研究者詳細 - 酒井　陽

論文 - 酒井　陽

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Implant-supported fixed prostheses with cantilever: a systematic review and meta-analysis. 査読有り国際誌

Kondo Y, Sakai K, Minakuchi H, Horimai T, Kuboki T, JSOI Clinical Guideline Working Group collaborators

International journal of implant dentistry 10 巻 ( 1 ) 頁： 57 2024年11月

Conditioned medium of human mesenchymal stem cells affects stem cell senescence in osteoporosis. 査読有り国際共著国際誌

Liu K, Sakai K, Watanabe J, Dong J, Maruyama H, Li X, Hibi H

Biochemical and biophysical research communications 711 巻頁： 149858 2024年4月

Cytoprotective role of human dental pulp stem cell-conditioned medium in chemotherapy-induced alopecia. 査読有り国際誌

Chen H, Yamaguchi S, Wang Y, Kaminogo K, Sakai K, Hibi H

Stem cell research & therapy 15 巻 ( 1 ) 頁： 84 2024年3月

Cellular senescence is associated with osteonecrosis of the femoral head while mesenchymal stem cell conditioned medium inhibits bone collapse. 査読有り国際誌

Okamoto M, Nakashima H, Sakai K, Takegami Y, Osawa Y, Watanabe J, Ito S, Hibi H, Imagama S

Scientific reports 14 巻 ( 1 ) 頁： 3329 2024年2月

担当区分：責任著者記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Scientific Reports

Osteonecrosis of the femoral head (ONFH) is a type of ischemic osteonecrosis that causes pain, loss of function, and femoral head collapse. Here, we analyzed samples of femoral heads excised from patients with ONFH to clarify the relationship between ischemic osteonecrosis and cellular senescence. X-gal staining was strong and p16INK4a-positive cells were abundant in the transitional region of ONFH. The β-galactosidase-positive cells in the transitional region were also positive for nestin, periostin, or DMP-1. In contrast, no β-galactosidase-positive cells were detected in the healthy region. The senescence-associated p16INK4a, p21, and p53 were upregulated in ONFH tissue. We also examined and analyzed a mouse ischemic femoral osteonecrosis model in vivo to verify the association between ONFH and cellular senescence. Human mesenchymal stem cell-conditioned medium (MSC-CM) was administered to determine its therapeutic efficacy against cellular senescence and bone collapse. MSC-CM reduced the number of senescent cells and downregulated the aforementioned senescence-related genes. It also decreased the number of empty lacunae 4 weeks after ischemia induction and promoted bone formation. At 6 weeks post-surgery, MSC-CM increased the trabecular bone volume, thereby suppressing bone collapse. We conclude that cellular senescence is associated with ONFH and that MSC-CM suppresses bone collapse in this disorder.

DOI： 10.1038/s41598-024-53400-w

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PubMed

Langerhans cell histiocytosis of single-system multifocal bone, including the mandible, in a 22-month-old child: A case report 査読有り国際誌

Kiyoshi Sakai, Noriyuki Yamamoto, Satoshi Yamaguchi, Kazuto Okabe, Yoshiro Koma, Hideharu Hibi

Oral Science International 2022年6月

Dental pulp stem cell-derived small extracellular vesicle in irradiation-induced senescence 査読有り国際共著国際誌

Dong Jiao, Sakai Kiyoshi, Koma Yoshiro, Watanabe Junna, Liu Kehong, Maruyama Hiroshi, Sakaguchi Kohei, Hibi Hideharu

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 575 巻頁： 28 - 35 2021年10月

Conditioned medium from stem cells derived from human exfoliated deciduous teeth ameliorates NASH via the Gut-Liver axis. 査読有り国際誌

Muto H, Ito T, Tanaka T, Yokoyama S, Yamamoto K, Imai N, Ishizu Y, Maeda K, Honda T, Ishikawa T, Kato A, Ohshiro T, Kano F, Yamamoto A, Sakai K, Hibi H, Ishigami M, Fujishiro M

