL BOOK OF THE JAPANESE SOCIETY FOR REGENERATIVE MEDICINE page： 67 - 79 2014

Human Dental Pulp-Derived Stem Cells Protect Against Hypoxic-Ischemic Brain Injury in Neonatal Mice Reviewed International journal

Mari Yamagata, Akihito Yamamoto, Eisuke Kako, Naoko Kaneko, Kohki Matsubara, Kiyoshi Sakai, Kazunobu Sawamoto, Minoru Ueda

STROKE Vol. 44 ( 2 ) page： 551 - 554 2013.2

Language：English Publishing type：Research paper (scientific journal) Publisher：LIPPINCOTT WILLIAMS & WILKINS

Background and Purpose-Perinatal hypoxia-ischemia (HI) has high rates of neurological deficits and mortality. So far, no effective treatment for HI brain injury has been developed. In this study, we investigated the therapeutic effects of stem cells from human exfoliated deciduous teeth (SHED) for the treatment of neonatal HI brain injury.
Methods-Unilateral HI was induced in postnatal day 5 (P5) mice. Twenty-four hours later, SHED, human skin fibroblasts, or serum-free conditioned medium derived from these cells was injected into the injured brain. The effects of cell transplantation or conditioned medium injection on the animals' neurological and pathophysiological recovery were evaluated.
Results-Transplanted SHED, but not fibroblasts, significantly reduced the HI-induced brain-tissue loss and improved neurological function. SHED also improved the survival of the HI mice. The engrafted SHED rarely differentiated into neural lineages; however, their transplantation inhibited the expression of proinflammatory cytokines, increased the expression of anti-inflammatory ones, and significantly reduced apoptosis. Notably, the intracerebral administration of SHED-conditioned medium also significantly improved the neurological outcome, inhibited apoptosis, and reduced tissue loss.
Conclusions-SHED transplantation into the HI-injured brain resulted in remarkable neurological and pathophysiological recovery. Our findings indicate that paracrine factors derived from SHED support a neuroprotective microenvironment in the HI brain. SHED graft and SHED-conditioned medium may provide a novel neuroprotective therapy for HI. (Stroke. 2013;44:551-554.)

DOI： 10.1161/STROKEAHA.112.676759

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Human umbilical cord-derived mesenchymal stromal cells promote sensory recovery in a spinl cord injury rat model Reviewed International journal

S. Takikawa, A. Yamamoto, K. Sakai, R. Shohara, A. Iwase, F. Kikkawa, M. Ueda

Stem Cell Discovery page： 155-163 2013

Human dental pulp-derived stem cells promote locomotor recovery after complete transection of the rat spinal cord by multiple neuro-regenerative mechanisms Reviewed International journal

Kiyoshi Sakai, Akihito Yamamoto, Kohki Matsubara, Shoko Nakamura, Mami Naruse, Mari Yamagata, Kazuma Sakamoto, Ryoji Tauchi, Norimitsu Wakao, Shiro Imagama, Hideharu Hibi, Kenji Kadomatsu, Naoki Ishiguro, Minoru Ueda

JOURNAL OF CLINICAL INVESTIGATION Vol. 122 ( 1 ) page： 80 - 90 2012.1

Authorship：Lead author Language：English Publishing type：Research paper (scientific journal) Publisher：AMER SOC CLINICAL INVESTIGATION INC

Spinal cord injury (SCI) often leads to persistent functional deficits due to loss of neurons and glia and to limited axonal regeneration after injury. Here we report that transplantation of human dental pulp stem cells into the completely transected adult rat spinal cord resulted in marked recovery of hind limb locomotor functions. Transplantation of human bone marrow stromal cells or skin-derived fibroblasts led to substantially less recovery of locomotor function. The human dental pulp stem cells exhibited three major neuroregenerative activities. First, they inhibited the SCI-induced apoptosis of neurons, astrocytes, and oligodendrocytes, which improved the preservation of neuronal filaments and myelin sheaths. Second, they promoted the regeneration of transected axons by directly inhibiting multiple axon growth inhibitors, including chondroitin sulfate proteoglycan and myelin-associated glycoprotein, via paracrine mechanisms. Last, they replaced lost cells by differentiating into mature oligodendrocytes under the extreme conditions of SCI. Our data demonstrate that tooth-derived stem cells may provide therapeutic benefits for treating SCI through both cell-autonomous and paracrine neuroregenerative activities.

DOI： 10.1172/JCI59251

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Stability of a Locking Plate and Self-Drilling Screws as Orthodontic Skeletal Anchorage in the Maxilla: A Retrospective Study Reviewed International journal

Hidebaru Hibi, Kiyoshi Sakai, Tomoo Oda, Hisashi Hattori, Minoru Ueda, Masaru Sakai

JOURNAL OF ORAL AND MAXILLOFACIAL SURGERY Vol. 68 ( 8 ) page： 1783 - 1787 2010.8

Language：English Publishing type：Research paper (scientific journal) Publisher：W B SAUNDERS CO-ELSEVIER INC

Purpose: The aim of this retrospective study was to determine the success rate of an orthodontic skeletal anchorage system consisting of a locking plate and 2 self-drilling screws to intrude the upper molars and detect factors that contribute to its stability.
Patients and Methods: The subjects were 32 orthodontic and generally healthy patients who had skeletal anchorage plates placed supraperiosteally and unilaterally or bilaterally. The anchorage plate was considered successful if the plate remained stable throughout the period of intrusion of the upper molar without any movement, persistent pain, or infection and was then retrieved without difficulty. The success rates of the anchorage plate were statistically analyzed on the basis of clinically categorized variables.
Results: The 32 patients comprised 6 male and 26 female individuals with ages ranging from 11.4 to 35.1 years. The overall success rate of the total 61 plates was 93.4%. No significant differences were observed among the respective success rates analyzed in accordance with gender, age, side of placement, and length of the screws. The thickness of the bony walls that supported the screws was significantly greater in the success group (mean 1.6 +/- SD, 0.2 vs 1.0 +/- 0.1 mm, P < .001).
Conclusion: Bone thickness is a critical factor in supporting the self-drilling screws and locking plate. Skeletal anchorage combining the plate and 2 screws promises a higher success rate with a thicker bone than with the threshold value of thickness that exists within the 1.1 to 1 4 mm range in the maxillary walls. 2010 American Association of Oral and Maxillofacial Surgeons J Oral Maxillofac Surg 68:1783-1787, 2010

DOI： 10.1016/j.joms.2009.09.020

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多発性骨髄腫に対するビスフォスフォネート投与に関連したと考えられる上顎骨壊死の1例 International journal

酒井陽, 片桐渉, 服部宇, 金山健夫, 上田実, 山田陽一, 日比英晴, 伊藤憲治

日本口腔科学会雑誌 Vol. 57 ( 3 ) page： 334 - 334 2008.7

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Papers - SAKAI Kiyoshi