Scientific reports 11 巻 ( 1 ) 頁： 18778 2021年9月

Coexistence of Sjogren's syndrome and bilateral ranulas: A case report and review of the literature 査読有り国際誌

Koma Yoshiro, Fujimoto Takehiro, Sakai Kiyoshi, Sugimura Yukiko, Yamaguchi Satoshi, Hibi Hideharu

ORAL SCIENCE INTERNATIONAL 2021年9月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Oral Science International

Sjögren's syndrome and ranulas are relatively frequently occurring diseases. However, when they occur simultaneously, its clinical features and pathogenesis remain unclear. Here, we report a patient with bilateral ranulas. Magnetic resonance imaging at diagnosis revealed a “salt and pepper” appearance in bilateral parotid glands, leading a diagnosis of asymptomatic Sjögren's syndrome. She was successfully treated with excision of the left sublingual gland and sclerotherapy. A literature review suggests that this coexistence may not be very rare. In addition, it may be characterized by a high prevalence of bilateral ranulas.

DOI： 10.1002/osi2.1121

Web of Science

Scopus

Hyperparathyroidism-jaw tumor syndrome with bilateral ossifying fibromas in the mandible: A case report 査読有り国際誌

Journal of Oral and Maxillofacial Surgery, Medicine, and Pathology 2021年7月

Extracellular Vesicles of Stem Cells to Prevent BRONJ 査読有り国際誌

Watanabe J., Sakai K., Urata Y., Toyama N., Nakamichi E., Hibi H.

JOURNAL OF DENTAL RESEARCH 99 巻 ( 5 ) 頁： 552 - 560 2020年5月

先天性無フィブリノゲン血症患者の抜歯術における周術期管理を行った1例査読有り

岡部一登, 土屋周平, 酒井陽, 松下義泰, 杉本圭佑, 日比英晴

日本口腔外科学会雑誌 65 巻 ( 8 ) 頁： 507 - 512 2019年8月

記述言語：日本語掲載種別：研究論文（学術雑誌）出版者・発行元：(公社)日本口腔外科学会

13歳男子。右下6番根尖性歯周炎に起因した下顎骨骨膜下膿瘍および頬部蜂窩織炎と診断した。先天性無フィブリノゲン(Fbg)血症のため消炎処置前にFbg製剤2gを投与し、歯肉の腫脹部より膿瘍切開術を施行した。同日よりアモキシシリン投与を開始し、翌日より切開部からの洗浄を継続し、7日後には顎下部や口腔内の腫脹は消退した。翌月に右下6番を抜歯し、抜歯後は事前に製作した創部保護床を終日装着させた。Fbg製剤は血中Fbg濃度100mg/dL以上を目標に抜歯当日3g、抜歯後1日目に2g、抜歯後2日目に2g投与した。抜歯後2日目には抜歯窩の縫合糸は脱落していたが幼若な血餅で満たされ、引き続き創部保護床は終日装着したままとして退院した。抜歯後8日目に創部保護床を除去し、処置後の異常出血はなく、治癒の遅延も認めなかった。

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Identification of the Novel Tooth-Specific Transcription Factor AmeloD 査読有り国際共著

He B., Chiba Y., Li H., de Vega S., Tanaka K., Yoshizaki K., Ishijima M., Yuasa K., Ishikawa M., Rhodes C., Sakai K., Zhang P., Fukumoto S., Zhou X., Yamada Y.

JOURNAL OF DENTAL RESEARCH 98 巻 ( 2 ) 頁： 234 - 241 2019年2月

Dental pulp-derived stem cell conditioned medium to regenerate peripheral nerves in a novel animal model of dysphagia 査読有り国際誌

Tsuruta Takeshi, Sakai Kiyoshi, Watanabe Junna, Katagiri Wataru, Hibi Hideharu

PLOS ONE 13 巻 ( 12 ) 頁： e0208938 2018年12月

他家骨髄由来間葉系細胞の移植による骨再生の検討

渡邊純奈, 片桐渉, 大杉将嗣, 酒井陽, 岡部一登, 椙村有紀子, 坂口晃平, 鶴田剛士, 日比英晴

日本口腔インプラント学会誌 31 巻 ( 2 ) 頁： E75 - E75 2018年6月

Mediator 1 Contributes to Enamel Mineralization as a Coactivator for Notch1 Signaling and Stimulates Transcription of the Alkaline Phosphatase Gene 査読有り国際共著

292 巻 ( 33 ) 頁： 13531-13540 2017年8月

ラット末梢性嚥下障害モデルを用いた乳歯歯髄幹細胞由来成長因子の治療効果の検討国際誌

鶴田剛士, 片桐渉, 大杉将嗣, 酒井陽, 若山有紀子, 坂口晃平, 渡邊純奈, 日比英晴

日本口腔科学会雑誌 66 巻 ( 2 ) 頁： 207 - 208 2017年7月

Pannexin 3 and connexin 43 modulate skeletal development through their distinct functions and expression patterns 査読有り国際共著国際誌

Ishikawa Masaki, Williams Geneva L., Ikeuchi Tomoko, Sakai Kiyoshi, Fukumoto Satoshi, Yamada Yoshihiko

JOURNAL OF CELL SCIENCE 129 巻 ( 5 ) 頁： 1018 - 1030 2016年3月

Dopaminergic differentiation of stem cells from human deciduous teeth and their therapeutic benefits for Parkinsonian rats 査読有り国際誌

Hiromi Fujii, Kohki Matsubara, Kiyoshi Sakai, Mikako Ito, Kinji Ohno, Minoru Ueda, Akihito Yamamoto

BRAIN RESEARCH 1613 巻頁： 59 - 72 2015年7月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：ELSEVIER SCIENCE BV

Parkinson's disease (PD) is a progressive neurodegenerative disorder caused by the loss of nigrostriatal dopaminergic (DAergic) neurons and the depletion of striatal dopamine. Here we show that DAergic-neuron-like cells could be efficiently induced from stem cells derived from human exfoliated deciduous teeth (SHEDS), and that these induced cells had therapeutic benefits in a 6-OHDA-induced Parkinsonian rat model. In our protocol, EGF and bFGF signaling activated the SHED's expression of proneural genes, Ngn2 and Mash1, and subsequent treatment with brain-derived neurotrophic factor (BDNF) promoted their maturation into DAergic neuron-like SHEDs (dSHEDs). A hypoxic DAergic differentiation protocol improved cell viability and enhanced the expression of multiple neurotrophic factors, including BDNF, GDNF, NT-3, and HGF. Engrafted dSHEDs survived in the striatum of Parldnsonian rats, improved the DA level more efficiently than engrafted undifferentiated SHEDs, and promoted the recovery from neurological deficits. Our findings further suggested that paracrine effects of dSHEDs contributed to neuroprotection against 6-OHDA-induced neurodegeneration and to nigrostriatal tract restoration. In addition, we found that the conditioned medium derived from dSHEDs protected primary neurons against 6-OHDA toxicity and accelerated neurite outgrowth in vitro. Thus, our data suggest that stem cells derived from dental pulp may have therapeutic benefits for PD. (C) 2015 Elsevier B.V. All rights reserved.

DOI： 10.1016/j.brainres.2015.04.001

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Secreted Ectodomain of Sialic Acid-Binding Ig-Like Lectin-9 and Monocyte Chemoattractant Protein-1 Promote Recovery after Rat Spinal Cord Injury by Altering Macrophage Polarity 査読有り国際誌

Kohki Matsubara, Yoshihiro Matsushita, Kiyoshi Sakai, Fumiya Kano, Megumi Kondo, Mariko Noda, Noboru Hashimoto, Shiro Imagama, Naoki Ishiguro, Akio Suzumura, Minoru Ueda, Koichi Furukawa, Akihito Yamamoto

JOURNAL OF NEUROSCIENCE 35 巻 ( 6 ) 頁： 2452 - 2464 2015年2月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：SOC NEUROSCIENCE

Engrafted mesenchymal stem cells from human deciduous dental pulp (SHEDs) support recovery from neural insults via paracrine mechanisms that are poorly understood. Here we show that the conditioned serum-free medium (CM) from SHEDs, administered intrathecally into rat injured spinal cord during the acute postinjury period, caused remarkable functional recovery. The ability of SHED-CM to induce recovery was associated with an immunoregulatory activity that induced anti-inflammatory M2-like macrophages. Secretome analysis of the SHED-CM revealed a previously unrecognized set of inducers for anti-inflammatory M2-like macrophages: monocyte chemoattractant protein-1 (MCP-1) and the secreted ectodomain of sialic acid-binding Ig-like lectin-9 (ED-Siglec-9). Depleting MCP-1 and ED-Siglec-9 from the SHED-CM prominently reduced its ability to induce M2-like macrophages and to promote functional recovery after spinal cord injury (SCI). The combination of MCP-1 and ED-Siglec-9 synergistically promoted the M2-like differentiation of bone marrow-derived macrophages in vitro, and this effect was abolished by a selective antagonist for CC chemokine receptor 2 (CCR2) or by the genetic knock-out of CCR2. Furthermore, MCP-1 and ED-Siglec-9 administration into the injured spinal cord induced M2-like macrophages and led to a marked recovery of hindlimb locomotor function after SCI. The inhibition of this M2 induction through the inactivation of CCR2 function abolished the therapeutic effects of both SHED-CM and MCP-1/ED-Siglec-9. Macrophages activated by MCP-1 and ED-Siglec-9 extended neurite and suppressed apoptosis of primary cerebellar granule neurons against the neurotoxic effects of chondroitin sulfate proteoglycans. Our data suggest that the unique combination of MCP-1 and ED-Siglec-9 repairs the SCI through anti-inflammatory M2-like macrophage induction.

DOI： 10.1523/JNEUROSCI.4088-14.2015

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Epiprofin orchestrates epidermal keratinocyte proliferation and differentiation 査読有り国際誌

Takashi Nakamura, Yasuo Yoshitomi, Kiyoshi Sakai, Vyomesh Patel, Satoshi Fukumoto, Yoshihiko Yamada

JOURNAL OF CELL SCIENCE 127 巻 ( 24 ) 頁： 5261 - 5272 2014年12月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：COMPANY OF BIOLOGISTS LTD

The basal layer of the epidermis contains stem cells and transit amplifying cells that rapidly proliferate and differentiate further into the upper layers of the epidermis. A number of molecules have been identified as regulators of this process, including p63 (also known as tumor protein 63) and Notch1. However, little is known about the mechanisms that regulate the transitions from stem cell to proliferating or differentiating transit amplifying cell. Here, we demonstrate that epiprofin (Epfn, also known as Sp6) plays crucial distinct roles in these transition stages as a cell cycle regulator and a transcription factor. Epfn knockout mice have a thickened epidermis, in which p63-expressing basal cells form multiple layers owing to the accumulation of premature transit amplifying cells with reduced proliferation and a reduction in the number of differentiating keratinocytes expressing Notch1. We found that low levels of Epfn expression increased the proliferation of human immortalized keratinocyte (HaCaT) cells by increasing EGF responsiveness and superphosphorylation of Rb. By contrast, high levels of Epfn expression promoted cell cycle exit and differentiation, by reducing E2F transactivation and inducing Notch1 expression. Our findings identify multiple novel functions of Epfn in epidermal development.

DOI： 10.1242/jcs.156778

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